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E Wenzler, M Lee, T J Wu, K A Meyer, R K Shields, M H Nguyen, C J Clancy, R M Humphries, A T Harrington
Objectives: To ensure the accuracy of susceptibility testing methods for ceftazidime/avibactam. Methods: The performances of the Etest (bioMérieux), 30/20 μg disc (Hardy diagnostics) and 10/4 μg disc (Mast Group) were evaluated against the reference broth microdilution (BMD) method for 102 clinically relevant Gram-negative organisms: 69 ceftazidime- and meropenem-resistant Klebsiella pneumoniae and 33 MDR non-K. pneumoniae. Essential and categorical agreement along with major and very major error rates were determined according to CLSI guidelines...
December 10, 2018: Journal of Antimicrobial Chemotherapy
Nydia A Castillo-Martínez, Rosa R Mouriño-Pérez, José M Cornejo-Bravo, Luis A Gaitán-Cepeda
BACKGROUND: Factors associated with candidiasis and colonization in HIV-positive children and adolescents in developing countries are not well understood. AIM: To identify the factors associated with oral Candida colonization and candidiasis in institutionalized HIV-positive children and adolescents in Tijuana, México, as well as the response of the isolates to antifungals. MATERIALS AND METHODS: Sample of the oral mucosa of 30 HIV positive children and adolescents were obtained to isolate and identify Candida species by culture and metabolic profile...
August 2018: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
Valerie Corvalán, Beatrice Hervé, Camila Sanhueza, Nicolás Martínez, María Almonacid, Sebastián De La Fuente
Currently, the use of cefazolin is recommended to determine the susceptibility to first-generation oral cephalosporins in strains of enterobacteria in uncomplicated UTI. We determined susceptibility differences to oral cephalosporins in urinary strains according to cefazolin or cefalotin breakpoints and the correlation of susceptibility between cefazolin and cefadroxil. We studied 52 strains with cefalotin and cefazolin by disk-diffusion and MIC (Kirby-Bauer and Vitek XL) and a subgroup by disk-diffusion for cefadroxil...
2018: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
Caner Yürüyen, Şölen Daldaban Dinçer, Özgür Yanılmaz, Efe Serkan Boz, Sebahat Aksaray
Empiric treatment with broad-spectrum antibiotics exerts condensed pressure in intensive care units (ICUs) for the selection of multidrug-resistant bacteria. Multidrug-resistant gram-negative bacteria became the focus of interest owing to limited treatment options and negative effects on patient survival. Cumulative antibiograms can guide selection of correct empiric treatment, de-escalation treatment according to antibiogram results and development of policies in fight against antibiotic resistance. In this study, we aimed to determine the antibiotic resistance rates of gram-negative bacilli in the intensive care units of the public hospitals in the region where we are connected by using the cumulative antibiogram result and to show the change of resistance over the years and to determine whether there is any difference between the hospitals...
October 2018: Mikrobiyoloji Bülteni
Y Guo, F P Hu, D M Zhu, C Q Wang, A M Wang, H Zhang, C Wang, F Dong, J H Zhen, S P Zhou, Y Zhou, L Zhu, J H Meng
Objective: To investigate the prevalence and resistance changes of carbapenem-resistant Enterobacteriaceae (CRE) strains isolated from children patients of Chinese Bacterial Resistance Surveillance Network (CHINET) from 2005 to 2017. Methods: Antimicrobial susceptibility testing was carried out by disk diffusion method (KB method) and automated systems. Results were analyzed according to the Clinical and Laboratory Standards Institute (CLSI) 2017 edition standards. Results: Among the 4 481 CRE clinical strains, the overall prevalence of CRE in children was 6...
December 2, 2018: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
Barbara A Brown-Elliott, Richard J Wallace
We performed bedaquiline broth microdilution susceptibility using Clinical and Laboratory Standards Institute (CLSI) guidelines on 104 non-duplicate isolates of Mycobacterium abscessus complex [ M. abscessus subsp. abscessus (76), M. abscessus subsp. massiliense (10), M. abscessus subsp. bolletii , (2) and M. abscessus subsp. abscessus /subsp. massiliense hybrid, i.e., M. abscessus subsp. abscessus by rpoB gene and subsp. massiliense by erm (41) gene (16)]. All isolates from patients not known to have been on prior bedaquiline had MIC values ≤0...
