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https://www.readbyqxmd.com/read/29296636/characterization-of-lymphopenia-in-patients-with-ms-treated-with-dimethyl-fumarate-and-fingolimod
#1
Maryam Nakhaei-Nejad, David Barilla, Chieh-Hsin Lee, Gregg Blevins, Fabrizio Giuliani
Objective: Lymphopenia is a common occurrence of disease-modifying therapies (DMTs) for relapsing-remitting MS (RRMS). The aim of this study was to dissect the prevalence of various lymphocyte subsets in patients with RRMS treated with 2 DMTs commonly associated with lymphopenia, dimethyl fumarate (DMF), and fingolimod (FTY). Methods: Multicolor flow cytometry and multiplex assays were used to identify up to 50 lymphocyte subpopulations and to examine the expression of multiple cytokines in selected patients...
March 2018: Neurology® Neuroimmunology & Neuroinflammation
https://www.readbyqxmd.com/read/29224250/multiple-autologous-bone-marrow-derived-cd271-mesenchymal-stem-cell-transplantation-overcomes-drug-resistant-epilepsy-in-children
#2
Olga Milczarek, Danuta Jarocha, Anna Starowicz-Filip, Stanislaw Kwiatkowski, Bogna Badyra, Marcin Majka
There is a need among patients suffering from drug-resistant epilepsy (DRE) for more efficient and less toxic treatments. The objective of the present study was to assess the safety, feasibility, and potential efficacy of autologous bone marrow cell transplantation in pediatric patients with DRE. Two females and two males (11 months to 6 years) were enrolled and underwent a combined therapy consisting of autologous bone marrow nucleated cells (BMNCs) transplantation (intrathecal: 0.5 × 109 ; intravenous: 0...
December 10, 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/29215016/low-neurotrophin-receptor-cd271-regulates-phenotype-switching-in-melanoma
#3
Gaetana Restivo, Johanna Diener, Phil F Cheng, Gregor Kiowski, Mario Bonalli, Thomas Biedermann, Ernst Reichmann, Mitchell P Levesque, Reinhard Dummer, Lukas Sommer
Cutaneous melanoma represents the most fatal skin cancer due to its high metastatic capacity. According to the "phenotype switching" model, the aggressive nature of melanoma cells results from their intrinsic potential to dynamically switch from a high-proliferative/low-invasive to a low-proliferative/high-invasive state. Here we identify the low affinity neurotrophin receptor CD271 as a key effector of phenotype switching in melanoma. CD271 plays a dual role in this process by decreasing proliferation, while simultaneously promoting invasiveness...
December 7, 2017: Nature Communications
https://www.readbyqxmd.com/read/29208672/cd271-confers-an-invasive-and-metastatic-phenotype-of-head-and-neck-squamous-cell-carcinoma-through-the-upregulation-of-slug
#4
Man Ki Chung, Young Ho Jung, Joon Kyoo Lee, Soo Youn Cho, Oihana J Murillo-Sauca, Ravindra Uppaluri, June Ho Shin, John B Sunwoo
PURPOSE: Head and neck squamous cell carcinoma (HNSCC) is comprised of heterogeneous populations of cells, and CD271 (NGFR; p75NTR) has been associated with a tumor-initiating cell subpopulation. This study assessed the role of CD271 in modulating metastatic behavior in HNSCC. EXPERIMENTAL DESIGN: CD271 was overexpressed in murine and human oral squamous cell carcinoma cells to assess the impact of CD271 activation on the invasive and metastatic phenotype of these cells, using in vitro and orthotopic in vivo modeling...
December 5, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29151581/human-pbmc-transferred-murine-mhc-class-i-ii-deficient-nog-mice-enable-long-term-evaluation-of-human-immune-responses
#5
Tomonori Yaguchi, Asuka Kobayashi, Takashi Inozume, Kenji Morii, Haruna Nagumo, Hiroshi Nishio, Takashi Iwata, Yuyo Ka, Ikumi Katano, Ryoji Ito, Mamoru Ito, Yutaka Kawakami
Immunodeficient mice engrafted with human peripheral blood cells are promising tools for in vivo analysis of human patient individual immune responses. However, when human peripheral blood mononuclear cells (PBMCs) are transferred into NOG (NOD/Shi-scid, IL-2rg(null)) mice, severe graft versus host disease (GVHD) hinders long term detailed analysis. Administration of human PBMCs into newly developed murine MHC class I- and class II-deficient NOG (NOG-dKO; NOG- Iab, B2m-double-knockout) mice showed sufficient engraftment of human immune cells with little sign of GVHD...
