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https://www.readbyqxmd.com/read/29766671/point-of-care-treatment-of-focal-cartilage-defects-with-selected-chondrogenic-mesenchymal-stromal-cells-an-in-vitro-proof-of-concept-study
#1
Oliver Petters, Christian Schmidt, Christian Thuemmler, Frank Peinemann, Matthias Zscharnack, Jeremy S Somerson, Ronny M Schulz
Due to the poor self-healing capacities of cartilage, innovative approaches are a major clinical need. The use of in vitro expanded MSCs in a two-stage approach is accompanied by cost, time and personnel-intensive GMP production. A one-stage intra-operative procedure could overcome these drawbacks. The aim was to prove the feasibility of a point-of-care concept for the treatment of cartilage lesions using defined MSC subpopulations in a collagen hydrogel without prior MSC monolayer expansion. We tested four single marker candidates (MSCA-1, W4A5, CD146, CD271) for their effectiveness of separating colony-forming units of ovine MSCs via magnetic cell separation...
May 15, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29744773/cd271-cxcr7-cxcr4-and-cd133-stem-progenitor-cells-and-clinical-characteristics-of-acute-ischemic-stroke-patients
#2
Anna Gójska-Grymajło, Maciej Zieliński, Dariusz Gąsecki, Kamil Kowalczyk, Mariusz Kwarciany, Barbara Seroczyńska, Walenty M Nyka
Ischemic stroke causes mobilization of various groups of progenitor cells from bone marrow to bloodstream and this correlates with the neurological status of stroke patients. The goal of our study was to identify the activity of chosen progenitor/stem cells in the peripheral blood of acute ischemic stroke patients in the first 7 days after the incident, through associations between the levels of the cells and clinical features of the patients. Thirty-three acute ischemic stroke patients and 15 non-stroke control subjects had their venous blood collected repeatedly in order to assess the levels of the CD45-CD34 + CD271+, the CD45-CD34 + CXCR4+, the CD45-CD34 + CXCR7+, and the CD45-CD34 + CD133+ stem/progenitor cells by means of flow cytometry...
May 9, 2018: Neuromolecular Medicine
https://www.readbyqxmd.com/read/29720268/specific-markers-and-properties-of-synovial-mesenchymal-stem-cells-in-the-surface-stromal-and-perivascular-regions
#3
Mitsuru Mizuno, Hisako Katano, Yo Mabuchi, Yusuke Ogata, Shizuko Ichinose, Shizuka Fujii, Koji Otabe, Keiichiro Komori, Nobutake Ozeki, Hideyuki Koga, Kunikazu Tsuji, Chihiro Akazawa, Takeshi Muneta, Ichiro Sekiya
BACKGROUND: Synovial mesenchymal stem cells (MSCs) are an attractive cell source for cartilage and meniscus regeneration. Synovial tissue can be histologically classified into three regions; surface, stromal and perivascular region, but the localization of synovial MSCs has not been fully investigated. We identified markers specific for each region, and compared properties of MSCs derived from each region in the synovium. METHODS: The intensity of immunostaining with 19 antibodies was examined for surface, stromal, and perivascular regions of human synovium from six osteoarthritis patients...
May 2, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/29700358/premyogenic-progenitors-derived-from-human-pluripotent-stem-cells-expand-in-floating-culture-and-differentiate-into-transplantable-myogenic-progenitors
#4
Fusako Sakai-Takemura, Asako Narita, Satoru Masuda, Toshifumi Wakamatsu, Nobuharu Watanabe, Takashi Nishiyama, Ken'ichiro Nogami, Matthias Blanc, Shin'ichi Takeda, Yuko Miyagoe-Suzuki
Human induced pluripotent stem cells (hiPSCs) are a potential source for cell therapy of Duchenne muscular dystrophy. To reliably obtain skeletal muscle progenitors from hiPSCs, we treated hiPS cells with a Wnt activator, CHIR-99021 and a BMP receptor inhibitor, LDN-193189, and then induced skeletal muscle cells using a previously reported sphere-based culture. This protocol greatly improved sphere formation efficiency and stably induced the differentiation of myogenic cells from hiPS cells generated from both healthy donors and a patient with congenital myasthenic syndrome...
April 26, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29687448/stem-cell-like-populations-and-immunoregulatory-molecules-in-periodontal-granulation-tissue
#5
D A Apatzidou, C Nile, A Bakopoulou, A Konstantinidis, D F Lappin
BACKGROUND AND OBJECTIVES: Determine the presence of mesenchymal stem cells (MSCs) in healthy periodontal tissue and periodontal granulation tissue (GT) and explore associations between immuno-regulatory molecules and selected subgingival microorganisms. MATERIAL AND METHODS: Mesenchymal stem cells were isolated, propagated and characterised by flow cytometry from a region of healthy gingival tissue and inflamed GT of 10 systemically healthy non-smokers with chronic periodontitis...
