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Oocyte retrieval

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https://www.readbyqxmd.com/read/30516453/does-the-meiotic-spindle-really-predicts-embryo-implantation-and-live-birth-rates-an-update
#1
Sabela García-Oro, Diana Valverde
SummaryThe aim of this study was to determine the capacity of the meiotic spindle (MS) to predict embryo implantation and live birth rates. For this purpose, we performed a broad systematic literature search. Of all publications retrieved, only those in which the implantation rates were related to some characteristics of the MS were evaluated. Despite the different methodology used in all the chosen studies, presence of the MS in oocytes was found to be positively associated with embryo implantation. Moreover, high retardance values, as well as strict criteria of normality in the MS structure, are significantly related to higher embryo implantation numbers and live birth rates...
December 5, 2018: Zygote: the Biology of Gametes and Early Embryos
https://www.readbyqxmd.com/read/30515934/effect-of-dna-damage-of-cumulus-oophorus-cells-and-lymphocytes-analyzed-by-alkaline-comet-assay-on-oocyte-quality-and-intracytoplasmic-sperm-injection-success-among-patients-with-polycystic-ovary-syndrome
#2
Esra N Tola, Pınar A Koşar, Dilek U Karatopuk, Okan Sancer, Baha Oral
AIM: Cumulus oophorus cells (COC) exhibit a close relationship with the oocytes. We aimed to determine the role of the DNA integrity of COC and lymphocytes on the oocyte and embryo quality and intracytoplasmic sperm injection (ICSI) success. METHODS: The COC obtained with the mechanical denudation of each oocyte and the lymphocytes obtained on oocyte retrieval day from 69 infertile polycystic ovary syndrome (PCOS) patients were used. The tail length, tail moment and tail DNA percentage were evaluated using an alkaline comet assay...
December 4, 2018: Journal of Obstetrics and Gynaecology Research
https://www.readbyqxmd.com/read/30503199/follicular-fluid-humanin-concentration-is-related-to-ovarian-reserve-markers-and-clinical-pregnancy-after-ivf-icsi-a-pilot-study
#3
Meng Rao, Fang Zhou, Li Tang, Zhengyan Zeng, Shifu Hu, Yingying Wang, Dandan Ke, Guiping Cheng, Wei Xia, Ling Zhang, Changhong Zhu
RESEARCH QUESTION: Is humanin present in the human ovary and follicular fluid? What relationship exists between humanin concentration in the follicular fluid and ovarian reserve and clinical outcomes after IVF and intracytoplasmic sperm injection (ICSI)? DESIGN: Follicular fluid samples were collected from 179 patients undergoing their first IVF or ICSI cycle during oocyte retrieval. Ovarian tissues were collected from two patients undergoing surgery for ovarian cysts...
November 23, 2018: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/30503134/the-impact-of-malignancy-on-response-to-ovarian-stimulation-for-fertility-preservation-a-meta-analysis
#4
Volkan Turan, Molly M Quinn, Nurten Dayioglu, Mitchell P Rosen, Kutluk Oktay
OBJECTIVE: To evaluate the impact of cancer diagnosis on response to ovarian stimulation for fertility preservation. DESIGN: Meta-analysis. SETTING: Not applicable. PATIENT(S): An electronic-based search was performed with the use of PubMed until May 2018 limited to English-language articles. In the final analysis, 10 case-controlled retrospective cohort studies were included, comparing ovarian response to stimulation between women with cancer and age-matched healthy women (control group)...
December 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/30501453/endometrial-but-not-ovarian-response-is-associated-with-clinical-outcomes-and-can-be-improved-by-prolonged-pituitary-downregulation-in-patients-with-thin-and-medium-endometrium
#5
Jianyuan Song, Xuejiao Sun, Kun Qian
The aim of this study is to investigate the effect of ovarian and endometrial response on live birth rates (LBRs) in young normal and high responders and prolonged pituitary downregulation on endometrial receptivity and clinical outcomes in patients with different endometrial thickness. Between January 2013 and December 2017, 9511 patients underwent first in vitro fertilization (IVF)/intracytoplasmic sperm injection (ICSI) cycles with age ≤35 years, follicle stimulating hormone < 10 IU/l, and ≥4 retrieved oocytes were conducted...
