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miRNA muscle

Karolina A Chodkowska, Anna Ciecierska, Kinga Majchrzak, Piotr Ostaszewski, Tomasz Sadkowski
Gamma-oryzanol (GO) is a popular supplement for performance horses, dogs, and humans. Previous studies indicated that GO supplementation decreases creatine kinase activity and lactate level after exercise and may affect oxidative stress in Thoroughbred horses. GO may change genes expression in equine satellite cells (ESC). The purpose of this study was to evaluate the effect of GO on miRNA, gene expression, oxidative stress, and cell damage and viability in differentiating ESC pretreated with hydrogen peroxide (H₂O₂)...
December 2, 2018: Nutrients
Hongcai Cai, Yamin Li, Jean Damascene Niringiyumukiza, Ping Su, Wenpei Xiang
Circular RNA (circRNA) is a class of newly discovered noncoding RNA (ncRNA), presenting as a special covalent loop without a 5' cap or 3' tail. Multiple biological properties of circRNA have been revealed during the past decades, such as widespread expression, high conservation, cell-specificity, tissue-specificity and developmental stage-specific expression patterns, as well as resistance to RNase R digestion. CircRNA also exhibits diverse biological functions, including regulation of host genes, alternative splicing, miRNA sponges, protein traps and even protein synthesis...
December 1, 2018: Mechanisms of Ageing and Development
Jasmine Mikovic, Kate Sadler, Lauren Butchart, Sarah Voisin, Frederico Gerlinger-Romero, Paul Della Gatta, Miranda D Grounds, Séverine Lamon
MicroRNAs (miRNAs) undergo high levels of regulation in skeletal muscle development and control skeletal muscle mass, function and metabolism over the lifespan. More recently, the role of long non-coding RNAs (lncRNAs) in skeletal muscle regulation has started to emerge. Following up on our recent study describing the expression pattern and putative roles of 768 miRNAs in the quadriceps muscle of mice at early life stages, we used a high-throughput miRNA qPCR-based array to assess the expression of the same miRNAs in 28-month old male mouse quadriceps muscle...
2018: Frontiers in Genetics
Francesca Vacante, Laura Denby, Judith C Sluimer, Andrew H Baker
Noncoding RNAs (long noncoding RNAs and small RNAs) are emerging as critical modulators of phenotypic changes associated with physiological and pathological contexts in a variety of cardiovascular diseases (CVDs). Although it has been well established that hereditable genetic alterations and exposure to risk factors are crucial in the development of CVDs, other critical regulators of cell function impact on disease processes. Here we discuss noncoding RNAs have only recently been identified as key players involved in the progression of disease...
November 28, 2018: Vascular Pharmacology
Wendy J Bailey, John E Barnum, Zoltan Erdos, Lisa LaFranco-Scheuch, Pamela Lane, Katerina Vlasakova, Frank D Sistare, Warren E Glaab
Liver and skeletal muscle specific microRNAs (miRNAs) are currently being evaluated as novel plasma biomarkers that may out-perform or add value to the conventional liver injury biomarkers alanine aminotransferase (ALT) and aspartate aminotransferase (AST), and to the skeletal muscle injury biomarkers AST and creatine kinase (CK). A comprehensive evaluation was conducted to assess the relative performance of these miRNAs to detect and distinguish liver from muscle tissue injury. The performance of miR-122 and miR-192 for liver and miR-1, miR-133a, miR-133b, and miR-206 for skeletal muscle was compared to 10 enzymatic or protein biomarkers across 27 compounds causing specific types of tissue injury in rat...
November 28, 2018: Toxicological Sciences: An Official Journal of the Society of Toxicology
Zhijin Liu, Cunyuan Li, Xiaoyue Li, Yang Yao, Wei Ni, Xiangyu Zhang, Yang Cao, Wureli Hazi, Dawei Wang, Renzhe Quan, Shuting Yu, Yuyu Wu, Songmin Niu, Yulong Cui, Shengwei Hu
Objectve: MicroRNAs are a class of endogenous small regulatory RNAs that regulate cell proliferation, differentiation and apoptosis. Recent studies on miRNAs are mainly focused on mice, human and pig. However, the studies on miRNAs in skeletal muscle of sheep are not comprehensive. Methods: RNA-seq technology was used to perform genomic analysis of miRNAs in prenatal and postnatal skeletal muscle of sheep. Targeted genes were predicted using miRanda software and miRNA-mRNA interactions were verified by quantitative Real-time PCR...
November 27, 2018: Asian-Australasian Journal of Animal Sciences
Liangming Chen, Rong Huang, Denghui Zhu, Cheng Yang, Libo He, Yongming Li, Lanjie Liao, Zuoyan Zhu, Yaping Wang
This research identified 169 known microRNAs (mirNA) mirNAs, 380 novel mirNAs, and 30,538 targets in 11 tissues (blood, brain, derma, gill, heart, intestine, kidney, liver, muscle, pronephros, and spleen) from grass carp Ctenopharyngodon idella with high-throughput sequencing (HTS). Transcripts per million (TPM) expression analysis detected 41 brain-enriched mirNAs (accounting for 61.19% of all tissue-enriched mirNAs). Real-time quantitative PCR (rt-qPCR) confirmed that 21 of 24 randomly selected tissue-enriched mirNAs from the TPM analysis were indeed tissue-enriched (P < 0...
