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https://www.readbyqxmd.com/read/30506978/the-ddx5-dbp2-subfamily-of-dead-box-rna-helicases
#1
REVIEW
Zheng Xing, Wai Kit Ma, Elizabeth J Tran
The mammalian DEAD-box RNA helicase DDX5, its paralog DDX17, and their orthologs in Saccharomyces cerevisiae and Drosophila melanogaster, namely Dbp2 and Rm62, define a subfamily of DEAD-box proteins. Members from this subfamily share highly conserved protein sequences and cellular functions. They are involved in multiple steps of RNA metabolism including mRNA processing, microRNA processing, ribosome biogenesis, RNA decay, and regulation of long noncoding RNA activities. The DDX5/Dbp2 subfamily is also implicated in transcription regulation, cellular signaling pathways, and energy metabolism...
December 2, 2018: Wiley Interdisciplinary Reviews. RNA
https://www.readbyqxmd.com/read/30501072/development-of-spermatogenesis-in-vitro-in-three-dimensional-culture-from-spermatogonial-cells-of-busulfan-treated-immature-mice
#2
Ali AbuMadighem, Ronnie Solomon, Alina Stepanovsky, Joseph Kapelushnik, QingHua Shi, Eckart Meese, Eitan Lunenfeld, Mahmoud Huleihel
Aggressive chemotherapy may lead to permanent male infertility. Prepubertal males do not generate sperm, but their testes do contain spermatogonial cells (SPGCs) that could be used for fertility preservation. In the present study, we examined the effect of busulfan (BU) on the SPGCs of immature mice, and the possible induction of the survivor SPGCs to develop spermatogenesis in 3D in-vitro culture. Immature mice were injected with BU, and after 0.5⁻12 weeks, their testes were weighed and evaluated histologically compared to the control mice...
November 29, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/30486488/overexpression-of-the-dead-box-rna-helicase-gene-atrh17-confers-tolerance-to-salt-stress-in-arabidopsis
#3
Linh Vu Nguyen, Hye-Yeon Seok, Dong-Hyuk Woo, Sun-Young Lee, Yong-Hwan Moon
Plants adapt to abiotic stresses by complex mechanisms involving various stress-responsive genes. Here, we identified a DEAD-box RNA helicase (RH) gene, AtRH17 , in Arabidopsis , involved in salt-stress responses using activation tagging, a useful technique for isolating novel stress-responsive genes. AT895, an activation tagging line, was more tolerant than wild type (WT) under NaCl treatment during germination and seedling development, and AtRH17 was activated in AT895. AtRH17 possesses nine well-conserved motifs of DEAD-box RHs, consisting of motifs Q, I, Ia, Ib, and II-VI...
November 28, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/30484950/o-glcnacylation-promotes-colorectal-cancer-progression-by-regulating-protein-stability-and-potential-catcinogenic-function-of-ddx5
#4
Nan Wu, Mingzuo Jiang, Yuying Han, Haiming Liu, Yi Chu, Hao Liu, Jiayi Cao, Qiuqiu Hou, Yu Zhao, Bing Xu, Xin Xie
The RNA helicase p68 (DDX5), a key player in RNA metabolism, belongs to the DEAD box family and is involved in the development of colorectal cancer. Here, we found both DDX5 and O-GlcNAcylation are up-regulated in colorectal cancer. In addition, DDX5 protein level is significantly positively correlated with the expression of O-GlcNAcylation. Although it was known DDX5 protein could be regulated by post-translational modification (PTM), how O-GlcNAcylation modification regulated of DDX5 remains unclear. Here we show that DDX5 interacts directly with OGT in the SW480 cell line, which is the only known enzyme that catalyses O-GlcNAcylation in humans...
November 28, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/30483885/a-helicase-links-upstream-orfs-and-rna-structure
#5
REVIEW
Eckhard Jankowsky, Ulf-Peter Guenther
Upstream open reading frames (uORFs) in 5' UTRs of eukaryotic mRNAs are increasingly recognized as important elements that regulate cellular protein synthesis. Since uORFs can start from non-AUG codons, an enormous number of potential uORF initiation sites exists in 5'UTRs. However, only a subset of these sites is used and it has been unclear how actual start sites are selected. Studies of the DEAD-box helicase Ded1p from S. cerevisiae show that translation of uORFs with non-AUG initiation codons occurs upstream of mRNA structures that emerge with defective Ded1p...
