keyword
MENU ▼
Read by QxMD icon Read
search

Biofilm formation

keyword
https://www.readbyqxmd.com/read/28738346/synergistic-activity-of-berberine-with-azithromycin-against-pseudomonas-aeruginosa-isolated-from-patients-with-cystic-fibrosis-of-lung-in-vitro-and-in-vivo
#1
YongTao Li, JianRong Huang, LanJuan Li, LinSheng Liu
BACKGROUND/AIMS: Pseudomonas aeruginosa (PA) is one of the major opportunistic pathogens which can cause chronic lung infection of cystic fibrosis (CF). The formation of PA biofilm promotes CF development and restricts the antimicrobial efficacies of current antibiotics. METHODS: The antimicrobial effects of azithromycin (AZM) and berberine (BER) alone and in combination were evaluated using microdilution method, checkerboard assay, time-kill test, qRT-PCR analysis and absorption method...
July 24, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28738195/proteomic-and-genetics-insights-on-the-response-of-the-bacteriocinogenic-lactobacillus-sakei-crl1862-during-biofilm-formation-on-stainless-steel-surface-at-10%C3%A2-c
#2
Mariana Pérez-Ibarreche, Lucía M Mendoza, Graciela Vignolo, Silvina Fadda
Some lactic acid bacteria have the ability to form biofilms on food-industry surfaces and this property could be used to control food pathogens colonization. Lactobacillus sakei CR1862 was selected considering its bacteriocinogenic nature and ability to adhere to abiotic surfaces at low temperatures. In this study, the proteome of L. sakei CRL1862 grown either under biofilm on stainless steel surface and planktonic modes of growth at 10°C, was investigated. Using two-dimensional gel electrophoresis, 29 out of 43 statistically significant spots were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry...
July 4, 2017: International Journal of Food Microbiology
https://www.readbyqxmd.com/read/28737897/biogenic-hydroxyapatite-a-new-material-for-the-preservation-and-restoration-of-the-built-environment
#3
Ronald Joseph Turner, Joanna C Renshaw, Andrea Hamilton
Ordinary Portland Cement (OPC) is by weight the world's most produced man-made material, and consequently is used in a variety of applications in environments ranging from buildings, to nuclear wasteforms, and within the human body. In this paper, we present for the first time the direct deposition of biogenic hydroxyapatite onto the surface of OPC, in a synergistic process which relies upon the unique properties of the cement substrate. The synthesised hydroxyapatite is very similar to that found in nature; having a crystallite size, iron and carbonate substitution, and a semi-crystalline structure which identify that this biogenic hydroxyapatite is akin to that found in natural bone and tooth enamel...
July 24, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28737725/optimization-of-the-silver-nanoparticles-peald-process-on-the-surface-of-1-d-titania-coatings
#4
Aleksandra Radtke, Tomasz Jędrzejewski, Wiesław Kozak, Beata Sadowska, Marzena Więckowska-Szakiel, Ewa Talik, Maarit Mäkelä, Markku Leskelä, Piotr Piszczek
Plasma enhanced atomic layer deposition (PEALD) of silver nanoparticles on the surface of 1-D titania coatings, such as nanotubes (TNT) and nanoneedles (TNN), has been carried out. The formation of TNT and TNN layers enriched with dispersed silver particles of strictly defined sizes and the estimation of their bioactivity was the aim of our investigations. The structure and the morphology of produced materials were determined using X-ray photoelectron spectroscopy (XPS) and scanning electron miscroscopy (SEM)...
July 24, 2017: Nanomaterials
https://www.readbyqxmd.com/read/28737700/a-novel-brominated-alkaloid-securidine-a-isolated-from-the-marine-bryozoan-securiflustra-securifrons
#5
Priyanka Michael, Kine Ø Hansen, Johan Isaksson, Jeanette H Andersen, Espen Hansen
A novel brominated alkaloid, Securidine A, was isolated from the cold water marine bryozoan Securiflustra securifrons. Securidine A was isolated using semi-preparative HPLC, and the structure was elucidated by spectroscopic methods. The isolated Securidine A was tested for cytotoxic, antibacterial, and anti-diabetic activities as well as for its potential for inhibition of biofilm formation. No significant biological activity was observed in the applied bioassays, thus expanded bioactivity profiling is required, in order to reveal any potential applications for Securidine A...
