Stefano Negri, Yiyun Wang, Zhao Li, Qizhi Qin, Seungyong Lee, Masnsen Cherief, Jiajia Xu, Ginny Ching-Yun Hsu, Robert Joel Tower, Bradley Presson, Adam Levin, Edward McCarthy, Benjamin Levi, Aaron W James
Heterotopic ossification (HO) is a pathologic process characterized by the formation of bone tissue in extraskeletal locations. The hip is a common location of HO, especially as a complication of arthroplasty. Here, we devise a first-of-its-kind mouse model of post-surgical hip HO and validate expected cell sources of HO using several HO progenitor cell reporter lines. To induce HO, an anterolateral surgical approach to the hip was used, followed by disclocation and acetabular reaming. Animals were analyzed with high-resolution roentgenograms and micro-computed tomography, conventional histology, immunohistochemistry, and assessments of fluorescent reporter activity...
June 27, 2022: Stem Cells Translational Medicine