keyword
MENU ▼
Read by QxMD icon Read
search

Field AND asymmetric AND ion AND mobility AND spectrometry

keyword
https://www.readbyqxmd.com/read/30007914/a-novel-differential-ion-mobility-device-expands-the-depth-of-proteome-coverage-and-the-sensitivity-of-multiplex-proteomic-measurements
#1
Sibylle Pfammatter, Eric Bonneil, Francis P McManus, Satendra Prasad, Derek J Bailey, Michael Belford, Jean-Jacques Dunyach, Pierre Thibault
The depth of proteomic analyses is often limited by the overwhelming proportion of confounding background ions that compromise the identification and quantification of low abundance peptides. To alleviate these limitations, we present a new high field asymmetric waveform ion mobility spectrometry (FAIMS) interface that can be coupled to the Orbitrap Tribrid mass spectrometers. The interface provides several advantages over previous generations of FAIMS devices including ease of operation, robustness, and high ion transmission...
July 14, 2018: Molecular & Cellular Proteomics: MCP
https://www.readbyqxmd.com/read/29985339/analyzing-the-photo-oxidation-of-2-propanol-at-indoor-air-level-concentrations-using-field-asymmetric-ion-mobility-spectrometry
#2
Christopher P Ireland, Michael Coto, Lauren Brown, Russell Paris, Caterina Ducati
We demonstrate a versatile protocol to be used for determining the effectiveness of photocatalysts in degrading indoor air concentration (ppb) volatile organic carbons (VOCs), illustrating this with a titanium dioxide based catalyst, and the VOC 2-propanol. The protocol takes advantage of field asymmetric ion mobility spectroscopy (FAIMS), an analysis tool that is capable of continuously identifying and monitoring the concentration of VOCs such as 2-propanol and acetone at the ppb level. The continuous nature of FAIMS allows detailed kinetic analysis, and long-term reactions, offering a significant advantage over gas chromatography, a batch process traditionally used in air purification characterization...
June 14, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29969236/rapid-and-comprehensive-single-shot-proteomics-with-lc-faims-ms-ms
#3
Alexander S Hebert, Satendra Prasad, Michael W Belford, Derek J Bailey, Graeme C McAlister, Susan E Abbatiello, Romain Huguet, Eloy R Wouters, Jean-Jacques Dunyach, Dain R Brademan, Michael S Westphall, Joshua J Coon
Liquid chromatography pre-fractionation is often implemented to increase proteomic coverage; however, while effective, this approach is laborious, requires considerable sample amount, and can be cumbersome. We describe how interfacing a recently described High Field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) device between a nanoESI-emitter and an Orbitrap hybrid mass spectrometer enables the collection of single shot proteomic data with comparable depth to conventional two-dimensional LC approaches...
July 3, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29931245/late-onset-sepsis-in-preterm-infants-can-be-detected-preclinically-by-fecal-volatile-organic-compound-analysis-a-prospective-multicenter-cohort-study
#4
Daniel J C Berkhout, Britt J van Keulen, Hendrik J Niemarkt, Jet R Bessem, Willem P de Boode, Veerle Cossey, Neil Hoogenes, Christiaan V Hulzebos, Ellen Klaver, Peter Andriessen, Anton H van Kaam, Boris W Kramer, Richard A van Lingen, Aaron Schouten, Johannes B van Goudoever, Daniel C Vijlbrief, Mirjam M van Weissenbruch, Alfian N Wicaksono, James A Covington, Marc A Benninga, Nanne K H de Boer, Tim G J de Meij
Background: The intestinal microbiota has increasingly been considered to play a role in the etiology of late-onset sepsis (LOS). We hypothesize that early alterations in fecal volatile organic compounds (VOCs), reflecting intestinal microbiota composition and function, allow for discrimination between infants developing LOS and controls in a preclinical stage. Methods: In 9 neonatal intensive care units in the Netherlands and Belgium, fecal samples of preterm infants born at a gestational age ≤30 weeks were collected daily, up to the postnatal age of 28 days...
