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Marisha Sharma, Sunil K Gupta, Baisakhi Majumder, Vivek K Maurya, Farah Deeba, Afroz Alam, Vivek Pandey
Drought is a major concern for sustainable yield under changing environment. Soybean, an economically important oil and protein crop, is prone to drought resulting in yield instability. Salicylic acid (SA), a multifaceted growth hormone, modulates a series of parallel processes to confer drought tolerance thereby relieving yield limitations. The present study was performed in soybean plants treated with SA (0.5 mM) through seed pretreatment under drought regimes: severe stress (50% RWC) and moderate stress (75% RWC), and rehydration...
August 6, 2018: Plant Physiology and Biochemistry: PPB
Shaoping Huang, Senyang Cao, Tao Zhou, Lu Kong, Geyu Liang
4-tert-octylphenol (4t-OP) is a well-known xenoestrogen. Our objective was to explore the effects and molecular mechanisms of 4t-OP on human sperm. Sperm samples were exposed to 0, 0.1, or 0.3 mM 4t-OP for two hours. Results showed that both sperm viability and motility were significantly injured by 0.3 mM 4t-OP. We applied comparative proteomics to explore the molecular targets affected by 4t-OP. 81 differentially expressed (DE) proteins were identified. Bioinformatic analysis showed that these proteins were highly associated with motility and apoptosis, and were mostly enriched in cAMP-PKA/PKC-phosphorylation-associated pathway...
August 3, 2018: Environmental Toxicology and Pharmacology
Tielei Tang, Yonggang Cheng, Qing She, Yaru Jiang, Yuanyuan Chen, Wenqiang Yang, Youhuai Li
Breast cancer is the leading cause of women death worldwide. Several long non-coding RNAs (lncRNAs) have been identified as oncogenes or tumor suppressors during the progression of cancers. However, the role of taurine upregulated gene (TUG1) in mediating the chemotherapy sensitivity of triple negative breast cancer (TNBC) has not been studied yet. In TNBC patients, we observed a significant decrease of TUG1 in tumor tissues compared to the normal tissues. Similarly, TUG1 expression was significantly decreased in TNBC cell lines compared with normal breast epithelial cell line and cell lines of other subtypes of breast cancer...
August 8, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Ting Yu, Yunren Fan, Yunfeng Xu, Liangdie Xu, Guangda Xu, Fangchuan Cao, Hailong Jiang
BACKGROUND: Early brain injury (EBI) plays a critical role in determining the outcome of subarachnoid hemorrhage (SAH). The present study was designed to investigate the role of EGb 761, a standardized extract of Ginkgo biloba, in SAH-induced EBI and to explore its potential mechanism of action. METHOD: A rat SAH model was established by the endovascular perforation process. Doses of 10, 50 and 100 mg/kg EGb 761 were injected intraperitoneally 2 h after SAH was induced...
August 8, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Butian Zhang, Lili Yu, Ning Han, Zhenzhen Hu, Shuang Wang, Lei Ding, Jinlan Jiang
OBJECTIVE: The purpose of this study was to find out the important lncRNA-miRNA-mRNA axis and pathway in osteosarcoma (OS) through bioinformatics analysis and verify the biological functions of lncRNA/miRNA/mRNA in OS through in vitro and in vivo assays. METHODS: The differential expression mRNAs and lncRNAs were identified through microarray analysis, and the altered pathways were identified by GSEA. The Pearson Coefficient was used to analyze the correlations between mRNAs and lncRNAs...
August 8, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Changwen Zhang, Chao Lu, Zhen Wang, Guowei Feng, E Du, Yan Liu, Li Wang, Baomin Qiao, Yong Xu, Zhihong Zhang
Antimony, one of the heavier pnictogens, is widely used in industry, and its toxicity has become a major concern. Although previous studies suggested that antimony might be a tumorigenic risk factor in several cancers, the molecular basis underlying antimony-mediated transformation remains unclear. Our results showed that the serum concentration of antimony was higher in prostate cancer specimens relative to that of benign prostate tissues, and this high serum concentration of antimony was closely associated with poorer outcome in prostate cancer patients...
August 8, 2018: Ecotoxicology and Environmental Safety
Gergely Kovács, Zsuzsanna Környei, Krisztina Tóth, Mária Baranyi, János Brunner, Máté Neubrandt, Ádám Dénes, Beáta Sperlágh
The P2X7R protein, a P2 type purinergic receptor functioning as a non-selective cation channel, is expressed in different cell types of the central nervous system in several regions of the brain. The activation of the P2X7R protein by ATP modulates excitatory neurotransmission and contributes to microglial activation, apoptosis and neuron-glia communication. Zinc is an essential micronutrient that is highly concentrated in the synaptic vesicles of glutamatergic hippocampal neurons where free zinc ions released into the synaptic cleft alter glutamatergic signal transmission...