July 23, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/28736865/bioprotection-of-the-built-environment-and-cultural-heritage
#6
Geoffrey Michael Gadd, Thomas D Dyer
The growth of microbial biofilms and various biomineralization phenomena can lead to the formation of stable layers and veneers on rocks known as 'rock varnishes' that can stabilize surfaces and protect from further weathering. This article describes the potential application of fungal systems for bioprotection of rock and mineral-based substrates and the evidence to support this concept of utilizing natural or engineered colonization and metabolic properties of fungi, including lichens.
July 24, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28736523/sub-minimum-inhibitory-concentrations-of-rhubarb-water-extracts-inhibit-streptococcus-suis-biofilm-formation
#7
Wen-Ya Ding, Yan-Hua Li, He Lian, Xiao-Yu Ai, Yu-Lin Zhao, Yan-Bei Yang, Qiang Han, Xin Liu, Xue-Ying Chen, Zhonggui He
Streptococcus suis is one of the most important swine pathogens, which can cause persistent infection by forming biofilms. In this study, sub-minimum inhibitory concentration (sub-MIC) of rhubarb water extracts were found to inhibit biofilm formation. Two-component signal transduction systems (TCSs), transcriptional regulators, and DNA binding proteins were compared under two conditions: (1) cells treated with sub-MIC rhubarb water extracts and (2) untreated cells. Using an isobaric tags for relative and absolute quantitation (iTRAQ) strategy, we found that TCSs constituent proteins of histidine kinase and response regulator were significantly down-regulated...
2017: Frontiers in Pharmacology
https://www.readbyqxmd.com/read/28735852/roles-of-quorum-sensing-molecules-from-rhizobium-etli-rt1-in-bacterial-motility-and-biofilm-formation
#8
Swarnita Dixit, Ramesh Chand Dubey, Dinesh Kumar Maheshwari, Prahlad Kishore Seth, Vivek K Bajpai
Strain RT1 was isolated from root nodules of Lens culinaris (a lentil) and characterized as Rhizobium etli (a Gram-negative soil-borne bacterium) by 16S rDNA sequencing and phylogenetic analysis. The signaling molecules produced by R. etli (RT1) were detected and identified by high-performance liquid chromatography coupled with mass spectrometry. The most abundant and biologically active N-acyl homoserine lactone molecules (3-oxo-C8-HSL and 3-OH-C14-HSL) were detected in the ethyl acetate extract of RT1. The biological role of 3-oxo-C8-HSL was evaluated in RT1...
July 8, 2017: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/28735329/antistaphylococcal-activity-of-selected-thiourea-derivatives
#9
Joanna Stefańska, Karolina Stępień, Anna Bielenica, Małgorzata Wrzosek, Marta Struga
Five of thiourea derivatives were prepared using as a starting compound 3-(trifluoromethyl)aniline, 4-chloro-3-nitroaniline, 1,3-thiazol-2-amine, 2H-1,2,3-triazol-4-amine and commercial isothiocyanates. All compounds were evaluated in vitro for antimicrobial activity. Derivatives 2 and 3 showed the highest inhibition against Gram-positive cocci (standard and hospital strains). The observed MIC values were in the range of 0.5-8 μg/ml. The products effectively inhibited the formation of biofilms of methicillin-resistant and standard strains of Staphylococcus epidermidis...
January 2, 2017: Polish Journal of Microbiology
https://www.readbyqxmd.com/read/28735047/sesamol-exhibits-potent-antimycobacterial-activity-underlying-mechanisms-and-impact-on-virulence-traits
#10
Sandeep Hans, Sharda Sharma, Saif Hameed, Zeeshan Fatima
OBJECTIVES: Novel strategies to overcome multidrug resistance (MDR) in Tuberculosis (TB) still remain a concern. Usage of natural compounds nowadays to surmount the increasing burden of MDR-TB has shown promising results. The aim of this study was to evaluate the antimycobacterial potential of sesamol (Ses) a natural phenolic compound against Mycobacterium smegmatis, a surrogate for MTB and its underlying mechanism of action along with its effect on mycobacterial virulence traits. METHODS: Cell surface phenotypes were estimated microscopically and spectrophotometrically respectively...
