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epithelial to mesenchymal transition

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https://www.readbyqxmd.com/read/28545079/interleukin-like-emt-inducer-regulates-partial-phenotype-switching-in-mitf-low-melanoma-cell-lines
#1
Ken Noguchi, Annamarie C Dalton, Breege V Howley, Buckley J McCall, Akihiro Yoshida, J Alan Diehl, Philip H Howe
ILEI (FAM3C) is a secreted factor that contributes to the epithelial-to-mesenchymal transition (EMT), a cell biological process that confers metastatic properties to a tumor cell. Initially, we found that ILEI mRNA is highly expressed in melanoma metastases but not in primary tumors, suggesting that ILEI contributes to the malignant properties of melanoma. While melanoma is not an epithelial cell-derived tumor and does not undergo a traditional EMT, melanoma undergoes a similar process known as phenotype switching in which high (micropthalmia-related transcription factor) MITF expressing (MITF-high) proliferative cells switch to a low expressing (MITF-low) invasive state...
2017: PloS One
https://www.readbyqxmd.com/read/28545036/the-long-non-coding-rna-neat1-enhances-epithelial-to-mesenchymal-transition-and-chemoresistance-via-the-mir-34a-c-met-axis-in-renal-cell-carcinoma
#2
Fei Liu, Na Chen, Yanchun Gong, Ruihai Xiao, Weichao Wang, Zhengyue Pan
Long non-coding RNAs (lncRNAs) have emerged as new gene regulators and prognostic markers in various cancers. Although the lncRNA nuclear enriched abundant transcript 1 (NEAT1) has been associated with tumorigenesis, its functions in renal cell carcinoma (RCC) have not been elucidated. We determined that NEAT1 is up-regulated in RCC tissue compared to corresponding non-tumor tissue. High NEAT1 expression was associated with tumor progression and poor survival in RCC patients. NEAT1 knockdown suppressed RCC cell proliferation by inhibiting cell cycle progression, and inhibited RCC cell migration and invasion by reversing the epithelial-to- mesenchymal transition phenotype...
May 10, 2017: Oncotarget
https://www.readbyqxmd.com/read/28544627/epithelial-to-mesenchymal-transition-transcription-factors-in-cancer-associated-fibroblasts
#3
REVIEW
Josep Baulida
Beyond inducing epithelial-to-mesenchymal transcription (EMT), transcriptional factors of the Snail, ZEB and Twist families (EMT-TFs) control global plasticity programs affecting cell stemness and fate. Literature addressing the reactivation of these factors in adult tumour cells is very extensive, as they enable cancer cell plasticity and fuel both tumour initiation and metastatic spread. Incipient data reveal that EMT-TFs are also expressed in fibroblasts, providing these with additional properties. Here, I will review recent reports on the expression of EMT-TFs in cancer-associated fibroblasts (CAFs)...
May 24, 2017: Molecular Oncology
https://www.readbyqxmd.com/read/28544576/atypical-adenoma-of-the-thyroid-diagnosed-as-anaplastic-cancer-by-cytopathology
#4
Hiromitsu Hatakeyama, Kimiko Hoshino, Kenji Mizoguchi, Takayoshi Suzuki, Kanako C Hatanaka, Yukie Yamaya, Satoshi Kano, Takatsugu Mizumachi, Akihiro Homma
Atypical adenoma of the thyroid is a rare form of tumor, and its accurate diagnosis prior to surgical resection is difficult as the histological and pathological morphologies are very similar to those of anaplastic thyroid carcinoma (ATC), and its anaplastic transformation remains to be elucidated. We reported a case of a 75-year-old female with a thyroid isthmus nodule diagnosed repeatedly by FNAC as anaplastic carcinoma. Both the first and second FNAC specimen slides showed a large number of scattered or aggregated atypical cells consisting of large, pleomorphic nuclei with irregular membranes, chromatin clumps and prominent nucleoli...
May 22, 2017: Diagnostic Cytopathology
https://www.readbyqxmd.com/read/28544498/cancer-metastasis-through-the-prism-of-epithelial-to-mesenchymal-transition-in-circulating-tumor-cells
#5
REVIEW
Douglas S Micalizzi, Daniel A Haber, Shyamala Maheswaran
Metastasis of epithelial cancer cells to distal sites is a particularly critical stage of cancer progression that typically marks the incurability of the disease. It is governed by a complex series of events including invasion and intravasation of tumor cells into the stroma and blood, respectively. Epithelial to mesenchymal transition (EMT), a phenotypic change marked by the loss of epithelial characteristics and the acquisition of invasive mesenchymal properties, is implicated in the dissemination of tumor cells...