April 23, 2018: Journal of Periodontal Research
https://www.readbyqxmd.com/read/29686294/development-of-new-method-to-enrich-human-ipsc-derived-renal-progenitors-using-cell-surface-markers
#6
Azusa Hoshina, Tatsuya Kawamoto, Shin-Ichi Sueta, Shin-Ichi Mae, Toshikazu Araoka, Hiromi Tanaka, Yasunori Sato, Yukiko Yamagishi, Kenji Osafune
Cell therapy using renal progenitors differentiated from human embryonic stem cells (hESCs) or induced pluripotent stem cells (hiPSCs) has the potential to significantly reduce the number of patients receiving dialysis therapy. However, the differentiation cultures may contain undifferentiated or undesired cell types that cause unwanted side effects, such as neoplastic formation, when transplanted into a body. Moreover, the hESCs/iPSCs are often genetically modified in order to isolate the derived renal progenitors, hampering clinical applications...
April 23, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29678478/immunohistochemical-cd271-expression-correlates-with-melanoma-progress-in-a-case-control-study
#7
Patricia Switten Nielsen, Rikke Riber-Hansen, Torben Steiniche
Putative cancer stem cell (CSC) markers have arisen from melanoma mouse and in vitro models, but their expression in paraffin embedded patient samples relative to clinical outcome remains largely unexplored. Rather than cells of the tumour bulk, conceivably, CSC drive tumour progression. Accordingly, complete eradication may prevent melanoma relapse. Because elevated tumour-cell proliferation is an established indicator of aggressive disease, this study aimed to investigate the correlation between melanoma recurrence and proliferation of putative CSC that express CD271, CD166, or CD20...
April 17, 2018: Pathology
https://www.readbyqxmd.com/read/29660313/the-convergent-roles-of-cd271-p75-in-neural-crest-derived-melanoma-plasticity
#8
Jennifer C Kasemeier-Kulesa, Paul M Kulesa
The embryonic microenvironment is an important source of signals that promote multipotent cells to adopt a specific fate and direct cells along distinct migratory pathways. Yet, the ability of the embryonic microenvironment to retain multipotent progenitors or reprogram de-differentiated cells is less clear. Mistakes in cell differentiation or migration often result in developmental defects and tumorigenesis, including aggressive cancers that share many characteristics with embryonic progenitor cells. This is a striking feature of the vertebrate neural crest, a multipotent and highly migratory cell population first identified by Wilhelm His (His, 1868) with the potential to metamorphose into aggressive melanoma cancer...
April 13, 2018: Developmental Biology
https://www.readbyqxmd.com/read/29597090/lobaplatin-promotes-radiosensitivity-induces-apoptosis-attenuates-cancer-stemness-and-inhibits-proliferation-through-pi3k-akt-pathway-in-esophageal-squamous-cell-carcinoma
#9
Shupei Pan, Yuchen Sun, Donghu Sui, Tian Yang, Shenbo Fu, Jizhao Wang, Beina Hui, Ruxing Xi, Chenchen He, Xiaozhi Zhang
Radiotherapy is one of the common treatments for esophageal squamous cell carcinoma (ESCC). Yet, local recurrence led by radioresistance is still not solved. Lobaplatin (LBP) is known to have powerful clinical anti-tumor activities in various tumors, but its effect in radiotherapy is rarely studied. Here we report that LBP is a promising radiosensitizer for ESCC. We treated ESCC cells with LBP and radiation, both separately and in combination. Untreated cells were set as control groups. We found that LBP inhibited ESCC cell growth and enhanced their radiosensitivity...
March 26, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29482445/single-stage-preparation-of-human-cartilage-grafts-generated-from-bone-marrow-derived-cd271-mononuclear-cells
#10
Oliver Petters, Christian Schmidt, Ralf Henkelmann, Philipp Pieroh, Gero Hütter, Bastian Marquass, Gabriela Aust, Ronny M Schulz
Due to the limited self-healing capacity of articular cartilage, innovative, regenerative approaches are of particular interest. The use of two-stage procedures utilizing in vitro-expanded mesenchymal stromal cells (MSCs) from various cell sources requires good manufacturing practice-compliant production, a process with high demands on time, staffing, and financial resources. In contrast, one- stage procedures are directly available, but need a safe enrichment of potent MSCs. CD271 is a surface marker known to marking the majority of native MSCs in bone marrow (BM)...
