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https://www.readbyqxmd.com/read/28418107/a-new-path-in-defining-light-parameters-for-hair-growth-discovery-and-modulation-of-photoreceptors-in-human-hair-follicle
#1
Serena Buscone, Andrei N Mardaryev, Bianca Raafs, Jan W Bikker, Carsten Sticht, Norbert Gretz, Nilofer Farjo, Natallia E Uzunbajakava, Natalia V Botchkareva
BACKGROUND AND OBJECTIVE: Though devices for hair growth based on low levels of light have shown encouraging results, further improvements of their efficacy is impeded by a lack of knowledge on the exact molecular targets that mediate physiological response in skin and hair follicle. The aim of this study was to investigate the expression of selected light-sensitive receptors in the human hair follicle and to study the impact of UV-free blue light on hair growth ex vivo. MATERIAL AND METHODS: The expression of Opsin receptors in human skin and hair follicles has been characterized using RT-qPCR and immunofluorescence approaches...
April 18, 2017: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/28394798/sox10-and-dog1-expression-in-primary-adnexal-tumors-of-the-skin
#2
Cecilia Lezcano, Jonhan Ho, Raja R Seethala
Primary skin adnexal tumors can be challenging to classify and must be discerned from cutaneous adenocarcinoma metastases from various sites. We evaluated expression of Sox10 and DOG1 in normal cutaneous adnexa and in 194 primary skin adnexal tumors, and compared their performance in discriminating primary skin adnexal tumors from cutaneous metastatic adenocarcinomas with that of p40 and p63. In normal skin adnexa, we noted Sox10 expression in both the secretory and myoepithelial cells in eccrine glands, but only in myoepithelial cells in apocrine glands...
March 27, 2017: American Journal of Dermatopathology
https://www.readbyqxmd.com/read/28350869/in-vitro-skin-models-to-study-epithelial-regeneration-from-the-hair-follicle
#3
Nkemcho Ojeh, Baki Akgül, Marjana Tomic-Canic, Mike Philpott, Harshad Navsaria
The development of dermal equivalents (DEs) for the treatment of burns has contributed toward efficient wound closure. A collagen-glycosaminoglycan DE (C-GAG) grafted with hair follicles converted a full-thickness wound to partial-thickness resulting in complete wound closure and restored hair. In this study we compared the ability of both intact pilosebaceous units (PSU) or truncated hair follicles (THF) to regenerate a multilayered epidermis in vitro when implanted into de-epidermalized dermis (DED) or C-GAG with the epidermis generated in vivo using C-CAG...
2017: PloS One
https://www.readbyqxmd.com/read/28344061/compartmentation-of-mitochondrial-and-oxidative-metabolism-in-growing-hair-follicles-a-ring-of-fire
#4
John J Lemasters, Venkat K Ramshesh, Gregory L Lovelace, John Lim, Graham D Wright, Duane Harland, Thomas L Dawson
Little is known about the energetics of growing hair follicles, particularly in the mitochondrially abundant bulb. Here, mitochondrial and oxidative metabolism was visualized by multiphoton and light sheet microscopy in cultured bovine hair follicles and plucked human hairs. Mitochondrial membrane potential (ΔΨ), cell viability, reactive oxygen species (ROS) and secretory granules were assessed with parameter-indicating fluorophores. In growing follicles, lower bulb epithelial cells had high viability, and mitochondria were polarized...
March 23, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28329569/linear-trichoepithelioma-on-the-neck-of-a-15-year-old-girl
#5
Amanda J Laska, Roberto A Belli, Todd T Kobayashi
Trichoepitheliomas are trichogenic tumors that can have various clinical morphologies. These tumors are benign and differentiate toward the outer root sheath of the hair follicle. Solitary trichoepitheliomas arise sporadically, in contrast to multiple trichoepitheliomas, which are usually inherited as an autosomal dominant trait or as part of various genetic syndromes. We report a case of an adolescent female with a linear array of trichoepitheliomas on her left neck.
November 15, 2016: Dermatology Online Journal
https://www.readbyqxmd.com/read/28324846/novel-alk5-inhibitor-tp0427736-reduces-tgf-%C3%AE-%C3%A3-induced-growth-inhibition-in-human-outer-root-sheath-cells-and-elongates-anagen-phase-in-mouse-hair-follicles
#6
Takumi Naruse, Mari Aoki, Natsuko Fujimoto, Seiji Arase, Hajimu Oura, Yasuji Ueda, Akiko Ikeda
BACKGROUND: Androgenic alopecia (AGA) occurs as a result of the contraction of the anagen phase because of the action of androgens on hair follicles. TGF-β production from dermal papillae is enhanced by androgens, and growth inhibition of hair-follicle cells is induced by TGF-β, and the hair cycle progresses from the anagen phase to the catagen phase. We investigated both the in vitro and in vivo potency of the newly identified ALK5 inhibitor TP0427736 {6-[4-(4-methyl-1,3-thiazol-2-yl)-1H-imidazol-5-yl]-1,3-benzothiazole}...
