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Endothelial immunohistochemistry

Sonia L Hernandez, Mildred Nelson, Georgia R Sampedro, Naina Bagrodia, Ann M Defnet, Bianca Lec, Jared Emolo, Rebecca Kirschner, Lydia Wu, Henry Biermann, Stephanie Shen, Juliane Bubeck Wardenburg, Jessica J Kandel
Staphylococcus aureus infection is one of the leading causes of morbidity in hospitalized patients in the United States, an effect compounded by increasing antibiotic resistance. The secreted agent hemolysin alpha toxin (Hla) requires the receptor A Disintegrin And Metalloproteinase domain-containing protein 10 (ADAM10) to mediate its toxic effects. We hypothesized that these effects are in part regulated by Notch signaling, for which ADAM10 activation is essential. Notch proteins function in developmental and pathological angiogenesis via the modulation of key pathways in endothelial and perivascular cells...
October 15, 2018: Angiogenesis
Raimund H M Preidl, Patrick Möbius, Manuel Weber, Kerstin Amann, Friedrich W Neukam, Marco Kesting, Carol-Immanuel Geppert, Falk Wehrhan
BACKGROUND: Microvascular free flap reconstruction has become a standard technique in head and neck reconstructive surgery. Pre-operative radiotherapy is associated with a higher incidence of free flap malperfusion and the need for operative revision. Irradiated vessels present characteristic histomorphological and structural changes. Alterations in endothelial cells of irradiated arteries remain incompletely investigated especially with regard to long-term changes in endothelial dysfunction supporting an intraluminal pro-thrombotic and pro-inflammatory milieu...
October 15, 2018: Strahlentherapie und Onkologie: Organ der Deutschen Röntgengesellschaft ... [et Al]
Helen Zirnask, Pasi Pöllanen, Siim Suutre, Marianne Kuuslahti, Andres Kotsar, Tomi Pakarainen, Kersti Kokk
The aim of this study is to investigate the expression of the luteinizing hormone/choriogonadotropin (LHCG) receptor in the human penis to see, if the luteinizing hormone (LH) effects are possible in the spongious and cavernous tissue of the penis. The number of men with erection disturbances increases significantly simultaneously with the elevated LH concentrations between 40 and 70 years. It is possible that the elevated LH concentrations may influence locally the erectile mechanisms. The precondition for this is the expression of LHCG receptors in the penis...
October 15, 2018: Aging Male: the Official Journal of the International Society for the Study of the Aging Male
Gang Yu, Yupeng Guang, Jingdong Li, Jiansheng Xing, Zhiming Bai
AIMS: Testicular ischemia-reperfusion (IR) injury is the primary pathophysiological consequence of testicular torsion. Low-energy shock wave (LESW) is an effective treatment for certain diseases. The present study investigated whether LESW could improve on testicular IR injury in rats and examined the involved mechanism. MAIN METHODS: Testicular reperfusion was induced in rats after 1-h ischemia. The first LESW treatment was performed 30 min prior to testicular reperfusion, and then every other day for another 3 applications...
October 12, 2018: Life Sciences
Noriko Sugasawa, Ayako Katagi, Hirotsugu Kurobe, Taisuke Nakayama, Chika Nishio, Hiroko Takumi, Fumiharu Higashiguchi, Ken-Ichi Aihara, Michio Shimabukuro, Masataka Sata, Tetsuya Kitagawa
OBJECTIVE: Hesperidin, an abundant flavonoid in citrus fruit, and its aglycone, hesperetin, have been reported to possess various physiological activities, including antioxidant, anti-inflammatory, hypolipidemic, and antihypertensive activities. In this study, we investigated whether α-glucosyl hesperidin and water-dispersible hesperetin have protective effects on atherosclerotic progression in apolipoprotein E knockout (Apo-E KO) mice. METHODS: Ten-week-old male Apo-E KO mice were randomly assigned a regular high-fat diet, a high-fat diet with 0...
