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Endothelial immunohistochemistry

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https://www.readbyqxmd.com/read/30536712/modeling-and-multi-scale-characterization-of-the-quantitative-imaging-based-fibrosis-index-reveals-pathophysiological-transcriptome-and-proteomic-correlates-of-lung-fibrosis-induced-by-fractionated-irradiation
#1
Cheng Zhou, Mahmoud R Moustafa, Liji Cao, Mark Kriegsmann, Martin Winter, Christian Schwager, Bleddyn Jones, Shijun Wang, Tobias Bäuerle, Ping-Kun Zhou, Martina Schnölzer, Wilko Weichert, Juergen Debus, Amir Abdollahi
Pulmonary fibrosis represents a leading cause of morbidity and mortality worldwide. Therapy induced lung fibrosis constitutes a pivotal dose-limiting side effect of radiotherapy and other anticancer agents. We aimed to develop objective criteria for assessment of fibrosis and discover pathophysiological and molecular correlates of lung fibrosis as a function of fractionated whole thoracic irradiation. Dose-response series of fractionated irradiation was utilized to develop a non-invasive and quantitative measure for the degree of fibrosis - the fibrosis index (FI)...
December 11, 2018: International Journal of Cancer. Journal International du Cancer
https://www.readbyqxmd.com/read/30531686/hypoxia-inducible-factor-1-alpha-hif-1%C3%AE-vascular-endothelial-growth-factor-vegf-pathway-participates-in-angiogenesis-of-myocardial-infarction-in-muscone-treated-mice-preliminary-study
#2
Yingqiang Du, Yingbin Ge, Zhihui Xu, Nan Aa, Xin Gu, Haoyu Meng, Zhou Lin, Dongxiao Zhu, Jingjing Shi, Ruijuan Zhuang, Xueming Wu, Xiaoyan Wang, Zhijian Yang
BACKGROUND Angiogenesis plays a crucial role in myocardial infarction (MI) treatment by ameliorating myocardial remodeling, thus improving cardiac function and preventing heart failure. Muscone has been reported to have beneficial effects on cardiac remodeling in MI mice. However, the effects of muscone on angiogenesis in MI mice and its underlying mechanisms remain unknown. MATERIAL AND METHODS Mice were randomly divided into sham, MI, and MI+muscone groups. The MI mouse model was established by ligating the left anterior descending coronary artery...
December 8, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/30527680/thrombin-promotes-pericyte-coverage-by-tie2-activation-in-a-rat-model-of-intracerebral-hemorrhage
#3
En Hu, Wang Hu, Ali Yang, Huajun Zhou, Jun Zhou, Jiekun Luo, Yang Wang, Tao Tang, Hanjin Cui
Pericyte coverage on the endothelial tubes leads to the formation of a mature and stable microvessel system, which is critical for brain repair after intracerebral hemorrhage (ICH). We report herein that thrombin promotes pericyte coverage by activating Tie2 and the downstream signaling pathway PI3K/Akt in a rat model of ICH. ICH was induced by injection of autologous blood with or without thrombin inhibitor hirudin. Rats were treated with thrombin alone or in combination with a Tie2 inhibitor. The expression of total- and phospho-Tie2, PI3K and phospho-Akt, blood perfusion, pericyte coverage, IgG extravasation, neuron survival and neurological deficits were evaluated by western blot, fluorescein-5-isothiocyanate-dextran staining, immunohistochemistry, Nissl staining and modified neurological severity scores respectively...
December 5, 2018: Brain Research
https://www.readbyqxmd.com/read/30527465/citrullinated-histone-h3-early-biomarker-of-neutrophil-extracellular-traps-in-septic-liver-damage
#4
Kozo Nomura, Tomoharu Miyashita, Yasuhiko Yamamoto, Seiichi Munesue, Ai Harashima, Hideo Takayama, Sachio Fushida, Tetsuo Ohta
BACKGROUND: Neutrophil extracellular traps (NETs) play a crucial role in host defense, but excess and prolonged interaction of NETs with platelets can cause severe inflammation and host organ damage. Modification of histone H3 by citrullination is involved in in vitro NET formation. The phosphodiesterase III inhibitor, cilostazol (Ciz), which has a protective effect on liver sinusoidal endothelial cells and inhibits platelet aggregation, may prevent organ damage caused by excess NETosis...
