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"neisseria meningitidis"

Thierry Frank, Eva Hong, Jean-Robert Mbecko, Jean-Pierre Lombart, Muhamed-Kheir Taha, Pierre-Alain Rubbo
We analyzed data from the 2015 and 2016 meningitis epidemic seasons in Central African Republic as part of the national disease surveillance. Of 80 tested specimens, 66 belonged to meningococcal serogroup W. Further analysis found that 97.7% of 44 isolates belonged to the hyperinvasive clonal complex sequence type 11.
November 2018: Emerging Infectious Diseases
Aude Garin, Florent Bavozet
Meningococcal pericarditis is a rare but severe form of acute purulent pericarditis. It is a classic complication of Neisseria meningitidis of serotype W135 , usually occurring in pilgrims to Mecca and their household contacts. This severe form of meningococcaemia is difficult to diagnose and evolves frequently and gradually towards a tamponade, requiring emergency drainage. We report a case of meningococcal pericarditis caused by N. meningitidis W135 in an immunocompetent patient, without any other organ involvement especially meningeal, requiring pericardium drainage in emergency and then intrapericardial fibrinolysis...
October 16, 2018: BMJ Case Reports
Jan Silhan, Qiyuan Zhao, Evzen Boura, Hellen Thomson, Andreas Förster, Christoph M Tang, Paul S Freemont, Geoff S Baldwin
NExo is an enzyme from Neisseria meningitidis that is specialized in the removal of the 3'-phosphate and other 3'-lesions, which are potential blocks for DNA repair. NExo is a highly active DNA 3'-phosphatase, and although it is from the class II AP family it lacks AP endonuclease activity. In contrast, the NExo homologue NApe, lacks 3'-phosphatase activity but is an efficient AP endonuclease. These enzymes act together to protect the meningococcus from DNA damage arising mainly from oxidative stress and spontaneous base loss...
October 17, 2018: Nucleic Acids Research
Alessia Liguori, Lucia Dello Iacono, Giulietta Maruggi, Barbara Benucci, Marcello Merola, Paola Lo Surdo, Jacinto López-Sagaseta, Mariagrazia Pizza, Enrico Malito, Matthew J Bottomley
Neisseria meningitidis serogroup B (MenB) is a major cause of sepsis and invasive meningococcal disease. A multicomponent vaccine, 4CMenB, is approved for protection against MenB. Neisserial adhesin A (NadA) is one of the main vaccine antigens, acts in host cell adhesion, and may influence colonization and invasion. Six major genetic variants of NadA exist and can be classified into immunologically distinct groups I and II. Knowledge of the crystal structure of the 4CMenB vaccine component NadA3 (group I) would improve understanding of its immunogenicity, folding, and functional properties and might aid antigen design...
October 16, 2018: MBio
F Laurent, J Pingitore, C Rossi
Neisseria meningitidis is the cause of relatively rare invasive infections, but with very important morbi-mortality. Vaccines are already available for serogroups A, C, W, Y. A new vaccine against meningococcus B is on the Belgian market since March 2017. Serogroup B is the most prevalent in Western countries. The target populations are children and teenager, since it is at this age that peaks in the incidence of meningococcal disease are observed. The development of this vaccine has been made possible by a new process named " reverse vaccinology ", which uses the complete genome sequencing of the bacterium...
2018: Revue Médicale de Bruxelles
Evelína Kánová, Irene Jiménez-Munguía, Petra Majerová, Zuzana Tkáčová, Katarína Bhide, Patrícia Mertinková, Lucia Pulzová, Andrej Kováč, Mangesh Bhide
Neisseria meningitidis is able to translocate the blood-brain barrier and cause meningitis. Bacterial translocation is a crucial step in the onset of neuroinvasion that involves interactions between pathogen surface proteins and host cells receptors. In this study, we applied a systematic workflow to recover and identify proteins of N. meningitidis that may interact with human brain microvascular endothelial cells (hBMECs). Biotinylated proteome of N. meningitidis was incubated with hBMECs, interacting proteins were recovered by affinity purification and identified by SWATH-MS...
2018: Frontiers in Microbiology
Joanna L Hicks, Claire V Mullholland
The principal mechanism of reducing sulfur into organic compounds is via the synthesis of l-cysteine. Cysteine is used for protein and glutathione synthesis, as well as being the primary sulfur source for a variety of other molecules, such as biotin, coenzyme A, lipoic acid and more. Glutathione and other cysteine derivatives are important for protection against the oxidative stress that pathogenic bacteria such as Neisseria gonorrhoeae and Neisseria meningitidis encounter during infection. With the alarming rise of antibiotic-resistant strains of N...
