keyword
https://read.qxmd.com/read/38536087/-peptostreptococcus-equinus-sp-nov-isolated-from-horse-faeces
#21
JOURNAL ARTICLE
Hye Su Jung, Jeong Ui Yun, Mi-Ja Jung, Hye Seon Song, Yeon Bee Kim, Yujin Kim, Min Ji Lee, Jisu Lee, Che Ok Jeon, Seong Woon Roh, Tae Woong Whon, Joon-Gyu Kim, Se Hee Lee
A Gram-stain-positive, non-spore-forming, and obligate anaerobic bacteria designated strain CBA3647T was isolated from a horse faecal sample in Jeju, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain CBA3647T formed a distinct phyletic lineage from closely related species within the genus Peptostreptococcus . Based on comparative analysis of 16S rRNA gene sequences, Peptostreptococcus anaerobius ATCC 27337T is most closely related to strain CBA3647T with a 16S rRNA gene similarity of 98...
March 2024: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/38536078/genetic-associations-between-circulating-immune-cells-and-periodontitis-highlight-the-prospect-of-systemic-immunoregulation-in-periodontal-care
#22
JOURNAL ARTICLE
Xinjian Ye, Yijing Bai, Mengjun Li, Yuhang Ye, Yitong Chen, Bin Liu, Yuwei Dai, Shan Wang, Weiyi Pan, Zhiyong Wang, Yingying Mao, Qianming Chen
Periodontitis drives irreversible destruction of periodontal tissue and is prone to exacerbating inflammatory disorders. Systemic immunomodulatory management continues to be an attractive approach in periodontal care, particularly within the context of 'predictive, preventive, and personalized' periodontics. The present study incorporated genetic proxies identified through genome-wide association studies for circulating immune cells and periodontitis into a comprehensive Mendelian randomization (MR) framework...
March 27, 2024: ELife
https://read.qxmd.com/read/38536071/-xanthomonas-rydalmerensis-sp-nov-a-non-pathogenic-member-of-group-1-xanthomonas
#23
JOURNAL ARTICLE
Daniel J E McKnight, Johanna Wong-Bajracharya, Efenaide B Okoh, Fridtjof Snijders, Fiona Lidbetter, John Webster, Mathew Haughton, Aaron E Darling, Steven P Djordjevic, Daniel R Bogema, Toni A Chapman
Five bacterial isolates were isolated from Fragaria × ananassa in 1976 in Rydalmere, Australia, during routine biosecurity surveillance. Initially, the results of biochemical characterisation indicated that these isolates represented members of the genus Xanthomonas . To determine their species, further analysis was conducted using both phenotypic and genotypic approaches. Phenotypic analysis involved using MALDI-TOF MS and BIOLOG GEN III microplates, which confirmed that the isolates represented members of the genus Xanthomonas but did not allow them to be classified with respect to species...
March 2024: International Journal of Systematic and Evolutionary Microbiology
https://read.qxmd.com/read/38535814/development-of-a-rapid-and-sensitive-canary-biosensor-assay-for-the-detection-of-shiga-toxin-2-from-escherichia-coli
#24
JOURNAL ARTICLE
Christina C Tam, Yangyang Wang, Wen-Xian Du, Andrew R Flannery, Xiaohua He
Shiga-toxin-producing Escherichia coli (STEC) causes a wide spectrum of diseases including hemorrhagic colitis and hemolytic uremic syndrome (HUS). The current Food Safety Inspection Service (FSIS) testing methods for STEC use the Food and Drug Administration (FDA) Bacteriological Analytical Manual (BAM) protocol, which includes enrichment, cell plating, and genomic sequencing and takes time to complete, thus delaying diagnosis and treatment. We wanted to develop a rapid, sensitive, and potentially portable assay that can identify STEC by detecting Shiga toxin (Stx) using the CANARY (Cellular Analysis and Notification of Antigen Risks and Yields) B-cell based biosensor technology...
