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Liwen Zhao, Jing Quan, Zuwei Li, Xiang Pan, Jingyao Wang, Jinling Xu, Weijie Xu, Xin Guan, Hang Li, Shangqi Yang, Yaoting Gui, Yun Chen, Yongqing Lai
The aim of the present study was to investigate the role of microRNA (miR)‑222‑3p in renal cell carcinoma (RCC). The expression level of miR‑222‑3p was detected in RCC tissues and cell lines (ACHN, 786‑O, Caki‑1 and 769‑P) and was identified to be significantly upregulated compared with the level in adjacent normal renal tissues and HK‑2 cells. Further in vitro experiments demonstrated that the over-expression of miR‑222‑3p promoted the migration and invasion, and attenuated the apoptosis of 786‑O cells, whereas the knockdown of miR‑222‑3p suppressed the migration and invasion and induced the apoptosis of 786‑O cells...
October 15, 2018: International Journal of Molecular Medicine
Lei Liu, Qifei Wang, Jun Mao, Tao Qin, Yan Sun, Jinfeng Yang, Yanyan Han, Lianhong Li, Quanlin Li
Metastatic Renal cell carcinoma (RCC) remains a difficult oncologic challenge. Salinomycin is a monocarboxylic polyether antibiotic, which has been proved to possess anti-tumor activities in multiple types of cancer cells. However, its effects on RCC cells remains unclear. In our study, salinomycin could inhibit the proliferation and viability of RCC cell lines 786-O and ACHN. The TUNEL assay revealed that treatment with salinomycin induced DNA breaking in RCC cells. Consistently, Western blotting showed up-regulation of pro-apoptotic biomarkers (cleaved caspase3/9 and cleaved PARP1) and down-regulation of anti-apoptotic biomarker (survivin) in RCC cells after salinomycin treatment, suggesting that salinomycin could induce RCC cell apoptosis...
September 28, 2018: Chemico-biological Interactions
Yujiao Zhang, Yizeng Fan, Shangke Huang, Guanying Wang, Rui Han, Fuxi Lei, Anqi Luo, Xin Jing, Lin Zhao, Shanzhi Gu, Xinhan Zhao
Thymoquinone (TQ, 2-methyl-5-isopropyl-1,4-benzoquinone), a bioactive constituent extracted from the seeds of Nigella sativa, has been proved to exert anti-tumor efficiency in various cancers. Autophagy is a self-digestion phenomenon and its role in tumor formation and progression remains to be controversial. In the present study, we investigated the effects of TQ on RCC cell lines (786-O and ACHN) using wound healing assay, Transwell assay and western blot. Then we found TQ effectively inhibited metastatic capacities of RCC cells in vitro, which was also verified in xenograft model...
September 27, 2018: Cancer Science
Wenting Deng, Wei Han, Tao Fan, Xiaoku Wang, Zhao Cheng, Bo Wan, Jinlian Chen
BACKGROUND: Scutellarin is a naturally flavone glycoside that has been shown to exhibit anti-proliferative and anti-apoptotic activities among various human malignancies. However, the anti-cancer effect of Scutellarin in Renal cell carcinoma (RCC) and the underlying mechanism remains unclear. METHODS AND MATERIALS: RCC cell lines ACHN and 786-O were treated with different concentrations (0-210 μM) of Scutellarin in vitro. Cell viability and proliferation were investigated by MTT and colony formation assays...
November 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Sateesh Kumar Arepalli, Chaerim Lee, Seongrak Sim, Kiho Lee, Hyunji Jo, Kyu-Yeon Jun, Youngjoo Kwon, Jong-Soon Kang, Jae-Kyung Jung, Heesoon Lee
A novel series of 35 angularly fused pentacyclic 13H-benzo[f]chromeno[4,3-b][1,7]naphthyridines and 13H-benzo[f]chromeno[4,3-b][1,7]naphthyridin-5-ium chlorides were designed and synthesized. Their cytotoxic activities were investigated against six human cancer cell lines (NCIH23, HCT15, NUGC-3, ACHN, PC-3, and MDA-MB-231). Among all screened compounds; 28, 30, 34, 35, 46, 48, 52, and 53 compounds exhibited potent cytotoxic activities against all tested human cancer cell lines. Further, these potent lead cytotoxic agents were evaluated against human Topoisomerase I and IIα inhibition...
