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Dermal fibroblast

Seong-Wook Seo, Seul-Ki Park, Soo-Jin Oh, Ok Sarah Shin
Exposure to ultraviolet (UV) radiation is a major contributing factor to premature aging (photoaging) and skin cancer. In vitro models of cellular senescence have proven to be very useful for the study of slow and progressive accumulation of damage resulting in the growth arrest of aging skin cells. In this study, we compared UVA-induced cellular responses in non-senescent (NS) vs. senescent (S) human dermal fibroblasts (HDFs). HDFs were irradiated with a single dose of UVA (7.5 J/cm2) and QuantSeq 3' mRNA sequencing was performed to assess differential gene expression...
2018: PloS One
David H Jang, Shawn Owiredu, Abhay Ranganathan, David M Eckmann
While barotrauma, decompression sickness and drowning-related injuries are common morbidities associated with diving and decompression from depth, it remains unclear what impact rapid decompression has on mitochondrial function. In vitro diving simulation was performed with human dermal fibroblast cells subjected to control, air, nitrogen and oxygen dive conditions. With the exception of gas mixture, all other related variables including absolute pressure exposure, dive and decompression rates, and temperature were held constant...
August 15, 2018: American Journal of Physiology. Cell Physiology
Won Il Choi, Youngmin Hwang, Abhishek Sahu, Kiyoon Min, Daekyung Sung, Giyoong Tae, Jeong Ho Chang
The use of injectable materials as a biofiller for soft tissue augmentation has been increasing worldwide. Levan is a biocompatible and inexpensive polysaccharide with great potential in biomaterial applications, but it has not been extensively studied. In this study, we evaluated the potential of levan as a new material for dermal fillers and prepared an injectable and physical levan-based hydrogel by combining levan with Pluronic and carboxymethyl cellulose (CMC). A sol state was prepared by mixing the polymers in a specific ratio at 4 °C for 2 days and the hydrogel was formed by increasing the temperature to 37 °C...
August 15, 2018: Biomaterials Science
Ken Shirato, Tomoko Koda, Jun Takanari, Junetsu Ogasawara, Takuya Sakurai, Hideki Ohno, Takako Kizaki
We recently reported that ETAS 50, a standardized extract from the Asparagus officinalis stem, exerted anti-inflammatory effects on ultraviolet-B- (UV-B-) irradiated normal human dermal fibroblasts (NHDFs) by inhibiting nuclear factor- κ B p65 nuclear import and the resulting interleukin-1 β (IL-1 β ) expression. To further elucidate the antiphotoaging potency of ETAS 50, we examined the anti-inflammatory effects on UV-B-irradiated NHDFs by focusing on the stress-activated mitogen-activated protein kinase (MAPK) and Akt signaling pathways...
2018: Evidence-based Complementary and Alternative Medicine: ECAM
Ariella Zehender, Jingang Huang, Andrea-Hermina Györfi, Alexandru-Emil Matei, Thuong Trinh-Minh, Xiaohan Xu, Yi-Nan Li, Chih-Wei Chen, Jianping Lin, Clara Dees, Christian Beyer, Kolja Gelse, Zhong-Yin Zhang, Christina Bergmann, Andreas Ramming, Walter Birchmeier, Oliver Distler, Georg Schett, Jörg H W Distler
Uncontrolled activation of TGFβ signaling is a common denominator of fibrotic tissue remodeling. Here we characterize the tyrosine phosphatase SHP2 as a molecular checkpoint for TGFβ-induced JAK2/STAT3 signaling and as a potential target for the treatment of fibrosis. TGFβ stimulates the phosphatase activity of SHP2, although this effect is in part counterbalanced by inhibitory effects on SHP2 expression. Stimulation with TGFβ promotes recruitment of SHP2 to JAK2 in fibroblasts with subsequent dephosphorylation of JAK2 at Y570 and activation of STAT3...
August 14, 2018: Nature Communications
Yena Kim, Narae Park, Yeri Alice Rim, Yoojun Nam, Hyerin Jung, Kijun Lee, Ji Hyeon Ju
BACKGROUND: Skin is an organ that plays an important role as a physical barrier and has many other complex functions. Skin mimetics may be useful for studying the pathophysiology of diseases in vitro and for repairing lesions in vivo. Cord blood mononuclear cells (CBMCs) have emerged as a potential cell source for regenerative medicine. Human induced pluripotent stem cells (iPSCs) derived from CBMCs have great potential for allogenic regenerative medicine. Further study is needed on skin differentiation using CBMC-iPSCs...
August 13, 2018: Stem Cell Research & Therapy
Tae-Rin Kwon, Chang Taek Oh, Dong-Ho Bak, Jong Hwan Kim, Joon Seok, Jong Hoon Lee, Su Hwan Lim, Kwang Ho Yoo, Beom Joon Kim, Heesu Kim
ETHNOPHARMACOLOGICAL RELEVANCE: Stichopus japonicus (sea cucumber), edible traditional food in Asia, and its extracts are renowned for their wound healing, pain relieving, and cosmetic effects in traditional medicine. Holothurins, toxins isolated from sea cucumber, are thought to be active components for their beneficial effects. However, researchers have yet to outline specific mechanisms thereof. AIM OF THE STUDY: The present study was designed to evaluate the anti-melanogenic and anti-wrinkle properties of S...
