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https://www.readbyqxmd.com/read/30107383/pulsatility-index-as-a-novel-parameter-for-perfusion-in-mouse-model-of-hindlimb-ischemia
#1
Hairong Xu, Shan Lu, Lexi Ding, Liangkun Lyu, Zejun Ma, Qiulun Lu
BACKGROUND/AIMS: In clinical settings, the pulsatility index (PI) has become a widely used tool for monitoring obstetrics or other vascular diseases. It is based on the maximum Doppler shift waveform derived from ultrasonography. However, it remains unclear whether the PI levels are correctly predicted from the perfusion in mouse model of hindlimb ischemia. METHODS: To explore the relationship between PI and perfusion, we generated a unilateral hindlimb ischemia model in 8-week-old C57BL/6 male mice by ligation of the right common iliac artery and femoral artery...
August 14, 2018: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/30106666/expression-of-autophagy-signaling-molecules-in-the-outer-membranes-of-chronic-subdural-hematomas
#2
Koji Osuka, Yasuo Watanabe, Nobuteru Usuda, Masahiro Aoyama, Mikinobu Takeuchi, Masakazu Takayasu
Chronic subdural hematoma (CSDH) is fundamentally treatable, although it sometimes recurs. We observed, however, several cases of spontaneous resolution of CSDH outer membranes, even in a trabecular type of CSDH, after a trepanation surgical procedure. In this study, we examined the expression of molecules of the autophagy signaling pathway in CSDH outer membranes. Eight patients whose outer membranes were obtained successfully during trepanation were included in this study. By Western blot analysis, we examined the expression of mammalian target of rapamycin (mTOR); GβL; UNC-51-like kinase-1 (ULK1); Beclin-1; autophagy-related genes (Atg) 3, 5, 7, 12, 13, and 16L1β,α; the autophagy marker Light Chain3A/B (LC3A/B); and β-actin, which constitute the autophagy signaling pathway...
August 14, 2018: Journal of Neurotrauma
https://www.readbyqxmd.com/read/30106126/mir-291b-3p-mediated-ros-induced-endothelial-cell-dysfunction-by-targeting-hur
#3
Xiaofang Sui, Shuqian Yu, Lin Dou, Xiehui Chen, Xuejie Li, Jun Yang, Yanan Su, Shuyue Wang, Fengling Wang, Jian Li
Endothelial dysfunction is an early marker of atherosclerosis. Previous studies have indicated that microRNA (miR)‑291b‑3p regulates the metabolism of lipids and glucose in the liver via targeting adenosine monophosphate‑activated kinase α1 and transcription factor p65. The present study investigated whether miR‑291b‑3p mediated H2O2‑mediated endothelial dysfunction. The level of apoptosis of EOMA mouse endothelial cells was analyzed by terminal deoxynucleotidyl‑transferase‑mediated dUTP nick end labelling staining...
August 10, 2018: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/30104414/tnf-%C3%AE-elicits-phenotypic-and-functional-alterations-of-vascular-smooth-muscle-cells-by-mir-155-5p-dependent-downregulation-of-cgmp-dependent-kinase-1
#4
Seunghwan Choi, Minsik Park, Joohwan Kim, Wonjin Park, Suji Kim, Dong-Keon Lee, Jong Yun Hwang, Jongseon Choe, Moo-Ho Won, Sungwoo Ryoo, Kwon-Soo Ha, Young-Guen Kwon, Young-Myeong Kim
cGMP-dependent protein kinase 1 (PKG1) plays an important role in nitric oxide (NO)/cGMP-mediated maintenance of vascular smooth muscle cell (VSMC) phenotype and vasorelaxation. Inflammatory cytokines, including tumor necrosis factor-α (TNF-α), have long been understood to mediate several inflammatory vascular diseases. However, the underlying mechanism of TNF-α-dependent inflammatory vascular disease is unclear. Here, we found that TNF-α treatment decreased PKG1 expression in cultured VSMCs, which correlated with NF-κB-dependent biogenesis of miR-155-5p that targeted the 3'-untranslated region of PKG1 mRNA...
