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cerebral ischemia-reperfusion

Y-J Zhao, Y Nai, S-Y Li, Y-H Zheng
OBJECTIVE: To investigate the effect of retigabine on the blood-brain barrier permeability in rats with cerebral ischemia-reperfusion and its mechanism. MATERIALS AND METHODS: A total of 90 Sprague-Dawley (SD) rats were selected to prepare a rat model of focal cerebral ischemia-reperfusion. The blood flow changes were detected using a laser Doppler flow meter, the percentage of the cerebral infarction volume was measured by means of the triphenyl tetrazolium chloride (TTC) staining, the effect of retigabine on the permeability of the blood-brain barrier after cerebral ischemia-reperfusion was examined via Evans blue (EB) staining, and the state of tight junctions between endothelial cells was determined via the transmission electron microscopy (TEM) technique...
December 2018: European Review for Medical and Pharmacological Sciences
Hui-Jie Wang, Jia-Yi Wei, Dong-Xin Liu, Shi-Fang Zhuang, Yuan Li, Hui Liu, Meng Ban, Wen-Gang Fang, Liu Cao, Wei-Dong Zhao, Yu-Hua Chen
Ischemic strokes often result in cerebral injury due to ischemia/reperfusion (I/R). Although the local inflammatory responses are known to play a primary role in the brain I/R injury, the underlying mechanism remains unclear. In the current study, we investigated the effect of brain endothelial Atg7 (autophagy related 7) depletion in the acute brain injury induced by ischemia and reperfusion. Endothelial knockout of Atg7 in mice (Atg7 eKO) was found to significantly attenuate both the infarct volume and the neurological defects induced by I/R when compared to the controls...
2018: Frontiers in Neurology
Qingfeng Xie, Jingyan Cheng, Guoyuan Pan, Shamin Wu, Quan Hu, Haoming Jiang, Yangyang Wang, Jianrong Xiong, Qiongyi Pang, Xiang Chen
Dendritic and synaptic plasticity in the penumbra are important processes and are considered to be therapeutic targets of ischemic stroke. Treadmill exercise is known to be a beneficial treatment following stroke. However, its effects and potential mechanism in promoting dendritic and synaptic plasticity remain unknown. We have previously demonstrated that the caveolin-1/VEGF signaling pathway plays a positive role in angiogenesis and neurogenesis. Here, we further investigated the effects of treadmill exercise on promoting dendritic and synaptic plasticity in the penumbra and whether they involve the caveolin-1/VEGF signaling pathway...
December 12, 2018: Experimental Neurology
Hyunjung Kim, Ji Hyeon Ahn, Minah Song, Dae Won Kim, Tae-Kyeong Lee, Jae-Chul Lee, Young-Myeong Kim, Jong-Dai Kim, Jun Hwi Cho, In Koo Hwang, Bing Chun Yan, Moo-Ho Won, Joon Ha Park
Fucoidan is a sulfated polysaccharide derived from brown algae and possesses various beneficial activities, including antioxidant property. Previous studies have shown that fucoidan displays protective effect against ischemia-reperfusion injury in some organs. However, few studies have been reported regarding the protective effect of fucoidan against transient cerebral ischemic insults and its related mechanisms. Therefore, in this study, we examined the neuroprotective effect of fucoidan against transient global cerebral ischemia (tGCI), as well as underlying its mechanism using a gerbil model of tGCI which shows a loss of pyramidal neurons in the hippocampal cornu ammonis 1 (CA1) area after 5 min of tGCI...
January 2019: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Shimaa K Mohamed, Amany A E Ahmed, Engy M El Morsy, Shahira Nofal
AIMS: Cerebral ischemia reperfusion (I/R) is a neurovascular disease leading to cerebral damage. It was found that postmenopausal women are liable to more dangerous effects than men at same age in stroke. The objective of this study is to investigate the neuroprotective effect of zeranol against cerebral ischemia reperfusion in ovariectomized rats. MAIN METHODS: 36 female wistar rats divided in to 3 groups: sham group, I/R group (where I/R was induced 7 weeks after ovariectomy), zeranol group (0...
