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Biofilm formation

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https://www.readbyqxmd.com/read/28647798/a-new-model-for-biofilm-formation-and-inflammatory-tissue-reaction-intraoperative-infection-of-a-cranial-implant-with-staphylococcus-aureus-in-rats
#1
Silke Glage, Silke Paret, Andreas Winkel, Meike Stiesch, André Bleich, Joachim K Krauss, Kerstin Schwabe
BACKGROUND: Implant failure is a severe and frequent adverse event in all areas of neurosurgery. It often involves infection with biofilm formation, accompanied by inflammation of surrounding tissue, including the brain, and bone loss. The most common bacteria involved are Staphylococcus aureus. We here test whether intraoperative infection of intracranial screws with Staphylococcus aureus would lead to biofilm formation and inflammatory tissue reaction in rats. METHODS: Two titanium screws were implanted in the cranium of Sprague-Dawley rats, anesthetized with xylazine (4 mg/kg) and ketamine (75 mg/kg)...
June 25, 2017: Acta Neurochirurgica
https://www.readbyqxmd.com/read/28647320/an-integrated-aerobic-anaerobic-strategy-for-performance-enhancement-of-pseudomonas-aeruginosa-inoculated-microbial-fuel-cell
#2
Xiao-Yu Yong, Zhi-Ying Yan, Hai-Bo Shen, Jun Zhou, Xia-Yuan Wu, Li-Juan Zhang, Tao Zheng, Min Jiang, Ping Wei, Hong-Hua Jia, Yang-Chun Yong
Microbial fuel cell (MFC) is a promising device for energy generation and organic waste treatment simultaneously by electrochemically active bacteria (EAB). In this study, an integrated aerobic-anaerobic strategy was developed to improve the performance of P. aeruginosa-inoculated MFC. With an aerobic start-up and following an anaerobic discharge process, the current density of MFC reached a maximum of 99.80µA/cm(2), which was 91.6% higher than the MFC with conventional constant-anaerobic operation. Cyclic voltammetry and HPLC analysis showed that aerobic start-up significantly increased electron shuttle (pyocyanin) production (76% higher than the constant-anaerobic MFC)...
June 12, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28647124/putative-protein-vc0395_0300-from-vibrio-cholerae-is-a-diguanylate-cyclase-with-a-role-in-biofilm-formation
#3
Divya Bandekar, Om Prakash Chouhan, Swati Mohapatra, Mousumi Hazra, Saugata Hazra, Sumit Biswas
The hallmark of the lifecycle of Vibrio cholerae is its ability to switch between two lifestyles - the sessile, non-pathogenic form and the motile, infectious form in human hosts. One of these changes is in the formation of surface biofilms, when in sessile aquatic habitats. The cell-cell interactions within a V. cholerae biofilm are stabilized by the production of an exopolysachharide (EPS) matrix, which in turn is regulated by the ubiquitous secondary messenger, cyclic di-GMP (c-di-GMP), synthesized by proteins containing GGD(/E)EF domains in all prokaryotic systems...
September 2017: Microbiological Research
https://www.readbyqxmd.com/read/28647025/olumacostat-glasaretil-a-promising-topical-sebum-suppressing-agent-that-affects-all-major-pathogenic-factors-of-acne-vulgaris
#4
Bodo C Melnik
Hunt et al. show that olumacostat glasaretil, an inhibitor of acetyl coenzyme A carboxylase, reduces saturated and monounsaturated fatty acyl chains in sebaceous lipids. Topical olumacostat glasaretil application decreases hamster ear sebaceous gland size and shows efficacy in treating patients with acne vulgaris. Olumacostat glasaretil-mediated sebum suppression may reduce Propionibacterium acnes growth and biofilm formation, comedogenesis, and inflammation.
