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Sonal Sekhar M, Unnikrishnan M K, Gabriel Sunil Rodrigues, Navya Vyas, Chiranjay Mukhopadhyay
PURPOSE: Diabetic foot infections (DFIs) are major public health concerns. In the view of the ever increasing burden of multidrug-resistant (MDR) organisms, improving the use of antimicrobial agents (AMAs) is a national priority. Objective of the study was to determine antimicrobial susceptibility (AMS) pattern of aerobes in DFIs. METHODS: A cross-sectional study was conducted for a period of 3 years at Department of Surgery, Kasturba hospital, Manipal, India during which, 260 DFUs patients admitted in the general surgery wards were enrolled for the study...
July 11, 2018: Foot
J N Vieira, C L Gonçalves, J P V Villarreal, V M Gonçalves, R G Lund, R A Freitag, A F Silva, P S Nascente
The aims of this research were: evaluate the chemical composition and the cytotoxicity of the Cuminum cyminum (cumin), Anethum graveolens (dill), Pimpinella anisum (anise) and Foeniculum vulgare (fennel) essential oils, as well as their antifungal activity in vitro against ten Candida spp. isolates. The chemical composition of the oils was analyzed by means of gas chromatography coupled with mass spectrometry (GC/MS). The cytotoxicity assays were performed, using the cell proliferation reagent WST-1 in L929 mouse fibroblasts (20x103 well-1)...
October 11, 2018: Brazilian Journal of Biology, Revista Brasleira de Biologia
A Espinel-Ingroff, J Turnidge, A Alastruey-Izquierdo, F Botterel, E Canton, C Castro, Y-C Chen, Y Chen, E Chryssanthou, E Dannaoui, G Garcia-Effron, G M Gonzalez, N P Govender, J Guinea, S Kidd, M Lackner, C Lass-Flörl, M J Linares-Sicilia, L López-Soria, R Magobo, T Pelaez, G Quindós, M A Rodriguez-Iglesia, M A Ruiz, F Sánchez-Reus, M Sanguinetti, R Shields, P Szweda, A Tortorano, N L Wengenack, S Bramati, C Cavanna, C DeLuca, M Gelmi, A Grancini, G Lombardi, J Meletiadis, C E Negri, M Passera, J Peman, A Prigitano, E Sala, M Tejada
Although the Sensitrite Yeast-One (SYO) and Etest methods are widely utilized, interpretive criteria are not available for triazole susceptibility testing of Candida or Aspergillus species. We collected fluconazole, itraconazole, posaconazole and voriconazole SYO and Etest MICs from 39 laboratories representing all continents for (method-agent-dependent): 11,171 Candida albicans , 215 C. dubliniensis , 4,418 C. glabrata species complex (SC), 157 C. ( Meyerozyma ) guilliermondii , 676 C. krusei ( Pichia kudriavzevii ), 298 C ( Clavispora ) lusitaniae , 911 and 3,691 C...
October 15, 2018: Antimicrobial Agents and Chemotherapy
Helio S Sader, Robert K Flamm, Jennifer M Streit, Cecilia G Carvalhaes, Rodrigo E Mendes
OBJECTIVE: Evaluate the potency and spectrum of ceftaroline and comparator agents tested against contemporary bacteria isolated from patients with community-acquired bacterial pneumonia (CABP) in Europe (EUR), Asia-Pacific (APAC), and Latin America (LATAM). METHODS: A total of 4,321 bacterial isolates were consecutively collected by the SENTRY Antimicrobial Surveillance Program in 2015-2017 from 65 medical centres located in western EUR (W-EUR; 21 centres in 10 nations), eastern EUR and the Mediterranean region (E-EUR; 15 centres in 11 nations), APAC (18 centres in 9 nations), and LATAM (11 centres in 9 nations)...
