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Umbilical Stem Cell Injection

Negar Varaa, Saeed Azandeh, Layasadat Khorsandi, Darioush Bijan Nejad, Vahid Bayati, Amin Bahreini
Objectives: In this study, effects of encapsulated umbilical cord stem cells (UCSCs)-derived hepatocyte-like cells (HLCs) in high mannuronic alginate scaffolds was investigated on CCl4 -induced acute liver failure (ALF) in rats. Material and Methods: UCSCs were encapsulated in high mannuronic alginate scaffolds. Then the UCSCs differentiated into HLCs for treatment of CCl4 -induced ALF in rats. Thirty rats randomly divided into 5 groups: Intoxicated group received only CCl4 to induce ALF...
September 2018: Iranian Journal of Basic Medical Sciences
Xin Gu, Shi Hua, Matz Ethan, Liren Zhong, Ting Long, Cara Clouse, Wei Li, Dong Chen, HyunChul Chung, Sean Murphy, James Yoo, Guiting Lin, Tom Lu, Anthony Atala, Jackson John, Yuanyuan Zhang
OBJECTIVE: To determine the long-term therapeutic effect among three types of human cell types on erectile function recovery in a rodent model of dual neurovascular injured erectile dysfunction (NVED). MATERIALS AND METHODS: A dual NVED model was established in athymic rats by crushing the bilateral cavernous nerves and ligations of the bilateral internal pudendal nerve-vessel bundles. At the time of defect creation, three different types of human cell populations (2...
November 30, 2018: BJU International
Yahong Chen, Ke Xue, Xiaodie Zhang, Zhiwei Zheng, Kai Liu
BACKGROUND: Developing cartilage constructed with the appropriate matrix composition and persistent chondrogenesis remains an enduring challenge in cartilage defects. Cartilage progenitor cell (CPC)-based tissue engineering has attracted recent attention because of its strong chondrogenic differentiation capacity. However, due to the lack of a suitable chondrogenic niche, the clinical application of CPC-regenerated cartilage in the subcutaneous environment remains a challenge. In this study, exosomes derived from chondrocytes (CC-Exos) were used to provide the CPC constructs with a cartilage signal in subcutaneous environments for efficient ectopic cartilage regeneration...
November 21, 2018: Stem Cell Research & Therapy
Kai Mu, Jing Zhang, Yan Gu, Hongjuan Li, Yan Han, Na Cheng, Xiaoyu Feng, Guoyu Ding, Rongjun Zhang, Yuqi Zhao, Hongmei Wang
RATIONALE: To explore the curative effect of human umbilical cord-derived mesenchymal stem cell (ucMSC) therapy for patients with liver cirrhosis complicated with immune thrombocytopenia and refractory Henoch-Schonlein purpura (HSP). PATIENT CONCERNS: A 12-year-old boy presented to our hospital with an 11-month history of purpura on the skin of both lower limbs accompanied by thrombocytopenia. The patient had a history of repeated swelling and painful dorsum pedis, followed by skin redness...
November 2018: Medicine (Baltimore)
Xiaojie Lv, Yunen Liu, Subin Song, Changci Tong, Xiuyun Shi, Yan Zhao, Jinsong Zhang, Mingxiao Hou
In situ injectable hydrogels for wound healing based on carboxymethyl chitosan (CMCS) and alginate were developed in this work. The liquid mixture of CMCS and alginate solutions formed a gel by polyelectrolyte complexation after addition of d-glucono-δ-lactone (GDL), which slowly hydrolyzed and donated protons. When chitosan oligosaccharide (COS) was added into the mixture, a two-stage gelling process occurred. The primary gelling process was similar to that of the hydrogel without COS, while the secondary gelling process appeared about 20 min later, and much stronger hydrogels with storage modulus G' about 1 MPa, 104 times higher, were obtained...