February 2019: Journal of Surgical Research
https://www.readbyqxmd.com/read/30524369/vascular-endothelial-growth-factor-vegf-induced-downstream-responses-to-transient-receptor-potential-vanilloid-1-trpv1-and-3-iodothyronamine-3-t-1-am-in-human-corneal-keratocytes
#5
Ersal Türker, Fabian Garreis, Noushafarin Khajavi, Peter S Reinach, Pooja Joshi, Tobias Brockmann, Alexander Lucius, Nina Ljubojevic, Elizabeth Turan, Drew Cooper, Felix Schick, Rob Reinholz, Uwe Pleyer, Josef Köhrle, Stefan Mergler
This study was undertaken to determine if crosstalk among the transient receptor potential (TRP) melastatin 8 (TRPM8), TRP vanilloid 1 (TRPV1), and vascular endothelial growth factor (VEGF) receptor triad modulates VEGF-induced Ca2+ signaling in human corneal keratocytes. Using RT-PCR, qPCR and immunohistochemistry, we determined TRPV1 and TRPM8 gene and protein coexpression in a human corneal keratocyte cell line (HCK) and human corneal cross sections. Fluorescence Ca2+ imaging using both a photomultiplier and a single cell digital imaging system as well as planar patch-clamping measured relative intracellular Ca2+ levels and underlying whole-cell currents...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/30523755/tgf-%C3%AE-secreted-by-tumor-associated-macrophages-promotes-proliferation-and-invasion-of-colorectal-cancer-via-mir-34a-vegf-axis
#6
Danhua Zhang, Xinguang Qiu, Jianhua Li, Shouhua Zheng, Liwen Li, Hongchao Zhao
Tumor-associated macrophages (TAMs) were reported to be involved in colorectal cancer (CRC) progression. However, its biological role and underlying mechanism in CRC remained to be elucidated. In this study, the expressions of the macrophage marker CD68 and transforming growth factor β1 (TGF-β1) in CRC tumor tissues and adjacent tissues were detected by immunohistochemistry. The expression levels of miR-34a, TGF-β1 and vascular endothelial growth factor (VEGF) in CRC tumor tissues and peripheral blood macrophages were measured by quantitative real-time PCR (qRT-PCR) and western blot...
December 7, 2018: Cell Cycle
https://www.readbyqxmd.com/read/30515388/early-enhanced-leucine-rich-%C3%AE-2-glycoprotein-1-expression-in-glomerular-endothelial-cells-of-type-2-diabetic-nephropathy-model-mice
#7
Sona Haku, Hiromichi Wakui, Kengo Azushima, Kotaro Haruhara, Sho Kinguchi, Kohji Ohki, Kazushi Uneda, Ryu Kobayashi, Miyuki Matsuda, Takahiro Yamaji, Takayuki Yamada, Shintaro Minegishi, Tomoaki Ishigami, Akio Yamashita, Kenichi Ohashi, Kouichi Tamura
Abnormal angiogenesis plays a major role in the development of early stage diabetic nephropathy. Vascular endothelial growth factor (VEGF) is a classical proangiogenic factor that regulates abnormal glomerular angiogenesis linked to glomerular hypertrophy in the early stage of diabetic nephropathy. Leucine-rich α -2-glycoprotein-1 (LRG1) was recently reported as a novel proangiogenic factor that is expressed in endothelial cells and promotes angiogenesis by modulating the transforming growth factor- β signaling pathway...