December 3, 2018: Reproductive Sciences
https://www.readbyqxmd.com/read/30500907/to-test-or-not-to-test-a-framework-for-counselling-patients-on-preimplantation-genetic-testing-for-aneuploidy-pgt-a
#6
Lauren A Murphy, Emily A Seidler, Denis A Vaughan, Nina Resetkova, Alan S Penzias, Thomas L Toth, Kim L Thornton, Denny Sakkas
STUDY QUESTION: What is the treatment path and cumulative live birth (CLB) rate from a single oocyte retrieval of patients who intend to pursue PGT-A at the start of an IVF cycle compared to matched controls? SUMMARY ANSWER: The choice of PGT-A at the start of the first IVF cycle decreases the CLB per oocyte retrieval for patients <38 years of age, however patients ≥38 years of age benefit significantly per embryo transfer (ET) when live birth (LB) is evaluated...
November 30, 2018: Human Reproduction
https://www.readbyqxmd.com/read/30498475/atf4-contributes-to-ovulation-via-regulating-cox2-pge2-expression-a-potential-role-of-atf4-in-pcos
#7
Fangfang Di, Jiansheng Liu, Shang Li, Guangxin Yao, Yan Hong, Zi-Jiang Chen, Weiping Li, Yanzhi Du
Ovulatory disorder is common in patients with hyperprolactinemia or polycystic ovary syndrome (PCOS). Previous studies have shown that ATF4 plays critical role in apoptosis and glucose homeostasis, but its role in regulating reproductive function was not explored. The present study investigated the role of ATF4 in ovarian ovulatory function. Human granulosa cells (hGCs) from 48 women newly diagnosed with PCOS and 37 controls were used to determine ATF4 expression. In vitro cultured hGCs were used to detect the upstream and downstream genes of ATF4...
2018: Frontiers in Endocrinology
https://www.readbyqxmd.com/read/30486636/cumulative-live-birth-rates-in-ivf
#8
Panagiotis Drakopoulos, Joaquín Errázuriz, Samuel Santos-Ribeiro, Herman Tournaye, Alberto Vaiarelli, Nicola Pluchino, Christophe Blockeel, Nikolaos Polyzos
INTRODUCTION: Traditionally, in vitro fertilization (IVF) success rates have been reported in terms of live birth per fresh cycle or embryo transfer. However, reporting IVF outcomes using cumulative live birth rates (LBR), defined as the first live birth following the use of all fresh and frozen embryos derived from a single ovarian stimulation cycle appears to be a better measure of IVF treatment success. EVIDENCE ACQUISITION AND SYNTHESIS: Although there is an initial increase in fresh LBR with the number of oocytes retrieved, LBR either reach a plateau or may even decline when more than 15-20 oocytes are harvested...
November 27, 2018: Minerva Ginecologica
https://www.readbyqxmd.com/read/30485412/changes-in-the-incidence-and-uptake-of-reproductive-surgery-versus-in-vitro-fertilisation-in-australia-between-2001-and-2015-a-population-based-study
#9
Leon Foster, Stephen J Robson, Anusch Yazdani, Pauline Y O'Shaughnessy
OBJECTIVE: Improvements in success rates of assisted reproduction led to predictions that infertility surgery in both women and men would become extinct in developed countries. We sought to identify the changes in reproductive surgery that occurred between 2001 and 2015 to determine whether these predictions have been accurate. DESIGN: The Australian Institute of Health and Welfare (AIHW) national procedural dataset and the Australian Medicare Benefits Scheme (MBS) claims database were searched for procedure data for male and female reproductive surgery and assisted reproduction from January 2001 to December 2015...
November 28, 2018: Australian & New Zealand Journal of Obstetrics & Gynaecology
https://www.readbyqxmd.com/read/30481674/urinary-concentrations-of-phenols-and-phthalate-metabolites-reflect-extracellular-vesicle-microrna-expression-in-follicular-fluid
#10
Rosie M Martinez, Russ Hauser, Liming Liang, Abdallah Mansur, Michal Adir, Laura Dioni, Catherine Racowsky, Valentina Bollati, Andrea A Baccarelli, Ronit Machtinger
BACKGROUND: Phenols and phthalates are potential endocrine disrupting chemicals (EDCs) that are associated with adverse health outcomes. These EDCs dysregulate a number of biomolecules and pathways, including microRNAs. MicroRNAs can be carried in transport systems called extracellular vesicles (EVs) that are present in most biofluids. EVs in the follicular fluid, which fills the ovarian follicle and influences oocyte developmental competency, carry microRNAs (EV-miRNAs) that have been associated with In Vitro Fertilization (IVF) outcomes...