November 20, 2017: Cellular & Molecular Immunology
https://www.readbyqxmd.com/read/29150821/cd271-mediates-proliferation-and-differentiation-of-epidermal-stem-cells-to-support-cutaneous-burn-wound-healing
#6
Min Zhang, Yongqian Cao, Xiaohong Li, Lizhi Hu, Sahbi Khaled Taieb, Xiaolong Zhu, Jing Zhang, Yongqiang Feng, Ran Zhao, Mingqing Wang, Wenjun Xue, Zhanjie Yang, Yibing Wang
Burn wounds can significantly reduce the quality of life of patients with respect to their physiology and psychology and can even threaten their lives. Many treatments have been proposed, including stem cell therapy but no effective method can as yet cure such damage. Our study highlights the role of Cd271 in epidermal stem cells (eSC) during the healing of burn wounds. The expression of Cd271 increases together with burn wound healing. Injection of Cd271-over-expressing eSC into wounds promotes the healing rate in a mouse burn model...
November 18, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/29125884/comparative-characterization-of-cd271-and-cd271-subpopulations-of-cd34-human-adipose-derived-stromal-cells
#7
Liziane Raquel Beckenkamp, Lucas Eduardo Botelho Souza, Fernanda Ursoli Ferreira Melo, Carolina Hassibe Thomé, Daniele Aparecida Rosa Magalhães, Patrícia Viana Bonini Palma, Dimas Tadeu Covas
INTRODUCTION: Adipose-derived stromal/stem cells (ASCs) are promising candidates for cell-based therapies. However, the lack of markers able to unequivocally identify these cells, the differential expression of cell surface molecules among stromal progenitors from different tissues and cellular alterations caused by culture are phenomena that need to be comprehensively addressed in order to improve ASC purification and consequently refine our knowledge about their function and therapeutic efficiency...
November 10, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29118440/native-joint-resident-mesenchymal-stem-cells-for-cartilage-repair-in-osteoarthritis
#8
REVIEW
Dennis McGonagle, Thomas G Baboolal, Elena Jones
The role of native (not culture-expanded) joint-resident mesenchymal stem cells (MSCs) in the repair of joint damage in osteoarthritis (OA) is poorly understood. MSCs differ from bone marrow-residing haematopoietic stem cells in that they are present in multiple niches in the joint, including subchondral bone, cartilage, synovial fluid, synovium and adipose tissue. Research in experimental models suggests that the migration of MSCs adjacent to the joint cavity is crucial for chonodrogenesis during embryogenesis, and also shows that synovium-derived MSCs might be the primary drivers of cartilage repair in adulthood...
December 2017: Nature Reviews. Rheumatology
https://www.readbyqxmd.com/read/28967641/large-scale-expansion-and-characterization-of-human-adult-neural-crest-derived-multipotent-stem-cells-from-hair-follicle-for-regenerative-medicine-applications
#9
R G Vasyliev, A E Rodnichenko, O S Gubar, A V Zlatska, I M Gordiienko, S N Novikova, D O Zubov
AIM: The purpose of this work was to obtain, multiply and characterize the adult neural crest-derived multipotent stem cells from human hair follicle for their further clinical use. MATERIALS AND METHODS: Adult neural crest-derived multipotent stem cells were obtained from human hair follicle by explant method and were expanded at large-scale up to a clinically significant number. The resulted cell cultures were examined by flow cytometry and immunocytochemical analysis...
September 2017: Experimental Oncology
https://www.readbyqxmd.com/read/28967640/endometrial-stromal-cells-isolation-expansion-morphological-and-functional-properties
#10
A V Zlatska, A E Rodnichenko, O S Gubar, D O Zubov, S N Novikova, R G Vasyliev
AIM: We aimed to study biological properties of human endometrial stromal cells in vitro. MATERIALS AND METHODS: The endometrium samples (n = 5) were obtained by biopsy at the first phase of the menstrual cycle from women with endometrial hypoplasia. In all cases, a voluntary written informed consent was obtained from the patients. Endometrial fragments were dissociated by enzymatic treatment. The cells were cultured in DMEM/F12 supplemented with 10% FBS, 2 mМ L-glutamine and 1 ng/ml FGF-2 in a multi-gas incubator at 5% CO2 and 5% O2...