January 25, 2017: Pharmacological Reports: PR
https://www.readbyqxmd.com/read/28266599/double-stranded-rna-induces-inflammation-via-the-nf-%C3%AE%C2%BAb-pathway-and-inflammasome-activation-in-the-outer-root-sheath-cells-of-hair-follicles
#7
Jung-Min Shin, Dae-Kyoung Choi, Kyung-Cheol Sohn, Soo-Yeon Kim, Jeong Min Ha, Young Ho Lee, Myung Im, Young-Joon Seo, Chang Deok Kim, Jeung-Hoon Lee, Young Lee
Alopecia areata (AA), a chronic, relapsing, hair-loss disorder, is considered to be a T cell-mediated autoimmune disease. It affects approximately 1.7% of the population, but its precise pathogenesis remains to be elucidated. Despite the recent attention focused on the roles of inflammasomes in the pathogenesis of autoinflammatory diseases, little is known about inflammasome activation in AA. Thus, in this study, we investigated the pattern of NLRP3 inflammasome activation in the outer root sheath (ORS) cells of hair follicles...
March 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28244687/vegf165-induces-differentiation-of-hair-follicle-stem-cells-into-endothelial-cells-and-plays-a-role-in-in-vivo-angiogenesis
#8
Renfu Quan, Weibin Du, Xuan Zheng, Shichao Xu, Qiang Li, Xing Ji, Ximei Wu, Rongxue Shao, Disheng Yang
Within the vascular endothelial growth factor (VEGF) family of five subtypes, VEGF165 secreted by endothelial cells has been identified to be the most active and widely distributed factor that plays a vital role in courses of angiogenesis, vascularization and mesenchymal cell differentiation. Hair follicle stem cells (HFSCs) can be harvested from the bulge region of the outer root sheath of the hair follicle and are adult stem cells that have multi-directional differentiation potential. Although the research on differentiation of stem cells (such as fat stem cells and bone marrow mesenchymal stem cells) to the endothelial cells has been extensive, but the various mechanisms and functional forms are unclear...
February 28, 2017: Journal of Cellular and Molecular Medicine
https://www.readbyqxmd.com/read/28170296/muse-cells-derived-from-dermal-tissues-can-differentiate-into-melanocytes
#9
Ting Tian, Ru-Zhi Zhang, Yu-Hua Yang, Qi Liu, Di Li, Xiao-Ru Pan
The objective of the authors has been to obtain multilineage-differentiating stress-enduring cells (Muse cells) from primary cultures of dermal fibroblasts, identify their pluripotency, and detect their ability to differentiate into melanocytes. The distribution of SSEA-3-positive cells in human scalp skin was assessed by immunohistochemistry, and the distribution of Oct4, Sox2, Nanog, and SSEA-3-positive cells was determined by immunofluorescence staining. The expression levels of Sox2, Oct4, hKlf4, and Nanog mRNAs and proteins in Muse cells were determined by reverse transcription polymerase chain reaction (RT-PCR) analyses and Western blots, respectively...
April 2017: Cellular Reprogramming
https://www.readbyqxmd.com/read/28157763/effect-of-procedural-related-variables-on-melanocyte-keratinocyte-suspension-transplantation-in-nonsegmental-stable-vitiligo-a-clinical-and-immunocytochemical-study
#10
MULTICENTER STUDY
Bakr Mohamed El-Zawahry, Samia Esmat, Dalia Bassiouny, Naglaa Sameh Zaki, Rehab Sobhi, Marwah A Saleh, Dalia Abdel-Halim, Rehab Hegazy, Heba Gawdat, Nesrin Samir, Marwa El-Hawary, Zeinab El Maadawi, Heba Gouda, Mervat Khorshied
BACKGROUND: Melanocyte-keratinocyte suspension (M-K susp) is gaining popularity for vitiligo treatment. Few studies have addressed procedure-related variables. OBJECTIVE: To assess the effect of different M-K susp procedure-related variables on the clinical outcome in stable vitiligo. METHODS: This prospective multicenter comparative study included 40 cases with nonsegmental stable vitiligo. Donor site was either a skin graft in noncultured epidermal cell suspension (NCECS) or hair follicle units in outer root sheath hair follicle suspension (ORSHFS)...
