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https://www.readbyqxmd.com/read/29149771/hoxc13-and-hsp27-expression-in-skin-and-the-periodic-growth-of-secondary-fiber-follicles-from-longdong-cashmere-goats-raised-in-different-production-systems
#1
Jiqing Wang, Ping Yu, Haifang Wang, Yanyu He
This experiment was conducted to identify periodic changes in secondary follicles (SFs) over one year of extensive (grazed) and intensive (housed and fed supplement) farming of Longdong cashmere goats. One-year old goats (n = 24, 12 extensively fed and 12 intensively fed) were studied. The diameter, depth, density, activity, and ultra-structural features of SFs were assessed using light microscopy and transmission electron microscopy (TEM). HOXC13 and HSP27 expression were studied using immunohistochemistry and immunofluorescence method...
November 17, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/29123134/divergent-proliferation-patterns-of-distinct-human-hair-follicle-epithelial-progenitor-niches-in-situ-and-their-differential-responsiveness-to-prostaglandin-d2
#2
Talveen S Purba, Michael Peake, Bessam Farjo, Nilofer Farjo, Ranjit K Bhogal, Gail Jenkins, Ralf Paus
Human scalp hair follicles (hHF) harbour several epithelial stem (eHFSC) and progenitor cell sub-populations organised into spatially distinct niches. However, the constitutive cell cycle activity of these niches remains to be characterized in situ. Therefore, the current study has studied these characteristics of keratin 15+ (K15), CD200+ or CD34+ cells within anagen VI hHFs by immunohistomorphometry, using Ki-67 and 5-ethynyl-2'-deoxyuridine (EdU). We quantitatively demonstrate in situ the relative cell cycle inactivity of the CD200+/K15+ bulge compared to other non-bulge CD34+ and K15+ progenitor compartments and found that in each recognized eHFSC/progenitor niche, proliferation associates negatively with eHFSC-marker expression...
November 9, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29115640/off%C3%A2-target-effect-of-imatinib-and-nilotinib-on-human-vitamin-d3-metabolism
#3
Lysann Kroschwald, Meinolf Suttorp, Josephine Tabea Tauer, Nick Zimmermann, Claudia Günther, Andrea Bauer
Prolonged treatment with tyrosine kinase inhibitors (TKI) including imatinib (IMA) or nilotinib (NIL), induces severe disturbances of bone metabolism in patients with chronic myeloid leukaemia. As vitamin D3 (VD3) is involved in the complex cycle of bone remodelling, the present study investigated in vitro, the influence of IMA and NIL on VD3 metabolism i) in HaCaT cells and ii) in cultured outer root sheath keratinocytes (ORS‑KC) from hair follicles of IMA treated children. Cells were incubated in the presence of IMA or NIL...
November 3, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29078349/requirement-of-zinc-transporter-zip10-for-epidermal-development-implication-of-the-zip10-p63-axis-in-epithelial-homeostasis
#4
Bum-Ho Bin, Jinhyuk Bhin, Mikiro Takaishi, Koh-Ei Toyoshima, Saeko Kawamata, Kana Ito, Takafumi Hara, Takashi Watanabe, Tarou Irié, Teruhisa Takagishi, Su-Hyon Lee, Haeng-Sun Jung, Sangchul Rho, Juyeon Seo, Dong-Hwa Choi, Daehee Hwang, Haruhiko Koseki, Osamu Ohara, Shigetoshi Sano, Takashi Tsuji, Kenji Mishima, Toshiyuki Fukada
Skin tissues, in particular the epidermis, are severely affected by zinc deficiency. However, the zinc-mediated mechanisms that maintain the cells that form the epidermis have not been established. Here, we report that the zinc transporter ZIP10 is highly expressed in the outer root sheath of hair follicles and plays critical roles in epidermal development. We found that ZIP10 marked epidermal progenitor cell subsets and that ablating Zip10 caused significant epidermal hypoplasia accompanied by down-regulation of the transactivation of p63, a master regulator of epidermal progenitor cell proliferation and differentiation...
October 23, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29030089/knockdown-of-hif-1%C3%AE-inhibits-the-proliferation-and-migration-of-outer-root-sheath-cells-exposed-to-hypoxia-in-vitro-an-involvement-of-shh-pathway
#5
Haihua Zhang, Weixiao Nan, Xingchao Song, Shiyong Wang, Huazhe Si, Guangyu Li
AIMS: Outer root sheath (ORS) is a highly proliferative component of a hair follicle. This study is performed to investigate whether hypoxia-induced elevation of hypoxia-inducible factor (HIF)-1α, a transcriptional activator, contributes to the outgrowth of ORS cells in vitro. MAIN METHODS: Hair follicles with intact ORS collected from 4-month old male American minks were cultured in normoxic or hypoxic condition (3% oxygen) for 7days. Primary ORS cells isolated from the mink hair follicles were exposed to hypoxia for 12, 24 or 48h, and their proliferation was analyzed with immunofluorescence assay using anti-proliferating cell nuclear antigen (PCNA) antibody...
