Rachel Liuqing Shi, Michael A Dillon, Philip D Compton, William S Sawyer, John R Thorup, Mandy Kwong, Pamela Chan, Cecilia P C Chiu, Ran Li, Rajbharan Yadav, Genee Y Lee, Joshua G Gober, Zhiyu Li, Adel M ElSohly, Ayse Meric Ovacik, James T Koerber, Christoph Spiess, Jonathan L Josephs, John C Tran
Intact protein mass spectrometry (MS) coupled with liquid chromatography was applied to characterize the pharmacokinetics and stability profiles of therapeutic proteins. However, limitations from chromatography, including throughput and carryover, result in challenges with handling large sample numbers. Here, we combined intact protein MS with multiple front-end separations, including affinity capture, SampleStream, and high-field asymmetric waveform ion mobility spectrometry (FAIMS), to perform high-throughput and specific mass measurements of a multivalent antibody with one antigen-binding fragment (Fab) fused to an immunoglobulin G1 (IgG1) antibody...
November 13, 2023: Analytical Chemistry