keyword
https://read.qxmd.com/read/38492290/novel-approach-over-template-molecule-elimination-in-molecularly-imprinted-polymers-by-using-heat-activated-persulfate
#1
JOURNAL ARTICLE
Juan I Calbanese, Mario D Contin, Pablo R Bonelli, Ana L Cukierman, Valeria P Tripodi
This study proposes a new alternative for template removal from molecularly imprinted polymers by heat activated persulfate. It is known that trace amounts of template molecule remains in the polymer network after extraction by current methodologies leading to bleeding and incomplete removal of template which could compromise final determination of target analytes especially in trace analysis. A previously developed molecularly imprinted polymer specially designed for Coenzyme Q10 (CoQ10 ) extraction was employed as a model to test this template elimination approach...
March 1, 2024: Journal of Chromatography. A
https://read.qxmd.com/read/38490412/dynamic-alterations-in-physiological-and-biochemical-indicators-of-cirrhinus-mrigala-hatchlings-a-sublethal-exposure-of-triclosan
#2
JOURNAL ARTICLE
Owias Iqbal Dar, Annadurai Vinothkanna, Bisma Aslam, Arajmand Furkh, Sunil Sharma, Arvinder Kaur, Yan-An Gao, Ai-Qun Jia
Triclosan (TCS), a biocide used in various day-to-day products, has been associated with several toxic effects in aquatic organisms. In the present study, biochemical and hematological alterations were evaluated after 14 d (sublethal) exposure of tap water (control), acetone (solvent control), 5, 10, 20, and 50 μg/L (environmentally relevant concentrations) TCS to the embryos/hatchlings of Cirrhinus mrigala, a major freshwater carp distributed in tropic and sub-tropical areas of Asia. A concentration-dependent increase in the content of urea and protein carbonyl, while a decrease in the total protein, glucose, cholesterol, triglycerides, uric acid, and bilirubin was observed after the exposure...
March 13, 2024: Science of the Total Environment
https://read.qxmd.com/read/38490144/determination-of-lipophilic-marine-biotoxins-azaspiracids-brevetoxins-and-okadaic-acid-group-and-domoic-acid-in-mussels-by-solid-phase-extraction-and-reversed-phase-liquid-chromatography-with-tandem-mass-spectrometry
#3
JOURNAL ARTICLE
Naoki Ochi, Toshiyuki Suzuki
An accurate and efficient method was developed for the determination of azaspiracid shellfish toxins (azaspiracids-1, -2, and -3), neurotoxic shellfish toxins (brevetoxins-2 and -3), diarrhetic shellfish toxins (okadaic acid and dinophysistoxins-1 and -2), and the amnesic shellfish toxin (domoic acid) in mussels (Mytilus galloprovincialis). Lipophilic marine biotoxins (azaspiracids, brevetoxins, and okadaic acid group) were extracted with 0.5 % acetic acid in methanol under heating at 60°C to improve the extraction efficiency of okadaic acid group toxins and then cleaned up with a C18 solid-phase extraction cartridge...
March 5, 2024: Journal of Chromatography. A
https://read.qxmd.com/read/38490143/a-nitrogenous-heterocyclic-ring-bonded-stationary-phase-for-separating-alkaloids-in-supercritical-fluid-chromatography
#4
JOURNAL ARTICLE
Chunying Song, Gaowa Jin, Dongping Yu, Zhimou Guo, Xinmiao Liang
A novel silica stationary phase was designed and prepared through thiol-epoxy click chemistry for supercritical fluid chromatography (SFC). The developed stationary phase was characterized by elemental analysis, Fourier transform infrared spectrometry and solid-state 13 C/CP MAS NMR spectroscopy. In order to evaluate the chromatographic performance and retention mechanisms of the prepared column, a variety of alkaloids were used, including indoles, isoquinolines, pyrrolidines, piperidines, quinolizidines and organic amines...
March 11, 2024: Journal of Chromatography. A
https://read.qxmd.com/read/38490142/a-functionalized-glass-fiber-as-the-adsorbent-for-efficient-analysis-of-endocrine-disruptors-in-aqueous-environments
#5
JOURNAL ARTICLE
Yingying Wang, Zhenyu Lu, Yayan Tong, Run Sun, Xue Liu, Na Chen, Mingxia Zhang, Yijun Zhang, Yuping Zhang
Estrogens and bisphenols are typical endocrine disruptors (EDs) that pose a potential hazard to the human body due to their widespread presence in aqueous environments. In this study, a β-cyclodextrin porous crosslinked polymer (β-CD-PCP) was prepared in-situ on a glass fiber surface by a nucleophilic substitution reaction. An effective and sensitive solid phase microextraction method using functionalized glass fiber with β-CD-PCP coating as the adsorbent was established for the detection of 11 EDs in a water environment...
