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https://www.readbyqxmd.com/read/30336307/next-generation-microrna-sequencing-to-identify-coronary-artery-disease-patients-at-risk-of-recurrent-myocardial-infarction
#1
Sri H Kanuri, Joseph Ipe, Kameel Kassab, Hongyu Gao, Yunlong Liu, Todd C Skaar, Rolf P Kreutz
BACKGROUND AND AIMS: Variation in micro-RNA (miRNA) levels in blood has been associated with alterations of physiological functions of the cardiovascular system. Circulating miRNA have the potential to become reliable biomarkers for risk stratification and early detection of cardiovascular events. Recurrent thrombotic events in patients with established coronary artery disease (CAD) demonstrate the need for personalized approaches to secondary prevention, especially in light of recent novel treatment approaches...
October 3, 2018: Atherosclerosis
https://www.readbyqxmd.com/read/30336279/bta-mir-130a-b-regulates-preadipocyte-differentiation-by-targeting-pparg-and-cyp2u1-in-beef-cattle
#2
Xueyao Ma, Dawei Wei, Gong Cheng, Shijun Li, Li Wang, Yaning Wang, Xiaoyu Wang, Song Zhang, Hongbao Wang, Linsen Zan
Deposition of intramuscular fat (IMF) is one of the most important traits for the evaluation of beef carcass quality grade. MicroRNA (miRNA) is an endogenous non-coding RNA that can play a role in the post-transcriptional regulation of mammalian preadipocyte differentiation. Previously, we identified that bta-miR-130a regulates milk fat biosynthesis by targeting PPARG mRNA. However, the role of miR-130 in the regulation of bovine adipocyte differentiation remains unknown. In this study, we found that overexpression of bta-miR-130a/b led to significantly decreased cellular triacylglycerol (TAG) levels during adipogenesis process as well as reduced lipid droplet formation...
October 15, 2018: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/30323832/identification-of-mir-200c-and-mir141-mediated-lncrna-mrna-crosstalks-in-muscle-invasive-bladder-cancer-subtypes
#3
Guojun Liu, Zihao Chen, Irina G Danilova, Mikhail A Bolkov, Irina A Tuzankina, Guoqing Liu
Basal and luminal subtypes of muscle-invasive bladder cancer (MIBC) have distinct molecular profiles and heterogeneous clinical behaviors. The interactions between mRNAs and lncRNAs, which might be regulated by miRNAs, have crucial roles in many cancers. However, the miRNA-dependent crosstalk between lncRNA and mRNA in specific MIBC subtypes still remains unclear. In this study, we first classified MIBC into two conservative subtypes using miRNA, mRNA and lncRNA expression data derived from The Cancer Genome Atlas...
2018: Frontiers in Genetics
https://www.readbyqxmd.com/read/30323063/microrna-664-5p-promotes-myoblast-proliferation-and-inhibits-myoblast-differentiation-by-targeting-serum-response-factor-and-wnt1
#4
Rui Cai, Naren Qimuge, Meilin Ma, Yingqian Wang, Guorong Tang, Que Zhang, Yunmei Sun, Xiaochang Chen, Taiyong Yu, Wuzi Dong, Gongshe Yang, Weijun Pang
MicroRNAs (miRNAs) are non-coding RNAs that regulate gene expression at the post-transcriptional level and are involved in the regulation of the formation, maintenance, and function of skeletal muscle. Using miRNA sequencing and bioinformatics analysis, we previously found that the miRNA miR-664-5p is significantly differentially expressed in longissimus dorsi muscles of Rongchang pigs. However, the molecular mechanism by which miR-664-5p regulates myogenesis remains unclear. In this study, using flow cytometry, 5-ethynyl-2'-deoxyuridine (EdU) staining, and cell count and immunofluorescent assays, we found that cell-transfected miR-664-5p mimics greatly promoted proliferation of C2C12 mouse myoblasts by increasing the proportion of cells in the S- and G2-phases and up-regulating the expression of cell cycle genes...