July 30, 2018: Cell Calcium
Chong Zhang, Xue-Qi Liu, Hao-Nan Sun, Xiao-Ming Meng, Ya-Wei Bao, Hui-Ping Zhang, Fa-Ming Pan, Chao Zhang
Fibrosis is the common results from an excessive wound-healing response to chronic liver injury. Otreotide (OCT), an analogue of somatostatin, was reported to have an anti-hepatic fibrosis effect. However, its anti-fibrosis mechanisms have not been well characterized to date. The present study aimed to investigate the protective effects of OCT on carbon tetrachloride (CCl4 )-induced rat liver fibrosis and activation and proliferation of transforming growth factor-β1 (TGF-β1)-treated hepatic stellate cells (HSCs) and explore its anti-hepatofibrotic mechanisms...
August 8, 2018: International Immunopharmacology
Jing Zhao, Lili Yang, Yuhao Dai, Yingying Tang, Xiuqing Gong, Dongshu Du, Ya Cao
Protein kinases play a critical role in regulation of intracellular signal transduction, whose aberrant expression is closely associated with various dangerous human diseases. In this paper, we propose a feasible electrochemical assay of intracellular kinase by incorporating peptide nanoprobe-assisted signal labeling and signal amplification. Protein kinase A (PKA)-specific peptide P1 is self-assembled on the surface of a gold electrode, serine of which could be phosphorylated with catalysis of PKA in the presence of adenosine-5'-triphosphate (ATP)...
July 31, 2018: Biosensors & Bioelectronics
Yanxia Li, Chaoyang Jiang
Molecular imprinting is an efficient technology to create artificial receptors with antibody-like binding properties for a variety of applications such as separation and sensing. In this work, a new sensing method was introduced by combining a two-dimensional molecularly imprinted polymer (2D-MIP) film with copper oxide nanoparticles (CuO NPs) labeling for signal conversion and amplification. CuO labeling can effectively monitor the thickness of 2D-MIPs to achieve the best imprinting effect. Trypsin imprinted polymer based electrochemical sensor on 96-well microplates was constructed and a good dynamic response was observed in the range of 0...
July 31, 2018: Biosensors & Bioelectronics
Dimithree Kahanda, Naveen Singh, David A Boothman, Jason D Slinker
There is a great need to track the selectivity of anticancer drug activity and to understand the mechanisms of associated biological activity. Here we focus our studies on the specific NQO1 bioactivatable drug, ß-lapachone, which is in several Phase I clinical trials to treat human non-small cell lung, pancreatic and breast cancers. Multi-electrode chips with electrochemically-active DNA monolayers are used to track anticancer drug activity in cellular lysates and correlate cell death activity with DNA damage...
July 30, 2018: Biosensors & Bioelectronics
Weiyi Yang, Yujuan Si, Di Wang, Buhao Guo
Electrocardiogram (ECG) classification is an important process in identifying arrhythmia, and neural network models have been widely used in this field. However, these models are often disrupted by heartbeat noise and are negatively affected by skewed data. To address these problems, a novel heartbeat recognition method is presented. The aim of this study is to apply a principal component analysis network (PCANet) for feature extraction based on a noisy ECG signal. To improve the classification speed, a linear support vector machine (SVM) was applied...
August 4, 2018: Computers in Biology and Medicine
Xiaoqian Tang, Xiaoai Li, Fude Zhai, Jing Xing, Xiuzhen Sheng, Wenbin Zhan
Previous studies have demonstrated that protein tyrosine phosphorylation plays an important role in WSSV infection. In the present work, in order to further elucidate the potential role of protein tyrosine phosphorylation in white spot syndrome virus (WSSV) infection. The expression variation of tyrosine phosphorylated proteins in hemocytes of shrimp (Litopenaeus vannamei) after WSSV infection were examined by flow cytometric immunofluorescence assay (FCIFA) and enzyme linked immunosorbent assay (ELISA), and results showed that the level of protein tyrosine phosphorylation in hemocytes fluctuated significantly after WSSV infection and exhibited two peaks at 6 and 24 h post infection (hpi)...
August 8, 2018: Fish & Shellfish Immunology
Xue Zhang, Qilin Liu, Huan Zhao, Yue Hu, Cangwei Liu, Guangxing Yan, Daowei Li, Yuji Mishina, Ce Shi, Hongchen Sun
OBJECTIVE: To explore the role of a BMP type I receptor (ACVR1) in regulating periodontium development, Acvr1 was conditionally disrupted in Osterix-expressing cells. METHODS: Mandibles from both control (Acvr1 fx/+; Osterix-Cre (+)/(-)) and cKO (Acvr1 fx/-; Osterix-Cre (+)/(-)) mice at postnatal day 21 (PN21) were scanned by micro-CT, followed by decalcification and histological observations. Distributions and levels of differentiation markers of fibroblasts, osteoblasts and cementocytes in the periodontium were detected by immunohistochemical (IHC) staining...
