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https://www.readbyqxmd.com/read/28092857/universal-efavirenz-determination-in-transport-study-rat-placenta-perfusion-and-placenta-lysate-by-hplc-uv
#1
Lucie Zelena, Josef Reznicek, Martina Ceckova, Hana Sklenarova
Efavirenz is an antiretroviral drug used in the treatment of HIV-positive patients. A simple, fast and sensitive high-performance liquid chromatography (HPLC) method was developed in order to determine efavirenz in three types of samples provided from pharmacokinetic studies. The analysis took 5min and was performed using a C18 analytical column (Discovery HS C18, 150×4.6mm, particle size of 5μm) in isocratic mode with a mobile phase containing acetonitrile and water (65:35, v/v), a flow rate of 1.6mLmin(-1), a sample volume of 10μL and UV detection at 245nm...
January 11, 2017: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/28092699/infection-of-human-intestinal-epithelial-cells-by-invasive-bacteria-activates-nf-%C3%AE%C2%BAb-and-increases-icam-1-expression-through-nod1
#2
Woon Geon Shin, Bum Joon Park, Sung Joong Lee, Jae Gyu Kim
Background/Aims: Nucleotide-binding oligomerization domain 1 (NOD1) is required for primary intestinal epithelial cells (IECs) to respond to natural mucopeptides secreted by gram-negative bacteria. Infection of human IECs with invasive bacteria up-regulates intercellular adhesion molecule-1 (ICAM-1) expression. However, the role of NOD family members in host defense has been largely unknown. The aim of this study was to determine whether there is a functional role for NOD1 in the up-regulation of ICAM-1 expression in invasive bacteria-infected IECs...
January 16, 2017: Korean Journal of Internal Medicine
https://www.readbyqxmd.com/read/28092671/pde4d-promotes-fak-mediated-cell-invasion-in-braf-mutated-melanoma
#3
J Delyon, A Servy, F Laugier, J André, N Ortonne, M Battistella, S Mourah, A Bensussan, C Lebbé, N Dumaz
The cyclic AMP (cAMP) signaling pathway is critical in melanocyte biology for regulating differentiation. It is downregulated by phosphodiesterase (PDE) enzymes, which degrade cAMP itself. In melanoma evidence suggests that inhibition of the cAMP pathway by PDE type 4 (PDE4) favors tumor progression. For example, in melanomas harboring RAS mutations, the overexpression of PDE4 is crucial for MAPK pathway activation and proliferation induced by oncogenic RAS. Here we showed that PDE4D is overexpressed in BRAF-mutated melanoma cell lines, constitutively disrupting the cAMP pathway activation...
January 16, 2017: Oncogene
https://www.readbyqxmd.com/read/28092639/lithographic-patterning-of-antibodies-by-direct-lift-off-and-improved-surface-adhesion
#4
Sankalp Verma, Vivek Verma
The inherent property of antibodies binding to their antigen with high specificity makes them a strong candidate for sensing and detection applications. Microscale patterning of antibodies is desired for the miniaturization of sensors and fundamental cell biology studies. However, existing methodologies to pattern antibodies at the microscale are multi-step. In this work, we demonstrate microscale patterning of antibodies on a glass coverslip in a single step photolithography process. The microscale features of the photoresist were generated on the coverslip using photolithography, and the antibody solution was incubated...
January 16, 2017: Biofabrication
https://www.readbyqxmd.com/read/28092460/a-three-dimensional-lymphatic-endothelial-cell-tube-formation-assay-to-identify-novel-kinases-involved-in-lymphatic-vessel-remodeling
#5
T Jessica Gambino, Steven P Williams, Carol Caesar, Daniel Resnick, Cameron J Nowell, Rae H Farnsworth, Marc G Achen, Steven A Stacker, Tara Karnezis
The lymphatic system is a series of vessels that transport cells and excess fluid from tissues to the blood vascular system. Normally quiescent, the lymphatics can grow or remodel in response to developmental, immunological, or cells pathological stimuli. Lymphatic vessels comprise lymphatic endothelial cells (LECs) that can respond to external growth factors by undergoing proliferation, migration, adhesion, and tube and lumen formation into new vessel structures, a process known as lymphangiogenesis. To understand the key gene and signaling pathways necessary for lymphangiogenesis and lymphatic vessel remodeling, we have developed a three-dimensional LEC tube formation assay to explore the role of kinase signaling in these processes...