December 11, 2018: Life Sciences
Yue Zhuo, Jun Zhuo
Ischemia-reperfusion injury (IRI) occurs when blood supply returns to tissue after interruption, which is associated with life-threatening inflammatory response. Tranilast is a widely used anti-allergic agent in the treatment against bronchial asthma and keloid. To study the function of tranilast, we used IRI rat model. The brain tissues of IRI rats with or without tranilast treatment were collected. Neuronal apoptosis in the brain was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling assay, and pro-inflammatory cytokine levels were measured by qRT-PCR and enzyme-linked immunosorbent assay...
December 10, 2018: Clinical and Translational Science
Yanyan Shi, Xiao-Hong Peng, Xia Li, Gao-Ping Luo, Ming-Fu Wu
Neuroprotective effects of dexmedetomidine (Dex) have been reported in various models of brain injury. However, to our knowledge, the neuroprotective mechanism of Dex pretreatment in rats remains unknown. The aim of the present study was to detect the expression of the α2A adrenergic receptor (ADRA2A) in focal ischemic brain tissues and to investigate the protective role and corresponding mechanism of Dex pretreatment in cerebral ischemia in rats. A hypoxia/reoxygenation (H/R) cell model in primary cultured astrocytes and a focal cerebral ischemia/reperfusion (I/R) model in adult rats were used...
December 2018: Experimental and Therapeutic Medicine
Yu Zhong, Chao Yu, Wenyi Qin
Recently, long non-coding RNAs (lncRNAs) are considered as critical regulators in pathogenesis progression of cerebral ischemia. In present study, lncRNA-small nucleolar RNA host gene 14 (SNHG14) was found upregulated in middle cerebral artery occlusion/reperfusion (MCAO/R) treated brain tissues and oxygen-glucose deprivation and reoxygenation (OGD/R) treated PC-12 cells. Interference of SNHG14 by shRNA vector enhanced neuron survival and suppressed inflammation in response to OGD/R insult. SNHG14 positively regulated the expression of Rho-associated coiled-coil-containing protein kinase 1 (ROCK1) via acting as a sponge of microRNA (miR)-136-5p...
December 14, 2018: Cancer Gene Therapy
Weijie Xie, Ping Zhou, Yifan Sun, Xiangbao Meng, Ziru Dai, Guibo Sun, Xiaobo Sun
Cerebral ischemia-reperfusion is a complicated pathological process. The injury and cascade reactions caused by cerebral ischemia and reperfusion are characterized by high mortality, high recurrence, and high disability. However, only a limited number of antithrombotic drugs, such as recombinant tissue plasminogen activator (r-TPA), aspirin, and heparin, are currently available for ischemic stroke, and its safety concerns is inevitable which associated with reperfusion injury and hemorrhage. Therefore, it is necessary to further explore and examine some potential neuroprotective agents with treatment for cerebral ischemia and reperfusion injury to reduce safety concerns caused by antithrombotic drugs in ischemic stroke...
December 12, 2018: Cells
Feng Tan, Jian Wang, Jing Xian Liu, Chen Wang, Miaodan Li, Yong Gu
Electroacupuncture (EA) may stimulate neurogenesis in animal models of ischemic stroke; however, the associated mechanisms are not clear. The present study aimed to evaluate the neurogenesis efficacy of EA on ischemic stroke and the underlying associated mechanisms. A model of middle cerebral artery occlusion (MCAO) was employed as the rat model of brain ischemia and reperfusion. EA treatment at the GV20 (Baihui) and GV14 (Dazhui) acupoints was conducted for 30 min daily following MCAO. Immunofluorescence was performed to measure the number of bromodeoxyuridine (BrdU)/nestin- or BrdU/doublecortin (DCX)-positive cells in the sham, MCAO and MCAO + EA groups...
