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epithelial to mesenchymal transition

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https://www.readbyqxmd.com/read/28109909/erk2-zeb1-mir-101-1-axis-contributes-to-epithelial-mesenchymal-transition-and-cell-migration-in-cancer
#1
Kailash Chandra Mangalhara, Siddharth Manvati, Sunil Kumar Saini, Kalaiarasan Ponnusamy, Gaurav Agarwal, Suresh K Abraham, Rameshwar N K Bamezai
Regulation of metastasis continues to remain enigmatic despite our improved understanding of cancer. Identification of microRNAs associated with metastasis in the recent past has provided a new hope. Here, we show how microRNA-101 (miR-101) regulates two independent processes of cellular metastasis by targeting pro-metastatic upstream regulatory transcription factors, ZEB1 and ZEB2, and downstream effector-actin modulators, RHOA and RAC1, providing a single target for therapeutic intervention. Further, we depict how down-regulation of miR-101 by extracellular signal-regulated kinase-2 (ERK2) is vital for MAP kinase pathway induced cellular migration and mesenchymal transition...
January 18, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28109697/fibronectin-fibrils-regulate-tgf-%C3%AE-1-induced-epithelial-mesenchymal-transition
#2
Lauren A Griggs, Nadiah T Hassan, Roshni S Malik, Brian Griffin, Brittany A Martinez, Lynne W Elmore, Christopher A Lemmon
Epithelial-Mesenchymal Transition (EMT) is a dynamic process through which epithelial cells transdifferentiate from an epithelial phenotype into a mesenchymal phenotype. Previous studies have also demonstrated that both mechanical signaling and soluble growth factor signaling facilitate this process. One possible point of integration for mechanical and growth factor signaling is the extracellular matrix. Here we investigate the role of the extracellular matrix (ECM) protein fibronectin (FN) in this process...
January 18, 2017: Matrix Biology: Journal of the International Society for Matrix Biology
https://www.readbyqxmd.com/read/28109152/-hepatic-stellate-cell-conditioned-medium-induces-proliferation-and-epithelial-mesenchymal-transition-via-activating-erk1-2-signaling-pathway-in-hepatoma-cells
#3
Yuxiao Xie, Rui Liao, Long Pan, Kai Fan, Cong Peng, Chengyou Du
Objective To investigate the influence of hepatic stellate cells (HSCs) on malignant biological behavior of hepatoma cells and related mechanisms. Methods Human hepatoma cell lines SMMC-7721 and HepG2, and hepatic stellate cell line LX-2 were cultured separately. HSC conditioned medium (LX2-CM), MAPK specific inhibitor U0126 were used to treat hepatoma cells, separately or together. The invasion and migration abilities of hepatoma cells were detected by Transwell(TM) assay, and cell proliferation was analyzed by CCK-8 assay...
February 2017: Xi Bao Yu Fen Zi Mian Yi Xue za Zhi, Chinese Journal of Cellular and Molecular Immunology
https://www.readbyqxmd.com/read/28109078/3-phosphoinositide-dependent-protein-kinase-1-pdk-1-promotes-migration-and-invasion-in-gastric-cancer-cells-through-activating-the-nf-%C3%AE%C2%BAb-pathway
#4
Ning Wu, Changyu He, Bohui Zhu, Jinling Jiang, Yiwen Chen, Tao Ma
Gastric cancer (GC) is one of the most common cancers and the second leading cause of cancer deaths in the world. Manyfactors were reported involving the progression and development of GC. In this study, we aimed to investigate the correlation of 3-phosphoinositide dependent protein kinase 1 (PDK-1) with cell viability, cell migration, and cell invasion of GC. The expression of PDK-1 was measured in different GC cell lines. Thereafter, the expression of PDK-1 was interfered by small hairpin RNA (shRNA) and then incubated with or without the inhibitor of NF-κB pyrrolidine dithiocarbamate (PDTC)...
January 20, 2017: Oncology Research
https://www.readbyqxmd.com/read/28108626/a-new-role-for-er%C3%AE-silencing-via-dna-methylation-of-basal-stem-cell-and-emt-genes
#5
Eric A Ariazi, John C Taylor, Michael A Black, Emmanuelle Nicolas, Michael J Slifker, Diana J Azzam, Jeff Boyd
: Resistance to hormonal therapies is a major clinical problem in the treatment of estrogen receptor α-positive (ERα(+)) breast cancers. Epigenetic marks, namely DNA methylation of cytosine at specific CpG sites (5mCpG), are frequently associated with ERα(+) status in human breast cancers. Therefore, ERα may regulate gene expression in part via DNA methylation. This hypothesis was evaluated using a panel of breast cancer cell line models of antiestrogen resistance. Microarray gene expression profiling was used to identify genes normally silenced in ERα(+) cells but derepressed upon exposure to the demethylating agent decitabine, derepressed upon long-term loss of ERα expression, and resuppressed by gain of ERα activity/expression...
