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https://www.readbyqxmd.com/read/30537176/functional-analysis-of-dna-methylation-of-the-pacsin1-promoter-in-pig-peripheral-blood-mononuclear-cells
#1
Wen Feng, Lei Zhou, Haifei Wang, Zhengzheng Hu, Xiaomei Wang, Jianlian Fu, Aiguo Wang, Jian-Feng Liu
DNA methylation plays essential roles in regulating the activity of genes and may contribute to understanding the potential epigenetic biomarkers response to viruses. To explore the function of DNA methylation of protein kinase C and casein kinase substrate in neurons 1 (PACSNI1) promoter, herein we performed the bisulfite sequencing polymerase chain reaction and Western blot analysis to verify hypermethylation and downregulation of PACSIN1 expression in peripheral blood mononuclear cells of pig as the vitro model...
December 9, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/30532488/nucleolar-organizer-region-banding-in-crossbred-and-non-descript-pigs-of-india
#2
V Harshini, K Sakunthala Devi, B Punya Kumari, J Suresh
Aim: The objective of this experiment was to study the nucleolar organizer region (NOR)-banding pattern in Large White Yorkshire (LWY) crossbred and non-descript pigs and finding differences in the number of NORs between animals and between genetic groups. Materials and Methods: The experiment was carried out on 15 females, and 15 males of LWY crossbred and non-descript pigs to study NOR-banding pattern by employing ammoniacal silver staining technique. Results: A total of 63 and 65 number of good metaphases were prepared in LWY crossbred, and non-descript pigs and a total of 168 and 143 number of NORs were detected on the 8th and 10th chromosomes in both genetic groups, respectively...
November 2018: Veterinary World
https://www.readbyqxmd.com/read/30529098/integrated-mirna-and-mrna-transcriptomes-of-spleen-profiles-between-yorkshire-and-queshan-black-pigs
#3
Xinjian Li, Ruimin Qiao, Jianwei Ye, Mingyu Wang, Chen Zhang, Gang Lv, Kejun Wang, Xiuling Li, Xuelei Han
Disease causes large economic losses to the pig industry worldwidely, immunity plays an important role in the process of resistance to disease. In the present study, to elucidate the molecular mechanisms underlying different levels of disease resistance, we obtained the miRNA and mRNA expression profiles from the spleens of three groups of sows, including 180-day-old Queshan Black (Q-F), 3-day-old Yorkshire (Y-N) and 180-day-old Yorkshire (Y-F) pigs. The results showed that 85 miRNAs and 5093 genes were differentially expressed in Y-F vs Y-N, and 20 miRNAs and 1283 genes were differentially expressed in Q-F vs Y-F...
December 7, 2018: Gene
https://www.readbyqxmd.com/read/30497374/investigation-of-allele-specific-expression-of-genes-involved-in-adipogenesis-and-lipid-metabolism-suggests-complex-regulatory-mechanisms-of-ppargc1a-expression-in-porcine-fat-tissues
#4
Monika Stachowiak, Izabela Szczerbal, Krzysztof Flisikowski
BACKGROUND: The expression of genes involved in regulating adipogenesis and lipid metabolism may affect economically important fatness traits in pigs. Allele-specific expression (ASE) reflects imbalance between allelic transcript levels and can be used to identify underlying cis-regulatory elements. ASE has not yet been intensively studied in pigs. The aim of this investigation was to analyze the differential allelic expression of four genes, PPARA, PPARG, SREBF1, and PPARGC1A, which are involved in the regulation of fat deposition in porcine subcutaneous and visceral fat and longissimus dorsi muscle...
November 29, 2018: BMC Genetics
https://www.readbyqxmd.com/read/30488155/disruption-of-the-zbed6-binding-site-in-intron-3-of-igf2-by-crispr-cas9-leads-to-enhanced-muscle-development-in-liang-guang-small-spotted-pigs
#5
Xiaofeng Liu, Hongbo Liu, Min Wang, Ruiqiang Li, Jianhua Zeng, Delin Mo, Peiqing Cong, Xiaohong Liu, Yaosheng Chen, Zuyong He
Insulin-like growth factor 2 (IGF2) plays an important role in the development of the foetus and in post-natal growth and development. A SNP within intron 3 of porcine IGF2 disrupts a binding site for the repressor, zinc finger BED-type containing 6 (ZBED6), leading to up-regulation of IGF2 in skeletal muscle and major effects on muscle growth, heart size, and fat deposition. This favourable mutation is common in Western commercial pig populations, but is not present in most indigenous Chinese pig breeds. Here, we described the efficient disruption of the ZBED6 binding site motif in intron 3 of IGF2 by CRISPR/Cas9 in porcine embryonic fibroblasts (PEFs) from the indigenous Chinese pig breed, Liang Guang Small Spotted pig...