December 3, 2018: Antimicrobial Agents and Chemotherapy
Yun Huang, Edward Campbell, Bonita Colbourne, Jeanette Power, Edward Randell
OBJECTIVES: This study investigated the analytical performance of glucose meters while in use for 1.5 years in a hospital setting. The effect of user competency, strip stability and strip lot number variation on the performance of blood glucose meters was investigated. DESIGN AND METHODS: The studies for linearity, accuracy, imprecision, and method comparison were conducted according to Clinical and Laboratory Standards Institute (CLSI) guidelines Point of Care Testing (POCT) 12-A3...
November 30, 2018: Clinical Biochemistry
Andrew J Denisuik, Lauren A Garbutt, Alyssa R Golden, Heather J Adam, Melanie Baxter, Kimberly A Nichol, Philippe Lagacé-Wiens, Andrew J Walkty, James A Karlowsky, Daryl J Hoban, Michael R Mulvey, George G Zhanel
Objectives: To describe the microbiology and antimicrobial resistance patterns of cultured samples acquired from Canadian ICUs. Methods: From 2007 to 2016, tertiary care centres from across Canada submitted 42 938 bacterial/fungal isolates as part of the CANWARD surveillance study. Of these, 8130 (18.9%) were from patients on ICUs. Susceptibility testing guidelines and MIC interpretive criteria were defined by CLSI. Results: Of the 8130 pathogens collected in this study, 58...
November 29, 2018: Journal of Antimicrobial Chemotherapy
Prapti Bora, Priya Datta, Varsha Gupta, Lipika Singhal, Jagdish Chander
PURPOSE: This study has been done to speciate coagulase-negative staphylococci (CoNS) and also study their antibiotic susceptibility pattern isolated from clinical samples. MATERIALS AND METHODS: A total of 120 consecutive CoNS were isolated from various clinical samples such as blood, pus, wound swab, drain fluid, tracheal aspirate, peritoneal fluid, and pleural fluid over a period of 6 months. CoNS were identified by characteristic growth on media such as Blood agar and MacConkey agar...
October 2018: Journal of Laboratory Physicians
Roseane Galdioli Nava, Mariana Oliveira-Silva, Rafael Nakamura-Silva, André Pitondo-Silva, Eliana Carolina Vespero
OBJECTIVES: The aim of this study was to investigate the blaNDM gene, the pathogenic potential and antimicrobial resistance in clinical isolates of carbapenem-resistant Klebsiella pneumoniae isolated from patients admitted to the University Hospital of Londrina between January 2014 and March 2017. METHODS: blaNDM-1 and virulence genes were investigated using conventional PCR methods Antimicrobial susceptibility tests were performed the disk diffusion method according to the standards described in CLSI 2017...
November 26, 2018: International Journal of Infectious Diseases: IJID
Yu Jin Park, Nguyen Le Phuong, Naina Adren Pinto, Mi Jeong Kwon, Roshan D'Souza, Jung-Hyun Byun, Heungsup Sung, Dongeun Yong
Background: Small colony and capnophilic variant cases have been separately reported, but there has been no reports of their simultaneous presence in one isolate. We report a case of Escherichia coli with coexpressed small colony and capnophilic phenotypes causing misidentification in automated biochemical kits and non-reactions in antimicrobial susceptibility test cards. Case presentation: An 86-year-old woman developed urinary tract infection from a strain of Escherichia coli with SCV and capnophilic phenotypes in co-existence...
2018: Antimicrobial Resistance and Infection Control
Simon Thompson, Douglas Chesher
Lot-to-lot variation affecting calibrators and reagents is a frequent challenge that limits the laboratory's ability to produce consistent results over time. This variation is not without clinical consequence and there are several well-documented examples of adverse clinical outcomes. It is important that laboratories have procedures in place for quantification of this inaccuracy, and for determining whether the amount of variation is acceptable for the release of patient results. Various approaches have been taken to the assessment of new lots, including the evaluation protocol published by the Clinical and Laboratory Standards Institute (CLSI)...
May 2018: Clinical Biochemist. Reviews
Raheela Bano, Adeel Arsalan
Antibiotics are not only used in morbidity but also help in prevention of infection. The irrational use of broad spectrum antibiotics is now increasing the resistance against pathogens. This present study has been carried out to evaluate the in-vitro antibacterial effect of levofloxacin against clinical isolates. According to Clinical and Laboratory Standards Institute (CLSI) guidelines, minimum inhibitory concentrations 90% (MIC90 ) of the levofloxacin tested were evaluated by an agar dilution method. Total 63 clinical isolates Staphylococcus aureus (n=34) and Escherichia coli (n=29) were collected from different hospitals at Karachi and were evaluated MIC90 of eleven different brands of levofloxacin tablet (250 mg)...