March 11, 2024: Journal of Chromatography. A
https://read.qxmd.com/read/38490141/magnetic-solid-phase-extraction-based-on-restricted-access-molecularly-imprinted-polymers-for-ultrarapid-determination-of-ractopamine-residues-from-food-samples-by-capillary-electrophoresis
#6
JOURNAL ARTICLE
Daniel Ferreira Vidal, Bruna Carneiro Pires, Marcella Matos Cordeiro Borges, Hanna Leijoto de Oliveira, Camilla Fonseca Silva, Keyller Bastos Borges
An ultrafast, efficient, and eco-friendly method combining magnetic solid phase extraction and capillary electrophoresis with diode array detection have been developed to determine ractopamine residues in food samples. A restricted access material based on magnetic and mesoporous molecularly imprinted polymer has been properly synthesized and characterized, demonstrating excellent selectivity and high adsorbent capacity. Short-end injection capillary electrophoresis method was optimized: 75 mM triethylamine pH 7 as BGE, -20 kV, 50 mbar by hydrodynamic injection during 8 s, and capillary temperature at 25 °C; reaching ultrafast ractopamine analysis (∼0...
March 10, 2024: Journal of Chromatography. A
https://read.qxmd.com/read/38490140/magnetic-polyimide-nanocomposite-for-analysis-of-parabens-in-cooking-wine-by-magnetic-solid-phase-extraction-coupled-with-gas-chromatography-mass-spectrometry
#7
JOURNAL ARTICLE
Liuxin Wang, Xiaohui Yan, Xianzhong Chen, Yanshuo Li, Dapeng Wu
A magnetic polyimide (PI) nanocomposite has been synthesized by phase inversion of PI and simultaneous encapsulation of Fe3 O4 nanoparticles. The Fe3 O4 /PI nanocomposite was characterized by a variety of characterization techniques, including infrared spectroscopy, scanning electron microscopy, transmission electron microscopy, nitrogen adsorption-desorption isotherms, and vibrating sample magnetometry. The results showed that the prepared nanocomposite had a homogeneous structure, adequate specific surface area (76...
March 12, 2024: Journal of Chromatography. A
https://read.qxmd.com/read/38490042/liquid-chromatography-coupled-to-tandem-mass-spectrometry-lc-ms-ms-methods-for-the-therapeutic-drug-monitoring-of-cytotoxic-anticancer-drugs-an-update
#8
REVIEW
M Briki, A Murisier, M Guidi, C Seydoux, T Buclin, C Marzolini, F R Girardin, Y Thoma, S Carrara, E Choong, L A Decosterd
In the era of precision medicine, there is increasing evidence that conventional cytotoxic agents may be suitable candidates for therapeutic drug monitoring (TDM)- guided drug dosage adjustments and patient's tailored personalization of non-selective chemotherapies. To that end, many liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) assays have been developed for the quantification of conventional cytotoxic anticancer chemotherapies, that have been comprehensively and critically reviewed...
February 3, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38489940/exploring-the-potential-of-using-ion-mobility-mass-spectrometry-to-separate-matrix-interferences-from-analytes-in-food-control
#9
JOURNAL ARTICLE
Sjors Rasker, Marco H Blokland, Toine F H Bovee, Ane Arrizabalaga-Larrañaga
During residue analysis in complex matrices for food safety purposes, interfering signals can sometimes overlap with those of the analyte of interest. Access to an additional separation dimension besides chromatographic and mass separation, such as ion mobility, can aid in removing interfering signals, allowing for correct analyte identification in these cases. In our laboratory, during routine LC-MS/MS analysis of liver samples for growth promoter residues, an interfering signal was found that matches the retention time and m/z values for stanozolol, a synthetic anabolic steroid...
March 11, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38489939/a-quantitative-lc-ms-ms-analysis-of-xylazine-p-fluorofentanyl-fentanyl-and-fentanyl-related-compounds-in-postmortem-blood
#10
JOURNAL ARTICLE
Michael T Truver, Sara C Brogan, Emma A Jaeschke, Amy M Kinsey, Jennifer L Hoyer, Chris W Chronister, Michele M Crosby, Bruce A Goldberger
The purpose of this study was to develop and validate a method to quantitate the veterinary sedative xylazine as well as 4-anilino-N-phenethylpiperidine (4-ANPP), acetyl fentanyl, fentanyl, norfentanyl, and p-fluorofentanyl in blood utilizing liquid chromatography tandem mass spectrometry. This method also qualitatively monitors for the presence of o-fluorofentanyl and m-fluorofentanyl isomers. UCT Clean Screen® DAU extraction columns were utilized to isolate the analytes in postmortem blood samples. The extracts were eluted, evaporated, reconstituted, and then analyzed using a Waters Acquity™ UPLC coupled a triple quadrupole mass spectrometer...
