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https://www.readbyqxmd.com/read/30339471/mtch2-promotes-adipogenesis-in-intramuscular-preadipocytes-via-an-m-6-a-ythdf1-dependent-mechanism
#1
Qin Jiang, Baofa Sun, Qing Liu, Min Cai, Ruifan Wu, Fengqin Wang, Yongxi Yao, Yizhen Wang, Xinxia Wang
Intramuscular fat is considered a potential factor that is associated with meat quality in animal production and insulin resistance in humans. N6 -methyladenosine (m6 A) modification of mRNA plays an important role in regulating adipogenesis. However, the effects of m6 A on the adipogenesis of intramuscular preadipocytes and associated mechanisms remain unknown. Here, we performed m6 A sequencing to compare m6 A methylome of the longissimus dorsi muscles (LDMs) between Landrace pigs (lean-type breed) and Jinhua pigs (obese-type breed with higher levels of intramuscular fat)...
October 19, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/30339027/comparative-analysis-of-long-noncoding-rnas-expressed-during-intramuscular-adipocytes-adipogenesis-in-fat-type-and-lean-type-pigs
#2
Yunmei Sun, Xiaochang Chen, Jin Qin, Shuge Liu, Rui Zhao, Taiyong Yu, Guiyan Chu, Gongshe Yang, Pang Wei-Jun
The meat quality of local breed pigs is more tender and juicier than the imported varieties. The important reason is that the intramuscular fat content is high. Even though modest sequence conservation and evolution, the expression pattern and function of long non-coding RNAs (lncRNAs) seem to be conserved. In spite of that, analysis of lncRNAs associated with intramuscular fat development remains unknown to us in porcine. Here, we systematically investigated lncRNAs of intramuscular adipocytes of fat local Bamei pig and lean Large White pig to consider the function of lncRNAs on intramuscular fat development...
October 19, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/30336733/comparative-metabolomics-of-japanese-black-cattle-beef-and-other-meats-using-gas-chromatography-mass-spectrometry
#3
Shuji Ueda, Eiji Iwamoto, Yoshiki Kato, Masakazu Shinohara, Yasuhito Shirai, Minoru Yamanoue
Progress in metabolomic analysis now allows the evaluation of food quality. This study aims to identify the metabolites in meat from livestock using a metabolomic approach. Using gas chromatography-mass spectrometry (GC/MS), many metabolites were reproducibly detected in meats, and distinct differences between livestock species (cattle, pigs, and chickens) were indicated. A comparison of metabolites between tissues types (muscle, intramuscular fat, and intermuscular fat) in marbled beef of Japanese Black cattle revealed that most metabolites are abundant in the muscle tissue...
October 18, 2018: Bioscience, Biotechnology, and Biochemistry
https://www.readbyqxmd.com/read/30333655/classifying-different-iberian-pig-genetic-lines-by-applying-chemical-instrumental-parameters-of-dry-cured-iberian-shoulders
#4
D Caballero, M Asensio, C Fernández, N Martín, A Silva
Iberian pigs are an autochthonous porcine breed mainly bred in southwestern of Iberian Peninsula, this herd traditionally feeds on acorn and pasture, in an extensive production system. In this study, the main objective was for classifying Iberian shoulders from different genetic lines of pigs and their crossed genetic lines. For that, morphology parameters, fatty acid profile and volatile compounds were determined on two batches of dry-cured Iberian shoulders. The samples were acquired from the same place of the dry-cured Iberian shoulders...
November 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/30326713/comparison-of-the-histology-of-the-skin-of-the-windsnyer-kolbroek-and-large-white-pigs
#5
Davison Moyo, Monica Gomes, Kennedy H Erlwanger
The skin is a protective barrier, and an endocrine, sensory and thermoregulatory organ. We investigated whether the skin of local pigs had beneficial anatomical traits compared to exotic pigs to withstand the increased heat loads predicted under future climate change scenarios. Full-thickness skin specimens were obtained from the dorsal interscapular, lateral thoraco-abdominal and ventral abdominal regions of intact boars (age 6-8 months) of two local breeds of pigs (Windsnyer [n = 5] and Kolbroek [n = 4]) and an exotic pig breed (Large White [n = 7])...