November 2018: Pakistan Journal of Pharmaceutical Sciences
Yuanqing Yang, Hongmin Jiang, Aiguo Tang, Zhongyuan Xiang
BACKGROUD: Reference intervals (RIs) of clinical laboratory indexes are important basis for interpretation of corresponding test results. While elevated homocysteine (HCY) level is a risk factor of some severe gestational diseases, HCY RIs for pregnant women have not been reported so far. The current use of HCY RIs established for general population in pregnant women may challenge clinicians' judgment. This study aims to investigate the changes of serum HCY levels during pregnancy and establish the RIs of serum HCY in healthy pregnant Chinese women to provide valuable data to clinicians and enable the provision of more appropriate therapy...
November 20, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
Asieh Taji, Hamid Heidari, Hadi Sedigh Ebrahim-Saraie, Jamal Sarvari, Mohammad Motamedifar
Multiple drug-resistant enterococci are major cause of healthcare-associated infections due to their antibiotic resistance traits. Among them, Enterococcus faecalis is an important opportunistic pathogen causing various hospital-acquired infections. A total of 53 E. faecalis isolates were obtained from various infections. They were identified by phenotypic and genotypic methods. Determination of antimicrobial resistance patterns was done according to CLSI guidelines. The isolates that were non-susceptible to at least one agent in ≥3 antimicrobial categories were defined as multidrug-resistant (MDR)...
November 22, 2018: Acta Microbiologica et Immunologica Hungarica
Bo-Young Hsieh, Wei-Hsun Chao, Yi-Jing Xue, Jyh-Mirn Lai
The aim of this study was to establish a ketoconazole susceptibility test for Malassezia pachydermatis using modified Leeming⁻Notman agar (mLNA). The susceptibilities of 33 M. pachydermatis isolates obtained by modified CLSI M27-A3 method were compared with the results by disk diffusion method, which used different concentrations of ketoconazole on 6 mm diameter paper disks. Results showed that 93.9% (31/33) of the minimum inhibitory concentration (MIC) values obtained from both methods were similar (consistent with two methods within 2 dilutions)...
November 16, 2018: Journal of Fungi (Basel, Switzerland)
Atossa Ghasemnejad, Monir Doudi, Nour Amirmozafari
Klebsiella pneumoniae carbapenemase (KPC) have become a major therapeutic challenge because of its increasingly fast dissemination throughout the world. Accurate detection of KPC is essential for optimal treatment. The Clinical and Laboratory Standards Institutes (CLSI) for fast detection of KPC producers currently recommend Modified Hodge Test (MHT) and Carba NP test. MHT can directly detect carbapenemase production in Enterobacteriaceae isolates. The current study was conducted to evaluate the capacity of MHT with two carbapenem disks for accurate detection of KPC...
2018: Polish Journal of Microbiology
Ana Valero, Arantxa Isla, Alicia Rodríguez-Gascón, Begoña Calvo, Andrés Canut, María Ángeles Solinís
INTRODUCTION: To evaluate the changes in the susceptibility of Pseudomonas aeruginosa over time (2000-2017) against antimicrobials used in an intensive care unit of a Spanish tertiary hospital, and to compare them with the antimicrobial activity considering theoretical pharmacokinetic/pharmacodynamic (PK/PD) criteria. The influence of the method for handling duplicate isolates to quantify susceptibility rates was also evaluated. METHODS: The susceptibility was studied considering the Clinical and Laboratory Standards Institute (CLSI) breakpoints...
November 15, 2018: Enfermedades Infecciosas y Microbiología Clínica
Michael J Dowzicky, Eva Chmelařová
OBJECTIVES: The Tigecycline Evaluation and Surveillance Trial (T.E.S.T.) is a global surveillance program that monitors the in vitro activity of a panel of antimicrobials against clinically important isolates. Data for Gram-positive and Gram-negative isolates collected in Eastern Europe between 2011 and 2016 are presented. METHODS: MICs were determined by broth microdilution using CLSI guidelines. Antimicrobial susceptibility was determined using European Committee on Antimicrobial Susceptibility Testing breakpoints...
November 13, 2018: Journal of Global Antimicrobial Resistance
Jung Hyun Byun, Myungsook Kim, Yangsoon Lee, Kyungwon Lee, Yunsop Chong
BACKGROUND: Anaerobic bacterial resistance trends may vary across regions or institutions. Regional susceptibility patterns are pivotal in the empirical treatment of anaerobic infections. We determined the antimicrobial resistance patterns of clinically important anaerobic bacteria, including recently named or renamed anaerobes. METHODS: A total of 521 non-duplicated clinical isolates of anaerobic bacteria were collected from a tertiary-care hospital in Korea between 2014 and 2016...
March 2019: Annals of Laboratory Medicine
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