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cerebral ischemia-reperfusion

Lijun Hu, Yun Wang, Yihui Zhang, Ning Yang, Huijie Han, Yuanyuan Shen, Derong Cui, Shengrong Guo
2-Methoxyestradiol (2ME2), as a microtubule and hypoxia-inducible factor-1 (HIF-1) inhibitor, can be used to treat cerebral ischemia-reperfusion (I/R) injury. However, its poor water solubility compromises its efficacy as a neuroprotectant. Herein, we synthesized PEGylated 2ME2 and angiopep-2 capped PEGylated 2ME2 and fabricated angiopep-2 modified PEGylated 2ME2 micelles containing free 2ME2 (ANG-PEG-2ME2/2ME2) via emulsion-solvent evaporation method. The effect of the micelles on ischemia-reoxygenation injury was evaluated by oxygen-glucose deprivation/reoxygenation (OGD/R) models with different degrees of PC12 cell damage...
August 8, 2018: Colloids and Surfaces. B, Biointerfaces
Wenzhu Wang, Lixu Liu, Chen Chen, Peng Jiang, Tong Zhang
Global cerebral ischemia-reperfusion (GCI/R) may occur after any of several clinical conditions such as cardiac arrest and anesthetic accident. Some dopamine receptor agonists possess neuroprotective effects. However, some of them may produce side effects during treatment.Piribedil, which is a dopamine D2/D3 receptor agonist, has fewer side effects and is well tolerated. This study investigated the effects of piribedil on learning and memory of rats with GCI/R according to modified neurological severity score (mNSS) scoring and Morris water maze test (MWM)...
August 11, 2018: Neuroscience Letters
Zhenhong Wang, Wei Zhou, Haiping Dong, Xiaoxiao Ma, Zhenzhou He
Focal ischemia/reperfusion (I/R) injury induced cerebral inflammation, aggravates brain damage. The aim of the present study was to investigate the protective mechanisms of dexmedetomidine (DEX) on I/R brain injury in rats. Sprague‑Dawley rats were divided to seven experimental groups (18 rats/group): Sham surgery; middle cerebral artery occlusion (MCAO) surgery (90 min); DEX10 [10 µg/kg intraperitoneal (i.p.) injection 30 min prior to MCAO]; DEX50 (50 µg/kg i.p. 30 min prior to MCAO); DEX100 (100 µg/kg i...
August 3, 2018: Molecular Medicine Reports
Jing Han, Ji-Zhou Zhang, Zhi-Feng Zhong, Zuan-Fang Li, Wen-Sheng Pang, Juan Hu, Li-Dian Chen
Recovery following stroke involves neurogenesis and axonal remodeling within the ischemic brain. Gualou Guizhi decoction (GLGZD) is a Chinese traditional medicine used for the treatment of post-stroke limb spasm. GLGZD has been reported to have neuroprotective effects in cerebral ischemic injury. However, the effects of GLGZD on neurogenesis and axonal remodeling following cerebral ischemia remain unknown. In this study, a rat model of focal cerebral ischemia/reperfusion was established by middle cerebral artery occlusion...
August 2018: Neural Regeneration Research
Jing-Bo Sun, Yan Li, Ye-Feng Cai, Yan Huang, Shu Liu, Patrick Kk Yeung, Min-Zhen Deng, Guang-Shun Sun, Prince Lm Zilundu, Qian-Sheng Hu, Rui-Xin An, Li-Hua Zhou, Li-Xin Wang, Xiao Cheng
Scutellarin, a bioactive flavone isolated from Scutellaria baicalensis, has anti-inflammatory, anti-neurotoxic, anti-apoptotic and anti-oxidative effects and has been used to treat cardiovascular and cerebrovascular diseases in China. However, the mechanisms by which scutellarin mediates neuroprotection in cerebral ischemia remain unclear. The interaction between scutellarin and nicotinamide adenine dinucleotide phosphate oxidase 2 (NOX2) was assessed by molecular docking study, which showed that scutellarin selectively binds to NOX2 with high affinity...