October 15, 2018: Journal of the American College of Nutrition
Marta Feito-Rodríguez, Alba Sánchez-Orta, Raúl De Lucas, Juan Carlos López-Gutiérrez, Elena Ruiz-Bravo, Eulalia Baselga, Ana M Victoria, Angela Hernández-Martín, Minia Campos-Domínguez, Beatriz Berenguer Fröhner, Gloria Garnacho-Saucedo, Angel Vera Casaño, Asunción Vicente Villa, José Bernabeu-Wittel, Heinz Kutzner, Luis Requena
BACKGROUND: Recent reports indicate that tufted angioma is a rare vascular neoplasm that manifests more frequently at birth than previously thought. Few studies specifically address congenital presentation. OBJECTIVES: We analyzed the clinicopathological characteristics, clinical course, and treatment of congenital tufted angioma (cTA) and evaluated variables that were indicative of problematic lesions. METHODS: We performed an observational retrospective study of 30 patients with cTA in 9 Spanish hospitals over a 14-year period...
October 15, 2018: Pediatric Dermatology
Lifang Zhu, Waruna Lakmal Dissanayaka, Chengfei Zhang
OBJECTIVES: The current study aimed to investigate the effects of vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1α (SDF-1α) overexpressing dental pulp stem cells (DPSCs) in vascularized dental pulp regeneration in vivo. MATERIALS AND METHODS: Human DPSCs were transfected with VEGF or SDF-1α using premade lentiviral particles. Overexpression was verified by quantitative polymerase chain reaction (q-PCR), enzyme-linked immunosorbent assay (ELISA), and western blot analysis...
October 12, 2018: Clinical Oral Investigations
Young Shin Song, Min Joo Kim, Hyun Jin Sun, Hwan Hee Kim, Hyo Shik Shin, Young A Kim, Byung-Chul Oh, Sun Wook Cho, Young Joo Park
PURPOSE: TSH suppression is widely used to treat well-differentiated thyroid cancer, while its role in poorly differentiated thyroid cancer (PDTC) is undetermined. Besides thyrocytes, TSH also binds to stromal cells, comprising tumor microenvironments. This study aimed to investigate the effects of TSH on tumor microenvironments in PDTC. EXPERIMENTAL DESIGN: An ectopic tumor model using PDTC cells (BHP10-3SCp and FRO), which exhibit TSH/cAMP-independent cell growth, were treated with TSH...
October 12, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Min-Keun Song, Eun-Jong Kim, Jung-Kook Kim, Hyeng-Kyu Park, Sam-Gyu Lee
OBJECTIVE: This study investigated the effect of regular swimming exercise according to the duration-intensity on neurocognitive function in a cerebral infarction rat model. METHODS: Forty male Sprague-Dawley 10-week-old rats, weighing 300 ± 50 g, were subjected to photothrombotic cerebral infarction. The remaining 36 rats were randomly divided into four groups (n = 9 per group: non-exercise (group A); swimming exercise of short duration-intensity (5 min/day, group B); swimming exercise of moderate duration-intensity (10 min/day, group C); and swimming exercise of long duration-intensity (20 min/day, group D)...
October 12, 2018: Neurological Research
Chun-Jie Gu, Feng Xie, Bing Zhang, Hui-Li Yang, Jiao Cheng, Yin-Yan He, Xiao-Yong Zhu, Da-Jin Li, Ming-Qing Li
BACKGROUND/AIMS: Uterus endometrial cancer (UEC) is the common malignancy among gynecologic cancers, and most of them are type I estrogen-dependent UEC. Diabetes is well-known risk factor for the development of UEC. However, the underlying link between high glucose (HG) and the estrogen receptor in UEC remains unclear. Epithelial-mesenchymal transition (EMT) has also been shown to occur during the initiation of metastasis in cancer progression. The aim of this study was to determine the relationships and roles of HG, estrogen receptor and EMT in the growth and migration of UEC...