March 14, 2024: Toxins
https://read.qxmd.com/read/38535607/metagenomic-detection-and-genetic-characterization-of-human-sapoviruses-among-children-with-acute-flaccid-paralysis-in-nigeria
#25
JOURNAL ARTICLE
Uwem Etop George, Temitope O C Faleye, Lander De Coninck, Sheriff Tunde Agbaje, Ijeoma Maryjoy Ifeorah, Bernard Anyebe Onoja, Elijah Igbekele Oni, Adebowale Oluseyi Olayinka, Toluwani Goodnews Ajileye, Arthur Obinna Oragwa, Toluwanimi Emmanuel Akinleye, Bolutife Olubukola Popoola, Oluwadamilola Gideon Osasona, Olaitan Titilola Olayinka, Oluwadamilola Adefunke George, Ahmed Iluoreh Muhammad, Isaac Komolafe, Adekunle Johnson Adeniji, Jelle Matthijnssens, Moses Olubusuyi Adewumi
Using a metagenomic sequencing approach on stool samples from children with Acute Flaccid Paralysis (AFP), we describe the genetic diversity of Sapoviruses (SaVs) in children in Nigeria. We identified six complete genome sequences and two partial genome sequences. Several SaV genogroups and genotypes were detected, including GII (GII.4 and GII.8), GIV (GIV.1), and GI (GI.2 and GI.7). To our knowledge, this is the first description of SaV infections and complete genomes from Nigeria. Pairwise identity and phylogenetic analysis showed that the Nigerian SaVs were related to previously documented gastroenteritis outbreaks with associated strains from China and Japan...
March 19, 2024: Pathogens
https://read.qxmd.com/read/38535604/genome-sequencing-of-historical-encephalomyocarditis-viruses-from-south-africa-links-the-historical-1993-4-savanna-elephant-loxodonta-africana-outbreak-to-cryptic-mastomys-rodents
#26
JOURNAL ARTICLE
Vanessa van Meer, Janusz T Pawęska, Robert Swanepoel, Antoinette Grobbelaar, Armanda D Bastos
From 1993 to 1994, 64 free-ranging elephants ( Loxodonta africana ) succumbed to encephalomyocarditis in the Kruger National Park, South Africa, of which 83% were adult bulls. Mastomys rodents were implicated as the reservoir host of the Encephalomyocarditis virus (EMCV) based on serology and RT-PCR. However, in the absence of sequence-confirmation of both the virus and the rodent host, definitive links between the elephant outbreak strains and rodent reservoir could not be established. In this study, we generate the first reference genome sequences for three historical EMCVs isolated from two Mastomys rodents and one Mastomys -associated mite, Laelaps muricola , in Gauteng Province, South Africa, in 1961...
March 19, 2024: Pathogens
https://read.qxmd.com/read/38535593/characterization-of-a-novel-species-of-legionella-isolated-from-a-healthcare-facility-legionella-resiliens-sp-nov
#27
JOURNAL ARTICLE
Sandra Cristino, Maria Rosaria Pascale, Federica Marino, Carlo Derelitto, Silvano Salaris, Massimiliano Orsini, Stefano Squarzoni, Antonella Grottola, Luna Girolamini
Two Legionella -like isolates, 8cVS16T and 9fVS26, were isolated from a water distribution system (WDS) in a healthcare facility. Cells were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, motile, and exhibited a blue-white fluorescence under Wood's lamp at 365 nm. The strains grew in a range of 32-37 °C on BCYE with L-cysteine (C ys +), GVPC, and MWY agar medium, with a positive reaction for oxidase, catalase, and gelatinase. The dominant fatty acids were summed features 3 (C16:1 ω7c/C16:1 ω6c) (27...