February 1, 2019: Carbohydrate Polymers
Surya Agus Prihatno, Irma Padeta, Arinda Devi Larasati, Betty Sundari, Annisa Hidayati, Yuda Heru Fibrianto, Teguh Budipitojo
Background: Testicular dysfunction is a degenerative disorder characterized by failure in the synthesis of reproductive hormones and spermatogenesis. Secretome derived from the human umbilical mesenchymal stem cell (MSC) has been reported to repair some degenerative disorders. Aim: This study aimed to investigate the effect of secretome derived from the human umbilical MSCs on cisplatin-induced testicular dysfunction in rats. Materials and Methods: Thirty-six male Wistar rats were divided into the control and secretome-treated groups...
September 2018: Veterinary World
Saisai Huang, Shufang Wu, Zhuoya Zhang, Wei Deng, Junyu Fan, Ruihai Feng, Wei Kong, Jingjing Qi, Weiwei Chen, Xiaojun Tang, Genhong Yao, Xuebing Feng, Dandan Wang, Hongwei Chen, Lingyun Sun
Umbilical cord-derived mesenchymal stem cell transplantation (UCMSCT) has been used to treat human autoimmune diseases like lupus for example, but little is known about its effect on cell apoptosis. Here we evaluated the efficacy of UCMSCT for lupus treatment and explored the mechanism by which mesenchymal stem cells (MSCs) modulate T cell apoptosis in lupus mice. 1 × 106 human umbilical cord-derived mesenchymal stem cells (UC-MSCs) were injected into B6.MRL-Faslpr (B6.lpr) mice via tail vein. 6 h, 24 h or 4 weeks later, the mice were sacrificed and the apoptosis of lymphocytes in peripheral blood and spleen were detected by flow cytometry...
December 9, 2018: Biochemical and Biophysical Research Communications
Ramanathan Yegappan, Vignesh Selvaprithiviraj, Sivashanmugam Amirthalingam, Annapoorna Mohandas, Nathaniel S Hwang, R Jayakumar
Functional biomaterials that couple angiogenesis and osteogenesis processes are vital for bone tissue engineering and bone remodeling. Herein we developed an injectable carrageenan nanocomposite hydrogel incorporated with whitlockite nanoparticles and an angiogenic drug, dimethyloxallylglycine. Synthesized whitlockite nanoparticles and nanocomposite hydrogels were characterized using SEM, TEM, EDS and FTIR. Developed hydrogels were injectable, mechanically stable, cytocompatible and has better protein adsorption...
October 26, 2018: International Journal of Biological Macromolecules
Irina Arutyunyan, Timur Fatkhudinov, Andrey Elchaninov, Olesya Vasyukova, Andrey Makarov, Natalia Usman, Evgeniya Kananykhina, Anastasiya Lokhonina, Dmitry Goldshtein, Galina Bolshakova, Gennady Sukhikh
This study delves into possible mechanisms underlying the stimulating influence of UC-MSCs transplantation on functional and structural recovery of ischemic skeletal muscles. Limb ischemia was created in Sprague-Dawley rats by excision of femoral and popliteal arteries. Allogeneic rat PKH26-labeled UC-MSCs were administered by direct intramuscular injection. Animals of experimental group responded to the transplantation by improvement in their locomotor function as assessed by the rotarod performance test on day 9 and 29 after transplantation...
November 6, 2018: Histology and Histopathology
Hector Lopez Hernandez, Abigail K Grosskopf, Lyndsay M Stapleton, Gillie Agmon, Eric A Appel
Drug delivery and cell transplantation require minimally invasive deployment strategies such as injection through clinically relevant high-gauge needles. Supramolecular hydrogels comprising dodecyl-modified hydroxypropylmethylcellulose and poly(ethylene glycol)-block-poly(lactic acid) have been previously demonstrated for the delivery of drugs and proteins. Here, it is demonstrated that the rheological properties of these hydrogels allow for facile injectability, an increase of cell viability after injection when compared to cell viabilities of cells injected in phosphate-buffered saline, and homogeneous cell suspensions that do not settle...
