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https://www.readbyqxmd.com/read/30310400/the-use-of-stem-cells-in-ischemic-heart-disease-treatment
#1
REVIEW
Radoslaw Litwinowicz, Bogusław Kapelak, Jerzy Sadowski, Anna Kędziora, Krzysztof Bartus
Ischemic heart disease is a major cause of death and disabilities worldwide. Unfortunately, not all patients are suitable for direct revascularization. Cell-based therapies may be alternative options because of their potential to promote neovascularisation and endothelial repair, improving myocardial perfusion. The success of cell-based therapies depends on the type of implanted stem cells, delivery method and underlying disease. Several different cell populations including bone marrow-derived mononuclear cells (MNCs), mesenchymal stromal cells (MSCs), CD34+, CD133+, endothelial progenitor cells, adipose-derived mesenchymal stromal cells (ASCs) and stem cells from placenta and umbilical cord have been investigated...
September 2018: Kardiochirurgia i Torakochirurgia Polska, Polish Journal of Cardio-Thoracic Surgery
https://www.readbyqxmd.com/read/30299207/human-amniotic-membrane-as-differentiating-matrix-for-in-vitro-chondrogenesis
#2
Nadia Naseer, Saliha Bashir, Noreen Latief, Farzana Latif, Shaheen N Khan, Sheikh Riazuddin
AIM: The aim of the present study is to use human amniotic membrane (HAM) for in vitro chondrogenesis of placenta-derived mesenchymal stem cells (MSCs) and umbilical cord-derived MSCs. MATERIALS & METHODS: MSCs from the placenta and umbilical cord were isolated, characterized by immunophenotyping and after analyzing their rate of proliferation, cytotoxicity and viability, chondrogenesis was performed on plastic adherent surface and on HAM. RESULTS: Successfully isolated and characterized placenta-derived MSCs and umbilical cord-derived MSCs revealed positive expression of MSCs markers CD90, CD73, CD105 and CD49d, while they were negative for CD45...
October 2018: Regenerative Medicine
https://www.readbyqxmd.com/read/30276164/potential-use-of-amniotic-membrane-derived-scaffold-for-cerebrospinal-fluid-applications
#3
Fereshteh Dorazehi, Mohammad Nabiuni, Hanieh Jalali
Scaffolds derived from decellularized tissues provide a natural microenvironment for cell culture. Embryonic cerebrospinal fluid (e-CSF) contains factors which play vital roles in the development of the nervous system. This research was aimed to survey the effect of Wistar rat e-CSF on neural differentiation of bone marrow derived mesenchymal stem cells (BM-MSCs) cultured on the human amniotic membrane (AM). BM-MSCs were collected from femurs and tibias, and were cultured in Dulbecco's Modified Eagle's Medium...
2018: International Journal of Molecular and Cellular Medicine
https://www.readbyqxmd.com/read/30272581/regulatory-t-cells-in-embryo-implantation-and-the-immune-response-to-pregnancy
#4
REVIEW
Sarah A Robertson, Alison S Care, Lachlan M Moldenhauer
At implantation, the embryo expresses paternally derived alloantigens and evokes inflammation that can threaten reproductive success. To ensure a robust placenta and sustainable pregnancy, an active state of maternal immune tolerance mediated by CD4+ regulatory T cells (Tregs) is essential. Tregs operate to inhibit effector immunity, contain inflammation, and support maternal vascular adaptations, thereby facilitating trophoblast invasion and placental access to the maternal blood supply. Insufficient Treg numbers or inadequate functional competence are implicated in idiopathic infertility and recurrent miscarriage as well as later-onset pregnancy complications stemming from placental insufficiency, including preeclampsia and fetal growth restriction...
October 1, 2018: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/30257012/placental-formation-in-early-pregnancy-how-is-the-centre-of-the-placenta-made
#5
Anna L Boss, Lawrence W Chamley, Joanna L James
BACKGROUND: Correct development of the placenta is critical to establishing pregnancy and inadequate placentation leads to implantation failure and miscarriage, as well as later gestation pregnancy disorders. Much attention has been focused on the placental trophoblasts and it is clear that the trophoblast lineages arise from the trophectoderm of the blastocyst. In contrast, the cells of the placental mesenchyme are thought to arise from the inner cell mass, but the details of this process are limited...
September 19, 2018: Human Reproduction Update
https://www.readbyqxmd.com/read/30253241/recombinant-pregnancy-specific-glycoprotein-1-has-a-protective-role-in-a-murine-model-of-acute-graft-versus-host-disease
#6
Karlie Jones, Sarah Bryant, Jian Luo, Patricia Kiesler, Sherry Koontz, James Warren, Harry Malech, Elizabeth Kang, Gabriela Dveksler
Acute Graft versus host disease (aGvHD) is an immune mediated reaction that can occur after hematopoietic stem cell transplantation in which donor T-cells recognize the host antigens as foreign, destroying host tissues. Establishment of a tolerogenic immune environment while preserving immune response to infectious agents is required for successful bone marrow transplantation. Pregnancy specific glycoprotein 1 (PSG1) is secreted by the human placenta into the maternal circulation throughout pregnancy. PSG1 likely plays a role in maintaining immunotolerance to prevent rejection of the fetus by the maternal immune system...