October 15, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/30322977/micrornas-regulate-survival-in-oxygen-deprived-environments
#5
Simon G English, Hanane Hadj-Moussa, Kenneth B Storey
Some animals must endure prolonged periods of oxygen deprivation to survive. One such extreme model is the Northern Crayfish ( Orconectes virilis ), that regularly survives year-round hypoxic and anoxic stresses in its warm stagnant summer waters and in its cold, ice-locked winter waters. To elucidate the molecular underpinnings of anoxia-resistance in this natural model, we surveyed the expression profiles of 76 highly-conserved microRNAs in crayfish hepatopancreas and tail muscle from normoxic, acute 2hr anoxia, and chronic 20hr anoxia exposures...
October 15, 2018: Journal of Experimental Biology
https://www.readbyqxmd.com/read/30322759/microrna-206-downregulates-connexin43-in-cardiomyocytes-to-induce-cardiac-arrhythmias-in-a-transgenic-mouse-model
#6
Yan Jin, Tian-Yi Zhou, Jia-Ning Cao, Qiu-Ting Feng, Ya-Jing Fu, Xin Xu, Cheng-Jian Yang
BACKGROUND: MicroRNAs (miRNAs) are critical modulators of various physiological and pathological processes, but their role in cardiac arrhythmias remains yet to be completely understood. Connexin43 (Cx43) is an important cardiac gap junction protein and a potential target of miR-206, and downregulation of Cx43 induces ventricular tachyarrhythmias. METHODS: We investigated the effects of miR-206 overexpression on the adult mouse heart and in cardiac arrhythmias. Luciferase activity assay was employed to validate Cx43 as a direct target of miR-206...
October 4, 2018: Heart, Lung & Circulation
https://www.readbyqxmd.com/read/30322616/mir-448-3p-controls-intracranial-aneurysm-by-regulating-klf5-expression
#7
Jian-Zhong Zhang, Dan Chen, Li-Quan Lv, Zheng Xu, Yi-Ming Li, Jun-Yu Wang, Kai-Wei Han, Ming-Kun Yu, Cheng-Guang Huang, Li-Jun Hou
microRNAs (miRNAs) control several processes known to be involved in progression of aneurysm. Here, intracranial aneurysms (IAs) were surgically induced in Sprague-Dawley rats, and we found that miR-448-3p was downregulated and KLF5 was upregulated in IA rats. We identified Klf5 as a direct target of miR-448-3p in smooth muscle cells (SMCs). In addition, aneurysms size and the lumen area of the aneurysms were smaller 4 weeks after IA induction in the miR-448-3p-treated group. miR-448-3p treatment protected the wall thickness ratio and suppressed macrophage infiltration after IA induction...
October 12, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/30317561/mir-208b-regulates-cell-cycle-and-promotes-skeletal-muscle-cell-proliferation-by-targeting-cdkn1a
#8
Jian Wang, Chengchuang Song, Xiukai Cao, Hui Li, Hanfang Cai, Yilei Ma, Yongzhen Huang, Xianyong Lan, Chuzhao Lei, Yun Ma, Yueyu Bai, Fengpeng Lin, Hong Chen
Skeletal muscle is the most abundant tissue in the body. The development of skeletal muscle cell is complex and affected by many factors. A sea of microRNAs (miRNAs) have been identified as critical regulators of myogenesis. MiR-208b, a muscle-specific miRNA, was reported to have a connection with fiber type determination. However, whether miR-208b has effect on proliferation of muscle cell was under ascertained. In our study, cyclin-dependent kinase inhibitor 1A (CDKN1A), which participates in cell cycle regulation, was predicted and then validated as one target gene of miR-208b...
