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circRNA or "circular RNA"

Yuping Zhou, Xueyou Lv, Hui Qu, Kekai Zhao, Liyun Fu, Linwen Zhu, Guoliang Ye, Junming Guo
OBJECTIVE: To screen for circular RNAs (circRNAs) that are associated with the activation of hepatic stellate cell (HSC) by monitoring changes in liver circRNA expression in a model of liver fibrosis. METHODS: The classic mouse model of CCl4 -induced liver fibrosis was established and validated by histopathological examination. JS1 cells were activated by TGF-β1 to model HSC activation in vitro. Differentially expressed circRNAs in the fibrotic liver tissues and JS1 cells were determined using circRNA microarray, and some of those circRNAs were verified by RT-qPCR...
August 14, 2018: Gene
Meng-Yuan Zhou, Jin-Ming Yang, Xing-Dong Xiong
Circular RNAs (circRNAs), a large novel type of endogenous transcripts, have become a new research hotspot in the field of RNA biology. CircRNAs are mainly generated from exons or introns via multiple mechanisms, and the majority of circRNAs are stable and conserved across different species. Recent studies have revealed that circRNAs can function as miRNA sponges, binding partners of proteins, regulators of transcription, or can even be translated into proteins. Growing evidence has demonstrated that circRNAs play important roles in a wide variety of biological processes such as cell proliferation, apoptosis and senescence, and may serve as potential diagnostic biomarkers or therapeutic targets for various cardiovascular diseases...
August 14, 2018: Journal of Molecular and Cellular Cardiology
Zhi Long, Juan Xie, Yan-Ping Liu, Li Zhang, Chen-Guang Xu, Jiang-Yan Luo, Guo-Qing Li
The purpose of this study was to investigate the expression profile of circular RNA (circRNA) in gastric cancer cells MGC-803, SGC-7901 and NCI-N87 with different degrees of differentiation and normal gastric epithelial cells GES-1. High throughput circRNA microarray technique was used to detect the differential expression of circRNA between three kinds of differentiated gastric cancer cells and normal gastric epithelial cells. The interaction of microRNAs (miRNAs) with circRNAs was predicted by bioinformatics software, and circRNA, which might have great significance in gastric cancer, was identified by literature search...
August 25, 2018: Sheng Li Xue Bao: [Acta Physiologica Sinica]
Yuan Fang
Circular RNAs (circRNAs) are a large class of noncoding RNAs characterized with closed loop structures without 3' and 5' polar ends. They can roughly be divided into exonic circRNAs, exon-intron circRNAs and circular intronic RNAs. CircRNAs are characterized with stability, prevalence, specificity and conservation, which arouse great interest in circRNAs as disease biomarkers. Their abilities to sponge to miRNAs, cis-regulate parent genes, bind to proteins and encode proteins endow circRNAs a critical role of regulation in eukaryotic cells...
July 2018: Future Science OA
Aimiao Bian, Yanping Wang, Ji Liu, Xiaodong Wang, Dai Liu, Jian Jiang, Lianshu Ding, Xiaobo Hui
BACKGROUND Circular RNAs (circRNAs) are widely expressed in mammals and can regulate the development and progression of human tumors. has_circ_0015758 (circ-CFH) is an exon circRNA transcript from the GRCh37/hg19 fragment of chromosome 1 and is homologous to the protein-coding gene complement factor H (CFH). Currently, the function of circ-CFH in glioma remains unclear. MATERIAL AND METHODS In our study, circ-CFH, miR-149, and Akt1 mRNA expression levels were analyzed by qRT-PCR assays. To investigate the function of circ-CFH in cell proliferation, circ-CFH knockdown models were established by using circ-CFH siRNAs...
August 16, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Xianglun Cui, Jianxun Wang, Zongjun Guo, Mengyang Li, Mingyu Li, Si Liu, Haoran Liu, Wenjing Li, Xunhua Yin, Jiaping Tao, Wenhua Xu
As a novel class of endogenous RNAs, circRNAs, have a covalently closed continuous loop, with neither a 5'to 3'polarity, nor a polyadenylated tail. Numerous circRNAs have been characterized by abundance, stabilization, conservation, and exhibit tissue/developmental stage-specific expression. Furthermore, circRNAs play vital roles in tumorigenesis and metastasis, such as functioning as a ceRNA or miRNA sponge, interacting with protein and encoding protein. Increasing evidence has revealed that it potentially serves as a required novel biomarker for cancer diagnosis...
August 15, 2018: Molecular Cancer
Chong Chen, Haining Tan, Jiaqi Bi, Zheng Li, Tianhua Rong, Youxi Lin, Liang Sun, Xingye Li, Jianxiong Shen
BACKGROUND/AIMS: Congenital scoliosis (CS) is a result of anomalous development of vertebrae and is frequently associated with somitogenesis malformation. Although noncoding RNAs (ncRNAs) have been recently determined to be involved in the pathogenesis of CS, the competing endogenous RNA (ceRNA) regulatory networks in CS remain largely unknown. METHODS: Sequencing was conducted to explore the ncRNA expression profiles in rat embryos (gestation day 9) following vitamin A deficiency (VAD) (n = 9 for the vitamin A deficiency-induced congenital scoliosis (VAD-CS) group and n = 4 for the control group)...
