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Sophie Chauvet, Lubka T Roumenina, Pierre Aucouturier, Maria-Chiara Marinozzi, Marie-Agnès Dragon-Durey, Alexandre Karras, Yahsou Delmas, Moglie Le Quintrec, Dominique Guerrot, Noémie Jourde-Chiche, David Ribes, Pierre Ronco, Frank Bridoux, Véronique Fremeaux-Bacchi
C3 glomerulopathy (C3G) results from acquired or genetic abnormalities in the complement alternative pathway (AP). C3G with monoclonal immunoglobulin (MIg-C3G) was recently included in the spectrum of "monoclonal gammopathy of renal significance." However, mechanisms of complement dysregulation in MIg-C3G are not described and the pathogenic effect of the monoclonal immunoglobulin is not understood. The purpose of this study was to investigate the mechanisms of complement dysregulation in a cohort of 41 patients with MIg-C3G...
2018: Frontiers in Immunology
Alexander Dimitri Yermanos, Andreas Kevin Dounas, Tanja Stadler, Annette Oxenius, Sai T Reddy
Antibody evolution studies have been traditionally limited to either tracing a single clonal lineage (B cells derived from a single V-(D)-J recombination) over time or examining bulk functionality changes (e.g., tracing serum polyclonal antibody proteins). Studying a single B cell disregards the majority of the humoral immune response, whereas bulk functional studies lack the necessary resolution to analyze the co-existing clonal diversity. Recent advances in high-throughput sequencing (HTS) technologies and bioinformatics have made it possible to examine multiple co-evolving antibody monoclonal lineages within the context of a single repertoire...
2018: Frontiers in Immunology
Bhawna Poonia, Natarajan Ayithan, Madhuparna Nandi, Henry Masur, Shyam Kottilil
Spontaneous or treatment induced seroconversion in chronic HBV infection is rare and generation of anti-HBs antibodies is the current goal of HBV therapeutics. Here we investigated B and follicular T helper (Tfh) cell defects that persist in HBV infection despite long-term nucleos(t)ide analog (NUC) treatment and possible mechanisms behind them. RNA sequencing revealed that patient B cells have upregulated expression of multiple inhibitory receptors including members of FcRL family and downregulation of genes involved in antigen presentation...
October 17, 2018: Scientific Reports
Mohammed Fawzy El-Kamel, Mohamed Khaled Selim, Mamdouh Morsy Abdel Gawad
Rosai-Dorfman disease or sinus histiocytosis with massive lymphadenopathy is a benign lympho-histiocytic proliferative disorder initially described with bilateral painless lymphadenopathy (90 %), fever, leukocytosis, elevated ESR, anemia, and polyclonal hypergammaglobulinemia (90 %). Extranodal forms occur in 43% of cases, the skin being the most common site. Around 10% of patients have skin lesions and in 3%, the disease is limited exclusively to the skin. Here, we report a male patient who presented with pure cutaneous lesions which mimic eruptive xanthoma clinically...
October 17, 2018: Indian Journal of Dermatology, Venereology and Leprology
Jaroslava Dekkerová, Jose L Lopez-Ribot, Helena Bujdáková
Fungal biofilm has remained a serious medical problem that complicates treatment of mycoses. In particular, once biofilms are formed, they display high levels of resistance against most common antifungals. Candida auris is currently considered as a serious emerging fungal pathogen frequently exhibiting high levels of resistance to antifungals. Recent studies have confirmed that C. auris shares similarity with Candida albicans in regards to virulence-associated proteins involved in adherence and biofilm development...
October 16, 2018: European Journal of Clinical Microbiology & Infectious Diseases
Mepur H Ravindranath, Edward J Filippone, Grace Mahowald, Carly Callender, Adarsh Babu, Susan Saidman, Soldano Ferrone
The accurate measurement of anti-HLA alloantibodies in transplant candidates is required for determining the degree of sensitization and for the listing of unacceptable antigens for organ allocation. Both the configuration of the HLA molecules coated on the beads and the nature of detection antibodies may impede assessment of the presence and strength of anti-HLA IgG- with the Luminex single-antigen-bead assay. Sera antibodies of the end-stage renal disease patients were compared using LIFECODES (LC) and LABScreen (LS) beadsets monitored with polyclonal-Fab (IgHPolyFab) and monoclonal-IgG (FcMonoIgG) second antibodies...
October 16, 2018: Immunologic Research
Sakshi Gera, Rajan R Dighe
The transduction of signal by the Notch receptors to the intracellular domain is highly regulated and relies on binding of the ligands to the Epidermal growth factor like repeats (ELRs) of the receptor's extracellular domain. Both canonical and non-canonical ligands are known to interact with different ELRs and activate Notch receptors. The aim of this study was to investigate the interaction of a soluble non-canonical ligand, Y box-1 (Yb-1) with Notch3 receptor ELRs. Polyclonal antibodies were employed as the novel tools to identify the binding site of this ligand...
