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Umbilical Stem Cell Injection

Li-Chun Zhang, Yu Wang, Wei Liu, Xue-Min Zhang, Miao Fan, Min Zhao
AIMS: To study the protective effects and mechanisms of human umbilical cord mesenchymal stem cells (hUCMSCs) and overexpression of antioxidant gene SOD2 on lung injury by establishing a rat model of paraquat (PQ)-induced lung injury. MAIN METHODS: The hUCMSCs cell line overexpressed SOD2 was established. After intraperitoneal injection of PQ solution (24 mg/kg) 3 h later, the different groups of hUCMSCs cell lines were injected through the tail veins of rats...
October 12, 2018: Life Sciences
Insung Kang, Byung-Chul Lee, Jin Young Lee, Jae-Jun Kim, Seung-Eun Lee, Nari Shin, Soon Won Choi, Kyung-Sun Kang
Mesenchymal stem cells (MSCs) are multipotent adult stem cells that present immunosuppressive effects in experimental and clinical trials targeting various rare diseases including inflammatory bowel disease (IBD). In addition, recent studies have reported tryptophanyl-tRNA synthetase (WRS) possess uncanonical roles such as angiostatic and anti-inflammatory effects. However, little is known about the function of WRS in MSC-based therapy. In this study, we investigated if a novel factor, WRS, secreted from MSCs has a role in amelioration of IBD symptoms and determined a specific mechanism underlying MSC therapy...
October 8, 2018: BMB Reports
Dong Rak Kwon, Gi-Young Park, Yong Suk Moon, Sang Chul Lee
While therapies using mesenchymal stem cells (MSCs) to treat rotator cuff tendon tear (RCTT) have yielded some promising preliminary results, MSCs therapy has not yet completely regenerated full-thickness RCTT (FTRCTT). It has recently been reported that polydeoxyribonucleotide (PDRN) is effective in the treatment of chronic rotator cuff disease. We hypothesized that local injection of human umbilical cord blood-derived (UCB)-MSCs with PDRN would be more effective in regenerating tendon tear than UCB-MSCs alone...
October 1, 2018: Cell Transplantation
Mao Ding, Yang Shen, Ping Wang, Zhaohong Xie, Shunliang Xu, ZhengYu Zhu, Yun Wang, Yongtao Lyu, Dewei Wang, Linlin Xu, JianZhong Bi, Hui Yang
Alzheimer's disease (AD) is the most common neurodegenerative disease characterized by excessive accumulation of the amyloid-β peptide (Aβ) in the brain, which has been considered to mediate the neuroinflammation process. Microglial activation is the main component of neuroimmunoregulation. In recent years, exosomes isolated from human umbilical cord mesenchymal stem cells (hucMSC-exosomes) have been demonstrated to mimic the therapeutic effects of hucMSCs in many inflammation-related diseases. In this study, exosomes from the supernatant of hucMSCs were injected into AD mouse models...
September 26, 2018: Neurochemical Research
Junhui Zhang, Bingjun Xiong, Juanjuan Qiu, Qiuwen Tan, Yi Zhang, Jiqiao Yang, Qing Lü
Objective: To investigate the effects of adipose-derived stem cells (ADSCs) and endothelial cells (ECs) on the survival and neovascularization of fat tissue transplants. Methods: The ADSCs were isolated by collagenase digestion from the adipose tissues voluntarily donated by the patients undergoing mastectomy, and subcultured. The passage 3 ADSCs were used for subsequent experiments. The residual fat tissues were used to prepare fat particles (FPs). The human umbilical vein endothelial cells (HUVECs) were used as ECs for subsequent experiments...