2018: BioMed Research International
https://www.readbyqxmd.com/read/30515094/paeonol-attenuated-inflammatory-response-of-endothelial-cells-via-stimulating-monocytes-derived-exosomal-microrna-223
#8
Yarong Liu, Chao Li, Hongfei Wu, Xianmei Xie, Ying Sun, Min Dai
Introduction: Paeonol, an active compound isolated from the radix of Cortex Moutan , has been shown to have anti-atherosclerosis effects by regulating blood cells' function and protecting vascular cells injury. Besides, emerging evidences has proven that exosomes might play a pivotal role in intercellular communication by transmiting proteins and microRNAs from cell to cell. However, the relationship between monocytes-derived exosomal microRNA-223 and vascular inflammation injury along with paeonol' effects are still not clear...
2018: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/30514679/-establishment-of-a-rat-model-of-dimethylbenzanthracene-induced-vulvar-squamous-intraepithelial-lesions
#9
Yijin Fan, Huajun Tang, Yao Liu, Chengzhi Li
OBJECTIVE: To establish a SD rat model of vulvar squamous intraepithelial lesions. METHODS: Seventy female SD rats were randomized into 4 groups, namely the blank control group ( n =10), mechanical irritation group ( n =10), acetone solution group ( n =10), and mechanical irritation with DMBA acetone solution group ( n =40, model group), and the corresponding treatments were administered 3 times a week for 14 weeks. The changes of the vulvar skin of the rats were observed regularly until the 18th week...
November 30, 2018: Nan Fang Yi Ke da Xue Xue Bao, Journal of Southern Medical University
https://www.readbyqxmd.com/read/30511851/pretargeting-and-bioorthogonal-click-chemistry-mediated-endogenous-stem-cell-homing-for-heart-repair
#10
Zhenhua Li, Deliang Shen, Shiqi Hu, Teng Su, Ke Huang, Feiran Liu, Lei Hou, Ke Cheng
Stem cell therapy is one of the promising strategies for the treatment of ischemic heart disease. However, the clinical application of stem cells transplantation is limited by low cell engraftment in the infarcted myocardium. Taking advantage of pretargeting and bioorthogonal chemistry, we engineered a pretargeting and bioorthogonal chemistry (PTBC) system to capture endogenous circulating stem cells and target them to the injured heart for effective repair. Two bioorthogonal antibodies were i.v. administrated with a pretargeting interval (48 h)...
December 4, 2018: ACS Nano
https://www.readbyqxmd.com/read/30511620/melatonin-protects-against-streptozotocin-induced-diabetic-cardiomyopathy-by-the-phosphorylation-of-vascular-endothelial-growth-factor-a-vegf-a
#11
Yasemin Behram Kandemir, Ünal Guntekin, Veysel Tosun, Necmettin Korucuk, Mehmet Nuri Bozdemir
The aim of the present study is to investigate if the melatonin has any protective effect on diabetic cardiomyopathy and antioxidant enzymes via phosphorylation of vascular endothelial growth factor-A (VEGF-A). A total of 40 male Wistar rats were enrolled in the study. Rats were divided into four groups: group 1 (control, n=10), group 2 (DM, n=10), group 3 (melatonin, n=10), and group 4 (melatonin+DM, n=10). Melatonin was injected intraperitoneally at a dose of 50 mg/kg/day for 56 days to group 3 and group 4...
November 30, 2018: Cellular and Molecular Biology
https://www.readbyqxmd.com/read/30510453/fpr1-mediates-the-tumorigenicity-of-human-cervical-cancer-cells
#12
Guangming Cao, Zhenyu Zhang
Purpose: The present study aimed to investigate the role of FPR1 and the downstream effectors such as NF-κB and IL-6/8 in the development of cervical cancer. Patients and methods: FPR1 protein expression was detected via immunohistochemical staining in tissue microarrays containing cervical cancer tissues from 185 patients. Following FPR1 silencing in SiHa cells using lentiviral siRNA delivery, biological characteristics and tumor formation were evaluated in vitro and in vivo, respectively...