October 12, 2018: Archives of Biochemistry and Biophysics
Maria Sánchez-Purrà, Biel Roig-Solvas, Cristina Rodriguez-Quijada, Brianna M Leonardo, Kimberly Hamad-Schifferli
We report a quantitative evaluation of the choice of reporters for multiplexed surface-enhanced Raman spectroscopy (SERS). An initial library consisted of 15 reporter molecules that included commonly used Raman dyes, thiolated reporters, and other small molecules. We used a correlation matrix to downselect Raman reporters from the library to choose five candidates: 1,2-bis(4-pyridyl)ethylene, 4-mercaptobenzoic acid, 3,5-dichlorobenzenthiol, pentachlorothiophenol, and 5,5'-dithiobis(2-nitrobenzoic acid). We evaluated the ability to distinguish the five SERS reporters in a dipstick immunoassay for the biomarker human IgG...
September 30, 2018: ACS Omega
Miriam Salvador-Bescós, Yolanda Gil-Ramírez, Amaia Zúñiga-Ripa, Estrella Martínez-Gómez, María J de Miguel, Pilar M Muñoz, Axel Cloeckaert, Michel S Zygmunt, Ignacio Moriyón, Maite Iriarte, Raquel Conde-Álvarez
Brucellosis, an infectious disease caused by Brucella , is one of the most extended bacterial zoonosis in the world and an important cause of economic losses and human suffering. The lipopolysaccharide (LPS) of Brucella plays a major role in virulence as it impairs normal recognition by the innate immune system and delays the immune response. The LPS core is a branched structure involved in resistance to complement and polycationic peptides, and mutants in glycosyltransferases required for the synthesis of the lateral branch not linked to the O -polysaccharide (O-PS) are attenuated and have been proposed as vaccine candidates...
2018: Frontiers in Microbiology
S W G J W Chinthaka, R L Satarasinghe, S Senanayake, W A P S R Weerarathne, A A M Anfaz, M P Deraniyagala
BACKGROUND: Lymphadenopathy is not an uncommon presentation of adult onset Still's disease: it is present in up to two thirds of patients with adult onset Still's disease. The characteristic appearance of lymphadenopathy is described as intense, paracortical immunoblastic hyperplasia. Changes in light microscopy may resemble lymphoma, but immunohistochemistry reveals a benign polyclonal B cell hyperplasia. CASE PRESENTATION: We describe a 67-year-old Sri Lankan woman who manifested relapsing prolonged fever, raised inflammatory markers, arthralgia, myalgia, transient skin rash, and cervical lymphadenopathy histologically characterized by noncaseating granulomatous adenitis with central suppuration...
October 15, 2018: Journal of Medical Case Reports
Xiukun Cui, Jiuli Han, Jing Li, Wen-Wen Cui, Dan-Dan Wu, Shiyuan Liu, Wenxian Xue, Xiang-Xiang Wang, Yuanfang Ma, Jing Zhang, Jun Zhang, Hongmei Mu, Fengyan Zhang, Yanzhong Hu
Dysfunction of HSF4 is associated with congenital cataracts. HSF4 transcription activity is turned on and regulated by phosphorylation during early postnatal lens development. Our previous data suggested that mutation HSF4b/S299 A can upregulate HSF4 transcription activity in vitro, but the biological significance of posttranslational modification on HSF4/S299 during lens development remains unclear. Here, we found that the mutation HSF4/S299 A can upregulate the expression of HSP25 and alpha B-crystallin at both protein and mRNA levels in mouse the lens epithelial cell line, but HSF4/S299D does not...
October 11, 2018: International Journal of Biochemistry & Cell Biology
Sarah H M Hedberg, Jonathan Rapley, Jonathan M Haigh, Daryl R Williams
Protein aggregation can be a major problem in the manufacturing of new biopharmaceuticals and there is a desirability for development of techniques that can predict the behaviour of new biopharmaceuticals early on in the development process. A technique that can be used to predict aggregation is self-interaction chromatography that is used to determine the second virial coefficient, B22 , but one of the limitations includes the need to immobilise every protein of interest. In this study a related technique, cross interaction chromatography (CIC), is evaluated which overcomes this limitation...
October 11, 2018: European Journal of Pharmaceutics and Biopharmaceutics
Alex Jesús Hernández-Granados, Moisés Armides Franco-Molina, Erika Evangelina Coronado-Cerda, Pablo Zapata-Benavides, Edgar Mendoza Gamboa, Yareellys Ramos-Zayas, Silvia Elena Santana-Krímskaya, Cristina Rodríguez-Padilla
Whole tumor cell lysates consist of a mixture of tumor antigens and danger associated molecular patterns (DAMPs) that can be used for dendritic cell maturation and consequently for the activation of a polyclonal T cell-specific tumor response. We evaluated the in vitro efficacy of three different preparations of canine transmissible venereal tumor (TVT) cell lysates: hypochlorous acid-whole tumor cell lysates (HOCl-L), heat shock-whole tumor cell lysates (HS-L), and freeze-thaw cycles-whole tumor cell lysates (FT-L) for the maturation of canine-derived dendritic cells...