July 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28646205/comparative-nanouplc-ms-e-analysis-between-magainin-i-susceptible-and-resistant-escherichia-coli-strains
#5
Marlon H Cardoso, Keyla C de Almeida, Elizabete de S Cândido, André M Murad, Simoni C Dias, Octávio L Franco
In recent years the antimicrobial peptides (AMPs) have been prospected and designed as new alternatives to conventional antibiotics. Indeed, AMPs have presented great potential toward pathogenic bacterial strains by means of complex mechanisms of action. However, reports have increasingly emerged regarding the mechanisms by which bacteria resist AMP administration. In this context, we performed a comparative proteomic study by using the total bacterial lysate of magainin I-susceptible and -resistant E. coli strains...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28644912/biofilm-formation-and-disinfectant-resistance-of-salmonella-spp-in-mono-and-dual-species-with-pseudomonas-aeruginosa
#6
Xinyi Pang, Yishan Yang, Hyun-Gyun Yuk
AIMS: This study aimed to evaluate the biofilm formation and disinfectant resistance of Salmonella cells in mono- and dual-species biofilms with Pseudomonas aeruginosa, and to investigate the role of extracellular polymeric substances (EPS) in protection of biofilms against disinfection treatment. METHODS AND RESULTS: The populations of Salmonella in mono- or dual-species biofilms with P. aeruginosa on stainless steel (SS) coupons were determined before and after exposure to commercial disinfectant, 50 μg ml(-1) chlorine or 200 μg ml(-1) Ecolab(®) Whisper(™) V (a blend of four effective quaternary ammonium compounds)...
June 23, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28643906/in-vitro-evaluation-of-surface-properties-of-pro-seal-%C3%A2-and-opal-%C3%A2-seal-tm-in-preventing-white-spot-lesions
#7
T S Premaraj, N Rohani, D Covey, S Premaraj
OBJECTIVES: To evaluate the surface properties of two commercially available sealants (Pro Seal(®) (PS) and Opal(®) Seal(TM) (OS)) in terms of fluoride(F) release, biofilm formation of Streptococcus mutans and Lactobacillus and the ability to resist acid penetration. SETTING: University of Nebraska Medical Center. MATERIAL & METHODS: Discs of similar diameter and thickness were made from OS and PS. Discs were soaked in double-distilled water, and F released was measured with fluoride meter daily for 14 consecutive days, then at 21 and 28 days...
June 2017: Orthodontics & Craniofacial Research
https://www.readbyqxmd.com/read/28643413/effects-of-sodium-chloride-on-heat-resistance-oxidative-susceptibility-motility-biofilm-and-plaque-formation-of-burkholderia-pseudomallei
#8
Pornpan Pumirat, Muthita Vanaporn, Usa Boonyuen, Nitaya Indrawattana, Amporn Rungruengkitkun, Narisara Chantratita
Burkholderia pseudomallei is an environmental saprophyte and the causative agent of melioidosis, a severe infectious disease prevalent in tropical areas, including southeast Asia and northern Australia. In Thailand, the highest incidence of melioidosis is in the northeast region, where saline soil and water are abundant. We hypothesized that B. pseudomallei develops an ability to thrive in saline conditions and gains a selective ecological advantage over other soil-dwelling microorganisms. However, little is known about how an elevated NaCl concentration affects survival and adaptive changes in this pathogen...
June 23, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/28643095/-local-antibiotic-therapy
#9
REVIEW
Klaus-Dieter Kühn, Nora Renz, Andrej Trampuz
The treatment of superficial wound infections with local antibiotics is considered to be problematic as it is accompanied by an increased risk of resistance development and ineffectiveness in deeper regions. On the contrary, the local application of antibiotics is useful in arthroplasty and necessary if implants are utilized. The reason for this is the prevention of a rapid biofilm formation on foreign implants and a resulting infection may remain undiscovered until it becomes chronic. Apart from exogenous material, necrotic tissue (e...
