keyword
https://read.qxmd.com/read/38436764/dynamic-yap-expression-in-the-non-parenchymal-liver-cell-compartment-controls-heterologous-cell-communication
#21
JOURNAL ARTICLE
Kaijing Liu, Lilija Wehling, Shan Wan, Sofia M E Weiler, Marcell Tóth, David Ibberson, Silke Marhenke, Adnan Ali, Macrina Lam, Te Guo, Federico Pinna, Fabiola Pedrini, Amruta Damle-Vartak, Anne Dropmann, Fabian Rose, Silvia Colucci, Wenxiang Cheng, Michaela Bissinger, Jennifer Schmitt, Patrizia Birner, Tanja Poth, Peter Angel, Steven Dooley, Martina U Muckenthaler, Thomas Longerich, Arndt Vogel, Mathias Heikenwälder, Peter Schirmacher, Kai Breuhahn
INTRODUCTION: The Hippo pathway and its transcriptional effectors yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) are targets for cancer therapy. It is important to determine if the activation of one factor compensates for the inhibition of the other. Moreover, it is unknown if YAP/TAZ-directed perturbation affects cell-cell communication of non-malignant liver cells. MATERIALS AND METHODS: To investigate liver-specific phenotypes caused by YAP and TAZ inactivation, we generated mice with hepatocyte (HC) and biliary epithelial cell (BEC)-specific deletions for both factors (YAPKO, TAZKO and double knock-out (DKO))...
March 4, 2024: Cellular and Molecular Life Sciences: CMLS
https://read.qxmd.com/read/38435816/fibroblasts-and-endothelial-cells-interplay-drives-hypertrophic-scar-formation-insights-from-in-vitro-and-in-vivo-models
#22
JOURNAL ARTICLE
Yaxin Tan, Mengde Zhang, Yi Kong, Fanliang Zhang, Yuzhen Wang, Yuyan Huang, Wei Song, Zhao Li, Linhao Hou, Liting Liang, Xu Guo, Qinghua Liu, Yu Feng, Chao Zhang, Xiaobing Fu, Sha Huang
Hypertrophic scar formation is influenced by the intricate interplay between fibroblasts and endothelial cells. In this study, we investigated this relationship using in vitro and in vivo models. Clinical observations revealed distinct morphological changes and increased vascularity at pathological scar sites. Further analysis using OCTA, immunohistochemistry, and immunofluorescence confirmed the involvement of angiogenesis in scar formation. Our indirect co-culture systems demonstrated that endothelial cells enhance the proliferation and migration of fibroblasts through the secretion of cytokines including VEGF, PDGF, bFGF, and TGF-β...
March 2024: Bioengineering & Translational Medicine
https://read.qxmd.com/read/38434981/comprehensive-analysis-of-prognostic-value-and-immune-infiltration-of-regulator-of-chromosome-condensation-2-in-lung-adenocarcinoma
#23
JOURNAL ARTICLE
Hai Lin, Guofu Lin, Lanlan Lin, Jiansheng Yang, Dongyong Yang, Qinhui Lin, Yuan Xu, Yiming Zeng
Background: Lung adenocarcinoma (LUAD) incidence and mortality take the leading place of most malignancies. Previous studies have revealed the regulator of chromosome condensation 1 (RCC1) family members played an essential role during tumorigenesis. However, its biological functions in LUAD still need further investigation. Methods: Several databases were applied to explore potential effects of RCC1 family members on LUAD, such as Oncomine, GEPIA, and cBioPortal. Real-time PCR and immunohistochemistry were used to verify the expression of RCC2 in stage I LUAD...
2024: Journal of Cancer
https://read.qxmd.com/read/38434762/h-2-s-donor-s-propargyl-cysteine-for-skin-wound-healing-improvement-via-smart-transdermal-delivery
#24
JOURNAL ARTICLE
Xiaoqing Zhao, Yao Chen, Zhongxiao Lin, Xinyang Jin, Bolun Su, Xiaotong Liu, Mao Yang, Keyuan Chen, Menglin Zhu, Lei Wang, Yi Zhun Zhu
Hydrogen sulfide for wound healing has drawn a lot of attention recently. In this research, the S-propargyl-cysteine (SPRC), an endogenous H2 S donor, was loaded on carbomer hydrogel, and a copper sheet rat burn model was developed. Pathological changes in rat skin tissue were examined using hematoxylin-eosin (HE) and Masson staining. The immunohistochemistry (IHC) staining was performed to detect the expression of Collagen I (Col I) and Collagen III (Col III). The mRNA levels of interleukin (IL)-6, Col Iα2, Col IIIα1, tissue inhibitors of metalloproteinase (TIMP)-1, matrix metalloproteinase (MMP)-9, vascular endothelial growth factor (VEGF), and transforming growth factor (TGF)-β1 were examined by quantitative real-time chain polymerase reaction...
