keyword
https://read.qxmd.com/read/38484639/optimization-of-elution-conditions-and-comparison-of-emerging-biocompatible-columns-on-the-resolving-power-and-detection-sensitivity-of-oligonucleotides-by-ion-pairing-reversed-phase-liquid-chromatography-mass-spectrometry
#21
JOURNAL ARTICLE
Quang-Dong Bui, Tiny Deschrijver, Bart Noten, Willy Verluyten, Nico Vervoort, Sebastiaan Eeltink
A generic performance comparison strategy has been developed to evaluate the impact of mobile-phase additives (ion-pairing agent / counter ion systems), distinct stationary phases on resulting resolving power, and MS detectability of oligonucleotides and their critical impurities in gradient IP-RPLC. Stationary-phase considerations included particle type (core-shell vs. fully porous particles), particle diameter, and pore size. Separations were carried out at 60°C to optimize mass transfer (C-term). The incorporation of an active column preheater mitigated thermal mismatches, leading to narrower peaks and overcoming peak splitting...
March 5, 2024: Journal of Chromatography. A
https://read.qxmd.com/read/38484332/electrophoretic-mobility-of-nanoparticles-in-water
#22
JOURNAL ARTICLE
Dmitry V Matyushov
Classical equations for colloidal mobility anticipate linear proportionality between the nanoparticle mobility and zeta potential caused by the combined electrostatics of free charges at the nanoparticle and screening bound charges of the polar solvent. Polarization of the interfacial liquid, either spontaneous due to molecular asymmetry of the solvent (water) or induced by nonelectrostatic (e.g., charge-transfer) interactions, is responsible for a static interface charge adding to the overall electrokinetic charge of the nanoparticle...
March 14, 2024: Journal of Physical Chemistry. B
https://read.qxmd.com/read/38484154/genetically-fused-resilin-like-polypeptide-coiled-coil-bundlemer-conjugates-exhibit-tunable-multistimuli-responsiveness-and-undergo-nanofibrillar-assembly
#23
JOURNAL ARTICLE
Sai S Patkar, Yao Tang, Tianren Zhang, Arriana M Bisram, Jeffery G Saven, Darrin J Pochan, Kristi L Kiick
Peptide-based materials are diverse candidates for self-assembly into modularly designed and stimuli-responsive nanostructures with precisely tunable compositions. Here, we genetically fused computationally designed coiled coil-forming peptides to the N- and C-termini of compositionally distinct multistimuli-responsive resilin-like polypeptides (RLPs) of various lengths. The successful expression of these hybrid polypeptides in bacterial hosts was confirmed through techniques such as gel electrophoresis, mass spectrometry, and amino acid analysis...
March 14, 2024: Biomacromolecules
https://read.qxmd.com/read/38481760/using-capillary-electrophoresis-to-identify-anopheline-species-in-routine-sampling-sites
#24
JOURNAL ARTICLE
Estelle Chabanol, Ottavia Romoli, Stanislas Talaga, Yanouk Epelboin, Katy Heu, Ghislaine Prévot, Mathilde Gendrin
In the Anopheles genus, various mosquito species are able to transmit the Plasmodium parasites responsible for malaria, while others are non-vectors. In an effort to better understand the biology of Anopheles species and to quantify transmission risk in an area, the identification of mosquito species collected in the field is an essential but problematic task. Morphological identification requires expertise and cannot be checked after processing samples in a destructive treatment, while sequencing of numerous samples is costly...
March 2024: Ecology and Evolution
https://read.qxmd.com/read/38481696/enhancing-rna-payload-and-temperature-stability-and-activity-with-cationic-peptide-coated-zinc-oxide-nanoparticles
#25
JOURNAL ARTICLE
Robert K DeLong, Juliet Nava-Chavez, Rakshith Kumar, Elza Neelima Mathew, Waithaka Mwangi, Sunyoung Yoon
The lipid nanoparticle (LNP) mRNA vaccine was first tested through clinic but suffered from relatively low RNA payloads and poor temperature stability. Our lab patented a protamine-coated particle approach for temperature-stabilizing DNA vaccines, translating this successfully to the clinic. In subsequent work, we have characterized RNA interaction and delivery by zinc oxide nanoparticles, filing a patent most recently entitled RNA-stabilizing nanoparticles, similarly utilizing protamine-coated zinc oxide nanoparticles for RNA...