August 13, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/30104096/reversible-secretome-and-signaling-defects-in-diabetic-mesenchymal-stem-cells-from-peripheral-arterial-disease-patients
#5
Tatiana Chadid, Andrew Morris, Alexandra Surowiec, Scott Robinson, Maiko Sasaki, Jacques Galipeau, Brian P Pollack, Luke P Brewster
OBJECTIVE: Regenerative medicine seeks to stall or to reverse the pathologic consequences of chronic diseases. Many people with diabetes have peripheral arterial disease (PAD), which increases their already high risk of major amputation. Cellular therapies are a promising regenerative medicine approach to PAD that can be used to focally inject regenerative cells to endangered tissue beds. Mesenchymal stem cells (MSCs) are known to promote tissue regeneration through stromal support and paracrine stimulation of new blood vessels (angiogenesis)...
August 10, 2018: Journal of Vascular Surgery
https://www.readbyqxmd.com/read/30103817/injury-induced-fetal-reprogramming-imparts-multipotency-and-reparative-properties-to-pericardial-adipose-stem-cells
#6
Jianfeng Tang, Xiaoming Wang, Kezhe Tan, Hongtao Zhu, Youming Zhang, Weili Ouyang, Xueqing Liu, Zhaoping Ding
BACKGROUND: Injury may induce a sequential activation of intrinsic reparative activity that supports the maintenance of tissue homeostasis. METHOD: In the present experiments, we investigated whether myocardial infarction (MI) was able to reinstate the expression of Wilms' tumor factor 1 (WT1) as a key hallmark of fetal reprograming in the pericardial adipose-derived stem cells (pADSC). We characterized the immunophenotypical markers, cardiac potential, and reparative activity of WT1-expressing pADSC (WT1pos ) isolated MI Wistar rats with an intact pericardial sac in which cardiac transudate was accumulated, sampled, and analyzed...
August 13, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/30102768/neoplastic-transformation-of-peribiliary-stem-cell-niche-in-cholangiocarcinoma-arisen-in-primary-sclerosing-cholangitis
#7
Guido Carpino, Vincenzo Cardinale, Trine Folseraas, Diletta Overi, Krzysztof Grzyb, Daniele Costantini, Pasquale Bartolomeo Berloco, Sabina Di Matteo, Tom Hemming Karlsen, Domenico Alvaro, Eugenio Gaudio
Primary sclerosing cholangitis (PSC) is a chronic inflammatory cholangiopathy frequently complicated by cholangiocarcinoma (CCA). Massive proliferation of biliary tree stem/progenitor cells (BTSCs), expansion of peribiliary glands (PBGs), and dysplasia were observed in PSC. The aims of the present study were to evaluate the involvement of PBGs and BTSCs in CCA which emerged in PSC patients. Specimens from normal liver (N=5), PSC (N=20), and PSC-associated CCA (N=20) were included. Samples were processed for histology, immunohistochemistry and immunofluorescence...
August 13, 2018: Hepatology: Official Journal of the American Association for the Study of Liver Diseases
https://www.readbyqxmd.com/read/30101695/the-possible-impact-of-hyperuricemia-on-serum-soluble-receptor-for-advanced-glycation-end-products-srage-levels-in-teenagers-a-case-control-study
#8
M Lemiesz, E Tenderenda-Banasiuk, D Sosnowska, A Rybi-Szuminska, J Storonowicz, T Lemiesz, A Wasilewska
Background Dietary advanced glycation end products (AGEs) and their interactions with the soluble receptors for AGEs (RAGE) play a crucial role in the pathogenesis of cardiovascular diseases. Objective This study was set out to assess, whether there was any association between serum sRAGE level and serum uric acid level in children with hyperuricemia Methods This case control study involved 53 patients (12 girls, 41 boys) with hyperuricemia (defined as serum uric acid >4.8 and >5.5 mg/dl in girls and boys, respectively) aged median of 13...
