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https://www.readbyqxmd.com/read/28738664/bomb-swab-can-trace-explosive-particle-sampling-and-detection-be-improved
#1
Danny Fisher, Raya Zach, Yossef Matana, Paz Elia, Shiran Shustack, Yarden Sharon, Yehuda Zeiri
The marked increase in international terror in recent years requires the development of highly efficient methods to detect trace amounts of explosives at airports, border crossings and check points. The preferred analytical method worldwide is the ion mobility spectrometry (IMS) that is capable of detecting most explosives at the nano-gram level. Sample collection for the IMS analysis is based on swabbing of a passenger's belongings to collect possible explosive residues. The present study examines a wide range of issues related to swab-based particle collection and analysis, in the hope of gaining deeper understanding into this technique that will serve to improve the detection process...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738600/quantification-of-intercellular-adhesion-forces-measured-by-fluid-force-microscopy
#2
Noa Cohen, Saheli Sarkar, Evangelia Hondroulis, Pooja Sabhachandani, Tania Konry
The mechanics of cancer cell adhesion to its neighboring cells, homotypic or heterotypic, have significant impact on tumor progression and metastasis. Intercellular adhesion has been quantified previously using atomic force microscopy-based methods. Here we show the feasibility of the recently developed fluidic force microscopy (FluidFM) to measure adhesive forces exerted by breast cancer cells. Multiple cell pairs were assessed at precisely controlled, increasing contact durations by pressure-dependent immobilization of a cell at the probe tip...
November 1, 2017: Talanta
https://www.readbyqxmd.com/read/28738356/thermal-preconditioning-may-prevent-tendon-adhesion-by-up-regulating-hsp72-in-rats
#3
Yang Tan, Qin-Fen Wu, Qiang Wu, Xin-Ti Tan, Liao-Bin Chen, Xin Wang
BACKGROUND/AIMS: The study aims to determine the effects of thermal preconditioning on tendon adhesion by regulating the expression of heat shock protein 72 (HSP72) in rat models. METHODS: Sixty male Wistar rats were collected and randomly assigned into the thermal preconditioning and control groups. During the 4th and 8th weeks following surgery, 15 rats were sacrificed in each period respectively, and their tendon adhesion was observed and evaluated. Biomechanical testing was performed to measure the tensile strength and gliding distance of tendons...
July 24, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28738291/inflammation-responsive-self-regulated-drug-release-from-ultrathin-hydrogel-coating
#4
Min He, Qian Wang, Zhenqiang Shi, Yi Xie, Weifeng Zhao, Changsheng Zhao
Heterotopic ossification(HO) is a potential severe complication after many biomaterial implanting surgeries, and the inflammation environment caused by the implanting-associated infections is considered as the main nosogenesis. Herein, an inflammation-responsive drug release system was designed by chemically conjugating indometacin (via ester group) onto hydrogel coating to realize local self-regulated drug release to prevent HO. In our strategy, poly(3-mercaptopropyl)trimethoxysilane-co-acrylic acrylate and polyvinyl alcohol (providing anchoring sites for drug molecules) were firstly synthesized and functionalized with ene-groups, then a hydrogel layer was formed and covalently attached onto thiol-modified substrate via thiol-ene click chemistry, followed by grafting indometacin...
July 18, 2017: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/28738269/an-anti-inflammatory-effect-of-red-microalga-polysaccharides-in-coronary-artery-endothelial-cells
#5
Oshrat Levy-Ontman, Mahmoud Huleihel, Rachel Hamias, Talya Wolak, Esther Paran
BACKGROUND AND AIMS: Polysaccharides (PSs) produced by the red microalga Porphyridium sp. were reported to exhibit anti-inflammatory bioactivities in the human skin. The primary goal of the present research was to assess whether PSs attenuate inflammatory processes by interfering with tumour necrosis factor-alpha (TNF-α)-induced inflammation, in human coronary artery endothelial cells (HCAECs). METHODS: Functional and inflammatory markers were quantified in TNF-α-stimulated HCAECs, with and without pre-treatment with PSs...
