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Outer root sheath

Tianzhi Chen, Bingling Zhao, Yu Liu, Ruiwei Wang, Yujing Yang, Linpei Yang, Changsheng Dong
BACKGROUND: Although the impact of the microphthalamia-associated transcription factor (Mitf) signaling pathway on melanocytes progression has been extensively studied, the specific molecular mechanisms behind MITF-M-enhanced melanin production in melanocytes still need to be clarified. METHODS: In this study, we analyzed the levels of Mitf-M in skin tissues of different coat mice in order to further reveal the relationship between Mitf-M and skin pigmentation. To address the function of Mitf-M on melanogenesis, we have used an overexpression system and combined morphological and biochemical methods to investigate its localization in different coat color mice and pigmentation-related genes' expression in mouse melanocytes...
February 19, 2018: Journal of Dermatological Science
Mustafa Kürşat Evrenos, Hakan Kerem, Peyker Temiz, Aylin Türel Ermertcan, Levent Yoleri
OBJECTIVES: To emphasize different clinical features of tumor that can be misdiagnosed clinically. METHODS: A total of 8 cases operated between September 2009 and 2016 at the Celal Bayar University, Faculty of Medicine were included in the study. Patients' clinicopathological features, type of surgery and follow up information were evaluated. Results: Six patients were male. The average age was 75.50. The lesions were located on the head and neck, and chest wall...
February 2018: Saudi Medical Journal
Amélie Nicolau, Karin Lemberger, Marion Mosca, Antoine Leclerc, Alexis Lécu, Didier Pin
BACKGROUND: Captive Andean bears (Tremarctos ornatus) develop a distinct alopecic syndrome of unknown aetiology. HYPOTHESIS/OBJECTIVES: To describe the histological features of healthy Andean bear skin, to define the clinical and histopathological features of Andean bears with signs of alopecia, and to propose an aetiopathogenesis. ANIMALS: Eighteen healthy Andean bears housed in 12 European zoos and 13 Andean bears with mild to severe alopecia housed in nine European zoos...
January 31, 2018: Veterinary Dermatology
Nai Rile, Zhihong Liu, Lixia Gao, Jingkai Qi, Meng Zhao, Yuchun Xie, Rui Su, Yanjun Zhang, Ruijun Wang, Jie Li, Hongmei Xiao, Jinquan Li
BACKGROUND: The growth of Inner Mongolian Cashmere goat skin hair follicle exhibits a periodic growth pattern. The hair growth cycle is distinguished as telogen, anagen, and catagen stages. The role of vimentin in the growth process of hair follicles is evident. To elucidate the mechanism underlying the vimentin activity in the growth cycle of hair follicles, transcriptome sequencing and liquid chromatography-tandem mass spectrometry were used to obtain the nucleic acid and amino acid sequences of VIIM gene and vimentin...
January 10, 2018: BMC Genomics
Ilhame Naciri, Baderddine Hassam
Proliferating trichilemmal tumor (PTT), still referred to as proliferative trichilemmal cyst (TC), is an uncommon malignant adnexal tumor originating from the cells of the outer root sheath of the hair follicle or, more often, from a trichilemmal cyst, following multiple trauma and/or recurrent inflammation. We report the case of a 64-year old female patient, with no particular previous history, presenting with tumor of the scalp gradually evolving over 18 months. Clinical examination showed a painless, firm, adhering ulcero-budding tumor mass measuring 12 cm along its longer axis at the level of the vertex...
2017: Pan African Medical Journal
Liang-Li Song, Yan Cui, Si-Jiu Yu, Peng-Gang Liu, Jun Liu, Xue Yang, Jun-Feng He, Qian Zhang
Bone morphogenetic protein 2 (BMP2), BMP receptor-IA (BMPR-IA), and the BMP2 antagonist Noggin are important proteins involved in regulating the hair follicle (HF) cycle in skin. In order to explore the expression profiles of BMP2, BMPR-IA, and Noggin in the HF cycle of yak skin, we collected adult yak skin in the telogen, proanagen, and midanagen phases of HFs and evaluated gene and protein expression by real-time quantitative polymerase chain reaction (qRT-PCR), western blotting, and immunohistochemistry...
