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Endothelial immunohistochemistry

Juciano Gasparotto, Camila Tiefensee Ribeiro, Helen Tais da Rosa-Silva, Rafael Calixto Bortolin, Thallita Kelly Rabelo, Daniel Oppermann Peixoto, José Claudio Fonseca Moreira, Daniel Pens Gelain
The receptor for advanced glycation endproducts (RAGE) is a transmembrane, immunoglobulin-like receptor that interacts with a broad repertoire of extracellular ligands. RAGE belongs to a family of cell adhesion molecules and is considered a key receptor in the inflammation axis and a potential contributor to the neurodegeneration. The present study aimed to investigate the content and cell localization of RAGE in the brain of Wistar rats subjected to systemic inflammation induced by a single dose of lipopolysaccharide (LPS, 5 mg/kg, i...
August 9, 2018: Molecular Neurobiology
Huan Qian, Zhangqi Dou, Wu Ruan, Pingyou He, John H Zhang, Feng Yan
Studies have suggested that blood-brain barrier (BBB) disruption contributes to the pathogenesis of early brain injury after subarachnoid haemorrhage (SAH). Activation of the receptor tyrosine kinase ErbB4 can cause intramembrane proteolysis and release a soluble intracellular domain (ICD) that modulates transcription in the nucleus. This study was carried out to investigate the potential roles of ErbB4 in preserving BBB integrity after experimental SAH, as well as the underlying mechanisms of its protective effects...
2018: Frontiers in Neuroscience
Daina Kashiwazaki, Shusuke Yamamoto, Naoki Akioka, Naoya Kuwayama, Kyo Noguchi, Satoshi Kuroda
BACKGROUND AND PURPOSE: This study was aimed to assess the hypothesis that unstable plaque formation in the carotid artery is one of phenotypes of chronic and systemic inflammation. METHODS: This study included 8 symptomatic patients with internal carotid stenosis (ICS) and 7 healthy controls. All subjects underwent 18 F-fluorodeoxyglucos positron emission tomography (18 F-FDG PET) of whole body. Plaque vulnerability was evaluated on magnetic resonance imaging (MRI)...
August 4, 2018: Journal of Stroke and Cerebrovascular Diseases: the Official Journal of National Stroke Association
Patrick Süß, Florian Volz, Christine Lang, Ori Staszewksi, Gabriele Palmedo, Christian A Taschner, Christian Scheiwe, Philipp Kurz, Thomas Mentzel, Marco Prinz
Epithelioid hemangioendothelioma (EHE) is a rare vascular neoplasm predominantly occurring in the soft tissue. A majority of EHE cases is driven by a WW domain containing transcription regulator protein 1 (WWTR1)-calmodulin-binding transcription activator 1 (CAMTA1) gene fusion. The clinical course of EHE ranges from long-term favorable to rapidly aggressive. Few cases of intracranial EHE have been reported, none of which has been molecularly proven. We report a case of left parietal meningeal EHE, which was resected 15 years after initial radiological detection...
July 10, 2018: Journal of Neuropathology and Experimental Neurology
Leona W Ayers, Arturo Barbachano-Guerrero, Shane C McAllister, Julie A Ritchie, Elizabeth Asiago-Reddy, Linda C Bartlett, Ethel Cesarman, Dongliang Wang, Rosemary Rochford, Jeffrey N Martin, Christine A King
PURPOSE: Kaposi sarcoma (KS) is a vascular tumor initiated by infection of endothelial cells (ECs) with KS-associated herpesvirus (KSHV). KS is dependent on sustained pro-inflammatory signals provided by intra-lesional leukocytes and continued infection of new ECs. However, the sources of these cytokines and infectious virus within lesions are not fully understood. Here, mast cells (MCs) are identified as pro-inflammatory cells within KS lesions that are permissive for, and activated by, infection with KSHV...
