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WT-1 vascular

Min Liang, Zhongmin Chen, Fuping Wang, Lan Liu, Runan Wei, Mei Zhang
Few burn dressings can self-regulate the optimal humidity levels that are required for wound healing, while also providing good anti-adhesive properties to prevent damage that can occur when wound dressings are changed. Consequently, a water-soluble carboxymethylcellulose sodium/sodium alginate/chitosan (CMC-Na/SA/CS) composite hydrogel has been developed as a potential burn wound dressing, with orthogonal testing revealing an optimal ratio of CMC-Na, SA, and CS as 2, 3, and 1 wt % for hydrogel preparation, respectively...
October 8, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Dong-Jie Li, Jie Tong, Fei-Yan Zeng, Mengqi Guo, Yong-Hua Li, Hongbo Wang, Pei Wang
BACKGROUND AND PURPOSE: Platelet-derived growth factor-BB (PDGF-BB) is an angiogenic factor involved in cardiovascular diseases. Here, we investigated the possible effects of activation of nicotinic acetylcholine receptor α7 subtype (α7nAChR) on PDGF-BB-induced proliferation and migration in vascular smooth muscle cells (VSMCs). EXPERIMENTAL APPROACH: PNU-282987, a selective α7nAChR pharmacological agonist, was used to activate α7nAChR. The influences of α7nAChR activation on PDGF-BB-induced proliferation and migration, as well as the phosphorylation of focal adhesion kinase/Src, a pro-migration signaling pathway, were determined in VSMCs...
September 30, 2018: British Journal of Pharmacology
Shurui Liao, Qiang He, Lie Yang, Shuai Liu, Ze Zhang, Robert Guidoin, Qiang Fu, Xingyi Xie
We screened a family of nonspecific cell-repelling polyurethanes (PUs) whose backbones are attached with epoxy group-terminated polyethylene glycol (PEG) side chains. Water incubation of the PU films (with 9.2-31.1 wt % PEG) caused a surface enrichment of PEG chains where vascular endothelial growth factor (VEGF) was grafted by forming secondary amine linkages between VEGF molecules and the PEG spacer. These linkages are still ionizable similar to original primary amines in VEGF, thereby retaining the original charge distribution on VEGF macromolecules...
September 28, 2018: Journal of Biomedical Materials Research. Part B, Applied Biomaterials
Rehan Khan, Muneeb U Rehman, Abdul Quaiyoom Khan, Mir Tahir, Sarwat Sultana
OBJECTIVES: Colon cancer is the major health disease related with high mortality. Glycyrrhizic acid (GA) is an active constituent of licorice with anti-inflammatory and anticarcinogenesis effects. We investigated the chemopreventive potential of GA against 1,2-dimethylhydrazine (DMH)-induced colon tumorigenesis in Wistar rats. METHODS: Glycyrrhizic acid was administered orally at the dose of 15 mg/kg b.wt. and DMH was administered at the dose of 20 mg/kg b.wt...
September 26, 2018: Environmental Toxicology
Abdelrahman Y Fouda, Zhimin Xu, Esraa Shosha, Tahira Lemtalsi, Jijun Chen, Haroldo A Toque, Rebekah Tritz, Xuezhi Cui, Brian K Stansfield, Yuqing Huo, Paulo C Rodriguez, Sylvia B Smith, R William Caldwell, S Priya Narayanan, Ruth B Caldwell
The lack of effective therapies to limit neurovascular injury in ischemic retinopathy is a major clinical problem. This study aimed to examine the role of ureohydrolase enzyme, arginase 1 (A1), in retinal ischemia-reperfusion (IR) injury. A1 competes with nitric oxide synthase (NOS) for their common substrate L-arginine. A1-mediated L-arginine depletion reduces nitric oxide (NO) formation by NOS leading to vascular dysfunction when endothelial NOS is involved but prevents inflammatory injury when inducible NOS is involved...
September 25, 2018: Cell Death & Disease
Yunhong Li, Jianxin Han, Yufeng Chen, Chun Chen, Bingquan Chu, Ying Zhang
AIMS: Previous studies indicate that the anti-hypoxia effects of Tibetan Turnip (Brassica rapa ssp. rapa) were closely related to its characteristic components being p-coumaric acid (CA) and p-coumaric acid‑β‑d‑glucopyranoside (CAG). Since CAG would be converted to CA in vivo, this study aims to further examine the efficacy and mechanism of CA against pulmonary edema induced by normobaric hypoxia. MAIN METHODS: Male ICR mice were assigned to the normoxia group and several hypoxia groups, given sterile water, CA or dexamethasone orally, once daily for four consecutive days...
October 15, 2018: Life Sciences
Verena Peters, Vittorio Calabrese, Elisabete Forsberg, Nadine Volk, Thomas Fleming, Hans Baelde, Tim Weigand, Christian Thiel, Angela Trovato, Maria Scuto, Sergio Modafferi, Claus Peter Schmitt
BACKGROUND/AIMS: In rodents, carnosine treatment improves diabetic nephropathy, whereas little is known about the role and function of anserine, the methylated form of carnosine. METHODS: Antioxidant activity was measured by oxygen radical absorbance capacity and oxygen stress response in human renal tubular cells (HK-2) by RT-PCR and Western-Immunoblotting. In wildtype (WT) and diabetic mice (db/db), the effect of short-term anserine treatment on blood glucose, proteinuria and vascular permeability was measured...