June 21, 2018: Clinical Infectious Diseases: An Official Publication of the Infectious Diseases Society of America
https://www.readbyqxmd.com/read/29909416/non-invasive-detection-of-anastomotic-leakage-following-esophageal-and-pancreatic-surgery-by-urinary-analysis
#5
Victor D Plat, Nora van Gaal, James A Covington, Matthew Neal, Tim G J de Meij, Donald L van der Peet, Babs Zonderhuis, Geert Kazemier, Nanne K H de Boer, Freek Daams
BACKGROUND: Esophagectomy or pancreaticoduodenectomy is the standard surgical approach for patients with tumors of the esophagus or pancreatic head. Postoperative mortality is strongly correlated with the occurrence of anastomotic leakage (AL). Delay in diagnosis leads to delay in treatment, which ratifies the need for development of novel and accurate non-invasive diagnostic tests for detection of AL. Urinary volatile organic compounds (VOCs) reflect the metabolic status of an individual, which is associated with a systemic immunological response...
June 15, 2018: Digestive Surgery
https://www.readbyqxmd.com/read/29860824/optimized-sampling-conditions-for-fecal-volatile-organic-compound-analysis-by-means-of-field-asymmetric-ion-mobility-spectrometry
#6
Sofie Bosch, Sofia El Manouni El Hassani, James A Covington, Alfian N Wicaksono, Marije K Bomers, Marc A Benninga, Chris J J Mulder, Nanne K H de Boer, Tim G J de Meij
Fecal volatile organic compounds (VOCs) are increasingly considered to be potential noninvasive, diagnostic biomarkers for various gastrointestinal diseases. Knowledge of the influence of sampling conditions on VOC outcomes is limited. We aimed to evaluate the effects of sampling conditions on fecal VOC profiles and to assess under which conditions an optimal diagnostic accuracy in the discrimination between pediatric inflammatory bowel disease (IBD) and controls could be obtained. Fecal samples from de novo treatment-naïve pediatric IBD patients and healthy controls (HC) were used to assess the effects of sampling conditions compared to the standard operating procedure (reference standard), defined as 500 mg of sample mass diluted with 10 mL tap water, using field asymmetric ion mobility spectrometry (FAIMS)...
July 3, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29800516/desorption-electrospray-ionization-mass-spectrometry-imaging-of-proteins-directly-from-biological-tissue-sections
#7
Kyana Y Garza, Clara L Feider, Dustin R Klein, Jake A Rosenberg, Jennifer S Brodbelt, Livia S Eberlin
Analysis of large biomolecules including proteins has proven challenging using ambient ionization mass spectrometry imaging techniques. Here, we have successfully optimized desorption electrospray ionization mass spectrometry (DESI-MS) to detect intact proteins directly from tissue sections and further integrated DESI-MS to a high field asymmetric waveform ion mobility (FAIMS) device for protein imaging. Optimized DESI-FAIMS-MS parameters were used to image mouse kidney, mouse brain, and human ovarian and breast tissue samples, allowing detection of 11, 16, 14, and 16 proteoforms, respectively...
July 3, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29788410/differentiation-between-pediatric-irritable-bowel-syndrome-and-inflammatory-bowel-disease-based-on-fecal-scent-proof-of-principle-study
#8
Sofie Bosch, Nora van Gaal, Roy P Zuurbier, James A Covington, Alfian N Wicaksono, Maarten H Biezeveld, Marc A Benninga, Chris J Mulder, Nanne K H de Boer, Tim G J de Meij
Background: The diagnostic work-up of pediatric irritable bowel syndrome (IBS) and functional abdominal pain-not otherwise specified (FAP-NOS) commonly includes invasive tests for discrimination from inflammatory bowel disease (IBD). As this carries a high burden on patients, an ongoing need exists for development of noninvasive diagnostic biomarkers for IBS and FAP-NOS. Several studies have shown microbiota alterations in IBS/FAP, which are considered to be reflected by fecal volatile organic compounds (VOCs)...
May 18, 2018: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/29707944/high-field-asymmetric-waveform-ion-mobility-spectrometry-in-nontargeted-bottom-up-proteomics-of-dried-blood-spots
#9
Cecilie Rosting, Jinglei Yu, Helen J Cooper
Despite the great potential of dried blood spots (DBS) as a source of endogenous proteins for biomarker discovery, the literature relating to nontargeted bottom-up proteomics of DBS is sparse, primarily due to the inherent complexity and very high dynamic range associated with these samples. Here, we present proof-of-concept results in which we have coupled high field asymmetric waveform ion mobility spectrometry (FAIMS) with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for nontargeted bottom-up proteomics of DBS with the aim of addressing these challenges...