July 3, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Ting Xiao, Qiang Zhang, Shumin Zong, Wei-Long Zhong, Yuan Qin, Zhun Bi, Shuang Chen, Hui-Juan Liu, Jun-Jie Wei, Bi-Jiao Zhou, Lu-Meng Wang, Hong-Gang Zhou, Yan-Rong Liu, Tao Sun, Cheng Yang
BACKGROUND: Tumor cells transfer into endothelial cells by epithelial-endothelial transition (EET), which is characterized by vasculagenic mimicry (VM) in morphology. VM can change tumor microcirculation, progression, and metastasis. However, the molecular mechanisms of endothelial-like transition remain unclear. EET is a subtype of epithelial-mesenchymal transition (EMT). Twist1, a transcriptional regulatory factor of EMT, is an important factor that induces EET in hepatocellular carcinoma(HCC), but the upstream signal of Twist1 is unclear...
August 6, 2018: Journal of Experimental & Clinical Cancer Research: CR
Bin Leng, Futian Tang, Meili Lu, Zhen Zhang, Hongxin Wang, Yingjie Zhang
AIMS: Astragaloside IV (As-IV) is the major active ingredient of Astragalus membranaceus and has diverse pharmacological activities, including anti-inflammatory and antioxidant effects. However, the beneficial effect of As-IV on protecting vascular endothelial dysfunction is not completely understood. The aim of this study was to investigate the protective effect and mechanism of As-IV on vascular endothelial dysfunction. MATERIALS AND METHODS: A diabetes model was established by intraperitoneal injection of streptozotocin (STZ)...
August 3, 2018: Life Sciences
Kana Hoshino-Negishi, Masayoshi Ohkuro, Tomoya Nakatani, Yoshikazu Kuboi, Miyuki Nishimura, Yoko Ida, Jungo Kakuta, Akiko Hamaguchi, Minoru Kumai, Tsutomu Kamisako, Fumihiro Sugiyama, Wataru Ikeda, Naoto Ishii, Nobuyuki Yasuda, Toshio Imai
OBJECTIVE: To elucidate the role of the fractalkine (FKN)-CX3CR1 pathway in joint destruction in rheumatoid arthritis, we examined the effect of treatment with anti-mouse FKN (mFKN) monoclonal antibody (mAb) on joint destruction and the migration of osteoclast precursors (OCPs) into the joint, using the collagen-induced arthritis (CIA) model. METHODS: DBA/1 mice were immunized with bovine type II collagen to induce arthritis, then treated with anti-mFKN mAb. Disease severity was monitored by arthritis score, and joint destruction was evaluated by soft X-ray and histological analysis...
August 6, 2018: Arthritis & Rheumatology
Douglas G Walker, Tiffany M Tang, Lih-Fen Lue
The focus of this study is the expression of Toll-like receptor-3 (TLR-3), a receptor for double-stranded RNA, in human brains affected by Alzheimer's disease (AD) pathology. Toll-like receptors are a family of pattern recognition molecules primarily involved in host defenses to microbial pathogens, but roles in neurodegenerative disease have also been shown, as amyloid beta (Aβ) can be a ligand for TLR-2 and -4 and α-synuclein for TLR-1 and TLR-2, while TLR-9 activation promotes Aβ removal. However, involvement of TLR-3 in AD has not been rigorously studied...
August 1, 2018: Experimental Neurology
Min Bi, Jiawei Wang, Yidan Zhang, Longzhu Li, Linhui Wang, Ran Yao, Shijie Duan, Suijun Tong, Jianpeng Li
Bone marrow mesenchymal stem cells (BMSCs) are used as a great promising choice for the treatment of cerebral ischemia. Herein, we discuss the neuroprotective effects of the combination of BMSCs transplantation and mild hypothermia (MH) in an ischemia-reperfusion rat model. First, BMSCs were isolated using density gradient centrifugation and the adherent screening method, followed by culture, identification and labeling with DAPI. Second, adult male SD rats were divided into 5 groups: sham group (surgery without blockage of middle cerebral artery), model group (middle cerebral artery occlusion (MCAO) was established 2h prior to reperfusion), BMSCs group (injection of BMSCs via the lateral ventricle 24h after MCAO), MH group (mild hypothermia for 3h immediately after MCAO) and combination therapy group (combination of BMSCs and MH)...