February 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/28105227/milia-may-originate-from-the-outermost-layers-of-the-hair-bulge-of-the-outer-root-sheath-a-case-report
#11
Ichiro Kurokawa, Ayako Kakuno, Airo Tsubura
It has been hypothesized that milia originate from the hair bulge of the outer root sheath. To elucidate the histogenesis of milia, an immunohistochemical study was performed using anti-keratin and anti-filaggrin antibodies to determine the levels of keratin and filaggrin expression. Keratin expression was evaluated using anti-keratin antibodies against K1, K7, K8, K10, K14, K15, K16, K17, K18, K19 and K20. K1 and K10 expression were detected in the suprabasal layers of the more superficial section of the cyst walls, but not in the deeper section of the cyst walls...
December 2016: Oncology Letters
https://www.readbyqxmd.com/read/28099203/evaluation-of-gene-expression-patterns-in-micrografts-demonstrate-induction-of-catagen-like-processes-during-storage
#12
Walter Krugluger, Karl Stiefsohn, Karl Moser, Claudia Moser, Katarina Laciak
BACKGROUND: Alterations of gene expression patterns may contribute to the commonly observed transient reduction of hair shaft elongation in hair restoration surgery. OBJECTIVE: To elucidate the molecular causes, we evaluated changes in gene expression patterns in hair follicle micrografts during storage. MATERIALS AND METHODS: Micrografts with different amounts of adjacent connective tissue (regular, skinny, and chubby) were stored for different periods, and the expression of key genes was determined: dermal papilla (DP): FGF7, alkaline phosphatase (ALP), versican; outer root sheath: Krt15; inner root sheath: Krt 25; cuticula: Krt85; Henle layer: filaggrin; genes related to apoptosis and growth/differentiation: Caspase 3, Ovol1, and Foxo1...
February 2017: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/27904857/t-cadherin-expression-in-the-epidermis-and-adnexal-structures-of-normal-skin
#13
Stanislaw Buechner, Paul Erne, Therese J Resink
BACKGROUND: T-cadherin is an atypical glycosylphosphatidylinositol-anchored member of the cadherin superfamily of adhesion molecules. The role of T-cadherin in biology of the skin is poorly understood. Expression of T-cadherin in basal keratinocytes and dermal blood vessels of the healthy epidermis has been demonstrated, but studies on expression in skin appendages are rare. METHODS: We conducted an immunohistochemical analysis of T-cadherin expression in the epidermis and adnexal structures of normal skin...
September 2016: Dermatopathology (Basel, Switzerland)
https://www.readbyqxmd.com/read/27904182/comparative-study-of-efficacy-of-epidermal-melanocyte-transfer-versus-hair-follicular-melanocyte-transfer-in-stable-vitiligo
#14
Navya Donaparthi, Ajay Chopra
BACKGROUND: Vitiligo surgery has come up a long way from punch skin grafts to epidermal cell suspension and latest to the extracted hair follicle outer root sheath cell suspension (EHFORSCS) transplantation. The progressive development from one technique to the other is always on a quest for the best. In the latest development, EHFORSCS, which is an enriched source of follicular inactive melanocyte (melanocyte stem cells), seems to be a good addition to the prevailing cell-based therapies for vitiligo...
November 2016: Indian Journal of Dermatology
https://www.readbyqxmd.com/read/27733339/expression-and-localization-of-gpr143-in-sheep-skin
#15
Chen Tianzhi, Zhao Bingling, Liu Yu, Zhao Yuanyuan, Wang Haidong, Fan Ruiwen, Wang Pengchao, Dong Changsheng
G-protein coupled receptor143 (GPR143) plays an important role in melanogenesis. In this study, we investigated the expression pattern and localization of GPR143 in skin of sheep with different coat colors and explored the correlation between GPR143 gene and coat color. The mRNA level and protein level of GPR143 in skin of sheep with different coat colors were detected by qRT-PCR and immunoblotting separately while the localization of GPR143 in sheep skin was detected by immunofluorescence assay following optical density analysis...