August 2018: Neural Regeneration Research
Yu Liu, Yaoxian Zhang, Zihao Zheng, Xicheng Liu
The aim of this research investigation was to profound analysis the mitigating impact of sevoflurane/arginine post-molding on cerebral ischemia-reperfusion damage in rats. The authors fabricated emulsions fusing sevoflurane, perfluorooctyl bromide as a settling specialist, and mixes of arginine polymer. Cell suitability and gene expression of tubulin and NeuN were assessed. The stability, morphology and functional group were evaluated utilizing dynamic light scattering (DLS), Transmission Electron Microscope (TEM), atomic force microscopy (AFM), and Fourier-transform infrared spectroscopy (FTIR)...
July 18, 2018: Journal of Photochemistry and Photobiology. B, Biology
Zhewei Zhao, Chengran Lu, Tianjia Li, Wenda Wang, Wei Ye, Rong Zeng, Leng Ni, Zhichao Lai, Xuebin Wang, Changwei Liu
Carotid endarterectomy (CEA) is the treatment of choice for carotid stenosis. Some patients develop ischemia and reperfusion (I/R) injury after CEA. This study was designed to investigate the neuroprotective effects of melatonin on I/R injury in both rats and humans. To this end, 36 male rats were evaluated, and a double-blind randomized controlled trial (RCT) including 60 patients was performed. A rat model of middle cerebral artery occlusion (MCAO) was used to mimic cerebral I/R. After 2 hr of occlusion and 24 hr of reperfusion, blood samples and brain tissues were harvested for further assessments...
August 11, 2018: Journal of Pineal Research
Mahsa Sarami Foroshani, Zeinab Sadat Sobhani, Mohammad Taghi Mohammadi, Masiha Aryafar
BACKGROUND: The present study aimed to examine the protective role of fullerenol nanoparticles against blood-brain barrier (BBB) interruption and brain edema during cerebral ischemia-reperfusion injury probably by reduction of interleukin-6 (IL-6) and matrix metalloproteinase-9 (MMP-9) transcription. METHODS: The male Wistar rats (weighting 280-320 g) were randomly assigned into four groups as follows: sham, control ischemic, pretreated ischemic, and posttreated ischemic groups...
August 6, 2018: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
Jordi Pedragosa, Angélica Salas-Perdomo, Mattia Gallizioli, Roger Cugota, Francesc Miró-Mur, Ferran Briansó, Carles Justicia, Fernando Pérez-Asensio, Leonardo Marquez-Kisinousky, Xabier Urra, Anna Gieryng, Bozena Kaminska, Angel Chamorro, Anna M Planas
The central nervous system (CNS) contains several types of immune cells located in specific anatomic compartments. Macrophages reside at the CNS borders surrounding the brain vessels, in leptomeningeal spaces and the choroid plexus, where they interact with the vasculature and play immunological surveillance and scavenging functions. We investigated the phenotypic changes and role of these macrophages in response to acute ischemic stroke. Given that CD163 expression is a hallmark of perivascular and meningeal macrophages in the rat and human brain, we isolated CD163+ brain macrophages by fluorescence activated cell sorting...
August 9, 2018: Acta Neuropathologica Communications
Thomas Raphael Meinel, Frauke Pult, Jan Gralla, Marcel Arnold, Claudio Bassetti, Simon Jung
A 76-year-old man with a history of arterial hypertension, obstructive sleep apnea, dyslipidemia, family history of cardiovascular events, prestroke and overweight presented 90 minutes after acute onset of right-sided sensorimotor hemiparesis, hemiataxia and dysarthria (National Institutes of Health Stroke Scale (NIHSS) 9/42). Magnetic resonance imaging (MRI) revealed a pontine ischemia and MR angiography showed a thrombus in the middle to distal portion of the basilar artery. Owing to the location, an occlusion of one lumen of a fenestrated basilar artery was suspected...