January 2017: Assay and Drug Development Technologies
https://www.readbyqxmd.com/read/28092438/biofunctionalization-of-nerve-interface-via-biocompatible-polymer-roughened-pt-black-on-cuff-electrode-for-chronic-recording
#6
Yi Jae Lee, Han-Jun Kim, Ji Yoon Kang, Sun Hee Do, Soo Hyun Lee
Peripheral nerve cuff electrodes with roughened Pt black (BPt) are coated with polyethylene glycol (PEG) and Nafion (NF). Although the influence of coated PEG and Nafion on roughened BPt on the electrical properties is weak, the cuff electrode with BPt/PEG and BPt/Nafion exhibits some very important properties. For example, it markedly decreases interfacial impedance, increases charge storage capacity (CSC) due to retaining the BPt surface structure, good stability without exfoliation in repetitive cyclic voltammetry scanning because it is protected by PEG or Nafion coating...
January 16, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28092391/correlation-of-focal-adhesion-assembly-and-disassembly-with-cell-migration-on-nanotopography
#7
Elena I Liang, Emma J Mah, Albert F Yee, Michelle A Digman
Selective cell adhesion is desirable to control cell growth and migration on biomedical implants. Mesenchymal cell migration is regulated through focal adhesions (FAs) and can be modulated by their microenvironment, including changes in surface topography. We use the Number and Molecular Brightness (N&B) imaging analysis to provide a unique perspective on FA assembly and disassembly. This imaging analysis generates a map of real-time fluctuations of protein monomers, dimers, and higher order aggregates of FA proteins, such as paxillin during assembly and disassembly...
January 16, 2017: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/28092337/anatomical-basis-and-clinical-application-of-synovial-flaps-in-the-wrist-and-distal-forearm
#8
David L Colen, Jiun-Ting Yeh, Lawrence B Colen
Neuropathic symptoms after median nerve repair at the wrist or secondary to refractory carpal tunnel syndrome may become debilitating. These symptoms develop due to perineural adhesions, intraneural fibrosis and fixation of the nerve to the transverse carpal ligament after surgery and often require neurolysis. Interposition of vascularized soft tissue over the median nerve at the time of neurolysis prevents recurrence of such adhesions. The synovial flap, fashioned from the synovial lining of the flexor tendon sheath, is an ideal tissue for this purpose...
January 12, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28092265/the-fibronectin-synergy-site-re-enforces-cell-adhesion-and-mediates-a-crosstalk-between-integrin-classes
#9
Maria Benito-Jardón, Sarah Klapproth, Irene Gimeno-LLuch, Tobias Petzold, Mitasha Bharadwaj, Daniel J Müller, Gabriele Zuchtriegel, Christoph A Reichel, Mercedes Costell
Fibronectin (FN), a major extracellular matrix component, enables integrin-mediated cell adhesion via binding of α5β1, αIIbβ3 and αv-class integrins to an RGD-motif. An additional linkage for α5 and αIIb is the synergy site located in close proximity to the RGD motif. We report that mice with a dysfunctional FN-synergy motif (FNsyn/syn) suffer from surprisingly mild platelet adhesion and bleeding defects due to delayed thrombus formation after vessel injury. Additional loss of β3 integrins dramatically aggravates the bleedings and severely compromises smooth muscle cell coverage of the vasculature leading to embryonic lethality...
January 16, 2017: ELife
https://www.readbyqxmd.com/read/28092156/inhibition-of-cronobacter-sakazakii-adhesion-to-caco-2-cells-by-commercial-dairy-powders-and-raw-buttermilk
#10
Daniel Ripollés, Saidou Harouna, José Antonio Parrón, Irene Arenales, Miguel Calvo, María Dolores Pérez, Lourdes Sánchez
Cronobacter sakazakii is a foodborne pathogen that has been associated with severe infections mainly in neonates. The binding of this bacterium to host cell surfaces represents the first step in the pathogenesis of disease. An ELISA-based assay has been developed using a polyclonal antiserum against C. sakazakii to determine its adhesion to Caco-2 cells. The antiserum used recognised many of the outer membrane proteins of C. sakazakii. A positive correlation was found between the absorbance values obtained by ELISA and the number of bacteria adhered to cells determined by plate counting...