October 10, 2017: Life Sciences
https://www.readbyqxmd.com/read/28979862/comparison-of-direct-immunofluorescence-of-plucked-hair-and-skin-for-evaluation-of-immunological-remission-in-pemphigus
#6
Reena Rai, Manu V Harikumar
BACKGROUND: Pemphigus is a chronic autoimmune bullous disorder characterized by autoantibodies directed against desmoglein 3 and/or 1. Demonstration of intercellular deposition of IgG on the cell surface of keratinocytes by direct immunofluorescence (DIF) of the skin is the gold standard in the diagnosis of pemphigus. Recently, DIF of plucked hair demonstrating intercellular deposition of IgG in the outer root sheath (ORS) has shown to be useful. OBJECTIVE: To compare the DIF of plucked hair and skin for the evaluation of immunological remission in pemphigus vulgaris patients in clinical remission...
September 2017: Indian Dermatology Online Journal
https://www.readbyqxmd.com/read/28971483/study-of-efficacy-and-safety-of-noncultured-extracted-follicular-outer-root-sheath-cell-suspension-transplantation-in-the-management-of-stable-vitiligo
#7
Pawan Kumar, Neetu Bhari, Manoj K Tembhre, Sujata Mohanty, Sudheer Arava, Vinod K Sharma, Somesh Gupta
BACKGROUND: Noncultured, extracted follicular outer root sheath suspension (NC-EHF-ORS-CS) is a recently introduced technique for the treatment of stable vitiligo. OBJECTIVE: To study the clinical efficacy of this technique and to determine the viability and cell composition of the suspension. METHODS: Twenty-five patients with stable vitiligo were included in this prospective study. Fifty follicles were extracted from occipital scalp and were incubated with trypsin-ethylenediaminetetraacetic acid to separate outer root sheath cells...
October 3, 2017: International Journal of Dermatology
https://www.readbyqxmd.com/read/28960405/expression-of-connexin43-cx43-in-the-human-hair-follicle-emphasis-on-the-cx43-protein-levels-in-the-bulge-and-through-the-keratinization-process
#8
A Fernandez-Flores, A Varela-Vazquez, M D Mayan, E Fonseca
INTRODUCTION: Gap junctions form communication compartments between cells. These channels assemble from connexin (Cx) subunits. OBJECTIVE: To investigate the immunoexpression of Cx43 in adult human hair follicles. MATERIALS AND METHODS: Cases were retrospectively obtained from our archives. RESULTS: We identified immunoexpression of Cx43 in the matrix, the papilla, the outer root sheath, the bulge, the medulla, the cortex, the shaft, and the secretory part of the sebaceous gland...
September 28, 2017: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/28944964/wnt-%C3%AE-catenin-and-erk-pathway-activation-a-possible-mechanism-of-photobiomodulation-therapy-with-light-emitting-diodes-that-regulate-the-proliferation-of-human-outer-root-sheath-cells
#9
Jung E Kim, Young J Woo, Ki M Sohn, Kwan H Jeong, Hoon Kang
BACKGROUND: Outer root sheath cells (ORSCs) play important roles in maintaining hair follicle structure and provide support for the bulge area. The hair growth promoting effects of photobiomodulation therapy (PBMT) have been reported, but the mechanisms for this in human ORCs (hORSCs) have rarely been studied. OBJECTIVE: The aim of this study was to investigate the effect of various wavelengths of light-emitting diode (LED) irradiation on human ORSCs (hORSCs). METHODS: LED irradiation effects on hORSC proliferation and migration were examined with MTT assay, BrdU incorporation assay and migration assays...
September 25, 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/28939680/mobilizing-transit-amplifying-cell-derived-ectopic-progenitors-prevents-hair-loss-from-chemotherapy-or-radiation-therapy
#10
Wen-Yen Huang, Shih-Fan Lai, Hsien-Yi Chiu, Michael Chang, Maksim V Plikus, Chih-Chieh Chan, You-Tzung Chen, Po-Nien Tsao, Tsung-Lin Yang, Hsuan-Shu Lee, Peter Chi, Sung-Jan Lin
Genotoxicity-induced hair loss from chemotherapy and radiotherapy is often encountered in cancer treatment, and there is a lack of effective treatment. In growing hair follicles (HF), quiescent stem cells (SC) are maintained in the bulge region, and hair bulbs at the base contain rapidly dividing, yet genotoxicity-sensitive transit-amplifying cells (TAC) that maintain hair growth. How genotoxicity-induced HF injury is repaired remains unclear. We report here that HFs mobilize ectopic progenitors from distinct TAC compartments for regeneration in adaptation to the severity of dystrophy induced by ionizing radiation (IR)...