August 12, 2018: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/30101670/3d-spheroid-primary-human-hepatocytes-in-mono-and-co-culture-with-non-parenchymal-cells
#9
Audrey Baze, Céline Parmentier, Delilah F G Hendriks, Tracey Hurrell, Bruno Heyd, Phillipe Bachellier, Catherine Schuster, Magnus Ingelman-Sundberg, Lysiane Richert
Recent advances in the development of various culture platforms are promising for achieving more physiologically relevant in vitro hepatic models using primary human hepatocytes (PHH). Previous studies have shown the value of PHH 3D-spheroid models, cultured in low cell number (1330-2000 cells/3D-spheroid), to study long-term liver function as well as pharmacological drug effects and toxicity. Here we report that only plateable PHH aggregate and form compact 3D-spheroids with a success rate of 79%, with 96% reproducibility...
August 13, 2018: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/30101349/kinetics-of-soluble-mediators-of-the-host-response-in-ebola-virus-disease
#10
Romy Kerber, Ralf Krumkamp, Misa Korva, Toni Rieger, Stephanie Wurr, Sophie Duraffour, Lisa Oestereich, Martin Gabriel, Daouda Sissoko, Xavier Anglaret, Denis Malvy, Jürgen May, Tatjana Avšic Županc, César Muñoz-Fontela, Stephan Günther
Background: The pathophysiology of Ebola virus disease (EVD) is still poorly understood. This study aimed at identifying soluble biomarkers that inform on disease mechanisms. Methods: Fifty-four soluble mediators of the immune, coagulation, and endothelial system were measured in baseline and follow-up samples from hospitalized patients with EVD, using Luminex technology. Cross-sectional expression levels and changes over time were correlated with outcome. Results: Levels of circulating proinflammatory cytokines and chemokines, as well as markers of endothelial dysfunction and coagulopathy, were elevated on admission to hospital in patients who died from EVD as compared to survivors...
August 8, 2018: Journal of Infectious Diseases
https://www.readbyqxmd.com/read/30101151/a-library-based-screening-strategy-for-the-identification-of-darpins-as-ligands-for-receptor-targeted-aav-and-lentiviral-vectors
#11
Jessica Hartmann, Robert C Münch, Ruth-Therese Freiling, Irene C Schneider, Birgit Dreier, Washington Samukange, Joachim Koch, Markus A Seeger, Andreas Plückthun, Christian J Buchholz
Delivering genes selectively to the therapeutically relevant cell type is among the prime goals of vector development. Here, we present a high-throughput selection and screening process that identifies designed ankyrin repeat proteins (DARPins) optimally suited for receptor-targeted gene delivery using adeno-associated viral (AAV) and lentiviral (LV) vectors. In particular, the process includes expression, purification, and in situ biotinylation of the extracellular domains of target receptors as Fc fusion proteins in mammalian cells and the selection of high-affinity binders by ribosome display from DARPin libraries each covering more than 1012 variants...
September 21, 2018: Molecular Therapy. Methods & Clinical Development
https://www.readbyqxmd.com/read/30100749/cell-adhesion-molecules-and-exercise
#12
REVIEW
Yunsuk Koh, Jinkyung Park
Background: An extensive systematic review was undertaken in the current literature in order to explore the role of different types and intensities of exercise in cell adhesion molecules (CAMs), one of the markers of vascular inflammation, endothelial dysfunction, and atherosclerosis. Methods: Twenty-eight qualifying studies were extensively reviewed to examine the effects of different intensities (low-to-moderate vs. high) and types (aerobic vs. resistance) of exercise on intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and selectins...