November 22, 2017: General and Comparative Endocrinology
Yutaro Yamamoto, Takamitsu Makino, Hideo Kudo, Hironobu Ihn, Yasuko Murakami, Senya Matsufuji, Kunio Fujiwara, Masashi Shin
No expression and distribution patterns of polyamines (PAs), spermine, spermidine, and their precursor putrescine in mammalian hair follicle are available, although polyamines are known to correlate well with hair growth and epidermal tumor genesis. Immunohistochemistry (IHC) using our original two monoclonal antibodies (mAbs) ASPM-29 specific for spermine or spermidine, and APUT-32 specific for putrescine allowed us to detect immunoreactivity for polyamines in hair follicles from normal adult rats. A wide range of immunoreactivity for the total spermine and spermidine was observed in the compartments of hair follicle: The highest degree of immunoreactivity for polyamines was observed in the matrix, in the Huxley's layer, in the deeper Henle's layer, and in the cuticle of the inner root sheath/the hair cuticle, while moderate immunoreactivity existed in the lower-to-mid cortex and the companion layer, followed by lower immunoreactivity in the outer root sheath, including the bulge region and in the deeper medulla, in which the immunoreactivity was also evident in their nuclei...
November 21, 2017: Histochemistry and Cell Biology
Jiqing Wang, Ping Yu, Haifang Wang, Yanyu He
This experiment was conducted to identify periodic changes in secondary follicles (SFs) over one year of extensive (grazed) and intensive (housed and fed supplement) farming of Longdong cashmere goats. One-year old goats (n = 24, 12 extensively fed and 12 intensively fed) were studied. The diameter, depth, density, activity, and ultra-structural features of SFs were assessed using light microscopy and transmission electron microscopy. HOXC13 and HSP27 expression were studied using immunohistochemistry and immunofluorescence method...
November 17, 2017: Anatomical Record: Advances in Integrative Anatomy and Evolutionary Biology
Talveen S Purba, Michael Peake, Bessam Farjo, Nilofer Farjo, Ranjit K Bhogal, Gail Jenkins, Ralf Paus
Human scalp hair follicles (hHF) harbour several epithelial stem (eHFSC) and progenitor cell sub-populations organised into spatially distinct niches. However, the constitutive cell cycle activity of these niches remains to be characterized in situ. Therefore, the current study has studied these characteristics of keratin 15+ (K15), CD200+ or CD34+ cells within anagen VI hHFs by immunohistomorphometry, using Ki-67 and 5-ethynyl-2'-deoxyuridine (EdU). We quantitatively demonstrate in situ the relative cell cycle inactivity of the CD200+/K15+ bulge compared to other non-bulge CD34+ and K15+ progenitor compartments and found that in each recognized eHFSC/progenitor niche, proliferation associates negatively with eHFSC-marker expression...
November 9, 2017: Scientific Reports
Lysann Kroschwald, Meinolf Suttorp, Josephine Tabea Tauer, Nick Zimmermann, Claudia Günther, Andrea Bauer
Prolonged treatment with tyrosine kinase inhibitors (TKI) including imatinib (IMA) or nilotinib (NIL), induces severe disturbances of bone metabolism in patients with chronic myeloid leukaemia. As vitamin D3 (VD3) is involved in the complex cycle of bone remodelling, the present study investigated in vitro, the influence of IMA and NIL on VD3 metabolism i) in HaCaT cells and ii) in cultured outer root sheath keratinocytes (ORS‑KC) from hair follicles of IMA treated children. Cells were incubated in the presence of IMA or NIL...