August 1, 2018: Chinese Journal of Reparative and Reconstructive Surgery
Francesca Bonifazi, Elisa Dan, Myriam Labopin, Mariarosaria Sessa, Viviana Guadagnuolo, Martina Ferioli, Simonetta Rizzi, Sabrina De Carolis, Barbara Sinigaglia, Maria Rosa Motta, Andrea Bontadini, Valeria Giudice, Giovanni Martinelli, Mario Arpinati, Michele Cavo, Massimiliano Bonafé, Gianluca Storci
Umbilical Cord Blood (UCB) represents a valid option for patients with hematopoietic malignancies lacking an HLA matched donor. To overcome the limitation of the low stem cell dose of UCB, the intrabone (IB) route has been proposed. We report the results of a prospective study on a poor-prognosis cohort of 23 patients receiving intrabone single UCB transplant ( NCT00886522). Cumulative incidence of hematological recovery at day 90 was 82 ± 9% (ANC > 0.5 × 109 /L) and 70 ± 10% (platelet > 50 × 109 /L) and correlated with CD34 + cells in the graft...
September 19, 2018: Bone Marrow Transplantation
Dong Hyun Kim, Dahm Lee, Hoon Lim, Soo Jin Choi, Wonil Oh, Yoon Sun Yang, Jeong Ho Chang, Hong Bae Jeon
Alzheimer's disease (AD), which is the most common progressive neurodegenerative disease, causes learning and memory impairment. The pathological progress of AD can derive from imbalanced homeostasis of amyloid beta (Aβ) in the brain. In such cases, microglia play important roles in regulating the brain Aβ levels. In the present study, we found that human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) can increase, through paracrine action, the ability of microglial cells to clear Aβ. In order to identify the associated paracrine factors, a secretome of hUCB-MSCs co-cultured with Aβ-treated BV2 microglial cells was analyzed using a human cytokine protein array...
October 12, 2018: Biochemical and Biophysical Research Communications
Junyu Fan, Xiaojun Tang, Qian Wang, Zhuoya Zhang, Shufang Wu, Wenchao Li, Shanshan Liu, Genhong Yao, Hongwei Chen, Lingyun Sun
BACKGROUND: Mesenchymal stem cells (MSCs) play an anti-inflammatory role by secreting certain bioactive molecules to exert their therapeutic effects for disease treatment. However, the underlying mechanism of MSCs in chronic autoimmune liver diseases-primary biliary cholangitis (PBC), for example-remains to be elucidated. METHODS: Human umbilical cord-derived MSCs (UC-MSCs) were injected intravenously into 2-octynoic acid coupled to bovine serum albumin (2OA-BSA)-induced autoimmune cholangitis mice...
September 17, 2018: Stem Cell Research & Therapy
So Yoon Ahn, Yun Sil Chang, Young Eun Kim, Se In Sung, Dong Kyung Sung, Won Soon Park
OBJECTIVE: Neonatal meningitis caused by Escherichia coli results in significant mortality and neurological disabilities, with few effective treatments. Recently, we demonstrated that human umbilical cord blood-derived mesenchymal stem cell (hUCB-MSC) transplantation attenuated E. coli-induced severe pneumonia, primarily by reducing inflammation and enhancing bacterial clearance. This study aimed to determine whether intraventricular transplantation of hUCB-MSCs attenuated the brain injury in E...
August 14, 2018: Pediatric Research
Sheng-Jie Shiue, Ruey-Horng Rau, Han-Shiang Shiue, Yi-Wei Hung, Zhi-Xiang Li, Kuender D Yang, Jen-Kun Cheng
Nerve injury-induced neuropathic pain is difficult to treat. In this study, we used exosomes derived from human umbilical cord mesenchymal stem cell (UCMSC) as a cell-free therapy for nerve injury-induced pain in rats. Isolated UCMSC exosomes range in size from 30 to 160 nm and contain CD63, HSP60, and CD81 exosome markers. After L5/6 spinal nerve ligation surgery, single intrathecal injection of exosomes reversed nerve ligation-induced mechanical and thermal hypersensitivities of right hindpaw of rats at initial and well-developed pain stages...