June 22, 2017: Der Unfallchirurg
https://www.readbyqxmd.com/read/28642776/regulation-involved-in-colonization-of-intercellular-spaces-of-host-plants-in-ralstonia-solanacearum
#10
REVIEW
Yasufumi Hikichi, Yuka Mori, Shiho Ishikawa, Kazusa Hayashi, Kouhei Ohnishi, Akinori Kiba, Kenji Kai
A soil-borne bacterium Ralstonia solanacearum invading plant roots first colonizes the intercellular spaces of the root, and eventually enters xylem vessels, where it replicates at high levels leading to wilting symptoms. After invasion into intercellular spaces, R. solanacearum strain OE1-1 attaches to host cells and expression of the hrp genes encoding components of the type III secretion system (T3SS). OE1-1 then constructs T3SS and secrets effectors into host cells, inducing expression of the host gene encoding phosphatidic acid phosphatase...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28642753/suhb-regulates-the-motile-sessile-switch-in-pseudomonas-aeruginosa-through-the-gac-rsm-pathway-and-c-di-gmp-signaling
#11
Kewei Li, Guangjian Yang, Alexander B Debru, Pingping Li, Li Zong, Peizhen Li, Teng Xu, Weihui Wu, Shouguang Jin, Qiyu Bao
Many Pseudomonas aeruginosa virulence traits that contribute to human infections are accepted as being associated with its environmental lifestyle. Therefore, identifying the molecular mechanisms that govern the lifestyle choice is of high significance. We previously reported that a mutation in suhB results in a decrease in swimming motility and increased biofilm formation compared to the wild-type strain. Yet, little is known about how this occurs. In this study, we demonstrated that SuhB inversely regulates motility and biofilm formation through the GacA-RsmY/Z-RsmA cascade...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28642749/rna-seq-reveals-enhanced-sugar-metabolism-in-streptococcus-mutans-co-cultured-with-candida-albicans-within-mixed-species-biofilms
#12
Jinzhi He, Dongyeop Kim, Xuedong Zhou, Sang-Joon Ahn, Robert A Burne, Vincent P Richards, Hyun Koo
Early childhood caries (ECC), which can lead to rampant tooth-decay that is painful and costly to treat, is one of the most prevalent infectious diseases affecting children worldwide. Previous studies support that interactions between Streptococcus mutans and Candida albicans are associated with the pathogenesis of ECC. The presence of Candida enhances S. mutans growth, fitness and accumulation within biofilms in vitro, although the molecular basis for these behaviors is undefined. Using an established co-cultivation biofilm model and RNA-Seq, we investigated how C...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28642736/identification-of-an-efflux-transporter-lmrb-regulating-stress-response-and-extracellular-polysaccharide-synthesis-in-streptococcus-mutans
#13
Jia Liu, Lihong Guo, Jianwei Liu, Jianying Zhang, Huihui Zeng, Yang Ning, Xi Wei
Efflux transporters have been implicated in regulating bacterial virulence properties such as resistance to antibiotics, biofilm formation and colonization. The pathogenicity of Streptococcus mutans, the primary etiologic agent of human dental caries, relies on the bacterium's ability to form biofilms on tooth surface. However, the studies on efflux transporters in S. mutans are scare and the function of these transporters remained to be clarified. In this study, we identified an efflux transporter (LmrB) in S...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28642545/high-throughput-screening-of-small-molecule-inhibitors-of-the-streptococcus-quorum-sensing-signal-pathway
#14
Seiji Ishii, Kenji Fukui, Satoshi Yokoshima, Kazuo Kumagai, Youko Beniyama, Tetsuya Kodama, Tohru Fukuyama, Takayoshi Okabe, Tetsuo Nagano, Hirotatsu Kojima, Takato Yano
The main components of the quorum-sensing system are expected to be favorable targets for drug development to combat various chronic infectious diseases. ComA of Streptococcus is an ATP-binding cassette transporter containing a peptidase domain (PEP), which is essential for the quorum-sensing signal production. Using high-throughput screening, we found a potent small molecule that suppressed the S. mutans quorum-sensing pathway through inhibition of PEP activity. The compound effectively attenuated the biofilm formation and competence development of S...
June 22, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28642143/biofilm-formation-by-microsporum-canis
#15
L J Danielli, W Lopes, M H Vainstein, A M Fuentefria, M A Apel
No abstract text is available yet for this article.
