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https://www.readbyqxmd.com/read/29611334/conditional-ablation-of-the-prorenin-receptor-in-nephron-progenitor-cells-results-in-developmental-programming-of-hypertension
#1
Renfang Song, Laura Kidd, Adam Janssen, Ihor V Yosypiv
Nephron induction during kidney development is driven by reciprocal interactions between progenitor cells (NPCs) of the cap mesenchyme (CM) and the ureteric bud (UB). The prorenin receptor (PRR) is a receptor for renin and prorenin, and an accessory subunit of the vacuolar proton pump V-ATPase. Previously, we demonstrated that conditional ablation of the PRR in Six2+ NPCs in mice (Six2PRR -/- ) causes early neonatal death. Here, we identified genes that are regulated by PRR in Six2+ NPCs FACS-isolated from Six2PRR -/- and control kidneys on embryonic day E15...
April 2018: Physiological Reports
https://www.readbyqxmd.com/read/29559248/role-of-mir-452-5p-in-the-tumorigenesis-of-prostate-cancer-a-study-based-on-the-cancer-genome-atl-tcga-gene-expression-omnibus-geo-and-bioinformatics-analysis
#2
Li Gao, Li-Jie Zhang, Sheng-Hua Li, Li-Li Wei, Bin Luo, Rong-Quan He, Shuang Xia
BACKGROUND: MiR-452-5p has been reported to be down-regulated in prostate cancer, affecting the development of this type of cancer. However, the molecular mechanism of miR-452-5p in prostate cancer remains unclear. Therefore, we investigated the network of target genes of miR-452-5p in prostate cancer using bioinformatics analyses. MATERIALS AND METHODS: We first analyzed the expression profiles and prognostic value of miR-452-5p in prostate cancer tissues from a public database...
March 6, 2018: Pathology, Research and Practice
https://www.readbyqxmd.com/read/29555661/salivary-gland-cancer-patient-derived-xenografts-enable-characterization-of-cancer-stem-cells-and-new-gene-fusions-associated-with-tumor-progression
#3
Stephen Keysar, Justin Eagles, Bettina Miller, Brian C Jackson, Farshad N Chowdhury, Julie Reisinger, Tugs-Saikhan Chimed, Phuong N Le, J Jason Morton, Hilary Somerset, Marileila Varella-Garcia, Aik-Choon Tan, John I Song, Daniel W Bowles, Mary E Reyland, Antonio Jimeno
PURPOSE: Salivary gland cancers (SGC) frequently present with distant metastases many years after diagnosis, suggesting a cancer stem cell (CSC) subpopulation that initiates late recurrences; however current models are limited both in their availability and suitability to characterize these rare cells. EXPERIMENTAL DESIGN: Patient-derived xenografts (PDX) were generated by engrafting patient tissue onto nude mice from one acinic cell carcinoma (AciCC), four adenoid cystic carcinoma (ACC), and three mucoepidermoid carcinoma (MEC) cases, which were derived from successive relapses from the same MEC patient...
March 19, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/29551399/the-intestinal-stem-cell-regulating-gene-ascl2-is-required-for-l1-mediated-colon-cancer-progression
#4
Sayon Basu, Nancy Gavert, Thomas Brabletz, Avri Ben-Ze'ev
Aberrant Wnt/β-catenin signaling is a common event during human colorectal cancer (CRC) development. Previously, we characterized members of the L1 family of cell adhesion receptors as targets of β-catenin-LEF1/TCF transactivation that are expressed at the invasive CRC tissue edge. Overexpression of L1 in CRC cells confers enhanced motility, tumorigenesis and liver metastasis. We identified several downstream targets of L1-mediated signaling that are considered key intestinal stem cell signature genes. Here, we investigated the involvement of ASCL2, a Wnt target gene and key determinant of intestinal stem cell state, in L1-mediated CRC progression...
March 15, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29544705/gene-expression-profiling-of-low-grade-endometrial-stromal-sarcoma-indicates-fusion-protein-mediated-activation-of-the-wnt-signaling-pathway
#5
Joanna Przybyl, Lukasz Kidzinski, Trevor Hastie, Maria Debiec-Rychter, Roel Nusse, Matt van de Rijn
OBJECTIVE: Low-grade endometrial stromal sarcomas (LGESS) harbor chromosomal translocations that affect proteins associated with chromatin remodeling Polycomb Repressive Complex 2 (PRC2), including SUZ12, PHF1 and EPC1. Roughly half of LGESS also demonstrate nuclear accumulation of β-catenin, which is a hallmark of Wnt signaling activation. However, the targets affected by the fusion proteins and the role of Wnt signaling in the pathogenesis of these tumors remain largely unknown. METHODS: Here we report the results of a meta-analysis of three independent gene expression profiling studies on LGESS and immunohistochemical evaluation of nuclear expression of β-catenin and Lef1 in 112 uterine sarcoma specimens obtained from 20 LGESS and 89 LMS patients...