March 2024: MedComm
https://read.qxmd.com/read/38434376/plasma-derived-exosomes-contributes-to-endothelial-to-mesenchymal-transition-in-moyamoya-disease
#25
JOURNAL ARTICLE
Jilan Liu, Chao Chen, Xianyun Qin, Jingtong Wang, Bin Zhang, Feng Jin
BACKGROUND: Moyamoya disease (MMD) is a cerebrovascular disease with a high disability rate; however, its pathogenesis remains unknown. Endothelial-mesenchymal transition (EndMT) is the pathological basis of many vascular diseases; however, the key role of EndMT in MMD has not yet been reported. METHOD: We collected vascular tissues from three control samples and six patients with MMD to detect the expression of EndMT-related genes. To elucidate the mechanism of EndMT in MMD, we performed in vitro cell experiments...
March 15, 2024: Heliyon
https://read.qxmd.com/read/38433789/cyclic-high-negative-pressure-external-volume-expansion-reduces-daily-device-application-time-with-similar-effects-on-recipient-site-preparation-in-a-murine-model
#26
JOURNAL ARTICLE
Jinwook Jeong, Joseph Kyu-Hyung Park, Young Bin Choy, Jung Hee Shim, So Min Kang, Sun-Young Nam, Bakhtiyor Najmiddinov, Chan Yeong Heo
Introduction: Recipient site preparation using external volume expansion (EVE) increases graft survival in large-volume fat grafting. To improve patient compliance with using the device, we tested a new cyclic high negative-pressure (CHNP) mode that involves 1 h/day at -55 mm Hg, cycled between 1-second negative-pressure activation, followed by a 2-second deactivation period in an animal model. Material and Method: A miniaturized EVE device was applied to 30 8-week-old male Sprague-Dawley rats. The rats were assigned to 3 groups (no pressure for the control group, conventional -25 mm Hg for 8 h/day for conventional EVE, and CHNP mode for the CHNP group)...
February 2024: Plastic Surgery
https://read.qxmd.com/read/38430369/lipolysis-inhibition-improves-the-survival-of-fat-grafts-through-ameliorating-lipotoxicity-and-inflammation
#27
JOURNAL ARTICLE
Xingtang Niu, Yuchen Zhang, Zhuhao Lai, Xiaoqi Huang, Lingling Guo, Feng Lu, Yi Yuan, Jianhua Gao, Qiang Chang
Fat grafting is a promising technique for correcting soft tissue abnormalities, but oil cyst formation and graft fibrosis frequently impede the therapeutic benefit of fat grafting. The lipolysis of released oil droplets after grafting may make the inflammation and fibrosis in the grafts worse; therefore, by regulating adipose triglyceride lipase (ATGL) via Atglistatin (ATG) and Forskolin (FSK), we investigated the impact of lipolysis on fat grafts in this study. After being removed from the mice and chopped into small pieces, the subcutaneous fat from wild-type C57BL/6J mice was placed in three different solutions for two hours: serum-free cell culture medium, culture medium+FSK (50 μM), and culture medium+ATG (100 μM)...
March 15, 2024: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://read.qxmd.com/read/38429756/phf5a-promotes-esophageal-squamous-cell-carcinoma-progression-via-stabilizing-vegfa
#28
JOURNAL ARTICLE
Zhiwei Chang, Yongxu Jia, Ming Gao, Lijie Song, Weijie Zhang, Ruihua Zhao, Dandan Yu, Xiaolei Liu, Jing Li, Yanru Qin
BACKGROUND: Esophageal squamous cell carcinoma (ESCC) is the main subtype of esophageal cancer. Current therapeutic effect is far from satisfaction. Hence, identifying susceptible genes and potential targets is necessary for therapy of ESCC patients. METHODS: Plant homeodomain (PHD)-finger domain protein 5 A (PHF5A) expression in ESCC tissues was examined by immunohistochemistry. RNA interference was used for in vitro loss-of-function experiments. In vivo assay was performed using xenograft mice model by subcutaneous injection...