July 19, 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28733286/the-regulator-ldhr-and-the-d-lactate-dehydrogenase-ldha-of-burkholderia-multivorans-play-a-role-in-carbon-overflow-and-in-planktonic-cellular-aggregates-formation
#11
Inês N Silva, Marcelo J Ramires, Lisa A Azevedo, Ana R Guerreiro, Andreia C Tavares, Jörg D Becker, Leonilde M Moreira
LysR-type transcriptional regulators (LTTR) are the most commonly found regulators in Burkholderia cepacia complex, comprising opportunistic pathogens causing chronic respiratory infections in cystic fibrosis (CF) patients. Despite LTTRs being global regulators of pathogenicity in several bacteria, few have been characterized in Burkholderia Here, we showed that gene ldhR of B. multivorans encoding a LTTR is co-transcribed with ldhA encoding a d-lactate dehydrogenase, and evaluate their implication in virulence traits like exopolysaccharide (EPS) synthesis and biofilm formation...
July 21, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28732564/desiccation-and-ethanol-resistances-of-multidrug-resistant-acinetobacter-baumannii-embedded-in-biofilm-the-favorable-antiseptic-efficacy-of-combination-chlorhexidine-gluconate-and-ethanol
#12
Shyh-Ren Chiang, Fang Jung, Hung-Jen Tang, Chung-Hua Chen, Chi-Chung Chen, Hsiu-Yin Chou, Yin-Ching Chuang
BACKGROUND/PURPOSE: Globally, multidrug-resistant Acinetobacter baumannii (MDRAB) has emerged as an important pathogen in nosocomial outbreaks. This study aimed to investigate the correlation between the biofilm formation and survival of MDRABs, and to investigate the antiseptic efficacy of hand sanitizers for the MDRABs, embedded with biofilm (MDRAB-Bs). METHODS: The MDRABs were selected randomly after pulsed-field gel electrophoresis (PFGE), and their biofilm formation was analyzed...
June 28, 2017: Journal of Microbiology, Immunology, and Infection, Wei Mian Yu Gan Ran za Zhi
https://www.readbyqxmd.com/read/28732463/common-and-phylogenetically-widespread-coding-for-peptides-by-bacterial-small-rnas
#13
Robin C Friedman, Stefan Kalkhof, Olivia Doppelt-Azeroual, Stephan A Mueller, Martina Chovancová, Martin von Bergen, Benno Schwikowski
BACKGROUND: While eukaryotic noncoding RNAs have recently received intense scrutiny, it is becoming clear that bacterial transcription is at least as pervasive. Bacterial small RNAs and antisense RNAs (sRNAs) are often assumed to be noncoding, due to their lack of long open reading frames (ORFs). However, there are numerous examples of sRNAs encoding for small proteins, whether or not they also have a regulatory role at the RNA level. METHODS: Here, we apply flexible machine learning techniques based on sequence features and comparative genomics to quantify the prevalence of sRNA ORFs under natural selection to maintain protein-coding function in 14 phylogenetically diverse bacteria...
July 21, 2017: BMC Genomics
https://www.readbyqxmd.com/read/28731846/presence-and-future-of-culturing-bacteria
#14
Jörg Overmann, Birte Abt, Johannes Sikorski
The cultivation of bacteria is highly biased toward a few phylogenetic groups. Many of the currently underexplored bacterial lineages likely have novel biosynthetic pathways and unknown biochemical features. New cultivation concepts have been developed based on an improved understanding of the ecology of previously not-cultured bacteria. Particularly successful were improved media that mimic the natural types and concentrations of substrates and nutrients, high-throughput cultivation techniques, and approaches that exploit biofilm formation and bacterial interactions...
July 21, 2017: Annual Review of Microbiology
https://www.readbyqxmd.com/read/28731758/noncontact-cohesive-swimming-of-bacteria-in-two-dimensional-liquid-films
#15
Ye Li, He Zhai, Sandra Sanchez, Daniel B Kearns, Yilin Wu
Bacterial swimming in confined two-dimensional environments is ubiquitous in nature and in clinical settings. Characterizing individual interactions between swimming bacteria in 2D confinement will help to understand diverse microbial processes, such as bacterial swarming and biofilm formation. Here we report a novel motion pattern displayed by flagellated bacteria in 2D confinement: When two nearby cells align their moving directions, they tend to engage in cohesive swimming without direct cell body contact, as a result of hydrodynamic interaction but not flagellar intertwining...