September 25, 2018: Journal of the South African Veterinary Association
https://www.readbyqxmd.com/read/30326643/breed-differences-in-pcv2-uptake-and-disintegration-in-porcine-monocytes
#6
Ruifang Wei, Ivan Trus, Bo Yang, Liping Huang, Hans J Nauwynck
Porcine circovirus type 2 (PCV2) is associated with various diseases which are designated as PCV2-associated diseases (PCVADs). Their severity varies among breeds. In the diseased pigs, virus is present in monocytes, without replication or full degradation. PCV2 entry and viral outcome in primary porcine monocytes and the role of monocytes in PCV2 genetic susceptibility have not been studied. Here, virus uptake and trafficking were analyzed and compared among purebreds Piétrain, Landrace and Large White and hybrid Piétrain × Topigs20...
October 15, 2018: Viruses
https://www.readbyqxmd.com/read/30324759/distribution-of-runs-of-homozygosity-in-chinese-and-western-pig-breeds-evaluated-by-reduced-representation-sequencing-data
#7
Zhe Zhang, Qianqian Zhang, Qian Xiao, Hao Sun, Hongding Gao, Yumei Yang, Jiucheng Chen, Zhengcao Li, Ming Xue, Peipei Ma, Hongjie Yang, Ningying Xu, Qishan Wang, Yuchun Pan
Inbreeding, which has several causes including genetic drift, population bottlenecks, mating of close relatives and selection, can leave tracts of runs of homozygosity (ROH) along genomes. Recently, decreasing performance on reproductive traits, which might have resulted from inbreeding, has been observed in Chinese pigs. In this study, 830 individuals from Western and Chinese pig breeds were genotyped using the reduced-representation sequencing method. After imputation and quality control, 60 850 high-confidence SNPs were retained for ROH detection...
October 16, 2018: Animal Genetics
https://www.readbyqxmd.com/read/30322538/evolutionary-dynamics-of-classical-swine-fever-virus-in-south-korea-1987-2017
#8
Dong-Jun An, Seong-In Lim, SeEun Choe, Ki-Sun Kim, Ra Mi Cha, In-Soo Cho, Jae-Young Song, Bang-Hun Hyun, Bong-Kyun Park
The 5' UTR (n=102) and full-length E2 (n=37) genes of classical swine fever viruses (CSFVs) circulating in South Korea over the past 30 years (1987-2017) were examined to determine the evolutionary rate and estimated time of the most recent common ancestor (tMRCA). From 2000, the Korean classical swine fever (CSF) antigen changed from genotype 3 to 2, which comprises subgenotypes 2.1b (2002-2013) and 2.1d (2011-2017). There are genotypic variations in the full-length E2 gene of Korean CSFV genotypes 2.1b and 2...
November 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/30321346/response-to-a-selection-index-including-environmental-costs-and-risk-preferences-of-producers
#9
Beshir M Ali, John W M Bastiaansen, Yann de Mey, Alfons G J M Oude Lansink
Genetic improvement of animals plays an important role in improving the economic and environmental sustainability of livestock production systems. This paper proposes a method to incorporate mitigation of environmental impacts and risk preferences of producers into a breeding objective via economic values (EVs). The paper assesses the effects of using these alternative EVs of breeding goal traits on discounted economic response to selection and on environmental impacts at commercial farm level. The application focuses on a Brazilian pig production system...
October 15, 2018: Journal of Animal Science
https://www.readbyqxmd.com/read/30314790/epidemiology-of-pseudorabies-in-intensive-pig-farms-in-shanghai-china-herd-level-prevalence-and-risk-factors
#10
Luming Xia, Quanyun Sun, Jingjing Wang, Qi Chen, Peihong Liu, Chaojian Shen, Jianhe Sun, Yiping Tu, Sufang Shen, Jiuchao Zhu, Hongjin Zhao, Quzhi Wang, Baolong Li, Jun Tao, Ricardo J Soares Magalhaes, Yaxian Yan, Chang Cai
Pseudorabies (PR), also known as Aujeszky's disease, is a highly contagious disease affecting pigs and a wide range of animals. Pseudorabies is enzootic in many countries. In China, it is a priority animal disease for control and eradication, however the data on disease frequency in intensive pig farms and the information on associated risk factors is inadequate. A cross-sectional study of intensive pig farms (≥350 sows) in Shanghai was conducted to determine herd-level prevalence of PRV and associated risk factors...