July 16, 2017: Atherosclerosis
https://www.readbyqxmd.com/read/28737930/self-collapse-lithography
#6
Chuanzhen Zhao, Xiaobin Xu, Qing Yang, Tianxing Man, Steven Jonas, Jeffrey J Schwartz, Anne M Andrews, Paul S Weiss
We report a facile, high-throughput soft lithography process that utilizes nanoscale channels formed naturally at the edges of microscale relief features on soft, elastomeric stamps. Upon contact with self-assembled monolayer (SAM) functionalized substrates, the roof of the stamp collapses, resulting in the selective removal of SAM molecules via a chemical lift-off process. With this technique, which we call self-collapse lithography (SCL), sub-30-nm patterns were achieved readily using masters with microscale features prepared by conventional photolithography...
July 24, 2017: Nano Letters
https://www.readbyqxmd.com/read/28737896/interfacial-shear-strength-and-adhesive-behavior-of-silk-ionomer-surfaces
#7
Sunghan Kim, Ren D Geryak, Shuaidi Zhang, Ruilong Ma, Rossella Calabrese, David L Kaplan, Vladimir V Tsukruk
The interfacial shear strength between different layers in multi-layered structures of layer-by-layer (LbL) microcapsules is a crucial mechanical property to ensure their robustness. In this work, we investigated the interfacial shear strength of modified silk fibroin ionomers utilized in LbL shells, an ionic-cationic pair with complimentary ionic pairing, (SF)-poly-L-glutamic acid (Glu) and SF- poly-L-lysine (Lys) and a complementary pair with partially screened Coulombic interactions due to the presence of poly(ethylene glycol) (PEG) segments, SF-Glu/SF-Lys[PEG] pair...
July 24, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28737725/optimization-of-the-silver-nanoparticles-peald-process-on-the-surface-of-1-d-titania-coatings
#8
Aleksandra Radtke, Tomasz Jędrzejewski, Wiesław Kozak, Beata Sadowska, Marzena Więckowska-Szakiel, Ewa Talik, Maarit Mäkelä, Markku Leskelä, Piotr Piszczek
Plasma enhanced atomic layer deposition (PEALD) of silver nanoparticles on the surface of 1-D titania coatings, such as nanotubes (TNT) and nanoneedles (TNN), has been carried out. The formation of TNT and TNN layers enriched with dispersed silver particles of strictly defined sizes and the estimation of their bioactivity was the aim of our investigations. The structure and the morphology of produced materials were determined using X-ray photoelectron spectroscopy (XPS) and scanning electron miscroscopy (SEM)...
July 24, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28737509/astrocytic-tight-junctions-control-inflammatory-cns-lesion-pathogenesis
#9
Sam Horng, Anthony Therattil, Sarah Moyon, Alexandra Gordon, Karla Kim, Azeb Tadesse Argaw, Yuko Hara, John N Mariani, Setsu Sawai, Per Flodby, Edward D Crandall, Zea Borok, Michael V Sofroniew, Candice Chapouly, Gareth R John
Lesions and neurologic disability in inflammatory CNS diseases such as multiple sclerosis (MS) result from the translocation of leukocytes and humoral factors from the vasculature, first across the endothelial blood-brain barrier (BBB) and then across the astrocytic glia limitans (GL). Factors secreted by reactive astrocytes open the BBB by disrupting endothelial tight junctions (TJs), but the mechanisms that control access across the GL are unknown. Here, we report that in inflammatory lesions, a second barrier composed of reactive astrocyte TJs of claudin 1 (CLDN1), CLDN4, and junctional adhesion molecule A (JAM-A) subunits is induced at the GL...
July 24, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/28737334/-barriers-to-tuberculosis-awareness-and-screening-a-qualitative-study-in-a-french-department
#10
Séverine Carillon, Annabel Desgrées du Loû
<ce:para>The incidence of tuberculosis in Seine-Saint-Denis is considerably higher than the national average. The north-west part of the department is particularly concerned. Screening teams encounter difficulties communicating on the disease, reaching the populations concerned and obtaining their adhesion to the screening proposal. The objective of this study was to identify and elucidate the obstacles to tuberculosis prevention and screening.</ce:para> <ce:para>A qualitative study was conducted based on observation of screening actions in Seine-Saint-Denis and semi-directive interviews with health professionals in charge of screening, community representatives and associations, and individuals from the population concerned...