January 2018: Molecular Medicine Reports
Bum-Ho Bin, Jinhyuk Bhin, Mikiro Takaishi, Koh-Ei Toyoshima, Saeko Kawamata, Kana Ito, Takafumi Hara, Takashi Watanabe, Tarou Irié, Teruhisa Takagishi, Su-Hyon Lee, Haeng-Sun Jung, Sangchul Rho, Juyeon Seo, Dong-Hwa Choi, Daehee Hwang, Haruhiko Koseki, Osamu Ohara, Shigetoshi Sano, Takashi Tsuji, Kenji Mishima, Toshiyuki Fukada
Skin tissues, in particular the epidermis, are severely affected by zinc deficiency. However, the zinc-mediated mechanisms that maintain the cells that form the epidermis have not been established. Here, we report that the zinc transporter ZIP10 is highly expressed in the outer root sheath of hair follicles and plays critical roles in epidermal development. We found that ZIP10 marked epidermal progenitor cell subsets and that ablating Zip10 caused significant epidermal hypoplasia accompanied by down-regulation of the transactivation of p63, a master regulator of epidermal progenitor cell proliferation and differentiation...
November 14, 2017: Proceedings of the National Academy of Sciences of the United States of America
Haihua Zhang, Weixiao Nan, Xingchao Song, Shiyong Wang, Huazhe Si, Guangyu Li
AIMS: Outer root sheath (ORS) is a highly proliferative component of a hair follicle. This study is performed to investigate whether hypoxia-induced elevation of hypoxia-inducible factor (HIF)-1α, a transcriptional activator, contributes to the outgrowth of ORS cells in vitro. MAIN METHODS: Hair follicles with intact ORS collected from 4-month old male American minks were cultured in normoxic or hypoxic condition (3% oxygen) for 7days. Primary ORS cells isolated from the mink hair follicles were exposed to hypoxia for 12, 24 or 48h, and their proliferation was analyzed with immunofluorescence assay using anti-proliferating cell nuclear antigen (PCNA) antibody...
December 15, 2017: Life Sciences
Reena Rai, Manu V Harikumar
BACKGROUND: Pemphigus is a chronic autoimmune bullous disorder characterized by autoantibodies directed against desmoglein 3 and/or 1. Demonstration of intercellular deposition of IgG on the cell surface of keratinocytes by direct immunofluorescence (DIF) of the skin is the gold standard in the diagnosis of pemphigus. Recently, DIF of plucked hair demonstrating intercellular deposition of IgG in the outer root sheath (ORS) has shown to be useful. OBJECTIVE: To compare the DIF of plucked hair and skin for the evaluation of immunological remission in pemphigus vulgaris patients in clinical remission...
September 2017: Indian Dermatology Online Journal
Pawan Kumar, Neetu Bhari, Manoj K Tembhre, Sujata Mohanty, Sudheer Arava, Vinod K Sharma, Somesh Gupta
BACKGROUND: Noncultured, extracted follicular outer root sheath suspension (NC-EHF-ORS-CS) is a recently introduced technique for the treatment of stable vitiligo. OBJECTIVE: To study the clinical efficacy of this technique and to determine the viability and cell composition of the suspension. METHODS: Twenty-five patients with stable vitiligo were included in this prospective study. Fifty follicles were extracted from occipital scalp and were incubated with trypsin-ethylenediaminetetraacetic acid to separate outer root sheath cells...
October 3, 2017: International Journal of Dermatology
Angel Fernandez Flores, Adrian Varela-Vazquez, Maria D Mayan, Eduardo Fonseca
BACKGROUND: Gap junctions form communication compartments between cells. These channels assemble from connexin subunits. OBJECTIVE: To investigate the immunoexpression of connexin 43 (Cx43) in adult human hair follicles. METHODS: Cases were retrospectively obtained from our archives. RESULTS: We identified immunoexpression of Cx43 in the matrix, the papilla, the outer root sheath, the bulge, the medulla, the cortex, the shaft and the secretory part of the sebaceous gland...