November 1, 2018: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/30314772/factors-associated-with-specific-health-welfare-and-reproductive-performance-indicators-in-pig-herds-from-five-eu-countries
#11
Ilias Chantziaras, Jeroen Dewulf, Tommy Van Limbergen, Marlijn Klinkenberg, Andreas Palzer, Carlos Pineiro, Vivi Aarestrup Moustsen, Jarkko Niemi, Ilias Kyriazakis, Dominiek Maes
Production diseases are often of multi-factorial origin in which environment (housing, nutrition and management) health and reproductive challenges show complex interactions. The aim of this study was to identify specific environment-related factors and to discuss their associations with health, welfare and reproductive performance in sows and piglets, in diverse systems using data from 130 farms from five EU countries. Two sets of data were used: a) a questionnaire was developed for sows and piglets covering farm management, interventions and housing and b) farm production data covering various performance parameters...
November 1, 2018: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/30312908/development-and-evaluation-of-diffusive-gradients-in-thin-films-based-on-nano-sized-zinc-oxide-particles-for-the-in-situ-sampling-of-tetracyclines-in-pig-breeding-wastewater
#12
Nan You, Hui Yao, Yun Wang, Hong-Tao Fan, Chang-Song Wang, Ting Sun
The pollution of antibiotics, including tetracyclines (TCs), in aquatic environments has become an issue of concern in recent years. Herein, an in situ sampling of TCs in pig breeding wastewater that utilizes the technique of diffusive gradients in thin films (DGT), based on commercial nanosized ZnO (nanoZnO) particles as the potential effective binding agent and a polyethersulfone (PES) membrane as the diffusion layer, was developed. The diffusion coefficients of tetracycline (TC), oxytetracycline (OTC) and chlortetracycline (CTC) in a PES membrane at 25 °C were (1...
September 25, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/30309204/genome-wide-association-studies-associated-with-backfat-thickness-in-landrace-and-yorkshire-pigs
#13
Young-Sup Lee, Donghyun Shin
Although pork quality traits are important commercially, genome-wide association studies (GWASs) have not well considered Landrace and Yorkshire pigs worldwide. Landrace and Yorkshire pigs are important pork-providing breeds. Although quantitative trait loci of pigs are well-developed, significant genes in GWASs of pigs in Korea must be studied. Through a GWAS using the PLINK program, study of the significant genes in Korean pigs was performed. We conducted a GWAS and surveyed the gene ontology (GO) terms associated with the backfat thickness (BF) trait of these pigs...
September 2018: Genomics & Informatics
https://www.readbyqxmd.com/read/30308950/apulo-calabrese-and-crossbreed-pigs-show-different-physiological-response-and-meat-quality-traits-after-short-distance-transport
#14
Gizella Aboagye, Stefania Dall'Olio, Francesco Tassone, Martina Zappaterra, Salvatore Carpino, Leonardo Nanni Costa
Despite the increasing interest in the welfare of animals during transport, very little is known on the response of local pig breeds to the transport procedures. This study aims to compare the effect of short journey on behaviour, blood parameters, and meat quality traits in 51 Apulo-Calabrese and 52 crossbreed [Duroc × (Landrace × Large White)] pigs. All the animals were blood sampled five days before delivery (basal condition) and at exsanguination for the analysis of creatine kinase, cortisol, glucose, lactate, albumin, albumin/globulin, total protein, urea, creatinine, aspartate aminotransferase (AST), alanine aminotransferase, alkaline phosphate, sodium, and potassium...
October 10, 2018: Animals: An Open Access Journal From MDPI
https://www.readbyqxmd.com/read/30308042/genome-wide-association-studies-and-meta-analysis-uncovers-new-candidate-genes-for-growth-and-carcass-traits-in-pigs
#15
Iulia Blaj, Jens Tetens, Siegfried Preuß, Jörn Bennewitz, Georg Thaller
Genome-wide association studies (GWAS) have been widely used in the genetic dissection of complex traits. As more genomic data is being generated within different commercial or resource pig populations, the challenge which arises is how to collectively investigate the data with the purpose to increase sample size and implicitly the statistical power. This study performs an individual population GWAS, a joint population GWAS and a meta-analysis in three pig F2 populations. D1 is derived from European type breeds (Piétrain, Large White and Landrace), D2 is obtained from an Asian breed (Meishan) and Piétrain, and D3 stems from a European Wild Boar and Piétrain, which is the common founder breed...