October 22, 2018: Macromolecular Bioscience
Friederike Philipp, Anton Selich, Michael Rothe, Dirk Hoffmann, Susanne Rittinghausen, Michael A Morgan, Denise Klatt, Silke Glage, Stefan Lienenklaus, Vanessa Neuhaus, Katherina Sewald, Armin Braun, Axel Schambach
Hematopoietic stem cells (HSCs) ensure a life-long regeneration of the blood system and are therefore an important source for transplantation and gene therapy. The teratoma environment supports the complex development of functional HSCs from human pluripotent stem cells, which is difficult to recapitulate in culture. This model mimics various aspects of early hematopoiesis, but is restricted by the low spontaneous hematopoiesis rate. In this study, a feasible protocol for robust hematopoiesis has been elaborated...
November 13, 2018: Stem Cell Reports
C-S Bao, X-L Li, L Liu, B Wang, F-B Yang, L-G Chen
OBJECTIVE: This research aimed to investigate the therapeutic effects of transplanted human umbilical cord mesenchymal stem cells (hUCMSCs) on spinal cord injury in mice and to explore its molecular mechanism. MATERIALS AND METHODS: Spinal cord injury model in C57BL/6J mice was established. On the 10th day of SCI, hUCMSCs were injected into the center of spinal cord injury area (hUCMSC), and control groups (Control) were injected with an equal amount of medium. Western blotting, Real Time-PCR, immunohistochemistry, and flow cytometry, were used to analyze the content of IL-7, inflammatory cytokines, and macrophages after spinal cord injury in different groups...
October 2018: European Review for Medical and Pharmacological Sciences
Li-Chun Zhang, Yu Wang, Wei Liu, Xue-Min Zhang, Miao Fan, Min Zhao
AIMS: To study the protective effects and mechanisms of human umbilical cord mesenchymal stem cells (hUCMSCs) and overexpression of antioxidant gene SOD2 on lung injury by establishing a rat model of paraquat (PQ)-induced lung injury. MAIN METHODS: The hUCMSCs cell line overexpressed SOD2 was established. After intraperitoneal injection of PQ solution (24 mg/kg) 3 h later, the different groups of hUCMSCs cell lines were injected through the tail veins of rats...
December 1, 2018: Life Sciences
Insung Kang, Byung-Chul Lee, Jin Young Lee, Jae-Jun Kim, Seung-Eun Lee, Nari Shin, Soon Won Choi, Kyung-Sun Kang
Mesenchymal stem cells (MSCs) are multipotent adult stem cells that present immunosuppressive effects in experimental and clinical trials targeting various rare diseases including inflammatory bowel disease (IBD). In addition, recent studies have reported tryptophanyl-tRNA synthetase (WRS) possess uncanonical roles such as angiostatic and anti-inflammatory effects. However, little is known about the function of WRS in MSC-based therapy. In this study, we investigated if a novel factor, WRS, secreted from MSCs has a role in amelioration of IBD symptoms and determined a specific mechanism underlying MSC therapy...
October 8, 2018: BMB Reports
Dong Rak Kwon, Gi-Young Park, Yong Suk Moon, Sang Chul Lee
While therapies using mesenchymal stem cells (MSCs) to treat rotator cuff tendon tear (RCTT) have yielded some promising preliminary results, MSCs therapy has not yet completely regenerated full-thickness RCTT (FTRCTT). It has recently been reported that polydeoxyribonucleotide (PDRN) is effective in the treatment of chronic rotator cuff disease. We hypothesized that local injection of human umbilical cord blood-derived (UCB)-MSCs with PDRN would be more effective in regenerating tendon tear than UCB-MSCs alone...