March 8, 2024: ACS Pharmacology & Translational Science
https://read.qxmd.com/read/38479336/insights-into-the-fish-protein-degradation-induced-by-the-fish-borne-spoiler-pseudomonas-psychrophila-and-shewanella-putrefaciens-from-whole-genome-sequencing-to-quality-changes
#26
JOURNAL ARTICLE
Shiliang Jia, Zhifang Jia, Jun An, Yicheng Ding, Jie Chang, Yanbo Wang, Xuxia Zhou
The aim of this study is evaluating the protein degradation capacity of specific spoilage organisms (SSOs) Pseudomonas psychrophila and Shewanella putrefaciens in fish flesh during chilled storage and revealing the underlying genes by whole-genome sequencing (WGS). Biochemical and physical tests were performed on fish flesh inoculated with P. psychrophila and S. putrefaciens individually, including textural properties, myofibrillar fragmentation index, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) profiles, free amino acid composition, total volatile basic nitrogen (TVB-N), trichloroacetic acid (TCA) soluble peptides, and muscle microstructure...
March 9, 2024: International Journal of Food Microbiology
https://read.qxmd.com/read/38479154/a-rapid-one-step-affinity-purification-of-c-phycocyanin-from-spirulina-platensis
#27
JOURNAL ARTICLE
Ke Shi, Wei Wang, Jingjing Sun, Chengcheng Jiang, Jianhua Hao
The high-purity phycocyanin has a high commercial value. Most current purification methods of C-phycocyanin involve multiple steps, which are complicated and time-consuming. To solve the problem, this research was studied, and an efficient affinity chromatography purification for C-phycocyanin from Spirulina platensis was developed. Through molecular docking simulation, virtual screening of ligands was performed, and ursolic acid was identified as the specific affinity ligand, which coupled to Affi-Gel 102 gel via 1-ethyl (3-dimethylaminopropyl)-3-carbodiimide, hydrochloride as coupling agent...
March 6, 2024: Journal of Chromatography. A
https://read.qxmd.com/read/38478280/modular-assembly-of-mitochondrial-%C3%AE-barrel-proteins
#28
JOURNAL ARTICLE
Rituparna Bhowmik, Fabian den Brave, Thomas Becker
Mitochondrial β-barrel proteins fulfill crucial roles in the biogenesis and function of the cell organelle. They mediate the import and membrane insertion of proteins and transport of small metabolites and ions. All β-barrel proteins are made as precursors on cytosolic ribosomes and are imported into mitochondria. The β-barrel proteins fold and assemble with partner proteins in the outer membrane. The in vitro import of radiolabelled proteins into isolated mitochondria is a powerful tool to investigate the import of β-barrel proteins, the folding of the β-barrel proteins, and their assembly into protein complexes...
2024: Methods in Molecular Biology
https://read.qxmd.com/read/38478276/analysis-of-transmembrane-%C3%AE-barrel-proteins-by-native-and-semi-native-polyacrylamide-gel-electrophoresis
#29
JOURNAL ARTICLE
Violette Morales, Luis Orenday-Tapia, Raffaele Ieva
Membrane-embedded β-barrel proteins are important regulators of the outer membrane permeability barrier of Gram-negative bacteria. β-barrels are highly structured domains formed by a series of antiparallel β-strands. Each β-strand is locked in position by hydrogen bonds between its polypeptide backbone and those of the two neighbouring strands in the barrel structure. Some transmembrane β-barrel proteins form larger homo- or hetero-multimeric complexes that accomplish specific functions...
2024: Methods in Molecular Biology
https://read.qxmd.com/read/38477690/development-of-an-easy-to-set-up-multiple-heart-cutting-achiral-chiral-lc-lc-method-for-the-analysis-of-branched-chain-amino-acids-in-commercial-tablets
#30
JOURNAL ARTICLE
Ina Varfaj, Ghaid W A Abualzulof, Simone Moretti, Anna Migni, Irene Uda, Laura Goracci, Federica Ianni, Andrea Carotti, Roccaldo Sardella
In this paper, the development and application of a multiple heart-cutting achiral-chiral LC-LC method (mLC-LC) for the analysis of dansylated (Dns) branched-chain amino acids in commercial tablets are described. In the first dimension, a Waters Xbridge RP C18 achiral column was used under gradient conditions with buffered aqueous solution and acetonitrile. The elution order Dns-valine (Dns-Val) < Dns-isoleucine (Dns-Ile) < Dns-leucine (Dns-Leu) turned out with full resolution between adjacent peaks: 7...