September 22, 2018: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/30241570/human-chorionic-villous-mesenchymal-stem-stromal-cells-protect-endothelial-cells-from-injury-induced-by-high-level-of-glucose
#7
Y S Basmaeil, A M Al Subayyil, T Khatlani, E Bahattab, M Al-Alwan, F M Abomaray, B Kalionis, M A Alshabibi, A S AlAskar, M H Abumaree
BACKGROUND: Mesenchymal stem/stromal cells derived from chorionic villi of human term placentae (pMSCs) protect human endothelial cells from injury induced by hydrogen peroxide (H2 O2 ). In diabetes, elevated levels of glucose (hyperglycaemia) induce H2 O2 production, which causes the endothelial dysfunction that underlies the enhanced immune responses and adverse complications associated with diabetes, which leads to thrombosis and atherosclerosis. In this study, we examined the ability of pMSCs to protect endothelial cell functions from the negative impact of high level of glucose...
September 21, 2018: Stem Cell Research & Therapy
https://www.readbyqxmd.com/read/30238990/teeth-derived-stem-cells-a-source-for-cell-therapy
#8
REVIEW
Ghazaleh Baniebrahimi, Razieh Khanmohammadi, Fatemeh Mir
Cell therapy is one of the important therapeutic approaches in the treatment of many diseases such as cancer, degenerative diseases, and cardiovascular diseases. Among various cell types, which could be used as cell therapies, stem cell therapy has emerged as powerful tools in the treatment of several diseases. Multipotent stem cells are one of the main classes of stem cells that could originate from different parts of the body such as bone marrow, adipose, placenta, and tooth. Among several types of multipotent stem cells, tooth-derived stem cells (TDSCs) are associated with special properties such as accessible, easy isolation, and low invasive, which have introduced them as a good source for using in the treatment of several diseases such as neural injuries, liver fibrosis, and Cohrn's disease...
September 21, 2018: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/30227120/metabolic-profiling-associated-with-autophagy-of-human-placenta-derived-mesenchymal-stem-cells-by-chemical-isotope-labeling-lc-ms
#9
Yanni Sun, Deying Chen, Jingqi Liu, Yanping Xu, Xiaowei Shi, Xian Luo, Qiaoling Pan, Jiong Yu, Jinfeng Yang, Hongcui Cao, Liang Li, Lanjuan Li
Autophagy has been reported to have a pivotal role in maintaining stemness, regulating immunomodulation and enhancing the survival of mesenchymal stem cells (MSCs). However, the effect of autophagy on MSC metabolism is largely unknown. Here, we report a workflow for examining the impact of autophagy on human placenta-derived MSC (hPMSC) metabolome profiling with chemical isotope labeling (CIL) LC-MS. Rapamycin or 3-methyladenine was successfully used to induce or inhibit autophagy, respectively. Then, 12 C- and 13 C-dansylation labeling LC-MS were used to profile the amine/phenol submetabolome...
November 1, 2018: Experimental Cell Research
https://www.readbyqxmd.com/read/30198338/mri-tracking-of-polyethylene-glycol-coated-superparamagnetic-iron-oxide-labelled-placenta-derived-mesenchymal-stem-cells-toward-glioblastoma-stem-like-cells-in-a-mouse-model
#10
Fei-Ting Hsu, Zung-Hang Wei, Yogi Chang-Yo Hsuan, Willie Lin, Yu-Chin Su, Chia-Hui Liao, Chia-Ling Hsieh
Mesenchymal stem cells (MSCs) that display homing and infiltration properties towards tumor cells are a promising cellular targeting vector for brain tumor therapy but are limited to local-regional delivery in current preclinical models. Here, we investigated whether placenta-derived MSCs (P-MSCs) are a superior cellular vector for systemic targeting of glioblastoma stem-like cells (GSCs), with an imaging modality to real-time monitor the trafficking P-MSCs to glioblastoma sites. Results demonstrated that P-MSCs had greater migratory activity towards GSCs and across blood-brain barrier compared with bone marrow-derived MSCs, and this activity was enhanced by hypoxia precondition...