October 10, 2018: International Journal of Infectious Diseases: IJID
Deepanshi Mishra, Gita Satpathy, Rohan Chawla, Pradeep Venkatesh, Nishat Hussain Ahmed, Subrat Kumar Panda
BACKGROUND: Endophthalmitis, a sight-threatening intraocular infection, can be of postsurgical, post-traumatic or endogenous origin. Laboratory diagnosis-based appropriate therapy can be vision-saving. Conventional culture-based laboratory diagnosis takes time and lacks sensitivity. In this study a broad-range PCR assay was assessed against conventional and automated culture methods in vitreous specimens for accurate microbiological diagnosis. AIMS: To use broad-range PCR assay targeting 16S ribosomal RNA (rRNA) region of bacteria and to assess its performance vis-à-vis conventional and automated culture methods in the laboratory diagnosis of endophthalmitis...
October 12, 2018: British Journal of Ophthalmology
David J Farrell, Rodrigo E Mendes, Mekki Bensaci
Staphylococcus lugdunensis and Staphylococcus haemolyticus are unique among CoNS in that the former often causes aggressive disease, while the latter consistently exhibits high rates of multidrug resistance. We evaluated the in vitro susceptibility of contemporary (2012-2013) isolates from both pathogens to tedizolid and comparators, using standard methodology. Results were interpreted using CLSI and EUCAST breakpoints. Overall, 106 S. lugdunensis and 103 S. haemolyticus isolates were collected from 51 medical centers in the United States and 30 centers in 18 European countries...
August 23, 2018: Diagnostic Microbiology and Infectious Disease
Maryam Raeispour, Reza Ranjbar
Background: The way of treating different types of infectious diseases is really important. Using genotyping method, we can determine the genetic relatedness between the organisms with different resistance profile from different sources. The aim of this study was to determine antibiotic resistance and genotyping of uropathogenic Escherichia coli (UPEC) strains using pulsed field gel electrophoresis (PFGE). Method: Escherichia coli ( E. coli ) strains were recovered from the patients with urinary tract infections (UTI) whom admitted in several major hospitals in Tehran...
2018: Antimicrobial Resistance and Infection Control
Gasal M Hashim, Saad B Almasaudi, Esam Azhar, Soad K Al Jaouni, Steve Harakeh
Introduction: A number of plant species, including Cymbopogon schoenanthus , are traditionally used for the treatment of various diseases. C. schoenanthus is currently, traded in the Saudi markets, and thought to have medicinal value. This study aimed at investigating the biological activities of C. schoenanthus against both Gram-positive and Gram-negative bacteria and to identify its chemical ingredients. Materials and methods: The inhibitory effects of water extracts of C...
November 2017: Saudi Journal of Biological Sciences
Jonathan Lellouche, David Schwartz, Noy Elmalech, Maayan Amar Ben Dalak, Elizabeth Temkin, Mical Paul, Yuval Geffen, Dafna Yahav, Noa Eliakim-Raz, Emanuele Durante-Mangoni, Domenico Iossa, Mariano Bernardo, George L Daikos, Anna Skiada, Angeliki Pantazatou, Anastasia Antoniadou, Johan W Mouton, Yehuda Carmeli
OBJECTIVES: The rise in carbapenem resistance among Gram-negative bacteria has renewed interest in colistin. Recently, the EUCAST-CLSI Polymyxin Breakpoints Working Group declared that broth microdilution (BMD) is the only valid method for colistin susceptibility testing. BMD is not easily incorporated into the routine work of clinical laboratories, and usually this test is incorporated serially, resulting in delayed susceptibility reporting. We tested a strategy of combining VITEK® 2 with a 2μg/ml colistin agar dilution (VITEK® 2/AD) screening plate to improve performance and time to reporting of colistin susceptibility...
October 3, 2018: Clinical Microbiology and Infection
Raül Rigo-Bonnin, Francesca Canalias
BACKGROUND: Due to the high inter-variability in immunosuppressants pharmacokinetics, therapeutic drug monitoring of these drugs is essential in order to minimize the risk of rejection after organ transplantation. Thus, results facilitated to clinicians by clinical laboratories for these drugs should be as reliable as possible. The knowledge of metrological traceability and performing compatibility studies can allow ensuring reliability of these results, mainly, when laboratories introduce substantial changes in their measurement procedures, e...