2018: PloS One
Ingiridur Skirnisdottir, Helena Åkerud, Tomas Seidal
The aim of the present retrospective cohort study was to investigate the prognostic effect of epidermal growth factor receptor (EGFR) and the angiogenesis regulator vascular endothelial growth factor receptor 2 (VEGF‑R2) on disease-free survival (DFS) rate and recurrent disease, and their association with clinicopathological characteristics in 131 patients with International Federation of Gynecology and Obstetrics (FIGO) stages I-II epithelial ovarian cancer. The techniques of tissue microar-rays and immunohistochemistry were used for the positive detection of the markers...
July 31, 2018: International Journal of Oncology
Haidy El-Azzamy, Svetlana V Dambaeva, Dimantha Katukurundage, Maria D Salazar Garcia, Annie Skariah, Youssef Hussein, Alfredo Germain, Emilio Fernandez, Alice Gilman-Sachs, Kenneth D Beaman, Joanne Kwak-Kim
PROBLEM: Angiogenesis and vascular remodeling in secretory endometrium represent one of the crucial steps in pregnancy establishment, for which uterine NK (uNK) cells have an important role. Impairment of these steps may proceed to implantation and instigate initial pathology of recurrent pregnancy losses (RPL). In this study, we aim to investigate vascular development and density of uNK cells in secretory endometrium of women with RPL. METHODS OF STUDY: Mid-secretory phase endometrial tissues from women with RPL (n = 15) and fertile controls (n = 7) were investigated...
July 31, 2018: American Journal of Reproductive Immunology: AJRI
Ying Zhuge, Manuela M Regueiro, Runxia Tian, Yan Li, Xiaomei Xia, Roberto Vazquez-Padron, Sharon Elliot, Seth R Thaller, Zhao-Jun Liu, Omaida C Velazquez
OBJECTIVE: Endothelial progenitor cells (EPCs) are the key cells of postnatal neovascularization, and mesenchymal stem cells (MSCs) possess pluripotent differentiation capacity and contribute to tissue regeneration and wound healing. Both EPCs and MSCs are critical to the wound repair process, which is hindered in diabetes mellitus. Diabetes has been shown to decrease the function of these progenitor cells, whereas estrogen has beneficial wound healing effects. However, the role of estrogen in modulating EPC and MSC biology in diabetes is unknown...
July 28, 2018: Journal of Vascular Surgery
Anna Goussia, Nafsika Simou, Flora Zagouri, Kyriaki Manousou, Georgios Lazaridis, Helen Gogas, Angelos Koutras, Maria Sotiropoulou, George Pentheroudakis, Dimitrios Bafaloukos, Christos Markopoulos, Helen Patsea, Christos Christodoulou, Pavlos Papakostas, Thomas Zaramboukas, Epaminontas Samantas, Paris Kosmidis, Vasileios Venizelos, Charisios Karanikiotis, George Papatsibas, Grigorios Xepapadakis, Konstantine T Kalogeras, Christina Bamia, Meletios-Athanassios Dimopoulos, Vassiliki Malamou-Mitsi, George Fountzilas, Anna Batistatou
Several studies support an important role of angiogenesis in breast cancer growth and metastasis. The main objectives of the study were to investigate the immunohistochemical expression of vascular endothelial growth factor (VEGF) family ligands (VEGF-A and VEGF-C) and receptors (VEGFR1, VEGFR2 and VEGFR3) in breast cancer and their associations with clinicopathological parameters, cancer subtypes/subgroups and patient outcome. Formalin-fixed paraffin-embedded tumor tissue samples were collected from early-stage breast cancer patients treated with anthracycline-based chemotherapy within a randomized trial...
2018: PloS One
A Anjaneya, K P Singh, S Cherian, M Saminathan, R Singh, M A Ramakrishnan, S Maan, N S Maan, D Hemadri, P P Rao, K Putty, Y Krishnajyothi, P P Mertens
Bluetongue virus (BTV) is neurotropic in nature, especially in ruminant fetuses and in-utero infection results in abortion and congenital brain malformations. The aim of the present study was to compare the neuropathogenicity of major Indian BTV serotypes 1, 2, 10, 16 and 23 by gross and histopathological lesions and virus distribution in experimentally infected neonatal BALB/c mice. Each BTV serotype (20 μl of inoculum containing 1 × 105 tissue culture infectious dose [TCID]50 /ml of virus) was inoculated intracerebrally into 3-day-old mice, while a control group was inoculated with mock-infected cell culture medium...