August 9, 2018: Interventional Neuroradiology
Pian Gong, Zhan Zhang, Changlin Zou, Qi Tian, Xuemei Chen, Michael Hong, Xi Liu, Qianxue Chen, Zhou Xu, Mingchang Li, Jian Wang
Ischemia/reperfusion (I/R) injuries commonly lead to breakdown of the blood-brain barrier (BBB).Restoration of the BBB can relieve neurologic damage caused by I/R injuries. The Hippo/YAP signaling pathway mediates cell proliferation, regulated cell death, and differentiation in various organisms and has been shown to participate in the restoration of the heart after I/R. In this study, we investigated whether the Hippo/YAP pathway plays a role in I/R injury in brain, especially in regard to I/R-induced BBB breakdown...
August 6, 2018: Behavioural Brain Research
Jia-Peng Wu, Xue-Zhi Li, Ying Wang, Lin Ma, Tai-Wan Yao, Yong-Yue Zhang, Fei Long
OBJECTIVE: To observe the effect of electroacupuncture (EA) and EA combined with intracerebral injection of vascular endothelial growth factor (VEGF) on endoplasmic reticulum stress (ERS) related proteins and genes as activating transcription factor (ATF 6), inositol requiring enzyme-1 (IRE 1), CCAAT/enhancer binding protein homologous protein (CHOP), X box-binding protein-1 (XBP 1) of cerebral ischemia reperfusion injury (CIRI) rats, so as to study its repair effect for CIRI. METHODS: Forty male SD rats were equally and randomly divided into 5 groups: sham operation, model, EA, VEGF and EA+VEGF groups ( n =8)...
June 25, 2018: Zhen Ci Yan Jiu, Acupuncture Research
Ji Hye Hwang, Vinoth R Kumar, Seok Yong Kang, Hyo Won Jung, Yong-Ki Park
OBJECTIVE: To investigate the effects of the flower buds extract of Tussilago farfara Linné (Farfarae Flos; FF) on focal cerebral ischemia through regulation of inflammatory responses in activated microglia. METHODS: Brain ischemia was induced in Sprague-Dawley rats by a transient middle cerebral artery occlusion (tMCAO) for 90 min and reperfusion for 24 h. Twenty rats were randomly divided into 4 groups (n=5 per group): normal, tMCAO-induced ischemic control, tMCAO plus FF extract 300 mg/kg-treated, and tMCAO plus MK-801 1 mg/kg-treated as reference drug...
August 8, 2018: Chinese Journal of Integrative Medicine
Shuzhen Zhu, Xiaoya Gao, Kaibin Huang, Yong Gu, Yafang Hu, Yongming Wu, Zhong Ji, Qing Wang, Suyue Pan
Glibenclamide (GBC) is an antidiabetic drug that is in a class of medications known as sulfonylureas, which play critical roles in attenuating brain edema and reducing mortality in ischemic stroke patients. Therapeutic hypothermia (TH) is another robust neuroprotectant that prevents brain swelling and improves the neurological outcomes of stroke patients. However, whether the combination of GBC and TH can be used as a reliable neuroprotectant in ischemic stroke remains largely unknown. We used the middle cerebral artery occlusion (MCAO) rat model as well as oxygen and glucose deprivation-reoxygenation (OGD/R) endothelial cells as ischemic stroke models to investigate the efficacy and mechanisms of treating ischemic stroke with the combination of GBC and TH...
August 2018: Aging and Disease
Tauheed Ishrat, Sahar Soliman, Wael Eldahshan, Bindu Pillai, Adviye Ergul, Susan C Fagan
The pro-survival effect of VEGF-B has been documented in different in vivo and in vitro models. We have previously shown an enhanced VEGF-B expression in response to candesartan treatment after focal cerebral ischemia. In this study, we aimed to silence VEGF-B expression to assess its contribution to candesartan's benefit on stroke outcome. Silencing VEGF-B expression was achieved by bilateral intracerebroventricular injections of lentiviral particles containing short hairpin RNA (shRNA) against VEGF-B. Two weeks after lentiviral injections, rats were subjected to either 90 min or 3 h of middle cerebral artery occlusion (MCAO) and randomized to intravenous candesartan (1 mg/kg) or saline at reperfusion...
