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Biofilm formation

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https://www.readbyqxmd.com/read/29165147/enterococcus-hirae-biofilm-formation-on-hospital-material-surfaces-and-effect-of-new-biocides
#1
Silvia Di Lodovico, Valentina Cataldi, Emanuela Di Campli, Elisabetta Ancarani, Luigina Cellini, Mara Di Giulio
BACKGROUND: Nowadays, the bacterial contamination in the hospital environment is of particular concern because the hospital-acquired infections (HAIs), also known as nosocomial infections, are responsible for significant morbidity and mortality. This work evaluated the capability of Enterococcus hirae to form biofilm on different surfaces and the action of two biocides on the produced biofilms. METHODS: The biofilm formation of E. hirae ATCC 10541 was studied on polystyrene and stainless steel surfaces through the biomass quantification and the cell viability at 20 and 37 °C...
August 2, 2017: Environmental Health and Preventive Medicine
https://www.readbyqxmd.com/read/29164822/a-transposon-mutant-library-of-bacillus-cereus-atcc-10987-reveals-novel-genes-required-for-biofilm-formation-and-implicates-motility-as-an-important-factor-for-pellicle-biofilm-formation
#2
Mira Okshevsky, Matilde Greve Louw, Elena Otero Lamela, Martin Nilsson, Tim Tolker-Nielsen, Rikke Louise Meyer
Bacillus cereus is one of the most common opportunistic pathogens causing foodborne illness, as well as a common source of contamination in the dairy industry. B. cereus can form robust biofilms on food processing surfaces, resulting in food contamination due to shedding of cells and spores. Despite the medical and industrial relevance of this species, the genetic basis of biofilm formation in B. cereus is not well studied. In order to identify genes required for biofilm formation in this bacterium, we created a library of 5000 +  transposon mutants of the biofilm-forming strain B...
November 22, 2017: MicrobiologyOpen
https://www.readbyqxmd.com/read/29164404/size-selectivity-in-antibiofilm-activity-of-3-diphenylphosphino-propanoic-acid-coated-gold-nanomaterials-against-gram-positive-staphylococcus-aureus-and-streptococcus-mutans
#3
Dania Ahmed, Ayaz Anwar, Anum Khalid Khan, Ayaz Ahmed, Muhammad Raza Shah, Naveed Ahmed Khan
Biofilm formation by pathogenic bacteria is one of the major threats in hospital related infections, hence inhibiting and eradicating biofilms has become a primary target for developing new anti-infection approaches. The present study was aimed to develop novel antibiofilm agents against two Gram-positive bacteria; Staphylococcus aureus (ATCC 43300) and Streptococcus mutans (ATCC 25175) using gold nanomaterials conjugated with 3-(diphenylphosphino)propionic acid (Au-LPa). Gold nanomaterials with different sizes as 2-3 nm small and 9-90 nm (50 nm average size) large were stabilized by LPa via different chemical synthetic strategies...
November 21, 2017: AMB Express
https://www.readbyqxmd.com/read/29164352/assessment-of-methylobacterium-oryzae-cbmb20-aggregates-for-salt-tolerance-and-plant-growth-promoting-characteristics-for-bio-inoculant-development
#4
Mak Chanratana, Gwang Hyun Han, Aritra Roy Choudhury, Seshadri Sundaram, Md Abdul Halim, Ramasamy Krishnamoorthy, Yeongyeong Kang, Tongmin Sa
Salinity is one of the major factors contributing to the loss of crop productivity and thereby impacting livelihood of people in more than 100 countries of the world and the area of land affected by salinity is increasing day by day. This will worsen due to various factors such as drought that might result in high soil salinity. Use of plant growth promoting rhizobacteria is one of the promising eco-friendly strategies for salinity stress management as part of sustainable agricultural practices. However, it requires selecting rhizobacteria with good survivability and adaptation to salt stress...