November 24, 2018: Journal of Fish Biology
Ningzhao Wu, Tiantian Gu, Lu Lu, Zhengfeng Cao, Qianqian Song, Zhixiu Wang, Yang Zhang, Guobin Chang, Qi Xu, Guohong Chen
MicroRNA (miRNA)-1 and miRNA-133 are derived from the same bicistronic pairs with roles in skeletal muscle development. Many investigations have focused on the role of miRNA-1 and miRNA-133 in the regulation of skeletal muscle development in mammals and fish. However, the mechanisms of miRNA-1 and miRNA-133 underlying the differences in skeletal muscle development between different breeds are not well known. Our study found that the weights of body and breast at 42 days of age were greater in Cherry Valley ducks than in Putian ducks and the areas of breast muscle fibers increased with age; the areas of muscle fibers of Cherry Valley ducks were always greater than those of Putian ducks...
November 23, 2018: Journal of Cellular Physiology
Laura Nigi, Giuseppina Emanuela Grieco, Giuliana Ventriglia, Noemi Brusco, Francesca Mancarella, Caterina Formichi, Francesco Dotta, Guido Sebastiani
The insulin signaling pathway is composed of a large number of molecules that positively or negatively modulate insulin specific signal transduction following its binding to the cognate receptor. Given the importance of the final effects of insulin signal transduction, it is conceivable that many regulators are needed in order to tightly control the metabolic or proliferative functional outputs. MicroRNAs (miRNAs) are small non-coding RNA molecules that negatively modulate gene expression through their specific binding within the 3'UTR sequence of messenger RNA (mRNA), thus causing mRNA decoy or translational inhibition...
November 22, 2018: International Journal of Molecular Sciences
F-P Li, D-Q Lin, L-Y Gao
OBJECTIVE: Atherosclerosis is a major risk factor for cardiovascular disease, but its mechanism of progression remained unclear. However, many long non-coding RNAs (lncRNAs) have recently been implicated in different processes for cardiovascular disease. In this study, we mainly focused on the role of lncRNA TUG1 in atherosclerosis. PATIENTS AND METHODS: qRT-PCR was used to detect the expression of lncRNA TUG1 in atherosclerosis patients and animal model. Moreover, the expression of TUG1 in vascular smooth muscle cell dysfunction model was also measured...
November 2018: European Review for Medical and Pharmacological Sciences
Claudia Ricci, Carlotta Marzocchi, Stefania Battistini
Amyotrophic lateral sclerosis (ALS) is an incurable and fatal disorder characterized by the progressive loss of motor neurons in the cerebral cortex, brain stem, and spinal cord. Sporadic ALS form accounts for the majority of patients, but in 1⁻13.5% of cases the disease is inherited. The diagnosis of ALS is mainly based on clinical assessment and electrophysiological examinations with a history of symptom progression and is then made with a significant delay from symptom onset. Thus, the identification of biomarkers specific for ALS could be of a fundamental importance in the clinical practice...
November 20, 2018: Cells
Chuang Li, Fang Qin, Mengmeng Xue, Yucong Lei, Fen Hu, Hui Xu, Guihong Sun, Tao Wang, Mingxiong Guo
Cytosolic free Ca2+ concentration is a key factor in pulmonary vasoconstriction and vascular remodeling of pulmonary artery smooth muscle cells (PASMC). These processes contribute to pulmonary arterial hypertension and are influenced by expression of calcium sensing receptor (CaSR). Although regulation of CaSR expression is precisely controlled, the contribution of microRNAs is incompletely understood. Here, we demonstrate that miR-429, miR-424-5p, miR-200b-3p, and miR-200c-3p regulate CaSR by targeting specific 3'-UTR, suggesting that these miRNAs function as CaSR inhibitors in PASMC...
November 21, 2018: American Journal of Physiology. Cell Physiology
Ce Cui, Meiling Wen, Fang Zhou, Yunhui Zhao, Xiaoyan Yuan
Clinical utility of small-diameter vascular grafts is still challenging in blood vessel regeneration owing to thrombosis and intimal hyperplasia. To cope with the issues, modulation of gene expression via microRNAs (miRNAs) could be a feasible approach by rational regulating physiological activities of both vascular endothelial cells (VECs) and vascular smooth muscle cells (VSMCs). Our previous studies demonstrated that individually loaded miRNA-126 (miR-126) or miRNA-145 (miR-145) in the electrospun membranes showed the tendency to promote vascular regeneration...