March 14, 2024: Pathogens
https://read.qxmd.com/read/38535535/feline-chronic-gingivostomatitis-diagnosis-and-treatment-through-transcriptomic-insights
#28
Maria Soltero-Rivera, Claire Shaw, Boaz Arzi, Milinda Lommer, Bart C Weimer
Feline chronic gingivostomatitis (FCGS) is a debilitating inflammatory oral mucosal disease with a multifactorial etiology. The clinical diagnosis of FCGS is made based on inspection of severe inflammatory lesions and histological confirmation rather than a molecular diagnostic outcome. This gap limits the ability to provide an early diagnosis. In this report, we seek to provide additional diagnostic tools using genomics to aid in providing clinically relevant information. The use of in-depth diagnostic tools, like transcriptomics of diseased tissues, to diagnose FCGS and stratify patients into predictive treatment response groups would dramatically improve both clinical decisions and patient outcomes...
February 21, 2024: Pathogens
https://read.qxmd.com/read/38535496/mitigation-of-salt-stress-in-rice-by-the-halotolerant-plant-growth-promoting-bacterium-enterobacter-asburiae-d2
#29
JOURNAL ARTICLE
Zican Ning, Kexin Lin, Mengya Gao, Xiao Han, Qingjie Guan, Xiang Ji, Shuyu Yu, Lei Lu
Salinity is a major abiotic stress that seriously affects crop growth worldwide. In this work, we aimed to isolate potential halotolerant plant growth-promoting rhizobacteria (PGPR) to mitigate the adverse impacts of salt stress in rice. An isolate, D2, with multiple plant growth-promoting (PGP) characteristics was identified as Enterobacter asburiae D2. Strain D2 could produce indole-3-acetic acid and siderophore. It also exhibited phosphate solubilization and 1-aminocyclopropane-1-carboxylic deaminase activity...
March 1, 2024: Journal of Xenobiotics
https://read.qxmd.com/read/38535493/the-complete-genome-sequence-of-bacillus-toyonensis-cbmb3-with-polyvinyl-chloride-degrading-properties
#30
JOURNAL ARTICLE
Dandan Wang, Hong Yu, Xinbei Liu, Li Sun, Xijian Liu, Ruilong Hu, Chao Wang, Yuping Zhuge, Zhihong Xie
The accumulation of high amounts of plastic waste in the environment has raised ecological and health concerns, particularly in croplands, and biological degradation presents a promising approach for the sustainable treatment of this issue. In this study, a polyvinyl chloride (PVC)-degrading bacterium was isolated from farmland soil samples attached to waste plastic, utilizing PVC as the sole carbon source. The circular chromosome of the strain Cbmb3, with a length of 5,768,926 bp, was subsequently sequenced...
February 26, 2024: Journal of Xenobiotics
https://read.qxmd.com/read/38535370/reassessment-of-the-phylogenetics-of-two-pygmy-grasshopper-generic-groups-tetrix-and-systolederus-through-mitochondrial-phylogenomics-using-four-new-mitochondrial-genome-assemblies
#31
JOURNAL ARTICLE
De-Long Guan, Chao-Mei Huang, Wei-An Deng
Mitochondrial genomes offer pragmatic genetic markers to reconstruct evolutionary relationships and inform taxonomic classifications. Here, we present complete mitochondrial sequences for four Chinese pygmy grasshoppers (Tetrigidae), aiming to reevaluate phylogenetic patterns and morphological taxonomy. Our 17,643 bp, 16,274 bp, 15,086 bp, and 15,398 bp mitogenomes of Exothotettix guangxiensis , Formosatettix longwangshanensis , Euparatettix sinufemoralis and Systolederus zhengi , respectively, exhibit archetypal Tetrigidae architecture...
March 4, 2024: Insects
https://read.qxmd.com/read/38535342/genome-wide-exploration-of-long-non-coding-rnas-of-helicoverpa-armigera-in-response-to-pyrethroid-insecticide-resistance
#32
JOURNAL ARTICLE
Md-Mafizur Rahman, Celso Omoto, Juil Kim
Genome-wide long non-coding RNAs (lncRNAs) in low, moderate, and high pyrethroid insecticide-resistant and -susceptible strains of Helicoverpa armigera were identified in this study. Using 45 illumina-based RNA-sequencing datasets, 8394 lncRNAs were identified. In addition, a sublethal dose of deltamethrin was administered to a Korean-resistant strain (Kor-T). The average length of lncRNAs was approximately 531 bp, and the expression ratio of lncRNAs was 28% of the total RNA. The identified lncRNAs were divided into six categories-intronic, intergenic, sense, antisense, cis-RNA, and trans-RNA-based on their location and mechanism of action...