April 27, 2017: Santé Publique: Revue Multidisciplinaire Pour la Recherche et L'action
https://www.readbyqxmd.com/read/28737182/micro-and-nano-patterned-elastin-like-polypeptide-hydrogels-for-stem-cell-culture
#11
A Paul, M Stührenberg, S Chen, D Rhee, W-K Lee, T W Odom, S C Heilshorn, A Enejder
We show that submicron-sized patterns can be imprinted into soft, recombinant-engineered protein hydrogels (here elastin-like proteins, ELP) by transferring wavy patterns from polydimethylsiloxane (PDMS) molds. The high-precision topographical tunability of the relatively stiff PDMS is translated to a bio-responsive, soft material, enabling topographical cell response studies at elastic moduli matching those of tissues. Aligned and unaligned wavy patterns with mold periodicities of 0.24-4.54 μm were imprinted and characterized by coherent anti-Stokes Raman scattering and atomic force microscopy...
July 24, 2017: Soft Matter
https://www.readbyqxmd.com/read/28736979/in-vitro-characteristics-of-lactobacillus-spp-strains-isolated-from-the-chicken-digestive-tract-and-their-role-in-the-inhibition-of-campylobacter-colonization
#12
Patrycja A Kobierecka, Agnieszka K Wyszyńska, Tamara Aleksandrzak-Piekarczyk, Maciej Kuczkowski, Anna Tuzimek, Wioletta Piotrowska, Adrian Górecki, Iwona Adamska, Alina Wieliczko, Jacek Bardowski, Elżbieta K Jagusztyn-Krynicka
Campylobacter jejuni/coli infections are the leading cause of bacterial diarrheal illnesses in humans. Many epidemiological studies indicate that improperly prepared meat from chickens that carry a high load of Campylobacter in their intestinal tracts is the key source of human infections. LAB, mainly members of the Lactococcus and Lactobacillus genera, increasingly have been tested as vehicles for the delivery of heterologous bacterial or viral antigens to animal mucosal immune systems. Thus, the objective of this study was to isolate, identify, and characterize Lactobacillus spp...
July 24, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28736604/comparison-between-three-glass-fiber-post-cementation-techniques
#13
Guido Migliau, Luca Piccoli, Stefano Di Carlo, Giorgio Pompa, Laith Konstantinos Besharat, Marco Dolci
OBJECTIVE: The aim of this experimental study was to compare the traditional cement systems with those of the latest generation, to assess if indeed these could represent of viable substitutes in the cementation of indirect restorations, and in the specific case of endodontic posts. METHODS: The assessment of the validity of the cementing methods was performed according to the test of the push-out, conducted on sections obtained from the roots of treated teeth. The samples were divided into three groups...
January 2017: Annali di Stomatologia
https://www.readbyqxmd.com/read/28736601/classification-review-of-dental-adhesive-systems-from-the-iv-generation-to-the-universal-type
#14
REVIEW
Eshrak Sofan, Afrah Sofan, Gaspare Palaia, Gianluca Tenore, Umberto Romeo, Guido Migliau
Adhesive dentistry has undergone great progress in the last decades. In light of minimal-invasive dentistry, this new approach promotes a more conservative cavity design, which relies on the effectiveness of current enamel-dentine adhesives. Adhesive dentistry began in 1955 by Buonocore on the benefits of acid etching. With changing technologies, dental adhesives have evolved from no-etch to total-etch (4(th) and 5(th) generation) to self-etch (6(th), 7(th) and 8(th) generation) systems. Currently, bonding to dental substrates is based on three different strategies: 1) etch-and-rinse, 2) self-etch and 3) resin-modified glass-ionomer approach as possessing the unique properties of self-adherence to the tooth tissue...