November 15, 2016: Molecular Cancer Research: MCR
https://www.readbyqxmd.com/read/28108476/dynamic-analysis-of-the-mesenchymal-epithelial-transition-of-blood-brain-barrier-forming-glia-in-drosophila
#6
Tina Schwabe, Xiaoling Li, Ulrike Gaul
During development, many epithelia are formed by a mesenchymal-epithelial transition (MET). Here, we examine the major stages and underlying mechanisms of MET during blood-brain barrier formation in Drosophila We show that contact with the basal lamina is essential for the growth of the barrier-forming subperineurial glia (SPG). Septate junctions (SJs), which provide insulation of the paracellular space, are not required for MET, but are necessary for the establishment of polarized SPG membrane compartments...
January 20, 2017: Biology Open
https://www.readbyqxmd.com/read/28108315/essential-role-of-notch4-stat3-signaling-in-epithelial-mesenchymal-transition-of-tamoxifen-resistant-human-breast-cancer
#7
Quyen Thu Bui, Ji Hye Im, Sung Baek Jeong, Young-Mi Kim, Sung Chul Lim, Bumseok Kim, Keon Wook Kang
We previously demonstrated that tamoxifen (TAM)-resistant human breast cancer (TAMR-MCF-7) cells showed increased expression of mesenchymal marker proteins compared to the parent MCF-7 cells. Notch is functionally important in the promotion of epithelial-mesenchymal transition (EMT) during both development and tumor progression. Notch1 and Notch4 have been reported as prognostic markers in human breast cancer. Here, we indicated that Notch4, but not Notch1, plays a critical role in the regulation of EMT signaling in TAMR-MCF-7 cells...
January 17, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28108288/klf4-expression-enhances-the-efficacy-of-chemotherapy-drugs-in-ovarian-cancer-cells
#8
Baojin Wang, Airong Shen, Xuan Ouyang, Guannan Zhao, Ziyun Du, Wenying Huo, Tao Zhang, Yinan Wang, Chuanhe Yang, Peixin Dong, Hidemichi Watari, Lawrence M Pfeffer, Junming Yue
KLF4 is a transcriptional factor that can function either as a tumor suppressor or oncogene in cancer based on its cellular context. We recently demonstrated that KLF4 was a tumor suppressor in ovarian cancer cells by inhibiting the epithelial to mesenchymal transition. Here we report that KLF4 expression was downregulated in ovarian cancer tissue compared to normal ovarian tissue, and low KLF4 expression correlated with high risk ovarian carcinoma and poor patient survival. Enforced KLF4 expression by lentiviral transduction sensitized ovarian cancer cells to the effects of the chemotherapy drugs, paclitaxel and cisplatin...
January 17, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28107418/nf-kappab-is-involved-in-the-regulation-of-emt-genes-in-breast-cancer-cells
#9
Bruno R B Pires, Andre L Mencalha, Gerson M Ferreira, Waldemir F de Souza, José A Morgado-Díaz, Amanda M Maia, Stephany Corrêa, Eliana S F W Abdelhay
The metastatic process in breast cancer is related to the expression of the epithelial-to-mesenchymal transition transcription factors (EMT-TFs) SNAIL, SLUG, SIP1 and TWIST1. EMT-TFs and nuclear factor-κB (NF-κB) activation have been associated with aggressiveness and metastatic potential in carcinomas. Here, we sought to examine the role of NF-κB in the aggressive properties and regulation of EMT-TFs in human breast cancer cells. Blocking NF-κB/p65 activity by reducing its transcript and protein levels (through siRNA-strategy and dehydroxymethylepoxyquinomicin [DHMEQ] treatment) in the aggressive MDA-MB-231 and HCC-1954 cell lines resulted in decreased invasiveness and migration, a downregulation of SLUG, SIP1, TWIST1, MMP11 and N-cadherin transcripts and an upregulation of E-cadherin transcripts...
2017: PloS One
https://www.readbyqxmd.com/read/28107197/mtdh-promotes-glioma-invasion-through-regulating-mir-130b-cernas
#10
Liping Tong, Ming Chu, Bingqing Yan, Weiyi Zhao, Shuang Liu, Wei Wei, Huihuang Lou, Shengkun Zhang, Shuai Ma, Juan Xu, Lanlan Wei
Cell invasion is crucial for high mortality and recurrence rate in glioma. Epithelial-mesenchymal transition (EMT) is an important step in cancer invasion. Metadherin (MTDH) contributes to EMT in several cancers, but the role and mechanism of MTDH in EMT-like process of glioma remain unknown. Here we demonstrate that MTDH was overexpressed in glioma tissues and cells and induced EMT-like change and invasion of glioma cells. Interestingly, MTDH could modulate the expression of a group of glioma-related miRNAs...