October 11, 2018: Microbiology
Stéphane Emonet, Rita Born, Jacques Schrenzel
In the last decade, the epidemiology of meningococcal infections in Switzerland and Europe has changed. The prevalence of Y and W serogroups augmented, with more atypical clinical manifestations such as pharyngitis, pneumonia and arthritis. A hypervirulent clonal serogroup W strain of Neisseria meningitidis has emerged in Europe and Switzerland, with a noticeable potential for epidemics as evidenced by its clonality. In this context, vaccinal recommendations for risk groups have and will continue to evolve towards the use of novel conjugated vaccines...
October 10, 2018: Revue Médicale Suisse
Hannia Liliana Almonacid-Mendoza, María Victoria Humbert, Aiste Dijokaite, David W Cleary, Yiwen Soo, Miao-Chiu Hung, Christian M Orr, Moritz M Machelett, Ivo Tews, Myron Christodoulides
Neisseria gonorrhoeae (gonococcus [Ng]) is the causative organism of the sexually transmitted disease gonorrhoea, and no effective vaccine exists currently. In this study, the structure, biological properties, and vaccine potential of the Ng-adhesin complex protein (Ng-ACP) are presented. The crystal structure of recombinant Ng-ACP (rNg-ACP) protein was solved at 1.65 Å. Diversity and conservation of Ng-ACP were examined in different Neisseria species and gonococcal isolates ( database) in silico , and protein expression among 50 gonococcal strains in the Centers for Disease Control and Prevention/Food and Drug Administration (CDCP/FDA) AR Isolate Bank was examined by Western blotting...
October 10, 2018: MSphere
Robert Booy, Angela Gentile, Michael Nissen, Jane Whelan, Véronique Abitbol
Invasive meningococcal disease (IMD) is a serious disease that is fatal in 5-15% and disabling in 12-20% of cases. The dynamic and unpredictable epidemiology is a particular challenge of IMD prevention. Although vaccination against meningococcal serogroups A (MenA), MenC and, more recently, MenB, are proving successful, other serogroups are emerging as major IMD causes. Recently, surges in MenW incidence occurred in South America, Europe, Australia and parts of sub-Saharan Africa, with hypervirulent strains being associated with severe IMD and higher fatality rates...
October 8, 2018: Human Vaccines & Immunotherapeutics
Raymond S W Tsang, Tauqeer Ahmad, Frances B Jamieson, Gregory J Tyrrell
Objectives: Neisseria meningitidis is rarely penicillin resistant. We describe WGS analysis of a penicillin-resistant N. meningitidis collected from a case of invasive meningococcal disease. Methods: Serogrouping, serotyping and serosubtyping were performed with specific antibodies. β-Lactamase was detected by nitrocefin. MICs were determined by Etest and agar dilution. Sequencing of N. meningitidis genomes was done on the Illumina MiSeq platform and genome data were analysed using the Bacterial Isolate Genome Sequence Database (BIGSdb) on the PubMLST Neisseria website (https://pubmlst...
October 8, 2018: Journal of Antimicrobial Chemotherapy
Luke P Dawson, James Hare, Stephen J Duffy
Cardiac involvement can occur following bacteremia secondary to Neisseria meningitidis (Garcia et al. Rev Soc Bras Med Trop. 1999;32(5):517-5122) and is likely underrecognized. However, meningococcal disease presenting as primary myopericarditis in immunocompetent adults is a relatively rare phenomenon. We discuss an unusual presentation of meningococcal disease and the investigation and treatment strategies implemented.
November 2018: Diagnostic Microbiology and Infectious Disease
Eva Hong, Younes Bakhalek, Muhamed-Kheir Taha
OBJECTIVES: The Matrix Laser Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF/MS) technique is increasingly used in hospital laboratories for routine identification of microorganisms. However, its performance is variable particularly for highly variable species such as Neisseria meningitidis. Reliable identification of N. meningitidis is crucial for the management of invasive meningococcal disease by rapid implementation of treatment and preventive measures among close contacts...
October 1, 2018: Clinical Microbiology and Infection
Matthias J H Gerritzen, Ronald H W Maas, Jan van den Ijssel, Lonneke van Keulen, Dirk E Martens, René H Wijffels, Michiel Stork
BACKGROUND: Outer membrane vesicles (OMVs) are nanoparticles released by Gram-negative bacteria and can be used as vaccines. Often, detergents are used to promote release of OMVs and to remove the toxic lipopolysaccharides. Lipopolysaccharides can be detoxified by genetic modification such that vesicles spontaneously produced by bacteria can be directly used as vaccines. The use of spontaneous OMVs has the advantage that no separate extraction step is required in the purification process...