November 24, 2018: Environment International
https://www.readbyqxmd.com/read/30480769/in-vitro-maturation-in-subfertile-women-with-polycystic-ovarian-syndrome-undergoing-assisted-reproduction
#11
REVIEW
Charalampos S Siristatidis, Abha Maheshwari, Dennis Vaidakis, Siladitya Bhattacharya
BACKGROUND: Polycystic ovarian syndrome (PCOS) occurs in 4% to 7% of all women of reproductive age and 50% of women presenting with subfertility. Subfertility affects 15% to 20% of couples trying to conceive. A significant proportion of these women ultimately need assisted reproductive technology (ART). In vitro fertilisation (IVF) is one of the assisted reproduction techniques employed to raise the chances of achieving a pregnancy. For the standard IVF technique, stimulating follicle development and growth before oocyte retrieval is essential, for which a large number of different methods combining gonadotrophins with a gonadotrophin-releasing hormone (GnRH) agonist or antagonist are used...
November 15, 2018: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/30478807/fertility-preservation-in-women-with-malignancies-the-accuracy-of-antral-follicle-count-collected-randomly-during-the-menstrual-cycle-in-predicting-the-number-of-oocytes-retrieved
#12
Francesca Filippi, Fabio Martinelli, Alessio Paffoni, Marco Reschini, Francesco Raspagliesi, Edgardo Somigliana
PURPOSE: To evaluate the capacity of random antral follicle count (AFC), i.e., AFC recorded at any time during the menstrual cycle, to predict the number of retrieved mature oocytes in women with malignancies undergoing random start ovarian hyperstimulation METHODS: A consecutive series of 72 women with malignancies who underwent ovarian hyperstimulation aimed at egg freezing between July 2014 and December 2016 was retrospectively reviewed. A standardized random start protocol was used for all women...
November 26, 2018: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/30477915/autologous-mitochondrial-transfer-as-a-complementary-technique-to-intracytoplasmic-sperm-injection-to-improve-embryo-quality-in-patients-undergoing-in-vitro-fertilization-a-randomized-pilot-study
#13
Elena Labarta, Maria José de Los Santos, Sonia Herraiz, Maria José Escribá, Alicia Marzal, Anna Buigues, Antonio Pellicer
OBJECTIVE: To study if autologous mitochondrial transfer (AUGMENT) improves outcome in patients with previously failed in vitro fertilization (IVF). DESIGN: Randomized, controlled, triple-blind, experimental study. SETTING: Private infertility center, Valencian Institute of Infertility (IVI-RMA), Valencia, Spain. PATIENT(S): Infertile women ≤42 years of age, body mass index <30 kg/m2 , antimüllerian hormone ≥4 pmol/L, >5 million/mL motile sperm, at least one previous IVF with at least five metaphase oocytes (MIIs) collected, and low embryo quality...
November 24, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/30477755/how-time-to-healthy-singleton-delivery-could-affect-decision-making-during-infertility-treatment-a-delphi-consensus
#14
Ernesto Bosch, Carlo Bulletti, Alan B Copperman, Renato Fanchin, Hakan Yarali, Carlos A Petta, Nikolaos P Polyzos, Daniel Shapiro, Filippo Maria Ubaldi, Juan A Garcia Velasco, Salvatore Longobardi, Thomas D'Hooghe, Peter Humaidan
RESEARCH QUESTION: How might time to healthy singleton delivery affect decision-making during infertility treatment? DESIGN: This was a Delphi consensus investigating expert opinion that comprised three steps. In Step 1, 12 experts developed statements. In Step 2, 27 experts (including 12 from Step 1) voted (online survey) on their agreement/disagreement with each statement (providing reasons). Consensus was reached if ≥66% of participants agreed/disagreed. Statements not reaching consensus were revised and the process repeated until consensus was achieved...
November 7, 2018: Reproductive Biomedicine Online
https://www.readbyqxmd.com/read/30476100/is-more-better-a-higher-oocyte-yield-is-independently-associated-with-more-day-3-euploid-embryos-after-icsi
#15
Christos A Venetis, Liza Tilia, Erin Panlilio, Andrew Kan
STUDY QUESTION: Is the number of oocytes retrieved after ovarian stimulation for ICSI independently associated with the number of day-3 euploid embryos (EE)? SUMMARY ANSWER: A larger oocyte yield is independently associated with more day-3 EE, although the expected benefit decreases significantly with advancing age. WHAT IS KNOWN ALREADY: Although traditionally ovarian stimulation aims at collecting more than one oocyte in order to increase the chance of pregnancy, there is evidence suggesting that excessive ovarian response leads to lower live birth rates...