November 28, 2018: Transgenic Research
https://www.readbyqxmd.com/read/30478374/hotspots-of-recent-hybridization-between-pigs-and-wild-boars-in-europe
#6
Laura Iacolina, Cino Pertoldi, Marcel Amills, Szilvia Kusza, Hendrik-Jan Megens, Valentin Adrian Bâlteanu, Jana Bakan, Vlatka Cubric-Curic, Ragne Oja, Urmas Saarma, Massimo Scandura, Nikica Šprem, Astrid Vik Stronen
After a strong demographic decline before World War II, wild boar populations are expanding and the species is now the second-most abundant ungulate in Europe. This increase raises concerns due to wild boar impact on crops and natural ecosystems and as potential vector of diseases. Additionally, wild boar can hybridize with domestic pigs, which could increase health risks and alter wild boar adaptive potential. We analysed 47,148 Single Nucleotide Polymorphisms in wild boar from Europe (292) and the Near East (16), and commercial (44) and local (255) pig breeds, to discern patterns of hybridization across Europe...
November 26, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30476737/colistin-resistance-prevalence-in-escherichia-coli-from-domestic-animals-in-intensive-breeding-farms-of-jiangsu-province
#7
X Zhang, B Zhang, Y Guo, J Wang, P Zhao, J Liu, K He
The global dissemination of colistin resistance has received a great deal of attention. Recently, the plasmid-mediated colistin resistance encoded by mcr genes in Escherichia coli (E. coli) strains from animals, food, and patients in China has been reported frequently. To investigate the colistin resistance and mcr-1 and mcr-2 genes spread in domestic animals in Jiangsu Province, we collected fecael swabs from pigs, chicken and cattle at different ages distributed in intensive feeding farms. The selective chromogenic agar and mcr-PCR were used to screen the colistin resistance and mcr gene carriage...
November 22, 2018: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/30473358/observations-on-the-distribution-and-persistence-of-monophasic-salmonella-typhimurium-on-infected-pig-and-cattle-farms
#8
Rebecca J Gosling, Doris Mueller-Doblies, Francesca Martelli, Javier Nunez-Garcia, Nick Kell, Andre Rabie, Andy D Wales, Robert H Davies
Following a rapid rise in cases of monophasic Salmonella Typhimurium DT193 (mST) in humans and pigs since 2007 a detailed study of the prevalence and persistence of mST on pig and cattle farms in Great Britain (GB) was undertaken. Thirteen commercial pig farms and twelve cattle farms, identified as mST-positive from surveillance data, were intensively sampled over a three year period. Five indoor and eight outdoor pig farms and four beef and eight dairy farms were included. Individual and pooled faecal samples were collected from each epidemiological group and environmental samples throughout each farm and the antimicrobial resistance profile determined for a selection of mST-positive isolates...
December 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/30473346/role-of-wild-birds-and-environmental-contamination-in-the-epidemiology-of-salmonella-infection-in-an-outdoor-pig-farm
#9
Alessia De Lucia, André Rabie, Richard P Smith, Rob Davies, Fabio Ostanello, Dolapo Ajayi, Liljana Petrovska, Francesca Martelli
Foodborne outbreaks caused by Salmonella are often attributed to the pork consumption. Salmonella contamination of retail pork is directly linked to the Salmonella prevalence on farm. In UK, approximately 40% of breeding pigs are kept outdoors. Aim of this study was to investigate the role of wild birds in the epidemiology of Salmonella in one outdoor pig farm. Three sampling visits were carried out at monthly intervals to an outdoor farm consisting of two fields, one left empty of pigs for more than 2 years (field A) while the second (field B) was occupied by pigs during the first visit only...
December 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/30466656/breed-to-wean-farm-factors-associated-with-influenza-a-virus-infection-in-piglets-at-weaning
#10
Fabian Orlando Chamba Pardo, Adam Schelkopf, Matthew Allerson, Robert Morrison, Marie Culhane, Andres Perez, Montserrat Torremorell
Breed-to-wean pig farms play an important role in spreading influenza A virus (IAV) because suckling piglets maintain, diversify and transmit IAV at weaning to other farms. Understanding the nature and extent of which farm factors drive IAV infection in piglets is a prerequisite to reduce the burden of influenza in swine. We evaluated the association between IAV infection in piglets at weaning and farm factors including farm features, herd management practices and gilt- and piglet-specific management procedures performed at the farm...