2018: Cancer Management and Research
https://www.readbyqxmd.com/read/30510403/valproic-acid-enhances-the-viability-of-random-pattern-skin-flaps-involvement-of-enhancing-angiogenesis-and-inhibiting-oxidative-stress-and-apoptosis
#13
Hongqiang Wu, Jian Ding, Lei Wang, Jinti Lin, Shihen Li, Guangheng Xiang, Liangfu Jiang, Huazi Xu, Weiyang Gao, Kailiang Zhou
Background: Random skin flaps are commonly applied during plastic surgery, but distal flap necrosis limits their clinical applications. Valproic acid (VPA), a histone deacetylase inhibitor and a traditional antiepileptic agent, may promote flap survival. Materials and methods: Sprague-Dawley rats were randomly divided into VPA-treated and control groups. All rats received VPA or saline by intraperitoneal injections once daily for 7 days after the modified McFarlane flap model was established...
2018: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/30504718/morc2-enhances-tumor-growth-by-promoting-angiogenesis-and-tumor-associated-macrophage-recruitment-via-wnt-%C3%AE-catenin-in-lung-cancer
#14
Meihan Liu, Xiaochun Sun, Shaomin Shi
BACKGROUND/AIMS: In this study, we aimed to investigate how MORC family CW-type zinc finger 2 (MORC2) affects tumor progression of lung cancer. METHODS: The MORC2 level was analyzed by real-time RT-PCR and immunohistochemistry (IHC) in normal control tissues and lung cancers. LL/2 cells overexpressing MORC2 were used to study how MORC2 expression influences lung cancer progression. The effects of MORC2 on cell viability, migration and invasion were assessed by MTT assay, Western blotting, and transwell assays, respectively...
November 30, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/30502286/plasma-ameliorates-endothelial-dysfunction-in-burn-injury
#15
Mariana Vigiola Cruz, Bonnie C Carney, Jenna N Luker, Kyle W Monger, Juan Sebastian Vazquez, Lauren T Moffatt, Laura S Johnson, Jeffrey W Shupp
BACKGROUND: A complex inflammatory response mediates the systemic effects of burn shock. Disruption of the endothelial glycocalyx causes shedding of structural glycoproteins, primarily syndecan-1 (SDC-1), leading to endothelial dysfunction. These effects may be mitigated by resuscitative interventions. MATERIALS AND METHODS: Sprague-Dawley rats were used to create small, medium, and large burns and uninjured controls. Three different intravenous resuscitation protocols were applied within each group: Lactated Ringer's (LR) alone, LR plus fresh frozen plasma (FFP), or LR plus albumin...
January 2019: Journal of Surgical Research
https://www.readbyqxmd.com/read/30497478/oral-lichen-planus-associated-fibroblasts-acquire-myofibroblast-characteristics-and-secrete-pro-inflammatory-cytokines-in-response-to-porphyromonas-gingivalis-lipopolysaccharide-stimulation
#16
Liping Wang, Yinshen Yang, Xiaoqin Xiong, Ting Yu, Xinhong Wang, Wenxia Meng, Haiyan Wang, Gang Luo, Linhu Ge
BACKGROUND: Oral lichen planus (OLP) is a chronic inflammatory oral mucosal disease in which comprehensive inflammation-related cytokines are involved. These cytokines are commonly produced by immune cells and specific nonimmune cells including keratinocytes, endothelial cells and fibroblasts. This raises the question of whether fibroblasts in OLP lesions contribute to the inflammatory process upon inflammatory simulation. METHODS: Primary cultured Oral lichen-planus-associated fibroblasts (OLP AFs, n = 5) and normal buccal mucosal fibroblasts (NFs, n = 5) were examined by immunohistochemistry, Western blotting and reverse transcription-polymerase chain reactions (RT-PCR)...