September 18, 2018: Bioorganic & Medicinal Chemistry
Hua-Wen Cheng, Jian-Guang He, Wen-Yan He
The main objective of the present research work was to evaluate the antitumor effects of ethanol extract of Semen Euphorbiae (EESE) in ACHN human renal carcinoma cells. The effects on apoptosis induction, cell cycle phase distribution and livin protein expression were also evaluated. MTT assay was used to assess cytotoxic effects of the extract while as clonogenic assay was used to evaluate effects on colony formation tendency. Inverted phase contrast and fluorescence microscopic techniques were used to evaluate effects of EESE on cellular morphology and apoptosis...
September 2018: Saudi Journal of Biological Sciences
Michihiro Yabe, Kei Ishibashi, Akifumi Onagi, Ryo Tanji, Ruriko Honda-Takinami, Tomoyuki Koguchi, Kanako Matsuoka, Seiji Hoshi, Junya Hata, Masao Kataoka, Soichiro Ogawa, Hiroyuki Hiraki, Nobuhiro Haga, Yoshiyuki Kojima
Background: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is a tumor-selective apoptosis inducer that is expressed in natural killer cells, whose cytotoxicity is activated by interferon (IFN). We investigated the effect of suppressor of cytokine signaling (SOCS) 3 on the expression of TRAIL receptors (DR4) and on TRAIL sensitivity in renal cell carcinoma (RCC) cells. Methods: Vector expression, RNA interference and IL-6 receptor antibody tocilizumab were used to investigate the functional role of SOCS3 in DR4 expression...
August 3, 2018: Oncotarget
Canbin Lin, Zuwei Li, Peijie Chen, Jing Quan, Xiang Pan, Liwen Zhao, Liang Zhou, Yulin Lai, Tao He, Weijie Xu, Jinling Xu, Xin Guan, Hang Li, Shangqi Yang, Yimin Hu, Yongqing Lai
AIMS: In adult population, the renal cell carcinoma (RCC) is one of the most common urological malignancies. It is meaningful to research for the molecular markers which are involved in the occurrence and development of RCC. Therefore, we concentrate on illuminating the role of microRNA-154-5p in progression of RCC and explore its prognostic values. MAIN METHODS: The real-time quantitative polymerase chain reaction (RT-qPCR) was applied to determine expression level of miR-154-5p in tissues...
September 15, 2018: Life Sciences
Xiang Pan, Zuwei Li, Liwen Zhao, Jing Quan, Liang Zhou, Jinling Xu, Weiji Xu, Xin Guan, Hang Li, Shangqi Yang, Yaoting Gui, Yongqing Lai
Renal cell carcinoma (RCC) is the third most common urological malignancy in the USA and represents 2‑3% of all adult malignancies. Furthermore, the incidence of RCC has been progressively increasing over recent years. Although the morbidity of treatment has decreased with the use of multidisciplinary synthetic therapy, the prognosis of terminal cancer remains poor, with a 5‑year survival rate of 5‑10%. MicroRNAs (miRs) have been correlated with the regulation of 30‑60% of the protein-coding genes and act as oncogenes or anti‑oncogenes in RCC...
August 17, 2018: Oncology Reports
Jinlin Xie, Wenfeng Lin, Linglong Huang, Naijin Xu, Abai Xu, Binshen Chen, Masami Watanabe, Chunxiao Liu, Peng Huang
Bufalin, one of the active ingredients of the Chinese drug Chan su, exhibits significant antitumor activity against various cancer types. However, the role of bufalin in renal cell carcinoma (RCC) remains unclear. In the present study, it was demonstrated that bufalin inhibited cell proliferation, blocked the cell cycle in the G2/M phase, and reduced the metastasis of human RCC ACHN cells via the upregulation of p21waf/cip1 and E-cadherin and the downregulation of cyclin dependent kinase 1, cyclin B1, N-cadherin, and hypoxia-inducible factor-1α (HIF-1α)...