2018: Journal of Inflammation Research
https://www.readbyqxmd.com/read/30100403/epigenetic-prediction-of-response-to-anti-pd-1-treatment-in-non-small-cell-lung-cancer-a-multicentre-retrospective-analysis
#13
Michäel Duruisseaux, Anna Martínez-Cardús, Maria E Calleja-Cervantes, Sebastian Moran, Manuel Castro de Moura, Veronica Davalos, David Piñeyro, Montse Sanchez-Cespedes, Nicolas Girard, Marie Brevet, Etienne Giroux-Leprieur, Coraline Dumenil, Monica Pradotto, Paolo Bironzo, Enrica Capelletto, Silvia Novello, Alexis Cortot, Marie-Christine Copin, Niki Karachaliou, Maria Gonzalez-Cao, Sergio Peralta, Luis M Montuenga, Ignacio Gil-Bazo, Iosune Baraibar, Maria D Lozano, Mar Varela, Jose C Ruffinelli, Ramon Palmero, Ernest Nadal, Teresa Moran, Lidia Perez, Immaculada Ramos, Qingyang Xiao, Agustin F Fernandez, Mario F Fraga, Marta Gut, Ivo Gut, Cristina Teixidó, Noelia Vilariño, Aleix Prat, Noemi Reguart, Amparo Benito, Pilar Garrido, Isabel Barragan, Jean-François Emile, Rafael Rosell, Elisabeth Brambilla, Manel Esteller
BACKGROUND: Anti-programmed death-1 (PD-1) treatment for advanced non-small-cell lung cancer (NSCLC) has improved the survival of patients. However, a substantial percentage of patients do not respond to this treatment. We examined the use of DNA methylation profiles to determine the efficacy of anti-PD-1 treatment in patients recruited with current stage IV NSCLC. METHODS: In this multicentre study, we recruited adult patients from 15 hospitals in France, Spain, and Italy who had histologically proven stage IV NSCLC and had been exposed to PD-1 blockade during the course of the disease...
August 9, 2018: Lancet Respiratory Medicine
https://www.readbyqxmd.com/read/30100173/connexin-30-2-is-expressed-in-exocrine-vascular-endothelial-and-ductal-epithelial-cells-throughout-pancreatic-postnatal-development
#14
C Coronel-Cruz, I Sánchez, B Hernández-Tellez, V Rodríguez-Mata, E Pinzón-Estrada, A Castell-Rodríguez, E M Pérez-Armendariz
Previously we have demonstrated that the GJ protein connexin 30.2 (Cx30.2) is expressed in pancreatic beta cells and endothelial cells (ECs) of the islet. In the present study, we address whether Cx30.2 is expressed in the exocrine pancreas, including its vascular system. For this, adult mouse pancreatic sections were double labeled with specific antibodies against Cx30.2 and CD31, an endothelial cell marker, or with anti-α-actin smooth muscle, a smooth muscle cell (SMC) marker or anti-mucin-1, a marker of epithelial ductal cells, using immunofluorescence (IF) studies...
August 2018: Acta Histochemica
https://www.readbyqxmd.com/read/30099394/evaluation-of-capillary-pathologies-by-nailfold-capillaroscopy-in-patients-with-psoriasis-vulgaris-study-protocol-for-a-prospective-controlled-exploratory-study
#15
Christine Fink, Samuel Kilian, Ines Bertlich, Elti Hoxha, Felicitas Bardehle, Alexander Enk, Holger A Haenssle
INTRODUCTION: Psoriasis vulgaris was shown to be an independent factor increasing the risk of several comorbidities such as obesity, diabetes and dyslipidaemia with an increased risk of stroke and myocardial infarction. We hypothesise that early endothelial dysfunction, which plays a crucial role in the pathogenesis of atherosclerosis, may be detected by digital video nailfold capillaroscopy (DVNC) at the level of the dermal capillary microvasculature as a surrogate parameter. Nailfolds represent the only body site allowing for a non-invasive assessment of the capillary microvasculature at a horizontal plane...
August 10, 2018: BMJ Open
https://www.readbyqxmd.com/read/30099336/generation-of-nine-induced-pluripotent-stem-cell-lines-as-an-ethnic-diversity-panel
#16
Xiugong Gao, Jeffrey J Yourick, Robert L Sprando
Human induced pluripotent stem cells (iPSCs) provide a potentially unlimited source of differentiated cells from individuals with specific genetic backgrounds. Using self-replicative RNA reprogramming technology, we generated nine iPSC lines from endothelial progenitor cells (EPCs) derived from blood samples of three different ethnicities: Black or African American, Latino or Hispanic, and Non-Hispanic White. The resulting iPSC lines showed normal karyotype in large part, expressed pluripotency marker genes, and spontaneously differentiated in vitro into the three germ layers...
