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Dermal fibroblast

Hauke Baumann, Magdalena Jahn, Alexander Muenchau, Michaela Trilck-Winkler, Katja Lohmann, Philip Seibler
Mutations in THAP1 (THAP domain-containing apoptosis-associated protein 1) cause a form of early-onset, isolated dystonia (DYT-THAP1, aka DYT6). Here, we describe the generation of eight human induced pluripotent stem cell (iPSC) lines of manifesting and non-manifesting carriers of the THAP1 mutations p.Lys158Asnfs*23 or p.Arg13His (each 4 lines). Dermal fibroblasts were reprogrammed using non-integrating Sendai virus. The iPSC lines were comprehensively characterized including expression analyses of pluripotency markers, the potential to differentiate into cells of all three germ layers, and stable karyotypes...
October 1, 2018: Stem Cell Research
Rachel J Jerrell, Mitchell J Leih, Aron Parekh
During the dermal wound healing process, the mechanical rigidity of the newly deposited extracellular matrix and transforming growth factor-β1 promote the transition of fibroblasts into myofibroblasts. Myofibroblasts generate large cellular forces that contract and remodel the extracellular matrix leading to scar formation. In contrast, myofibroblasts are not detected in fetal dermal wounds which are more compliant and contain less transforming growth factor-β1 than adult wounds. Instead, fetal fibroblasts orchestrate scarless healing of dermal wounds resulting in healed tissues that resemble uninjured dermis...
October 13, 2018: Wound Repair and Regeneration
Jeannie Devereaux, Kulmira Nurgali, Dimitrios Kiatos, Samy Sakkal, Vasso Apostolopoulos
Platelet-rich plasma is an autologous and safe blood product containing a high concentration of platelets and leucocytes. Platelets, growth factors, leucocytes and plasma are fundamental fibroblast proliferation agents. Leucocytes' plasticity, reparative qualities, cross-talk between cells and capacity to orchestrate diverse outcomes are receiving considerable research attention. Fibroblasts are able to migrate and proliferate into the tissue surrounding a wound and subsequently deposit granulation tissue, which minimises scarring...
November 2018: Maturitas
Soo Kim, Seul Ki Lee, Hyunjung Kim, Tae Min Kim
Induced pluripotent stem cell (iPSC)-derived mesenchymal stem cells (iMSCs) serve as a unique source for cell therapy. We investigated whether exosomes from iMSCs promote the proliferation of human keratinocytes (HaCaT) and human dermal fibroblasts (HDFs). iPSCs were established from human Wharton's jelly MSCs and were allowed to differentiate into iMSCs. Exosomes were collected from the culture supernatant of MSCs (MSC-exo) and iMSCs (iMSC-exo), and their characteristics were investigated. Both exosome types possessed basic characteristics of exosomes and were taken up by skin cells in vitro and in vivo...
October 11, 2018: International Journal of Molecular Sciences
E N Burns, M H Bordbari, M J Mienaltowski, V K Affolter, M V Barro, F Gianino, G Gianino, E Giulotto, T S Kalbfleisch, S A Katzman, M Lassaline, T Leeb, M Mack, E J Müller, J N MacLeod, B Ming-Whitfield, C R Alanis, T Raudsepp, E Scott, S Vig, H Zhou, J L Petersen, R R Bellone, C J Finno
The Functional Annotation of Animal Genomes (FAANG) project aims to identify genomic regulatory elements in both sexes across multiple stages of development in domesticated animals. This study represents the first stage of the FAANG project for the horse, Equus caballus. A biobank of 80 tissue samples, two cell lines and six body fluids was created from two adult Thoroughbred mares. Ante-mortem assessments included full physical examinations, lameness, ophthalmologic and neurologic evaluations. Complete blood counts and serum biochemistries were also performed...
October 11, 2018: Animal Genetics
Penghui Cheng, Jianjian Zhang, Jiaguo Huang, Qingqing Miao, Chenjie Xu, Kanyi Pu
Development of molecular probes for the detection of reactive oxygen and nitrogen species (RONS) is important for the pathology and diagnosis of diseases. Although an abnormally high RONS level has been identified in keloids - a benign dermal tumour developed after lesion, the ability of employing RONS probes for keloid detection has not yet been exploited. Herein, we report two near-infrared (NIR) fluorescent probes ( CyTF and CyBA ) that can specifically distinguish keloid fibroblasts from normal dermal fibroblasts...
August 14, 2018: Chemical Science
Dominika Wcisło-Dziadecka, Klaudia Simka, Agata Kaźmierczak, Celina Kruszniewska-Rajs, Joanna Gola, Beniamin Grabarek, Jolanta Hybiak, Catherine Grillon, Urszula Mazurek, Marek J Łos
BACKGROUND/AIMS: Psoriasis, an autoimmune diseases of the skin, characterized by patches of abnormal/inflammed skin, although not usually life-threatening, it causes severe discomfort, esthetic impairments, and may lead to impaired social functions and social withdrawal. Besides UV-phototherapy, various anti-inflammatory treatments are applied, depending on the severity of symptoms. In 2008, adalimumab (fully humanized human anti-TNF antibody) was launched for the treatment of psoriasis...