October 5, 2018: Research in Veterinary Science
S Emtenani, H Yuan, C Lin, M Pan, J E Hundt, E Schmidt, L Komorowski, J R Stanley, C M Hammers
BACKGROUND: Bullous pemphigoid (BP) is the most common autoimmune subepidermal blistering skin disease. Two antigens were identified as targets of circulating autoantibodies (abs), BP180 and BP230, with BP180 being a critical transmembrane adhesion protein of basal keratinocytes of the epidermis. The non-collagenous domain 16A (NC16A) of BP180 is the immunodominant epitope in BP patients, and anti-BP180-NC16A immunoglobulin G (IgG) abs correlate to disease activity. Routine serologic testing and follow-up of BP relies on indirect immunofluorescence (IIF) of serum abs, commonly performed on monkey esophagus (ME), and/or ELISA testing on recombinantly produced fragments of BP180 and BP230 (BP180-NC16A, BP230-C/N)...
October 13, 2018: British Journal of Dermatology
Dandan Liang, Shaoshan Liang, Feng Xu, Mingchao Zhang, Xiaomei Li, Yiyao Tu, Zhihong Liu, Caihong Zeng
AIM: To explore the clinicopathological characteristics of patients with anti-GBM antibody-negative anti-GBM disease. METHODS: The clinical and renal pathological findings were retrospectively studied in 19 patients. All patients met the following inclusion criteria: linear GBM IgG deposition on immunofluorescence(IF); and lack of serum anti-GBM antibodies by ELISA and indirect immunofluorescence assay. RESULTS: There were 17 male and two female patients, with a median age of 36 years (range 15-61 years)...
October 12, 2018: Journal of Clinical Pathology
Michela Consonni, Paolo Dellabona, Giulia Casorati
Adoptive cell therapy (ACT) using tumor-specific "conventional" MHC-restricted T cells obtained from tumor-infiltrating lymphocytes, or derived ex vivo by either antigen-specific expansion or genetic engineering of polyclonal T cell populations, shows great promise for cancer treatment. However, the wide applicability of this therapy finds limits in the high polymorphism of MHC molecules that restricts the use in the autologous context. CD1 antigen presenting molecules are nonpolymorphic and specialized for lipid antigen presentation to T cells...
October 8, 2018: Molecular Immunology
Noam Levin, Hadas Weinstein-Marom, Aviad Pato, Orit Itzhaki, Michal J Besser, Galit Eisenberg, Tamar Peretz, Michal Lotem, Gideon Gross
New strategies for augmenting the actual performance of therapeutic T cells in vivo are needed for improving clinical outcome of adoptive cell therapy. Cumulative findings suggest that CD40 plays an intrinsic role in T cell costimulation. Recently, we demonstrated the ability of truncated, auto-oligomerizing CD40 derivatives to induce strong activation of APCs in a ligand-independent manner. We reasoned that constitutively active CD40 (caCD40) can similarly exert enhancing effects on human antitumor T cells...
October 10, 2018: Journal of Immunology: Official Journal of the American Association of Immunologists
Surender Khurana, Elizabeth M Coyle, Jody Manischewitz, Lisa R King, Jin Gao, Ronald N Germain, Pamela L Schwartzberg, John S Tsang, Hana Golding
Immune responses to inactivated vaccines against avian influenza are poor due in part to lack of immune memory. Adjuvants significantly increased virus neutralizing titers. We performed comprehensive analyses of polyclonal antibody responses following FDA-approved adjuvanted H5N1-A/Indonesia vaccine, administered in presence or absence of AS03. Using Whole Genome Fragment Phage Display Libraries, we observed that AS03 induced antibody epitope diversity to viral hemagglutinin (HA) and neuraminidase compared with unadjuvanted vaccine...
2018: NPJ Vaccines
John H Beigel
PURPOSE OF REVIEW: This review summarizes past and ongoing efforts for using polyclonal and monoclonal antibodies for the treatment of influenza, and is focused on products that have entered clinical trials. RECENT FINDINGS: At least 3 polyclonal and 8 monoclonal antibody products have been tested in clinical trials for the treatment of influenza. Considered across the two classes of therapeutics, these products appear to be safe and well tolerated. However, the efficacy results have been mixed and inconclusive...
October 5, 2018: Current Opinion in Infectious Diseases
Boryana Borisova, Alfredo Sánchez, Paul E D Soto-Rodríguez, Abderrahmane Boujakhrout, María Arévalo-Villena, José M Pingarrón, Ana Briones-Pérez, Concepción Parrado, Reynaldo Villalonga
A sensitive and disposable amperometric immunosensor for Saccharomyces cerevisiae was constructed by using carbon screen-printed electrodes modified with propionic acid-functionalized graphene oxide as transduction element. The affinity-based biosensing interface was assembled by covalent immobilization of a specific polyclonal antibody on the carboxylate-enriched electrode surface via a water-soluble carbodiimide/N-hydroxysuccinimide coupling approach. A concanavalin A-peroxidase conjugate was further used as signaling element...
October 8, 2018: Analytical and Bioanalytical Chemistry
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