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Collagen degradation

Flavia Squeglia, Alessia Ruggiero, Rita Berisio
The scenario of chemical reactions prompted by the infection by Mycobacterium tuberculosis is huge. The infection generates a localized inflammatory response, with the recruitment of neutrophils, monocytes, and T-lymphocytes. Consequences of this immune reaction can be the eradication or containment of the infection, but these events can be deleterious to the host inasmuch as lung tissue can be destroyed. Indeed, a hallmark of tuberculosis (TB) is the formation of lung cavities, which increase disease development and transmission, as they are sites of high mycobacterial burden...
October 11, 2018: Biochemical Journal
Yue Yang, Yang Wang, Yawei Kong, Xiaoning Zhang, He Zhang, Yi Gang, Lunhao Bai
Mechanical stress plays a key role in regulating cartilage degradation in osteoarthritis (OA). The aim of this study was to evaluate the effects and mechanisms of mechanical stress on articular cartilage. A total of 80 male Sprague-Dawley rats were randomly divided into eight groups (n = 10 for each group): control group (CG), OA group (OAG), and CG or OAG subjected to low-, moderate-, or high-intensity treadmill exercise (CL, CM, CH, OAL, OAM, and OAH, respectively). Chondrocytes were obtained from the knee joints of rats; they were cultured on Bioflex 6-well culture plates and subjected to different durations of cyclic tensile strain (CTS) with or without exposure to interleukin-1β (IL-1β)...
October 12, 2018: Journal of Cellular Physiology
Elahe Hadavi, Jeroen C H Leijten, Jenny Brinkmann, Pascal Jonkheijm, Marcel Karperien, Aart Alexander van Apeldoorn
Extracellular matrix molecules play significant roles in regulating β-cell function and viability within pancreatic islets by providing mechanical and biological support, stimulating cell survival, proliferation and their endocrine function. During clinical islet transplantation the beta cell's extracellular matrix environment is degraded by enzymatic digestion. Literature suggests that interactions between islet cells and ECM molecules, such as fibronectin, collagen IV and laminin, are essential for maintaining, or stimulation of islet function and survival, and can effect differentiation and proliferation of the endocrine cells...
October 11, 2018: Tissue Engineering. Part C, Methods
Lu-Yao Wang, Wang-Sheng Wang, Ya-Wei Wang, Jiang-Wen Lu, Yi Lu, Chu-Yue Zhang, Wen-Jiao Li, Kang Sun, Hao Ying
Preterm premature rupture of fetal membranes precedes 30-40% of preterm births. Activation of matrix metalloproteases (MMPs) is the one of the major causes of extracellular matrix (ECM) degradation in membrane rupture. Increased cortisol, regenerated by 11β-hydroxysteroid dehydrogenase 1 in the amnion at parturition, is known to participate in a number of parturition-pertinent events. However, whether cortisol has a role in the regulation of MMPs in the membranes is not known. Here, we addressed this issue using human amnion tissue, the most tensile layer of the membranes...
October 10, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Jianying Zhang, Daibang Nie, Jorge L Rocha, MaCalus V Hogan, James H-C Wang
In this study, we report that human plantar fascia consists of two distinct tissues with differential structural properties. These tissues also contain stem/progenitor cells with differential biological properties. The mechanobiological responses of these two plantar fascia stem cells also differ in terms of expression of collagen I and IV, non-ligament-related genes, and proinflammatory genes. The production of inflammatory agents (prostaglandin E2 , interleukin-6) and matrix degradative enzymes (matrix metalloproteinase-1, matrix metalloproteinase-2) are also different between the two types of plantar fascia stem cells...
January 2018: Journal of Tissue Engineering
Cameron A Stewart, Yoav Finer
OBJECTIVES: Despite decades of development and their status as the restorative material of choice for dentists, resin composite restoratives and adhesives exhibit a number of shortcomings that limit their long-term survival in the oral cavity. Herein we review past and current work to understand these challenges and approaches to improve dental materials and extend restoration service life. METHODS: Peer-reviewed work from a number of researchers as well as our own are summarized and analyzed...
October 6, 2018: Dental Materials: Official Publication of the Academy of Dental Materials
Xijiao Yu, Hongmei Liu, Shuang Liu, Xue Chen, Xinyu Zhao, Yi Du, Shu Li
BACKGROUND AND OBJECTIVE: Periodontal ligament-associated protein-1 (PLAP-1) is an important regulator of osteogenic differentiation of periodontal ligament cells and plays important role in the homeostasis of periodontal tissues. But the role of PLAP-1 in periodontitis is poorly understood. Expressions of PLAP-1 in experimental periodontitis are observed to elucidate whether PLAP-1 gets involved in the pathogenesis of periodontitis. MATERIAL AND METHODS: Wistar rats were randomly allocated to two groups (n = 6/group): Ligation group and Control group...