July 7, 2017: Physical Review Letters
https://www.readbyqxmd.com/read/28731279/influence-of-l-amino-acids-on-aggregation-and-biofilm-formation-in-azotobacter-chroococcum-and-trichoderma-viride
#16
Velmourougane Kulandaivelu, Radha Prasanna
AIM: The effects of L-amino acids on growth and biofilm formation in Azotobacter chroococcum (Az) and Trichoderma viride (Tv) as single (Az, Tv) and staggered inoculated cultures (Az-Tv, Tv-Az) was investigated. METHODS AND RESULTS: A preliminary study using a set of twenty L-amino acids, identified six amino acids (L-Glu, L-Gln, L-His, L-Ser, L-Thr, and L-Trp) which significantly enhanced growth and biofilm formation. Supplementation of these amino acids at different concentrations revealed that 40 mmol l(-1) was most effective...
July 21, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28731269/biofilm-formation-and-antibiotic-resistance-in-klebsiella-pneumoniae-urinary-strains
#17
Claudia Vuotto, Francesca Longo, Chiara Pascolini, Gianfranco Donelli, Maria Pia Balice, Maria Francesca Libori, Valentina Tiracchia, Antonino Salvia, Pietro Emanuele Varaldo
AIMS: Multi-drug resistant Klebsiella pneumoniae has become a relevant healthcare-associated pathogen. Capsule, type 1 and 3 fimbriae (mrkA gene), type 2 quorum-sensing system (luxS), synthesis of D-galactan I (wbbM), LPS transport (wzm) and poly-beta-1,6-N-acetyl-D-glucosamine (pgaA) seem involved in K. pneumoniae biofilm. Non-enzymatic antibiotic resistance is related to non-expression or mutation of porins (OmpK35 and OmpK36), and efflux pump (acrB) over-expression. The aim of this study was to analyse some virulence factors of K...
July 21, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28730700/relating-switching-rates-between-normal-and-persister-cells-to-substrate-and-antibiotic-concentrations-a-mathematical-modelling-approach-supported-by-experiments
#18
Gabriel Carvalho, Cyril Guilhen, Damien Balestrino, Christiane Forestier, Jean-Denis Mathias
We developed and compared two mathematical models of variable phenotypic switching rates between normal and persister cells that depend on substrate concentration and antibiotic presence. They could be used to simulate the formation of persisters in environments with concentration gradients such as biofilms. Our models are extensions of a previous model of the dynamics of normal and persistent cell populations developed by Balaban et al. (2004, Science 305: 1622). We calibrated the models' parameters with experimental killing curves obtained after ciprofloxacin treatment of samples regularly harvested from planktonic batch cultures of Klebsiella pneumoniae...
July 21, 2017: Microbial Biotechnology
https://www.readbyqxmd.com/read/28730269/emergence-of-candida-glabrata-in-vulvovaginal-candidiasis-should-be-attributed-to-selective-pressure-or-virulence-ability
#19
Sandra Sayuri Nakamura-Vasconcelos, Adriana Fiorini, Pamela Delvas Zanni, Patrícia de Souza Bonfim-Mendonça, Janine Ribeiro Godoy, Adriana Araújo Almeida-Apolonio, Marcia Edilaine Lopes Consolaro, Terezinha Inez Estivalet Svidzinski
PURPOSE: Vulvovaginal candidiasis (VVC) is one of the most frequent female genital disorders and Candida glabrata is the second most common agent. Current study was aimed to study the susceptibility to antifungal agents of C. glabrata isolated from vaginal samples and some virulence attributes in order to better understand why this species is emerging as the main VVC agents. METHODS: A total of 60 C. glabrata vaginal isolates were included in this study. Firstly they were screened by susceptibility tests to antifungal agents...
July 20, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/28729722/structure-based-discovery-of-small-molecule-inhibitors-of-cariogenic-virulence
#20
Qiong Zhang, Bhavitavya Nijampatnam, Zhang Hua, Thao Nguyen, Jing Zou, Xia Cai, Suzanne M Michalek, Sadanandan E Velu, Hui Wu
Streptococcus mutans employs a key virulence factor, three glucosyltransferase (GtfBCD) enzymes to establish cariogenic biofilms. Therefore, the inhibition of GtfBCD would provide anti-virulence therapeutics. Here a small molecule library of 500,000 small molecule compounds was screened in silico against the available crystal structure of the GtfC catalytic domain. Based on the predicted binding affinities and drug-like properties, small molecules were selected and evaluated for their ability to reduce S. mutans biofilms, as well as inhibit the activity of Gtfs...
July 20, 2017: Scientific Reports
keyword
keyword
16825
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"