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Collagen degradation

Johannes C Prihadi, Suwandi Sugandi, Nuryati C Siregar, Gunanti Soejono, Alida Harahap
Background: Extracellular matrix degradation may play an important role in the etiology of urethral stricture. MMP1 and TIMP1 are involved in extracellular matrix degradation. The aim of this study was to investigate the roles of MMP1, TIMP1, and MMP1:TIMP1 ratio at the remodeling phase of urethral stricture in an animal model. Methods: This research was carried out in collaboration between the Bogor Institute of Agriculture, Universitas Indonesia, and the Eijkman Institute Indonesia...
2018: Research and Reports in Urology
Menglin Fan, Yanan Li, Chunhua Yao, Xiufeng Liu, Jihua Liu, Boyang Yu
Artemisinins have been reported to have diverse functions, such as antimalaria, anticancer, anti-inflammation, and immunoregulation activities. DC32 [(9α,12α-dihydroartemisinyl) bis(2'-chlorocinnmate)], a dihydroartemisinin derivative possessing potent immunosuppressive properties, was synthesized in our previous study. Collagen-induced arthritis (CIA) in DBA/1 mice and inflammatory model in NIH-3T3 cells were established to evaluate the effect of DC32 on RA and discover the underlying mechanisms. The results showed that DC32 could markedly alleviate footpad inflammation, reduce cartilage degradation, activate the Nrf2/HO-1 signaling pathway, and increase the transcription of p62 in DBA/1 mice with CIA...
2018: Frontiers in Immunology
Ernesto Cortes, Dariusz Lachowski, Benjamin Robinson, Muge Sarper, Jaakko S Teppo, Stephen D Thorpe, Tyler J Lieberthal, Kazunari Iwamoto, David A Lee, Mariko Okada-Hatakeyama, Markku T Varjosalo, Armando E Del Río Hernández
The tumor microenvironment is fundamental to cancer progression, and the influence of its mechanical properties is increasingly being appreciated. Tamoxifen has been used for many years to treat estrogen-positive breast cancer. Here we report that tamoxifen regulates the level and activity of collagen cross-linking and degradative enzymes, and hence the organization of the extracellular matrix, via a mechanism involving both the G protein-coupled estrogen receptor (GPER) and hypoxia-inducible factor-1 alpha (HIF-1A)...
December 11, 2018: EMBO Reports
Mohamadhasan Ansarizadeh, Shohreh Mashayekhan, Maryam Saadatmand
In this study, lyophilized advanced platelet rich fibrin (A-PRF) was used in combination with collagen-chitosan membrane for the first time to combine advantages of both collagen and A-PRF membranes. Response surface methodology (RSM) was used to design the experimental condition and to correlate the effects of parameters, including chitosan/collagen (chit/col) weight ratio and A-PRF concentration on Young's modulus, mesenchymal stem cell (MSCs) viability and degradation rate of the membranes. Results showed that Young's modulus of the membranes was intensified by increasing chit/col weight ratio and decreasing A-PRF concentration from 3 to 8 MPa...
December 8, 2018: International Journal of Biological Macromolecules
Jie Li, Xinyi Kong, Shanshan Jiang, Wenjian Liao, Zhihui Zhang, Junfu Song, Ying Liang, Wei Zhang
Pulmonary fibrosis (PF) is a fibroproliferative disease that can eventually lead to fatal lung failure. It is characterized by abnormal proliferation of fibroblasts, dysregulated fibroblast differentiation to myofibroblast, and disorganized collagen and extracellular matrix production, deposition and degradation. There is still a lack of effective treatment strategies for PF. Extracellular high-mobility group box protein 1 (HMGB1) induces PF through NF-κB-mediated TGF-β1 release. Herein, we first validate the suppressive effect of HMGB1 knockdown on TGF-β1-induced α-smooth muscle actin (α-SMA) and collagen I protein expression...
