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Endothelial markers

Gurpreet S Wander, Mohammed Yunus Khan Hukkeri, Sachin Yalagudri, Bharti Mahajan, Archana Toppo Panda
Cardiovascular (CV) diseases are a major cause of premature death and disability. Non-communicable diseases (NCD) are responsible for 52% of mortality amongst Indians, of these CV diseases are responsible for 66% of NCD mortality in India. We not only need widespread primary preventive strategy but also need effective secondary prevention protocols to reduce this. Secondary prevention in patients who already had myocardial infarction (MI) or revascularization is of utmost importance to reduce mortality, cardiac events and improve quality of life...
March 2018: Journal of the Association of Physicians of India
Ryan R Kelly, Lindsay T McDonald, Vincent D Pellegrini, James J Cray, Amanda C Larue
BACKGROUND AIMS: Previous studies identified a circulating human osteoblastic population that expressed osteocalcin (OCN), increased following fracture and pubertal growth, and formed mineralized colonies in vitro and bone in vivo. A subpopulation expressed CD34, a hematopoietic/endothelial marker. These findings led to our hypothesis that hematopoietic-derived CD34+ OCN+ cells exist in the circulation of mice and are modulated after fracture. METHODS: Flow cytometry was used to identify CD34+ OCN+ cells in male B6...
October 16, 2018: Cytotherapy
Fang Hou, Yuemeng Dai, Chun-Yang Fan, James Y Suen, Gresham T Richter
BACKGROUND: Estrogen plays a role in infantile hemangioma (IH) development, but the underlying mechanism remains unclear. This study aimed to assess estrogen and estrogen receptor (ER) localization and expression levels in IH. In addition, the unexpected relationship between mast cells (MCs) and estrogen in human IH was discussed. METHODS: IH (n = 29), vascular malformation (VMs, n = 33) and normal skin (n = 15) specimens were assessed. IH was classified into proliferative (n = 9; age, 3...
October 19, 2018: Orphanet Journal of Rare Diseases
Raissa L Davis, Geunho Choi, Thijs Kuiken, Pascale Quéré, Sascha Trapp, Kirsty R Short, Mathilde Richard
BACKGROUND: Endothelial cells play a major role in highly pathogenic avian influenza (HPAI) virus pathogenesis in gallinaceous poultry species (e.g. chicken, turkey and quail). Upon infection of gallinaceous poultry with HPAI viruses, endothelial cells throughout the body become rapidly infected, leading to systemic dissemination of the virus, disseminated intravascular coagulation, oedema and haemorrhaging. In contrast, the pathogenesis of HPAI viruses in most wild bird species (e.g...
October 19, 2018: BMC Microbiology
Xiao-Hua Yu, Lin-Hao He, Jia-Hui Gao, Da-Wei Zhang, Xi-Long Zheng, Chao-Ke Tang
Pregnancy-associated plasma protein-A (PAPP-A), a member of the metzincin metalloproteinase superfamily, can enhance local insulin-like growth factor (IGF) bioavailability through proteolytic cleavage of three IGF binding proteins. In patients with coronary atherosclerosis disease (CAD), elevated PAPP-A levels are significantly associated with a higher risk of cardiovascular events. Accumulating evidence indicates that this protease exerts a proatherogenic effect by altering a variety of pathological processes involved in atherosclerosis, including lipid accumulation, vascular inflammation, endothelial dysfunction, vascular smooth muscle cell proliferation and migration, plaque stability, and thrombus formation...
October 9, 2018: Atherosclerosis
K Krause, R Sabat, E Witte-Händel, A Schulze, V Puhl, M Maurer, K Wolk
BACKGROUND: Schnitzler's syndrome (SchS) is a rare autoinflammatory disease characterized by urticarial exanthema, bone and joint alterations, fever and monoclonal gammopathy, which manifests mostly in the second half of life. It involves over-activation of the interleukin(IL)-1 system, but the exact pathophysiological pathways remain largely unknown. OBJECTIVE: This study aimed at the identification and characterization of pathogenetic players in SchS. METHODS: Blood parameters were quantified in SchS patients compared to healthy subjects and patients with psoriasis and hidradenitis suppurativa using ELISA...
October 19, 2018: British Journal of Dermatology
Rui Adão, Pedro Mendes-Ferreira, Carolina Maia-Rocha, Diana Santos-Ribeiro, Patrícia Gonçalves Rodrigues, André Vidal-Meireles, Cláudia Monteiro-Pinto, Luís D Pimentel, Inês Falcão-Pires, Gilles W De Keulenaer, Adelino F Leite-Moreira, Carmen Brás-Silva
We have previously shown that treatment with recombinant human neuregulin-1 (rhNRG-1) improves pulmonary arterial hypertension (PAH) in a monocrotaline (MCT)-induced animal model, by decreasing pulmonary arterial remodeling and endothelial dysfunction, as well as by restoring RV function. Additionally, rhNRG-1 treatment showed direct myocardial anti-remodelling effects in a model of pressure loading of the RV without PAH. This work aimed to study the intrinsic cardiac effects of rhNRG-1 on experimental PAH and RV pressure overload, and more specifically on diastolic stiffness, at both the ventricular and cardiomyocyte level...