September 2017: Experimental Oncology
https://www.readbyqxmd.com/read/28941240/dynamic-changes-in-brain-mesenchymal-perivascular-cells-associate-with-multiple-sclerosis-disease-duration-active-inflammation-and-demyelination
#11
Ellen Iacobaeus, Rachael V Sugars, Anton Törnqvist Andrén, Jessica J Alm, Hong Qian, Janek Frantzen, Jia Newcombe, Kanar Alkass, Henrik Druid, Matteo Bottai, Matias Röyttä, Katarina Le Blanc
Vascular changes, including blood brain barrier destabilization, are common pathological features in multiple sclerosis (MS) lesions. Blood vessels within adult organs are reported to harbor mesenchymal stromal cells (MSCs) with phenotypical and functional characteristics similar to pericytes. We performed an immunohistochemical study of MSCs/pericytes in brain tissue from MS and healthy persons. Post-mortem brain tissue from patients with early progressive MS (EPMS), late stage progressive MS (LPMS), and healthy persons were analyzed for the MSC and pericyte markers CD146, platelet-derived growth factor receptor beta (PDGFRβ), CD73, CD271, alpha-smooth muscle actin, and Ki67...
October 2017: Stem Cells Translational Medicine
https://www.readbyqxmd.com/read/28925497/the-feasibility-of-the-cd271-and-cd271-mesenchymal-stromal-cell-enrichment-toward-nucleus-pulposus-like-cells
#12
Katarzyna Jezierska-Wozniak, Monika Barczewska, Aleksandra Habich, Pawel Wojtacha, Wanda Badowska, Wojciech Maksymowicz, Joanna Wojtkiewicz
INTRODUCTION: Factors promoting nerve cell ingrowth are considered responsible for chronic back pain resulting from the intervertebral disc degeneration (IDD). One of the recent exploratory IDD treatments is stem cell transplantation therapy. The CD271 (low-affinity nerve growth factor receptor) has been identified as a marker of the most homogeneous mesenchymal stem cell (MSC) subset. It is capable of promoting differentiation along adipogenic, osteogenic and chondrogenic lineages and producing significantly higher levels of cytokines as compared to the total population of plastic adherence-mesenchymal stem cells (PA-MSCs)...
September 19, 2017: Folia Histochemica et Cytobiologica
https://www.readbyqxmd.com/read/28884113/in-vitro-culture-of-na%C3%A3-ve-human-bone-marrow-mesenchymal-stem-cells-a-stemness-based-approach
#13
REVIEW
Bidisha Pal, Bikul Das
Human bone marrow derived mesenchymal stem cells (BM-MSCs) resides in their niches in close proximity to hematopoietic stem cells (HSCs). These naïve MSCs have tremendous potential in regenerative therapeutics, and may also be exploited by cancer and infectious disease agents. Hence, it is important to study the physiological and pathological roles of naïve MSC. However, our knowledge of naïve MSCs is limited by lack of appropriate isolation and in vitro culture methods. Established culture methods use serum rich media, and serial passaging for retrospective isolation of MSCs...
2017: Frontiers in Cell and Developmental Biology
https://www.readbyqxmd.com/read/28852061/cd271-determines-migratory-properties-of-melanoma-cells
#14
Josefine Radke, Florian Roßner, Torben Redmer
Melanoma cell expression of the nerve growth factor receptor CD271 is associated with stem-like properties. However, the contributing role of the receptor in melanoma cell migration is elusive. Here, we explored extracranial (skin, soft tissue, lymph node and liver, n = 13) and matched brain metastases (BM, n = 12) and observed a heterogeneous distribution of phenotypically distinct subsets of CD271(+) cells. In addition, we observed that CD271 expression gradually rises along with melanoma progression and metastasis by exploration of publicly available expression data of nevi, primary melanoma (n = 31) and melanoma metastases (n = 54)...
August 29, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28734835/migration-ability-and-toll-like-receptor-expression-of-human-mesenchymal-stem-cells-improves-significantly-after-three-dimensional-culture
#15
Panpan Zhou, Zilin Liu, Xue Li, Bing Zhang, Xiaoyuan Wang, Jing Lan, Qing Shi, Dong Li, Xiuli Ju
While the conventional two-dimensional (2D) culture protocol is well accepted for the culture of mesenchymal stem cells (MSCs), this method fails to recapitulate the in vivo native three-dimensional (3D) cellular microenvironment, and may result in phenotypic changes, and homing and migration capacity impairments. MSC preparation in 3D culture systems has been considered an attractive preparatory and delivery method recently. We seeded human umbilical cord-derived MSCs (hUCMSCs) in a 3D culture system with porcine acellular dermal matrix (PADM), and investigated the phenotypic changes, the expression changes of some important receptors, including Toll-like receptors (TLRs) and C-X-C chemokine receptor type 4 (CXCR4) when hUCMSCs were transferred from 2D to 3D systems, as well as the alterations in in vivo homing and migration potential...