November 24, 2018: Human Reproduction
https://www.readbyqxmd.com/read/30475413/cervico-vaginal-secretion-cytokine-profile-a-non-invasive-approach-to-study-the-endometrial-receptivity-in-ivf-cycles
#16
Nunzia Zanotta, Lorenzo Monasta, Kristina Skerk, Stefania Luppi, Monica Martinelli, Giuseppe Ricci, Manola Comar
PROBLEM: Cytokines have a significant role in the process of embryo implantation, trophoblast growth, and differentiation by modulating the immune and endocrine system. The aim of this study was to investigate the profile of a large set of cytokines in the cervico-vaginal washing of women undergoing IVF, to explore the association of these proteins with a good receptive endometrium. METHOD OF STUDY: A cohort of 155 women scheduled for IVF cycle was recruited. All patients were asymptomatic for genitourinary infections and had been screened for chlamydia, mycoplasma, and other bacterial infections...
November 26, 2018: American Journal of Reproductive Immunology: AJRI
https://www.readbyqxmd.com/read/30473208/towards-complication-free-assisted-reproduction-technology
#17
REVIEW
Samuel Santos-Ribeiro, Shari Mackens, Annalisa Racca, Christophe Blockeel
Assisted reproductive technology (ART) has vastly improved over the last 40 years, from a frequently unsuccessful and complicated procedure requiring hospital admission and routine laparoscopy to a fairly simple outpatient technique with relatively high success rates. However, it is important to stress that ART is not without risk and medical complications may still occur. The incidence of most of these ART-related complications is associated with how women undergo ovarian stimulation. For this reason, physicians should be aware that a carefully thought-out ovarian stimulation protocol and cycle monitoring are of paramount importance to maximise the success of the treatment while avoiding potentially life-threating complications to occur in this frequently otherwise healthy patient population...
November 3, 2018: Best Practice & Research. Clinical Endocrinology & Metabolism
https://www.readbyqxmd.com/read/30463623/is-the-aging-human-ovary-still-ticking-expression-of-clock-genes-in-luteinized-granulosa-cells-of-young-and-older-women
#18
Amnon Brzezinski, A Saada, H Miller, N A Brzezinski-Sinai, A Ben-Meir
BACKGROUND: It has been shown - mostly in animal models - that circadian clock genes are expressed in granulosa cells and in corpora luteum and might be essential for the ovulatory process and steroidogenesis. OBJECTIVE: We sought to investigate which circadian clock genes exist in human granulosa cells and whether their expression and activity decrease during aging of the ovary. STUDY DESIGN: Human luteinized granulosa cells were isolated from young (age 18-33) and older (age 39-45) patients who underwent in-vitro fertilization treatment...
November 21, 2018: Journal of Ovarian Research
https://www.readbyqxmd.com/read/30463583/decrease-in-preovulatory-serum-estradiol-is-a-valuable-marker-for-predicting-premature-ovulation-in-natural-unstimulated-in-vitro-fertilization-cycle
#19
Xuefeng Lu, Shuzin Khor, Qianqian Zhu, Lihua Sun, Yun Wang, Qiuju Chen, Ling Wu, Yonglun Fu, Hui Tian, Qifeng Lyu, Renfei Cai, Yanping Kuang
BACKGROUND: Premature ovulation occurs at a high rate in natural-cycle in vitro fertilization (IVF), and cycle cancellation further hampers the overall efficiency of the procedure. While lower levels of estradiol (E2) are observed in preovulatory follicles, it is unclear whether declines in E2 can be used as an effective marker of premature ovulation. METHODS: This retrospective analysis includes 801 natural/unstimulated IVF/ICSI cycles undergoing scheduled ovum pick-up (OPU) and 153 natural/unstimulated IVF/ICSI cycles undergoing emergency OPU at a university IVF center from May 2014 to February 2017...
November 21, 2018: Journal of Ovarian Research
https://www.readbyqxmd.com/read/30460614/accuracy-and-efficacy-of-embryo-transfer-based-on-the-previous-measurement-of-cervical-length-and-total-uterine-length
#20
Panagiotis Bakas, Mara Simopoulou, Maria Giner, Despina Tzanakaki, Eythimios Deligeoroglou
PURPOSE: Aim of the study was to examine the accuracy of embryo transfer based on the previous measurement of cervical length and total uterine length. METHODS: The study has taken place in an academic-assisted reproduction clinic. 248 patients with infertility requiring IVF/ICSI treatment have been included. All patients underwent IVF/ICSI cycles with antagonist protocol and were treated with controlled ovarian stimulation using recombinant FSH (100-400 IU/day)...
November 21, 2018: Archives of Gynecology and Obstetrics
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