October 1, 2018: Cell Transplantation
Mao Ding, Yang Shen, Ping Wang, Zhaohong Xie, Shunliang Xu, ZhengYu Zhu, Yun Wang, Yongtao Lyu, Dewei Wang, Linlin Xu, JianZhong Bi, Hui Yang
Alzheimer's disease (AD) is the most common neurodegenerative disease characterized by excessive accumulation of the amyloid-β peptide (Aβ) in the brain, which has been considered to mediate the neuroinflammation process. Microglial activation is the main component of neuroimmunoregulation. In recent years, exosomes isolated from human umbilical cord mesenchymal stem cells (hucMSC-exosomes) have been demonstrated to mimic the therapeutic effects of hucMSCs in many inflammation-related diseases. In this study, exosomes from the supernatant of hucMSCs were injected into AD mouse models...
November 2018: Neurochemical Research
Junhui Zhang, Bingjun Xiong, Juanjuan Qiu, Qiuwen Tan, Yi Zhang, Jiqiao Yang, Qing Lü
Objective: To investigate the effects of adipose-derived stem cells (ADSCs) and endothelial cells (ECs) on the survival and neovascularization of fat tissue transplants. Methods: The ADSCs were isolated by collagenase digestion from the adipose tissues voluntarily donated by the patients undergoing mastectomy, and subcultured. The passage 3 ADSCs were used for subsequent experiments. The residual fat tissues were used to prepare fat particles (FPs). The human umbilical vein endothelial cells (HUVECs) were used as ECs for subsequent experiments...
August 1, 2018: Chinese Journal of Reparative and Reconstructive Surgery
Francesca Bonifazi, Elisa Dan, Myriam Labopin, Mariarosaria Sessa, Viviana Guadagnuolo, Martina Ferioli, Simonetta Rizzi, Sabrina De Carolis, Barbara Sinigaglia, Maria Rosa Motta, Andrea Bontadini, Valeria Giudice, Giovanni Martinelli, Mario Arpinati, Michele Cavo, Massimiliano Bonafé, Gianluca Storci
Umbilical Cord Blood (UCB) represents a valid option for patients with hematopoietic malignancies lacking an HLA matched donor. To overcome the limitation of the low stem cell dose of UCB, the intrabone (IB) route has been proposed. We report the results of a prospective study on a poor-prognosis cohort of 23 patients receiving intrabone single UCB transplant ( NCT00886522). Cumulative incidence of hematological recovery at day 90 was 82 ± 9% (ANC > 0.5 × 109 /L) and 70 ± 10% (platelet > 50 × 109 /L) and correlated with CD34 + cells in the graft...
September 19, 2018: Bone Marrow Transplantation
Dong Hyun Kim, Dahm Lee, Hoon Lim, Soo Jin Choi, Wonil Oh, Yoon Sun Yang, Jeong Ho Chang, Hong Bae Jeon
Alzheimer's disease (AD), which is the most common progressive neurodegenerative disease, causes learning and memory impairment. The pathological progress of AD can derive from imbalanced homeostasis of amyloid beta (Aβ) in the brain. In such cases, microglia play important roles in regulating the brain Aβ levels. In the present study, we found that human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) can increase, through paracrine action, the ability of microglial cells to clear Aβ. In order to identify the associated paracrine factors, a secretome of hUCB-MSCs co-cultured with Aβ-treated BV2 microglial cells was analyzed using a human cytokine protein array...
October 12, 2018: Biochemical and Biophysical Research Communications
Junyu Fan, Xiaojun Tang, Qian Wang, Zhuoya Zhang, Shufang Wu, Wenchao Li, Shanshan Liu, Genhong Yao, Hongwei Chen, Lingyun Sun
BACKGROUND: Mesenchymal stem cells (MSCs) play an anti-inflammatory role by secreting certain bioactive molecules to exert their therapeutic effects for disease treatment. However, the underlying mechanism of MSCs in chronic autoimmune liver diseases-primary biliary cholangitis (PBC), for example-remains to be elucidated. METHODS: Human umbilical cord-derived MSCs (UC-MSCs) were injected intravenously into 2-octynoic acid coupled to bovine serum albumin (2OA-BSA)-induced autoimmune cholangitis mice...
September 17, 2018: Stem Cell Research & Therapy
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