March 1, 2024: Biology Direct
https://read.qxmd.com/read/38423447/placental-differences-between-severe-fetal-growth-restriction-and-hypertensive-disorders-of-pregnancy-requiring-early-preterm-delivery-morphometric-analysis-of-the-villous-tree-supported-by-artificial-intelligence
#29
JOURNAL ARTICLE
Anna Jacobs, Saif I Al-Juboori, Evgenia Dobrinskikh, Matthew A Bolt, Mary D Sammel, Virginia Lijewski, Miriam D Post, James M Small, Emily J Su
BACKGROUND: The "great obstetrical syndromes" of fetal growth restriction and hypertensive disorders of pregnancy can occur individually or be interrelated. Placental pathologic findings often overlap between these conditions, regardless of whether one or both diagnoses are present. Quantification of placental villous structures in each of these settings may identify distinct differences in developmental pathways. OBJECTIVE: To determine how quantity and surface area of placental villi and vessels differ between severe, early-onset fetal growth restriction with absent/reversed umbilical artery Doppler indices, hypertensive disorders of pregnancy, and the two conditions combined among subjects with disease severity warranting early preterm delivery...
February 27, 2024: American Journal of Obstetrics and Gynecology
https://read.qxmd.com/read/38422899/hif1a-transcriptional-regulation-of-cox4i2-impacts-angiogenesis-in-pheochromocytoma
#30
JOURNAL ARTICLE
Xiuci Li, Ran Zhuo, Yongxin Mao, Chen Fang, Jun Dai, Lei Ye, Chenghe Wang, Fukang Sun
BACKGROUND: Pheochromocytoma (PCC) is a rare neuroendocrine tumor. Angiogenesis is primary contributing factor for tumorigenesis. Cytochrome c oxidase 4I2 (COX4I2) has been confirmed to take part in the progression of cancer. Hypoxia-inducible factor 1A (HIF1A) is the main regulatory factor for the steady-state response of hypoxia, involved in metabolism and angiogenesis. In this study, we intended to explore the functions of COX4I2 in PCC and the effect mechanism between HIF1A and COX4I2...
February 6, 2024: Biochemical and Biophysical Research Communications
https://read.qxmd.com/read/38418738/enhancing-vasculogenesis-in-dental-pulp-development-dpscs-ecs-communication-via-fn1-itga5-signaling
#31
JOURNAL ARTICLE
Tiankai Di, Chao Feng, Lulu Wang, Jinlong Xu, Yang Du, Baixiang Cheng, Yujiang Chen, Lian Wu
BACKGROUND: Dental pulp regeneration therapy is a challenge to achieve early vascularization during treatment. Studying the regulatory mechanisms of vascular formation during human dental pulp development may provide insights for related therapies. In this study, we utilized single-cell sequencing analysis to compare the gene expression of dental pulp stem cells (DPSCs) and vascular endothelial cells (ECs) from developing and mature dental pulps. METHOD: Immunohistochemistry, Western blot, and real-time polymerase chain reaction (RT-PCR) were used to detect fibronectin 1 (FN1) expression and molecules, such as PI3K/AKT...
February 28, 2024: Stem cell reviews and reports
https://read.qxmd.com/read/38417279/uterine-wound-healing-after-caesarean-section-a-systematic-review
#32
REVIEW
E Debras, P Capmas, C Maudot, P Chavatte-Palmer
The rate of caesarean section (CS) is increasing worldwide. Defects in uterine healing have a major gynaecological and obstetric impact (uterine rupture, caesarean scar defect, caesarean scar pregnancy, placenta accreta spectrum). The complex process of cellular uterine healing after surgery, and specifically after CS, remains poorly understood in contrast to skin wound healing. This literature review on uterine wound healing was mainly based on histological observations, particularly after CS. The primary objective of the review was to examine the effects of CS on uterine tissue at the cellular level, based on histological observations...