March 12, 2018: Gynecologic Oncology
https://www.readbyqxmd.com/read/29541960/the-role-of-sox9-in-maintaining-the-characteristics-and-pluripotency-of-arbas-cashmere-goat-hair-follicle-stem-cells
#6
Nimantana He, Zhenguo Dong, Dapeng Tai, Hao Liang, Xudong Guo, Ming Cang, Dongjun Liu
In our previous work, we isolated Arbas Cashmere goat hair follicle stem cells (gHFSCs) and explored the pluripotency. In this study, we investigated the expression and putative role of Sox9 in the gHFSCs. Immunofluorescence staining showed that Sox9 is predominantly expressed in the bulge region of the Arbas Cashmere goat hair follicle, and also positively expressed in both nucleus and cytoplasm of the gHFSCs. When the cells were transfected using Sox9-shRNA, cell growth slowed down and the expression of related genes decreased significantly, cell cycle was abnormal, while the expression of terminal differentiation marker loricrin was markedly increased; cells lost the typical morphology of HFSCs; the mRNA and protein expression of gHFSCs markers and stem cell pluripotency associated factors were all significantly decreased; the expression of Wnt signaling pathway genes LEF1, TCF1,c-Myc were significantly changed...
March 14, 2018: Cytotechnology
https://www.readbyqxmd.com/read/29535816/epstein-barr-virus-stably-confers-an-invasive-phenotype-to-epithelial-cells-through-reprogramming-of-the-wnt-pathway
#7
Christine E Birdwell, Kanchanjunga Prasai, Samantha Dykes, Yali Jia, Tawsha G C Munroe, Malgorzata Bienkowska-Haba, Rona S Scott
Epstein-Barr virus (EBV)-associated carcinomas, such as nasopharyngeal carcinoma (NPC), exhibit an undifferentiated and metastatic phenotype. To determine viral contributions involved in the invasive phenotype of EBV-associated carcinomas, EBV-infected human telomerase-immortalized normal oral keratinocytes (NOK) were investigated. EBV-infected NOK were previously shown to undergo epigenetic reprogramming involving CpG island hypermethylation and delayed responsiveness to differentiation. Here, we show that EBV-infected NOK acquired an invasive phenotype that was epigenetically retained after viral loss...
February 13, 2018: Oncotarget
https://www.readbyqxmd.com/read/29520849/lncrna-h19-targets-mir-22-to-modulate-h-2-o-2-induced-deregulation-in-nucleus-pulposus-cell-senescence-proliferation-and-ecm-synthesis-through-wnt-signaling
#8
Xiaobin Wang, Mingxiang Zou, Jing Li, Bing Wang, Qianshi Zhang, Fubin Liu, Guohua Lü
Intervertebral disc (IVD) degeneration (IDD) is a major contributor to low back pain. During IDD progression, ROS can be produced in the form of H2 O2 in nucleus pulposus cells (NPCs) in response to elevated cytokines, leading to subsequent alternations of cell fate and metabolic processes. Genetic factors are considered as main contributors to IDD pathopoiesis. Herein, we investigated the detailed function and mechanism of H19, one of the most up-regulated lncRNAs in IDD specimens, in H2 O2 -induced cell senescence model in NPCs...
March 9, 2018: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29483204/mir-103-107-promote-er-stress-mediated-apoptosis-via-targeting-the-wnt3a-%C3%AE-catenin-atf6-pathway-in-preadipocytes
#9
Zhenzhen Zhang, Song Wu, Saeed Muhammad, Qian Ren, Chao Sun
Both miR-103 and 107 have been demonstrated to restrain cell proliferation, regulate lipid metabolism and inflammation. However, the effects of miR-103/107 on preadipocytes apoptosis remain unknown. In the present research, we have investigated how miR-103/107 regulated preadipocytes apoptosis. We found that miR-103/107 aggravated Endoplasmic Reticulum (ER) stress mediated apoptosis in preadipocytes. We confirmed that miR-103/107 targeted Wnt3a (WNT family member 3a) in preadipocytes. It was found that overexpressing Wnt3a resulted in suppression of ER stress mediated apoptosis, while restoration of miR-103/107 counteracted the effects of Wnt3a in preadipocytes...