May 24, 2017: Molecular Oncology
https://www.readbyqxmd.com/read/28544415/inertial-microfluidic-cell-stretcher-imcs-fully-automated-high-throughput-and-near-real-time-cell-mechanotyping
#6
Yanxiang Deng, Steven P Davis, Fan Yang, Kevin S Paulsen, Maneesh Kumar, Rebecca Sinnott DeVaux, Xianhui Wang, Douglas S Conklin, Assad Oberai, Jason I Herschkowitz, Aram J Chung
Mechanical biomarkers associated with cytoskeletal structures have been reported as powerful label-free cell state identifiers. In order to measure cell mechanical properties, traditional biophysical (e.g., atomic force microscopy, micropipette aspiration, optical stretchers) and microfluidic approaches were mainly employed; however, they critically suffer from low-throughput, low-sensitivity, and/or time-consuming and labor-intensive processes, not allowing techniques to be practically used for cell biology research applications...
May 23, 2017: Small
https://www.readbyqxmd.com/read/28544403/serine-protease-inhibitor-kazal-type-1-spink1-downregulates-e-cadherin-and-induces-emt-of-hepatoma-cells-to-promote-hepatocellular-carcinoma-metastasis-via-the-mek-erk-signaling-pathway
#7
Ying Hai-Yan, Gong Chao-Jie, Feng Yi, Jing Da-Dao, Lun-Gen Lu
OBJECTIVE: To investigate serine protease inhibitor Kazal type 1(SPINK1) expression and its influence on the prognosis of human hepatocellular carcinoma(HCC) and to explore the underlying molecular mechanisms involved. METHODS: Eighty patients with HCC who underwent curative resection were followed-up with a median observation time of 58.6 months.SPINK1expression was detected in the primary HCC samples by immunohistochemistry . Its role in tumor invasion and metastasis was evaluated in vitro by gene silence using siRNA-mediated approach, rSPINK1 and U0126(an inhibitor of MEK/ERK)...
May 23, 2017: Journal of Digestive Diseases
https://www.readbyqxmd.com/read/28543447/fas-signaling-induces-stemness-properties-in-colorectal-cancer-by-regulation-of-bmi1
#8
Jiaxuan Chen, Yadong Wang, Linghao Zhuo, Zhizhong Liu, Tao Liu, Wenjing Li, Yidong Cai, Haoxuan Zheng
Fas signaling promotes colorectal cancer (CRC) metastasis by inducing epithelial- mesenchymal transition (EMT). The acquisition of EMT properties in turn induces stemness but the mechanism by which Fas signaling contributes to it still remains unclear. Hence, the aim of this study was to investigate how Fas signaling regulates CRC stemness. For this purpose, soft agar assay, sphere formation assay, cell survival analysis, immunoblot, qRT-PCR, chromatin immunoprecipitation, and luciferase reporter assay were performed...
May 25, 2017: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/28542651/cellular-migration-transition-and-interaction-during-regeneration-of-the-sponge-hymeniacidon-heliophila
#9
Cristiano C Coutinho, Ivone de Andrade Rosa, John Douglas de Oliveira Teixeira, Leonardo R Andrade, Manoel Luis Costa, Claudia Mermelstein
Sponges have a high capacity for regeneration and this process improves biomass production in some species, thus contributing to a solution for the biomass supply problem for biotechnological applications. The aim of this work is to characterize the dynamics of cell behavior during the initial stages of sponge regeneration, using bright-field microscopy, confocal microscopy and SEM. We focused on the first 20 h of regeneration, during which blastema formation and epithelium initialization occur. An innovative sponge organotypic culture of the regenerating internal region is described and investigated by confocal microscopy, cell transplantation and vital staining...
2017: PloS One
https://www.readbyqxmd.com/read/28542577/leptin-signals-via-tgfb1-to-promote-metastatic-potential-and-stemness-in-breast-cancer
#10
Ameet K Mishra, Christopher R Parish, Ma-Li Wong, Julio Licinio, Anneke C Blackburn
Epidemiological studies have shown obesity to be linked with poorer outcomes in breast cancer patients. The molecular mechanisms responsible for the increased risk of invasive/metastatic disease with obesity are complex, but may include elevated levels of adipokines such as leptin. Using physiological levels of leptin found in obesity in a novel chronic in vitro treatment model (≤200 ng/ml for 14 days), we confirmed the occurrence of leptin-mediated changes in growth, apoptosis and metastatic behavior, and gene expression changes representing epithelial-to-mesenchymal transition (EMT) and a cancer stem cell (CSC) like phenotype in breast epithelial and cancer cell lines (MCF10A, MCF10AT1, MCF7 and MDA-MB-231)...