September 22, 2017: Cancer Research
https://www.readbyqxmd.com/read/28914451/pilomatrix-carcinoma-a-10-year-review-of-the-literature
#11
REVIEW
Christopher Jones, Mark Twoon, Weiguang Ho, Mark Portelli, Bernard Robertson, William Anderson
Pilomatrixoma, also known as calcifying epithelioma of Malherbe, is a superficial benign skin tumour arising from hair follicle matrix cells (1,2) usually located in the head or neck region (1). It was first described in 1880 by Malherbe and Chenantais as a calcified epithelioma of sebaceous origin. In 1961, Forbis and Helwig proposed the term pilomatrixoma after establishing that the lesion actually originated from the outer root sheath cell of hair follicles (3-5).
September 15, 2017: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/28857383/a-human-serum-enriched-medium-formulation-supports-high-viability-and-marker-expression-in-primary-melanocyte-cultures-from-the-outer-root-sheath-and-epidermis
#12
Marie Schneider, Andrea Lohrenz, Michael Cross, Michael C Hacker, Jan C Simon, Vuk Savkovic
Formulating clinically relevant melanocyte cultivation media that maintain the balance between proliferation and maturation to functional melanocytes is a major experimental and regulatory challenge. Within the translation of human melanocytes from the outer root sheath of human hair follicle (HUMORS), we developed a melanocyte medium free of chemical mitogens, chemical melanogenesis enhancers and bovine products, enabling proliferation as well as melanotic differentiation. The formulation involved the replacement of bovine pituitary extract (BPE) and bovine serum (FBS) with human serum (HS) combined with ascorbic acid, CaCl2, epinephrine, L glutamine, insulin and fibroblast growth factor...
August 31, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28849028/panax-ginseng-extract-antagonizes-the-effect-of-dkk%C3%A2-1-induced-catagen-like-changes-of-hair-follicles
#13
Yonghee Lee, Su Na Kim, Yong Deog Hong, Byung Cheol Park, Yongjoo Na
It is well known that Panax ginseng (PG) has various pharmacological effects such as anti-aging and anti-inflammation. In a previous study, the authors identified that PG extract induced hair growth by means of a mechanism similar to that of minoxidil. In the present study, the inhibitory effect of PG extract on Dickkopf-1 (DKK-1)-induced catagen-like changes in hair follicles (HFs) was investigated in addition to the underlying mechanism of action. The effects of PG extract on cell proliferation, anti-apoptotic effect, and hair growth were observed using cultured outer root sheath (ORS) keratinocytes and human HFs with or without DKK-1 treatment...
October 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/28809777/pigmented-epithelioid-melanocytoma-pem-animal-type-melanoma-atm-quest-for-an-origin-report-of-one-unusual-case-indicating-follicular-origin-and-another-arising-in-an-intradermal-nevus
#14
Ashley Tarasen, J Andrew Carlson, M Kathryn Leonard, Glenn Merlino, David Kaetzel, Andrzej T Slominski
Pigmented epithelioid melanocytoma (PEM) is a tumor encompassing epithelioid blue nevus of Carney complex (EBN of CNC) and was previously termed animal-type melanoma. Histologically PEMs are heavily pigmented spindled and epithelioid dermal melanocytic tumors with infiltrative borders, however, their origin remains unclear. Stem cells for the epidermis and hair follicle are located in the bulge area of the hair follicle with the potential to differentiate into multiple lineages. Multiple cutaneous carcinomas, including follicular cutaneous squamous cell carcinoma (FSCC), are thought to arise from stem cells in the follicular bulge...
August 15, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/28681425/localization-of-relm-%C3%AE-fizz2-is-associated-with-cementum-formation
#15
Akihiro Hosoya, Akira Takahama, Hiroaki Nakamura
Resistin-like molecule-β/found in inflammatory zone 2 (RELM-β/FIZZ2) is a cysteine-rich secretory protein that is localized in the epithelium of the gastrointestinal tract and lung alveoli. Previous reports have suggested that this protein regulates glucose metabolism and inflammation. In the present study, to analyze the involvement of RELM-β/FIZZ2 in tooth development, we immunohistochemically examined the localization of RELM-β/FIZZ2 in tooth germs of embryonic days (E) 15-20 and postnatal days (P) 7-42 rats...