February 28, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38488956/a-potential-involvement-of-metallothionein-in-the-zinc-tolerance-of-trichoderma-harzianum-experimental-findings
#11
JOURNAL ARTICLE
Negin Ejmalian, Soheila Mirzaei, Asghar Mirzaie-Asl, Mehrdad Chaichi
Metallothioneins are a group of cysteine-rich proteins that play an important role in the homeostasis and detoxification of heavy metals. The objective of this research was to explore the significance of metallothionein in Trichoderma harzianum tolerance to zinc. At the inhibitory concentration of 1000 ppm, the fungus adsorbed 16.7 ± 0.4 mg/g of metal. The HPLC and SDS-PAGE electrophoresis data suggested that the fungus production of metallothionein was twice as high in the presence of zinc as in the control group...
March 15, 2024: Protein Journal
https://read.qxmd.com/read/38488942/indirect-interactions-involving-the-psbm-or-psbt-subunits-and-the-psbo-psbu-and-psbv-proteins-stabilize-assembly-and-activity-of-photosystem-ii-in-synechocystis-sp-pcc-6803
#12
JOURNAL ARTICLE
Faiza Arshad, Julian J Eaton-Rye
The low-molecular-weight PsbM and PsbT proteins of Photosystem II (PS II) are both located at the monomer-monomer interface of the mature PS II dimer. Since the extrinsic proteins are associated with the final step of assembly of an active PS II monomer and, in the case of PsbO, are known to impact the stability of the PS II dimer, we have investigated the potential cooperativity between the PsbM and PsbT subunits and the PsbO, PsbU and PsbV extrinsic proteins. Blue-native polyacrylamide electrophoresis and western blotting detected stable PS II monomers in the ∆PsbM:∆PsbO and ∆PsbT:∆PsbO mutants that retained sufficient oxygen-evolving activity to support reduced photoautotrophic growth...
March 15, 2024: Photosynthesis Research
https://read.qxmd.com/read/38488745/strategies-to-deal-with-genetic-analyzer-specific-dna-methylation-measurements
#13
JOURNAL ARTICLE
Moon Hyun So, Ji Eun Lee, Hwan Young Lee
Targeted bisulfite sequencing using single-base extension (SBE) can be used to measure DNA methylation via capillary electrophoresis on genetic analyzers in forensic labs. Several accurate age prediction models have been reported using this method. However, using different genetic analyzers with different software settings can generate different methylation values, leading to significant errors in age prediction. To address this issue, the study proposes and compares four methods as follows: (1) adjusting methylation values using numerous actual body fluid DNA samples, (2) adjusting methylation values using control DNAs with varying methylation ratios, (3) constructing new age prediction models for each genetic analyzer type, and (4) constructing new age prediction models that could be applied to all types of genetic analyzers...
March 15, 2024: Electrophoresis
https://read.qxmd.com/read/38488707/a-dual-reference-study-design-for-understanding-and-improving-aav-genome-size-analysis
#14
JOURNAL ARTICLE
Yali Sun, Zhi-Xiang Lu, Michael Miller, Ying Valcour, Anis H Khimani, Jana Bauer, Michael Salomon, Yanhong Tong
Recombinant adeno-associated virus (rAAV) is the leading platform of gene delivery for its long-lasting gene transformation and low immunogenicity. Characterization of the integrity and purity of the rAAV genome is critical to ensure clinical potency and safety. However, current rAAV genome characterization methods that can provide size assessment are either time-consuming or not easily accessible to general labs. Additionally, there is a lack of right reference standard for analyzing long single-stranded DNA (ssDNA) fragments...
March 15, 2024: Electrophoresis
https://read.qxmd.com/read/38485631/interplay-of-electrokinetic-effects-in-nonpolar-solvents-for-electronic-paper-displays
#15
JOURNAL ARTICLE
Mohammad Khorsand Ahmadi, Wei Liu, Jan Groenewold, Jaap M J den Toonder, Alex Henzen, Hans M Wyss
HYPOTHESIS: Electronic paper displays rely on electrokinetic effects in nonpolar solvents to drive the displacement of colloidal particles within a fluidic cell. While Electrophoresis (EP) is a well-established and frequently employed phenomenon, electro-osmosis (EO), which drives fluid flow along charged solid surfaces, has not been studied as extensively. We hypothesize that by exploiting the interplay between these effects, an enhanced particle transport can be achieved. EXPERIMENTS: In this study, we experimentally investigate the combined effects of EP and EO for colloidal particles in non-polar solvents, driven by an electric field...