October 14, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/30316681/circulating-microrna-plasma-profile-in-musk-myasthenia-gravis
#9
Liis Sabre, Jeffrey T Guptill, Melissa Russo, Vern C Juel, Janice M Massey, James F Howard, Lisa D Hobson-Webb, Anna Rostedt Punga
Muscle-specific tyrosine kinase antibody positive myasthenia gravis (MuSK+ MG) is an immunological subtype with distinctive pathogenic mechanisms and clinical features. The aim of this study was to analyze the circulating plasma microRNA profile of patients with MuSK+ MG. From the discovery cohort miR-210-3p, miR-324-3p and miR-328-3p were further analyzed in the validation cohort. We found a distinct plasma profile of miR-210-3p and miR-324-3p that were significantly decreased in MuSK+ MG patients compared to healthy controls (4...
October 6, 2018: Journal of Neuroimmunology
https://www.readbyqxmd.com/read/30315887/microrna-expression-profiles-in-upper-tract-urothelial-carcinoma-differentiate-tumor-grade-stage-and-survival-implications-for-clinical-decision-making
#10
Brendan M Browne, Kristian D Stensland, Chintan K Patel, Travis Sullivan, Eric J Burks, David Canes, Jay D Raman, Joshua Warrick, Kimberly M Reiger-Christ
OBJECTIVE: To evaluate microRNA biomarkers for upper tract urothelial carcinoma (UTUC) to improve risk stratification. METHODS: MicroRNA was isolated from 157 radical nephroureterectomy (RNU) specimens from two institutions. The relative expression of miRNA was examined for high grade versus low grade tumors as well as muscle invasive versus non-muscle invasive tumors. Recurrence free survival (RFS) and overall survival (OS) were also stratified using relative expression of specific miRNA...
October 10, 2018: Urology
https://www.readbyqxmd.com/read/30290263/up-and-downregulation-of-mature-mir-1587-function-by-modulating-its-g-quadruplex-structure-and-using-small-molecules
#11
Fangyuan Li, Wei Tan, Han Chen, Jiang Zhou, Ming Xu, Gu Yuan
Using bioinformatics analysis, we found some mature human miRNAs containing G-rich sequences with four G-tracts that had a high probability of forming G-quadruplex structures. Here, we chose G-rich miR-1587 as a model to characterize the function and regulation of miRNAs. Using electrospray ionization mass spectrometry, magnetic resonance imaging, circular dichroism spectrometry, we had confirmed that miR-1587 folded into a stable parallel G-quadruplex structure. By microarray, Q-RT-PCR and 3'UTR luciferase assay, TAGLN, an early marker of smooth muscle differentiation and tumor suppressor, was identified as a target gene of miR-1587, thus providing a direct target to study miR-1587 functions...
October 3, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/30287401/identification-and-comparative-analysis-of-the-mirna-expression-profiles-from-four-tissues-of-micropterus-salmoides-using-deep-sequencing
#12
Wangbao Gong, Yong Huang, Jun Xie, Guangjun Wang, Deguang Yu, Kai Zhang, Zhifei Li, Ermeng Yu, Jingjing Tian, Yun Zhu
In the present study, four small RNA libraries were constructed from an M. salmoides population and sequenced using deep sequencing technology. A total of 9,888,822; 8,519,365; 20,566,198; and 15,762,254 raw reads representing 666,097; 755,711; 978,923; and 840,175 unique sequences were obtained from the spleen, liver, kidney, and muscle libraries, respectively. As a result, 509 known miRNAs belonging to 143 families and 1157 novel miRNAs were identified. The miRNAs displayed diverse expression levels among the four libraries, among which most of the known miRNAs were expressed at higher levels than the novel miRNAs...