August 7, 2018: Neurochemical Research
Feng Qin, Yang Zhao, Wei Shang, Zhi-Ming Zhao, Xin Qian, Bei-Bei Zhang, Hui Cai
Studies have shown that the inflammatory activation of miroglia (MG) and nuclear factor kappa B ( NF-κB ) play a dominant role in inflammatory response. Previous studies have shown that sinomenine, an anti-inflammatory agent extracted from Sinomenium acutum, can directly protect neurons against cerebral ischemia injury. However, there are no reports on its effect on ischemia/reperfusion-induced inflammatory activation of MG. In the present study, an in vitro ischemia/reperfusion model was developed with mouse BV-2 microglia cells, a model of oxygen-glucose deprivation/reperfusion (OGD/R), and the inhibitory effect of sinomenine pretreatment on inflammatory activation was confirmed through measurement of inflammatory indicators...
July 30, 2018: Cellular and Molecular Biology
Takatsugu Abe, Kuniyasu Niizuma, Atsushi Kanoke, Daisuke Saigusa, Ritsumi Saito, Akira Uruno, Miki Fujimura, Masayuki Yamamoto, Teiji Tominaga
We performed metabolomic analyses of mouse brain using a transient middle cerebral artery occlusion (tMCAO) model with Matrix Assisted Laser Desorption/Ionization (MALDI)-mass spectrometry imaging (MSI) to reveal metabolite changes after cerebral ischemia. We selected and analyzed three metabolites, namely creatine (Cr), phosphocreatine (P-Cr), and ceramides (Cer), because these metabolites contribute to cell life and death. Eight-week-old male C57BL/6J mice were subjected to tMCAO via the intraluminal blockade of the middle cerebral artery (MCA) and reperfusion 60 min after the induction of ischemia...
August 3, 2018: Neurologia Medico-chirurgica
Nuo Li, Sina Qin, Lu Xie, Tao Qin, Yegui Yang, Wei Fang, Meng-Hua Chen
BACKGROUND/AIMS: The anti-apoptotic effect of an increase in the extracellular concentration of potassium ([K+]) has been confirmed in vitro. However, it is not yet known whether elevated serum [K+] exerts a cerebroprotective effect in vivo. In this study, we aimed to explore the effect of elevated serum [K+] in a rat model of middle cerebral artery occlusion and reperfusion (MCAO/R). METHODS: Rats subjected to 90-min MCAO received 2.5% KCL, 1.25% KCL, or a normal saline solution at a dose of 3...
August 3, 2018: Cellular Physiology and Biochemistry
Alicia Aliena-Valero, Mikahela A López-Morales, María C Burguete, María Castelló-Ruiz, Teresa Jover-Mengual, David Hervás, Germán Torregrosa, Enrique C Leira, Ángel Chamorro, Juan B Salom
Preclinical and clinical studies support a promising, albeit not definitive, neuroprotective effect of emergent uric acid (UA) administration in ischemic stroke. We assessed the effects of UA in an ischemic stroke model relevant to the current treatment paradigm of mechanical thrombectomy within the STAIR/RIGOR recommendations. A cohort of male and female Wistar rats was subjected to ischemic stroke with mechanical recanalization under physiological monitoring. The effects of transient middle cerebral artery occlusion (tMCAO) with adjunctive UA (IV, 16 mg/kg) or vehicle treatment were assessed at 24 h and 7 days...
August 1, 2018: Neuroscience
Ting Zhang, Chang-Yan Li, Jing-Jing Jia, Jie-Shan Chi, Da Zhou, Jian-Zhou Li, Xiao-Ma Liu, Jun Zhang, Li Yi
Ischemia/reperfusion is known to greatly increase oxidative stress in the penumbra, which results in brain damage. Integrin αvβ3 is selectively up-regulated with ischemic injury to the brain and remains elevated throughout reperfusion. We determined whether or not a new compound biotinylated-LXW7-ceria nanoparticle (CeNP) (bLXW7-CeNP) plays a role in brain protection in the rat model of middle cerebral artery occlusion/reperfusion and shows better effects than CeNPs alone in improving the outcomes of focal oxidative stress and apoptosis more effectively...
February 2018: Current medical science
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