March 7, 2024: Journal of Colloid and Interface Science
https://read.qxmd.com/read/38484958/phenotypic-and-molecular-characterization-of-multi-drug-resistant-klebsiella-spp-isolates-recovered-from-clinical-settings
#16
JOURNAL ARTICLE
Hassan Diab, Kelven Rahy, Tamima Jisr, Mira El Chaar, Edmond Abboud, Sima Tokajian
Klebsiella pneumoniae is a Gram-negative bacterium that colonizes the gastrointestinal tract and nasopharynx with many being linked to nosocomial infections. Extended-spectrum β-lactamases (ESBL)-producing and carbapenem-resistant K. pneumoniae is recognized by the World Health Organization (WHO) as a critical public health concern. In this study, whole-genome sequencing (WGS) - based analysis was performed to understand the molecular epidemiology of multi-drug resistant Klebsiella spp. clinical isolates...
March 12, 2024: Infection, Genetics and Evolution
https://read.qxmd.com/read/38484676/pro-inflammatory-enzyme-inhibition-of-lipoxygenases-by-flavonoid-rich-extract-from-artemisia-vulgaris
#17
JOURNAL ARTICLE
Jin Ru Lim, Lee Suan Chua, Azizul Azri Mustaffa
The peroxyl radicals generated by the activity of lipoxygenases (LOX) are mediators to trigger inflammatory diseases. Therefore, it is important to investigate potent LOX inhibitor for modulating the occurrence and resolving inflammatory processes. Artemisa vulgaris, is a herbal plant that is known for flavonoids, potentially inhibiting lipid peroxidation and scavenging radicals. The objectives of the present study were to obtain flavonoids rich extract from A. vulgaris, and determine the inhibitory mode of the extract against LOX...
March 10, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38484675/sensitive-reliable-and-simultaneous-determination-of-fingolimod-and-citalopram-drug-molecules-used-in-multiple-sclerosis-treatment-based-on-magnetic-solid-phase-extraction-and-hplc-pda
#18
JOURNAL ARTICLE
Esra Durgun, Halil İbrahim Ulusoy, İbrahim Narin
An analytical methodology has been developed for trace amounts of Fingolimod (FIN) and Citalopram (CIT) drug molecules based on magnetic solid phase extraction (MSPE) and high performance liquid chromatographic determination with photodiode array detector (HPLC-DAD). Fingolimod is used in treatment of Multiple sclerosis (MS) disease and sometimes antidepressant drugs such as citalopram accompany to treatment. Both simultaneous analysis of these molecules and application of MSPE with a new adsorbent has been performed for first times...
March 11, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38484674/updates-of-the-current-strategies-of-labeling-for-n-glycan-analysis
#19
REVIEW
Yosra Helali, Cédric Delporte
This mini review summarizes the current methods used for screening N-glycosylation of glycoproteins, with a specific focus on therapeutic proteins and on techniques involving the release of N-glycans. With the continuous development of biopharmaceuticals, particularly monoclonal antibodies (mAbs), which are N-glycosylated proteins, monitoring has gained importance in recent decades. Glycosylation of therapeutic glycoproteins is considered a critical quality attribute because it can impact the efficacy and safety of these therapeutic drugs...
February 27, 2024: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://read.qxmd.com/read/38484640/controlled-synthesis-of-sulfhydryl-dendritic-mesoporous-silica-nanospheres-for-ultrafast-extraction-and-sensitive-analysis-of-organochlorine-herbicides-containing-amide-groups
#20
JOURNAL ARTICLE
Rui Shi, Yipeng Huang, Guihua Ruan, Zhengyi Chen, Yanqun Yang, Zhuqiang Wu
The distinctive morphology of dendritic mesoporous silica nanoparticles (DMSN) has recently attracted considerable attention in scientific community. However, synthesis of DMSN with well-defined structure and uniform size for ultrafast extraction of trace herbicide residues from environmental and food samples remains to be a compelling challenge. In this study, sulfhydryl functionalized dendritic mesoporous silica (SH-DMSN) was synthesized and the SH-DMSN showcases monodisperse microspheres with flower shape and precisely tailored and controllable pore sizes...
March 5, 2024: Journal of Chromatography. A
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