October 19, 2018: Clinical and Experimental Pharmacology & Physiology
Daniele Spinozzi, Alina Miron, Marieke Bruinsma, Isabel Dapena, Itay Lavy, Perry Binder, Mehrdad Rafat, Silke Oellerich, Gerrit R J Melles
Purpose/Aim: Evaluating the suitability of bioengineered collagen sheets and human anterior lens capsules (HALC) as carriers for cultivated porcine corneal endothelial cells (pCEC) and in vitro assessment of the cell-carrier sheets as tissue-engineered grafts for Descemet membrane endothelial keratoplasty (DMEK). MATERIALS AND METHODS: pCEC were isolated, cultured up to P2 and seeded onto LinkCell™ bioengineered matrices of 20µm (LK20) or 100µm (LK100) thickness, and on HALC. During expansion, pCEC viability and morphology were assessed by light microscopy...
October 19, 2018: Current Eye Research
Q-Q Ling, J Zhao, C-G Zheng, J Chun
OBJECTIVE: To investigate the role of the Notch signaling pathway on the endothelial-mesenchymal transition (EndMT) during vascular endothelial dysfunction and atherosclerosis. MATERIALS AND METHODS: Human coronary artery endothelial cells (HCAEC) were treated with the exogenous Notch homolog 1 (Notch1) factor to activate the Notch1 pathway, and cells were then observed under the microscope for morphologic changes. Changes in the expression of related proteins were detected by Western blot...
October 2018: European Review for Medical and Pharmacological Sciences
Dingyuan Tian, Xi Zeng, Weisen Wang, Zhi Wang, Yi Zhang, Yun Wang
Neointima hyperplasia is one of the predominant features of cardiovascular diseases such as atherosclerosis, and is also responsible for the restenosis of vascular surgery including arteriovenous fistula and stent implantation. Endothelial-to-mesenchymal transition (EndMT) contributes to neointima hyperplasia by activation of the Notch or TGF-β signaling pathway. Rapamycin has been utilized as anti-restenosis drug due to its anti-proliferative activity. However, its effects on the EndMT have not been investigated yet...
October 15, 2018: Vascular Pharmacology
N A Solovyeva
The paper describes the procedure to prepare endothelial cell monolayer samples from the vascular intima, which is suitable for studying various morphological processes. To obtain high-quality multicellular specimens, it is recommended to preliminarily remove excess tissue from the outside of the vessel and to free the intima; ways to dry the surface and to separate endothelial cells are examined. The paper gives the figures of specimens stained by the Romanowsky-Giemsa method and identifies factor VIII, an endothelial marker, and Chlamydia pneumoniae inclusions in the cytoplasm of endothelial cells...
2018: Arkhiv Patologii
E A Kogan, N V Zharkov, S I Askolskaya, Yu V Popov, M A Krot, T A Demura
OBJECTIVE: To investigate the growth mechanisms of large uterine leiomyoma (LULM) on the basis of a clinical morphology examination, by providing immunohistochemical (IHC) characteristics of the expression of growth factors (transforming growth factor-beta (TGFβ) and platelet-derived endothelial cell growth factor (PD-ECGF)) and markers of stemness (CD117/c-kit, Connexin 43, Nestin) and proliferation (Ki-67). SUBJECT AND METHODS: The investigators examined surgical specimens from 38 women diagnosed with simple uterine leiomyoma (ULM), who had been divided into two groups: 1) 21 patients with LULM (>6 cm in diameter) (a study group); 2) 17 patients with small ULM (<4 cm in diameter) (a comparison group)...
2018: Arkhiv Patologii
Pavan K Bhatraju, Leila R Zelnick, Jerald Herting, Ronit Katz, Carmen Mikacenic, Susanna Kosamo, Eric D Morrell, Cassianne Robinson-Cohen, Carolyn S Calfee, Jason D Christie, Kathleen D Liu, Michael A Matthay, William O Hahn, Victoria Dmyterko, Natalie S J Slivinski, Jim A Russell, Keith R Walley, David C Christiani, W Conrad Liles, Jonathan Himmelfarb, Mark M Wurfel
OBJECTIVE: The aim of this study was to identify and test whether AKI sub-phenotypes have prognostic and therapeutic implications. METHODS: First, latent class analysis methodology was applied independently in two critically ill populations (Discovery: n=794 and Replication: n=425) with AKI. Second, a parsimonious classification model was developed to identify AKI sub-phenotypes. Third, the classification model was applied to patients with AKI in the Vasopressin in Septic Shock (VASST: n=271) clinical trial and differences in treatment response were determined...
