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Collagen degradation

Xiaobin Guo, Liangliang Wang, Menglei Xu, Jiaxiang Bai, Jining Shen, Binqing Yu, Yu Liu, Houyi Sun, Yuefeng Hao, Dechun Geng
Abnormal reduction of extracellular matrix (ECM), including type II collagen and aggrecan, caused by tumor necrosis factor-α (TNF-α) is an important pathological feature of osteoarthritis (OA). Shikimic acid (SA), derived from natural plants, has displayed effective pharmacological properties in diverse diseases. The biological roles of SA in OA have not been reported before. Here, we found that treatment with SA (1 mM, 10 mM) prevented TNF-α-induced degradation of type II collagen and aggrecan ECM in human primary chondrocytes culture in vitro...
August 6, 2018: International Immunopharmacology
Xintian Shao, Qixin Chen, Xixi Dou, Lei Chen, Jixu Wu, Wei Zhang, Huarong Shao, Peixue Ling, Fei Liu, Fengshan Wang
We have previously reported an application of lower range of molecular weight of xanthan gum (LRWXG) for inhibiting cartilage matrix destruction and preventing mitochondrial damage in rabbit osteoarthritis (OA) model. However, whether LRWXG exerts its anti-OA activity through intrinsic bax-mitochondria cytochrome c-caspase signaling pathway in OA still requires further study. To address this problem, the OA model was induced by anterior cruciate ligament transection (ACLT) in rabbit and then treated with LRWXG...
October 15, 2018: Carbohydrate Polymers
Fernanda Rossi Paolillo, Vicente Silva Mattos, André Orlandi de Oliveira, Francisco Eduardo Gontijo Guimarães, Vanderlei Salvador Bagnato, Jarbas Caiado de Castro Neto
Diabetes is a complex metabolic disease and has chronic complications. It has been considered a serious public health problem. The aim of the current study was to evaluate skin glycated proteins through fluorescence and Raman techniques. One hundred subjects were invited to participate in the study. Six volunteers did not attend due to exclusion criteria or a change of mind about participating. Therefore, ninety four volunteers were grouped according to age range (20 to 80 years), health condition (non-diabetic, with insulin resistance (IR) and/or diabetic) and Fitzpatrick skin type (I to VI)...
August 9, 2018: Journal of Biophotonics
Xiaoming Li, H Michael Shepard, Jessica A Cowell, Chunmei Zhao, Ryan J Osgood, Sanna Rosengren, Barbara Blouw, Sheryl A Garrovillo, Mark D Pagel, Clifford J Whatcott, Haiyong Han, Daniel D Von Hoff, Darin M Taverna, Michael J Labarre, Daniel C Maneval, Curtis B Thompson
PURPOSE: The tumor microenvironment (TME) evolves to support tumor progression. One marker of more aggressive malignancy is hyaluronan (HA) accumulation. Here we characterize biological and physical changes associated with HA accumulating (HA-high) tumors. EXPERIMENTAL DESIGN: We used immunohistochemistry, in vivo imaging of tumor pH, and microdialysis to characterize the TME of HA-high tumors, including tumor interstitial pressure (tIP), vascular structure, hypoxia, tumor perfusion by doxorubicin, pH, content of collagen and smooth muscle actin (α-SMA)...
July 3, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Barbara Cvikl, Adrian Lussi, Thiago Saads Carvalho, Andreas Moritz, Reinhard Gruber
OBJECTIVES: Matrix metalloproteinases (MMPs) in dentin and saliva can degrade collagen. Divalent metals are known inhibitors of MMPs, but stannous - such as in the form of stannous chloride (SnCl2 ) or stannous fluoride (SnF2 ) - is yet to be tested for a possible inhibitory effect. In this study, we tested the inhibitory effect on the proteolytic activity of MMP-2 and MMP-9. METHODS: Sodium chloride (NaCl), sodium fluoride (NaF), and chlorhexidine (CHX) were used as controls...
August 3, 2018: Journal of Dentistry
Prashant K Bhagwat, Komal K Bhise, Madhuri V Bhuimbar, Padma B Dandge
In this study, novel and cheap sources like fish scales and molasses were used for the production of collagenolytic protease. Statistical optimization of different parameters for the production of collagenolytic protease by Bacillus cereus strain SUK has been carried out using response surface methodology (RSM). Three most significant medium components identified by Plackett-Burman (PB) were fish scales, molasses, and incubation time, which were further optimized using central composite design (CCD). The medium having fish scales 9...
August 3, 2018: Environmental Science and Pollution Research International
Sang-Heon Park, Jinseol Rhee, Seul-Ki Kim, Jung-Ah Kang, Ji-Sun Kwak, Young-Ok Son, Wan-Su Choi, Sung-Gyoo Park, Jang-Soo Chun
BACKGROUND: We recently demonstrated that BATF, a member of the activator protein-1 (AP-1) family, regulates osteoarthritic cartilage destruction. Here, we explored the roles and regulatory mechanisms of BATF in collagen-induced arthritis (CIA) in mice. METHODS: CIA and K/BxN serum transfer were used to generate inflammatory arthritis models in wild-type (WT) and Batf-/- mice. RA manifestations were determined by examining CIA incidence, clinical score, synovitis, synovial hyperplasia, angiogenesis in inflamed synovium, pannus formation, bone erosion, and cartilage destruction...
