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Farideh Tabatabaei Yazdi, Abbas Tanhaeian, Marjan Azghandi, Alireza Vasiee, Behrooz Alizadeh Behbahani, Seyed Ali Mortazavi, Sahar Roshanak
The antimicrobial peptide Thrombocidin-1 (TC-1) isolated from human blood that derived from NAP-2 by deleting of two amino acids from C-terminal region. In this study, a C-terminal 6 _ His tagged recombinant TC-1 was expressed as a secreted peptide in Pichia pastoris, for the first time. The recombinant P. pastoris was inoculated in to BMMY culture medium, incubation with 5 μl/ml absolute methanol for 72 h at 30 °C. The TC-1 peptide was concentrated with nickel affinity chromatography and electrophoresis on 16% acrylamide gels...
December 1, 2018: Microbial Pathogenesis
Adrianna Grzelak, Ingrid Polakova, Jana Smahelova, Julie Vackova, Lucie Pekarcikova, Ruth Tachezy, Michal Smahel
Combined immunotherapy constitutes a novel, advanced strategy in cancer treatment. In this study, we investigated immunotherapy in the mouse TC-1/A9 model of human papillomavirus type 16 (HPV16)-associated tumors characterized by major histocompatibility complex class I (MHC-I) downregulation. We found that the induction of a significant anti-tumor response required a combination of DNA vaccination with the administration of an adjuvant, either the synthetic oligodeoxynucleotide ODN1826, carrying immunostimulatory CpG motifs, or α-galactosylceramide (α-GalCer)...
November 21, 2018: International Journal of Molecular Sciences
Jinyu Li, Adila Aipire, Huixin Zhao, Pengfei Yuan, Jinyao Li
We previously found that Pleurotus ferulae polysaccharides (PFPS) improved the maturation and function of dendritic cells (DCs). In this study, we investigated the effects of PFPS on the antitumor efficacy of therapeutic human papillomavirus (HPV) DC-based vaccine. PFPS stimulated DCs pulsed with HPV E6/E7 peptides were used to treat tumor mice on day 5 & 12 (HPV + PFPS-DCs early) and day 12 & 19 (HPV + PFPS-DCs late) after TC-1 cell injection. Compared to control group, both HPV + PFPS-DCs early and HPV + PFPS-DCs late strategies significantly inhibited tumor growth, which was significantly correlated with the increased activation status of both CD4+ and CD8+ T cells, the decreased frequencies of myeloid-derived suppressor cells, and the induction of HPV-specific CD8+ T cell responses...
November 14, 2018: Human Vaccines & Immunotherapeutics
A R Khosravi, S Alheidary, D Nikaein, N Asghari
Aspergillus fumigatus conidia are the most prevalent indoors fungal allergens. The interaction between Aspergillus antigens and lung epithelial cells (LECs) result in innate immune functions. The association between Aspergillus conidia and allergic reactions, like allergic bronchopulmonary aspergillosis (ABPA) and asthma have been repeatedly reported. Since conventional therapies for allergy and asthma are limited, finding new promising treatments are inevitable. This study was designed to evaluate the effect of A...
October 22, 2018: Journal de Mycologie Médicale
Miho Itoi, Atsushi Okazawa, Jun-Ichi Yamaura, Sachiko Maki, Tokutaro Komatsu, Isabelle Maurin, Epiphane Codjovi, Kamel Boukheddaden, Norimichi Kojima
The structural properties of the iron mixed-valence complex ( n-C3 H7 )4 N[FeII FeIII (dto)3 ] (dto = dithiooxalato, C2 O2 S2 ) have been investigated by single-crystal X-ray diffraction (SCXRD) at low temperatures. ( n-C3 H7 )4 N[FeII FeIII (dto)3 ] has two-dimensional (2D) honeycomb layers consisting of alternating FeII and FeIII arrays bonded by bis-bidentate dithiooxalato ligands. Upon cooling, a superlattice structure with q = (1/3, 1/3, 0) was observed below 260 K, which corresponds to an order-disorder transition of the ( n-C3 H7 )4 N+ ions between the honeycomb layers...