November 21, 2017: AMB Express
https://www.readbyqxmd.com/read/29164072/a-new-strain-collection-for-improved-expression-of-outer-membrane-proteins
#5
Ina Meuskens, Marcin Michalik, Nandini Chauhan, Dirk Linke, Jack C Leo
Almost all integral membrane proteins found in the outer membranes of Gram-negative bacteria belong to the transmembrane β-barrel family. These proteins are not only important for nutrient uptake and homeostasis, but are also involved in such processes as adhesion, protein secretion, biofilm formation, and virulence. As surface exposed molecules, outer membrane β-barrel proteins are also potential drug and vaccine targets. High production levels of heterologously expressed proteins are desirable for biochemical and especially structural studies, but over-expression and subsequent purification of membrane proteins, including outer membrane proteins, can be challenging...
2017: Frontiers in Cellular and Infection Microbiology
https://www.readbyqxmd.com/read/29163446/more-than-gliding-involvement-of-gldd-and-gldg-in-the-virulence-of-flavobacterium-psychrophilum
#6
David Pérez-Pascual, Tatiana Rochat, Brigitte Kerouault, Esther Gómez, Fabienne Neulat-Ripoll, Celine Henry, Edwige Quillet, Jose A Guijarro, Jean F Bernardet, Eric Duchaud
A fascinating characteristic of most members of the genus Flavobacterium is their ability to move over surfaces by gliding motility. Flavobacterium psychrophilum, an important pathogen of farmed salmonids worldwide, contains in its genome the 19 gld and spr genes shown to be required for gliding or spreading in Flavobacterium johnsoniae; however, their relative role in its lifestyle remains unknown. In order to address this issue, two spreading deficient mutants were produced as part of a Tn4351 mutant library in F...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163441/antifungal-and-anti-biofilm-activity-of-essential-oil-active-components-against-cryptococcus-neoformans-and-cryptococcus-laurentii
#7
Poonam Kumari, Rutusmita Mishra, Neha Arora, Apurva Chatrath, Rashmi Gangwar, Partha Roy, Ramasare Prasad
Cryptococcosis is an emerging and recalcitrant systemic infection occurring in immunocompromised patients. This invasive fungal infection is difficult to treat due to the ability of Cryptococcus neoformans and Cryptococcus laurentii to form biofilms resistant to standard antifungal treatment. The toxicity concern of these drugs has stimulated the search for natural therapeutic alternatives. Essential oil and their active components (EO-ACs) have shown to possess the variety of biological and pharmacological properties...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163440/nutrient-composition-promotes-switching-between-pellicle-and-bottom-biofilm-in-salmonella
#8
Sonia Paytubi, Cintia Cansado, Cristina Madrid, Carlos Balsalobre
Salmonella is one of the most frequently reported causes of foodborne illness worldwide. Non-typhoidal serovars cause gastroenteritis in humans. Salmonella can grow on surfaces forming biofilms, contributing to its persistence since biofilms are difficult to eradicate due to the high resistance to antimicrobials and disinfectants. It has been described that there are two crucial biofilm promoting factors in Salmonella: curli and cellulose. The expression of both factors is coordinately regulated by the transcriptional regulator CsgD...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163415/combination-susceptibility-testing-of-common-antimicrobials-in-vitro-and-the-effects-of-sub-mic-of-antimicrobials-on-staphylococcus-aureus-biofilm-formation
#9
Bing Yang, Zhixin Lei, Yishuang Zhao, Saeed Ahmed, Chunqun Wang, Shishuo Zhang, Shulin Fu, Jiyue Cao, Yinsheng Qiu
The current study was conducted to evaluate the antibacterial combination efficacies, and whether the sub-inhibitory concentrations (sub-MIC) of antibiotics can influent on the biofilm formation of S. aureus. The minimum inhibitory concentration (MIC) of common antibacterial drugs was determined in vitro against clinical isolates of Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), and Pasteurella multocida (P. multocida) alone and in combination with each other by using the broth microdilution method and the checkerboard micro-dilution method analyzed with the fractional inhibitory concentration index (FICI), respectively...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163397/functionality-of-root-associated-bacteria-along-a-salt-marsh-primary-succession
#10
Miao Wang, Erqin Li, Chen Liu, Alexandre Jousset, Joana F Salles