August 2, 2018: Arthritis Research & Therapy
S N Kehlet, T Manon-Jensen, S Sun, S Brix, D J Leeming, M A Karsdal, N Willumsen
The matricellular protein SPARC (secreted proteome acidic and rich in cysteine) is known to bind collagens and regulate fibrillogenesis. Cleavage of SPARC at a single peptide bond, increases the affinity for collagens up to 20-fold. To investigate if this specific cleavage has pathological relevance in fibrotic disorders, we developed a competitive ELISA targeting the generated neo-epitope on the released fragment and quantified it in serum from patients with lung cancer, idiopathic pulmonary fibrosis (IPF), chronic obstructive pulmonary disease (COPD) and healthy subjects...
August 1, 2018: Cancer Biology & Therapy
Sandro M Sgrott, Rodrigo D Neves, Armando J D'Acampora, Geraldo J S Bernardes, Luiz Belmonte, Thiago C Martins, Franciane Bobinski, Eduardo Cargnin-Ferreira, Andreza Hoepers, Clarissa M Comim, Daniel F Martins, Anna P Piovezan
Silicone breast implant is associated with complications inherent to the surgical procedure. Prosthesis coating with polyurethane, however, commonly reduces the incidence of such complications. In this paper, the authors evaluated the inflammatory histomorphometric profile and oxidative damage associated to the implant of polyester urethane sheets. Forty-eight Wistar rats were divided into Sham or polyester urethane groups (n = 8/group) and underwent a polyester urethane implant in the dorsal skinfold. Tissue samples were collected on days seven, 30, and 90 after surgery and subjected to histomorphometric analysis and biochemical tests...
July 26, 2018: Anais da Academia Brasileira de Ciências
Alessia Castagnino, Antonio Castro-Castro, Marie Irondelle, Alan Guichard, Catalina Lodillinsky, Laetitia Fuhrmann, Sophie Vacher, Sonia Agüera-González, Anna Zagryazhskaya-Masson, Maryse Romao, Carole El Kesrouani, Angelika A Noegel, Thierry Dubois, Graça Raposo, James E Bear, Christoph S Clemen, Anne Vincent-Salomon, Ivan Bièche, Philippe Chavrier
Membrane type 1-matrix metalloproteinase (MT1-MMP), a membrane-tethered protease, is key for matrix breakdown during cancer invasion and metastasis. Assembly of branched actin networks by the Arp2/3 complex is required for MT1-MMP traffic and formation of matrix-degradative invadopodia. Contrasting with the well-established role of actin filament branching factor cortactin in invadopodia function during cancer cell invasion, the contribution of coronin-family debranching factors to invadopodia-based matrix remodeling is not known...
July 31, 2018: Oncogene
Yuki Taga, Osamu Hayashida, Ahmed Ashour, Yhiya Amen, Masashi Kusubata, Kiyoko Ogawa-Goto, Kuniyoshi Shimizu, Shunji Hattori
Hydroxyproline (Hyp) is a collagen-specific amino acid formed by post-translational hydroxylation of Pro residues. Various Hyp-containing oligopeptides are transported into the blood at high concentrations after oral ingestion of collagen hydrolysate. Here we investigated the angiotensin-converting enzyme (ACE) inhibitory activity of X-Hyp-Gly-type tripeptides. In an in vitro assay, ginger-degraded collagen hydrolysate enriched with X-Hyp-Gly-type tripeptides dose-dependently inhibited ACE, and various synthetic X-Hyp-Gly-type tripeptides showed ACE-inhibitory activity...
July 31, 2018: Journal of Agricultural and Food Chemistry
Cecilie F Kjelgaard-Petersen, Neha Sharma, Ashref Kayed, Morten A Karsdal, Ali Mobasheri, Per Hägglund, Anne-Christine Bay-Jensen, Christian S Thudium
OBJECTIVE: Currently, there are no disease-modifying osteoarthritis drugs (DMOADs) approved for osteoarthritis. It is hypothesized that a subtype of OA may be driven by inflammation and may benefit from treatment with anti-inflammatory small molecule inhibitors adopted from treatments of rheumatoid arthritis. This study aimed to investigate how small molecule inhibitors of intracellular signaling modulate cartilage degradation and formation as a pre-clinical model for structural effects...
July 27, 2018: Biochemical Pharmacology
Heinz P Janke, Nihan Güvener, Weiqiang Dou, Dorien M Tiemessen, Anglita YantiSetiasti, Jozef G O Cremers, Paul J A Borm, Wout F J Feitz, Arend Heerschap, Fabian Kiessling, Egbert Oosterwijk
In vivo monitoring of tissue-engineered constructs is important to assess their integrity, remodeling, and degradation. However, this is challenging when the contrast with neighboring tissues is low, necessitating labeling with contrast agents (CAs), but current CAs have limitations (i.e., toxicity, negative contrast, label instability, and/or inappropriate size). Therefore, a naturally derived hemin-L-lysine (HL) complex is used as a potential CA to label collagen-based templates for magnetic resonance imaging (MRI)...