January 16, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28092153/removable-large-area-ultra-smooth-silver-nanowire-transparent-composite-electrode
#11
Yunxia Jin, Kaiqing Wang, Yuanrong Cheng, Qibing Pei, Yuxi Xu, Fei Xiao
In this work, a composite silver nanowire (AgNW) transparent electrode that is large-area ultra-smooth without conductivity or transmittance scarifice, removable but with good resistance to both water and organic solvent is reported. Via a simple low-temperature solution process without complicated transfer steps or additional pressure pressing, a new kind of AgNWs composite with biocompatible and patternable chitosan polymer complex demonstrates a quite low RMS (root mean square) roughness ~7 nm at a largest reported scan size of 50 μm*50 μm, which is among the best flat surface...
January 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28091946/self-catalyzed-growth-of-inas-nanowires-on-inp-substrate
#12
Bang Li, Xin Yan, Xia Zhang, Xiaomin Ren
We report on the self-catalyzed growth of InAs nanowires on InP substrate by metal-organic chemical vapor deposition. At a moderate V/III ratio, tapered nanowires are obtained, suggesting a strong surface diffusion effect. Dense twin faults are observed perpendicular to the nanowire growth direction due to the fluctuation of In atoms in the droplet originating from the surface diffusion effect. At a lower V/III ratio, the nanowires exhibit kinking, which is associated with a high adhesion due to a large sticking coefficient of TMIn...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28091914/altered-liver-proteoglycan-glycosaminoglycan-structure-as-a-manifestation-of-extracellular-matrix-remodeling-upon-bcg-induced-granulomatosis-in-mice
#13
L B Kim, V A Shkurupy, A N Putyatina
Experimental BCG-induced granulomatosis in mice was used to study changes in the dynamics of individual liver proteoglycan components reflecting phasic extracellular matrix remodeling, determined by the host-parasite interaction and associated with granuloma development. In the early BCG-granulomatosis period, the increase in individual proteoglycan components promotes granuloma formation, providing conditions for mycobacteria adhesion to host cells, migration of phagocytic cells from circulation, and cell-cell interaction leading to granuloma development and fibrosis...
January 14, 2017: Bulletin of Experimental Biology and Medicine
https://www.readbyqxmd.com/read/28091660/universal-wetting-transition-of-an-evaporating-water-droplet-on-hydrophobic-micro-and-nano-structures
#14
Adrien Bussonnière, Masoud B Bigdeli, Di-Yen Chueh, Qingxia Liu, Peilin Chen, Peichun Amy Tsai
Water-repellent, rough surfaces have a remarkable and beneficial wetting property: when a water droplet comes in contact with a small fraction of the solid, both liquid-solid adhesion and hydrodynamic drag are reduced. As a prominent example from nature, the lotus leaf-comprised of a wax-like material with micro- and nano-scaled roughness-has recently inspired numerous syntheses of superhydrophobic substrates. Due to the diverse applications of superhydrophobicity, much research has been devoted to the fabrication and investigations of hydrophobic micro-structures using established micro-fabrication techniques...
January 16, 2017: Soft Matter
https://www.readbyqxmd.com/read/28091531/tnf-%C3%AE-regulates-the-proteolytic-degradation-of-st6gal-1-and-endothelial-cell-cell-junctions-through-upregulating-expression-of-bace1
#15
Xiao Deng, Jun Zhang, Yan Liu, Linmu Chen, Chao Yu
Endothelial dysfunction and monocyte adhesion to vascular endothelial cells are two critical steps in atherosclerosis development, and emerging evidence suggests that protein sialylation is involved in these processes. However, the mechanism underlying this phenomenon remains incompletely elucidated. In this study, we demonstrated that treatment with the proinflammatory cytokine TNF-α disrupted vascular endothelial cell-cell tight junctions and promoted monocyte endothelial cell adhesion. Western blotting and Sambucus nigra lectin (SNA) blotting analyses revealed that TNF-α treatment decreased α-2, 6-sialic acid transferase 1 (ST6Gal-I) levels and downregulated VE-Cadherin α-2, 6 sialylation...