Plant-associated bacteria are known for their high functional trait diversity, from which many are likely to play a role in primary and secondary succession, facilitating plant establishment in suboptimal soils conditions. Here we used an undisturbed salt marsh chronosequence that represents over 100 years of soil development to assess how the functional traits of plant associated bacteria respond to soil type, plant species and plant compartment. We isolated and characterized 808 bacterial colonies from the rhizosphere soil and root endosphere of two salt marsh plants, Limonium vulgare and Artemisia maritima, along the chronosequence...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163396/synergistic-antifungal-effect-of-fluconazole-combined-with-licofelone-against-resistant-candida-albicans
#11
Xinning Liu, Tao Li, Decai Wang, Yilei Yang, Wenwen Sun, Jianqiao Liu, Shujuan Sun
Candida albicans (C. albicans) is one of the important opportunistic fungal pathogens that is closely associated with disseminated or chronic infections. The objective of this study is to evaluate the synergistic antifungal effect of licofelone, which is dual microsomal prostaglandin E2 synthase/lipoxygenase (mPGES-1/LOX) inhibitor in combination with fluconazole against C. albicans. Here our results showed that licofelone (16 μg/mL) can synergistically work with fluconazole (1 μg/mL) against planktonic cells of fluconazole-resistant C...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163392/clonal-expansion-of-biofilm-forming-salmonella-typhimurium-st34-with-multidrug-resistance-phenotype-in-the-southern-coastal-region-of-china
#12
Wanli Li, Yinghui Li, Yao Liu, Xiaolu Shi, Min Jiang, Yiman Lin, Yaqun Qiu, Qian Zhang, Qiongcheng Chen, Li Zhou, Qun Sun, Qinghua Hu
To disclose the antibiotics susceptibility and wide adaptability of commonly occurring genotypes of Salmonella Typhimurium, the antibiotic resistance and biofilm formation of different multi-locus sequence typing (MLST) types of a collection of 240 S. Typhimurium isolates (33 food and 207 clinical ones) during 2010-2014 in Shenzhen were analyzed. Among these strains, 167 was ST34 (69.58%), and 57 was ST19 (23.75%), respectively. A total of 159 (95.21%) ST34 strains displayed the multidrug resistant phenotype (≥ three classes of antibiotic), whereas only 23 (40...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163384/bacillus-subtilis-biofilm-development-a-computerized-study-of-morphology-and-kinetics
#13
Sarah Gingichashvili, Danielle Duanis-Assaf, Moshe Shemesh, John D B Featherstone, Osnat Feuerstein, Doron Steinberg
Biofilm is commonly defined as accumulation of microbes, embedded in a self-secreted extra-cellular matrix, on solid surfaces or liquid interfaces. In this study, we analyze several aspects of Bacillus subtilis biofilm formation using tools from the field of image processing. Specifically, we characterize the growth kinetics and morphological features of B. subtilis colony type biofilm formation and compare these in colonies grown on two different types of solid media. Additionally, we propose a model for assessing B...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163381/functional-characterization-of-acinetobacter-baumannii-lacking-the-rna-chaperone-hfq
#14
Han-Yueh Kuo, Hsuan-Hao Chao, Po-Cheng Liao, Long Hsu, Kai-Chih Chang, Chi-Hua Tung, Chang-Hua Chen, Ming-Li Liou
The RNA chaperone Hfq is involved in the riboregulation of diverse genes via small RNAs. Recent studies have demonstrated that Hfq contributes to the stress response and the virulence of several pathogens, and the roles of Hfq vary among bacterial species. Here, we attempted to elucidate the role of Hfq in Acinetobacter baumannii ATCC 17978. In the absence of hfq, A. baumannii exhibited retarded cell growth and was highly sensitive to environmental stress, including osmotic and oxidative pressure, pH, and temperature...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163377/wild-grape-associated-yeasts-as-promising-biocontrol-agents-against-vitis-vinifera-fungal-pathogens
#15
Gustavo Cordero-Bueso, Nicola Mangieri, David Maghradze, Roberto Foschino, Federica Valdetara, Jesús M Cantoral, Ileana Vigentini