June 19, 2017: Clinical Microbiology and Infection
https://www.readbyqxmd.com/read/28641671/occurrence-of-aerobic-and-anaerobic-bacteria-in-the-consumer-end-of-a-water-distribution-system
#16
Süleyman Hoca, Miray Üstüntürk-Onan, Esra Ilhan-Sungur
  In this study, mixed species biofilm formation including sulphate-reducing bacteria (SRB) on polypropylene surface and bacteriology of network water were investigated in a model water distribution system during a nine-month period. Water and biofilm samples were analyzed for the enumeration of aerobic heterotrophic bacteria (AHB), anaerobic heterotrophic bacteria (ANHB) and SRB. The number of live/dead bacteria was also analyzed by epifluorescence microscopy. In addition, extracellular polysaccharide substances (EPS) extraction, carbohydrate analysis and scanning electron microscope observation were performed...
July 1, 2017: Water Environment Research: a Research Publication of the Water Environment Federation
https://www.readbyqxmd.com/read/28639672/imaging-biofilm-encased-microorganisms-using-carbon-dots-derived-from-l-plantarum
#17
Fengming Lin, Chengcheng Li, Li Dong, Degang Fu, Zhan Chen
Imaging biofilm-encased microorganisms is challenging due to them being shielded by a sticky and strong extracellular polymeric substance matrix that blocks the penetration of dyes, but is especially important for biofilm study. Here, carbon dots, namely CDs-605, were used for successfully imaging biofilm-encased microorganisms. We demonstrated that such CDs-605 could be easily synthesized from L. plantarum by one-step hydrothermal carbonization. The negatively charged CDs-605 were composed of C, H, O, N, P, and S and contained various functional groups, including -OH, -COOH, and -CONH-...
June 22, 2017: Nanoscale
https://www.readbyqxmd.com/read/28639245/biofilm-forming-ability-and-clonality-in-acinetobacter-baumannii-strains-isolated-from-urine-samples-and-urinary-catheters-in-different-european-hospitals
#18
Claudia Vuotto, Filipa Grosso, Francesca Longo, Maria Pia Balice, Mariana Carvalho de Barros, Luisa Peixe, Gianfranco Donelli
OBJECTIVE: Biofilm formation has been associated with the persistence of Acinetobacter baumannii in hospital settings and its propensity to cause infection. We investigated the adhesion ability and clonality of 128 A. baumannii isolates recovered from urine and urinary catheters of patients admitted to 5 European hospitals during 1991-2013. METHODS: Isolates identification was confirmed by rpoB sequencing and by the presence of blaOXA-51. The presence of carbapenemases was detected by PCR...
June 22, 2017: Advances in Experimental Medicine and Biology
https://www.readbyqxmd.com/read/28639021/evapotranspiration-versus-oxygen-intrusion-which-is-the-main-force-in-alleviating-bioclogging-of-vertical-flow-constructed-wetlands-during-a-resting-operation
#19
Guofen Hua, Qiuwen Chen, Jun Kong, Man Li
Clogging is the most significant challenge limiting the application of constructed wetlands. Application of a forced resting period is a practical way to relieve clogging, particularly bioclogging. To reveal the alleviation mechanisms behind such a resting operation, evapotranspiration and oxygen flux were studied during a resting period in a laboratory vertical-flow constructed wetland model through physical simulation and numerical model analysis. In addition, the optimum theoretical resting duration was determined based on the time required for oxygen to completely fill the pores, i...
June 21, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28638805/increased-zinc-availability-enhances-initial-aggregation-and-biofilm-formation-of-streptococcus-pneumoniae
#20
Lindsey R Brown, Rachel C Caulkins, Tyler E Schartel, Jason W Rosch, Erin S Honsa, Stacey Schultz-Cherry, Victoria A Meliopoulos, Sean Cherry, Justin A Thornton
Bacteria growing within biofilms are protected from antibiotics and the immune system. Within these structures, horizontal transfer of genes encoding virulence factors, and promoting antibiotic resistance occurs, making biofilms an extremely important aspect of pneumococcal colonization and persistence. Identifying environmental cues that contribute to the formation of biofilms is critical to understanding pneumococcal colonization and infection. Iron has been shown to be essential for the formation of pneumococcal biofilms; however, the role of other physiologically important metals such as copper, zinc, and manganese has been largely neglected...
2017: Frontiers in Cellular and Infection Microbiology
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