July 6, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
https://www.readbyqxmd.com/read/28632917/miniaturization-of-sebaceous-glands-a-novel-histopathological-finding-in-pemphigus-vulgaris-and-pemphigus-foliaceus-of-the-scalp
#16
Marta Sar-Pomian, Joanna Czuwara, Lidia Rudnicka, Malgorzata Olszewska
BACKGROUND: Intraepidermal acantholysis is a characteristic histopathological feature of pemphigus. The histopathology of scalp biopsies in pemphigus has not been widely discussed in the literature, although the scalp is commonly involved in pemphigus. METHODS: A total of 32 consecutive patients with scalp involvement (17 with pemphigus vulgaris [PV] and 15 with pemphigus foliaceus [PF]) were enrolled into the study. The histopathological examination of scalp biopsies was performed in all patients...
June 20, 2017: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/28632903/immunohistochemical-dissection-of-cystic-panfolliculoma-focusing-on-the-expression-of-multiple-hair-follicle-lineage-markers-with-an-insight-into-the-pathogenesis
#17
Masahiro Fukuyama, Yohei Sato, Yoshimi Yamazaki, Manabu Ohyama
Panfolliculoma (PF) is a rare benign tumor with signs of differentiation toward all components of the hair follicle (HF). Cystic panfolliculoma (CPF) is a subset of PF with histological similarity to trichofolliculoma making the differential diagnosis difficult in some cases. Immunohistopathological investigations of PF have been reported; however, previous studies focused mostly on the expression profile of the outer root sheath markers. Herein, we report a case of CPF. A panel of antibodies was developed and used for the detection of the expression of cytokeratin (CK) 10, CK13, CK14, CK15, hair-hard keratin (AE13) and EpCAM within the lesion, supporting the differentiation of all epithelial lineages of the HF and the diagnosis of CPF...
June 20, 2017: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/28609346/plaque-like-pilar-sheath-acanthoma-histopathologic-and-immunohistochemical-study-of-3-unusual-cases
#18
Margarita Jo-Velasco, Araceli Corrales-Rodríguez, Laura Francés-Rodríguez, Victoria Alegría-Landa, Itziar Eraña-Tomás, Arno Rütten, Luis Requena
Pilar sheath acanthoma is an uncommon, benign follicular neoplasm that frequently presents as a solitary lesion. This neoplasm usually appears on the skin around the upper lip of elderly patients. Histopathologically, the neoplasm usually shows a cystic configuration with epithelial lobules resembling to those of the outer root sheath of the hair follicle at the level of the isthmus emanating radially from the cyst wall. We present 3 peculiar cases of a pilar sheath acanthoma showing a plaque-like architecture because the lesions exhibited a horizontal configuration...
June 6, 2017: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/28601433/characterization-of-xenobiotic-metabolizing-enzymes-of-a-reconstructed-human-epidermal-model-from-adult-hair-follicles
#19
Daniel Bacqueville, Carine Jacques, Laure Duprat, Emilien L Jamin, Beatrice Guiraud, Elisabeth Perdu, Sandrine Bessou-Touya, Daniel Zalko, Hélène Duplan
In this study, a comprehensive characterization of xenobiotic metabolizing enzymes (XMEs) based on gene expression and enzyme functionality was made in a reconstructed skin epidermal model derived from the outer root sheath (ORS) of hair follicles (ORS-RHE). The ORS-RHE model XME gene profile was consistent with native human skin. Cytochromes P450 (CYPs) consistently reported to be detected in native human skin were also present at the gene level in the ORS-RHE model. The highest Phase I XME gene expression levels were observed for alcohol/aldehyde dehydrogenases and (carboxyl) esterases...
August 15, 2017: Toxicology and Applied Pharmacology
https://www.readbyqxmd.com/read/28587354/th1-th2-pb-balance-and-cd200-expression-of-patients-with-active-severe-alopecia-areata
#20
Xinhua Ma, Shisheng Chen, Wanwan Jin, Yu Gao
Th1/Th2 peripheral blood balance and CD200 expression in patients with severe alopecia areata (SAA) in the active stage were investigated. Fifty patients with active SAA, 50 patients with stable SAA and 50 healthy controls were continuously selected and expression of Th1/Th2 of peripheral T lymphocytes, and peripheral B lymphocytes was detected by flow cytometry; RT-PCR was used to detect the expression of the PBMC CD200 mRNA and the expression of CD200 in hair follicles of alopecia area was detected by immunohistochemically staining; ELISA was used to detect expression levels of serum LFN-γ and serum interleukin (IL)-10...
June 2017: Experimental and Therapeutic Medicine
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