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Teuta Pilizota, Ya-Tang Yang
With the emergence of inexpensive 3D printing technology, open-source platforms for electronic prototyping and single-board computers, "Do it Yourself" (DIY) approaches to the cultivation of microbial cultures are becoming more feasible, user-friendly, and thus wider spread. In this perspective, we survey some of these approaches, as well as add-on solutions to commercial instruments for synthetic and system biology applications. We discuss different cultivation designs, including capabilities and limitations...
2018: Frontiers in Microbiology
Sisi Li, Diying Shen, Xiaoping Guo, Chan Liao, Yongmin Tang
Antibody-targeting therapy has drawn great interests to the hematologists and oncologists. 3A4, a novel antibody recognizing human CD45RA antigen, is a new target molecule for leukemias and holds a therapeutic potential for myeloid lineage leukemias. However, murine antibodies cannot be safely used in patients because of their strong immune reaction, humanization of the antibodies interested will be an important development step for therapeutic purpose. The aim of this study was to engineer the mouse 3A4 and to investigate the biological activity of its chimeric form...
August 10, 2018: DNA and Cell Biology
Katharine D Barnard, Ralph Ziegler, David C Klonoff, Katarina Braune, Bettina Petersen, Til Rendschmidt, Daniel Finan, Aaron Kowalski, Lutz Heinemann
Biomedical outcomes for people with diabetes remain suboptimal for many. Psychosocial care in diabetes does not fare any better. "Artificial pancreas" (also known as "closed-loop" and "automated insulin delivery") systems present a promising therapeutic option for people with diabetes (PWD)-simultaneously improving glycemic outcomes, reducing the burden of self-management, and improving health-related quality of life. In recent years there has emerged a growing movement of PWD innovators rallying behind the mantra #WeAreNotWaiting, developing "do-it-yourself artificial pancreas systems (DIY APS)...
August 6, 2018: Journal of Diabetes Science and Technology
Laurent M Arsac, Véronique Deschodt-Arsac
Fractal physiology demonstrated growing interest over the last decades among physiologists, neuroscientists, and clinicians. Many physiological systems coordinate themselves for reducing variability and maintain a steady state. When recorded over time, the output signal exhibits small fluctuations around a stable value. It is becoming increasingly clear that these fluctuations, in most free-running healthy systems, are not simply due to uncorrelated random errors and possess interesting properties, one of which is the property of fractal dynamics...
September 1, 2018: Advances in Physiology Education
Kevin S Paulsen, Yanxiang Deng, Aram J Chung
Complex-shaped microparticles can enhance applications in drug delivery, tissue engineering, and structural materials, although techniques to fabricate these particles remain limited. A microfluidics-based process called optofluidic fabrication that utilizes inertial flows and ultraviolet polymerization has shown great potential for creating highly 3D-shaped particles in a high-throughput manner, but the particle dimensions are mainly at the millimeter scale. Here, a next generation optofluidic fabrication process is presented that utilizes on-the-fly fabricated multiscale fluidic channels producing customized sub-100 µm 3D-shaped microparticles...
July 2018: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
Jim Kling
In the version of this Technology Feature originally published, André Maia Chagas' lab affiliation was omitted and the name of his institution, The Centre for Integrative Neuroscience, was incorrectly written. This has now been corrected.
August 2018: Lab Animal
Ruizan Shi, Diying Zhu, Zehui Wei, Naijie Fu, Chang Wang, Linhong Liu, Huifeng Zhang, Yueqin Liang, Jianfeng Xing, Xuening Wang, Yan Wang
AIMS: Endothelial-to-mesenchymal transition (EndoMT) was shown to lead to endothelial cell (EC) dysfunction in pulmonary arterial hypertension (PAH). Baicalein was reported to inhibit epithelial-to-mesenchymal transition (EMT), a biological process that has many regulatory pathways in common with EndoMT. Whether it can attenuate PAH by inhibiting EndoMT remains obscure. MAIN METHODS: PAH was induced by a single subcutaneous injection of MCT (60 mg/kg) in male Sprague Dawley rats...
August 15, 2018: Life Sciences
Morgan Meyer
Do-it-yourself (DIY) biology and medicine are based on various practices and logics: amateur and DIY practices, the ethics of hacking and open source, the drive to domesticate molecular biology and genetics, the ideal of participation and citizen science. The article shows that this democratization is a process that is at once spatial (construction of new spaces), technical (creative workarounds equipment), social (establishment of accessible networks/laboratories) and political. It is therefore through their practices, gestures and questions - tinkering, experimenting, working around, amaterializing, ethicizing, comparing, valuating, etc...
May 2018: Médecine Sciences: M/S
Mitchell J Geer, Johanna P van Geffen, Piraveen Gopalasingam, Timo Vögtle, Christopher W Smith, Silke Heising, Marijke J E Kuijpers, Bibian M E Tullemans, Gavin E Jarvis, Johannes A Eble, Mark Jeeves, Michael Overduin, Johan W M Heemskerk, Alexandra Mazharian, Yotis A Senis
The immunoreceptor tyrosine-based inhibitory motif (ITIM)-containing receptor G6b-B has emerged as a key regulator of platelet homeostasis. However, it remains unclear how it mediates its effects. Tyrosine phosphorylation of the ITIM and immunoreceptor tyrosine-based switch motif (ITSM) within the cytoplasmic tail of G6b-B provides a docking site for SH2 domain-containing protein-tyrosine phosphatases Shp1 and Shp2, which are also critical regulators of platelet production and function. In this study, we investigate the physiological consequences of uncoupling G6b-B from Shp1 and Shp2...