November 21, 2018: Journal of Biomedical Materials Research. Part A
Jie Li, Gaofu Wang, Jing Jiang, Lin Fu, Peng Zhou, Hangxing Ren
MicroRNAs (miRNAs) have been reported that can regulate skeletal muscle growth and development. Previously, we demonstrated that miR-127-3p were differently expressed in skeletal muscle and muscle cells. However, the molecular mechanism of miR-127-3p regulation of skeletal myogenesis are not well elucidated. In this study, we transfected miR-127-3p into C2C12 cells, and found miR-127-3p induces myogenesis by targeting Vamp2 . Moreover, the regulatory mechanism of Vamp2 in myoblasts proliferation and differentiation was further confirmed...
2018: Animal Cells and Systems
Priscila S N De Oliveira, Luiz L Coutinho, Polyana C Tizioto, Aline S M Cesar, Gabriella B de Oliveira, Wellison J da S Diniz, Andressa O De Lima, James M Reecy, Gerson B Mourão, Adhemar Zerlotini, Luciana C A Regitano
Residual Feed Intake (RFI) is an economically relevant trait in beef cattle. Among the molecular regulatory mechanisms, microRNAs (miRNAs) are an important dimension in post-transcriptional regulation and have been associated with different biological pathways. Here, we performed differential miRNAs expression and weighted gene co-expression network analyses (WGCNA) to better understand the complex interactions between miRNAs and mRNAs expressed in bovine skeletal muscle and liver. MiRNA and mRNA expression data were obtained from Nelore steers that were genetically divergent for RFI (N = 10 [low RFI or feed efficient]; N = 10 [high RFI or feed inefficient])...
November 20, 2018: Scientific Reports
Kittipong Maneechotesuwan
The various roles of microRNAs (miRNAs) in the epigenetic regulation of human disease are gaining importance as areas of research, and a better understanding of these roles may identify targets for development of novel therapies for severe asthma. MiRNAs, a class of small non-coding RNAs that serve as post-transcriptional gene repressors, are recognized as critical components in regulating tissue homeostasis. Alteration in miRNA expression disrupts homeostasis and is an underlying mechanism for development of chronic respiratory diseases, including asthma...
November 16, 2018: Respiratory Investigation
Jaroslav Juracek, Michal Stanik, Petra Vesela, Lenka Radova, Jan Dolezel, Marek Svoboda, Ondrej Slaby
OBJECTIVES: Non-muscle-invasive bladder cancer (NMIBC) is a heterogeneous disease characterized by a high primary tumor recurrence rate. Current prognostic systems used for predicting recurrence in individual patients have limitations and do not consider the biological background of this tumor type. Our study aimed to find microRNAs (miRNAs) associated with NMIBC recurrence. METHODS: Seventy-eight NMIBC patients were prospectively enrolled and divided into exploratory and validation cohorts...
November 13, 2018: Urologic Oncology
Leah E Worton, Edith M Gardiner, Ronald Y Kwon, Leah M Downey, Brandon J Ausk, Steven D Bain, Ted S Gross
At sufficient dose, intramuscular injection of Botulinum toxin A causes muscle wasting that is physiologically consistent with surgical denervation and other types of neuromuscular dysfunction. The aim of this study was to clarify early molecular and micro-RNA alterations in skeletal muscle following Botulinum toxin A-induced muscle paralysis. Quadriceps were analyzed for changes in expression of micro- and messenger RNA and protein levels after a single injection of 0.4, 2 or 4U Botulinum toxin A (/100g body weight)...
2018: PloS One
Jeong Hyo Lee, Jeong-Woong Park, Kyung Soo Kang, Tae Sub Park
1. In the poultry industry, growth performance is important due to its effects on economic value. Much effort has been put forth to achieve introgression of specific genes and DNA markers related to muscle proliferation and differentiation in selective breeding approaches. 2. This study investigated the biological functions of the gene Forkhead Box O3 (Foxo3) during myogenic differentiation in chicken myoblast cells. Foxo3 was downregulated in primary chicken myoblast (pCM) cells by the piggyBac transposon-mediated miRNA knockdown (KD) system...
November 14, 2018: British Poultry Science
Orazio Fortunato, Cristina Borzi, Massimo Milione, Giovanni Centonze, Davide Conte, Mattia Boeri, Carla Verri, Massimo Moro, Federica Facchinetti, Francesca Andriani, Luca Roz, Laura Caleca, Veronica Huber, Agata Cova, Chiara Camisaschi, Chiara Castelli, Valeria Cancila, Claudio Tripodo, Ugo Pastorino, Gabriella Sozzi
miRNAs play a central role in the complex signaling network of cancer cells with the tumor microenvironment. Little is known on the origin of circulating miRNAs and their relationship with the tumor microenvironment in lung cancer. Here, we focused on the cellular source and relative contribution of different cell types to circulating miRNAs composing our risk classifier of lung cancer using in vitro/in vivo models and clinical samples. A cell-type specific expression pattern and topography of several miRNAs such as mir-145 in fibroblasts, mir-126 in endothelial cells, mir-133a in skeletal muscle cells was observed in normal and lung cancer tissues...
November 13, 2018: International Journal of Cancer. Journal International du Cancer
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