August 10, 2018: Journal of Ethnopharmacology
Paola Spitalieri, Rosa V Talarico, Michela Murdocca, Luana Fontana, Marzia Marcaurelio, Elena Campione, Roberto Massa, Giovanni Meola, Annalucia Serafino, Giuseppe Novelli, Federica Sangiuolo, Annalisa Botta
Human induced pluripotent stem cells (hiPSCs)-patient specific are an innovative tool to reproduce a model of disease in vitro and summarize the pathological phenotype and the disease etiopathology. Myotonic dystrophy type 2 (DM2) is caused by an unstable (CCTG)n expansion in intron 1 of the CNBP gene, leading to a progressive multisystemic disease with muscle, heart and central nervous dysfunctions. The pathogenesis of CNS involvement in DM2 is poorly understood since no cellular or animal models fully recapitulate the molecular and clinical neurodegenerative phenotype of patients...
2018: Frontiers in Physiology
Charles E Ananian, Yadwinder S Dhillon, Carl C Van Gils, D Craig Lindsey, Raymond J Otto, Cyaandi R Dove, Jessica T Pierce, Molly C Saunders
Randomized controlled clinical trials (RCTs), the gold standard to determine treatment efficacy against control, have demonstrated advantages of skin substitutes for the treatment of chronic diabetic foot ulcers (DFUs) in comparison to standard of care (SOC). However, RCTs comparing efficacy between 2 or more skin substitutes are very limited. With growing numbers of new skin substitutes, such studies are essential for treatment and policy-making decisions by wound care providers and payers. In this study, we analyzed clinical outcomes and product cost between a viable cryopreserved placental membrane (vCPM) and a human fibroblast-derived dermal substitute (hFDS) for the treatment of chronic DFUs in a prospective, multicenter, single-blind study...
August 11, 2018: Wound Repair and Regeneration
Richard J Bodnar, Tianbing Yang, Lora H Rigatti, Fang Liu, Alexander Evdokiou, Sandeep Kathju, Latha Satish
BACKGROUND: Pericytes have been shown to have mesenchymal stromal cell-like properties and play a role in tissue regeneration. The goal of this study was to determine whether the addition of a pericyte sheet to a full-thickness dermal wound would enhance the healing of an acute wound. METHODS: Human muscle-derived pericytes and human dermal fibroblasts were formed into cell sheets, then applied to full-thickness excisional wounds on the dorsum of nu/nu mice. Histology was performed to evaluate epidermal and dermal reformation, inflammation and fibrosis...
August 6, 2018: Cytotherapy
Barbara Bellei, Emilia Migliano, Marinella Tedesco, Silvia Caputo, Federica Papaccio, Gianluca Lopez, Mauro Picardo
BACKGROUND: Adipose tissue-derived stem cells are considered to be a promising source in the field of cell therapy and regenerative medicine. In addition to direct cell replacement using adipose tissue or purified stem cells, intercellular molecule exchange by the adipose tissue complex, a vast array of bioactive secretory factors, demonstrated beneficial effects by reducing tissue damage and stimulation of endogenous repair. However, for therapeutic purposes, the use of secretome derivatives, such as full conditioned media or purified exosomes generated in vitro, may present considerable disadvantages for cell manufacturing, storage, product safety, and their potential as a ready-to-go therapeutic product...
August 9, 2018: Stem Cell Research & Therapy
Nilay Duman, Reşat Duman, Murat Tosun, Murat Akıcı, Engin Göksel, Barış Gökçe, Oğuzhan Alagöz
PURPOSE: Folic acid is an essential vitamin participating in DNA synthesis and repair. Recently folic acid has been shown to stimulate DNA-repair capacity in dermal fibroblasts in response to injury. Thus, the present study aimed to investigate the effects of topical folinic acid, a 5-formyl derivative of tetrahydrofolic acid, on wound healing using rat wound model. MATERIALS AND METHODS: A rat wound model was established, and the wound healing was evaluated by macroscopic and histological analyses among vehicle control, 2...
August 6, 2018: Advances in Medical Sciences
Xiao Zhang, Dan Zhang, Shang-Chih Chen, Tina Lamey, Jennifer A Thompson, Terri McLaren, John N De Roach, Fred K Chen, Samuel McLenachan
The human iPSC line LEIi006-A was generated from dermal fibroblasts from a patient with retinitis pigmentosa using episomal plasmids containing OCT4, SOX2, KLF4, L-MYC, LIN28, mir302/367 microRNA and shRNA for p53. The iPSC cells carry compound heterozygous mutations (c.1892A > G and c.2548G > A) in the CRB1 gene. LEIi006-A expressed pluripotent stem cell markers, had a normal karyotype and could be differentiated into endoderm, mesoderm and ectodermal lineages, as well as retinal organoids.