September 2018: Oncology Letters
Karthikeyan Rajamani, Somasundaram S Thirugnanasambandan
In our previous work, we assessed the ameliorative effect of crude extract from Padina boergesenii. In this report, we have extended these study by fractionating the methanol extract with methanol:ethylacetate (1:3,v/v) (MME-F) and assessed the cytotoxic effect of MME-F fraction in human renal carcinoma cell lines (A498 and ACHN). The fraction had time-and dose-dependent inhibition of cancer cell proliferation, migration with deceleration of cancer growth at EC50 -22.73 μg in A498 and 26.43 μg in ACHN cells...
November 2018: Environmental Toxicology
Yue Pan, Yongjin Wu, Jialei Hu, Yujia Shan, Jia Ma, Huipeng Ma, Xia Qi, Li Jia
OBJECTIVES: Accumulating evidence demonstrated that the long noncoding RNA (lncRNA) HOTAIR (Hox transcript antisense intergenic RNA) plays key role in renal cell carcinoma (RCC) malignancy, while microRNA-124 (miR-124) is a tumour suppressor in RCC. The aim of this work was to assess the biological function of HOTAIR and to explore underlying mechanism involved in HOTAIR/miR-124/alpha-2, 8-sialyltransferase 4 (ST8SIA4) axis-regulated progression in RCC. MATERIALS AND METHODS: Real-time PCR analyses and western blots were performed to the levels of HOTAIR, miR-124 and ST8SIA4 expression in human RCC tissues and RCC cell lines (ACHN and 786-O)...
August 13, 2018: Cell Proliferation
Dejiao He, Xuan Sun, Hongxia Yang, Xiaoli Li, Dingping Yang
Cancer cells usually have a high requirement for fatty acids in order to meet the rapid proliferation and metabolism. Acetyl-CoA carboxylase-α(ACCA) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA and has been a rate-limiting enzyme in the synthesis of long chain fatty acid and cellular energy storage. 5-tetradecyloxy-2-furoic acid (TOFA) is well known as an allosteric inhibitor of ACCA. In this study, we examined the functions of TOFA in human renal cell carcinoma (RCC) cell lines ACHN and 786-O...
2018: Journal of Cancer
Shiyue Zhao, Yangwei Wang, Yan Lou, Yonggang Wang, Jing Sun, Manyu Luo, Wen Li, Lining Miao
An increasing body of evidence has indicated that microRNAs (miRNAs/miRs) may play an important role in tumourigenesis and tumour progression. Recent studies have demonstrated that miR‑320a is aberrantly expressed in a variety of different types of human cancer. The results of the present study confirmed that the expression of miR‑320a was decreased in clinical specimens and cell lines. Expression of miR‑320a inhibited the growth and invasive ability of ACHN and Caki‑1 cells. Bioinformatics analysis and a luciferase reporter assay demonstrated that forkhead box protein M1 (FoxM1) was directly regulated by miR‑320a...
October 2018: Oncology Reports
Leticia Serrano-Oviedo, Marta Ortega-Muelas, Jesús García-Cano, María Ll Valero, Francisco J Cimas, Raquel Pascual-Serra, Diego M Fernandez-Aroca, Olga Roche, María J Ruiz-Hidalgo, Borja Belandia, José M Giménez-Bachs, Antonio S Salinas, Ricardo Sanchez-Prieto
OBJECTIVES: To fully clarify the role of Mitogen Activated Protein Kinase in the therapeutic response to Sorafenib in Renal Cell Carcinoma as well as the cell death mechanism associated to this kinase inhibitor, we have evaluated the implication of several Mitogen Activated Protein Kinases in Renal Cell Carcinoma-derived cell lines. MATERIALS AND METHODS: An experimental model of Renal Cell Carcinoma-derived cell lines (ACHN and 786-O cells) was evaluated in terms of viability by MTT assay, induction of apoptosis by caspase 3/7 activity, autophagy induction by LC3 lipidation, and p62 degradation and kinase activity using phospho-targeted antibodies...