December 2018: Experimental and Therapeutic Medicine
Jun-Jie Wang, Jun-De Zhu, Xian-Hu Zhang, Ting-Ting Long, Guo Ge, Yan Yu
As an inhibitor of the Notch signaling pathway, N-[N-(3,5-difluorohenacetyl)-l-alanyl]-S-phenylglycine tert-butyl ester (DAPT) may protect brain tissue from serious ischemic injury. This study aimed to explore neuroprotection by DAPT after cerebral ischemia/reperfusion (I/R) injury. DAPT was intraperitoneally injected 3 hours before the establishment of a focal cerebral I/R model in the right middle cerebral artery of obstructed mice. Longa scores were used to assess neurological changes of mice. Nissl staining and TdT-mediated dUTP-biotin nick-end labeling staining were used to examine neuronal damage and cell apoptosis in the right prefrontal cortex, while immunofluorescence staining was used to detect glial fibrillary acidic protein- and Notch1-positive cells...
March 2019: Neural Regeneration Research
Rong Huang, Juan Ma, Bo Niu, Jin Li, Jian Chang, Yanrui Zhang, Peng Liu, Xinping Luan
Ischemic stroke is one of the leading causes of death and disability globally and has been regarded as a major public health problem. Understanding the mechanism of ischemia/reperfusion (I/R)-induced oxidative stress injury may provide new treatment for ischemic stroke. Kelch-like ECH-associated protein 1 (Keap1)/ NF-E2-related factor 2 (Nrf2)/ antioxidant response elements (ARE) signaling pathway has been considered to be the major cellular defense against oxidative stress. In the present study, our objective is to evaluate the molecular mechanism of miR-34b/Keap1 in modulating focal cerebral I/R induced oxidative injury...
January 2019: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
Bingjin Ou, Wei Tao, Songbai Yang, Jiateng Feng, Jinfeng Wang, Tong Yang, Hanyu Wu, Yaguang Huang, Lingjing Tan, Weifeng Huang, Zhitao Feng, Zhigang Mei
Geum japonicum Thunb. var. chinense (GJ) is a type of wild vegetable found in China and other Asian countries; it has been reported that its extracts possess a neuroprotective effect against cerebral ischemia reperfusion (CIR) injury. The aim of this study is to explore the effect GJ extracts on transient focal CIR injury and neurons apoptosis and to clarify its possible underlying mechanisms in vivo . Our results indicated that pretreatment with GJ extracts significantly ameliorated the infarct volume, decreased neurological deficits, lessened neural cells apoptosis, downregulated GFAP activity level, and increased surviving neurons...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Ying Zhang, Longhui Ma, Changhong Ren, Kaiyin Liu, Xin Tian, Di Wu, Yuchuan Ding, Junfa Li, Cesar V Borlongan, Xunming Ji
Remote ischemic postconditioning (RIPC) is a promising neuroprotective strategy for ischemic stroke. Here, we employed a focal ischemic stroke mouse model to test the hypothesis that poststroke collateral circulation as a potent mechanism of action underlying the therapeutic effects of immediate RIPC. During reperfusion of cerebral ischemia, the mice were randomly assigned to receive RIPC, granulocyte colony-stimulating factor (G-CSF) as a positive control, or no treatment. At 24 hr, we found RIPC and G-CSF increased monocytes/macrophages in the dorsal brain surface and in the spleen, coupled with enhanced leptomeningeal collateral flow compared with nontreatment group...
December 10, 2018: Journal of Cellular Physiology
Xiaohua Tan, Hongmei Zhu, Qianyu Tao, Lisha Guo, Tianfang Jiang, Le Xu, Ruo Yang, Xiayu Wei, Jin Wu, Xiaokun Li, Jin-San Zhang
Ischemia-reperfusion (I/R) is a common cause of acute kidney injury (AKI), which is associated with high mortality and poor outcomes. Autophagy plays important roles in the homeostasis of renal tubular cells (RTCs) and is implicated in the pathogenesis of AKI, although its role in the process is complex and controversial. Fibroblast growth factor 10 (FGF10), a multifunctional FGF family member, was reported to exert protective effect against cerebral ischemia injury and myocardial damage. Whether FGF10 has similar beneficial effect, and if so whether autophagy is associated with the potential protective activity against AKI has not been investigated...