November 29, 2018: BMC Oral Health
https://www.readbyqxmd.com/read/30497276/inflammatory-smooth-muscle-cells-induce-endothelial-cell-alterations-to-influence-cerebral-aneurysm-progression-via-regulation-of-integrin-and-vegf-expression
#17
Peixi Liu, Yuan Shi, Zhiyuan Fan, Yingjie Zhou, Yaying Song, Yingjun Liu, Guo Yu, Qingzhu An, Wei Zhu
Cerebral aneurysm growth is characterized by vessel wall frailness, although the underlying cellular mechanisms are unclear. Here, we examined the relationship between inflammatory smooth muscle cells (SMCs) and endothelial cells (ECs) in cerebral aneurysms, including the mechanisms underlying inflammatory SMC-induced changes in ECs. Five saccular cerebral aneurysms were collected and five temporal artery samples were used as controls. Cells and cytokines were detected by immunohistochemistry and TUNEL (transferase dUTP nick end labeling) assays performed to evaluate apoptosis...
November 30, 2018: Cell Transplantation
https://www.readbyqxmd.com/read/30483777/ubiad1-expression-is-associated-with-cardiac-hypertrophy-in-spontaneously-hypertensive-rats
#18
Bingju Yan, Jun Wang
The present study investigated the potential role of UbiA prenyltransferase domain-containing 1 (UBIAD1) in the pathogenesis of hypertensive cardiac hypertrophy. Spontaneously hypertensive rats (SHRs) and Wistar‑Kyoto (WKY) rats at 8, 16 and 28 weeks of age were used. Blood pressure was measured using a non‑invasive tail cut‑off system. Cardiac functional index was assessed by arterial catheterization. Myocardial structure and cell apoptosis were evaluated by hematoxylin and eosin staining, and terminal deoxynucleotidyl‑transferase‑mediated dUTP nick end labeling assays, respectively...
November 26, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/30478679/isolation-and-characterization-of-cells-derived-from-human-epithelial-rests-of-malassez
#19
Kayoko Kitajima, Ridhima Das, Xiao Liang, Evelyn Neppelberg, Anne Christine Johannessen, Daniela Elena Costea, Masaru Igarashi
The epithelial rests of Malassez (ERMs) might represent a valuable source of oral epithelial cells with stem cell properties. The purpose of this study was to isolate and characterize cells derived from human ERM, and compare them with cells derived from matched normal oral mucosa (NOM). Matched tissue specimens of the periodontal ligament of extracted tooth and NOM were collected. Cells were isolated in culture, then characterized by immunohistochemistry and flow cytometry for expression of pancytokeratin, ESA, PDGFRB, CD31 and CD44...
November 26, 2018: Odontology
https://www.readbyqxmd.com/read/30474335/pre-pubertal-treatment-with-a-gnrh-agonist-in-bitches-effect-on-the-uterus-and-hormone-receptor-expression
#20
Sabine Schäfer-Somi, Duygu Kaya, Mahmut Sözmen, Semra Kaya, Selim Aslan
Aim of the study was to examine the effect of deslorelin on uterine tissues of eleven pre-pubertal bitches aged 4.2 ± 0.6 m. Implants containing placebo (sodium chloride 0.9%; n = 4, G I), 4.7 mg (n = 3, GII) or 9.4 mg (n = 4, GIII) deslorelin acetate (Suprelorin® ; Virbac, France), were administered subcutaneously. Signs of oestrus, vaginal cytology, serum progesterone (P4) and estradiol-17β (E2) concentrations were monitored until the occurrence of oestrus. Bitches were ovariohysterectomized and sections from the uterine tissue were subjected to immunohistochemistry (IHC) for detection of GnRH receptor (R), Kisspeptin (KP)10, Kisspeptin receptor (GPR54), androgen receptor (AR), oestrogen receptor (ER) α,β, and progesterone receptor (PR)...
November 2018: Reproduction in Domestic Animals, Zuchthygiene
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