July 2018: Journal of Comparative Pathology
Ewa Kaniewska-Bednarczuk, Barbara Kutryb-Zajac, Padmini Sarathchandra, Iwona Pelikant-Malecka, Alicja Sielicka, Izabela Piotrowska, Ewa M Slominska, Adrian H Chester, Magdi H Yacoub, Ryszard T Smolenski
BACKGROUND: The calcific aortic valve disease (CAVD) is a common heart pathology that involves inflammation, fibrosis, and calcification of aortic valve leaflets. All these processes could be affected by changes in the extracellular purinergic signaling that depend on the activity of ectonucleotidases, mainly ectonucleoside triphosphate diphosphohydrolase 1 (CD39, eNTPD1) and ecto-5'nucleotidase (CD73, e5NT). OBJECTIVE AND METHODS: We investigated the localization of CD39 and CD73 proteins in human noncalcified and calcified aortic valves using immunohistochemistry together with analysis of NTPDases and e5NT activities in aortic valve homogenates by analysis of substrate into product conversion by high-performance liquid chromatography...
June 7, 2018: Cardiovascular Pathology: the Official Journal of the Society for Cardiovascular Pathology
Steven E Trasino, Xiao-Han Tang, Maria M Shevchuk, Mary E Choi, Lorraine J Gudas
Vitamin A (VA) and its derivatives, known as retinoids, play critical roles in renal development through retinoic acid receptor β2 (RARβ2). Disruptions in VA signaling pathways are associated with the onset of diabetic nephropathy (DN). Despite the known role of RARβ2 in renal development, the effects of selective agonists for RARβ2 in a high fat diet (HFD) model of DN are unknown. Here we examined whether AC261066 (AC261), a highly selective agonist for RARβ2, exhibited therapeutic effects in a HFD model of DN in C57BL/6 mice...
July 27, 2018: Journal of Pharmacology and Experimental Therapeutics
Haruhiko Yamazaki, Tomoyuki Yokose, Hiroyuki Hayashi, Hiroyuki Iwasaki, Sachie Osanai, Nobuyasu Suganuma, Hirotaka Nakayama, Katsuhiko Masudo, Yasushi Rino, Munetaka Masuda
PURPOSE: Angiogenesis plays a crucial role in the development, growth, and metastasis of carcinomas, and studies have reported conflicting evidence regarding the VEGFR expression in anaplastic thyroid cancer. We investigated the expression of VEGFR2 in patients with anaplastic thyroid cancer (ATC) and analyzed the clinical response to the VEGFR inhibitor lenvatinib. METHODS: This cross-sectional study included primary tumor samples obtained from 12 patients with ATC, including 5 males and 7 females (age range 63-89 years) who underwent surgery or core needle biopsy for a thyroid tumor in the Department of Breast and Endocrine Surgery at Kanagawa Cancer Center in Kanagawa, Japan...
July 26, 2018: Cancer Chemotherapy and Pharmacology
Lourdes Acosta, Sara Morcuende, Silvia Silva-Hucha, Angel M Pastor, Rosa R de la Cruz
Vascular endothelial growth factor (VEGF) was initially characterized by its activity on the vascular system. However, there is growing evidence indicating that VEGF also acts as a neuroprotective factor, and that its administration to neurons suffering from trauma or disease is able to rescue them from cell death. We questioned whether VEGF could also maintain damaged neurons in a neurotransmissive mode by evaluating the synthesis of their neurotransmitter, and whether its action would be direct or through its well-known angiogenic activity...
2018: Frontiers in Molecular Neuroscience
Xinhui Wu, Zhenjie Hu, Yanling Yin, Yong Li, Tao Zhang
OBJECTIVE: To observe the effect of different doses and timing of normal saline (NS) resuscitation combined with norepinephrine (NE) on endothelial glycocalyx in rabbits with early septic shock. METHODS: Thirty New Zealand male rabbits were randomly divided into sham group, model group, 30 mL and 60 mL timely resuscitation groups (30 mL and 60 mL timely group), and 30 mL delayed resuscitation group (30 mL delayed group) with 6 rabbits in each group. The rabbit model of septic shock was reproduced by cecal ligation and puncture (CLP)...
July 2018: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
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