August 2, 2018: Stem Cell Research
Farshad Homayouni Moghadam, Mehrnaz Mesbah-Ardakani, Mohammad Hossein Nasr-Esfahani
Objectives: Based on data from Chinese and Indian traditional herbal medicines, gum resin of Ferula assa-foetida (sometimes referred to asafetida or asafoetida) has several therapeutic applications. The authors of various studies have claimed that asafetida has cytotoxic, antiulcer, anti-neoplasm, anti-cancer, and anti-oxidative effects. In present study, the anti-aging effect of asafetida on senescent human dermal fibroblasts was evaluated. Methods: Senescence was induced in in vitro cultured human dermal fibroblasts (HDFs) through exposure to H2 O2 , and the incidence of senescence was recognized by using cytochemical staining for the activity of β -galactosidase...
September 2017: Journal of Pharmacopuncture
Takashi Yamashita, Katja Lakota, Takashi Taniguchi, Ayumi Yoshizaki, Shinichi Sato, Wen Hong, Xingchun Zhou, Snezn Sodin-Semrl, Feng Fang, Yoshihide Asano, John Varga
The hallmarks of systemic sclerosis (SSc) are autoimmunity, microangiopathy and fibrosis. Skin fibrosis is accompanied by attrition of the dermal white adipose tissue layer, and alterations in the levels and function of adiponectin. Since these findings potentially implicate adiponectin in the pathogenesis of SSc, we employed a novel pharmacological approach to augment adiponectin signaling using AdipoRon, an orally active adiponectin receptor agonist. Chronic treatment with AdipoRon significantly ameliorated bleomycin-induced dermal fibrosis in mice...
August 7, 2018: Scientific Reports
Rebecca M Harman, Megan K He, Sheng Zhang, Gerlinde R VAN DE Walle
BACKGROUND: Impaired cutaneous wound healing is common in humans, and treatments are often ineffective. Based on the significant emotional and economic burden of impaired wound healing, innovative therapies are needed. The potential of mesenchymal stromal cell (MSC)-secreted factors to treat cutaneous wounds is an active area of research that is in need of refinement before effective clinical trials can be initiated. The aims of the present study were to (i) study which MSC-secreted factors stimulate dermal fibroblast (DF) migration in vitro and (ii) evaluate the potential of these factors to promote wound healing in vivo...
August 4, 2018: Cytotherapy
Jing Liu, Yale Liu, Lingling Peng, Juxue Li, Kunyi Wu, Linlin Xia, Jiawen Wu, Sijia Wang, Xuening Wang, Qilu Liu, Weihui Zeng, Yumin Xia
Tumor necrosis factor-like weak inducer of apoptosis (TWEAK) acts by engaging with fibroblast growth factor-inducible 14 (Fn14) to regulate inflammatory responses, fibrosis, and tissue remodeling, which are central in the repair processes of wounds. This study aims to explore the potential role of the TWEAK/Fn14 pathway in the healing of cutaneous burn wounds. Third-degree burns were introduced in the wild-type and Fn14-deficient BALB/c mice, followed by evaluation of wound areas and histological changes. The downstream cytokines including growth factors were also examined in lesional skin...
August 3, 2018: Journal of Investigative Dermatology
Tram T Vuong, Sissel B Rønning, Tamer A E Ahmed, Kristiane Brathagen, Vibeke Høst, Maxwell T Hincke, Henri-Pierre Suso, Mona E Pedersen
Avian eggshell membrane (ESM) is a natural biomaterial that has been used as an alternative natural bandage to cure wounds, and is available in large quantities from egg industries. We have previously demonstrated that processed eggshell membrane powder (PEP), aiming to be used in a low cost wound healing product, possesses anti-inflammatory properties. In this study, we further investigated effects of PEP on MMP activities in vitro (a dermal fibroblast cell culture system) and in vivo (a mouse skin wound healing model)...
2018: PloS One
Haifei Shi, Tingting Weng, Chunmao Han, Xingang Wang
In human adults, the repair of cutaneous wounds usually leads to scar formation rather than regeneration. Dermal substitutes have been used as a regenerative template for reducing scar formation and improving the extent of dermal regeneration. However, achievement of complete regeneration is still a long way off. Dermal substitutes are characterized by unusual regenerative activity, appearing to function by acting as temporary configurational guides for cell infiltration and synthesis of new stroma. Fibroblasts are important cells with many vital functions in wound-healing processes...
August 6, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Armita M Gorabi, Saeideh Hajighasemi, Hossein A Tafti, Amir Atashi, Masoud Soleimani, Nasser Aghdami, Ali K Saeid, Vahid Khori, Yunes Panahi, Amirhossein Sahebkar
BACKGROUND: The discovery of gene- and cell-based strategies has opened a new area to investigate novel approaches for the treatment of many conditions caused by cardiac cell failure. The TBX18 (T-box 18) transcription factor is considered as a prominent factor in the sinoatrial node (SAN) formation during the embryonic development. In this in vitro study, the effect of TBX18 gene expression on human-induced pluripotent-stem-cell-derived cardiomyocytes (hiPS-CMs) to induce pacemaker-like cells was examined...
August 5, 2018: Journal of Cellular Physiology
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