November 5, 2018: Inorganic Chemistry
Bruce C Koppen, Patrick P G Mulder, Leonie de Boer, Martijn Riool, Jan W Drijfhout, Sebastian A J Zaat
Antibiotic resistance and biofilm formation are the major reasons for failure in treatment of bacterial infections. We therefore aimed to identify synergistic combinations of conventional antibiotics and novel Synthetic Antimicrobial and Antibiofilm Peptides (SAAPs) inspired by the structures of the natural human cationic peptides LL-37 and thrombocidin-1 (TC-1). The LL-37-inspired lead peptide SAAP-148 was combined with antibiotics of different classes against Staphylococcus aureus and showed synergy with teicoplanin...
October 10, 2018: International Journal of Antimicrobial Agents
Zhengxin Xie, Jun Tang, Xiangwei Wu, Xuede Li, Rimao Hua
The antibiotic tetracycline (TC) is widespread in surface waters, but few data are available regarding its adverse effects on aquatic insects. In this study, we investigated the bioconcentration, metabolism, and effects of TC on Chironomus riparius larvae exposed to different concentrations of TC (1.83, 18.5 and 174 μg L-1 ) for 48 h. The bioconcentration factors were 3.65, 0.74 and 0.23 in larvae with exposure to 1.83, 18.5 and 174 μg L-1 TC, respectively. High concentration ratios of the metabolites anhydrotetracycline (0...
February 1, 2019: Science of the Total Environment
Young Seob Kim, Hyun Jin Park, Jung Hwa Park, Eun Ji Hong, Gun-Young Jang, In Duk Jung, Hee Dong Han, Seung-Hyun Lee, Manh-Cuong Vo, Je-Jung Lee, Andrew Yang, Emily Farmer, T-C Wu, Tae Heung Kang, Yeong-Min Park
Dendritic cell (DC)-based vaccines are recognized as a promising immunotherapeutic strategy against cancer. Various adjuvants are often incorporated to enhance the modest immunogenicity of DC vaccines. More specifically, many of the commonly used adjuvants are derived from bacteria. In the current study, we evaluate the use of apoptosis inhibitor 5 (API5), a damage-associated molecular pattern expressed by many human cancer cells, as a novel DC vaccine adjuvant. We showed that API5 can prompt activation and maturation of DCs and activate NFkB by stimulating the Toll-like receptor signaling pathway...
2018: Oncoimmunology
Yuki Fukushima, Masanori Someya, Kensei Nakata, Masakazu Hori, Mio Kitagawa, Tomokazu Hasegawa, Takaaki Tsuchiya, Toshio Gocho, Hikaru Ikeda, Yoshihiko Hirohashi, Toshihiko Torigoe, Shintaro Sugita, Tadashi Hasegawa, Tetsuo Himi, Koh-Ichi Sakata
BACKGROUND AND PURPOSE: To investigate influences of proteins involved with tumor immunity on outcomes of radiotherapy for oropharyngeal squamous cell carcinoma (OPSCC). MATERIAL AND METHODS: We performed immunohistochemical staining to examine expressions of p16 and proteins involved with tumor immunity in 92 OPSCC patients treated with radiotherapy. RESULTS: Patients with abundant infiltrating CD8-positive cells had the significantly better overall survival (OS) rate than patients with fewer CD8-positive cells (p = 0...
November 2018: Radiotherapy and Oncology: Journal of the European Society for Therapeutic Radiology and Oncology
Ho-Young Jang, Baek-Sang Han, Byungsuk Kwon, Jeong-Im Sin
The role of chemotherapeutic agents in tumor immunotherapy is still controversial. In this study, we test using a TC-1 tumor model whether gemcitabine plus E7 peptide vaccine regimens (E7 peptides+CpG-ODN+anti-4-1BB Abs) may result in tumor cure in mice with large established tumors, with a focus on their effects on Ag-specific cytotoxic T lymphocyte (CTL) and myeloid-derived suppressor cell levels. Gemcitabine inhibited tumor growth by its direct cytotoxicity to tumor cells in vivo. E7 peptide vaccine regimens enhanced Ag-specific CTL lytic and antitumor therapeutic activity...