The increasing level of hazardous residues in the environment and food chains has led the European Union to restrict the use of chemical fungicides. Thus, exploiting new natural antagonistic microorganisms against fungal diseases could serve the agricultural production to reduce pre- and post-harvest losses, to boost safer practices for workers and to protect the consumers' health. The main aim of this work was to evaluate the antagonistic potential of epiphytic yeasts against Botrytis cinerea, Aspergillus carbonarius, and Penicillium expansum pathogen species...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29163197/variation-in-enamel-formation-genes-influences-enamel-demineralization-in-vitro-in-a-streptococcus-mutans-biofilm-model
#16
Liangyue Pang, Qinghui Zhi, Peilin Zhuang, Lixia Yu, Ye Tao, Huancai Lin
Genetic studies have shown that variations in enamel formation genes are associated with caries susceptibility. The aim of this study was to test in vitro whether variants in these genes are associated with dental enamel demineralization in a Streptococcus mutans biofilm model. DNA and enamel samples were obtained from 213 individuals. DNA was extracted from saliva, and 16 single nucleotide polymorphisms were analyzed. The physical and chemical properties of sound enamel samples and the mineral loss and the lesion depth of the demineralized enamel samples under cariogenic challenge were analyzed...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29162873/rapid-in-situ-imaging-and-whole-genome-sequencing-of-biofilm-in-neonatal-feeding-tubes-a-clinical-proof-of-concept
#17
Pauline Ogrodzki, Chi Shing Cheung, Mohamed Saad, Khaled Dahmani, Rebecca Coxill, Haida Liang, Stephen J Forsythe
The bacterial flora of nasogastric feeding tubes and faecal samples were analysed for a low-birth weight (725 g) neonate EGA 25 weeks in intensive care. Samples were collected at age 6 and 8 weeks of life. Optical coherence tomography (OCT) was used to visualise bacterial biofilms inside the nasogastric feeding tubes. The biofilm was heterogeneously distributed along the tube lumen wall, and had a depth of up to 500 µm. The bacterial biofilm and faecal samples included Enterococcus faecalis and Enterobacter hormaechei...
November 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29162785/relevance-of-surface-characteristics-in-the-adhesiveness-of-polymicrobial-biofilms-to-crown-restoration-materials
#18
Ayako Teranaka, Kiyoshi Tomiyama, Katsura Ohashi, Kaori Miyake, Tota Shimizu, Nobushiro Hamada, Yoshiharu Mukai, Satoshi Hirayama, Tomotaro Nihei
We used a polymicrobial (PM) biofilm model to examine associations of bacterial adhesiveness with surface characteristics of various dental materials. Four types of dental materials (apatite pellet, zirconia, ceramic, and composite resin) with rough and mirror surfaces were used. Surface roughness, surface free energy, zeta potential, and colony-forming units (CFUs) of the biofilm formations were measured. Biofilms were cultured for 24 h under anaerobic conditions, plated onto blood agar medium, and anaerobically cultured for 4 days...
November 22, 2017: Journal of Oral Science
https://www.readbyqxmd.com/read/29162713/capsular-polysaccharide-interferes-with-biofilm-formation-by-pasteurella-multocida-serogroup-a
#19
Briana Petruzzi, Robert E Briggs, W Edward Swords, Cristina De Castro, Antonio Molinaro, Thomas J Inzana
Pasteurella multocida is an important multihost animal and zoonotic pathogen that is capable of causing respiratory and multisystemic diseases, bacteremia, and bite wound infections. The glycosaminoglycan capsule of P. multocida is an essential virulence factor that protects the bacterium from host defenses. However, chronic infections (such as swine atrophic rhinitis and the carrier state in birds and other animals) may be associated with biofilm formation, which has not been characterized in P. multocida Biofilm formation by clinical isolates was inversely related to capsule production and was confirmed with capsule-deficient mutants of highly encapsulated strains...
November 21, 2017: MBio
https://www.readbyqxmd.com/read/29162075/the-antibiofilm-efficacy-of-nitric-oxide-on-soft-contact-lenses
#20
Dong Ju Kim, Joo-Hee Park, Marth Kim, Choul Yong Park
BACKGROUND: To investigate the antibiofilm efficacy of nitric oxide (NO) on soft contact lenses. METHODS: Nitrite (NO precursor) release from various concentrations (0-1000 μM) of sodium nitrite (NaNO2, NO donor) was measured by Griess Assay. Cell viability assay was performed using human corneal epithelial cell under various concentration (0-1000 μM) of NaNO2. Biofilm formation on soft contact lenses was achieved by adding Staphylococcus aureus or Pseudomonas aeruginosa to the culture media...
November 21, 2017: BMC Ophthalmology
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