October 8, 2018: Journal of Periodontal Research
Zahra Bazrafshan, George K Stylios
One step fabrication of the three dimension (3D) fibrous structure of Collagen-g-poly(MMA-co-EA)/Nylon6 was investigated by controlling the experimental conditions during coaxial electrospinning. This 3D fibrous structure is the result of interactions of two polymeric systems with a varied capability to be electrostatically polarized under the influence of the external electric field; the solution with the higher conductivity into the inner spinneret and the solution with the lesser conductivity into the outer capillary of the coaxial needle...
October 8, 2018: Nanomaterials
Laure Magnan, Gaëlle Labrunie, Sylvie Rey, Nathalie Dusserre, Nicolas L'Heureux, Sébastien Marais, Sabrina Lacomme, Etienne Gontier, Marc Bonneu
We have previously shown that the Cell-Assembled extracellular Matrix (CAM) synthesized by normal, human, skin fibroblasts in vitro can be assembled in a completely biological vascular graft that was successfully tested in the clinic. The goal of this study was to perform a detailed analysis of the composition and the organization of this truly bio-material. In addition, we investigated whether the devitalization process (dehydration) used to store the CAM, and thus, make the material available "off-the-shelf," could negatively affect its organization and mechanical properties...
October 5, 2018: Acta Biomaterialia
Kristi J Warren, Jill A Poole, Jenea M Sweeter, Jane M DeVasure, Todd A Wyatt
Matrix metalloproteinases are important for proper airway matrix structure and wound healing. These enzymes are also implicated in many airway diseases. Previously, chronic ethanol consumption was shown to prolong inflammation and delay viral clearance in respiratory syncytial virus (RSV)-infected mice. We hypothesize that alcohol alters anti-viral immunity by disrupting immune cell chemotaxis in the lung. BALB/c mice were randomly selected to consume 18% alcohol ad libitum for 8 weeks prior to infection with RSV-2A...
October 3, 2018: Alcohol
Lindsey Nicol, Patrick Morar, Ying Wang, Kim Henriksen, Shu Sun, Morten Karsdal, Rosamund Smith, Sandesh C S Nagamani, Jay Shapiro, Brendan Lee, Eric Orwoll
Osteogenesis imperfecta [1] is a rare disorder of connective tissue caused by abnormalities in the synthesis or processing of type I collagen. Type I collagen is the most abundant type of collagen and is expressed in almost all connective tissues. Given that type I collagen interacts with other collagens based in the extracellular matrix (ECM), we hypothesized changes in type I collagen in OI would result in perturbations in the homeostasis of other collagen types. We measured serum biomarkers of several non-type I collagens in patients with mild (type I) and moderate-to-severe (type III/IV) OI...
October 2, 2018: Bone
Shakib Omari, Elena Makareeva, Anna Roberts-Pilgrim, Lynn Mirigian, Michal Jarnik, Carolyn Ott, Jennifer Lippincott-Schwartz, Sergey Leikin
Type I collagen is the main component of bone matrix and other connective tissues. Rerouting of its procollagen precursor to a degradative pathway is crucial for osteoblast survival in pathologies involving excessive intracellular buildup of procollagen that is improperly folded and/or trafficked. What cellular mechanisms underlie this rerouting remains unclear. To study these mechanisms, we employed live-cell imaging and correlative light and electron microscopy (CLEM) to examine procollagen trafficking both in wild-type mouse osteoblasts and osteoblasts expressing a bone pathology-causing mutant procollagen...
October 4, 2018: Proceedings of the National Academy of Sciences of the United States of America
Zixian Bao, Yue Sun, Kamal Rai, Xinying Peng, Shilu Wang, Rui Nian, Mo Xian
Collagen has been widely documented as one of the most promising and competitive biomaterials for tissue engineering and medical applications. However, the properties of collagen differ from one source to another. In the present study, type I collagen (COL-I) was extracted and purified from the skins of Japanese sea bass (Lateolabrax japonicus) and Nile tilapia (Oreochromis niloticus). Ultraviolet (UV) spectroscopy, Fourier transform infrared spectroscopy (FTIR) and SDS-PAGE were performed to characterize both COL-Is...