February 27, 2024: European Journal of Obstetrics, Gynecology, and Reproductive Biology
https://read.qxmd.com/read/38415447/quercetin-prevents-hspertension-in-dahl-salt-sensitive-rats-fed-a-high-salt-diet-through-balancing-endothelial-nitric-oxide-synthase-and-sirtuin-1
#33
JOURNAL ARTICLE
Guanji Wu, Fuqiang Liu, Qing Cui, Tao Zhang, Junjun Hao, Jianjun Bao
BACKGROUND: A high-salt diet is a leading dietary risk factor for elevated blood pressure and cardiovascular disease. Quercetin reportedly exhibits cardioprotective and antihypertensive therapeutic effects. OBJECTIVES: The objective of this study is to examine the effect of quercetin on high-salt dietinduced elevated blood pressure in Dahl salt-sensitive (SS) rats and determine the underlying molecular mechanism. MATERIALS AND METHODS: Rats of the Dahl SS and control SS-13 BN strains were separated into five groups, SS-13 BN rats fed a low-salt diet (BL group), SS-13 BN rats fed a high-salt diet (BH group), Dahl SS rats fed a low-salt diet (SL group), Dahl SS rats fed a high-salt diet (SH group), and SH rats treated with quercetin (SHQ group)...
February 19, 2024: Combinatorial Chemistry & High Throughput Screening
https://read.qxmd.com/read/38415159/multi-parametric-investigations-on-the-effects-of-vascular-disrupting-agents-based-on-a-platform-of-chorioallantoic-membrane-of-chick-embryos
#34
JOURNAL ARTICLE
Lei Chen, Mingpei Wang, Yuanbo Feng, Lingjie Gao, Jie Yu, Lei Geng, Yiyang Xie, Walter Coudyzer, Yue Li, Yicheng Ni
BACKGROUND: Vascular disrupting agents (VDAs) are known to specifically target preexisting tumoural vasculature. However, systemic side effects as safety or toxicity issues have been reported from clinical trials, which call for further preclinical investigations. The purpose is to gain insights into their non-specific off-targeting effects on normal vasculature and provide clues for exploring underlying molecular mechanisms. METHODS: Based on a recently introduced platform consisting laser speckle contrast imaging (LSCI), chick embryo chorioallantoic membrane (CAM), and assisted deep learning techniques, for evaluation of vasoactive medicines, hemodynamics on embryonic day 12 under constant intravascular infusion of two VDAs were qualitatively observed and quantitatively measured in real time for 30 min...
February 1, 2024: Quantitative Imaging in Medicine and Surgery
https://read.qxmd.com/read/38408759/phosphatidylserine-accelerates-wound-healing-and-reduces-necrosis-in-the-rats-growth-factor-activation
#35
JOURNAL ARTICLE
Partow Mirzaee Saffari, Pooria Asili, Sadaf Eshraghi, Ahad Muhammadnejad, Ahmad Reza Dehpour, Ramin Goudarzi, Alireza Partoazar
To examine the effect of topical phosphatidylserine (PS) on wound healing factors and tissue necrosis in in vivo models. Topical PS was applied to evaluate aspects of the wound healing process and growth factors production of vascular endothelial growth factors (VEGF) as well a necrosis reduction in the skin flap of rat models. Moreover, phenytoin (PHT) and cyclosporine A (CsA) were used topically as positive control treatments in wound and necrosis models, respectively. Immunohistochemistry (IHC) VEGF, transforming growth factor-β (TGF-β), fibroblast growth factor (FGF) and histopathology were analysed on the wounds of rats...
April 2024: Clinical and Experimental Pharmacology & Physiology
https://read.qxmd.com/read/38407373/immune-profiling-of-vulvar-squamous-cell-cancer-discovers-a-macrophage-rich-subtype-associated-with-poor-prognosis
#36
JOURNAL ARTICLE
Mateja Condic, Andrea Rohr, Soheila Riemann, Christian Staerk, Tiyasha H Ayub, Anna Doeser, Thomas Zillinger, Sabine Merkelbach-Bruse, Reinhard Buettner, Winfried Barchet, Christian Rudlowski, Alexander Mustea, Kirsten Kübler
The incidence rates of vulvar squamous cell cancer (VSCC) have increased over the past decades, requiring personalized oncologic approaches. Currently, lymph node involvement is a key factor in determining prognosis and treatment options. However, there is a need for additional immune-related biomarkers to provide more precise treatment and prognostic information. Here, we used immunohistochemistry and expression data to characterize immune cells and their spatial distribution in VSCC. Hierarchical clustering analysis identified distinct immune subtypes, of which the macrophage-rich subtype was associated with adverse outcome...