September 3, 2018: Pain
Xiaomin Zhang, Juping Liu, Bo Yu, Feifei Ma, Xinjun Ren, Xiaorong Li
PURPOSE: To assess the efficacy of mesenchymal stem cells (MSCs) and MSC-derived exosomes (MSC-Exos) to promote the healing of large and refractory macular holes (MHs). METHODS: Seven patients (age 51-75 years old) with large and long-standing idiopathic MHs underwent vitrectomy, internal limiting membrane peeling, MSC (two patients) or MSC-Exo (five patients) intravitreal injection, and heavy silicon oil, air, 20% SF6, or 14% C3F8 tamponade. The MSCs were isolated from human umbilical cord tissue, and MSC-Exos were isolated from the supernatants of cultured MSCs using sequential ultracentrifugation...
August 30, 2018: Graefe's Archive for Clinical and Experimental Ophthalmology
Nike Hendrijantini, Tuti Kusumaningsih, Rostiny Rostiny, Pungky Mulawardhana, Coen Pramono Danudiningrat, Fedik Abdul Rantam
Objective: The aim of this study is to prove that human umbilical cord mesenchymal stem cell (hUCMSC) therapy on mandibular osteoporotic model is able to increase transforming growth factor-beta-1 (TGF)-β1 expression, Runx2, and osteoblasts. Materials and Methods: This research is true experimental posttest control group design. Thirty female Wistar rats were divided into 6 groups randomly, which consisted of sham surgery for control (T1), ovariectomy as osteoporotic group (T2), osteoporotic group injected with gelatine for 4 weeks (T3), 8 weeks (T4) injected with hUCMSC-gelatine for 4 weeks (T5) and 8 weeks (T6)...
July 2018: European Journal of Dentistry
Rajasekaran Subbarayan, Dinesh Murugan Girija, Suresh Ranga Rao
Present-day scaffolds are useful in cell therapy to a reasonable extent, but in pursuit of improvising the scaffold to improve the outcome, we tested a new injectable caffeic acid-bioconjugated gelatin hydrogel scaffold (CBGH; with tunable stiffness -10%). Two-dimensional (2D) form of human umbilical cord tissue-derived mesenchymal stem cells (HUCMSCs) culture performed based on our previously reported methods and characterized by using multipotent and pluripotent analysis. In addition, neurogenesis was induced in the presence of retinoic acid or neural growth factor or epidermal growth factor categorized by neuronal markers...
August 24, 2018: Journal of Cellular Physiology
Tatiana Chadid, Andrew Morris, Alexandra Surowiec, Scott Robinson, Maiko Sasaki, Jacques Galipeau, Brian P Pollack, Luke P Brewster
OBJECTIVE: Regenerative medicine seeks to stall or to reverse the pathologic consequences of chronic diseases. Many people with diabetes have peripheral arterial disease (PAD), which increases their already high risk of major amputation. Cellular therapies are a promising regenerative medicine approach to PAD that can be used to focally inject regenerative cells to endangered tissue beds. Mesenchymal stem cells (MSCs) are known to promote tissue regeneration through stromal support and paracrine stimulation of new blood vessels (angiogenesis)...
August 10, 2018: Journal of Vascular Surgery
Caroline Laroye, Jérémie Lemarié, Amir Boufenzer, Pierre Labroca, Lisiane Cunat, Corentine Alauzet, Frédérique Groubatch, Clémence Cailac, Lucie Jolly, Danièle Bensoussan, Loïc Reppel, Sébastien Gibot
BACKGROUND: Septic shock is the leading cause of death in intensive care units. The pathophysiological complexity of this syndrome contributes to an absence of specific treatment. Several preclinical studies in murine models of septic shock have shown improvements to organ injury and survival after administration of mesenchymal stem cells (MSCs). To better mimic a clinical approach in humans, we investigated the impact of randomized controlled double-blind administration of clinical-grade umbilical cord-derived MSCs to a relevant pig model of septic shock...
August 8, 2018: Intensive Care Medicine Experimental
Q Chang, X L Tian, R J Huo, D Liu, J Y Chen, X Wang, X R Tian
Objective: To observe the effect of human umbilical cord mesenchymal stem cells (hUC-MSCs) on airway remodeling in asthma model of rat and its possible mechanism. Methods: hUC-MSCs were isolated and cultured, and surface markers of hUC-MSCs were identified by flow cytometry. Forty Wistar male rats were divided into 4 groups: Control, Model, MSCs, Budesonide. The rats of Control group were sensitized and challenged by normal saline. The rats of Model, MSCs and Budesonide group were sensitized and challenged by ovalbumin (OVA) for 8 weeks...