June 11, 2018: Blood
Brandon G Wong, Christopher P Mancuso, Szilvia Kiriakov, Caleb J Bashor, Ahmad S Khalil
Precise control over microbial cell growth conditions could enable detection of minute phenotypic changes, which would improve our understanding of how genotypes are shaped by adaptive selection. Although automated cell-culture systems such as bioreactors offer strict control over liquid culture conditions, they often do not scale to high-throughput or require cumbersome redesign to alter growth conditions. We report the design and validation of eVOLVER, a scalable do-it-yourself (DIY) framework, which can be configured to carry out high-throughput growth experiments in molecular evolution, systems biology, and microbiology...
August 2018: Nature Biotechnology
Jim Kling
No abstract text is available yet for this article.
June 2018: Lab Animal
(no author information available yet)
No abstract text is available yet for this article.
May 11, 2018: British Dental Journal
Maria P Martinez, Kurunthachalam Kannan
The determination of oxidative stress biomarkers (OSBs) is useful for the assessment of health status and progress of diseases in humans. Whereas previous methods for the determination of OSBs in urine were focused on a single marker, in this study, we present a method for simultaneous determination of biomarkers of oxidative damage to lipids, proteins, and DNA. 2,4-Dinitrophenylhydrazine (DNPH) derivatization followed by solid phase extraction (SPE) and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) allowed the determination of 8-hydroxy-2'-deoxyguanosine (8-OHdG), o- o'-dityrosine (diY), malondialdehyde (MDA), and four F2 -isoprostane isomers: 8-iso-prostaglandinF2α (8-PGF2α ), 11β-prostaglandinF2α (11-PGF2α ), 15( R)-prostaglandinF2α (15-PGF2α ), and 8-iso,15( R)-prostaglandinF2α (8,15-PGF2α ) in urine...
June 5, 2018: Environmental Science & Technology
S Jadun, L Monaghan, J Darcey
No abstract text is available yet for this article.
April 27, 2018: British Dental Journal
Yana Fedyanova
No abstract text is available yet for this article.
March 12, 2018: CMAJ: Canadian Medical Association Journal, Journal de L'Association Medicale Canadienne
Wei-Kai Wu, Chieh-Chang Chen, Suraphan Panyod, Rou-An Chen, Ming-Shiang Wu, Lee-Yan Sheen, Shan-Chwen Chang
Although great interest has been displayed by researchers in the contribution of gut microbiota to human health, there is still no standard protocol with consensus to guarantee the sample quality of metagenomic analysis. Here we reviewed existing methodology studies and present suggestions for optimizing research pipeline from fecal sample collection to DNA extraction. First, we discuss strategies of clinical metadata collection as common confounders for microbiome research. Second, we propose general principles for freshly collected fecal sample and its storage and share a DIY stool collection kit protocol based on the manual procedure of Human Microbiome Project (HMP)...
February 25, 2018: Journal of the Formosan Medical Association, Taiwan Yi Zhi
Patricia A Beddows, Edward K Mallon
A low-cost data logging platform is presented that provides long-term operation in remote or submerged environments. Three premade "breakout boards" from the open-source Arduino ecosystem are assembled into the core of the data logger. Power optimization techniques are presented which extend the operational life of this module-based design to >1 year on three alkaline AA batteries. Robust underwater housings are constructed for these loggers using PVC fittings. Both the logging platform and the enclosures, are easy to build and modify without specialized tools or a significant background in electronics...
February 9, 2018: Sensors
Michael S Engel, Diying Huang, Chenyang Cai, Abdulaziz S Alqarni
A new braconid wasp from the Upper Cretaceous (Cenomanian) amber of the Hukawng Valley in Kachin State, Myanmar is described and figured from a unique female. Seneciobracon novalatus Engel & Huang, gen. et sp. n. , is placed in a distinct subfamily, Seneciobraconinae Engel & Huang, subfam. n. , owing to the presence of a unique combination of primitive protorhyssaline-like traits, with an otherwise more derived wing venation. The fossil is discussed in the context of other Cretaceous Braconidae.
2018: ZooKeys
Shabir Ahmad, Lei Hang, Do Hyeun Kim
The Do-It-Yourself (DIY) vision for the design of a smart and customizable IoT application demands the involvement of the general public in its development process. The general public lacks the technical knowledge for programming state-of-the-art prototyping and development kits. The latest IoT kits, for example, Raspberry Pi, are revolutionizing the DIY paradigm for IoT, and more than ever, a DIY intuitive programming interface is required to enable the masses to interact with and customize the behavior of remote IoT devices on the Internet...
February 6, 2018: Sensors
Eric Smalley
No abstract text is available yet for this article.
February 6, 2018: Nature Biotechnology
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