February 26, 2018: Journal of Lipid Research
https://www.readbyqxmd.com/read/29481312/upstream-enhancer-elements-of-shh-regulate-oral-and-dental-patterning
#10
H Seo, T Amano, R Seki, T Sagai, J Kim, S W Cho, T Shiroishi
Sonic hedgehog ( Shh) is important in pattern formation during development. Shh transcription is modulated by a long-range regulatory mechanism containing a number of enhancers, which are spread over nearly 850 kb in the mouse genome. Shh enhancers in the nervous system have been found between intron and 430 kb upstream of Shh. Enhancers in the oral cavity, pharynx, lung, gut, and limbs have been discovered between 610 kb and 850 kb upstream of Shh. However, the intergenic region ranging from 430 to 610 kb upstream of Shh remains to be elucidated...
February 1, 2018: Journal of Dental Research
https://www.readbyqxmd.com/read/29472529/a-novel-microrna-identified-in-hepatocellular-carcinomas-is-responsive-to-lef1-and-facilitates-proliferation-and-epithelial-mesenchymal-transition-via-targeting-of-nfix
#11
Yaqi Hu, Xu Guo, Jinxia Wang, Yankun Liu, Huijie Gao, Hongxia Fan, Xiangyang Nong, Xi Yang, Min Liu, Shengping Li, Hua Tang
Hepatocellular carcinoma (HCC) is one of the most prevalent cancers. It has been demonstrated that various cellular microRNAs (miRNAs) play an important role in HCC development. Here, we analyzed the miRNA profile in HCC tissues by Solexa sequencing, and we identified a novel microRNA, miR-HCC1, which is upregulated in HCC tissues. Further experiments showed that miR-HCC1 promoted HCC cell proliferation in vivo and in vitro, and migration and invasion resulting from the epithelial-mesenchymal transition (EMT) process...
February 23, 2018: Oncogenesis
https://www.readbyqxmd.com/read/29468283/activation-of-wnt-%C3%AE-catenin-signaling-is-involved-in-hair-growth-promoting-effect-of-655-nm-red-light-and-led-in-in-vitro-culture-model
#12
Le Han, Ben Liu, Xianyan Chen, Haiyan Chen, Wenjia Deng, Changsheng Yang, Bin Ji, Miaojian Wan
Activation of the Wnt/β-catenin signaling pathway plays an important role in hair follicle morphogenesis and hair growth. Recently, low-level laser therapy (LLLT) was evaluated for stimulating hair growth in numerous clinical studies, in which 655-nm red light was found to be most effective and practical for stimulating hair growth. We evaluated whether 655-nm red light + light-emitting diode (LED) could promote human hair growth by activating Wnt/β-catenin signaling. An in vitro culture of human hair follicles (HFs) was irradiated with different intensities of 655-nm red light + LED, 21 h7 (an inhibitor of β-catenin), or both...
February 21, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/29467848/differential-microrna-expression-in-the-prefrontal-cortex-of-mouse-offspring-induced-by-glyphosate-exposure-during-pregnancy-and-lactation
#13
Hua Ji, Linhao Xu, Zheng Wang, Xinli Fan, Lihui Wu
Glyphosate is the active ingredient in numerous herbicide formulations. The role of glyphosate in neurotoxicity has been reported in human and animal models. However, the detailed mechanism of the role of glyphosate in neuronal development remains unknown. Recently, several studies have reported evidence linking neurodevelopmental disorders (NDDs) with gestational glyphosate exposure. The current group previously identified microRNAs (miRNAs) that are associated with the etiology of NDDs, but their expression levels in the developing brain following glyphosate exposure have not been characterized...
March 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29462785/the-plant-sesquiterpene-lactone-parthenoide-inhibits-wnt-%C3%AE-catenin-signaling-by-blocking-synthesis-of-the-transcriptional-regulators-tcf4-lef1
#14
Xiaoliang Zhu, Chunmao Yuan, Chenyang Tian, Chen Li, Fen Nie, Xiaomin Song, Rong Zeng, Dianqing Wu, Xiaojiang Hao, Lin Li
The Wnt/β-catenin pathway is essential for embryonic development and homeostasis, but excessive activation of this pathway is frequently observed in various human diseases, including cancer. Current therapeutic drugs targeting the Wnt pathway often lack sufficient efficacy, and new compounds targeting this pathway are therefore greatly needed. Here, we report that the plant-derived natural product parthenoide (PTL), a sesquiterpene lactone, inhibits Wnt signaling. We found that PTL dose-dependently inhibits Wnt3a- and CHIR-induced transcriptional activity assessed with the T-cell factor/lymphoid enhancer factor(TCF/LEF)-firefly luciferase (TOPFlash) assay in HEK293 cells...