November 27, 2018: Current Genetics
https://www.readbyqxmd.com/read/30475900/the-rna-helicase-ddx3x-is-an-essential-mediator-of-innate-antimicrobial-immunity
#6
Daniel Szappanos, Roland Tschismarov, Thomas Perlot, Sandra Westermayer, Katrin Fischer, Ekaterini Platanitis, Fabian Kallinger, Maria Novatchkova, Caroline Lassnig, Mathias Müller, Veronika Sexl, Keiryn L Bennett, Michelle Foong-Sobis, Josef M Penninger, Thomas Decker
DExD/H box RNA helicases, such as the RIG-I-like receptors (RLR), are important components of the innate immune system. Here we demonstrate a pivotal and sex-specific role for the heterosomal isoforms of the DEAD box RNA helicase DDX3 in the immune system. Mice lacking DDX3X during hematopoiesis showed an altered leukocyte composition in bone marrow and spleen and a striking inability to combat infection with Listeria monocytogenes. Alterations in innate immune responses resulted from decreased effector cell availability and function as well as a sex-dependent impairment of cytokine synthesis...
November 26, 2018: PLoS Pathogens
https://www.readbyqxmd.com/read/30459231/interactome-mapping-defines-brg1-a-component-of-the-swi-snf-chromatin-remodeling-complex-as-a-new-partner-of-the-transcriptional-regulator-ctcf
#7
Maria Michela Marino, Camilla Rega, Rosita Russo, Mariangela Valletta, Maria Teresa Gentile, Sabrina Esposito, Ilaria Baglivo, Italia De Feis, Claudia Angelini, Tioajiang Xiao, Gary Felsenfeld, Angela Chambery, Paolo Vincenzo Pedone
The highly conserved zinc finger CCCTC-binding factor (CTCF) regulates genomic imprinting and gene expression by acting as a transcriptional activator or repressor of promoters and insulator of enhancers. The multiple functions of CTCF are accomplished by co-association with other protein partners and are dependent on genomic context and tissue specificity. Despite the critical role of CTCF in the organization of genome structure, to date, only a subset of CTCF interaction partners have been identified. Here we present a large-scale identification of CTCF binding partners using affinity purification and high-resolution LC-MS/MS analysis...
November 20, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/30429220/mapk-and-glycogen-synthase-kinase-3-mediated-phosphorylation-regulates-the-dead-box-protein-modulator-gle1-for-control-of-stress-granule-dynamics
#8
Aditi Aditi, Aaron C Mason, Manisha Sharma, T Renee Dawson, Susan R Wente
Rapid expression of critical stress response factors is a key survival strategy for diseased or stressed cells. During cell stress, translation is inhibited and a pre-existing pool of cytoplasmic mRNA-protein complexes reversibly assembles into cytoplasmic stress granules (SGs). Gle1 is a conserved modulator of RNA-dependent DEAD-box proteins required for mRNA export, translation, and stress responses. Proper Gle1 function is critical, as reflected by some human diseases such as developmental and neurodegenerative disorders and some cancers linked to gle1 mutations...
November 14, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/30419318/roles-of-ddx5-in-the-tumorigenesis-proliferation-differentiation-metastasis-and-pathway-regulation-of-human-malignancies
#9
REVIEW
Nyamao M Rose, Jing Wu, Li Yu, Xiao Xiao, Feng-Min Zhang
The DEAD-box RNA helicase DDX5 is a member of a family of highly conserved proteins involved in gene-expression regulation and ATP-dependent RNA helicase activities. Recently, it has been reported to be aberrantly expressed in many tumors, and is linked to the regulation of many cancer-related pathways. It co-activates many transcription factors, with profound implications for cancer development, and the de-regulation of its functions is ultimately associated with tumor formation and progression. Moreover, it is strongly implicated in the tumorigenesis, invasiveness and metastasis, as well as the proliferation of several cancer types...
November 9, 2018: Biochimica et biophysica acta. Reviews on cancer
https://www.readbyqxmd.com/read/30417346/the-role-of-dead-box-rna-helicase-p68-ddx5-in-the-development-and-treatment-of-breast-cancer
#10
REVIEW
Vida Hashemi, Ali Masjedi, Bita Hazhir-Karzar, Asghar Tanomand, Siamak Sandoghchian Shotorbani, Mohammad Hojjat-Farsangi, Ghasem Ghalamfarsa, Gholamreza Azizi, Enayat Anvari, Behzad Baradaran, Farhad Jadidi-Niaragh
RNA helicase p68 or DEAD (Asp-Glu-Ala-Asp) box polypeptide 5 (DDX5) is a unique member of the highly conserved protein family, which is involved in a broad spectrum of biological processes, including transcription, translation, precursor messenger RNA processing or alternative splicing, and microRNA (miRNA) processing. It has been shown that p68 is necessary for cell growth and participates in the early development and maturation of some organs. Interestingly, p68 is a transcriptional coactivator of numerous oncogenic transcription factors, including nuclear factor-κβ (NF-κβ), estrogen receptor α (ERα), β-catenin, androgen receptor, Notch transcriptional activation complex, p53 and signal transducer, and activator of transcription 3 (STAT3)...