September 8, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/30189195/maternal-malnourishment-induced-upregulation-of-fetuin-b-blunts-nephrogenesis-in-the-low-birth-weight-neonate
#11
May M Rabadi, Wasan Abdulmahdi, Lauren Nesi, Edson Jules, Yara Marghani, Eden Sheinin, Jessica Tilzer, Sanjeev Gupta, Stephen Chen, Nicholas D Cassimatis, Mark Lipphardt, Piotr B Kozlowski, Brian B Ratliff
Maternal undernutrition during pregnancy (MUN) often leads to low birth weight (LBW) neonates that have a reduced total nephron endowment, leaving these neonates susceptible to kidney disease throughout their lives. For reasons unknown, these LBW neonates have impaired kidney development due to a severe reduction in renal SIX2+ stem cells during nephrogenesis. Using a mouse model of MUN, we investigated SIX2+ stem cell reduction in the LBW neonate. Significant upregulation of the protein fetuin-B (measured by PCR and immunoblotting) in the MUN mother's placenta, organs and circulation yielded a 3-fold increase of this protein in the embryonic kidney...
November 1, 2018: Developmental Biology
https://www.readbyqxmd.com/read/30136736/establishment-of-an-in-ovo-chick-embryo-yolk-sac-membrane-ysm-assay-for-pilot-screening-of-potential-angiogenic-and-anti-angiogenic-agents
#12
Muhammad Nihad As, Rucha Deshpande, Vaijayanti P Kale, Ramesh R Bhonde, Savita P Datar
Angiogenesis, the process of new blood vessel formation from pre-existing vessels, is essential for growth and development. Development of drugs that can accelerate or decelerate angiogenesis in the context of various diseases requires appropriate preclinical screening. As angiogenesis involves complex cellular and molecular processes, in vivo studies are superior to in vitro investigations. Conventional in vitro, in vivo, and ex ovo models of angiogenesis are time consuming and tedious, and require sophisticated infrastructure for embryo culture...
August 23, 2018: Cell Biology International
https://www.readbyqxmd.com/read/30118197/enhanced-therapeutic-effects-of-mesenchymal-stem-cell-derived-exosomes-with-an-injectable-hydrogel-for-hindlimb-ischemia-treatment
#13
Kaiyue Zhang, Xiangnan Zhao, Xiaoniao Chen, Yongzhen Wei, Wei Du, Yuebing Wang, Linan Liu, Weian Zhao, Zhibo Han, Deling Kong, Qiang Zhao, Zhikun Guo, Zhongchao Han, Na Liu, Fengxia Ma, Zongjin Li
Mesenchymal stem cell (MSC)-derived exosomes have been recognized as new candidates for cell-free treatment of various diseases. However, maintaining the retention and stability of exosomes over time in vivo after transplantation is a major challenge in the clinical application of MSC-derived exosomes. Here, we investigated if human placenta-derived MSC-derived exosomes incorporated with chitosan hydrogel could boost the retention and stability of exosomes and further enhance their therapeutic effects. Our results demonstrated that chitosan hydrogel notably increased the stability of proteins and microRNAs in exosomes, as well as augmented the retention of exosomes in vivo as confirmed by Gaussia luciferase imaging...
September 12, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/30106038/amnion-epithelial-cells-a-novel-therapy-for-ischemic-stroke
#14
Megan A Evans, Brad R S Broughton, Grant R Drummond, Henry Ma, Thanh G Phan, Euan M Wallace, Rebecca Lim, Christopher G Sobey
Stroke is a leading cause of death and disability and new therapies are desperately needed. Given the complex nature of ischemic brain injury, it has been postulated that cell-based therapies may be useful. However, cell resources, invasive extraction procedures, immunological rejection, tumorigenesis and ethical challenges make it unlikely that many stem cell types could serve as a practical source for therapy. By contrast, these issues do not pertain to human amnion epithelial cells (hAECs), which are placenta-derived stem cells...
August 2018: Neural Regeneration Research
https://www.readbyqxmd.com/read/30096149/transcription-factor-ascl2-is-required-for-development-of-the-glycogen-trophoblast-cell-lineage
#15
Aaron B Bogutz, Rosemary Oh-McGinnis, Karen J Jacob, Rita Ho-Lau, Ting Gu, Marina Gertsenstein, Andras Nagy, Louis Lefebvre
The basic helix-loop-helix (bHLH) transcription factor ASCL2 plays essential roles in diploid multipotent trophoblast progenitors, intestinal stem cells, follicular T-helper cells, as well as during epidermal development and myogenesis. During early development, Ascl2 expression is regulated by genomic imprinting and only the maternally inherited allele is transcriptionally active in trophoblast. The paternal allele-specific silencing of Ascl2 requires expression of the long non-coding RNA Kcnq1ot1 in cis and the deposition of repressive histone marks...