October 2018: DNA and Cell Biology
Olena Sapega, Romana Mikyšková, Jana Bieblová, Blanka Mrázková, Zdeněk Hodný, Milan Reiniš
Cellular senescence is the process of the permanent proliferative arrest of cells in response to various inducers. It is accompanied by typical morphological changes, in addition to the secretion of bioactive molecules, including proinflammatory cytokines and chemokines [known as the senescence-associated secretory phenotype (SASP)]. Thus, senescent cells may affect their local environment and induce a so-called 'bystander' senescence through the state of SASP. The phenotypes of senescent cells are determined by the type of agent inducing cellular stress and the cell lineages...
November 2018: International Journal of Oncology
Jin Qiu, Shiwen Peng, Andrew Yang, Ying Ma, Liping Han, Max A Cheng, Emily Farmer, Chien-Fu Hung, T-C Wu
Purpose : Mucosal immunization is suggested to be crucial for controlling tumors in the mucosal region; however, therapeutic DNA vaccination with electroporation in various mucosal sites has yet to become clinically adaptable. Since tumor-draining lymph nodes (tdLNs) have been suggested as immune-educated sites that can be utilized to mount a potent antitumor immune response, we examined whether intramuscular DNA vaccination with electroporation at sites that target the mucosal tdLNs could elicit mucosal immune response to restrict tumor growth...
2018: Oncoimmunology
Xin Lu, Beibei Tang, Qi Zhang, Lizhu Liu, Ruqin Fan, Zhenhua Zhang
Batch experiments were conducted to investigate the adsorption characteristics of tetracycline (TC), and the interactive effects of copper (Cu) on the adsorption of TC onto water hyacinth roots. TC removal efficiency by water hyacinth roots was ranging from 58.9% to 84.6%, for virgin TC, 1:1 TC-Cu and 1:2 TC-Cu. The Freundlich isotherm model and the pseudo-second-order kinetic model fitted the adsorption data well. Thermodynamics parameters ΔG ⁰ for TC were more negative in the TC plus Cu than the TC-only treatments, indicating the spontaneity of TC adsorption increased with increasing of Cu concentrations...
September 11, 2018: International Journal of Environmental Research and Public Health
Amir Hossein Mohseni, Vadood Razavilar, Hossein Keyvani, Mohammad Reza Razavi, Ramazan Ali Khavari-Nejad
The ORFs of both native and codon-optimized E7 genes were successfully fused to SPusp45 signal peptide and expressed by a nisin-controlled gene expression system (NICE) in NZ9000 strains of Lactococcus lactis. Recombinant strains were confirmed by Western blot analysis. To measure immune responses against the E7 antigen, specific-pathogen-free C57BL/6 mice were inoculated with L. lactis harboring pNZ8123-rE7 by oral gavage. Then, specific antibodies and cytokines were measured by Enzyme-linked immunosorbent assay (ELISA) and enzyme-linked immunospot (ELISPOT) assay, respectively...
September 7, 2018: Journal of Medical Virology
Ana C R Moreno, Bruna F M M Porchia, Roberta L Pagni, Patrícia da Cruz Souza, Rafael Pegoraro, Karine B Rodrigues, Tácita B Barros, Luana R de Melo Moraes Aps, Eliseu F de Araújo, Vera L G Calich, Luís C de Souza Ferreira
Immunotherapy has become an important ally in the fight against distinct types of cancer. However, the metabolic plasticity of the tumor environment frequently influences the efficacy of therapeutic procedures, including those based on immunological tools. In this scenario, immunometabolic adjuvants arise as an alternative toward the development of more efficient cancer therapies. Here we demonstrated that the combination of melatonin, a neuroimmunomodulator molecule, and an indoleamine 2,3-dioxygenase (IDO) inhibitor (1-methyl-DL-tryptophan, DL-1MT) improves the efficacy of an immunotherapy (gDE7) targeting human papillomavirus (HPV)-associated tumors...