November 11, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/30416857/abnormal-expression-of-yeats4-associates-with-poor-prognosis-and-promotes-cell-proliferation-of-hepatic-carcinoma-cell-by-regulation-the-tcea1-ddx3-axis
#11
Song You, Fuqiang Wang, Qing Hu, Pengtao Li, Changmao Zhang, Yaqi Yu, Yi Zhang, Qiu Li, Qing Bao, Pingguo Liu, Jie Li
YEATS domain containing 4 (YEATS4) is usually amplified and functions as an oncogene in several malignancies, such as colorectum, ovarian, breast and lung. However, the biological role of YEATS4 in hepatocellular carcinoma (HCC) has not yet been discussed. Herein, we found that YEATS4 was significantly upregulated in HCC compared to para-cancerous tissues, and was associated with poor prognosis, large tumor size, poor differentiation and distant metastasis. In addition, YEATS4 promoted HCC cell proliferation and colony formation by binding to and increasing the transcriptional activity of the TCEA1 promoter...
2018: American Journal of Cancer Research
https://www.readbyqxmd.com/read/30401066/validation-of-an-autoclave-procedure-for-sterilization-of-mouse-mus-musculus-carcasses
#12
Marina C Pils, Katrin Kränzler, Petra Beyer, Ulrike Heise, Bastian Pasche, Hermann Riedesel
The sterilization of potentially infectious animal carcasses is an important biologic safety issue in animal facilities operatingas infection or quarantine barriers. However, the literature lacks a validated protocol. Here we describe the validation of anautoclave program suitable for daily use in a small rodent biocontainment unit. We evaluated several procedures for processing mouse carcasses in a standard autoclave. Heat sensors and biologic indicators were implanted inside the peritoneal cavity of dead mice, which were loaded at various densities into IVC cages or metal boxes...
November 6, 2018: Journal of the American Association for Laboratory Animal Science: JAALAS
https://www.readbyqxmd.com/read/30387775/crystal-structures-of-the-n-terminal-domain-of-the-staphylococcus-aureus-dead-box-rna-helicase-csha-and-its-complex-with-amp
#13
Xiaobao Chen, Chengliang Wang, Xuan Zhang, Tian Tian, Jianye Zang
CshA is a DEAD-box RNA helicase that belongs to the DExD/H-box family of proteins, which generally have an RNA-dependent ATPase activity. In Staphylococcus aureus, CshA was identified as a component of the RNA degradosome and plays important roles in RNA turnover. In this study, the crystal structures of the N-terminal RecA-like domain 1 of S. aureus CshA (SaCshAR1 ) and of its complex with AMP (SaCshAR1 -AMP) are reported at resolutions of 1.5 and 1.8 Å, respectively. SaCshAR1 adopts a conserved α/β RecA-like structure with seven parallel strands surrounded by nine α-helices...
November 1, 2018: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/30382588/oct4-sox2-specific-cytotoxic-t-lymphocytes-plus-programmed-cell-death-protein-1-inhibitor-presented-with-synergistic-effect-on-killing-lung-cancer-stem-like-cells-in-vitro-and-treating-drug-resistant-lung-cancer-mice-in-vivo
#14
Xueyan Zhang, Fang Hu, Changhui Li, Xiaoxuan Zheng, Bo Zhang, Huimin Wang, Guangyu Tao, Jianlin Xu, Yanwei Zhang, Baohui Han
This study aimed to investigate the synergistic effect of octamer-binding transcription factor 4 and sex determining region Y-box 2 (OCT4&SOX2)-specific cytotoxic T lymphocytes (CTLs) and programmed cell death protein 1 (PD-1) inhibitor on killing lung cancer stem-like cells (LCSCs) and their efficacy in treating drug-resistant lung cancer (DRLC) mice. OCT4&SOX2-specific CTLs and PD-1 inhibitor with differed doses were applied to treat PC9 cells and PC9 LCSCs. Cell counting kit-8 (CCK8) assay and flow cytometry (FCM) assay with carboxyfluorescein diacetate/succinimidyl ester staining target cells before treatment and propidium iodide (PI) staining dead cells after treatment were conducted to detect the cytotoxic activity...
November 1, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/30367941/structural-and-biochemical-analysis-of-a-not1-mif4g-like-domain-of-the-ccr4-not-complex
#15
Tobias Raisch, Felix Sandmeir, Oliver Weichenrieder, Eugene Valkov, Elisa Izaurralde
The CCR4-NOT complex plays a central role in the regulation of gene expression and degradation of messenger RNAs. The multisubunit complex assembles on the NOT1 protein, which acts as a 'scaffold' and is highly conserved in eukaryotes. NOT1 consists of a series of helical domains that serve as docking sites for other CCR4-NOT subunits. We describe a crystal structure of a connector domain of NOT1 from the thermophilic fungus Chaetomium thermophilum (Ct). Comparative structural analysis indicates that this domain adopts a MIF4G-like fold and we have termed it the MIF4G-C domain...