January 2018: Journal of Cutaneous Pathology
Jung E Kim, Young J Woo, Ki M Sohn, Kwan H Jeong, Hoon Kang
BACKGROUND: Outer root sheath cells (ORSCs) play important roles in maintaining hair follicle structure and provide support for the bulge area. The hair growth promoting effects of photobiomodulation therapy (PBMT) have been reported, but the mechanisms for this in human ORCs (hORSCs) have rarely been studied. OBJECTIVE: The aim of this study was to investigate the effect of various wavelengths of light-emitting diode (LED) irradiation on human ORSCs (hORSCs). METHODS: LED irradiation effects on hORSC proliferation and migration were examined with MTT assay, BrdU incorporation assay and migration assays...
September 25, 2017: Lasers in Surgery and Medicine
Wen-Yen Huang, Shih-Fan Lai, Hsien-Yi Chiu, Michael Chang, Maksim V Plikus, Chih-Chieh Chan, You-Tzung Chen, Po-Nien Tsao, Tsung-Lin Yang, Hsuan-Shu Lee, Peter Chi, Sung-Jan Lin
Genotoxicity-induced hair loss from chemotherapy and radiotherapy is often encountered in cancer treatment, and there is a lack of effective treatment. In growing hair follicles (HF), quiescent stem cells (SC) are maintained in the bulge region, and hair bulbs at the base contain rapidly dividing, yet genotoxicity-sensitive transit-amplifying cells (TAC) that maintain hair growth. How genotoxicity-induced HF injury is repaired remains unclear. We report here that HFs mobilize ectopic progenitors from distinct TAC compartments for regeneration in adaptation to the severity of dystrophy induced by ionizing radiation (IR)...
November 15, 2017: Cancer Research
Christopher Jones, Mark Twoon, Weiguang Ho, Mark Portelli, Bernard F Robertson, William Anderson
Pilomatrix carcinoma is a rare, locally aggressive tumor with a tendency to recur. Distant metastases have been reported, with pulmonary lesions being the most frequent manifestation. Similar to pilomatrixoma, pilomatrix carcinoma typically presents as a nontender, firm dermal swelling and is found most commonly in the head and neck region. Although pilomatrixomas and pilomatrix carcinoma are well-recognized lesions, clinically they are frequently misdiagnosed as other skin conditions. By reviewing the literature over the past 10 years, the aims of this review are to analyze the cause, clinical presentation, histopathologic features, management and outcomes of pilomatrix carcinoma amongst children and adults...
January 2018: Journal of Cutaneous Pathology
Marie Schneider, Andrea Lohrenz, Michael Cross, Michael C Hacker, Jan C Simon, Vuk Savkovic
Formulating clinically relevant melanocyte cultivation media that maintain the balance between proliferation and maturation to functional melanocytes is a major experimental and regulatory challenge. Within the translation of human melanocytes from the outer root sheath of human hair follicle (HUMORS), we developed a melanocyte medium free of chemical mitogens, chemical melanogenesis enhancers and bovine products, enabling proliferation as well as melanotic differentiation. The formulation involved the replacement of bovine pituitary extract (BPE) and bovine serum (FBS) with human serum (HS) combined with ascorbic acid, CaCl2, epinephrine, L glutamine, insulin and fibroblast growth factor...
August 31, 2017: Experimental Dermatology
Yonghee Lee, Su Na Kim, Yong Deog Hong, Byung Cheol Park, Yongjoo Na
It is well known that Panax ginseng (PG) has various pharmacological effects such as anti-aging and anti-inflammation. In a previous study, the authors identified that PG extract induced hair growth by means of a mechanism similar to that of minoxidil. In the present study, the inhibitory effect of PG extract on Dickkopf-1 (DKK-1)-induced catagen-like changes in hair follicles (HFs) was investigated in addition to the underlying mechanism of action. The effects of PG extract on cell proliferation, anti-apoptotic effect, and hair growth were observed using cultured outer root sheath (ORS) keratinocytes and human HFs with or without DKK-1 treatment...
October 2017: International Journal of Molecular Medicine
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