January 18, 2017: Oncotarget
https://www.readbyqxmd.com/read/28107095/chibby1-knockdown-promotes-mesenchymal-to-epithelial-transition-like-changes
#11
Victoria Fischer, Michael Wong, Feng-Qian Li, Ken-Ichi Takemaru
Chibby1 (Cby1) was originally isolated as a binding partner for β-catenin, a dual function protein in cell-cell adhesion and in canonical Wnt signaling. The canonical Wnt/β-catenin pathway is dysregulated in various diseases including cancer, most notably of the gastrointestinal origin. To investigate the role of Cby1 in colorectal tumorigenesis, we generated stable Cby1-knockdown (KD) SW480 colon cancer cells. Unexpectedly, we found that Cby1 KD induces mesenchymal-to-epithelial transition (MET)-like changes in SW480 as well as in HEK293 cells...
January 20, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28106823/integrated-microrna-mrna-profiling-identifies-oncostatin-m-as-a-marker-of-mesenchymal-like-er-negative-her2-negative-breast-cancer
#12
Giulia Bottai, Lixia Diao, Keith A Baggerly, Laura Paladini, Balázs Győrffy, Carlotta Raschioni, Lajos Pusztai, George A Calin, Libero Santarpia
MicroRNAs (miRNAs) simultaneously modulate different oncogenic networks, establishing a dynamic system of gene expression and pathway regulation. In this study, we analyzed global miRNA and messenger RNA (mRNA) expression profiles of 17 cell lines representing different molecular breast cancer subtypes. Spearman's rank correlation test was used to evaluate the correlation between miRNA and mRNA expression. Hierarchical clustering and pathway analysis were also performed. Publicly available gene expression profiles (n = 699) and tumor tissues (n = 80) were analyzed to assess the relevance of key miRNA-regulated pathways in human breast cancer...
January 19, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28106491/effect-of-an-mg132-sustained-drug-delivery-capsular-ring-on-the-inhibition-of-posterior-capsule-opacification-in-a-rabbit-model
#13
Xuan Bao, Min Hou, Yingyan Qin, Furong Luo, Fu Shang, Mingxing Wu
PURPOSE: To design an MG132-sustained drug delivery capsular ring (SDDCR) and investigate its effect on the inhibition of posterior capsule opacification (PCO) in a rabbit model. METHODS: The SDDCRs were prepared by forming a slice of film made by the mixture of poly lactic-co-glycolic acid (PLGA) and MG132 on the surface of capsular tension rings (CTRs). The drug-loading capacity, entrapment efficiency, and in vitro release of the drug-containing film were detected...
January 20, 2017: Journal of Ocular Pharmacology and Therapeutics
https://www.readbyqxmd.com/read/28105226/expression-of-epidermal-growth-factor-like-domain-7-may-be-a-predictive-marker-of-the-effect-of-neoadjuvant-chemotherapy-for-locally-advanced-uterine-cervical-cancer
#14
Makoto Yamauchi, Takeshi Fukuda, Takuma Wada, Masaru Kawanishi, Kenji Imai, Reiko Tasaka, Tomoyo Yasui, Toshiyuki Sumi
Neoadjuvant chemotherapy (NAC) followed by hysterectomy may be effective for the treatment of locally advanced uterine cervical cancer and improve patient prognosis. It is important to identify markers that are able to predict whether NAC may be successful. Epidermal growth factor-like domain 7 (EGFL7) regulates vascular sprouting in blood vessel formation. In numerous types of human cancer, EGFL7 is upregulated and inhibits endothelial cell adhesion molecules, decreasing vascular tightness and, thus, increasing vascular permeability...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28105212/emt-influences-the-expression-of-ck19-in-pleural-effusion-derived-lung-cancer-cells-and-their-invasion-and-metastasis
#15
Dongyun Huang, Wenjing Xu, Xingxiang Xu, Xudong Zhang, Rui Zhou, Ping Chen
Lung cancer is the most common cancer after breast and colon cancer, with high rates of mortality, worldwide. There are two main types of lung cancer, small cell lung carcinoma (SCLC), which accounts for approximately 20% of all lung cancer cases and non-SCLC, which accounts for almost 80% of lung cancer cases. Although lung cancer is one of the most aggressive types of cancer, progress in achieving better clinical outcomes has been gradual. Even though a number of markers have been suggested for the diagnosis of lung cancer and monitoring of disease progression, there is no clear way of assessing the invasion, epithelial-mesenchymal transition (EMT) and metastasizing capability of the primary tumor cells...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28105207/cisplatin-promotes-mesenchymal-like-characteristics-in-osteosarcoma-through-snail