July 31, 2018: Archives of Oral Biology
Yibin Lin, Jiahui Li, Shazhou Ye, Jiayi Chen, Yanru Zhang, Liu Wang, Yang Xi, Shizhong Bu, Xiaohui Qiu
Long non-coding RNAs (lncRNAs) have been demonstrated to perform important roles in cancer development. Previously, we have shown that lncRNA gastric cancer-associated transcript 3 (GACAT3) is overexpressed in gastric cancer and acts as a downstream target of interleukin 6/signal transducer and activator of transcription 3 (IL-6/STAT3) signaling. However, the role of GACAT3 in regulating gastric cancer cell growth remains unclear. In this study, we demonstrate that GACAT3 acts as a competing endogenous RNA of high mobility group A1 (HMGA1), a typical oncogene that is overexpressed in most types of cancer, based on a search for common miRNA-binding sites and on experiments involving in vitro cell transfection with synthesized miRNA mimics...
August 8, 2018: Gene
Zhangjun Cheng, Zhengqing Lei, Pinghua Yang, Anfeng Si, Daimin Xiang, Jiahua Zhou, Norbert Hüser
Long non-coding RNAs (lncRNAs) have been demonstrated to be involved in the development and progression of multiple cancers by previous studies. Recently, a novel lncRNA, THOR (testis-associated highly conserved oncogenic long non-coding RNA), was characterized in human cancers and shown to exhibit an oncogenic role. However, the role of THOR in hepatocellular carcinoma (HCC) is still unclear. In this study, we found that THOR was relatively highly expressed in human HCC tissues and cell lines. Notably, high THOR expression was associated with worse prognosis...
August 8, 2018: Gene
E Peverelli, E Giardino, F Mangili, D Treppiedi, R Catalano, E Ferrante, E Sala, M Locatelli, A G Lania, M Arosio, A Spada, G Mantovani
An efficient intracellular response to somatostatin analogs (SSA) in pituitary tumors requires filamin A (FLNA). Since cAMP pathway plays an important role in GH-secreting pituitary tumors pathogenesis and FLNA is phosphorylated by PKA on S2152, aim of this study was to investigate in tumoral somatotrophs the impact of cAMP pathway activation and SSA stimulation on FLNA phosphorylation and the consequences on SST2 function. We found a PKA-mediated increase (2-fold) and SST2 agonist-induced decrease (-50%) of FLNA phosphorylation in GH3, GH4C1 and primary somatotroph tumor cells...
August 8, 2018: Cancer Letters
Alexey Koval, Constant A Pieme, Emerson Ferreira Queiroz, Simone Ragusa, Kamal Ahmed, Artem Blagodatski, Jean-Luc Wolfender, Tatiana V Petrova, Vladimir L Katanaev
Triple-negative breast cancer (TNBC) and colon cancer (CC) are two stigmatic examples of poorly treatable tumors, whose progression critically depends upon hyperactivation of the Wnt signaling. Development of specific anti-Wnt inhibitors is required to develop drugs against these and other Wnt-dependent cancers. Natural products, especially plants, have been used for the treatment of various diseases from ancient times. We examined extracts from several indigenous Cameroonian herbs and tested their effects on proliferation and Wnt signaling in TNBC and CC cells...
August 8, 2018: Cancer Letters
Hanaa Ahmed-Hassan, Mohamed Sarjoon Abdul-Cader, Maha Ahmed Sabry, Eman Hamza, Mohamed Faizal Abdul-Careem
Engagement of toll-like receptor (TLR)4 ligand, lipopolysaccharide (LPS) with TLR4 in mammals activates two downstream intracellular signaling routes; the myeloid differentiation primary response gene (MyD)88 dependent and independent pathways. However, existence of the later pathway leading to production of type I interferons (IFNs) in avian species has been debated due to conflicting observations. The objective of our study was to investigate whether LPS induces type I IFN production in chicken macrophages leading to antiviral response attributable to type I IFN production...
August 8, 2018: Virus Research
Yanjun Guo, Xin Yang, Jialin He, Jingjing Liu, Shiming Yang, Hui Dong
Ca2+ -sensing receptor (CaSR), a member of G protein-coupled receptor family, is widely expressed in the vascular system, including perivascular neurons, vascular endothelial cells (VECs) and vascular smooth muscle cells (VSMCs). When stimulated, CaSR can further increase the cytosolic Ca2+ concentration ([Ca2+ ]cyt ) in two ways: intracellular Ca2+ release from endo/sarcoplasmic reticulum (ER/SR) and extracellular Ca2+ entry through Ca2+ -permeable cation channels. In endothelium, increased Ca2+ subsequently activate nitric oxide synthase (NOS) and intermediate conductance Ca2+ -activated K+ channels (IKCa), resulting in vasodilation through NOS-mediated NO release or membrane hyperpolarization...
August 8, 2018: Life Sciences
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