October 4, 2018: Biomaterials Science
Huiyong Liu, Honglei Kang, Chao Song, Zuowei Lei, Li Li, Jianfeng Guo, Yong Xu, Hanfeng Guan, Zhong Fang, Feng Li
Low back pain (LBP) is a common worldwide disease that causes an enormous social economic burden. Intervertebral disc degeneration (IDD) is considered as a major cause of LBP. The process of IDD is complicated and involves both inflammation and senescence. The production of pro-inflammatory cytokines, including tumor necrosis factor (TNF)α and interleukin (IL)-1β, is increased in the degenerating intervertebral disc, inducing extracellular matrix degradation. Urolithin A (UA) is a metabolite compound resulting from the degradation of ellagitannins by gut bacteria...
2018: Frontiers in Pharmacology
H-X Zhang, C Sun, H-C Yu, B Song, Z-X Pan
OBJECTIVE: Wnt/β-catenin pathway plays a critical role in modulating embryonic development, cell growth, and differentiation. The over-expression of β-catenin activates this pathway and up-regulates expression of matrix metalloproteinase-13 (MMP-13), and promotes matrix degradation and occurrence of osteoarthritis (OA). This study aims to explore the effect of miR-320 expression in OA chondrocyte and underlying mechanisms. PATIENTS AND METHODS: Chondrocyte tissues from OA patients and normal individuals were collected for the detection of expression levels of miR-320, β-catenin, MMP-13, and alpha-1 chain of type II collagen (COL2A1)...
September 2018: European Review for Medical and Pharmacological Sciences
Salvatore Sauro, Vicente Faus-Matoses, Irina Makeeva, Juan Manuel Nuñez Martí, Raquel Gonzalez Martínez, José Antonio García Bautista, Vicente Faus-Llácer
OBJECTIVES: Resin-modified glass ionomer cements (RMGIC) are considered excellent restorative materials with unique therapeutic and anti-cariogenic activity. However, concerns exist regarding the use of polyacrylic acid as a dentine conditioner as it may influence the bonding performance of RMGIC. The aim of this study was to evaluate the effect of different protocols for cycling mechanical stress on the bond durability and interfacial ultramorphology of a modern RMGIC applied to dentine pre-treated with/without polyacrylic acid conditioner (PAA)...
October 2, 2018: Materials
Karan Arora, Mackenzie Herroon, Malik H Al-Afyouni, Nicholas P Toupin, Thomas N Rohrabaugh, Lauren M Loftus, Izabela Podgorski, Claudia Turro, Jeremy J Kodanko
Dual action agents containing a cysteine protease inhibitor and Ru-based photosensitizer for photodynamic therapy (PDT) were designed, synthesized and validated in 2D culture and 3D functional imaging assays of triple-negative human breast cancer (TNBC). These combination agents deliver and release Ru-based PDT agents to tumor cells and cause cancer cell death upon irradiation with visible light, while at the same time inactivating cathespin B (CTSB), a cysteine protease strongly associated with invasive and metastatic behavior...
October 2, 2018: Journal of the American Chemical Society
Kyunghee Lee, Incheol Seo, Mun Hwan Choi, Daewon Jeong
Bone undergoes continuous remodeling, which is homeostatically regulated by concerted communication between bone-forming osteoblasts and bone-degrading osteoclasts. Multinucleated giant osteoclasts are the only specialized cells that degrade or resorb the organic and inorganic bone components. They secrete proteases (e.g., cathepsin K) that degrade the organic collagenous matrix and establish localized acidosis at the bone-resorbing site through proton-pumping to facilitate the dissolution of inorganic mineral...
October 1, 2018: International Journal of Molecular Sciences
Giriprasath Ramanathan, Sitalakshmi Thyagarajan, Uma Tiruchirapalli Sivagnanam
The prolonged inflammation and elevation of Matrix Metalloproteniases (MMPs) at the wound site causes significant degradation of Extracellular matrix (ECM) which cause delays the process of wound healing. Hence the development of therapeutic dressing matrices to control and to positively regulate MMPs balance was considered important in achieving faster healing. The design of biomaterial matrices of collagen scaffold has the challenge to mimic the function of ECM and emulate to the attraction of fibroblast migration at wound site...
December 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
S Rößler, C Heinemann, B Kruppke, A S Wagner, S Wenisch, H P Wiesmann, T Hanke
The intent of the present study was to demonstrate that multiphasic silica/collagen xerogels are able to manipulate cellular processes. These xerogels were prepared by a sol-gel approach allowing the incorporation of mineral phases. The resulting nanocomposites are designed as biomaterial for bone regeneration. Human osteoclasts derived from peripheral blood mononuclear cells were cultured both indirectly and directly, either in presence of different xerogel types or on their surface, to investigate the factor with the main influence on osteoclastogenesis...
December 1, 2018: Materials Science & Engineering. C, Materials for Biological Applications
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