April 15, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29478902/tgf-%C3%AE-signaling-accelerates-senescence-of-human-bone-derived-cd271-and-ssea-4-double-positive-mesenchymal-stromal-cells
#11
Hiroshi Kawamura, Ryusuke Nakatsuka, Yoshikazu Matsuoka, Keisuke Sumide, Tatsuya Fujioka, Hiroaki Asano, Hirokazu Iida, Yoshiaki Sonoda
It is generally thought that the proliferative capacity and differentiation potential of somatic stem cells, including mesenchymal stromal/stem cells (MSCs) and hematopoietic stem cells, decline with age. We investigated the effects of aging on human bone-derived MSCs expressing CD271 and SSEA-4 (double-positive MSCs [DPMSCs]). The percentages of DPMSCs in bone tissue decreased significantly with age. The DPMSCs from elderly patients (old DPMSCs) showed cellular senescence, which was evidenced by low growth potential, high senescence-associated β-galactosidase activity, and elevated p16 and p21 CDK inhibitor levels...
March 13, 2018: Stem Cell Reports
https://www.readbyqxmd.com/read/29420557/capillary-networks-and-follicular-marginal-zones-in-human-spleens-three-dimensional-models-based-on-immunostained-serial-sections
#12
Birte S Steiniger, Christine Ulrich, Moritz Berthold, Michael Guthe, Oleg Lobachev
We have reconstructed small parts of capillary networks in the human splenic white pulp using serial sections immunostained for CD34 alone or for CD34 and CD271. The three-dimensional (3D) models show three types of interconnected networks: a network with very few long capillaries inside the white pulp originating from central arteries, a denser network surrounding follicles plus periarterial T-cell regions and a network in the red pulp. Capillaries of the perifollicular network and the red pulp network have open ends...
2018: PloS One
https://www.readbyqxmd.com/read/29358574/publisher-correction-the-low-affinity-neurotrophin-receptor-cd271-regulates-phenotype-switching-in-melanoma
#13
Gaetana Restivo, Johanna Diener, Phil F Cheng, Gregor Kiowski, Mario Bonalli, Thomas Biedermann, Ernst Reichmann, Mitchell P Levesque, Reinhard Dummer, Lukas Sommer
The originally published version of this Article was updated shortly after publication to add the words 'The' and 'affinity' to the title, following their inadvertent removal during the production process. This has now been corrected in both the PDF and HTML versions of the Article.
January 22, 2018: Nature Communications
https://www.readbyqxmd.com/read/29296636/characterization-of-lymphopenia-in-patients-with-ms-treated-with-dimethyl-fumarate-and-fingolimod
#14
Maryam Nakhaei-Nejad, David Barilla, Chieh-Hsin Lee, Gregg Blevins, Fabrizio Giuliani
Objective: Lymphopenia is a common occurrence of disease-modifying therapies (DMTs) for relapsing-remitting MS (RRMS). The aim of this study was to dissect the prevalence of various lymphocyte subsets in patients with RRMS treated with 2 DMTs commonly associated with lymphopenia, dimethyl fumarate (DMF), and fingolimod (FTY). Methods: Multicolor flow cytometry and multiplex assays were used to identify up to 50 lymphocyte subpopulations and to examine the expression of multiple cytokines in selected patients...
March 2018: Neurology® Neuroimmunology & Neuroinflammation
https://www.readbyqxmd.com/read/29224250/multiple-autologous-bone-marrow-derived-cd271-mesenchymal-stem-cell-transplantation-overcomes-drug-resistant-epilepsy-in-children
#15
Olga Milczarek, Danuta Jarocha, Anna Starowicz-Filip, Stanislaw Kwiatkowski, Bogna Badyra, Marcin Majka
There is a need among patients suffering from drug-resistant epilepsy (DRE) for more efficient and less toxic treatments. The objective of the present study was to assess the safety, feasibility, and potential efficacy of autologous bone marrow cell transplantation in pediatric patients with DRE. Two females and two males (11 months to 6 years) were enrolled and underwent a combined therapy consisting of autologous bone marrow nucleated cells (BMNCs) transplantation (intrathecal: 0.5 × 109 ; intravenous: 0...