February 21, 2024: Insects
https://read.qxmd.com/read/38535323/in-silico-analysis-of-novel-bacterial-metabolites-with-anticancer-activities
#33
JOURNAL ARTICLE
Pfariso Maumela, Mahloro Hope Serepa-Dlamini
Resistance to anticancer therapeutics is a major global concern. Thus, new anticancer agents should be aimed against novel protein targets to effectively mitigate the increased resistance. This study evaluated the potential of secondary metabolites from a bacterial endophyte, as new anticancer agents, against a novel protein target, fibroblast growth factor. In silico genomic characterization of the Bacillus sp. strain MHSD_37 was used to identify potential genes involved in encoding secondary metabolites with biological activity...
March 13, 2024: Metabolites
https://read.qxmd.com/read/38535317/integrative-multiomics-approach-to-skin-the-sinergy-between-individualised-medicine-and-futuristic-precision-skin-care
#34
REVIEW
Angelica Dessì, Roberta Pintus, Vassilios Fanos, Alice Bosco
The skin is a complex ecosystem colonized by millions of microorganisms, the skin microbiota, which are crucial in regulating not only the physiological functions of the skin but also the metabolic changes underlying the onset of skin diseases. The high microbial colonization together with a low diversity at the phylum level and a high diversity at the species level of the skin is very similar to that of the gastrointestinal tract. Moreover, there is an important communication pathway along the gut-brain-skin axis, especially associated with the modulation of neurotransmitters by the microbiota...
March 7, 2024: Metabolites
https://read.qxmd.com/read/38535312/integrating-genome-sequencing-and-untargeted-metabolomics-in-monozygotic-twins-with-a-rare-complex-neurological-disorder
#35
Rulan Shaath, Aljazi Al-Maraghi, Haytham Ali, Jehan AlRayahi, Adam D Kennedy, Karen L DeBalsi, Sura Hussein, Najwa Elbashir, Sujitha S Padmajeya, Sasirekha Palaniswamy, Sarah H Elsea, Ammira A Akil, Noha A Yousri, Khalid A Fakhro
Multi-omics approaches, which integrate genomics, transcriptomics, proteomics, and metabolomics, have emerged as powerful tools in the diagnosis of rare diseases. We used untargeted metabolomics and whole-genome sequencing (WGS) to gain a more comprehensive understanding of a rare disease with a complex presentation affecting female twins from a consanguineous family. The sisters presented with polymicrogyria, a Dandy-Walker malformation, respiratory distress, and multiorgan dysfunctions. Through WGS, we identified two rare homozygous variants in both subjects, a pathogenic variant in ADGRG1 (p...
March 4, 2024: Metabolites
https://read.qxmd.com/read/38535251/gold-nanoparticle-based-colorimetric-and-fluorescent-dual-mode-lateral-flow-immunoassay-for-sars-cov-2-detection
#36
JOURNAL ARTICLE
Ying Gan, Hefan Zhang, Jing Liu, Fuqin He, Fengheng Li, Ao Li, Man Xing, Dongming Zhou, Shan-Yu Fung, Hong Yang
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection caused the COVID-19 pandemic, impacting the global economy and medical system due to its fast spread and extremely high infectivity. Efficient control of the spread of the disease relies on a fast, accurate, and convenient detection system for the early screening of the infected population. Although reverse transcription-quantitative polymerase chain reaction (RT-qPCR) is the gold-standard method for SARS-CoV-2 RNA analysis, it has complex experimental procedures and relies on expensive instruments and professional operators...