July 20, 2016: Yi Chuan, Hereditas
https://www.readbyqxmd.com/read/27690000/ocular-albinism-type-1-regulates-melanogenesis-in-mouse-melanocytes
#16
Tianzhi Chen, Haidong Wang, Yu Liu, Bingling Zhao, Yuanyuan Zhao, Ruiwen Fan, Pengchao Wang, Changsheng Dong
To investigate whether ocular albinism type 1 (OA1) is differentially expressed in the skin of mice with different coat colors and to determine its correlation with coat color establishment in mouse, the expression patterns and tissue distribution characterization of OA1 in the skin of mice with different coat colors were qualitatively and quantitatively analyzed by real-time quantitative PCR (qRT-PCR), immunofluorescence staining and Western blot. The qRT-PCR analysis revealed that OA1 mRNA was expressed in all mice skin samples tested, with the highest expression level in brown skin, a moderate expression level in black skin and the lowest expression level in gray skin...
September 27, 2016: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/27683091/linear-trichilemmomas-on-the-ankle-of-a-28-year-old-female
#17
Jessica L Gonzalez, Natalia Plotnikova, Andrew Seymour, Ciaran M Mannion, Richard Danialan, David Rosmarin
Trichilemmomas are benign cutaneous proliferations derived from the outer root sheath of the hair follicle. They most often occur on the head and neck region and show a female predominance. When multiple, they are associated with Cowden syndrome (CS), a rare disorder due to an autosomal dominant germline mutation in PTEN (phosphatase and tensin homolog on chromosome 10), a tumor suppressor gene. Trichilemmomas outside of the head and neck region are rare, and as such, the association with CS is not clear. A 28-year-old healthy female with no significant family history of cancer presented to her dermatologist with multiple erythematous papules on the left anterior ankle, starting at birth...
January 2017: Journal of Cutaneous Pathology
https://www.readbyqxmd.com/read/27611344/daughters-of-the-enamel-organ-development-fate-and-function-of-the-stratum-intermedium-stellate-reticulum-and-outer-enamel-epithelium
#18
Hui Liu, Xiulin Yan, Mirali Pandya, Xianghong Luan, Thomas G H Diekwisch
The tooth enamel organ (EO) is a complex epithelial cell assembly involved in multiple aspects of tooth development, including amelogenesis. The present study focuses on the role of the nonameloblast layers of the EO, the stratum intermedium, the stellate reticulum, and the outer enamel epithelium (OEE). The secretory stage stratum intermedium was distinguished by p63-positive epithelial stem cell marks, highly specific alkaline phosphatase labeling, as well as multiple desmosomes and gap junctions. At the location of the presecretory stage stellate reticulum, the pre-eruption EO prominently featured the papillary layer (PL) as a keratin immunopositive network of epithelial strands between tooth crowns and oral epithelium...
September 9, 2016: Stem Cells and Development
https://www.readbyqxmd.com/read/27601859/a-study-of-noncultured-extracted-hair-follicle-outer-root-sheath-cell-suspension-for-transplantation-in-vitiligo
#19
Aarti N Shah, Ritu K Marfatia, Siddhartha S Saikia
CONTEXT: Vitiligo surgeries have come a long way from tissue grafts to cultured and non cultured cell transplantation. Extracted hair follicle outer root sheath cell transplantation (EHF ORS) suspension is more enriched with melanocyte. In a hair bulb, there is one melanocyte for every five keratinocytes which is much higher than the epidermal melanin unit. AIMS: To analyse the effectiveness of cultured EHF ORS and to perform objective evaluation based on clinical improvement & photographic evidence...
April 2016: International Journal of Trichology
https://www.readbyqxmd.com/read/27587291/localization-of-the-rabies-virus-antigen-in-merkel-cells-in-the-follicle-sinus-complexes-of-muzzle-skins-of-rabid-dogs
#20
Taichi Shimatsu, Harumi Shinozaki, Kazunori Kimitsuki, Nozomi Shiwa, Daria L Manalo, Rodolfo C Perez, Joselito E Dilig, Kentaro Yamada, Hassadin Boonsriroj, Satoshi Inoue, Chun-Ho Park
The direct fluorescent antibody test (dFAT) on fresh brain tissues is the gold standard for rabies virus antigen detection in dogs. However, this method is laborious and holds a high risk of virus exposure for the experimenter. Skin biopsies are useful for the diagnosis of humans and animals. In mammals, the tactile hair, known as the follicle-sinus complex (FSC), is a specialized touch organ that is abundant in the muzzle skin. Each tactile hair is equipped with more than 2,000 sensory nerve endings. Therefore, this organ is expected to serve as an alternative postmortem diagnostic material...
November 2016: Journal of Virological Methods
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