June 1, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29623662/gas-phase-enrichment-of-multiply-charged-peptide-ions-by-differential-ion-mobility-extend-the-comprehensiveness-of-sumo-proteome-analyses
#10
Sibylle Pfammatter, Eric Bonneil, Francis P McManus, Pierre Thibault
The small ubiquitin-like modifier (SUMO) is a member of the family of ubiquitin-like modifiers (UBLs) and is involved in important cellular processes, including DNA damage response, meiosis and cellular trafficking. The large-scale identification of SUMO peptides in a site-specific manner is challenging not only because of the low abundance and dynamic nature of this modification, but also due to the branched structure of the corresponding peptides that further complicate their identification using conventional search engines...
June 2018: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/29607564/ambient-ionisation-mass-spectrometry-for-in-situ-analysis-of-intact-proteins
#11
Klaudia I Kocurek, Rian L Griffiths, Helen J Cooper
Ambient surface mass spectrometry is an emerging field which shows great promise for the analysis of biomolecules directly from their biological substrate. In this article, we describe ambient ionisation mass spectrometry techniques for the in situ analysis of intact proteins. As a broad approach, the analysis of intact proteins offers unique advantages for the determination of primary sequence variations and posttranslational modifications, as well as interrogation of tertiary and quaternary structure and protein-protein/ligand interactions...
July 2018: Journal of Mass Spectrometry: JMS
https://www.readbyqxmd.com/read/29412650/can-mutational-analysis-be-used-to-assist-structure-determination-of-peptides
#12
Liudmila Voronina, Valeriu Scutelnic, Chiara Masellis, Thomas R Rizzo
Mutational analysis is widely used to study the relationship between sequence and structure of proteins and peptides. It is often assumed that substituting a proline with another amino acid "locks" the peptide bond in the trans conformation, allowing only a subset of the initial molecular geometries to be observed. To test this assumption, we assess the result of substituting two prolines in the bradykinin sequence with alanine using field-asymmetric ion mobility spectrometry combined with cryogenic ion spectroscopy in the gas phase...
February 21, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29380926/integration-of-paper-spray-ionization-high-field-asymmetric-waveform-ion-mobility-spectrometry-for-forensic-applications
#13
Chia-Wei Tsai, Christopher A Tipple, Richard A Yost
RATIONALE: Paper spray ionization (PSI) is an attractive ambient ionization source for mass spectrometry (MS) since it allows the combination of surface sampling and ionization. The minimal sample preparation inherent in this approach greatly reduces the time needed for analysis. However, the ions generated from interfering compounds in the sample and the paper substrate may interfere with the analyte ions. Therefore, the integration of PSI with high-field asymmetric ion mobility spectrometry (FAIMS) is of significant interest since it should reduce the background ions entering the mass analyzer without complicating the analysis or increasing analysis time...
April 15, 2018: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29335669/portable-faims-applications-and-future-perspectives
#14
Michael T Costanzo, Jared J Boock, Robin H J Kemperman, Michael S Wei, Christopher R Beekman, Richard A Yost
Miniaturized mass spectrometry (MMS) is optimal for a wide variety of applications that benefit from field-portable instrumentation. Like MMS, field asymmetric ion mobility spectrometry (FAIMS) has proven capable of providing in situ analysis, allowing researchers to bring the lab to the sample. FAIMS compliments MMS very well, but has the added benefit of operating at atmospheric pressure, unlike MS. This distinct advantage makes FAIMS uniquely suited for portability. Since its inception, FAIMS has been envisioned as a field-portable device, as it affords less expense and greater simplicity than many similar methods...
November 2017: International Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/29183198/evaluation-of-calculated-negative-mode-ion-mobilities-of-small-molecules-in-air
#15
Frank Gunzer
Ion mobility spectrometry is a well-known technique employed for the detection and analysis of gaseous substances. In pharmaceutical applications, it is furthermore used for structural analysis of compounds, especially in combination with mass spectrometry. In this field, the comparison of calculated collision cross sections and ion mobilities of theoretic model compounds with experimental values measured with ion mobility spectrometers helps to determine the compound's structure. For positive mode ion mobility spectrometry, the calculated mobilities using the Trajectory Method show in general a deviation of 10% or less from experimental values...