December 9, 2018: Journal of Cellular Biochemistry
Hao Nie, Hong-Xia Wang, Cui Tian, Hua-Liang Ren, Fang-Da Li, Chao-Yu Wang, Hui-Hua Li, Yue-Hong Zheng
Inflammation plays a critical role in the development of abdominal aortic aneurysm (AAA). Chemokine receptor CXCR2 mediates inflammatory cell chemotaxis in several diseases. However, the role of CXCR2 in AAA and the underlying mechanisms remain unknown. In this study, we found that the CXCR2 expressions in AAA tissues from human and angiotensin II (Ang II)-infused apolipoprotein E knockout (Apo E-/- ) mice were significantly increased. The pharmacological inhibition of CXCR2 (SB265610) markedly reduced Ang II-induced AAA formation...
December 7, 2018: Heart and Vessels
Daniel Guldager Kring Rasmussen, Per Mose Nielsen, Özlem Yashar Kasab-Oglo, Signe Holm Nielsen, Casper Kierulf-Lassen, Morten Asser Karsdal, Federica Genovese, Rikke Nørregaard
Background: Maintenance of kidney function in kidney allografts remains a challenge, as the allograft often progressively develops fibrosis after kidney transplantation. Fibrosis is caused by the accumulation of extracellular matrix proteins like type I and III collagen (COL I and III) that replace the functional tissue. We assessed the concentrations of a neo-epitope fragment of COL III generated by matrix metalloproteinase-9 cleavage (C3M) in two rat models resembling the ischaemic injury taking place following kidney transplantation...
December 7, 2018: Nephrology, Dialysis, Transplantation
Zhong-Nan Hu, Li-Juan Huang, Wei-Ping Chen
The effects of rosmarinic acid (RosA) on osteoarthritis (OA) was investigated in rat chondrocytes. Chondrocytes were isolated from rat cartilage, incubated with RosA in the presence of interleukin-1beta (IL-1β) (10 ng/ml). The production of IL-6, as well as the mRNA level of aggrecan (ACAN) and collagen 2 (COL2), were assessed. The gene and protein expression of A disintegrin and metalloproteinase with thrombospondin motifs (ADAMTS)-4, ADAMTS-5 were also measured. RosA inhibited the production of IL-6 as well as the gene and protein expression of ADAMTS-4 and ADAMTS-5, in addition, RosA abolished IL-1β-induced inhibition of ACAN and COL2 gene expression...
December 7, 2018: Acta Biochimica Polonica
Caiwei Wang, Li Yan, Bo Yan, Li Zhou, Wan Sun, Lingying Yu, Fucun Liu, Wenxi Du, Guangping Yu, Zhengyan Hu, Qiang Yuan, Luwei Xiao, Hongwen Li, Peijian Tong, Jida Zhang, Letian Shan, Thomas Efferth
ETHNOPHARMACOLOGICAL RELEVANCE: Osteoarthritis (OA), characterized by joint pain and cartilage degradation, is the most common form of joint disease worldwide but with no satisfactory therapy available. The ethanol extract of Agkistrodon acutus (EAA) has been widely used as a traditional Chinese medicine (TCM) for the treatment of arthralgia and inflammatory diseases, but there is no report regarding its efficacy on OA to date. Here, we determined the effects of EAA on the pain behavior and cartilage degradation in vivo and clarified its target genes and proteins associated with chondrocyte hypertrophy and apoptosis in vitro...
December 5, 2018: Journal of Ethnopharmacology
Congcong Qu, Zixian Bao, Xin Zhang, Zhiguo Wang, Jizhen Ren, Zhongzheng Zhou, Meiping Tian, Xiaojie Cheng, Xiguang Chen, Chao Feng
Cell therapy with bone marrow-derived mesenchymal stem cells (BMSCs) is a potential method for many disease treatments, including keloid. In the present study, an Arg-Gly-Asp (RGD) modified hydroxybutyl chitosan (HBC) hydrogel (HBC-RGD) was developed to enhance the adhesion and proliferation of BMSCs within the hydrogel. The successful synthesis of HBC-RGD was confirmed by FTIR and 1 H NMR. Both HBC and HBC-RGD hydrogel had desired thermosensitivity, biocompatibility and enzymatic degradability in vitro. Compared with HBC hydrogel, HBC-RGD hydrogel was more beneficial for the adhesion and proliferation of BMSCs...