2018: PloS One
https://www.readbyqxmd.com/read/30305017/effects-of-alleles-in-crossbred-pigs-estimated-for-genomic-prediction-depend-on-their-breed-of-origin
#16
Claudia A Sevillano, Jan Ten Napel, Simone E F Guimarães, Fabyano F Silva, Mario P L Calus
BACKGROUND: This study investigated if the allele effect of a given single nucleotide polymorphism (SNP) for crossbred performance in pigs estimated in a genomic prediction model differs depending on its breed-of-origin, and how these are related to estimated effects for purebred performance. RESULTS: SNP-allele substitution effects were estimated for a commonly used SNP panel using a genomic best linear unbiased prediction model with breed-specific partial relationship matrices...
October 11, 2018: BMC Genomics
https://www.readbyqxmd.com/read/30302803/a-comparison-of-prenatal-muscle-transcriptome-and-proteome-profiles-between-pigs-with-divergent-growth-phenotypes
#17
Peng Shang, Zhixiu Wang, Yangzom Chamba, Bo Zhang, Hao Zhang, Changxin Wu
The growth of pigs is an important economic trait that involves multiple genes and coordinated regulatory mechanisms. The growth rate and potential of skeletal muscles are largely decided by embryonic myofiber development. Tibetan pig (TP) that is a mini-type breed has a divergent phenotype in growth rate and adult body weight with Wujin pig (WJ) and large White pig (LW). In the current study, the transcriptome (using RNA-seq) and proteome (using the isobaric tag for relative and absolute quantification [iTRAQ]) data from the prenatal muscle tissues were analyzed to identify the genes related to postnatal growth rate and growth potential in pigs...
October 9, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/30298349/a-haplotype-variant-of-porcine-ifit2-increases-poly-i-c-induced-activation-of-nf-%C3%AE%C2%BAb-and-isre-binding-factors
#18
Yu Pang, Caixia Zhang, Yaguang Tian, Yanfang Song, Di Liu, Xiuqin Yang
Interferon-induced protein with tetratricopeptide repeats (IFIT) 2 is associated with various viral infections and pathogenesis in humans and mice. However, there are few reports on IFIT2 in pigs and the polymorphic information remains unclear. Here, by using a direct PCR sequencing method, we identified four single nucleotide polymorphisms (SNPs), c.259G>A (p.Gly87Ser), c.520T>G (p.Phe174Val), c.571C>T (p.Pro191Ser), and c.879A>G (p.Glu293Glu), for the first time in the coding sequence of the porcine (p) IFIT2 gene from a Chinese local breed (Hebao pig), Western commercial pig breeds (Yorkshire and Landrace), and a Chinese developed breed (Beijing Black pig)...
October 8, 2018: Molecular Biology Reports
https://www.readbyqxmd.com/read/30293648/use-of-processing-fluids-and-serum-samples-to-characterize-porcine-reproductive-and-respiratory-syndrome-virus-dynamics-in-3-day-old-pigs
#19
Carles Vilalta, Juan Sanhueza, Julio Alvarez, Deb Murray, Montserrat Torremorell, Cesar Corzo, Robert Morrison
Collection of serum samples of pigs at weaning to monitor for porcine reproductive and respiratory syndrome virus (PRRSV) has become a common practice to determine PRRSV herd infection status. Diagnostic sensitivity of this practice is low in herds undergoing PRRSV elimination once prevalence of infection is near zero. Thus, the goal of this study was to characterize the dynamics of PRRSV infection in 3 day-old pigs overtime using serum and serosanguineous fluids obtained as part of castration and tail docking practices (processing fluids (PF))...
November 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/30292698/a-ct-image-based-pig-atlas-model-and-its-potential-applications-in-the-meat-industry
#20
H Ho, H B Yu, L E Gangsei, J Kongsro
In this communication we present a novel pig atlas model which is represented by a parametric linear Lagrange or cubic Hermite mesh. The model is developed from data points digitized from a 3D pig CT image. In total 84 muscles and 121 bones are included in the atlas, representing the tissue structures most relevant to the industry. We discuss its potential applications in virtual meat cuts and statistical shape analysis for pig breeding and genetics companies.
September 21, 2018: Meat Science
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