September 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28726209/characteristics-of-mel-ibr-melanoma-line-subclone-after-treatment-with-chicken-embryo-extract
#16
N M Suraeva, L F Morozova, O O Ryabaya, Yu A Khochenkova, A V Samoilov, O S Burova, N V Golubtsova, A E Barmashov, M A Baryshnikova
We continue analysis of the phenotype of human melanoma cell Mel Ibr subclone obtained previously by treatment of the parental cell line by chicken embryo extract. The present study is focused on detection of markers of epithelial-mesenchymal transition that determine enhanced metastatic and invasive potential of malignant tumors of various locations. Analysis of the expression of E-cadherin and vimentin genes in the subclone and parental cells detected activation of epithelial-mesenchymal transition in the subclone...
June 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28706966/data-on-the-fate-of-macs%C3%A2-microbeads-intramyocardially-co-injected-with-stem-cell-products
#17
Paula Müller, Ralf Gaebel, Heiko Lemcke, Gustav Steinhoff, Robert David
The data presented in this article are related to the research article "Intramyocardial Fate and Effect of Iron Nanoparticles co-injected with MACS® purified Stem Cell Products" (Müller et al., 2017) [1]. This article complements the cellular localization of superparamagnetic iron dextran particles (MACS® MicroBeads) used for magnetic activated cell sorting (MACS®). Data evaluate the time-dependent detachment of these nanoparticles from CD133(+) haematopoietic stem cells (HSCs) and CD271(+) mesenchymal stem cells (MSCs)...
August 2017: Data in Brief
https://www.readbyqxmd.com/read/28639326/bone-marrow-hematons-an-access-point-to-the-human-hematopoietic-niche
#18
Alexandre Janel, Juliette Berger, Céline Bourgne, Richard Lemal, Nathalie Boiret-Dupré, Frédérique Dubois-Galopin, Pierre Déchelotte, Charlotte Bothorel, Eric Hermet, Sara Chabi, Jacques-Olivier Bay, Céline Lambert, Bruno Pereira, Françoise Pflumio, Rima Haddad, Marc G Berger
To understand the complex interactions between hematopoietic stem cells and the bone marrow niche, a human experimental model is needed. Our hypothesis is that hematons are an appropriate ex vivo model of human bone marrow. We analyzed the hierarchical hematopoietic cell content and the tissue organization of single hematons from healthy donors. Most (>90%) hematons contained precursors of all cell lineages, myeloid progenitors, and LTC-ICs without preferential commitment. Approximately, half of the hematons could generate significant levels of lympho-myeloid hematopoiesis after transplantation in an NSG mouse model, despite the low absolute numbers of transplanted CD34(+) cells...
October 2017: American Journal of Hematology
https://www.readbyqxmd.com/read/28633632/antagonizing-mir-455-3p-inhibits-chemoresistance-and-aggressiveness-in-esophageal-squamous-cell-carcinoma
#19
Aibin Liu, Jinrong Zhu, Geyan Wu, Lixue Cao, Zhanyao Tan, Shuxia Zhang, Lili Jiang, Jueheng Wu, Mengfeng Li, Libing Song, Jun Li
BACKGROUND: The plasticity of cancer stem cells (CSCs)/tumor-initiating cells (T-ICs) suggests that multiple CSC/T-IC subpopulations exist within a tumor and that multiple oncogenic pathways collaborate to maintain the CSC/T-IC state. Here, we aimed to identify potential therapeutic targets that concomitantly regulate multiple T-IC subpopulations and CSC/T-IC-associated pathways. METHODS: A chemoresistant patient-derived xenograft (PDX) model of human esophageal squamous cell carcinoma (ESCC) was employed to identify microRNAs that contribute to ESCC aggressiveness...
June 21, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28575019/clinicopathologic-significance-of-the-cxcl1-cxcr2-axis-in-the-tumor-microenvironment-of-gastric-carcinoma
#20
Hiroaki Kasashima, Masakazu Yashiro, Hirohisa Nakamae, Go Masuda, Haruhito Kinoshita, Tamami Morisaki, Tatsunari Fukuoka, Tsuyoshi Hasegawa, Takahiko Nakane, Masayuki Hino, Kosei Hirakawa, Masaichi Ohira
PURPOSE: It was reported that the chemokine (C-X-C motif) ligand 1 (CXCL1) from cancer cells stimulated the recruitment of bone marrow-derived mesenchymal cells (BM-MCs) into tumor stroma via chemokine (C-X-C motif) receptor 2 (CXCR2) signaling. We conducted this retrospective study to determine the clinicopathologic significance of the CXCL1-CXCR2 axis in human gastric cancer. METHODS: The correlations between the clinicopathological features of 270 primary gastric carcinomas and CXCL1 in cancer cells and CXCR2 in stromal cells were analyzed in immunohistochemical studies...
2017: PloS One
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