July 24, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
Marcelo Paulo Vaccari Mazzetti, Nivaldo Alonso, Ryane Schmidt Brock, Alexandre Ayoub, Sally Mizukami Massumoto, Lilian Piñero Eça
Cleft lip and palate is a congenital malformation that requires a multidisciplinary treatment that evolves pediatrician, obstetrics, fetal medicine, genetics, plastic surgery, orthodontics, speech therapist, nursery, and psychology. Actually, the authors believe that it could be possible to ad protocols to use stem cells.The intrauterine diagnosis leads to preborn parental orientation and better parental collaboration to accept a precocious multidisciplinary treatment. After birth the authors' protocol is: orthodontic devices, phonoaudiology, and surgical procedures...
July 31, 2018: Journal of Craniofacial Surgery
De-Bin Guo, Xiang-Qing Zhu, Qing-Qing Li, Gao-Mi-Yang Liu, Guang-Ping Ruan, Rong-Qing Pang, Yu-Hao Chen, Qiang Wang, Jin-Xiang Wang, Ju-Fen Liu, Qiang Chen, Xing-Hua Pan
Umbilical cord mesenchymal stem cells (UC-MSCs) exert strong immunomodulatory effects and can repair organs. However, their roles in radiation injury remain unclear. We show that in tree shrews with acute radiation injury, injected UC-MSCs significantly improved survival rates, reduced lung inflammation and apoptosis, prevented pulmonary fibrotic processes, recovered hematopoiesis, and increased blood counts. A protein microarray analysis showed that serum levels of the anti-inflammatory cytokines IL-10 and IL-13 and the growth factors BMP-5, BMP-7, HGF, insulin, NT-4, VEGFR3, and SCF were significantly higher, while those of the inflammatory cytokines IL-2, TIMP-2, TNF-α, IFN-γ, IL-1ra, and IL-8 and the fibrosis-related factors PDGF-BB, PDGF-AA, TGF-β1, IGFBP-2, and IGFBP-4 were significantly lower in UC-MSC-injected animals...
July 31, 2018: Cytotechnology
Yaoxiang Sun, Hui Shi, Siqi Yin, Cheng Ji, Xu Zhang, Bin Zhang, Peipei Wu, Yinghong Shi, Fei Mao, Yongmin Yan, Wenrong Xu, Hui Qian
Exosomes are nanosized extracellular vesicles (EVs) that show great promise in tissue regeneration and injury repair as mesenchymal stem cell (MSC). MSC has been shown to alleviate diabetes mellitus (DM) in both animal models and clinical trials. In this study, we aimed to investigate whether exosomes from human umbilical cord MSC (hucMSC-ex) have a therapeutic effect on type 2 DM (T2DM). We established a rat model of T2DM using a high-fat diet and streptozotocin (STZ). We found that the intravenous injection of hucMSC-ex reduced blood glucose levels as a main paracrine approach of MSC...
August 28, 2018: ACS Nano
Manal M Kamel, Hanan G El Baz, Zeinab Demerdash, Salwa Hassan, Faten Salah, Wafaa A Mansour, Olfat Hammam, Shimaa Atta, Ali Bayoumi, Marwa Hassan, Soheir Mahmoud
BACKGROUND: The liver is one of the major target organs for which cell-based therapies are very promising. The limitations of various cellular therapies, including bone marrow (BM)-derived mesenchymal stem cells (MSCs), urges the exploration of stem cell sources more suitable for transplantation. Human umbilical cord blood (HUCB) can overcome these drawbacks with a favorable reparative outcome. OBJECTIVES: The aim of this study was to evaluate the therapeutic potential of MSCs in 2 groups of chronic liver injury experimental models...
July 26, 2018: Advances in Clinical and Experimental Medicine: Official Organ Wroclaw Medical University
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