2017: PloS One
https://www.readbyqxmd.com/read/28542487/characterization-of-micrornas-expression-profiles-in-human-dental-derived-pluripotent-stem-cells
#11
Xiaobing Tan, Qingyuan Dai
Induced pluripotent stem cells (iPSCs) technology provides a powerful means to generate and regenerate unlimited pluripotent stem cells directly from body tissue cells. Stem cells from apical papilla (SCAP) and Dental pulp stem cells (DPSCs) are present in 'cell-rich zones' within the dental pulp region, which are capable of regenerating pulp and dentin tissues in vivo. In this study, we investigated the difference of miRNAs expression in SCAPs and DPSCs before and after the reprogramming. Using miRNA microarray, 134 and 265 differentially expressed miRNAs in DPSCs- and SCAP-iPSCs were up-regulated compared to these before reprogramming...
2017: PloS One
https://www.readbyqxmd.com/read/28542402/evidence-for-pleural-epithelial-mesenchymal-transition-in-murine-compensatory-lung-growth
#12
Alexandra B Ysasi, Willi L Wagner, Cristian D Valenzuela, Arne Kienzle, Andrew B Servais, Robert D Bennett, Akira Tsuda, Maximilian Ackermann, Steven J Mentzer
In many mammals, including rodents and humans, removal of one lung results in the compensatory growth of the remaining lung; however, the mechanism of compensatory lung growth is unknown. Here, we investigated the changes in morphology and phenotype of pleural cells after pneumonectomy. Between days 1 and 3 after pneumonectomy, cells expressing α-smooth muscle actin (SMA), a cytoplasmic marker of myofibroblasts, were significantly increased in the pleura compared to surgical controls (p < .01). Scanning electron microscopy of the pleural surface 3 days post-pneumonectomy demonstrated regions of the pleura with morphologic features consistent with epithelial-mesenchymal transition (EMT); namely, cells with disrupted intercellular junctions and an acquired mesenchymal (rounded and fusiform) morphotype...
2017: PloS One
https://www.readbyqxmd.com/read/28542126/human-mscs-promotes-colorectal-cancer-epithelial-mesenchymal-transition-and-progression-via-ccl5-%C3%AE-catenin-slug-pathway
#13
Ke Chen, Qianqian Liu, Lai Ling Tsang, Qiao Ye, Hsiao Chang Chan, Yunwei Sun, Xiaohua Jiang
Mesenchymal stem cells (MSCs) extensively interact with cancer cells and other stroma cells in the tumor microenvironment. However, the role of MSCs in colorectal cancer (CRC) progression and metastasis is controversial. This study was designed to identify the role of inflammation-activated-MSCs in CRC development. Our results show that tumor necrosis factor (TNF)-α-preactivated-hMSCs significantly promote the progression of colon cancer cells by enhancing cell proliferation, epithelial-mesenchymal transition, migration, and invasion...
May 25, 2017: Cell Death & Disease
https://www.readbyqxmd.com/read/28540808/exposure-to-febrile-range-hyperthermia-potentiates-wnt-signalling-and-epithelial-mesenchymal-transition-gene-expression-in-lung-epithelium
#14
Ratnakar Potla, Mohan E Tulapurkar, Irina G Luzina, Sergei P Atamas, Ishwar S Singh, Jeffrey D Hasday
BACKGROUND: As environmental and body temperatures vary, lung epithelial cells experience temperatures significantly different from normal core temperature. Our previous studies in human lung epithelium showed that: (i) heat shock accelerates wound healing and activates profibrotic gene expression through heat shock factor-1 (HSF1); (ii) HSF1 is activated at febrile temperatures (38-41 °C) and (iii) hypothermia (32 °C) activates and hyperthermia (39.5 °C) reduces expression of a subset of miRNAs that target protein kinase-Cα (PKCα) and enhance proliferation...
April 26, 2017: International Journal of Hyperthermia
https://www.readbyqxmd.com/read/28540637/importance-of-human-peritoneal-mesothelial-cells-in-the-progression-fibrosis-and-control-of-gastric-cancer-inhibition-of-growth-and-fibrosis-by-tranilast
#15
Hiroto Saito, Sachio Fushida, Shinichi Harada, Tomoharu Miyashita, Katsunobu Oyama, Takahisa Yamaguchi, Tomoya Tsukada, Jun Kinoshita, Hidehiro Tajima, Itasu Ninomiya, Tetsuo Ohta
BACKGROUND: Scirrhous gastric cancer is an intractable disease with a high incidence of peritoneal dissemination and obstructive symptoms (e.g., ileus, jaundice, and hydronephrosis) arising from accompanying marked fibrosis. Microenvironmental interactions between cancer cells and cancer-associated fibroblasts are the suggested cause of the disease. We elucidated the mechanisms of tumor growth and fibrosis using human peritoneal mesothelial cells (HPMCs) and investigated the effects of tranilast treatment on cells and a xenograft mouse model of fibrosis...