September 13, 2018: International Journal of Molecular Sciences
Mahboubeh Jafarkhani, Zeinab Salehi, Mohammad Ali Shokrgozar, Shohreh Mashayekhan
The development of microfluidic hydrogels is an attractive method to generate continues perfusion, induce vascularization, increase solutes delivery and ultimately improve cell viability. However, the transport processes in many in vitro studies still have not been realized completely. To address this problem, we have developed a micro-channeled hydrogel with different collagen type I concentration of 1, 2 and 3 wt.% and assessed its physical properties and obtained diffusion coefficient of nutrient within the hydrogel...
September 14, 2018: International Journal for Numerical Methods in Biomedical Engineering
Hui Fu, Ji-Kuai Chen, Wen-Jie Lu, Yu-Jie Jiang, Yuan-Yuan Wang, Dong-Jie Li, Fu-Ming Shen
Backgrounds and Aims: Na+ is an important nutrient and its intake, mainly from salt (NaCl), is essential for normal physiological function. However, high salt intake may lead to vascular injury, independent of a rise in blood pressure (BP). Canonical NALP3 inflammasome activation is a caspase-1 medicated process, resulting in the secretion of IL-18 and IL-1β which lead to endothelial dysfunction. However, some researches uncovered a direct and inflammasome-independent role of NALP3 in renal injury. Thus, this study was designed to investigate the possible mechanisms of NALP3 in high salt induced endothelial dysfunction...
2018: Frontiers in Pharmacology
Henrique Silva, Hugo A Ferreira, Hugo P da Silva, L Monteiro Rodrigues
Perfusion at microvascular level involves the contribution of both local and central regulators, under a complex vascular signaling frame. The venoarteriolar reflex (VAR) is one of such regulatory responses, of particular relevance in the lower limb to prevent edema. Although known for quite some time, many of the complex interactions involving all of these regulatory mechanisms still need clarification. Our objective was to look deeper into VAR through modern photoplethymography (PPG). Twelve healthy subjects (both sexes, 26...
2018: Frontiers in Physiology
Pratik Satya, Avrajit Chakraborty, Debabrata Sarkar, Maya Karan, Debajeet Das, Nur Alam Mandal, Dipnarayan Saha, Subhojit Datta, Soham Ray, Chandan Sourav Kar, Pran Gobinda Karmakar, Jiban Mitra, Nagendra Kumar Singh
Enzyme β-galactosidase (EC is known to influence vascular differentiation during early vegetative growth of plants, but its role in hypocotyl development is not yet fully understood. We generated the hypocotyl transcriptome data of a hypocotyl-defect jute (Corchorus capsularis L.) mutant (52,393 unigenes) and its wild-type (WT) cv. JRC-212 (44,720 unigenes) by paired-end RNA-seq and identified 11 isoforms of β-galactosidase, using a combination of sequence annotation, domain identification and structural-homology modeling...
August 30, 2018: Phytochemistry
Mark Chandy, Masayoshi Ishida, Eric A Shikatani, Omar El-Mounayri, Lawrence Changsu Park, Talat Afroze, Tao Wang, Philip A Marsden, Mansoor Husain
BACKGROUND: MicroRNAs (miR) are small non-coding RNAs that regulate diverse biological functions. The bicistronic gene miR-143/145 determines cell fate and phenotype of vascular smooth muscle cells (VSMC), in part, by destabilizing Elk-1 mRNA. The transcription factor c-Myb also regulates differentiation and proliferation of VSMC, and here we test whether these effects may be mediated by miR-143/145. METHODS & RESULTS: Flow cytometry of cardiovascular-directed d3...
2018: PloS One
J Westhoff, K Weismüller, C Koch, V Mann, M A Weigand, M Henrich
BACKGROUND: Ischemia of intestinal organs is a main cause of complications in surgical intensive care patients. Changes in the tonus of arteries contributing to vascular resistance play an important role in the determination of blood flow and thus oxygen supply of various abdominal organs. It is generally acknowledged that hypoxia itself is able to alter arterial tonus and thus blood flow. METHODS: The present study compared the effects of various degrees of hypoxia on second-order mesenteric arteries from male C57BL/6J mice...
August 25, 2018: European Journal of Medical Research
Alecsander F Bressan, Gisele A Fonseca, Rita C Tostes, R Clinton Webb, Victor Vitorino Lima, Fernanda Regina Giachini
The activation of extracellular signal-regulated kinase 1 and 2 (ERK 1/2) pathway promotes increased vascular contractility in angiotensin II (Ang II)-induced hypertensive mice. Interleukin-10 (IL-10) is an immune-regulatory cytokine with the ability to prevent vascular hypercontractility during hypertension. We hypothesized that IL-10 would downregulate vascular ERK 1/2 activation during Ang II-induced hypertension. Wild-type (WT) or IL-10 knockout (IL-10-/- ) mice received Ang II infusion (90 ηg.min) or vehicle (saline), via osmotic mini-pumps (0...