October 1, 2018: Genomics
https://www.readbyqxmd.com/read/30258406/diabetes-modulates-micrornas-29b-3p-29c-3p-199a-5p-and-532-3p-expression-in-muscle-possible-role-in-glut4-and-hk2-repression
#13
João V Esteves, Caio Y Yonamine, Danilo C Pinto-Junior, Frederico Gerlinger-Romero, Francisco J Enguita, Ubiratan F Machado
The reduced expression of solute carrier family 2, facilitated glucose transporter member 4 (GLUT4) and hexokinase-2 (HK2) in skeletal muscle participates in insulin resistance of diabetes mellitus (DM). MicroRNAs (miRNAs) have emerged as important modulators of mRNA/protein expression, but their role in DM is unclear. We investigated miRNAs hypothetically involved in GLUT4/HK2 expression in soleus muscle of type 1 diabetes-like rats. In silico analysis revealed 651 miRNAs predicted to regulate solute carrier family 2 member 4 ( Slc2a4 ) mRNA, several of them also predicted to regulate Hk2 mRNA, and 16 miRNAs were selected for quantification...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/30257973/microrna-regulation-of-the-hyper-proliferative-phenotype-of-vascular-smooth-muscle-cells-in-diabetes-mellitus
#14
Daniele Torella, Claudio Iaconetti, Roberta Tarallo, Fabiola Marino, Giorgio Giurato, Claudia Veneziano, Iolanda Aquila, Mariangela Scalise, Teresa Mancuso, Eleonora Cianflone, Chiara Valeriano, Pina Marotta, Laura Tammè, Carla Vicinanza, Ferdinando C Sasso, Domenico Cozzolino, Michele Torella, Alessandro Weisz, Ciro Indolfi
Harnessing the mechanisms underlying the exacerbated vascular remodelling in Diabetes Mellitus (DM) is pivotal to prevent the high toll of vascular diseases in DM patients. microRNAs (miRNA) regulate vascular smooth muscle cell (VSMC) phenotypic switch. However, miRNA modulation of the detrimental diabetic VSMC phenotype is underexplored. Streptozotocin-induced Type 1 Diabetes (T1DM) Wistar rats and T2DM Zucker rats underwent right carotid artery experimental angioplasty and global miRNA/mRNA expression profiling was obtained by RNA sequencing (RNA-Seq)...
September 26, 2018: Diabetes
https://www.readbyqxmd.com/read/30254626/micrornas-mediators-and-therapeutic-targets-to-airway-hyper-reactivity-after-respiratory-syncytial-virus-infection
#15
REVIEW
Shuwen Feng, Dongxin Zeng, Junwen Zheng, Dongchi Zhao
Respiratory syncytial virus (RSV) is the most important pathogen correlated to the first-time infant wheezing and later recurrence after its primary infection. RSV infection promotes the bronchial smooth muscle sensitivity to leukotrienes (LTs) in acute stage, causes the extensive inflammatory reaction and the aggregation of Th2-like cells during respiratory tract obstruction. Infants and young children infected with RSV exhibit an increased susceptibility to the exposure of exogenous allergens, easy to suffer from the recurrent wheezing, which prompts that the body is still in a state of inflammation or immunological bias...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/30254065/human-placental-exosomes-in-gestational-diabetes-mellitus-carry-a-specific-set-of-mirnas-associated-with-skeletal-muscle-insulin-sensitivity
#16
Soumyalekshmi Nair, Nanthini Jayabalan, Dominic Guanzon, Carlos Palma, Katherin Scholz-Romero, Omar Elfeky, Felipe Zuñiga, Valeska Ormazabal, Emilio Diaz, Gregory E Rice, Gregory Duncombe, Thomas Jansson, Harold David McIntyre, Martha Lappas, Carlos Salomon
There is increasing evidence that miRNAs, which are enriched in nanovesicles called exosomes, are important regulators of gene expression. When compared to normal pregnancies, gestational diabetes mellitus (GDM) is associated with skeletal muscle insulin resistance as well as increased levels of circulating placental exosomes. Here we investigated whether placental exosomes in GDM carry a specific set of miRNAs associated with skeletal muscle insulin sensitivity. Exosomes were isolated from chorionic villous (CV) explants from both women with Normal Glucose Tolerant (NGT) and GDM pregnancies...