May 24, 2017: Gastric Cancer
https://www.readbyqxmd.com/read/28539961/yiqi-huayu-jiedu-decoction-inhibits-the-invasion-and-metastasis-of-gastric-cancer-cells-through-tgf-%C3%AE-smad-pathway
#16
Ting-Ting Wu, Jun Lu, Pei-Qiu Zheng, Shen-Lin Liu, Jian Wu, Wei Sun, Qing-Min Sun, Nai-Xia Ma, Xue-Lian Ding, Min Chen, Xi Zou
Background. Yiqi Huayu Jiedu Decoction (YHJD) can obviously improve the quality of life of those patients with gastric cancer and prolong their survival. Methods. In vitro experiments, we observe YHJD's effect on the cells' proliferation by MTT assay. Cell adhesion assay, wound-healing assay, and Transwell invasion assay serve to detect its influence on cells' adhesion, migration, and invasion, respectively. Inhibitor (10 μM/L of SB431542) and activator (10 ng/mL of TGF-β) of TGF-β/Smad pathway were used to estimate whether YHJD's impact on the biological behavior of gastric cancer cells was related to TGF-β/Smad pathway...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28539833/sdf-1-in-mammary-fibroblasts-of-bovine-with-mastitis-induces-emt-and-inflammatory-response-of-epithelial-cells
#17
Guiliang He, Mengru Ma, Wei Yang, Hao Wang, Yong Zhang, Ming-Qing Gao
Fibroblasts constitute the majority of the stromal cells within bovine mammary gland, yet the functional contributions of these cells to mastitis and fibrosis and the mechanism are poorly understood. In this study, we demonstrate that inflammation-associated fibroblasts (INFs) extracted from bovine mammary glands with clinical mastitis had different expression pattern regarding to several extracellular matrix (ECM) proteins, chemokines and cytokines compared to normal fibroblasts (NFs) from dairy cows during lactation...
2017: International Journal of Biological Sciences
https://www.readbyqxmd.com/read/28539335/special-k-once-the-fun-is-over-an-emt-arrives-for-the-bladder
#18
Warren G Hill, Weiqun Yu
Wang et al. report that in rats exposed to daily ketamine for sixteen weeks, the urothelium begins to undergo phenoconversion to a more mesenchymal state. This epithelial to mesenchymal transition (EMT), has several classic molecular hallmarks and Wang et al. demonstrate convincingly that a number of these including downregulation of E-cadherin, upregulation of mesenchymal markers vimentin and fibroblast specific protein (FSP-1), colocalization of both epithelial and mesenchymal markers in a subset of urothelium-associated cells and upregulation of TGFβ expression in urothelium are all present...
May 24, 2017: American Journal of Physiology. Renal Physiology
https://www.readbyqxmd.com/read/28539268/mir-221-mediates-the-epithelial-mesenchymal-transition-of-hepatocellular-carcinoma-by-targeting-adipor1
#19
Tong Li, Min Li, Shaobo Hu, Xiang Cheng, Yang Gao, Shuai Jiang, Qihong Yu, Chen Zhang, Ping Sun, Wenjing Xian, Zifang Song, Yong Zhang, Qichang Zheng
Recent studies have shown that miRNAs play vital roles in tumorigenesis. However, their effects on the epithelial-mesenchymal transition (EMT) in hepatocellular carcinoma (HCC) need to be better understood. Our present study demonstrates that miR-221, which is overexpressed in HCC tissues, promotes EMT in HCC cell lines by targeting a new gene, AdipoR1. First, overexpression of miR-221 was identified in 40 pairs of human HCC tumor and matched normal tissues. Moreover, we found that elevated miR-221 was strongly associated with worse clinicopathologic features in HCC patients...
May 21, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28537678/lncrna-bcar4-promotes-proliferation-invasion-and-metastasis-of-non-small-cell-lung-cancer-cells-by-affecting-epithelial-mesenchymal-transition
#20
N Li, W-J Gao, N-S Liu
OBJECTIVE: Long non-coding RNAs (lncRNAs) play an important role in various cellular biological processes. It is also involved in the occurrence and development of the tumor. BCAR4 is reported to be highly expressed in breast cancer and promotes cell proliferation. However, the biological effects of BCAR4 in non-small cell lung cancer (NSCLC) remains unclear. PATIENTS AND METHODS: qRT-PCR was performed for detecting BCAR4 expression in 76 pairs of NSCLC tissues and corresponding cancer-adjacent tissues and 6 NSCLC cell lines...
May 2017: European Review for Medical and Pharmacological Sciences
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