December 5, 2018: International Journal of Biological Macromolecules
David G Guzzardi, Guoqi Teng, Sean Kang, Patrick J Geeraert, Simranjit S Pattar, Daniyil A Svystonyuk, Darrell D Belke, Paul W M Fedak
OBJECTIVES: Fluoroquinolone (FQ) antibiotics are associated with adverse aortic clinical events. We assessed human aortic myofibroblast-mediated extracellular matrix (ECM) dysregulation as a possible cellular mechanism underlying FQ-associated aortopathy. METHODS: Human aortic myofibroblasts were isolated from patients with aortopathy undergoing elective ascending aortic resection (N = 9). The capacity for extracellular matrix degradation in cells exposed to FQ was assessed by multiplex analysis of secreted matrix metalloproteinases relative to tissue inhibitors of matrix metalloproteinases (TIMPs)...
September 21, 2018: Journal of Thoracic and Cardiovascular Surgery
U Daood, J P Matinlinna, A S Fawzy
OBJECTIVE: Effect of d-alpha-tocopheryl poly(ethyleneglycol)-1000-succinate (VE-TPGS) with riboflavin-5'-phosphate solution on crosslinking of dentine collagen was investigated to analyze collagen's structural integrity. METHODS: VE-TPGS was added to RF-solution, at RF/VE-TPGS (w/w) ratios of 0.125/0.250 and 0.125/0.500. Demineralized dentine beams were used (10wt.% phosphoric acid), rinsed using deionized-water and analysed using ELISA (Human MMP2 ELISA; Human CTSK/Cathepsin-K for MMP2 and Cathepsin K analysis)...
December 7, 2018: Dental Materials: Official Publication of the Academy of Dental Materials
E S Karaarslan, Z C Özmen, F Aytac, A A Bicakci, M Buldur, L Aydogan, F Hologlu, Bbc Özkocak
The color of the teeth is an important topic for many people and can be influenced by intrinsic and extrinsic stains. There is an increasing demand for whitening of the teeth year by year. The most popular way of whitening is "bleaching," which is the result of the breakdown of pigments located in the enamel and/or the dentin, caused by reactive oxygen species (ROS) released from bleaching agents. These bleaching agents could increase matrix metalloproteinase (MMP)-mediated collagen degradation in dentin...
December 9, 2018: Human & Experimental Toxicology
Doretta Cuffaro, Elisa Nuti, Valentina Gifford, Noriko Ito, Caterina Camodeca, Tiziano Tuccinardi, Susanna Nencetti, Elisabetta Orlandini, Yoshifumi Itoh, Armando Rossello
Collagen degradation and proMMP-2 activation are major functions of MT1-MMP to promote cancer cell invasion. Since both processes require MT1-MMP homodimerization on the cell surface, herein we propose that the use of bifunctional inhibitors of this enzyme could represent an innovative approach to efficiently reduce tumor growth. A small series of symmetrical dimers derived from previously described monomeric arylsulfonamide hydroxamates was synthesized and tested in vitro on isolated MMPs. A nanomolar MT1-MMP inhibitor, compound 6, was identified and then submitted to cell-based assays on HT1080 fibrosarcoma cells...
November 29, 2018: Bioorganic & Medicinal Chemistry
Xianxiang Xiang, Yantao Zhou, Honglin Sun, Shiping Tan, Zhanbin Lu, Lixin Huang, Weiming Wang
Ivabradine is most commonly used for the treatment of worsening cardiac failure in patients who cannot tolerate the maximum dose of β-blockers or in whom treatment with β-blockers is contraindicated. While ivabradine is regarded as a highly selective "funny current" (If ) inhibitor, the molecular mechanism behind the effect of this drug remains poorly understood. In the present study, we applied ivabradine in the context of osteoarthritis by treating primary human chondrocytes with tumor necrosis factor-α (TNF-α) and measuring degradation of the articular cartilage matrix as well as the expression of various enzymes and pro-inflammatory cytokines...