December 1, 2018: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/30458028/diversity-across-major-and-candidate-genes-in-european-local-pig-breeds
#11
María Muñoz, Riccardo Bozzi, Fabián García, Yolanda Núñez, Claudia Geraci, Alessandro Crovetti, Juan García-Casco, Estefania Alves, Martin Škrlep, Rui Charneca, Jose M Martins, Raquel Quintanilla, Joan Tibau, Goran Kušec, Ivona Djurkin-Kušec, Marie J Mercat, Juliette Riquet, Jordi Estellé, Christoph Zimmer, Violeta Razmaite, Jose P Araujo, Čedomir Radović, Radomir Savić, Danijel Karolyi, Maurizio Gallo, Marjeta Čandek-Potokar, Luca Fontanesi, Ana I Fernández, Cristina Óvilo
The aim of this work was to analyse the distribution of causal and candidate mutations associated to relevant productive traits in twenty local European pig breeds. Also, the potential of the SNP panel employed for elucidating the genetic structure and relationships among breeds was evaluated. Most relevant genes and mutations associated with pig morphological, productive, meat quality, reproductive and disease resistance traits were prioritized and analyzed in a maximum of 47 blood samples from each of the breeds (Alentejana, Apulo-Calabrese, Basque, Bísara, Majorcan Black, Black Slavonian (Crna slavonska), Casertana, Cinta Senese, Gascon, Iberian, Krškopolje (Krškopoljski), Lithuanian indigenous wattle, Lithuanian White Old Type, Mora Romagnola, Moravka, Nero Siciliano, Sarda, Schwäbisch-Hällisches Schwein (Swabian Hall pig), Swallow-Bellied Mangalitsa and Turopolje)...
2018: PloS One
https://www.readbyqxmd.com/read/30456690/impact-of-on-farm-feeding-practices-on-the-reproductive-indices-of-pigs-reared-under-educated-smallholder-system-in-imo-state-nigeria
#12
I C Okoli, C C Achonwa, I P Ogbuewu, C Ezema, C A Mbajiorgu, M C Emesowurum
The aim of this study was to determine the impact of on-site feeding practices on the reproductive indices of pigs reared under educated smallholder system in Imo State, southeastern Nigeria. The smallholder pig farms (S1, S2, M, L1, and L2) visited were divided into small-, medium-, and large-size farms, with small farms stocking 70-80, medium 120-130, and larger 230-270 pigs. In each farm, six weaner pigs comprising Large White, Landrace, and Duroc breeds were selected and their ages and initial body weights determined...
November 19, 2018: Tropical Animal Health and Production
https://www.readbyqxmd.com/read/30448370/serological-and-molecular-analysis-for-brucellosis-in-selected-swine-herds-from-southern-india
#13
Rajeswari Shome, Triveni Kalleshamurthy, Krithiga Natesan, K Rohit Jayaprakash, Kavya Byrareddy, Nagalingam Mohandoss, Swati Sahay, Bibek R Shome, Jagadish Hiremath, Habibur Rahman, Sukhadeo B Barbuddhe
BACKGROUND: Swine brucellosis is a zoonotic disease caused by Brucella suis. The study describes the occurrence of brucellosis in two organized piggeries in Southern India. METHODS: A total of 585 serum samples comprising 575 from pigs and 10 from animal handlers were collected and tested by serological tests and PCR. Tissue samples were collected for isolation of the pathogen. RESULTS: Out of 575 serum samples screened, 236 (41.04%) were positive for brucellosis by both Rose Bengal plate test (RBPT) and indirect ELISA (iELISA) and 47 (8...
November 15, 2018: Journal of Infection and Public Health
https://www.readbyqxmd.com/read/30439481/snps-associated-with-body-weight-and-backfat-thickness-in-two-pig-breeds-identified-by-a-genome-wide-association-study
#14
Qiang Yang, Pingxian Wu, Kai Wang, Dejuan Chen, Jie Zhou, Jideng Ma, Mingzhou Li, Weihang Xiao, Anan Jiang, Yanzhi Jiang, Lin Bai, Li Zhu, Xuewei Li, Guoqing Tang
Growth and fat deposition are important economic traits due to the influence on production in pigs. In this study, a dataset of 1200 pigs with 345,570 SNPs genotyped by sequencing (GBS) was used to conduct a GWAS with single-marker regression method to identify SNPs associated with body weight and backfat thickness (BFT) and to search for candidate genes in Landrace and Yorkshire pigs. A total of 27 and 13 significant SNPs were associated with body weight and BFT, respectively. In the region of 149.85-149.89 Mb on SSC6, the SNP (SSC6: 149876737) for body weight and the SNP (SSC6: 149876507) for BFT were in the same locus region (a gap of 230 bp)...