August 24, 2018: Fundamental & Clinical Pharmacology
Gema Mondejar-Parreño, Daniel Morales-Cano, Bianca Barreira, Maria Callejo, Jesus Ruiz-Cabello, Laura Moreno, Sergio Esquivel-Ruiz, Alistair Mathie, Ghazwan Butrous, Francisco Perez-Vizcaino, Angel Cogolludo
Human immunodeficiency virus (HIV) infection is an established risk factor for pulmonary arterial hypertension (PAH), however the pathogenesis of HIV-related PAH remains unclear. Since K+ channel dysfunction is a common marker in most forms of PAH, our aim was to analyse if the expression of HIV proteins is associated with impairment of K+ channel function in the pulmonary vascular bed. HIV transgenic mice (Tg26) expressing seven of the nine HIV viral proteins and wild type (Wt) mice were used. Hemodynamic assessment was performed by echocardiography and catheterization...
August 23, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
Mo Chen, Yuantao Wang, Hui Wang, Liguang Sun, Yaowen Fu, Yong-Guang Yang
CD47 is a ubiquitously expressed transmembrane glycoprotein that plays a complex role in regulation of cell survival and function. We have previously shown that the interspecies incompatibility of CD47 plays an important role in triggering rejection of cellular xenografts by macrophages. However, the role of CD47 in solid organ transplantation remains undetermined. Here, we explored this question in mouse models of heart allotransplantation. We observed that the lack of CD47 in donor hearts had no deleterious effect on graft survival in syngeneic or single MHC class I-mismatched recipients, in which both wild-type (WT) and CD47 knockout (CD47 KO) mouse hearts survived long term with no sign of rejection...
August 23, 2018: Xenotransplantation
Sally L Elshaer, Azza B El-Remessy
Ischemic retinopathy is characterized by ischemia followed by retinal neovascularization (RNV) resulting in visual impairment. Given the role of neuron-secreted growth factors in regulating angiogenesis, we examined how genetic deletion of the neurotrophin receptor; p75NTR can overcome retinal ischemia using oxygen-induced retinopathy (OIR) mouse model. Wildtype (WT) or p75NTR-/- mice pups were subjected to hyperoxia (70% O2 , p7-p12) then returned to normal air (relative hypoxia, p12-p17). Vascular alterations were assessed at p12 and p17 time-points...
August 21, 2018: Scientific Reports
Zahra Zahid Piracha, Hyeon Joong Kwon, Umar Saeed, Jumi Kim, Jaesung Jung, Yong-Joon Chwae, Sun Park, Ho-Joon Shin, Kyongmin Kim
Sirtuin 2 (Sirt2), a NAD+ -dependent protein deacetylase, is overexpressed in many hepatocellular carcinomas (HCCs) and can deacetylate many proteins, including tubulins and AKT prior to AKT activation. Here, we found that endogenous Sirt2 was upregulated in hepatitis B virus (HBV) WT-replicating cells, leading to tubulin deacetylation; however, this was not the case in HBV replication-deficient mutant-transfected cells and 1.3mer HBV WT-transfected plus reverse transcriptase inhibitor (entecavir or lamivudine) treated cells but all HBV proteins are expressed...
August 15, 2018: Journal of Virology
Daniel W Nuno, Lawrence J Coppey, Mark A Yorek, Kathryn G Lamping
We tested whether dietary fatty acids alter membrane composition shifting localization of signaling pathways within caveolae to determine their role in vascular function. Wild type (WT) and caveolin-1-deficient mice (cav-1 KO), required for vascular caveolae formation, were fed low fat (LF), high saturated fat (HF, 60% kcal from lard), or high-fat diet with 50:50 lard and n-3 polyunsaturated fatty acid-enriched menhaden oil (MO). HF and MO increased body weight and fat in WT but had less effect in cav-1 KO...
August 2018: Physiological Reports
Zhiqi Tang, Lijuan Liu, Yujie Guo, Guoxiong Deng, Meixiang Chen, Jinru Wei
The present study examined whether exendin‑4 (Ex4) can improve the endothelial dysfunction of apolipoprotein E knockout (APOE‑KO) mice fed a high‑cholesterol diet and the potential mechanism by which it acts. Genetically wild‑type (WT) C57BL/6 mice and APOE‑KO mice of C57BL/6 background, were each randomly assigned to receive either Ex4 treatment (Ex4‑treated, for 8 weeks) or not (control). The 4 groups were fed the same high‑cholesterol diet for 8 weeks. The following were measured at the end of the eighth week: Endothelium‑dependent vasodilation of the arteries; plasma nitric oxide (NO) and metabolic index; levels of endothelial NO synthase (eNOS); phosphorylated eNOS (p‑eNOS; Ser‑1,177); guanosine triphosphate cyclohydrolase‑1 (GCH1); and tetrahydrobiopterin (THB)...
September 2018: Molecular Medicine Reports
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