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N-Acetyl Cysteine Antioxidant

Tamás Gáll, Dávid Pethő, Annamária Nagy, Zoltán Hendrik, Gábor Méhes, László Potor, Magnus Gram, Bo Åkerström, Ann Smith, Péter Nagy, György Balla, József Balla
Accumulation of damaged or misfolded proteins resulted from oxidative protein modification induces endoplasmic reticulum (ER) stress by activating the pathways of unfolded protein response. In pathologic hemolytic conditions, extracellular free hemoglobin is submitted to rapid oxidation causing heme release. Resident cells of atherosclerotic lesions, after intraplaque hemorrhage, are exposed to heme leading to oxidative injury. Therefore, we raised the question whether heme can also provoke ER stress. Smooth muscle cells are one of the key players of atherogenesis; thus, human aortic smooth muscle cells (HAoSMCs) were selected as a model cell to reveal the possible link between heme and ER stress...
2018: Frontiers in Physiology
Sandeep B Subramanya, Balaji Venkataraman, Mohamed Fizur Nagoor Meeran, Sameer N Goyal, Chandragouda R Patil, Shreesh Ojha
Acetaminophen (APAP), which is also known as paracetamol or N -acetyl- p -aminophenol is a safe and potent drug for fever, pain and inflammation when used at its normal therapeutic doses. It is available as over-the-counter drug and used by all the age groups. The overdose results in acute liver failure that often requires liver transplantation. Current clinical therapy for APAP-induced liver toxicity is the administration of N -acetyl-cysteine (NAC), a sulphydryl compound an approved drug which acts by replenishing cellular glutathione (GSH) stores in the liver...
November 28, 2018: International Journal of Molecular Sciences
Elizabeth Hernandez Marin, Hana Paek, Mei Li, Yesung Ban, Marie Katie Karaga, Rangaiah Shashidharamurthy, Xinyu Wang
Caffeic acid phenethyl ester (CAPE) is a phenolic compound initially identified in bee glue. CAPE is reported to exhibit antitumor activity in many cancer models. However, the effect of CAPE on multiple myeloma (MM) is not well studied. We investigated the anti-myeloma effect of CAPE, and the data showed that CAPE inhibited the growth of human MM cells in a dose (1 ~ 30 μM) and time (24 ~72 h) dependent manner without altering the viability of normal human peripheral blood B cells. Stress and toxicity pathway analysis demonstrated that CAPE, in a dose- and time-related fashion, induced the expression of apoptotic and oxidative stress-response genes including growth arrest and DNA-damage inducible, alpha and gamma (GADD45A and GADD45G) and heme oxygenase-1...
November 22, 2018: Investigational New Drugs
Tahir Ali, Iram Mushtaq, Sonia Maryam, Anam Farhan, Kiran Saba, Muhammad Ishtiaq Jan, Aneesa Sultan, Mariam Anees, Burcu Duygu, Sadia Hamera, Sobia Tabassum, Qamar Javed, Paula A da Costa Martins, Iram Murtaza
Early and specific diagnosis of oxidative stress linked diseases as cardiac heart diseases remains a major dilemma for researchers and clinicians. MicroRNAs may serve as a better tool for specific early diagnostics and propose their utilization in future molecular medicines. We aimed to measure the microRNAs expressions in oxidative stress linked cardiac hypertrophic condition induced through stimulants as Endothelin and Isoproterenol. Cardiac hypertrophic animal models were confirmed by BNP, GATA4 expression, histological assays, and increased cell surface area...
November 12, 2018: Archives of Biochemistry and Biophysics
I İpek Boşgelmez, Gülin Güvendik
Hexavalent chromium[Cr(VI)] compounds may induce toxic effects, possibly via reactive intermediates and radicals formed during Cr(VI) reduction. In this study, we probed the possible effects of N-acetyl-L-cysteine (NAC) and taurine pre- or post-treatments on Cr(VI)-induced changes in lipid peroxidation and nonprotein thiols (NPSH) in mice heart, lung, spleen, and testis tissues. The mice were randomly assigned to six groups, consisting of control, Cr(VI)-exposed (20 mg Cr/kg, intraperitoneal ,ip), NAC (200 mg/kg, ip) as pre-treatment and post-treatment, and taurine (1 g/kg, ip) pre-treatment and post-treatment groups...