January 2017: Annali di Stomatologia
https://www.readbyqxmd.com/read/28736531/assessment-of-hepatocellular-carcinoma-metastasis-glycobiomarkers-using-advanced-quantitative-n-glycoproteome-analysis
#15
Tianhua Liu, Shuxin Shang, Wei Li, Xue Qin, Lu Sun, Shu Zhang, Yinkun Liu
Hepatocelluar carcinoma (HCC) is one of the most common malignant tumors with high incidence of metastasis. Glycosylation is involved in fundamental molecular and cell biology process occurring in cancer including metastasis formation. In this study, lectin microarray, lectin blotting, lectin affinity chromatography and tandem (18)O stable isotope labeling coupled with liquid chromatography-mass spectrometer (LC-MS) analysis were applied to quantify the changes in N-glycosite occupancy for HCC metastasis serum...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28736431/combined-blockade-of-t-cell-immunoglobulin-and-mucin-domain-3-and-carcinoembryonic-antigen-related-cell-adhesion-molecule-1-results-in-durable-therapeutic-efficacy-in-mice-with-intracranial-gliomas
#16
Jinhu Li, Xiaodong Liu, Yijun Duan, Yueting Liu, Hongqin Wang, Shizhong Lian, Guotao Zhuang, Yimin Fan
BACKGROUND Glioblastoma multiforme (GBM) evades immune surveillance by inducing immunosuppression via receptor-ligand interactions between immune checkpoint molecules. T cell immunoglobulin and mucin domain 3 (Tim-3) is a key checkpoint receptor responsible for exhaustion and dysfunction of T cells and plays a critical role in immunosuppression. Carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) has been recently identified as a heterophilic ligand for Tim-3. MATERIAL AND METHODS We established an intracranial GBM model using C57BL/6 mice and GL261 cells, and treated the mice with single or combined monoclonal antibodies (mAbs) against Tim-3/CEACAM1...
July 24, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28736206/bpag1-in-muscles-structure-and-function-in-skeletal-cardiac-and-smooth-muscle
#17
REVIEW
Masao Horie, Nozomu Yoshioka, Hirohide Takebayashi
BPAG1, also known as Dystonin or BP230, belongs to the plakin family of proteins, which has multiple cytoskeleton-binding domains. Several BPAG1 isoforms are produced by a single BPAG1 genomic locus using different promoters and exons. For example, BPAG1a, BPAG1b, and BPAG1e are predominantly expressed in the nervous system, muscle, and skin, respectively. Among BPAG1 isoforms, BPAG1e is well studied because it was first identified as an autoantigen in patients with bullous pemphigoid, an autoimmune skin disease...
July 20, 2017: Seminars in Cell & Developmental Biology
https://www.readbyqxmd.com/read/28735976/predictive-value-of-procalcitonin-for-intestinal-ischemia-and-or-necrosis-in-pediatric-patients-with-adhesive-small-bowel-obstruction-asbo
#18
Eduardo Bracho-Blanchet, Alfredo Dominguez-Muñoz, Emilio Fernandez-Portilla, Cristian Zalles-Vidal, Roberto Davila-Perez
PURPOSE: Evaluate serum procalcitonin (PCT) level as a predictor of intestinal ischemia or necrosis (IN) in patients with postoperative adhesive small bowel obstruction (ASBO). METHODS: Prospective cohort of consecutive patients with ASBO. Patients previously treated with antibiotics or septic were excluded. PCT was measured at the diagnosis of ASBO and every 24 h afterwards. MAIN OUTCOME: intestinal ischemia or necrosis (IN). RESULTS: Fifty-nine patients were included, 12 of whom were excluded; 47 patients remained in the study; male-to-female ratio = 1...
July 13, 2017: Journal of Pediatric Surgery
https://www.readbyqxmd.com/read/28735704/effect-of-remote-cesarean-delivery-on-complications-during-hysterectomy-a-cohort-study
#19
Susanne Hesselman, Ulf Högberg, Maria Jonsson
BACKGROUND: Cesarean section is frequently performed worldwide, and follow-up studies reporting complications at subsequent surgery are warranted. OBJECTIVES: The aim of the study was to investigate the association between a previous abdominal delivery and complications during a subsequent hysterectomy, and to estimate the fraction of complications driven by the presence of adhesions. STUDY DESIGN: This was a longitudinal population based register study of 25354 women undergoing a benign hysterectomy at 46 hospital units in Sweden 2000-2014...
July 20, 2017: American Journal of Obstetrics and Gynecology
https://www.readbyqxmd.com/read/28735667/immunohistochemical-expression-of-mcam-cd146-in-canine-melanoma
#20
S Abou Asa
MCAM/CD146 (melanoma cell adhesion molecule/CD146) is a transmembrane immunoglobulin superfamily cell adhesion molecule involved in transendothelial migration and signal transduction. It is expressed in melanoma, squamous cell carcinoma, prostatic, ovarian, cervical and endometrial cancers and promotes tumour growth, angiogenesis and metastasis. Melanoma is the most common malignant oral tumour of dogs and also arises in the skin, nail bed and footpad. The aim of this study was to investigate the immunohistochemical expression of MCAM/CD146 in 51 canine melanomas, including oral, cutaneous and ocular tumours...
July 2017: Journal of Comparative Pathology
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