December 3, 2018: International Immunopharmacology
Michele Maglione, Lisa Vaccari, Lucia Mancini, Regina Ciancio, Diana Eva Bedolla, Lorenzo Bevilacqua, Paola Tonellato
PURPOSE: The aim of this work was to prove the synergic complementarity of attenuated total reflection Fourier transform infrared microspectroscopy (micro-ATR FTIR), scanning electron microscopy (SEM) coupled with energy-dispersive x-ray spectroscopy (EDS) and x-ray computed microtomography (micro-CT) by studying implant samples with bone affected by peri-implantitis. MATERIALS AND METHODS: Six samples of implanted bone affected by peri-implantitis and one control healthy bone were analyzed...
December 5, 2018: International Journal of Oral & Maxillofacial Implants
Yingting Yu, Qiaojun Situ, Wangyue Jia, Junxiang Li, Qiong Wu, Jinzhi Lei
Coculture of mesenchymal stem cells (MSCs) and vascular endothelial cells (ECs) in vitro leads to the formation of a capillary-like reticular structure by ECs, which has great potential as a better substitute for artificial blood vessels in terms of stability and functionality. To investigate the mechanisms of the early neovascularization induced by MSCs, we analyzed the kinematic features of the motion of ECs and concluded that the dynamic interaction between cells and the extracellular matrix would reveal the capillary-like structure formation...
December 5, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
S K Ranamukhaarachchi, R N Modi, A Han, D O Velez, A Kumar, A J Engler, S I Fraley
Collagen I is the primary extracellular matrix component of most solid tumors and influences metastatic progression. Collagen matrix engineering techniques are useful for understanding how this complex biomaterial regulates cancer cell behavior and for improving in vitro cancer models. Here, we establish an approach to tune collagen fibril architecture using PEG as an inert molecular crowding agent during gelation and cell embedding. We find that crowding produces matrices with tighter fibril networks that are less susceptible to proteinase mediated degradation, but does not significantly alter matrix stiffness...
December 5, 2018: Biomaterials Science
Mingyu He, Luyao Sun, Xiaoling Fu, Sean P McDonough, Chih-Chang Chu
The objective of this study is to design a new family of biodegradable synthetic polymeric biomaterials for providing a tunable inhibition of macrophage's nitric oxide synthase (NOS) pathway. L-Arginine (Arg) is the common substrate for NOS and arginase. Both two metabolic pathways participate in the wound healing process. An impaired wound healing, such as diabetic or other chronic wounds is usually associated with an overproduction of NO by macrophages via the NOS pathway. In this study, a new family of L-nitroarginine (NOArg) based polyester amide (NOArg-PEA) and NOArg-Arg PEA copolymers (co-PEA) were designed and synthesized with different composition ratios...
November 30, 2018: Acta Biomaterialia
Pernille Juhl, Anne-Christine Bay-Jensen, Morten Karsdal, Anne Sofie Siebuhr, Nathalie Franchimont, Juan Chavez
BACKGROUND: Systemic sclerosis (SSc) is characterized by excessive fibrosis throughout the body. This leads to the release of extracellular matrix (ECM) fragments into circulation, where they may be quantified as biomarkers. The objectives were to investigate levels of ECM turnover biomarkers and the diagnostic power of these. METHODS: Diffuse SSc patients (n = 40) fulfilling the ACR/EULAR 2013 classification criteria and asymptomatic controls were included. Patients were divided into early (<2 years of symptoms; n = 20) and late (>10 years of symptoms; n = 20) diffuse SSc...
2018: PloS One
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