October 18, 2018: American Journal of Respiratory and Critical Care Medicine
Xin Zhao, Yaping Yi, Chao Meng, Ningyuan Fang
Hypertension is a major risk factor for the development of atherosclerosis. Increased carotid intima-media thickness (CIMT) is generally considered as an early marker of atherosclerosis. Recently, circulating microRNAs have been implicated both as sensitive biomarker and key regulator in development of atherosclerosis. However, the biological functions and molecular regulatory mechanisms for miR-575 on angiogenesis remain unknown. In our study, we firstly identified upregulation of circulating miR-575 in plasma of essential hypertensive patients with increased CIMT (iCIMT) compare to those patients with normal CIMT (nCIMT)...
October 17, 2018: Bioscience Reports
Thaís da Silva Ferreira, Julia Freitas Rodrigues Fernandes, Luciene da Silva Araújo, Lívia de Paula Nogueira, Priscila Mansur Leal, Vanessa Parada Antunes, Maria de Lourdes Guimarães Rodrigues, Debora Cristina Torres Valença, Sergio Emanuel Kaiser, Márcia Regina Simas Torres Klein
BACKGROUND: Observational studies have highlighted an association between serum uric acid (SUA) levels and cardiovascular risk factors. Despite the growing body of evidences, several studies were conducted in older individuals or in carriers of diseases susceptible to affect SUA levels and cardiometabolic risk markers. OBJECTIVE: To evaluate the relationship of SUA with body adiposity, metabolic profile, oxidative stress, inflammatory biomarkers, blood pressure and endothelial function in healthy young and middle-aged adults...
October 11, 2018: Arquivos Brasileiros de Cardiologia
Amanda L Patterson, Jitu George, Anindita Chatterjee, Tyler Carpenter, Emily Wolfrum, James K Pru, Jose M Teixeira
Uterine remodeling during pregnancy and postpartum uterine involution are two fundamental, dynamic processes that are required for successful propagation of eutherian species. The uterus can increase in size up to 40-fold during pregnancy, which is largely attributed to expansion of the myometrium by hyperplasia and hypertrophy. Following pregnancy, the uterus repairs the remodeled or "damaged" tissue during uterine involution. Little is known about this repair process, particularly the role of mesenchymal stem/progenitor cells...
October 17, 2018: Stem Cells and Development
Haiyan Zhao, Yaping Zhao, Xin Li, Leiqian Xu, Fangxin Jiang, Wanju Hou, Lixia Dong, Jie Cao
PURPOSE: Obstructive sleep apnea and chronic obstructive pulmonary disease are independent risk factors of cardiovascular disease (CVD), and their coexistence is known as overlap syndrome (OS). Endothelial dysfunction is the initial stage of CVD; however, underlying mechanisms linking OS and CVD are not well understood. The aim of this study was to explore whether OS can lead to more severe inflammation and endothelial apoptosis by promoting endothelial dysfunction, and to assess the intervention effects of antioxidant tempol...
November 2018: Yonsei Medical Journal
Sokratis A Apostolidis, Giuseppina Stifano, Tracy Tabib, Lisa M Rice, Christina M Morse, Bashar Kahaleh, Robert Lafyatis
Objective: The mechanisms that lead to endothelial cell (EC) injury and propagate the vasculopathy in Systemic Sclerosis (SSc) are not well understood. Using single cell RNA sequencing (scRNA-seq), our goal was to identify EC markers and signature pathways associated with vascular injury in SSc skin. Methods: We implemented single cell sorting and subsequent RNA sequencing of cells isolated from SSc and healthy control skin. We used t-distributed stochastic neighbor embedding (t-SNE) to identify the various cell types...
2018: Frontiers in Immunology
Sarah A Wall, Qiuhong Zhao, Martha Yearsley, Luke Blower, Akwasi Agyeman, Parvathi Ranganathan, Shangbin Yang, Haiwa Wu, Matthew Bostic, Samantha Jaglowski, Jonathan E Brammer, Basem William, Hannah Choe, Alice S Mims, Sam Penza, Yvonne Efebera, Steven Devine, Spero Cataland, Stella M Davies, Sumithira Vasu
Transplant-associated thrombotic microangiopathy (TA-TMA), a complication of hematopoietic cell transplant (HCT), is associated with significant morbidity and mortality. The pathophysiology and overlap of TA-TMA with other posttransplant complications such as graft-versus-host disease (GVHD) is poorly understood. We retrospectively identified cases of TA-TMA among patients with grade 3/4 gastrointestinal (GI) GVHD, reviewed intestinal biopsy specimens, and performed correlative testing of biomarkers associated with TA-TMA...
October 23, 2018: Blood Advances
Caroline Sut, Hind Hamzeh-Cognasse, Charles-Antoine Arthaud, Marie-Ange Eyraud, Kamel Chettab, Charles Dumontet, Sandrine Laradi, Thierry Burnouf, Olivier Garraud, Fabrice Cognasse
BACKGROUND: Platelet transfusions are safe but can nevertheless cause serious adverse reactions (SARs). This study investigated the effects of platelet biological response modifiers (BRMs) that accumulate during storage and are commonly associated with transfusion adverse reactions. STUDY DESIGN AND METHODS: Endothelial cells (ECs), that is, EA.hy926, were exposed in vitro to supernatants of platelet components (PCs) that had been either implicated or not in SARs...
October 16, 2018: Transfusion
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