October 3, 2018: Clinical Biochemistry
Nesrin Ghanem-Zoubi, Danny Zorbavel, Johad Khoury, Yuval Geffen, Majd Qasum, Svetlana Predescu, Mical Paul
To evaluate the association between appropriate antifungal treatment and mortality among patients with candidemia using different breakpoint definitions. In a retrospective study, we included all adults with candidemia in a tertiary center between 2009 and 2015. We defined three versions of appropriate (covering) antifungal treatment, according to Clinical and Laboratory Standards Institute (CLSI) 2008, CLSI 2012, and European Committee on Antimicrobial Susceptibility Testing (EUCAST) (2017 update) breakpoints...
October 3, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Patricia J Simner, Yehudit Bergman, Marisol Trejo, Ava A Roberts, Remy Marayan, Tsigereda Tekle, Shelley Campeau, Abida Kazmi, Drew Bell, Shawna Lewis, Pranita D Tamma, Romney Humphries, Janet A Hindler
Background: Limited methods for colistin minimum inhibitory concentration (MIC) determination are available to clinical microbiology laboratories. The purpose of this study was to evaluate the accuracy of the colistin broth disk elution (CBDE) test as compared to broth microdilution (BMD) for identifying colistin MICs. Methods: CBDE was compared to colistin BMD using a collection of Gram-negative bacilli tested at two U.S. microbiology laboratories. Isolates tested included 121 retrospective clinical isolates, 45 prospective clinical isolates, and 6 mcr-1 positive E...
October 3, 2018: Journal of Clinical Microbiology
Santi M Mandal, Subhanil Chakraborty, Santanu Sahoo, Smritikona Pyne, Samaresh Ghosh, Ranadhir Chakraborty
BACKGROUND: The need for suitable antibacterial agents effective against Multi-drug resistant Gram-negative bacteria is acknowledged globally. The present study was designed to evaluate possible antibacterial potential of an extracted compound from edible flowers of Moringa oleifera. METHODS: Five different solvents were used for preparing dried flower extracts. The most effective extract was subjected to fractionation and further isolation of the active compound with highest antibacterial effect was obtained using TLC, Column Chromatography and reverse phase- HPLC...
October 1, 2018: Infectious Disorders Drug Targets
Ananta Khurana, Aradhana Masih, Anuradha Chowdhary, Kabir Sardana, Sagar Borker, Aastha Gupta, R K Gautam, P K Sharma, Dhruv Jain
Recalcitrant dermatophytoses are on the rise in India. High MICs of terbinafine (TRB) and squalene epoxidase ( SQLE ) mutations conferring resistance in Trichophyton spp have been recently documented. However, studies correlating laboratory data with clinical response to TRB in tinea corporis/cruris are lacking. This study investigated the clinico-mycological profile of 85 tinea corporis/cruris patients, and performed antifungal susceptibility testing by CLSI micro-broth dilution and SQLE mutation analysis of isolates obtained and correlated these with the response to TRB...
October 1, 2018: Antimicrobial Agents and Chemotherapy
Dongmei Gu, Yang Wang, Bingcheng Ren, Le Wang, Ke Zhang, Yuhua Yuan
BACKGROUND: The reliability of bilirubin measurements is not satisfactory due to the marked variability of methodologies since discovery. METHODS: Imprecision of the three analytical systems (diazo method, vanadate oxidase method, and dry chemistry method) were calculated in accordance with CLSI guidelines EP15-A2. According to Clinical and Laboratory Standards Institute (CLSI) guideline (EP9-A2), 40 analytes were detected to evaluate method performance and comparability among three routine measurements for serum bilirubin in a China laboratory...