July 29, 2018: Advanced Healthcare Materials
Wanyu Jin, Gaoyang Guo, Liang Chen, Yang Lei, Yunbing Wang
The lifetime of bioprosthetic heart valves (BHVs) is limited by the mechanical damage and calcification. The major components of BHVs are collagen and elastin. Collagen could be well protected by glutaraldehyde (GLUT) crosslinking, while elastin is not stabilized and has a high risk of degradation, which could lead to the calcification of BHVs. We aimed to develop methods for stabilizing elastin and decreasing calcification. We investigated the combined tannic acid (TA) or epigallocatechin gallate (EGCG) with ferric chloride to stabilize elastin and prevent calcification...
July 29, 2018: Artificial Organs
A C Arismendi Sosa, A G Salinas Ibáñez, M V Pérez Chaca, A B Penissi, N N Gómez, A E Vega
Helicobacter pylori infection has been reported to be associated with extra-digestive disorders such as respiratory diseases; however, the impact of H. pylori on lung is incompletely understood. Inflammatory response is mediated by the release of cytokines, interferon, and enzymes such as metalloproteinases (MMPs). This may contribute to collagen accumulation during the early phase of infection. MMP expression is an important factor for the proliferation and infiltration of lung cells in the process of fibrosis formation...
July 26, 2018: Microbial Pathogenesis
Cai-Xia Li, Li-Hua Cui, Yu-Zhen Zhuo, Jian-Gong Hu, Nai-Qiang Cui, Shu-Kun Zhang
AIMS: Autophagy is an intracellular metabolic process that degrades and recycles own constituents to maintain homeostasis and supply substrates. Disruption of collagen degradation is one of the pathogenesis of pancreatic fibrosis. In this study, we investigated the effects of inhibiting autophagy on the collagen degradation of PSCs. MAIN METHODS: Rats were injected dibutyltin dichloride (DBTC) to induce chronic pancreatitis (CP) model. The expression of LC3B was measured by western blotting...
July 26, 2018: Life Sciences
Shunsuke Uehara, Nobuyuki Udagawa, Yasuhiro Kobayashi
Osteoclasts are multinucleated cells responsible for bone resorption. Osteoclasts adhere to the bone surface through integrins and polarize to form actin rings, which are formed by the assembly of podosomes. The area contained within actin rings (also called sealing zones) has an acidic pH, which causes dissolution of bone minerals including hydroxyapatite and the degradation of matrix proteins including type I collagen by the protease cathepsin K. Osteoclasts resorb bone matrices while moving on bone surfaces...
July 26, 2018: Cellular and Molecular Life Sciences: CMLS
Harjit Pal Bhattoa
With an aging population, there is a marked increase in prevalence of metabolic bone diseases, especially osteoporosis. Perhaps the most dreaded complication of metabolic bone disease, fractures typically impose a huge burden on the ailing body and are associated with high co-morbidity and mortality. The consequent public health and socioeconomic burden warrant timely diagnosis, treatment and follow-up of these disorders. Knowing the limitations of radiological techniques, biochemical markers of bone turnover measurements come handy since the changes in their levels readily reflect bone physiology...
July 2018: EJIFCC
Si-Yang Yu, Bo Dong, Zhen-Fei Fang, Xin-Qun Hu, Liang Tang, Sheng-Hua Zhou
This study was aimed at investigating the effects of lncRNA AK139328 on myocardial ischaemia/reperfusion injury (MIRI) in diabetic mice. Ischaemia/reperfusion (I/R) model was constructed in normal mice (NM) and diabetic mice (DM). Microarray analysis was utilized to identify lncRNA AK139328 overexpressed in DM after myocardial ischaemia/reperfusion (MI/R). RT-qPCR assay was utilized to investigate the expressions of lncRNA AK139328 and miR-204-3p in cardiomyocyte and tissues. Left ventricular end diastolic diameter (LVEDD), left ventricular end systolic diameter (LVESD), left ventricular ejection fraction (LVEF) and fractioning shortening (FS) were obtained by transthoracic echocardiography...
July 25, 2018: Journal of Cellular and Molecular Medicine
Guo Wei, Qingqing Xu, Lin Liu, Huanhuan Zhang, Xi Tan, Chunhong Zhang, Changyu Han, Yanxia Guo, Ganwen Han, Chunmin Zhang
Hypertrophic scars (HS) are fibro-hyperproliferative dermal lesions with effusive continuous accumulation of extracellular matrix components, particularly collagen. They usually occur after dermal injury in genetically susceptible individuals and cause both physical and psychological distress for the affected individuals. Transforming growth factor-β1 (TGF-β1) is known to mediate wound healing process by regulating cell differentiation, collagen production and extracellular matrix degradation. The sustained high expression of TGF-β1 is believed to result in the formation of hypertrophic scars...
July 25, 2018: Archives of Dermatological Research
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