September 25, 2018: Clinical Science (1979-)
https://www.readbyqxmd.com/read/30245975/urinary-markers-in-bladder-cancer-an-update
#17
REVIEW
Giorgio Santoni, Maria B Morelli, Consuelo Amantini, Nicola Battelli
Bladder cancer (BC) is ones of the most common cancer worldwide. It is classified in muscle invasive (MIBC) and muscle non-invasive (NMIBC) BC. NMIBCs frequently recur and progress to MIBCs with a reduced survival rate and frequent distant metastasis. BC detection require unpleasant and expensive cystoscopy and biopsy, which are often accompanied by several adverse effects. Thus, there is an urgent need to develop novel diagnostic methods for initial detection and surveillance in both MIBCs and NMIBCs. Multiple urine-based tests approved by FDA for BC detection and surveillance are commercially available...
2018: Frontiers in Oncology
https://www.readbyqxmd.com/read/30238981/laminar-shear-stress-provoked-cytoskeletal-changes-are-mediated-by-epigenetic-reprogramming-of-timp1-in-human-primary-smooth-muscle-cells
#18
Rodrigo A da Silva, Célio Jr da C Fernandes, Geórgia da S Feltran, Anderson M Gomes, Amanda Fantini de Camargo Andrade, Denise C Andia, Maikel P Peppelenbosch, Willian F Zambuzzi
Whereas endothelial responses to shear stress are well-characterized, the cell physiological effects of shear stress in smooth muscle cells (SMCs) remain largely obscure. As SMCs are directly challenged by shear stress after endothelial denuding injury following procedures such as angioplasty or endarterectomy, characterization of these responses represents an important scientific question. Hence we decided to contrast cytoskeletal reorganization, epigenetic reprogramming, signaling transduction, and changes in miRNA (miRs) profiles in primary human aortic smooth muscle cells (AoSMCs) between unstressed cells and cells exposed to shear stress...
September 21, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/30235878/guanidinoacetic-acid-regulates-myogenic-differentiation-and-muscle-growth-through-mir-133a-3p-and-mir-1a-3p-co-mediated-akt-mtor-s6k-signaling-pathway
#19
Yujie Wang, Jideng Ma, Wanling Qiu, Jinwei Zhang, Siyuan Feng, Xiankun Zhou, Xun Wang, Long Jin, Keren Long, Lingyan Liu, Weihang Xiao, Qianzi Tang, Li Zhu, Yanzhi Jiang, Xuewei Li, Mingzhou Li
Guanidinoacetic acid (GAA), an amino acid derivative that is endogenous to animal tissues including muscle and nerve, has been reported to enhance muscular performance. MicroRNA (miRNA) is a post-transcriptional regulator that plays a key role in nutrient-mediated myogenesis. However, the effects of GAA on myogenic differentiation and skeletal muscle growth, and the potential regulatory mechanisms of miRNA in these processes have not been elucidated. In this study, we investigated the effects of GAA on proliferation, differentiation, and growth in C2C12 cells and mice...
September 19, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/30231015/phenotype-of-vascular-smooth-muscle-cells-vsmcs-is-regulated-by-mir-29b-by-targeting-sirtuin-1
#20
Qian-Ru Sun, Xiong Zhang, Kun Fang
BACKGROUND Phenotypic switch of vascular smooth muscle cells (VSMCs) participates in the etiology of various vascular diseases. It has been proved that microRNAs (miRNAs) serve as crucial regulators of functions of VSMCs. This study aimed to discover how miR-29b regulates the transformation of VSMCs phenotypes in mice. MATERIAL AND METHODS Primary VSMCs of aorta in mice were cultured in DMEM medium. A series of experiments involving transfection of oligonucleotides in cultured VSMCs, quantitative reverse transcription PCR (qRT-PCR), luciferase reporter assay, and Western blotting analysis were performed in this study...
September 19, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
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