January 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28091433/clinical-evaluation-of-microhybrid-composites-in-noncarious-cervical-lesions-24-month-results
#16
D Tuncer, C Çelik, K Yamanel, N Arhun
OBJECTIVE: To evaluate the clinical performance of two different microhybrid resin composites in noncarious cervical lesions (NCCLs) after 24 months. SUBJECTS AND METHODS: Ninety-seven NCCLs were restored with either TPH Spectrum (n = 48) or Filtek Z250 (n = 49) using an etch-and-rinse adhesive in 20 patients. The restorations were clinically evaluated using modified United States Public Health Service criteria for retention, color match, marginal discoloration, marginal adaptation, surface texture, anatomic form, postoperative sensitivity, and secondary caries...
February 2017: Nigerian Journal of Clinical Practice
https://www.readbyqxmd.com/read/28091411/protective-effects-of-calpain-inhibition-on-neurovascular-unit-injury-through-downregulating-nuclear-factor-%C3%AE%C2%BAb-related-inflammation-during-traumatic-brain-injury-in-mice
#17
Xiao-Gang Tao, Jing-Hua Shi, Shu-Yu Hao, Xue-Tao Chen, Bai-Yun Liu
BACKGROUND: In addition to neurons, all components of the neurovascular unit (NVU), such as glial, endothelial, and basal membranes, are destroyed during traumatic brain injury (TBI). Previous studies have shown that excessive stimulation of calpain is crucial for cerebral injury after traumatic insult. The objective of this study was to investigate whether calpain activation participated in NVU disruption and edema formation in a mouse model of controlled cortical impact (CCI). METHODS: One hundred and eight mice were divided into three groups: the sham group, the control group, and the MDL28170 group...
2017: Chinese Medical Journal
https://www.readbyqxmd.com/read/28090771/differential-adhesive-bioactive-properties-of-polymeric-surface-coated-with-graphene-oxide-thin-film
#18
Sudhin Thampi, A Maya Nandkumar, Vignesh Muthuvijayan, Ramesh Parameswaran
Surface engineering of implantable devices involving polymeric biomaterials has become an essential aspect for medical implants. Surface enhancement technique can provide an array of unique surface properties that improves its biocompatibility and functionality as an implant. Polyurethane based implants that have found extensively acclaimed usage as an implant in biomedical applications, especially in the area of cardiovascular devices still lacks any mechanism to ward off bacterial or platelet adhesion. To bring out such a defense mechanism we are proposing a surface modification technique...
January 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28090711/topical-application-of-nitrosonifedipine-a-novel-radical-scavenger-ameliorates-ischemic-skin-flap-necrosis-in-a-mouse-model
#19
Yutaka Fukunaga, Yuki Izawa-Ishizawa, Yuya Horinouchi, Eriko Sairyo, Yasumasa Ikeda, Keisuke Ishizawa, Koichiro Tsuchiya, Yoshiro Abe, Ichiro Hashimoto, Toshiaki Tamaki
Ischemic skin flap necrosis can occur in random pattern flaps. An excess amount of reactive oxygen species is generated and causes necrosis in the ischemic tissue. Nitrosonifedipine (NO-NIF) has been demonstrated to possess potent radical scavenging ability. However, there has been no study on the effects of NO-NIF on ischemic skin flap necrosis. Therefore, we evaluated the potential of NO-NIF in ameliorating ischemic skin flap necrosis in a mouse model. A random pattern skin flap (1.0 × 3.0 cm) was elevated on the dorsum of C57BL/6 mice...
January 16, 2017: Wound Repair and Regeneration
https://www.readbyqxmd.com/read/28090705/osteogenic-differentiation-potential-of-adipose-derived-stem-cells-from-ovariectomized-mice
#20
Lei Wang, Chenglong Huang, Qing Li, Xiaomei Xu, Lin Liu, Kui Huang, Xiaoxiao Cai, Jingang Xiao
OBJECTIVES: Osteoporosis (OP) is a systemic disease caused by imbalance between bone resorption and bone formation, commonly resulting from post-menopausal oestrogen deficiency. Although osteogenic differentiation potential of adipose-derived stem cells (ASCs) has been demonstrated, the effect of OP on osteogenic differentiation of ASCs remains unclear. Here, our work has been designed to compare proliferative capacity and osteogenic differentiation ability of ASCs obtained from osteoporotic mice and normal control mice...
January 16, 2017: Cell Proliferation
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