December 2017: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/29179535/differential-ion-mobility-separations-in-the-low-pressure-regime
#16
Alexandre A Shvartsburg, Anisha Haris, Roch Andrzejewski, Andrew Entwistle, Roger Giles
Ion mobility spectrometry (IMS) in conjunction with mass spectrometry (MS) has emerged as a powerful platform for biological and environmental analyses. An inherent advantage of differential or field asymmetric waveform IMS (FAIMS) based on the derivative of mobility vs electric field over linear IMS based on absolute mobility is much greater orthogonality to MS. Effective coupling of linear IMS to MS and diverse IMS/MS arrangements and modalities impossible at ambient buffer gas pressure were enabled at much reduced pressures...
January 2, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/29169249/an-intelligent-detection-method-for-high-field-asymmetric-waveform-ion-mobility-spectrometry
#17
Yue Li, Jianwen Yu, Zhiming Ruan, Chilai Chen, Ran Chen, Han Wang, Youjiang Liu, Xiaozhi Wang, Shan Li
In conventional high-field asymmetric waveform ion mobility spectrometry signal acquisition, multi-cycle detection is time consuming and limits somewhat the technique's scope for rapid field detection. In this study, a novel intelligent detection approach has been developed in which a threshold was set on the relative error of α parameters, which can eliminate unnecessary time spent on detection. In this method, two full-spectrum scans were made in advance to obtain the estimated compensation voltage at different dispersion voltages, resulting in a narrowing down of the whole scan area to just the peak area(s) of interest...
April 2018: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/29028396/laser-stimulated-desorption-of-organic-molecules-from-surfaces-as-a-method-of-increasing-the-efficiency-of-ion-mobility-spectrometry-analysis
#18
Artem E Akmalov, Alexander A Chistyakov, Gennadii E Kotkovskii
Application of laser-induced desorption was investigated as a method of increasing the efficiency of gas phase analyzers on principles of field asymmetric ion mobility spectrometry. Mass spectrometric data of investigations of laser desorption of pentaerythritoltetranitrate molecules and cyclotetramethylenetetranitramine molecules from quartz substrate under vacuum were obtained. Laser sources a Nd3+ :YAG with nanosecond pulse duration (λ = 532 nm) and a continuous wave diode laser (λ = 440 nm) were used...
August 2017: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/28964226/performance-enhancement-of-high-field-asymmetric-waveform-ion-mobility-spectrometry-by-applying-differential-rf-driven-operation-mode
#19
Yue Zeng, Fei Tang, Yadong Zhai, Xiaohao Wang
The traditional operation mode of high-field Asymmetric Waveform Ion Mobility Spectrometry (FAIMS) uses a one-way radio frequency (RF) voltage input as the dispersion voltage. This requires a high voltage input and limits power consumption reduction and miniaturization of instruments. With higher dispersion voltages or larger compensation voltages, there also exist problems such as low signal intensity or the fact that the dispersion voltage is no longer much larger than the compensation voltage. In this paper, a differential-RF-driven operation mode of FAIMS is proposed...
September 2017: Review of Scientific Instruments
https://www.readbyqxmd.com/read/28613840/rapid-analysis-of-anabolic-steroid-metabolites-in-urine-by-combining-field-asymmetric-waveform-ion-mobility-spectrometry-with-liquid-chromatography-and-mass-spectrometry
#20
Kayleigh L Arthur, Matthew A Turner, Alan D Brailsford, Andrew T Kicman, David A Cowan, James C Reynolds, Colin S Creaser
The combination of field asymmetric waveform ion mobility spectrometry with liquid chromatography-mass spectrometry (LC-FAIMS-MS) has been developed for the analysis of glucuronide and sulfate metabolites of seven anabolic-androgenic steroids in urine. Separation by FAIMS-MS was investigated in positive ion mode for selected cationic adducts (H(+), NH4(+), Na(+), K(+), and Cs(+)). LC-FAIMS-MS analysis of the doubly sodiated adducts ([M + 2Na - H](+)) of isobaric and coeluting steroid metabolites allowed their rapid (8 min) qualitative and quantitative determination in spiked urine using hydrophilic interaction liquid chromatography prior to FAIMS-MS separation, with discrimination >95% achieved between the steroids investigated...
June 30, 2017: Analytical Chemistry
keyword
keyword
168398
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"