August 15, 2018: Cellular Physiology and Biochemistry
Yuan Gao, Mingli Wu, Yingzhi Fan, Shipeng Li, Zhenyu Lai, Yongzhen Huang, Xianyong Lan, Chuzhao Lei, Hong Chen, Ruihua Dang
Circular RNA (circRNA) is a new class of non-coding RNA that has recently attracted researchers' interest. Studies have demonstrated that circRNA can function as microRNA sponges or competing endogenous RNAs. Although circRNA has been explored in some species and tissues, the genetic basis of testis development and spermatogenesis in cattle remains unknown. We performed ribo-depleted total RNA-Seq to detect circRNA expression profiles of neonatal (one week old) and adult (4 years old) Qinchuan cattle testes...
July 2018: Royal Society Open Science
Yongsheng Li, Jiahang Hu, Lijuan Li, Shengyan Cai, Hengchun Zhang, Xianfeng Zhu, Guijie Guan, Xiangmei Dong
Non-small cell lung cancer (NSCLC) is an aggressive malignancy with increasing worldwide incidence and is characterized by dismal prognosis due to its early recurrence and metastasis. Accumulating evidence documented that aberrantly expressed circular RNAs (circRNAs) are critically involved in tumorigenesis. In the current study, we focused on a novel circRNA, circ_0016760 in NSCLC. qRT-PCR was carried out to determine the expression level of circ_0016760 in tissue samples and cell lines. The clinical value of circ_0016760 was also investigated...
August 6, 2018: Biochemical and Biophysical Research Communications
Xianwen Meng, Peijing Zhang, Qi Chen, Jingjing Wang, Ming Chen
BACKGROUND: Leaf development is a complex biological process that is accompanied by wide transcriptional changes. Many protein-coding genes have been characterized in plant leaves, but little attention has been given to noncoding RNAs (ncRNAs). Moreover, increasing evidence indicates that an intricate interplay among RNA species, including protein-coding RNAs and ncRNAs, exists in eukaryotic transcriptomes, however, it remains elusive in plant leaves. RESULTS: We detected novel ncRNAs, such as circular RNAs (circRNAs) and long noncoding RNAs (lncRNAs), and further constructed and analyzed their associated competitive endogenous RNA (ceRNA) networks in Arabidopsis leaves...
August 13, 2018: BMC Genomics
Junming Bi, Hongwei Liu, Zijian Cai, Wei Dong, Ning Jiang, Meihua Yang, Jian Huang, Tianxin Lin
Circ-BPTF (hsa_circ_0000799) is a novel circular RNA derived from BPTF exons. Although BPTF is a well-studied predecessor gene, the characteristics and functions of circ-BPTF have not yet been reported. Here, we show that expression of circ-BPTF is increased in bladder cancer (BCa) tissues and cell lines compared with noncancerous tissues and cell lines. Consistently, BCa patients with higher expression levels of circ-BPTF were found to have higher tumor grades and poorer prognosis. Functionally, knockdown of circ-BPTF inhibited tumor progression in vitro and in vivo...
August 9, 2018: Aging
Xiaolong Hu, Min Zhu, Bo Liu, Zi Liang, Lixu Huang, Jian Xu, Lei Yu, Kun Li, Mengsheng Jiang, Renyu Xue, Guangli Cao, Chengliang Gong
Thus far, no systematic studies have examined circRNA expression profiles in the silkworm following B.mori nucleopolyhedrovirus (BmNPV) infection. To explore the expression patterns of circRNAs in the silkworm midgut following BmNPV infection, circRNAs in normal midguts and BmNPV-infected midguts were analyzed by high-throughput sequencing. A total of 353 circRNAs were significantly differentially expressed, of which 241 were upregulated and 112 were downregulated following infection. GO annotation and KEGG pathways analyses of these circRNAs showed that many key immunity pathways and metabolism pathways were enriched in the BmNPV-infected midguts...
August 10, 2018: Molecular Immunology
Shuaixi Yang, Zhenqiang Sun, Quanbo Zhou, Weiwei Wang, Guixian Wang, Junmin Song, Zhen Li, Zhiyong Zhang, Yuan Chang, Kunkun Xia, Jinbo Liu, Weitang Yuan
Noncoding RNAs (ncRNAs) can be divided into microRNAs (miRNAs), long noncoding RNAs (lncRNAs), circular RNAs (circRNAs), pRNAs, and tRNAs. Traditionally, miRNAs exert their biological function mainly through the inhibition of translation via the induction of target RNA transcript degradation. lncRNAs and circRNAs were once considered to have no potential to code proteins. Here, we will review the current knowledge on ncRNAs in relation to their origins, characteristics, and functions. We will also review how ncRNAs work as competitive endogenous RNA, gene transcription and expression regulators, and RNA-binding protein sponges in colorectal cancer (CRC)...