November 12, 2018: Biological Trace Element Research
Yiqiang Hu, Zengwu Shao, Xianyi Cai, Yunlu Liu, Min Shen, Yingtao Yao, Tian Yuan, Wentian Wang, Fan Ding, Liming Xiong
STUDY DESIGN: Experimental study. OBJECTIVE: The purposes of this study were to evaluate whether advanced glycation end-products (AGEs) induce annulus fibrosus (AF) cell apoptosis and further to explore the mechanism by which this process occurs. SUMMARY OF BACKGROUND DATA: Recent studies revealed that AGEs accumulation is considered an important factor in diabetic intervertebral disc (IVD) degeneration. However, the effect of AGEs on intervertebral disc remains unclear...
November 6, 2018: Spine
Ruoyun Gao, Tingting Li, Brandon Tan, Suzane Ramos da Silva, Jae U Jung, Pinghui Feng, Shou-Jiang Gao
Kaposi's sarcoma-associated herpesvirus (KSHV) has latent and lytic replication phases, both of which contribute to the development of KSHV-induced malignancies. Among numerous factors identified to regulate KSHV life cycle, oxidative stress, caused by imbalanced clearing and production of reactive oxygen species (ROS), has been shown to robustly disrupt KSHV latency and induce viral lytic replication. In this study, we identify an important role of antioxidant defense factor forkhead box protein O1 (FoxO1) in KSHV lifecycle...
November 7, 2018: Journal of Virology
Basma G Eid, Alaa T Abu-Sharib, Hany M El-Bassossy, Khadijah Balamash, Sergey V Smirnov
Advanced glycation end-products (AGEs) play a pivotal role in macro- and micro-vascular diabetic complications. We investigated the mechanism by which methylglyoxal (an endogenous generator of AGEs) affects vascular contractility using the isolated artery technique. Contractile responses to vasoconstrictors phenylephrine (PE), angiotensin II (Ang II), vasopressin (VP) and KCl were measured in the isolated rat aorta following one-our exposure to methylglyoxal (50-200 μM). The perfused rat kidney was employed to confirm the effect of methylglyoxal on microvessels...
November 4, 2018: Biochemical and Biophysical Research Communications
Ali Seidkhani-Nahal, Abdolamir Allameh, Masoud Soleimani
This study was carried out to examine the role of intracellular albumin in the modulation of oxidative damage induced by glutathione modifiers in HepG2 cells. Also, the relationship of albumin synthesis with oxidative stress factors including antioxidants was studied. HepG2 cell culture was supplemented with glutathione modifiers; L-Buthionine-sulfoximine (BSO; 0.1 and 1.0 mM) or N-acetyl cysteine (NAC; 1 and 10 mM) and the cell viability and changes in reduced glutathione (GSH), oxidized glutathione (GSSG), reactive oxygen species (ROS), catalase, and superoxide dismutase were measured...
November 7, 2018: Biotechnology and Applied Biochemistry
Maqusood Ahamed, Mohd Javed Akhtar, M A Majeed Khan, Salman A Alrokayan, Hisham A Alhadlaq
Bismuth oxide nanoparticles (Bi2 O3 NPs) have shown great potential for several applications including cosmetics and biomedicine. However, there is paucity of research on toxicity of Bi2 O3 NPs. In this study, we first examined dose-dependent cytotoxicity and apoptosis response of Bi2 O3 NPs in human breast cancer (MCF-7) cells. We further explored the potential mechanisms of cytotoxicity of Bi2 O3 NPs through oxidative stress. Physicochemical study demonstrated that Bi2 O3 NPs have crystalline structure and spherical shape with mean size of 97 nm...