October 3, 2018: Microbial Cell Factories
Soheila Hekmat, Seyed Mehdi Sadat, Mohammad Mehdi Aslani, Mehdi Mahdavi, Azam Bolhassani, Fateme Asgar Halvaee, Seyed Mohammad Mahdi Ghahari, Mohammad Reza Aghasadeghi, Seyed Davar Siadat
Background: A licensed vaccine against hepatitis C virus (HCV) has not become available to date. The stability and antigenicity of a targeted synthesized recombinant fusion protein consisting of a truncated core and NS3 (rC/N) of HCV had been predicted. Although safe antigens, recombinant proteins are not efficacious vaccines without adjuvants. The present study evaluated the immunogenicity of rC/N as a bipartite antigen accompanied by Neisseria meningitidis serogroup B outer membrane vesicles (NMB OMVs) in BALB/c mice...
October 3, 2018: Iranian Biomedical Journal
Sandrine Boschi-Muller
Neisseria meningitidis , an obligate pathogenic bacterium in humans, has acquired different defense mechanisms to detect and fight the oxidative stress generated by the host's defense during infection. A notable example of such a mechanism is the PilB reducing system, which repairs oxidatively-damaged methionine residues. This review will focus on the catalytic mechanism of the two methionine sulfoxide reductase (MSR) domains of PilB, which represent model enzymes for catalysis of the reduction of a sulfoxide function by thiols through sulfenic acid chemistry...
October 1, 2018: Antioxidants (Basel, Switzerland)
Cynthia Burman, Lidia Serra, Charles Nuttens, Jessica Presa, Paul Balmer, Laura York
Invasive meningococcal disease (IMD) caused by Neisseria meningitidis is characterized by high mortality and morbidity. While IMD incidence peaks in both infants and adolescents/young adults, carriage rates are often highest in the latter age groups, increasing IMD risk and the likelihood of transmission. Effective vaccines are available for 5 of 6 disease-causing serogroups. Because adolescents/young adults represent a significant proportion of cases, often have the highest carriage rate, and have characteristically low vaccination adherence, efforts should be focused on educating this population regarding long-term consequences of infection and the importance of meningococcal vaccination in prevention...
October 1, 2018: Human Vaccines & Immunotherapeutics
Alessandra Mozzi, Diego Forni, Mario Clerici, Rachele Cagliani, Manuela Sironi
Iron is an essential micronutrient for most living species. In mammals, hemoglobin (Hb) stores more than two thirds of the body's iron content. In the bloodstream, haptoglobin (Hp) and hemopexin (Hpx) sequester free Hb or heme. Pathogenic microorganisms usually acquire iron from their hosts and have evolved complex systems of iron piracy to circumvent nutritional immunity. Herein, we performed an evolutionary analysis of genes coding for mammalian heme-binding proteins and heme-scavengers in pathogen species...
2018: Frontiers in Immunology
Shannon L Harris, Cuiwen Tan, Lubomira Andrew, Li Hao, Paul A Liberator, Judith Absalon, Annaliesa S Anderson, Thomas R Jones
MenB-FHbp (Trumenba®; bivalent rLP2086) is a meningococcal serogroup B vaccine containing 2 variants of the recombinant lipidated factor H binding protein (FHbp) antigen. The expression of FHbp, an outer membrane protein, is not restricted to serogroup B strains of Neisseria meningitidis (MenB). This study investigated whether antibodies elicited by MenB-FHbp vaccination also protect against non-MenB strains. Immunological responses were assessed in serum bactericidal assays using human complement (hSBAs) with non-MenB disease-causing test strains from Europe, Africa, and the United States...
September 27, 2018: Vaccine
Kerstin J Olbrich, Dirk Müller, Sarah Schumacher, Ekkehard Beck, Kinga Meszaros, Florian Koerber
INTRODUCTION: Invasive meningococcal disease (IMD, septicaemia and/or meningitis) has a severe acute and long-term burden: 5-10% of patients die within 48 h, and long-term sequelae have been reported in 10-20% of survivors. Health-related quality of life (HRQoL) is increasingly but inconsistently assessed. METHODS: A systematic literature review on Neisseria meningitidis IMD sequelae and HRQoL in survivors of all ages and their caregivers, including family, was conducted for high-income countries from 2001 to 2016 (in Medline and Embase, following Cochrane and PRISMA guidelines)...
September 28, 2018: Infectious Diseases and Therapy
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