#16
Shuo Fang, Ling Yu, Hongjun Mei, Jian Yang, Tian Gao, Anyuan Cheng, Weichun Guo, Kezhou Xia, Gaiwei Liu
More than 30% of patients with osteosarcoma succumb to pulmonary metastases. Epithelial-mesenchymal transition (EMT) is a biological process by which tumor cells gain an increased capacity for invasiveness and metastasis. A previous study confirmed the phenomenon of EMT in osteosarcoma, a mesenchymal-derived tumor. However, whether chemotherapy affects EMT remains to be elucidated. In the present study, the osteosarcoma cells were exposed to a sublethal dose of cisplatin, and any surviving cells were assumed to be more resistant to cisplatin...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28105194/biomarkers-for-emt-and-met-in-breast-cancer-an-update
#17
Fei Liu, Li-Na Gu, Bao-En Shan, Cui-Zhi Geng, Mei-Xiang Sang
Metastasis and recurrence are the leading cause of mortality due to breast cancer, but the underlying mechanisms are still poorly understood. Understanding the breast cancer metastasis mechanism is important for early diagnosis and treatment of breast cancer. The seeding and growth of breast cancer cells at sites distinct from the primary tumor is a complex and multistage process. Recently, it has been reported that the epithelial-mesenchymal transition (EMT) and the mesenchymal-epithelial transition (MET) are the main mechanisms for breast cancer metastasis...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28104986/expression-of-trefoil-factors-and-twist1-in-colorectal-cancer-and-their-correlation-with-metastatic-potential-and-prognosis
#18
Akram Yusup, Bailikezi Huji, Cheng Fang, Fei Wang, Tuerxunjiang Dadihan, Hai-Jiang Wang, Halmurat Upur
AIM: To detect the expression of trefoil factors (TFFs) and TWIST1 in colorectal cancer (CRC) and analyze their correlation with metastasis and survival. METHODS: This study examined the expression of TFF1, TFF3 and TWIST1 in a total of 75 tumor samples, 47 matched normal samples (15 cm from the lesion margin), 30 metastatic lymph nodes, and 10 liver metastatic cancer samples from patients with CRC. The relationship was then analyzed between the protein expression and different clinical records...
January 7, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/28104398/down-regulation-of-boris-ctcfl-efficiently-regulates-cancer-stemness-and-metastasis-in-mycn-amplified-neuroblastoma-cell-line-by-modulating-wnt-%C3%AE-catenin-signaling-pathway
#19
Koteswara Rao Garikapati, Nibedita Patel, Venkata Krishna Kanth Makani, Priyanka Cilamkoti, Utpal Bhadra, Manika Pal Bhadra
BORIS/CTCFL is a vital nucleotide binding protein expressed during embryogenesis and gametogenesis. BORIS/CTCFL is the paralogue of transcriptional repressor protein CTCF, which is aberrantly expressed in various malignancies and primarily re-expressed in cancer stem cells (CSCs). The mechanism behind regulation of BORIS in various cancer conditions and tumor metastases is so far not explored in detail. The aim of the study was to understand the influence of BORIS/CTCFL on stemness and metastasis by regulating well-known oncogenes and related signaling pathways...
January 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28103577/association-of-rab5-overexpression-in-pancreatic-cancer-with-cancer-progression-and-poor-prognosis-via-e-cadherin-suppression
#20
Takamichi Igarashi, Kenichiro Araki, Takehiko Yokobori, Bolag Altan, Takahiro Yamanaka, Norihiro Ishii, Mariko Tsukagoshi, Akira Watanabe, Norio Kubo, Tadashi Handa, Yasuo Hosouchi, Masahiko Nishiyama, Tetsunari Oyama, Ken Shirabe, Hiroyuki Kuwano
Pancreatic cancer is a common type of cancer with poor prognosis worldwide. Postoperative survival depends on the existence of metastasis. Elucidation of the mechanism underlying cancer progression is important to improve prognosis. The RAS-associated protein RAB5 activates intracellular membrane trafficking, and RAB5 expression is correlated to progression and epithelial mesenchymal transition in various cancers.The expression of RAB5 and E-cadherin in 111 pancreatic cancer samples was investigated by immunohistochemical staining, and the relationship among RAB5 expression, clinicopathological factors, and E-cadherin expression was assessed...
January 17, 2017: Oncotarget
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