October 11, 2018: Cellular Physiology and Biochemistry
Bee S Chew, Razmin Ghazali, Hanita Othman, Nor A M Ismail, Aimi S Othman, Nur M S T Laim, Yin P Wong, Geok C Tan
AIM: The aim of our study was to determine the endocan-1 expression in placenta of hypertensive women, and its association with maternal and fetal outcomes. METHODS: This was a cross-sectional study consisted of 21 pregnant women with hypertension and 23 without hypertension. The gestational age ranged from 28 to 39 weeks (hypertensive) and 32 to 40 weeks (normotensive). The paraffin embedded formalin fixed placenta tissue blocks were retrieved from the pathology archives...
October 10, 2018: Journal of Obstetrics and Gynaecology Research
Ramón Franco-Topete, José Sergio Zepeda-Nuño, Ana Lourdes Zamora-Perez, Martha Graciela Fuentes-Lerma, Belinda Claudia Gómez-Meda, Celia Guerrero-Velázquez
OBJECTIVE: Chronic periodontitis (CP) is characterized by gingival inflammation and bone destruction. It has been reported that interferon-gamma (IFN-γ) levels are high in CP patients; however, the IFN-γ receptor (IFN-γR) has not been studied in gingival tissue from these patients. To evaluate IFN-γ levels and IFN-γR expression in gingival tissue biopsies from chronic periodontitis patients compared with healthy subjects (HS). MATERIAL AND METHODS: Gingival tissues were obtained from all study subjects, CP (n = 18) and healthy subjects (HS) (n = 12)...
October 4, 2018: Journal of Applied Oral Science: Revista FOB
R L Figueira, F L Gonçalves, A R Prado, M C Ribeiro, K M Costa, O Castro E Silva, L Sbragia
Neonatal asphyxia occurs due to reduction in oxygen supply to vital organs in the newborn. Rapid restoration of oxygen to the lungs after a long period of asphyxia can cause lung injury and decline of respiratory function, which result from the activity of molecules that induce vascular changes in the lung such as nitric oxide (NO) and vascular endothelial growth factors (VEGF). In this study, we evaluated the pulmonary and vascular morphometry of rats submitted to the model of neonatal asphyxia and mechanical ventilation, their expression of pulmonary VEGF, VEGF receptors (VEGFR-1/VEGFR-2), and endothelial NO synthase (eNOS)...
October 8, 2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
Maojing Wang, Libo Sun, Wei Ding, Shanglang Cai, Qing Zhao
BACKGROUND: This study aimed to identify the microRNAs implicated in atrial fibrillation (AF) to investigate the molecular mechanisms underlying the role of microRNAs in ablation-based AF treatment. METHODS: Real-time polymerase chain reaction (PCR) and microRNA microarrays were utilized to measure the profiles of microRNA expression in AF to identify differentially expressed microRNAs. Enzyme-linked immunosorbent assay, real-time PCR, Western blot analysis, and immunohistochemistry assays were also performed to investigate the regulatory relationships among various factors implicated in AF...
October 9, 2018: Journal of Cellular Biochemistry
Rong Yin, Shawn J Rice, Jinwei Wang, Lin Gao, Joseph Tsai, Radean T Anvari, Fang Zhou, Xin Liu, Gang Wang, Yuxin Tang, Martin C Mihm, Chandra P Belani, Dong-Bao Chen, J Stuart Nelson, Wenbin Tan
INTRODUCTION: Port wine stain (PWS) is characterized as a progressive dilatation of immature venule-like vasculatures which result from differentiation-impaired endothelial cells. In this study, we aimed to identify the major biological pathways accounting for the pathogenesis of PWS. METHODS: Sequential windowed acquisition of all theoretical fragment ion mass spectra (SWATH-MS) was used to identify differentially expressed proteins in PWS lesions, followed by confirmative studies with immunohistochemistry, immunoblot and transmission electron microscopy (TEM)...