February 27, 2024: Journal of Functional Biomaterials
https://read.qxmd.com/read/38535219/chromosome-level-assembly-and-comparative-genomic-analysis-of-suillus-bovinus-provides-insights-into-the-mechanism-of-mycorrhizal-symbiosis
#37
JOURNAL ARTICLE
Jinhua Zhang, Mengya An, Yanliu Chen, Shengkun Wang, Junfeng Liang
Suillus bovinus is a wild edible ectomycorrhizal fungus with important economic and ecological value, which often forms an ectomycorrhiza with pine trees. We know little about the mechanisms associated with the metabolism and symbiosis of S. bovinus and its effects on the nutritional value. In this study, the whole-genome sequencing of S. bovinus was performed using Illumina, HiFi, and Hi-C technologies, and the sequencing data were subjected to genome assembly, gene prediction, and functional annotation to obtain a high-quality chromosome-level genome of S...
March 13, 2024: Journal of Fungi (Basel, Switzerland)
https://read.qxmd.com/read/38535218/importance-of-the-aspergillus-fumigatus-mismatch-repair-protein-msh6-in-antifungal-resistance-development
#38
JOURNAL ARTICLE
Jose Lucio, Irene Gonzalez-Jimenez, Alejandra Roldan, Jorge Amich, Laura Alcazar-Fuoli, Emilia Mellado
One of the systems responsible for the recognition and repair of mistakes occurring during cell replication is the DNA mismatch repair (MMR) system. Two major protein complexes constitute the MMR pathway: MutS and MutL. Here, we investigated the possible relation of four A. fumigatus MMR genes ( msh 2, msh 6, pms 1, and mlh 1) with the development of azole resistance related to the phenomenon of multi-drug resistance. We examined the MMR gene variations in 163 Aspergillus fumigatus genomes. Our analysis showed that genes msh 2, pms 1, and mlh 1 have low genetic variability and do not seem to correlate with drug resistance...
March 12, 2024: Journal of Fungi (Basel, Switzerland)
https://read.qxmd.com/read/38535189/genome-analysis-of-a-newly-discovered-yeast-species-hanseniaspora-menglaensis
#39
JOURNAL ARTICLE
Adam P Ryan, Marizeth Groenewald, Maudy Th Smith, Cian Holohan, Teun Boekhout, Kenneth H Wolfe, Geraldine Butler
Annual surveys of Irish soil samples identified three isolates, CBS 16921 (UCD88), CBS 18246 (UCD443), and CBS 18247 (UCD483), of an apiculate yeast species within the Hanseniaspora genus. The internal transcribed spacer (ITS) and D1/D2 region of the large subunit (LSU) rRNA sequences showed that these are isolates of the recently described species Hanseniaspora menglaensis , first isolated from Southwest China. No genome sequence for H. menglaensis is currently available. The genome sequences of the three Irish isolates were determined using short-read (Illumina) sequencing, and the sequence of one isolate (CBS 16921) was assembled to chromosome level using long-read sequencing (Oxford Nanopore Technologies)...
February 28, 2024: Journal of Fungi (Basel, Switzerland)
https://read.qxmd.com/read/38535187/comparative-transcriptome-profiles-of-the-response-of-mycelia-of-the-genus-morchella-to-temperature-stress-an-examination-of-potential-resistance-mechanisms
#40
JOURNAL ARTICLE
Yihong Yue, Haibo Hao, Qian Wang, Tingting Xiao, Yuchen Zhang, Hui Chen, Jinjing Zhang
Temperature and moisture belong to the most important environmental factors affecting the growth and development of fungi. However, the effect of temperature on the mycelia of the edible Morchella mushrooms has not been determined. Here, a comprehensive analysis was performed to determine the influence of culture temperature on 13 strains of mycelia of three Morchella species ( Morchella sextelata , Morchella septimelata , and Morchella importuna ) at 5 °C, 10 °C, 15 °C, 20 °C, 25 °C, and 30 °C...
February 27, 2024: Journal of Fungi (Basel, Switzerland)
keyword
keyword
16873
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.