2018: PloS One
K Olofsson, V Carannante, M Ohlin, T Frisk, K Kushiro, M Takai, A Lundqvist, B Önfelt, M Wiklund
Understanding the complex 3D tumor microenvironment is important in cancer research. This microenvironment can be modelled in vitro by culturing multicellular tumor spheroids (MCTS). Key challenges when using MCTS in applications such as high-throughput drug screening are overcoming imaging and analytical issues encountered during functional and structural investigations. To address these challenges, we use an ultrasonic standing wave (USW) based MCTS culture platform for parallel formation, staining and imaging of 100 whole MCTS...
August 7, 2018: Lab on a Chip
Liang Zhou, Xiang Pan, Zuwei Li, Peijie Chen, Jing Quan, Canbin Lin, Yulin Lai, Jinling Xu, Weijie Xu, Xin Guan, Hang Li, Yaoting Gui, Yongqin Lai
BACKGROUND: MicroRNA(miRNA) plays a key regulatory role in various stages of tumorigenesis, including cell growth, cell cycle control, apoptosis avoidance, tissue invasion, and metastasis. Several microRNAs are involved in the development of renal cell carcinoma (RCC) and the malignant transformation process. However, the effects of miR-663a on RCC have rarely been reported. METHODS: In the present study, the expression of miR-663a was examined in RCC using matched normal kidney tissues and four cell lines (786-O, Caki-1, ACHN and HK-2)...
September 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Jing Quan, Yawen Li, Xiang Pan, Yulin Lai, Tao He, Canbin Lin, Liang Zhou, Liwen Zhao, Shuolei Sun, Yu Ding, Lingzhi Tao, Yimin Hu, Xionghui Wu, Zebo Chen, Fangting Zhang, Jing Ye, Liangchao Ni, Yongqing Lai
An increasing number of studies have demonstrated the function of microRNAs (miRNAs) in the initiation and development of various types of cancer. Among them, miR-425-5p is proven to serve an important function in several types of cancer, including gastric, cervical cancer, and hepatocellular carcinoma. However, the function of miR-425-5p in renal cell carcinoma (RCC) remains unclear. In the present study, it was demonstrated that the expression level of miR-425-5p was upregulated in RCC tissues and cell lines compared with normal tissues and cell lines (P<0...
August 2018: Oncology Letters
Seok Kim, Seung Un Seo, Kyoung-Jin Min, Seon Min Woo, Ju-Ock Nam, Peter Kubatka, Shin Kim, Jong-Wook Park, Taeg Kyu Kwon
Garcinol is a polyisoprenylated benzophenone derived from the Garcinia indica fruit that possess potential therapeutic effects such as inhibition of inflammation and tumor expansion. Here, we investigated whether garcinol induces TRAIL sensitization in renal carcinoma cells. Single treatment with garcinol or TRAIL did not effect on apoptosis. However, combined treatment with garcinol plus TRAIL significantly induced apoptosis in renal carcinoma (Caki, ACHN and A498), lung carcinoma (A549), and hepatoma (SK-Hep1) cells...
July 2, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Yun-Sok Ha, Yeon-Yong Kim, Na Hee Yu, So Young Chun, Seock Hwan Choi, Jun Nyung Lee, Bum Soo Kim, Eun Sang Yoo, Tae Gyun Kwon
Purpose: Transient receptor potential melastatin member 7 (TRPM7), an ion channel and serine/threonine protein kinase, has been linked with distinct human malignancies. However, the role of TRPM7 in renal cell carcinoma (RCC) has not been investigated. The aim of this study is to determine whether TRPM7 regulates the migration and invasion of RCC cells. Its relationship with signal transduction pathways was also studied. Materials and Methods: The human RCC cell lines ACHN and SN12C were chosen for this study...
July 2018: Investigative and Clinical Urology
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