February 9, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29439547/prostaglandin-d2-mediated-dp2-and-akt-signal-regulate-the-activation-of-androgen-receptors-in-human-dermal-papilla-cells
#15
Kwan Ho Jeong, Ji Hee Jung, Jung Eun Kim, Hoon Kang
Prostaglandin D2 (PGD2) and prostaglandin D2 receptor 2 (DP2) is known to be an important factor in androgenetic alopecia (AGA). However, the effect of PGD2 in human dermal papilla cells (hDPCs) is not fully understood. The function of PGD2-induced expression of the androgen receptor (AR), DP2, and AKT (protein kinase B) signal were examined by using real time-PCR (qRT-PCR), western blot analysis, immunocytochemistry (ICC), and siRNA transfection system. PGD2 stimulated AR expression and AKT signaling through DP2...
February 12, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29432853/neurobehavioral-effects-of-1-2-propanediol-in-zebrafish-danio-rerio
#16
Andrey Massarsky, Ayham Abdel, Lilah Glazer, Edward D Levin, Richard T Di Giulio
The use of electronic cigarettes (e-cigarettes) is increasing despite insufficient information concerning their long-term effects, including the effects of maternal e-cigarette use on pre- and postnatal development. Our previous study demonstrated that developmental exposure to 1,2-propanediol (a principal component of e-cigarette liquid) affected early development of zebrafish, causing reduced growth, deformities, and hyperactive swimming behavior in larvae. The current study extends assessment of the developmental toxicity of 1,2-propanediol by examining additional long-term behavioral effects...
February 9, 2018: Neurotoxicology
https://www.readbyqxmd.com/read/29411534/extracellular-vesicles-have-variable-dose-dependent-effects-on-cultured-draining-cells-in-the-eye
#17
Saray Tabak, Sofia Schreiber-Avissar, Elie Beit-Yannai
The role of extracellular vesicles (EVs) as signal mediators has been described in many biological fields. How many EVs are needed to deliver the desired physiological signal is yet unclear. Using a normal trabecular meshwork (NTM) cell culture exposed to non-pigmented ciliary epithelium (NPCE)-derived EVs, a relevant model for studying the human ocular drainage system, we addressed the EVs dose-response effects on the Wnt signaling. The objective of the study was to investigate the dosing effects of NPCE-derived EVs on TM Wnt signaling...
February 7, 2018: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/29410176/transient-activation-of-wnt-%C3%AE-catenin-signaling-reporter-in-fibrotic-scar-formation-after-compression-spinal-cord-injury-in-adult-mice
#18
Takashi Yamagami, David E Pleasure, Kit S Lam, Chengji J Zhou
After traumatic spinal cord injury (SCI), a scar may form with a fibrotic core (fibrotic scar) and surrounding reactive astrocytes (glial scar) at the lesion site. The scar tissue is considered a major obstacle preventing regeneration both as a physical barrier and as a source for secretion of inhibitors of axonal regeneration. Understanding the mechanism of scar formation and how to control it may lead to effective SCI therapies. Using a compression-SCI model on adult transgenic mice, we demonstrate that the canonical Wnt/β-catenin signaling reporter TOPgal (TCF/Lef1-lacZ) positive cells appeared at the lesion site by 5 days, peaked on 7 days, and diminished by 14 days post injury...
February 2, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29408622/inhibition-of-hepatocellular-carcinoma-tumorigenesis-by-curcumin-may-be-associated-with-cdkn1a-and-ctgf
#19
Yun Zeng, Zhengjie Shen, Wenzhe Gu, Mianhua Wu
This study aimed to explore crucial genes, transcription factors (TFs), and microRNAs (miRNAs) associated with the effects of curcumin against hepatocellular carcinoma (HCC). We downloaded data (GSE59713) from Gene Expression Omnibus to analyze differentially expressed genes (DEGs) between curcumin-treated and untreated HCC cell lines. Then, we identified the disease ontology (DO) and functional enrichment analysis of these DEGs and analyzed their protein-protein interactions (PPIs). Additionally, we constructed TF-target gene and miRNA-target gene regulatory networks and explored the potential functions of these DEGs...
February 2, 2018: Gene
https://www.readbyqxmd.com/read/29362488/transcriptional-regulation-of-p63-on-the-apoptosis-of-male-germ-cells-and-three-stages-of-spermatogenesis-in-mice
#20
Hong Wang, Qingqing Yuan, Minghui Niu, Wenhui Zhang, Liping Wen, Hongyong Fu, Fan Zhou, Zuping He
Infertility affects 10-15% of couples worldwide, and male factors account for 50%. Spermatogenesis is precisely regulated by genetic factors, and the mutations of genes result in abnormal spermatogenesis and eventual male infertility. The aim of this study was to explore the role and transcriptional regulation of P63 in the apoptosis and mouse spermatogenesis. P63 protein was decreased in male germ cells of P63(+/-) mice compared with wild-type mice. There was no obvious difference in testis weight, sperm motility, and fecundity between P63(+/-) and wild-type mice...
January 23, 2018: Cell Death & Disease
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