July 27, 2018: Stem Cell Research
https://www.readbyqxmd.com/read/30099278/coupling-of-a-specific-photoreactive-triple-helical-peptide-to-crosslinked-collagen-films-restores-binding-and-activation-of-ddr2-and-vwf
#17
Jean-Daniel Malcor, Victoria Juskaite, Despoina Gavriilidou, Emma J Hunter, Natalia Davidenko, Samir Hamaia, Sanjay Sinha, Ruth E Cameron, Serena M Best, Birgit Leitinger, Richard W Farndale
Collagen-based scaffolds may require chemical crosslinking to achieve mechanical properties suitable for tissue engineering. Carbodiimide treatment, often used for this purpose, consumes amino acid side chains required for receptor recognition, thus reducing cell-collagen interaction. Here, we restore recognition and function of both von Willebrand Factor (VWF) and Discoidin Domain Receptor 2 (DDR2) to crosslinked collagen films by derivatisation with a specific triple-helical peptide (THP), an approach previously applied to integrin-mediated cellular adhesion...
July 31, 2018: Biomaterials
https://www.readbyqxmd.com/read/30098392/rate-of-small-molecular-drug-transport-across-the-blood-brain-barrier-in-a-pericyte-deficient-state
#18
Nebojsa Mihajlica, Christer Betsholtz, Margareta Hammarlund-Udenaes
Close interactions between pericytes and brain endothelial cells are essential for keeping the blood-brain barrier (BBB) functional and to restrict the transport of various xenobiotics from blood circulation to the brain parenchyma. Profound understanding of pericyte roles at the BBB and underlying mechanisms for the regulation of BBB transport are important as a potential strategy to improve drug delivery in treatment of CNS disorders. The aim of the present study was to investigate pericyte role in the rate of small-molecular drug transport across the BBB, by examining three model compounds in a pericyte-deficient state...
August 8, 2018: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/30097810/cmtm4-regulates-angiogenesis-by-promoting-cell-surface-recycling-of-ve-cadherin-to-endothelial-adherens-junctions
#19
Ihsan Chrifi, Laura Louzao-Martinez, Maarten M Brandt, Christian G M van Dijk, Petra E Bürgisser, Changbin Zhu, Johan M Kros, Marianne C Verhaar, Dirk J Duncker, Caroline Cheng
Vascular endothelial (VE) cadherin is a key component of endothelial adherens junctions (AJs) and plays an important role in maintaining vascular integrity. Endocytosis of VE-cadherin regulates junctional strength and a decrease of surface VE-cadherin reduces vascular stability. However, disruption of AJs is also a requirement for vascular sprouting. Identifying novel regulators of endothelial endocytosis could enhance our understanding of angiogenesis. Here, we evaluated the angiogenic potential of (CKLF-like MARVEL transmembrane domain 4) CMTM4 and assessed in which molecular pathway CMTM4 is involved during angiogenesis...
August 10, 2018: Angiogenesis
https://www.readbyqxmd.com/read/30097337/circulating-long-noncoding-rna-dkfzp434i0714-predicts-adverse-cardiovascular-outcomes-in-patients-with-end-stage-renal-disease
#20
Chun-Fu Lai, Yen-Ting Chen, Jian Gu, Jeanne M Nerbonne, Chin-Hsien Lin, Kai-Chien Yang
BACKGROUND: Cardiovascular (CV) diseases are major causes of mortality in uremic patients. Conventional risk factors fail to identify uremic patients with increased propensity for adverse CV outcomes. We aimed to test the hypothesis that circulating long noncoding RNAs (lncRNAs) could be a prognostic marker to predict adverse CV outcomes in uremic patients. METHODS AND RESULTS: Plasma lncRNAs were profiled in patients with end-stage renal disease (ESRD, n = 28) or chronic kidney disease (CKD, n = 8) and in healthy (n = 12) subjects by RNA sequencing...
August 7, 2018: International Journal of Cardiology
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