February 2019: Chemosphere
Fan Yang, Ruonan Pei, Zhuowei Zhang, Jianzhao Liao, Wenlan Yu, Na Qiao, Qingyue Han, Ying Li, Lianmei Hu, Jianying Guo, Jiaqiang Pan, Zhaoxin Tang
The aim of this study was to investigate the effects of excessive copper (Cu)-induced cytotoxicity on oxidative stress and mitochondrial apoptosis in chicken hepatocytes. Chicken hepatocytes were cultured in medium in the absence and presence of copper sulfate (CuSO4 ) (10, 50, 100 μM), in N-acetyl-L-cysteine (NAC) (1 mM), and the combination of CuSO4 and NAC for 24 h. Morphologic observation and function, reactive oxygen species (ROS) level, antioxidant indices, nitric oxide (NO) content, mitochondrial membrane potential (MMP), and apoptosis-related mRNA and protein levels were determined...
October 30, 2018: Toxicology in Vitro: An International Journal Published in Association with BIBRA
Hui Wang, Xiaolin Xing, Jipeng Wang, Bo Pang, Ming Liu, Jessie Larios-Valencia, Tao Liu, Ge Liu, Saijun Xie, Guijuan Hao, Zhi Liu, Biao Kan, Jun Zhu
Bacterial pathogens are highly adaptable organisms, a quality that enables them to overcome changing hostile environments. For example, Vibrio cholerae, the causative agent of cholera, is able to colonize host small intestines and combat host-produced reactive oxygen species (ROS) during infection. To dissect the molecular mechanisms utilized by V. cholerae to overcome ROS in vivo, we performed a whole-genome transposon sequencing analysis (Tn-seq) by comparing gene requirements for colonization using adult mice with and without the treatment of the antioxidant, N-acetyl cysteine...
October 2018: PLoS Pathogens
Pengpu Wang, Rongrong Ji, Junfu Ji, Fang Chen
Blueberry anthocyanins extract (BAE) is widely used natural antioxidant. The inhibitory effect of BAE (175 mg/kg bw/day) pretreatment on glycidamide (GA) metabolism following acrylamide (AA) (35 mg/kg bw/day) gavage for consecutive 7 days and 14 days in SD rat model were investigated in this work. AA, GA and its metabolites in urine, blood and 3 tissues were quantified using UPLC-MS/MS, respectively. The results showed pretreatment with BAE could significantly block the epoxidation of AA to GA. Compared with the control, BAE pretreatment significantly decreased the amount of GA and its mercapturic acid metabolite N-acetyl-S-(3-amino-2-hydroxy-3-oxopropyl)-cysteine (GAMA3) in rats urine (p < 0...
February 15, 2019: Food Chemistry
Maiara de S Oliveira, Marília I F Barbosa, Thiago Belarmino de Souza, Diogo R M Moreira, Felipe Terra Martins, Wilmer Villarreal, Rafael P Machado, Antônio Carlos Doriguetto, Milena B P Soares, Daniel P Bezerra
Piplartine (piperlongumine) is a plant-derived compound found in some Piper species that became a novel potential antineoplastic agent. In the present study, we synthesized a novel platinum complex containing a piplartine derivative cis-[PtCl(PIP-OH)(PPh3 )2 ]PF6 (where, PIP-OH = piplartine demethylated derivative; and PPh3 = triphenylphosphine) with enhanced cytotoxicity in different cancer cells, and investigated its apoptotic action in human promyelocytic leukemia HL-60 cells. The structure of PIP-OH ligand was characterized by X-ray crystallographic analysis and the resulting platinum complex was characterized by infrared, molar conductance measurements, elemental analysis and NMR experiments...
October 12, 2018: Redox Biology
Rohan Moniruzzaman, Mati Ur Rehman, Qing-Li Zhao, Paras Jawaid, Yohei Mitsuhashi, Shuichi Imaue, Kumiko Fujiwara, Ryohei Ogawa, Kei Tomihara, Jun-Ichi Saitoh, Kyo Noguchi, Takashi Kondo, Makoto Noguchi
Sulfasalazine (SSZ) is a well-known anti-inflammatory drug and also an inhibitor of the cystine-glutamate antiporter that is known to reduce intracellular glutathione (GSH) level and increase cellular oxidative stress, indicating its anti-tumor potential. However, the combination of SSZ with other physical modalities remains unexplored. Here, the effects of SSZ on cold atmospheric helium plasma (He-CAP), which produces approximately 24 x higher concentration of hydroxyl radicals (. OH) compared to X-irradiation (IR) in aqueous solution, and on IR-induced apoptosis in human leukemia Molt-4 cells were studied to elucidate the mechanism of apoptosis enhancement...