August 2018: PLoS Genetics
https://www.readbyqxmd.com/read/30086473/nostrin-a-novel-modulator-of-trophoblast-giant-cell-differentiation
#16
Shreeta Chakraborty, Rupasri Ain
Differentiation-dependent expression of NOSTRIN in murine trophoblast cells prompted investigation on NOSTRIN's function in trophoblast differentiation. We show here that NOSTRIN levels increased in both mouse and rat placenta during gestation. NOSTRIN expression was not co-related to expression of eNOS precluding its eNOS mediated function. NOSTRIN transcripts were identified in trophoblast cells of the placenta, predominantly in trophoblast giant cells (TGC). Precocious over-expression of NOSTRIN during differentiation of trophoblast stem cells led to up-regulation of genetic markers associated with invasion (Prl4a1, Prl2a1) and TGC formation (Prl2c2, Prl3d1, Prl3b1)...
August 2018: Stem Cell Research
https://www.readbyqxmd.com/read/30079806/aberrant-imprinting-in-mouse-trophoblast-stem-cells-established-from-somatic-cell-nuclear-transfer-derived-embryos
#17
Michiko Hirose, Masashi Hada, Satoshi Kamimura, Shogo Matoba, Arata Honda, Kaori Motomura, Narumi Ogonuki, Hossam H Shawki, Kimiko Inoue, Satoru Takahashi, Atsuo Ogura
Although phenotypic abnormalities frequently appear in the placenta following somatic cell nuclear transfer (SCNT), mouse trophoblast stem cells (TSCs) established from SCNT embryos reportedly show no distinct abnormalities compared with those derived from normal fertilization. In this study, we reexamined SCNT-TSCs to identify their imprinting statuses. Placenta-specific maternally imprinted genes (Gab1, Slc38a4, and Sfmbt2) consistently showed biallelic expression in SCNT-TSCs, suggesting their loss of imprinting (LOI)...
August 23, 2018: Epigenetics: Official Journal of the DNA Methylation Society
https://www.readbyqxmd.com/read/30074161/conditioned-medium-from-human-umbilical-vein-endothelial-cells-promotes-proliferation-migration-invasion-and-angiogenesis-of-adipose-derived-stem-cells
#18
Ming-Lian Luo, Xiao-Ping Liu, Fang Wang, Xiao-Xia Liu, Wei-Fang Liu, Di Wu, Hui Tao, Rong-Li Wang, Yin Zhao, Jian-Wen Zhu, Li Zou
Preeclampsia (PE) is a pregnancy-specific hypertensive complication, closely related to endothelial dysfunction. Adipose derived stem cells (ADSCs) have the capacity to differentiate into endothelial cells for vascular repair. Therefore, we hypothesized that induced endothelial differentiation of ADSCs might hold great potential for the treatment of PE. In this study, the primary ADSCs and human umbilical vein endothelial cells (HUVECs) were isolated by the collagenase digestion method. The supernatant of HUVECs was collected from the first generation of cells...
February 2018: Current medical science
https://www.readbyqxmd.com/read/30067525/importance-of-stem-cell-transplantation-in-cleft-lip-and-palate-surgical-treatment-protocol
#19
Marcelo Paulo Vaccari Mazzetti, Nivaldo Alonso, Ryane Schmidt Brock, Alexandre Ayoub, Sally Mizukami Massumoto, Lilian Piñero Eça
Cleft lip and palate is a congenital malformation that requires a multidisciplinary treatment that evolves pediatrician, obstetrics, fetal medicine, genetics, plastic surgery, orthodontics, speech therapist, nursery, and psychology. Actually, the authors believe that it could be possible to ad protocols to use stem cells.The intrauterine diagnosis leads to preborn parental orientation and better parental collaboration to accept a precocious multidisciplinary treatment. After birth the authors' protocol is: orthodontic devices, phonoaudiology, and surgical procedures...
July 31, 2018: Journal of Craniofacial Surgery
https://www.readbyqxmd.com/read/30021360/protective-effect-of-human-umbilical-cord-mesenchymal-stem-cell-exosomes-on-preserving-the-morphology-and-angiogenesis-of-placenta-in-rats-with-preeclampsia
#20
Zhi-Hui Xiong, Jing Wei, Meng-Qi Lu, Mei-Yuan Jin, Hong-Ling Geng
AIM: This study aims to investigate the effect of human umbilical cord mesenchymal stem cell exosomes (hucMSC-Ex) on placental tissue and angiogenesis in rats with preeclampsia (PE). METHOD: The expression of MSC surface markers were identified by flow cytometry. Alizarin red staining and oil red O staining were used to examine osteogenic and adipogenic differentiation of hucMSCs. Western blotting was used to determine expressions of CD63 and CD81 in hucMSC-Ex. PE rat models were established using endothelial nitric oxide synthase, eNOS)NG -Nitro-l-arginine Methyl Ester, which were then treated with exosome (Exo) of low dosage (L-Exo), Exo of medium dosage (M-Exo) and Exo of high dosage (H-Exo)...
September 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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