September 1, 2018: Clinical Laboratory
Hui Zhang, Haishen Kong, Yunsong Yu, Anhua Wu, Qiong Duan, Xiaofeng Jiang, Shufang Zhang, Ziyong Sun, Yuxing Ni, Weiping Wang, Yong Wang, Kang Liao, Huayin Li, Chunxia Yang, Wenxiang Huang, Bingdong Gui, Bin Shan, Robert Badal, Qiwen Yang, Yingchun Xu
BACKGROUND: To evaluate the susceptibility rates of aerobic and facultative Gram-negative bacterial isolates from Chinese intra-abdominal infections (IAI) and urinary tract infections (UTI) focusing on carbapenems and comparing their effectiveness between 2014 and 2015. METHODS: A total of 2318 strains in 2015 (1483 from IAI and 835 from UTI) and 2374 strains in 2014 (1438 from IAI and 936 from UTI) were included in the analysis. Antimicrobial susceptibilities were determined at a central laboratory using CLSI broth microdilution and interpretive standards...
September 29, 2018: BMC Infectious Diseases
Andrew Mead, Peter Lees, John Mitchell, Andrew Rycroft, Joseph F Standing, Pierre-Louis Toutain, Ludovic Pelligand
For clinical isolates of bovine Mannheimia haemolytica and Pasteurella multocida, this study reports minimum inhibitory concentration (MIC) differences for tetracycline, oxytetracycline and doxycycline between cation-adjusted Mueller-Hinton broth (CAMHB), foetal bovine serum (FBS) and Roswell Park Memorial Institute (RPMI) medium. MICs were determined according to CLSI standards and additionally using five overlapping sets of twofold dilutions. Matrix effect: (a) free drug MICs and minimum bactericidal concentrations (MBC) for all drugs were significantly higher in FBS than in CAMHB for both pathogens (p < 0...
September 28, 2018: Journal of Veterinary Pharmacology and Therapeutics
David B Huang, Sophie Magnet, Stefania De Angelis, Thomas L Holland, Thomas M File, Matthew Dryden, G Ralph Corey, Antoni Torres, Mark H Wilcox
Iclaprim is a diaminopyrimidine, which inhibits bacterial dihydrofolate reductase, and surveillance data prior to 2006 suggested that iclaprim was active against Gram-positive pathogens including emerging drug-resistant pathogens. In an era of increasing antimicrobial resistance, we undertook testing iclaprim and comparators against 931 Gram-positive clinical isolates from the United States and Europe collected between 2015 and 2016. Susceptibility testing was performed according to the Clinical and Laboratory Standards Institute (CLSI) guidelines...
September 10, 2018: Diagnostic Microbiology and Infectious Disease
Eun Hye Cho, Rihwa Choi, Eun-Suk Kang, Hyung-Doo Park
IgG consists of four subclasses: IgG1, IgG2, IgG3, and IgG4. Changes in the serum concentration of each subclass reflect different clinical situations, and quantification of each subclass is important to assess patients' clinical states. Herein, we evaluated the analytical performance of the SPAPLUS turbidimetric analyzer (The Binding Site, Birmingham, UK) for IgG subclass. Precision, linearity, comparison with the BNII system (Siemens Healthineers, Erlangen, Germany), and reference interval were assessed according to the Clinical and Laboratory Standards Institute (CLSI) guidelines...
September 27, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Helio S Sader, Paul R Rhomberg, Timothy B Doyle, Robert K Flamm, Rodrigo E Mendes
We assessed ceftaroline disk diffusion breakpoints for Staphylococcus aureus when applying revised Clinical and Laboratory Standards Institute (CLSI) ceftaroline MIC breakpoints. Disk-MIC correlation was evaluated by testing a challenge collection ( n = 158) of methicillin-resistant S. aureus (MRSA) composed of 106 randomly selected isolates plus 52 isolates with decreased susceptibility to ceftaroline (MIC, 1-16 μg/mL). Disk diffusion was performed with 30-μg disks and Mueller-Hinton agar from 2 manufacturers each...
September 26, 2018: Journal of Clinical Microbiology
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