October 10, 2018: Histology and Histopathology
Hyun Kyung Park, Su Kang Kim, Sang Won Lee, Joo-Ho Chung, Byung-Cheol Lee, Sae Won Na, Chun Gun Park, Young Ock Kim
A recent study reported that Panax ginseng ( P . ginseng ) has a protective effect on the development of benign prostatic hyperplasia (BPH). KH053 is used as a new herbal prescription consisting of P . ginseng and bee-pollen. The present study aimed to investigate whether the KH053 has inhibition effects on the development of benign prostatic hyperplasia (BPH) using an animal model with testosterone induced BPH. The experiment was carried out in forty male Wistar 7 week old rats that were divided into four groups (control group, BPH group, positive group, and KH053 group)...
November 2017: Saudi Journal of Biological Sciences
Jessica L Turnier, Hermine I Brunner, Michael Bennett, Ashwaq Aleed, Gaurav Gulati, Wendy D Haffey, Sherry Thornton, Michael Wagner, Prasad Devarajan, David Witte, Kenneth D Greis, Bruce Aronow
Objectives: We used an unbiased proteomics approach to identify candidate urine biomarkers (CUBMs) predictive of LN chronicity and pursued their validation in a larger cohort. Methods: In this cross-sectional pilot study, we selected urine collected at kidney biopsy from 20 children with varying levels of LN damage (discovery cohort) and performed proteomic analysis using isobaric tags for relative and absolute quantification (iTRAQ). We identified differentially excreted proteins based on degree of LN chronicity and sought to distinguish markers exhibiting different relative expression patterns using hierarchically clustered log10-normalized relative abundance data with linked and distinct functions by biological network analyses...
October 4, 2018: Rheumatology
Wen-Zhu Li, Yi Yang, Kun Liu, Rui Long, Nan Jin, Shi-Yuan Huang, Ya You, Jing Dai, Cheng Fan, Jue Wang, Zhao-Hui Wang
BACKGROUND: Membrane-associated fibrinogen-like protein 2 (FGL2 prothrombinase, pFGL2) is abundantly expressed in activated microvascular endothelial cells (MVECs) and plays a crucial role in microthrombus formation in microcirculatory vasculature. It has been widely reported that coronary microvascular obstruction (CMVO) contributes to adverse outcomes following myocardial ischemia/reperfusion. However, the role of pFGL2 in CMVO is poorly understood. METHODS AND RESULTS: We aimed to identify the effect of MVECs-pFGL2 in CMVO using FGL2 knockout mice...
September 13, 2018: International Journal of Cardiology
Jun Jiang, Shun Wang, Yuan Chen, Chengwei Wang, Chuncheng Qu, Yuguang Liu
Glial tumors constitute the majority of primary intracranial brain tumors. The expression of specific markers of lymphangiogenesis in gliomas still remains unclear.A total of 40 surgical specimens from 20 patients with recurrent gliomas were included in the study. The expression of D2-40, vascular endothelial growth factor (VEGF)-C, VEGF-D, and VEGF receptor-3 (VEGR-3) was detected by immunohistochemistry (IHC). The clinicopathologic data (p53 and Ki67) were also collected and analyzed.At relapse malignant transformation rate was 65% (13/20 cases)...
September 2018: Medicine (Baltimore)
Hotaka Kawai, Hidetsugu Tsujigiwa, Chong Huat Siar, Keisuke Nakano, Kiyofumi Takabatake, Masae Fujii, Mei Hamada, Ryo Tamamura, Hitoshi Nagatsuka
Background: The tumor microenvironment and its stromal cells play an important role in cancer development and metastasis. Bone marrow-derived cells (BMDCs), a rich source of hematopoietic and mesenchymal stem cells, putatively contribute to this tumoral stroma. However their characteristics and roles within the tumor microenvironment are unclear. In the present study, BMDCs in the tumor microenvironment were traced using the green fluorescent protein (GFP) bone marrow transplantation model. Methods: C57BL/6 mice were irradiated and rescued by bone marrow transplantation from GFP-transgenic mice...
2018: International Journal of Medical Sciences
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