2018: Frontiers in Immunology
Shunsuke Tomomori, Yukiko Nakano, Hidenori Ochi, Yuko Onohara, Akinori Sairaku, Takehito Tokuyama, Chikaaki Motoda, Hiroya Matsumura, Michitaka Amioka, Naoya Hironobe, Yousaku Ookubo, Shou Okamura, Hiroshi Kawazoe, Kazuaki Chayama, Yasuki Kihara
INTRODUCTION: The single nucleotide polymorphism (SNP) rs2106261 in the transcription factor gene ZFHX3 (16q22), a major regulator of inflammation, has been reported linking to atrial fibrillation (AF) by genome-wide association studies. Inflammation is known to be a strong predictor of atrial fibrillation recurrence after ablation, so we examined the association of the ZFHX3 SNP rs2106261 to inflammation marker expression and recurrence after AF ablation. METHODS: We genotyped ZFHX3 SNP rs2106261 and compared the minor (T) allele frequency between 362 paroxysmal AF (PAF) patients underwent pulmonary vein isolation (PVI) and 627 non-AF controls...
2018: PloS One
Gabriele Galliverti, Mélanie Tichet, Sonia Domingos-Pereira, Sylvie Hauert, Denise Nardelli-Haefliger, Melody A Swartz, Douglas Hanahan, Stephan Wullschleger
Treatment of patients bearing human papillomavirus (HPV)-related cancers with synthetic long-peptide (SLP) therapeutic vaccines has shown promising results in clinical trials against premalignant lesions, whereas responses against later stage carcinomas have remained elusive. We show that conjugation of a well-documented HPV-E7 SLP to ultra-small polymeric nanoparticles (NP) enhances the antitumor efficacy of therapeutic vaccination in different mouse models of HPV+ cancers. Immunization of TC-1 tumor-bearing mice with a single dose of NP-conjugated E7LP (NP-E7LP) generated a larger pool of E7-specific CD8+ T cells with increased effector functions than unconjugated free E7LP...
November 2018: Cancer Immunology Research
Jung Min Shin, Seok Ho Song, N Vijayakameswara Rao, Eun Sook Lee, Hyewon Ko, Jae Hyung Park
Background: Antigen-specific cytotoxic T lymphocytes (CTLs), which eliminate target cells bearing antigenic peptides presented by surface major histocompatibility complex (MHC) class I molecules, play a key role in cancer immunotherapy. However, the majority of tumors are not immunologically rejected since they express self-antigens which are not recognized by CTLs as foreign. To foreignize these tumors for CTL-mediated immunological rejection, it is essential to develop carriers that can effectively deliver foreign antigens to cancer cells...
2018: Biomaterials Research
Samaneh Kameli, Ali Mehrizad
Recent improvements based on heterojunction nanocomposites have opened new possibilities in photocatalysis. In this research, an ultrasound assisted co-precipitation method was used to fabricate silver, zinc sulfide, and reduced graphene oxide (Ag-ZnS/rGO) nanocomposite, and characterization results indicated that 3% Ag-ZnS spherical nanoparticles are successfully embedded in rGO matrix. The potential of the Ag-ZnS/rGO, as a visible light active photocatalyst, was assessed through optimizing degradation of Tetracycline (TC) by response surface methodology...
August 14, 2018: Photochemistry and Photobiology
Xi Yang, Xianzhong Yu, Yanzhang Wei
Many of the cytokine-based cancer immunotherapies are hindered by the devastating side effects of systemic delivery of the cytokines. To address this problem, we previously described a novel approach to locally achieve high doses of interleukin-12 (IL-12) in tumors and demonstrated that bi-functional fusion protein mIL-12/FasTI expressed by stable clones of TC-1 cells efficiently suppressed tumor proliferation by activating natural killer (NK) cells and other cytolytic killer cells and sending apoptotic signals into tumor cells...
2018: PloS One
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