October 24, 2018: Journal of Structural Biology
https://www.readbyqxmd.com/read/30367141/identification-and-characterization-of-usp7-targets-in-cancer-cells
#16
Anna Georges, Edyta Marcon, Jack Greenblatt, Lori Frappier
The ubiquitin specific protease, USP7, regulates multiple cellular pathways relevant for cancer through its ability to bind and sometimes stabilize specific target proteins through deubiquitylation. To gain a more complete profile of USP7 interactions in cancer cells, we performed affinity purification coupled to mass spectrometry to identify USP7 binding targets in gastric carcinoma cells. This confirmed reported associations of USP7 with USP11, PPM1G phosphatase and TRIP12 E3 ubiquitin ligase as well as identifying novel interactions with two DEAD/DEAH-box RNA helicases, DDX24 and DHX40...
October 26, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30367066/a-fret-biosensor-for-necroptosis-uncovers-two-different-modes-of-the-release-of-damps
#17
Shin Murai, Yoshifumi Yamaguchi, Yoshitaka Shirasaki, Mai Yamagishi, Ryodai Shindo, Joanne M Hildebrand, Ryosuke Miura, Osamu Nakabayashi, Mamoru Totsuka, Taichiro Tomida, Satomi Adachi-Akahane, Sotaro Uemura, John Silke, Hideo Yagita, Masayuki Miura, Hiroyasu Nakano
Necroptosis is a regulated form of necrosis that depends on receptor-interacting protein kinase (RIPK)3 and mixed lineage kinase domain-like (MLKL). While danger-associated molecular pattern (DAMP)s are involved in various pathological conditions and released from dead cells, the underlying mechanisms are not fully understood. Here we develop a fluorescence resonance energy transfer (FRET) biosensor, termed SMART (a sensor for MLKL activation by RIPK3 based on FRET). SMART is composed of a fragment of MLKL and monitors necroptosis, but not apoptosis or necrosis...
October 26, 2018: Nature Communications
https://www.readbyqxmd.com/read/30365976/the-steroid-derivative-6-aminocholestanol-inhibits-the-dead-box-helicase-eif4a-lieif4a-from-the-trypanosomatid-parasite-leishmania-by-perturbing-the-rna-and-atp-binding-sites
#18
Yosser Zina Abdelkrim, Emna Harigua-Souiai, Mourad Barhoumi, Josette Banroques, Arnaud Blondel, Ikram Guizani, N Kyle Tanner
The antifungal agent 6-aminocholestanol targets the production of ergosterol, which is the principle sterol in many fungi and protozoans; ergosterol serves many of the same roles as cholesterol in animals. We found that it also is an effective inhibitor of the translation-initiation factor eIF4AI from mouse (eIF4AIMus ) and the Trypanosomatid parasite Leishmania (LieIF4A). The eIF4A proteins belong to the DEAD-box family of RNA helicases, which are ATP-dependent RNA-binding proteins and RNA-dependent ATPases...
October 23, 2018: Molecular and Biochemical Parasitology
https://www.readbyqxmd.com/read/30346046/dhh1-is-a-member-of-the-sesa-network
#19
Bengü Ergüden
The correct separation of chromosomes during mitosis is necessary to prevent genetic instability and aneuploidy, which are responsible for cancer and other diseases, and it depends on proper centrosome duplication. In a recent study, we found that Smy2 can suppress the essential role of Mps2 in the insertion of yeast centrosome into the nuclear membrane by interacting with Eap1, Scp160, and Asc1, and designated this network as SESA (Smy2, Eap1, Scp160, Asc1). Detailed analysis showed that the SESA network is part of a mechanism which regulates translation of POM34 mRNA...
October 22, 2018: Yeast
https://www.readbyqxmd.com/read/30341167/ddx3-directly-facilitates-ikk%C3%AE-activation-and-regulates-downstream-signalling-pathways
#20
Anthony Fullam, Lili Gu, Yvette Höhn, Martina Schröder
DDX3 is a DEAD-box RNA helicase that we and others have previously implicated in anti-viral immune signalling pathways leading to type I interferon (IFN) induction. We previously demonstrated that it directly interacts with the kinase IKKε, enhances it activation, and then facilitates phosphorylation of the transcription factor IRF3 by IKKε. However, the TLR7/9-mediated pathway, one of the most physiologically relevant IFN induction pathways, proceeds independently of IKKε or the related kinase TBK1. This pathway induces type I IFN production via the kinases NIK and IKKα and is activated when plasmacytoid Dendritic Cells (pDCs) sense viral nucleic acids...
October 19, 2018: Biochemical Journal
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