January 2018: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29215016/low-neurotrophin-receptor-cd271-regulates-phenotype-switching-in-melanoma
#16
Gaetana Restivo, Johanna Diener, Phil F Cheng, Gregor Kiowski, Mario Bonalli, Thomas Biedermann, Ernst Reichmann, Mitchell P Levesque, Reinhard Dummer, Lukas Sommer
Cutaneous melanoma represents the most fatal skin cancer due to its high metastatic capacity. According to the "phenotype switching" model, the aggressive nature of melanoma cells results from their intrinsic potential to dynamically switch from a high-proliferative/low-invasive to a low-proliferative/high-invasive state. Here we identify the low affinity neurotrophin receptor CD271 as a key effector of phenotype switching in melanoma. CD271 plays a dual role in this process by decreasing proliferation, while simultaneously promoting invasiveness...
December 7, 2017: Nature Communications
https://www.readbyqxmd.com/read/29208672/cd271-confers-an-invasive-and-metastatic-phenotype-of-head-and-neck-squamous-cell-carcinoma-through-the-upregulation-of-slug
#17
Man Ki Chung, Young Ho Jung, Joon Kyoo Lee, Soo Youn Cho, Oihana Murillo-Sauca, Ravindra Uppaluri, June Ho Shin, John B Sunwoo
Purpose: Head and neck squamous cell carcinoma (HNSCC) is comprised of heterogeneous populations of cells, and CD271 (NGFR; p75NTR) has been associated with a tumor-initiating cell subpopulation. This study assessed the role of CD271 in modulating metastatic behavior in HNSCC. Experimental Design: CD271 was overexpressed in murine and human oral squamous cell carcinoma cells to assess the impact of CD271 activation on the invasive and metastatic phenotype of these cells, using in vitro and orthotopic in vivo modeling...
February 1, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29151581/human-pbmc-transferred-murine-mhc-class-i-ii-deficient-nog-mice-enable-long-term-evaluation-of-human-immune-responses
#18
Tomonori Yaguchi, Asuka Kobayashi, Takashi Inozume, Kenji Morii, Haruna Nagumo, Hiroshi Nishio, Takashi Iwata, Yuyo Ka, Ikumi Katano, Ryoji Ito, Mamoru Ito, Yutaka Kawakami
Immunodeficient mice engrafted with human peripheral blood cells are promising tools for in vivo analysis of human patient individual immune responses. However, when human peripheral blood mononuclear cells (PBMCs) are transferred into NOG (NOD/Shi-scid, IL-2rg(null)) mice, severe graft versus host disease (GVHD) hinders long term detailed analysis. Administration of human PBMCs into newly developed murine MHC class I- and class II-deficient NOG (NOG-dKO; NOG- Iab, B2m-double-knockout) mice showed sufficient engraftment of human immune cells with little sign of GVHD...
November 20, 2017: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/29150821/cd271-mediates-proliferation-and-differentiation-of-epidermal-stem-cells-to-support-cutaneous-burn-wound-healing
#19
Min Zhang, Yongqian Cao, Xiaohong Li, Lizhi Hu, Sahbi Khaled Taieb, Xiaolong Zhu, Jing Zhang, Yongqiang Feng, Ran Zhao, Mingqing Wang, Wenjun Xue, Zhanjie Yang, Yibing Wang
Burn wounds can significantly reduce the quality of life of patients with respect to their physiology and psychology and can even threaten their lives. Many treatments have been proposed, including stem cell therapy but no effective method can as yet cure such damage. Our study highlights the role of Cd271 in epidermal stem cells (eSC) during the healing of burn wounds. The expression of Cd271 increases together with burn wound healing. Injection of Cd271-over-expressing eSC into wounds promotes the healing rate in a mouse burn model...
February 2018: Cell and Tissue Research
https://www.readbyqxmd.com/read/29125884/comparative-characterization-of-cd271-and-cd271-subpopulations-of-cd34-human-adipose-derived-stromal-cells
#20
Liziane R Beckenkamp, Lucas E B Souza, Fernanda U F Melo, Carolina H Thomé, Daniele A R Magalhães, Patrícia V B Palma, Dimas T Covas
Adipose-derived stromal/stem cells (ASCs) are promising candidates for cell-based therapies. However, the lack of markers able to unequivocally identify these cells, the differential expression of cell surface molecules among stromal progenitors from different tissues and cellular alterations caused by culture are phenomena that need to be comprehensively addressed in order to improve ASC purification and consequently refine our knowledge about their function and therapeutic efficiency. In this study, we investigated the potential of CD271, a marker used for purification of bone marrow-derived mesenchymal stem cells, on enriching ASCs from CD34+ stromal cells of human adipose tissue...
May 2018: Journal of Cellular Biochemistry
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