March 13, 2024: Electrophoresis
https://read.qxmd.com/read/38477450/proteomic-analysis-of-host-cell-protein-fouling-during-bioreactor-harvesting
#31
JOURNAL ARTICLE
Da Zhang, S Ranil Wickramasinghe, Andrew L Zydney, John P Smelko, Abdullah Loman, April Wheeler, Xianghong Qian
Chinese hamster ovary (CHO) cells are among the most common cell lines used for therapeutic protein production. Membrane fouling during bioreactor harvesting is a major limitation for the downstream purification of therapeutic proteins. Host cell proteins (HCP) are the most challenging impurities during downstream purification processes. The present work focuses on identification of HCP foulants during CHO bioreactor harvesting using reverse asymmetrical commercial membrane BioOptimal™ MF-SL. In order to investigate foulants and fouling behavior during cell clarification, for the first time a novel backwash process was developed to effectively elute almost all the HCP and DNA from the fouled membrane filter...
March 13, 2024: Biotechnology Progress
https://read.qxmd.com/read/38474110/exploring-the-molecular-tapestry-organ-specific-peptide-and-protein-ultrafiltrates-and-their-role-in-therapeutics
#32
JOURNAL ARTICLE
Jakub Peter Slivka, Chris Bauer, Alexander Younsi, Michelle B F Wong, Mike K S Chan, Thomas Skutella
This study aims to characterize the proteome composition of organ-derived protein extracts from rabbits. Protein isolation was performed using soft homogenization and size exclusion via ultrafiltration. The proteome analysis of the ultrafiltrates was conducted using gel electrophoresis, and the mass spectrometry data were subjected to gene ontology analysis. Proteomic profiling revealed comprehensive protein profiles associated with RNA regulation, fatty acid binding, inflammatory response, oxidative stress, and metabolism...
March 1, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38473700/-solanum-lycopersicum-tomato-derived-nanovesicles-accelerate-wound-healing-by-eliciting-the-migration-of-keratinocytes-and-fibroblasts
#33
JOURNAL ARTICLE
Valeria Daniello, Vincenzo De Leo, Maria Lasalvia, Md Niamat Hossain, Annalucia Carbone, Lucia Catucci, Roberto Zefferino, Chiara Ingrosso, Massimo Conese, Sante Di Gioia
Plant-derived nanovesicles have been considered interesting in medicine for their breakthrough biological effects, including those relevant to wound healing. However, tomato-derived nanovesicles (TDNVs) have not been studied for their effects on wound closure yet. TDNVs were isolated from Solanum lycopersicum (var. Piccadilly) ripe tomatoes by ultracentrifugation. Extract (collected during the isolation procedure) and NVs (pellet) were characterized by transmission electron microscopy and laser Doppler electrophoresis...
February 20, 2024: International Journal of Molecular Sciences
https://read.qxmd.com/read/38472986/analytical-validation-of-loss-of-heterozygosity-and-mutation-detection-in-pancreatic-fine-needle-aspirates-by-capillary-electrophoresis-and-sanger-sequencing
#34
JOURNAL ARTICLE
Venkata Arun Timmaraju, Sydney David Finkelstein, Jonathan Adam Levine
Pancreatic cystic disease, including duct dilation, represents precursor states towards the development of pancreatic cancer, a form of malignancy with relatively low incidence but high mortality. While most of these cysts (>85%) are benign, the remainder can progress over time, leading to malignant transformation, invasion, and metastasis. Cytologic diagnosis is challenging, limited by the paucity or complete absence of cells representative of cystic lesions and fibrosis. Molecular analysis of fluids collected from endoscopic-guided fine-needle aspiration of pancreatic cysts and dilated duct lesions can be used to evaluate the risk of progression to malignancy...
February 28, 2024: Diagnostics
https://read.qxmd.com/read/38472786/influence-of-roasting-temperature-on-the-detectability-of-potentially-allergenic-lupin-by-sds-page-elisas-lc-ms-ms-and-real-time-pcr
#35
JOURNAL ARTICLE
Bruno Beyer, Dominik Obrist, Philipp Czarda, Katharina Pühringer, Filip Vymyslicky, Barbara Siegmund, Stefano D'Amico, Margit Cichna-Markl
Seeds of "sweet lupins" have been playing an increasing role in the food industry. Lupin proteins may be used for producing a variety of foods, including pasta, bread, cookies, dairy products, and coffee substitutes. In a small percentage of the population, lupin consumption may elicit allergic reactions, either due to primary sensitization to lupin or due to cross-allergy with other legumes. Thus, lupin has to be declared on commercial food products according to EU food regulations. In this study, we investigated the influence of roasting seeds of the L...