February 26, 2024: Cancer Res Commun
https://read.qxmd.com/read/38396128/correlation-of-mri-quantitative-perfusion-parameters-with-egfr-vegf-and-egfr-gene-mutations-in-non-small-cell-cancer
#37
JOURNAL ARTICLE
Mingyue Zou, Bingqian Zhang, Lei Shi, Haijia Mao, Yanan Huang, Zhenhua Zhao
To explore the relationship between quantitative perfusion histogram parameters of dynamic contrast enhanced magnetic resonance imaging (DCE-MRI) with the expression of tumor tissue epidermal growth factor receptor (EGFR), vascular endothelial growth factor (VEGF) and EGFR gene mutations in non-small cell lung cancer (NSCLC). A total of 44 consecutive patients with known NSCLC were recruited from March 2018 to August 2021. Histogram parameters (mean, uniformity, skewness, energy, kurtosis, entropy, percentile) of each (Ktrans , Kep , Ve , Vp , Fp ) were obtained by Omni Kinetics software...
February 23, 2024: Scientific Reports
https://read.qxmd.com/read/38396011/exosomes-of-endothelial-progenitor-cells-repair-injured-vascular-endothelial-cells-through-the-bcl2-bax-caspase-3-pathway
#38
JOURNAL ARTICLE
Wei Tan, Yanling Li, Lu Ma, Xinying Fu, Qingyin Long, Fanchen Yan, Wanyu Li, Xiaodan Liu, Huang Ding, Yang Wang, Wei Zhang
The main objective of this study is to evaluate the influence of exosomes derived from endothelial progenitor cells (EPC-Exo) on neointimal formation induced by balloon injury in rats. Furthermore, the study aims to investigate the potential of EPC-Exo to promote proliferation, migration, and anti-apoptotic effects of vascular endothelial cells (VECs) in vitro. The underlying mechanisms responsible for these observed effects will also be thoroughly explored and analyzed. Endothelial progenitor cells (EPCs) was isolated aseptically from Sprague-Dawley (SD) rats and cultured in complete medium...
February 23, 2024: Scientific Reports
https://read.qxmd.com/read/38394403/angioleiomyoma-of-the-uterus-presenting-as-an-endometrial-polyp-an-uncommon-occurrence-of-two-cases-with-a-literature-review
#39
JOURNAL ARTICLE
Vamsi K Kapaganti, Mohammed Shahin, Anurag Mishra, Pruthiviraj Sethi, Saubhagya K Jena, Bhismadev Chhatria, Pavithra Ayyanar
Angioleiomyoma is a benign neoplasm that arises from vascular smooth muscle cells. Angioleiomyoma of the endometrium is very uncommon. The differential diagnoses of this entity are myopericytoma, angiomyofibroblastoma, endometrial stromal tumor, and perivascular epithelioid cell tumor. 31-year-old and 45-year-old patients presented with heavy menstrual bleeding, lower abdomen pain, and dysmenorrhea. Perspeculum and radiological investigations showed an endometrial polyp. They underwent diagnostic hysteroscopy, polypectomy, and endometrial biopsy...
November 9, 2023: Indian Journal of Pathology & Microbiology
https://read.qxmd.com/read/38391317/multifocal-intraosseous-pseudomyogenic-epithelioid-sarcoma-like-hemangioendothelioma-a-rare-presentation-of-an-uncommon-tumor
#40
JOURNAL ARTICLE
Vishwapriya M Godkhindi, Vidya Monappa, Sharada Mailankody, Umesh Velu, Shuiab M V Mohammed, Aisharya Banerjee
Pseudomyogenic hemangioendothelioma (PHE) is an uncommon mesenchymal neoplasm of intermediate malignant potential showing endothelial differentiation. Around 20 cases of primary osseous PHE have been reported to date. A 16-year-old boy presented with complaints of pain in his right leg. Imaging revealed multifocal intramedullary and cortical-based lytic lesions involving long and small bones. Microscopic examination revealed plump, spindled cells arranged in fascicles and admixed "epithelioid" and "rhabdoid" cells sans vasoformative areas...
July 26, 2023: Indian Journal of Pathology & Microbiology
keyword
keyword
168306
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.