December 2018: Free Radical Biology & Medicine
Debraj Gangopadhyay, Moumita Das, Keshav Kumar Singh, Poornima Sharma, Ranjan K Singh, Poonam Tandon
The important cyclization reaction of antioxidant drug N-acetyl-l-cysteine (NAC) has been monitored in vitro at basic pH with the help of time series Raman spectroscopy. The thiazoline ring formation of NAC at acidic pH is a well-known reaction and has been studied extensively. However, the formation of a thiazolidine ring from NAC at basic pH has not been investigated precisely till date. The effect of basicity of the medium on the rate of cyclization has been investigated by studying the reaction at five different basic pH values...
November 15, 2018: Journal of Physical Chemistry. B
Sanju Kumari, Abhishek Mukherjee, Chinmay K Mukhopadhyay
Recent literature suggested an important function of native amyloid precursor protein (APP) as amine oxidase implicating in protection of brain cells from catecholamine-induced toxicity. However, any role of catecholamines on regulation of native APP has not been explored. Here we report that dopamine (DA), one of the most prominent catecholamine neurotransmitters in brain, down-modulates native APP protein in several neuronal cell types. Using SH-SY5Y cells as model, we detected no alteration of transcript expression and unaffected translation suggested that DA might induce APP degradation...
October 22, 2018: Molecular and Cellular Biochemistry
Shen Tang, Cailing Lu, Laiming Mo, Xinhang Wang, Ziwei Liang, Fu Qin, Yinpin Liu, Yuyang Liu, Haiyan Huang, Yue Huang, Haiqing Cai, Deqiang Xiao, Songchao Guo, Yiqiang Ouyang, Bin Sun, Xiyi Li
AIMS: Protein phosphatase methylesterase-1 (PME-1) is a serine hydrolase that catalyzes protein phosphatase 2A (PP2A) demethylation and negatively regulates its activity. PME-1 is compartmentalized within cells to precisely control the demethylation of PP2A. This study investigated the localization of PME-1 in human fibroblast cells (HDF) under oxidative stress. MAIN METHODS: Alkaline demethylation and peptide competition assays were applied to detect the methylation sensitivity of anti-PP2Ac...
October 16, 2018: Life Sciences
Trong Thuan Ung, Thi Thinh Nguyen, Sen Lian, Shinan Li, Yong Xia, Nam Ho Kim, Young Do Jung
Interleukin-6 (IL-6), a pleiotropic cytokine, plays a key role in endothelial injury and atherosclerosis. In this study, we investigated the effects of nicotine, a major psychoactive compound in cigarette smoke, on IL-6 expression and EA.hy926 endothelial cell invasion. Nicotine stimulated IL-6 expression via the activator protein 1 (AP-1) transcription factor. Pharmacological inhibition and mutagenesis studies indicated that p38 mitogen-activated protein kinase (MAPK) mediated the IL-6-induced upregulation of nicotine in EA...
October 14, 2018: Journal of Cellular Biochemistry
Wen-Bo Zhou, Xin-Xin Zhang, Yun Cai, Wu Sun, Hao Li
Tamoxifen (TMX) is an antiestrogen drug that is used in the treatment and prevention of all stages of estrogen-dependent breast cancer. Adverse effects of TMX include hepatotoxicity. In this study, we investigated the therapeutic effects of osthole, isolated from medicinal plants especially Fructus Cnidii, on TMX-induced acute liver injury in mice. Mice were injected with osthole (100 mg/kg, ip) or vehicle, followed by TMX (90 mg/kg, ip) 24 h later. We showed that a single injection of TMX-induced liver injury and oxidative stress...
October 12, 2018: Acta Pharmacologica Sinica
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