November 12, 2018: Genomics
https://www.readbyqxmd.com/read/30427860/sow-communication-with-piglets-while-being-active-is-a-good-predictor-of-maternal-skills-piglet-survival-and-litter-quality-in-three-different-breeds-of-domestic-pigs-sus-scrofa-domesticus
#15
Marko Ocepek, Inger Lise Andersen
Maternal care behaviour is crucial for offspring quality and survival in pigs. Defining care is therefore essential for ensuring the welfare of pigs and sustainability of pig production. The aim of the present study was to investigate the relationship between sow nest building, communication with piglets (sniffing, nudging, grunting) during resting and activity, and piglet survival in three different sow breeds: a maternal line selected for high weaned pig production (Landrace), a paternal line selected for meat traits (Duroc), and a crossbred line (Landrace and Yorkshire)...
2018: PloS One
https://www.readbyqxmd.com/read/30423578/genome-wide-analysis-of-mrnas-and-lncrnas-of-intramuscular-fat-related-to-lipid-metabolism-in-two-pig-breeds
#16
Wanlong Huang, Xiuxiu Zhang, Ai Li, Lingli Xie, Xiangyang Miao
BACKGROUND/AIMS: Long non-coding RNAs (lncRNAs) can regulate adipogenesis and lipid accumulation. Intramuscular fat deposition appears to vary in different pig breeds, and the regulation mechanism has not yet been fully elucidated at molecular level. Moreover, little is known about the function and profile of lncRNAs in intramuscular fat deposition and metabolism in pig. The aim of this study was thus to explore the regulatory functions of lncRNAs in intramuscular fat deposition. METHODS: In this study, Laiwu (LW) pig and Large White (LY) pig with significant difference in fat deposition were selected for use...
2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/30423111/genomic-prediction-for-crossbred-performance-using-metafounders
#17
E M van Grevenhof, J Vandenplas, M P L Calus
Future genomic evaluation models to be used routinely in breeding programs for pigs and poultry need to be able to optimally use information of crossbred animals to predict breeding values for crossbred performance of purebred selection candidates. Important challenges in the commonly used single-step genomic best linear unbiased prediction (ssGBLUP) model, are the definition of relationships between the different line compositions and the definition of the base generation per line. The use of metafounders (MFs) in ssGBLUP has been proposed to overcome these issues...
November 13, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/30418803/meeting-global-feed-protein-demand-challenge-opportunity-and-strategy
#18
Sung Woo Kim, John F Less, Li Wang, Tianhai Yan, Viswanath Kiron, Sadasivam J Kaushik, Xin Gen Lei
Feed protein supplements are one of the most expensive and limiting feed ingredients. This review offers a comprehensive analysis of how the expected expansion of animal production, driven by the rising world population and living standards for more animal-sourced foods, is creating a global shortage of feed protein supply. Because ruminants, chickens, and pigs contribute to 96% of the global supply of animal protein and aquaculture is growing fast, means of meeting the feed protein requirements of these species are elaborated...
November 12, 2018: Annual Review of Animal Biosciences
https://www.readbyqxmd.com/read/30417949/unravelling-the-genetic-loci-for-growth-and-carcass-traits-in-chinese-bamaxiang-pigs-based-on-a-1-4-million-snp-array
#19
Huanfa Gong, Shijun Xiao, Wanbo Li, Tao Huang, Xiaochang Huang, Guorong Yan, Yizhong Huang, Hengqing Qiu, Kai Jiang, Xiaopeng Wang, Hui Zhang, Jianhong Tang, Lin Li, Yiping Li, Chenbin Wang, Chuanmin Qiao, Jun Ren, Lusheng Huang, Bin Yang
Bamaxiang pig is from Guangxi province in China, characterized by its small body size and two-end black coat colour. It is an important indigenous breed for local pork market and excellent animal model for biomedical research. In this study, we performed genomewide association studies (GWAS) on 43 growth and carcass traits in 315 purebred Bamaxiang pigs based on a 1.4 million SNP array. We observed considerable phenotypic variability in the growth and carcass traits in the Bamaxiang pigs. The corresponding SNP based heritability varied greatly across the 43 traits and ranged from 9...
November 12, 2018: Journal of Animal Breeding and Genetics, Zeitschrift Für Tierzüchtung und Züchtungsbiologie
https://www.readbyqxmd.com/read/30405681/imputation-based-whole-genome-sequence-association-study-reveals-constant-and-novel-loci-for-hematological-traits-in-a-large-scale-swine-f-2-resource-population
#20
Guorong Yan, Tianfu Guo, Shijun Xiao, Feng Zhang, Wenshui Xin, Tao Huang, Wenwu Xu, Yiping Li, Zhiyan Zhang, Lusheng Huang
The whole-genome sequences of progenies with low-density single-nucleotide polymorphism (SNP) genotypes can be imputed with high accuracy based on the deep-coverage sequences of key ancestors. With this imputation technology, a more powerful genome-wide association study (GWAS) can be carried out using imputed whole-genome variants and the phenotypes of interest to overcome the shortcomings of low-power detection and the large confidence interval derived from low-density SNP markers in classic association studies...
2018: Frontiers in Genetics
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