2018: Frontiers in Genetics
Qiong Yu, Li Li, Wei-Min Liang
Astrocytes, the major component of blood-brain barriers, have presented paradoxical profiles after cerebral ischemia and reperfusion in vivo and in vitro. Our previous study showed that sevoflurane preconditioning improved the integrity of blood-brain barriers after ischemia and reperfusion injury in rats. This led us to investigate the effects of sevoflurane preconditioning on the astrocytic dynamics in ischemia and reperfusion rats, in order to explore astrocytic cell-based mechanisms of sevoflurane preconditioning...
February 2019: Neural Regeneration Research
Guixing Zhang, Mingyue Ge, Ziwei Han, Sheng Wang, Jiangwen Yin, Li Peng, Feng Xu, Qingtong Zhang, Zhigang Dai, Liping Xie, Yan Li, Junqiang Si, Ketao Ma
AIM: The Wnt/β-catenin signaling pathway plays an important role in ischemia-reperfusion(I/R) injury, and the transforming growth factor(TGF)-β/Smad signaling pathway participates in the neuroprotection effect induced by isoflurane(ISO) postconditioning. In this study, we aimed to explore the role of the Wnt/|[beta]|-catenin β-catenin signaling pathway in the neuroprotection effect induced by ISO postconditioning, and investigate the interaction of Wnt/β-catenin and TGF-β/Smad signaling pathway in this neuroprotection effect...
December 5, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
L Zhai, J Wang, Y Q Ji, T T Wang, M Liu, Y L Guo
Objective: To investigate the effect and mechanisms of picroside Ⅱ on the brain tissue after cerebral ischemia reperfusion(I/R) in rats. Methods: The middle cerebral artery occlusion(MCAO) rat model was established by inserting a monofilament into middle cerebral artery. The experimental rats were treated by injecting picroside Ⅱ intraperitoneally. The modified neurological severity score (mNSS) and body weight were determined before modeling and after reperfusion of 22 h. The cerebral infarct volume was measured by TTC staining and the cerebral water content was measured in rats...
December 4, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
Fatemeh Zare Mehrjerdi, Ali Shoshtari, Fahimeh Mohseni, Hossein Khastar, Pirasteh Norozi, Yasin Asadi, Masoumeh Dadkhah, Mehdi Khaksari
Objectives: According to recent the findings, sulfur dioxide (SO2 ) is produced by the cardiovascular system, influencing some major biological processes. Based on previous research, SO2 exhibits antioxidant effects and inhibits apoptosis following cardiac ischemia/reperfusion. Therefore, the objective of the current study was to examine the neuroprotective impact of SO2 following global cerebral ischemia/reperfusion (I/R). Materials and Methods: Forty-eight male Wistar rats that weighed 260-300 g, were randomly allocated into 4 groups: sham group (n=12), I/R group (n=12), and I/R+SO2 groups (NaHSO3 and Na2 SO3 ; 1:3 ratio; 5 and 10 µg/kg, respectively; for 3 days, n=12)...
October 2018: Iranian Journal of Basic Medical Sciences
Lingling Zhang, Jianzong Chen
Polygonum multiflorum Thunb. (PM), a traditional Chinese medicinal herb, has been widely used in the Orient as a tonic and antiaging agent. 2,3,5,4'-Tetrahydroxystilbene-2- O - β -D-glucoside (TSG, C20 H22 O9 , FW = 406.38928) is one of the active components extracted from PM. TSG is an antioxidant agent, which exhibits remarkable antioxidative activities in vivo and in vitro. The antioxidant effect of TSG is achieved by its radical-scavenging effects. TSG can inhibit apoptosis and protect neuronal cells against injury through multifunctional cytoprotective pathways...
2018: Oxidative Medicine and Cellular Longevity
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