February 23, 2024: Foods (Basel, Switzerland)
https://read.qxmd.com/read/38471979/high-throughput-extraction-and-automatic-purification-of-alternariol-from-edible-and-medicinal-herbs-based-on-aptamer-functionalized-magnetic-nanoparticles
#36
JOURNAL ARTICLE
Ruiqi Yang, Liping Zhao, Fengchao Wang, Jin Chen, Xiao Ma, Yunxia Luan, Weijun Kong
Mycotoxin contamination is widespread in plants and herbs, posing serious threats to the consumer and human health. Of them, alternariol (AOH) has attracted great attention as an "emerging" mycotoxin in medicinal herbs. However, a specific and high-throughput extraction method for AOH is currently lacking. Thus, developing an efficient pre-treatment technique for AOH detection is extremely vital. Here, a novel automated magnetic solid-phase extraction method was proposed for the highly efficient extraction of AOH...
March 2024: Journal of Separation Science
https://read.qxmd.com/read/38471978/ultra-performance-liquid-chromatography-quadrupole-time-of-flight-mass-combined-with-unifi-to-study-the-mechanism-of-tao-hong-si-wu-decoction-in-the-treatment-of-postpartum-blood-stasis
#37
JOURNAL ARTICLE
Mengdie Zhao, Wenwen Xia, Peiliang Zhang, Qingqing Xie, Wenyu Mu, Linfeng Tang, Zhuqing Liu, Lan Han, Daiyin Peng
Postpartum hemorrhage can lead to a variety of maternal complications. Tao Hong Si Wu Decoction (THSWD) is a traditional Chinese medicine used for treating gynecological diseases. However, the active ingredients of THSWD and its pharmacological mechanism of treatment for postpartum blood stasis still remained unclear. In this study, 201 components were identified in THSWD ethanol extract using ultra-performance liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry, including 59 terpenoids and volatile oil, 61 Phenylpropanoids, 41 flavonoids, 22 alkaloids, and other 18 components...
March 2024: Journal of Separation Science
https://read.qxmd.com/read/38471977/multi-dimensional-technology-recent-advances-and-applications-for-biotherapeutic-characterization
#38
REVIEW
Thomas Bouvarel, Julien Camperi, Davy Guillarme
This review provides an overview of the latest advancements and applications in multi-dimensional liquid chromatography coupled with mass spectrometry (mD-LC-MS), covering aspects such as inter-laboratory studies, digestion strategy, trapping column, and multi-level analysis. The shift from an offline to an online workflow reduces sample processing artifacts, analytical variability, analysis time, and the labor required for data acquisition. Over the past few years, this technique has demonstrated sufficient maturity for application across a diverse range of complex products...
March 2024: Journal of Separation Science
https://read.qxmd.com/read/38471974/liquid-chromatography-mass-spectrometry-based-strategy-for-systematic-profiling-of-chemical-components-and-associated-quantitative-analysis-of-quality-markers-in-qi-wei-tong-bi-oral-liquid
#39
JOURNAL ARTICLE
Xiaojun Huang, Qingqing Fei, Sheng Yu, Rongli Qiu, Ting Geng, Xialin Chen, Liang Cao, Zhenzhong Wang, Mingqiu Shan
Qi-Wei-Tong-Bi oral liquid (QWTB), a famous Chinese medicine preparation composed of seven crude drugs has a good therapeutic effect on rheumatoid arthritis and is widely used in China. However, its chemical composition and quality control have not been comprehensively and systematically investigated. In this study, high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry was employed for its chemical profiling. As a result, 100 components were chemically characterized...
March 2024: Journal of Separation Science
https://read.qxmd.com/read/38471971/covalent-organic-framework-layered-double-hydroxide-composite-coated-poly-ether-ether-ketone-jacket-for-stir-bar-sorptive-extraction-of-sudan-dyes
#40
JOURNAL ARTICLE
Zhenfu He, Shumin Ma, Peiqi Huang, Qionghuan Liang, Rong Wang
A novel coating for stir bar sorptive extraction was developed by growing a covalent organic framework, TpPa-1 (derived from phenylenediamine and 1,3,5-trimethylphloroglucinol), onto the surface of Ni-Al layered double hydroxide. Using a poly(ether ether ketone) tube as the supporting substrate, a TpPa-1/layered double hydroxide-coated stir bar was fabricated and demonstrated excellent extraction performance for Sudan dyes. Notably, its extraction efficiency significantly exceeded that of stir bars modified with only TpPa-1 or Ni-Al layered double hydroxide...
March 2024: Journal of Separation Science
keyword
keyword
167776
2
3
Fetch more papers »
Fetching more papers... Fetching...
Remove bar
Read by QxMD icon Read
×

Save your favorite articles in one place with a free QxMD account.

×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"

We want to hear from doctors like you!

Take a second to answer a survey question.