October 11, 2018: Cellular Physiology and Biochemistry
Toshio Morikawa, Akifumi Nagatomo, Kayako Kitazawa, Osamu Muraoka, Takashi Kikuchi, Takeshi Yamada, Reiko Tanaka, Kiyofumi Ninomiya
The seed oil of andiroba (Carapa guianensis, Meliaceae) was found to promote collagen synthesis in normal human dermal fibroblasts. To characterize the active constituents of this oil, the collagen synthesis-promoting activities of 10 principal limonoid constituents, gedunin (1), 6α-acetoxygedunin (2), 7-deacetoxy-7-oxogedunin (3), 7-deacetoxy-7α-hydroxygedunin (4), andirolide H (5), 6α-hydroxygedunin (6), methyl angolensate (7), 17β-hydroxyazadiradione (8), and carapanosides C (9) and R (10), were examined...
2018: Journal of Oleo Science
Yan Huang, Xiaoqiang Lü, Xiaoying Lü
The aim of this study was to screen and research key biological pathways and important microRNAs (miRNAs) involved in silver nanoparticles (SNPs) induced cytotoxicity using miRNA sequencing technology. First, the influences of five SNPs with different sizes (∼5, ∼20, ∼50, ∼100 and ∼200 nm), named as SNP-5, SNP-20, SNP-50, SNP-100, and SNP-200, on human dermal fibroblasts (HDFs) proliferation were evaluated by MTT assay after 4, 8, 12, 24, 48 and 72 h. miRNA sequencing technology was then utilized to screen differentially expressed miRNAs in SNPs treated HDFs for 4 h...
December 1, 2018: Journal of Biomedical Nanotechnology
Fatma Kübra Tombulturk, Tugba Soydas, Elif Yaprak Sarac, Matem Tuncdemir, Ender Coskunpinar, Erdal Polat, Serhat Sirekbasan, Gonul Kanigur-Sultuybek
AIMS: Lucilia sericata larvae have been successfully used on healing of wounds in the diabetics. However, the involvement of the extraction/secretion (ES) products of larvae in the treatment of diabetic wounds is still unknown. Activator protein-1 (AP-1) transcription, composed of c-jun and c-Fos proteins, has been shown to be the principal regulator of multiple MMP transcriptions under a variety of conditions, also in diabetic wounds. Specifically, MMP-2 and MMP-9's transcriptions are known to be modulated by AP-1...
October 9, 2018: Acta Diabetologica
Amandine André, Joanna Wdzieczak-Bakala, Alexis Kaatio Touré, Didier Stien, Véronique Eparvier
Glycation is a common non-enzymatic reaction between proteins and sugars, which gives rise in the human body to the formation of advanced glycation end products (AGEs). These modifications impacts both extra and intracellular proteins, leading to cells and tissues dysfunctions. In the skin, accumulation of AGEs leads to aesthetic consequences, wrinkles, dark spots and yellowish skin tone, as it can be seen in diabetic patients. Consequently, there is a growing dermatological interest to find compounds able to eliminate AGEs accumulated in skin...
October 4, 2018: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Kanav Khosla, Li Zhan, Aditya Bhati, Aiden Carley-Clopton, Mary Hagedorn, John C Bischof
Preventing ice formation during cryopreservation by vitrification has led to the successful storage and banking of numerous cellular and tissue-based biomaterials. In their breakthrough work, Peter Mazur's group achieved over 90% survival by using a laser warming technique for 100 µm mice oocytes that were cooled in 0.1 µL droplets with 2.3M CPA and extracellularly loaded India Ink (laser absorber). Laser warming can provide rapid and uniform warming rates to "outrun" damaging ice crystal growth...
October 9, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
Daniel David Stöbener, Anke Hoppensack, Johanna Scholz, Marie Weinhart
In this study, we introduce a platform to fabricate human dermal fibroblast (HDF), human aortic smooth muscle cell (HAoSMC) and human umbilical vein endothelial cell (HUVEC) sheets using thermoresponsive poly(glycidyl ether) coatings. Copolymer brushes based on glycidyl methyl ether (GME) and ethyl glycidyl ether (EGE) were self-assembled onto polystyrene (PS) culture substrates via the physical adsorption of a hydrophobic, photoreactive benzophenone anchor block based on the monomer 4-[2-(2,3-epoxypropoxy)ethoxy]benzophenone (EEBP)...
October 9, 2018: Soft Matter
Engi Ahmed, Caroline Sansac, Mathieu Fieldes, Anne Bergougnoux, Chloé Bourguignon, Joffrey Mianné, Cécile Arnould, Isabelle Vachier, Said Assou, Arnaud Bourdin, John De Vos
Primary Ciliary Dyskinesia (PCD) is a rare heterogeneous genetic disorder affecting motile cilia structure and function leading to lung disease. We generated induced pluripotent stem cells (iPSCs) from dermal fibroblasts of a female PCD patient carrying disease-causing variants in the CCDC40 gene. Reprogramming was performed with the human OSKM transcription factors using the Sendai-virus delivery system. The resulting transgene free iPSCs had normal karyotype, expressed pluripotency markers, could differentiate into the three germ layers in vivo and retained the disease-causing CCDC40 mutations...