2018: Cancer Management and Research
Ning Xu, Shaomu Chen, Yufeng Liu, Wei Li, Zeyi Liu, Xiaojie Bian, Chunhua Ling, Min Jiang
BACKGROUND/AIMS: Circular RNAs (circRNAs) act as microRNA (miRNA) sponges that regulate gene expression and are involved in physiological and pathological processes. In this study, we evaluated the roles of circRNAs in the chemoresistance of non-small cell lung cancer (NSCLC) to taxol. METHODS: High-throughput circRNA microarrays were employed to investigate the circRNA profiles of parental A549 and taxol-resistant A549/Taxol cells. We predicted the miRNA targets of differentially expressed circRNAs via miRNA prediction software and then constructed a circRNA/miRNA network using Cytoscape...
August 10, 2018: Cellular Physiology and Biochemistry
Zhi-Bin Zhou, Yu-Long Niu, Gao-Xiang Huang, Jia-Jia Lu, Aimin Chen, Lei Zhu
Circular RNAs (circRNAs) represent a class of non-coding RNAs that are involved in transcriptional and posttranscriptional gene expression regulation and associated with different kinds of human diseases. However, the characterization and function of circular RNAs in peripheral nerve injuries remain elusive. Here, we established a rat sciatic nerve injury model and identified at least 4,942 distinct circular RNA candidates and a series of circular RNAs that were differentially expressed in injured nerve tissues compared with matched normal tissues...
July 25, 2018: Molecular Therapy. Nucleic Acids
Weiping Huang, Yongyong Lu, Feng Wang, Xixi Huang, Zhixian Yu
Although increasing aberrantly expressed circular RNAs (circRNAs) have been identified among many human cancer tissues, their roles in tumor progression still remain largely unknown. In bladder cancer, the function of hsa_circ_0000144 has not been reported. In our study, we found hsa_circ_0000144 as a novel oncogene in bladder cancer by bioinformatics analysis. We found that hsa_circ_0000144 expression was significantly upregulated in bladder cancer tissues compared with adjacent normal tissues, and its high expression was related with poor prognosis...
August 8, 2018: Gene
Jia-Qi Sheng, Lian Liu, Mu-Ru Wang, Pei-Yuan Li
Circular RNAs (circRNAs) are a series of special closed circular RNA molecules with stability and conservatism. In recent years, advances in high-throughput RNA sequencing technology have led to explosive discovery of circRNAs in different types of species and cells. Moreover, circRNAs can accomplish a remarkable multitude of biological functions, such as regulating transcription or splicing, serving as miRNA sponges, interacting with RNA-binding proteins, and translating proteins. Meanwhile, circRNAs involve in the biogenesis and development of many diseases, including cardiovascular disorders, nervous system disorders, cancers, etc...
2018: American Journal of Cancer Research
Tong Zhou, Xueying Xie, Musheng Li, Junchao Shi, Jin Zhou, Kenneth Knox, Ting Wang, Qi Chen, Wanjun Gu
Circular RNAs (circRNAs) are a novel class of regulatory RNAs. Here, we present a comprehensive investigation of circRNA expression profiles across 11 tissues and 4 developmental stages in rats, along with cross-species analyses in humans and mice. Although the expression of circRNAs is positively correlated with that of cognate mRNAs, highly expressed genes tend to splice a larger fraction of circular transcripts. Moreover, circRNAs exhibit higher tissue specificity than cognate mRNAs. Intriguingly, while we observed a monotonic increase of circRNA abundance with age in the rat brain, we further discovered a dynamic, age-dependent pattern of circRNA expression in the testes that is characterized by a dramatic increase with advancing stages of sexual maturity and a decrease with aging...
August 9, 2018: RNA
Dan-Dan Xiong, Yi-Wu Dang, Peng Lin, Dong-Yue Wen, Rong-Quan He, Dian-Zhong Luo, Zhen-Bo Feng, Gang Chen
BACKGROUND: Circular RNAs (circRNAs) have received increasing attention in human tumor research. However, there are still a large number of unknown circRNAs that need to be deciphered. The aim of this study is to unearth novel circRNAs as well as their action mechanisms in hepatocellular carcinoma (HCC). METHODS: A combinative strategy of big data mining, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and computational biology was employed to dig HCC-related circRNAs and to explore their potential action mechanisms...
August 9, 2018: Journal of Translational Medicine
Jinchang Pan, Xiaodan Meng, Nan Jiang, Xiaofeng Jin, Chengwei Zhou, Dazhi Xu, Zhaohui Gong
Considerable evidence has indicated that most noncoding RNA (ncRNA) transcripts act directly as functional RNAs rather than as encoded peptides. However, a recent study of ribosome occupancy reported that many large intergenic ncRNAs (lincRNAs) are bound by ribosomes, raising the possibility that they are translated into proteins. These lincRNAs contain either internal ribosomal entry sites (IRES) or short open reading frames (sORFs), and other translation elements, which can be translated into peptides with physiological functions...
August 9, 2018: Protein and Peptide Letters
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