September 28, 2018: Stem Cell Research
Cameron C Felty, Konstantinos Linos
Epithelioid fibrous histiocytoma (EFH) is a rare lesion believed to arise from dermal microvascular unit fibroblasts and dendritic histiocytes. EFH has long been considered a morphologic variant of benign fibrous histiocytoma (dermatofibroma), with prominent epithelioid cytomorphology that can mimic both vascular and melanocytic neoplasms. The molecular basis for the relationship between EFH and benign fibrous histiocytoma has remained largely unknown, with some authors suggesting that EFH represents an entity that is biologically distinct from benign fibrous histiocytoma...
October 4, 2018: American Journal of Dermatopathology
Wei He, Xiangqi Huang, Yudong Zheng, Yi Sun, Yajie Xie, Yansen Wang, Lina Yue
Bacterial cellulose (BC), with unique structure and properties, has attracted much attention in the biomedical field, especially in using as wound dressing. However, pure BC lacks the antimicrobial activity, which limits its application in wound healing. To solve this problem, copper nanoparticles (Cu NPs) loaded BC membranes were fabricated by using in situ chemical reduction method. The morphology and chemical composition of the composite membranes were characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), Fourier Transform Infrared (FTIR) and thermogravimetric analysis (TGA)...
October 3, 2018: Journal of Biomaterials Science. Polymer Edition
Eun Byul Cho, Gil Soon Park, Seok Soon Park, Ye Ji Jang, Kwang Ho Kim, Kwang Joong Kim, Eun Joo Park
BACKGROUND: Platelet-rich plasma (PRP) is a blood fraction that contains high concentrations of several growth factors. PRP has been recently used in skin wound healing and rejuvenation. However, the precise molecular mechanisms underlying PRP-induced wound healing are unknown. AIMS: This study aimed to evaluate the effects of PRP on extracellular matrix remodeling, which requires the activation of dermal fibroblasts. METHODS: Cell proliferation and migration assay, enzyme-linked immunosorbent analysis, and Western blotting were performed on PRP-treated human skin fibroblasts...
October 2, 2018: Journal of Cosmetic Dermatology
Amanpreet Kaur, Brett L Ecker, Stephen M Douglass, Curtis H Kugel, Marie R Webster, Filipe V Almeida, Rajasekharan Somasundaram, James Hayden, Ehsan Ban, Hossein Ahmadzadeh, Janusz Franco-Barraza, Neelima Shah, Ian A Mellis, Frederick Keeney, Andrew Kossenkov, Hsin-Yao Tang, Xiangfan Yin, Qin Liu, Xiaowei Xu, Mitchell Fane, Patricia Brafford, Meenhard Herlyn, David W Speicher, Jennifer A Wargo, Michael T Tetzlaff, Lauren E Haydu, Arjun Raj, Vivek Shenoy, Edna Cukierman, Ashani T Weeraratna
Physical changes in skin are among the most visible signs of aging. We found that young dermal fibroblasts secrete high levels of extracellular matrix (ECM) constituents, including proteoglycans, glycoproteins and cartilage-linking proteins. The most abundantly secreted was HAPLN1, a hyaluronic and proteoglycan link protein. HAPLN1 was lost in aged fibroblasts, resulting in a more aligned ECM that promoted metastasis of melanoma cells. Reconstituting HAPLN1 inhibited metastasis in an aged microenvironment, in 3D skin reconstruction models, and in vivo...
October 2, 2018: Cancer Discovery
Martin Engel, Rachelle Balez, Sonia Sanz Muñoz, Mauricio Castro Cabral-da-Silva, Claire Helen Stevens, Monique Bax, Dzung Do-Ha, Kuldip Sidhu, Perminder Sachdev, Lezanne Ooi
Peripheral dermal fibroblasts (DF) from a healthy 56 year old female were obtained from the Centre for Healthy Brain Ageing (CHeBA) Biobank, University of New South Wales, under the material transfer agreement with the University of Wollongong. DFs were reprogrammed via mRNA-delivered transcription factors into induced pluripotent stem cells (iPSCs). The generated iPSCs were confirmed to be pluripotent, capable of three germ layer differentiation and are thus a useful resource for creating iPSC-derived healthy human cells of any lineage...
September 19, 2018: Stem Cell Research
Li Wang, Li Xiao, Ruzhi Zhang, Linzi Qiu, Rong Zhang, Haixia Shi
Acrylate/acrylamide copolymers haveexcellent optical properties and biocompatibility and are ideal biomaterials that have been widely used in tissue engineering. Muse cells are a specific subset ofmesenchymal stem cells (MSCs)that havean excellent potential for use in regenerative medicine 
 Objective: This study was designed to investigate the effects of acrylate/acrylamide copolymers on the adhesion, proliferation and pluripotent-like properties of Muse cells, which were derived from normal human dermal fibroblasts (NHDFs) by long-term trypsin incubation (LTT)...
October 2, 2018: Biomedical Materials
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