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Electrophoresis

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https://www.readbyqxmd.com/read/30106588/identification-of-missing-proteins-in-normal-human-cerebrospinal-fluid
#1
Charlotte Macron, Lydie Lane, Antonio Núñez Galindo, Loïc Dayon
The cerebrospinal fluid (CSF) proteome dataset presented herein was obtained after immunodepletion of abundant proteins and off-gel electrophoresis fractionation of a commercial pool of normal human CSF; liquid chromatography tandem mass spectrometry analysis was performed with a linear ion trap-Orbitrap Elite. We report the identification of 12344 peptides mapping on 2281 proteins. In the context of the Chromosome-Centric Human Proteome Project (C-HPP), the existence of seven missing proteins (MPs) is proposed to be validated...
August 14, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/30106436/decreasing-o-glcnacylation-affects-the-malignant-transformation-of-mcf-7-cells-via-hsp27-expression-and-its-o-glcnac-modification
#2
Pukkavadee Netsirisawan, Parunya Chaiyawat, Daranee Chokchaichamnankit, Kriengsak Lirdprapamongkol, Chantragan Srisomsap, Jisnuson Svasti, Voraratt Champattanachai
O-GlcNAcylation is a dynamic posttranslational modification of nucleoplasmic proteins. Previously, we reported that the O-GlcNAcylation level was increased in primary breast and colorectal cancer tissues. However, its precise roles in cancer development and progression are still largely unexplored. The aim of the present study was to investigate the roles of O-GlcNAcylation in the malignant transformation of cancer cell lines. O-GlcNAcylation level was examined in six cancer cell lines including breast (MCF-7 and MDA-MB-231), colorectal (SW480 and SW620), and liver (SK-Hep1 and HepG2)...
August 1, 2018: Oncology Reports
https://www.readbyqxmd.com/read/30106413/epidemiological-evaluation-of-an-acinetobacter-baumannii-outbreak-observed-at-an-intensive-care-unit
#3
Tuğba K Atik, Bülent Atik, Osman Kilinç, Bayhan Bektöre, Hülya Duran, Burak M Selek, Nihan Ceken, Orhan Baylan, Mustafa Özyurt
OBJECTIVES: To reveal the relationship between clinical and environmental isolates, analyzing both phenotypic and molecular aspects, in an Acinetobacter baumannii (A. baumannii) epidemic, and to use the epidemiological data to determine the source of the epidemic, to identify potential risk factors, and inform the effort to prevent and manage future epidemics. METHODS: Acinetobacter baumannii was isolated from 5 clinical samples in Sultan Abdulhamid Han Training and Research hospital, Istanbul, Turkey, for a week period...
August 2018: Saudi Medical Journal
https://www.readbyqxmd.com/read/30105450/fluoroquinolone-resistant-achromobacter-xylosoxidans-clinical-isolates-from-serbia-high-prevalence-of-the-aac-6-ib-cr-gene-among-resistant-isolates
#4
Branislav Lilić, Brankica Filipić, Milka Malešević, Katarina Novović, Zorica Vasiljević, Milan Kojić, Branko Jovčić
The aim of this study was to evaluate the contribution of plasmid-mediated genes and efflux to fluoroquinolone resistance in collection of Achromobacter spp. gathered during a 3-year period. Susceptibility to ciprofloxacin and levofloxacin was tested by disk diffusion and microdilution tests for a collection of 98 Achromobacter spp. clinical isolates. Identification of fluoroquinolone-resistant isolates was performed by sequencing and phylogenetic analyses of the nrdA gene. Genetic relatedness among resistant isolates was determined by pulsed-field gel electrophoresis (PFGE) analysis...
August 13, 2018: Folia Microbiologica
https://www.readbyqxmd.com/read/30105046/characterization-of-a-l-gulono-1-4-lactone-oxidase-like-protein-in-the-floral-nectar-of-mucuna-sempervirens-fabaceae
#5
Hong-Xia Zhou, Richard I Milne, Xue-Long Ma, Yue-Qin Song, Jian-Yu Fang, Hang Sun, Hong-Guang Zha
Floral nectar plays important roles in the interaction between animal-pollinated plants and pollinators. Its components include water, sugars, amino acids, vitamins, and proteins. Growing empirical evidence shows that most of the proteins secreted in nectar (nectarines) are enzymes that can tailor nectar chemistry for their animal mutualists or reduce the growth of microorganisms in nectar. However, to date, the function of many nectarines remains unknown, and very few plant species have had their nectar proteome thoroughly investigated...
2018: Frontiers in Plant Science
https://www.readbyqxmd.com/read/30104643/plasma-metabolomic-analysis-in-mature-female-common-bottlenose-dolphins-profiling-the-characteristics-of-metabolites-after-overnight-fasting-by-comparison-with-data-in-beagle-dogs
#6
Miwa Suzuki, Motoi Yoshioka, Yoshito Ohno, Yuichiro Akune
The present study was aimed at determining the characteristics of plasma metabolites in bottlenose dolphins to provide a greater understanding of their metabolism and to obtain information for the health management of cetaceans. Capillary electrophoresis-time-of-flight mass spectrometry (CE-TOFMS) and liquid chromatograph-time-of-flight mass spectrometry (LC-TOFMS) were conducted on plasma samples after overnight fasting from three common bottlenose dolphins as well as three beagle dogs (representative terrestrial carnivores) for comparison...
August 13, 2018: Scientific Reports
https://www.readbyqxmd.com/read/30104002/association-of-er-%C3%AE-gene-pvuii-polymorphism-with-ovarian-cancer
#7
Sirisha Pemmaraju, Lingaiah Amidyala, Ravindra Vottery, Pratibha Nallari, Jyothy Akka, Venkateshwari Ananthapur
BACKGROUND: Ovarian cancer is the most common cancer among women worldwide. Estrogen plays an important role in follicle formation and maturation of oocyte via its receptor (ER). It has a special interest as their protein levels are always elevated in premalignant and malignant cancer cells and are over expressed in different tumors with a favourable prognosis. The present study is aimed to evaluate the role of ER-α gene ( rs2234693) PVUII polymorphism in the etiology of ovarian cancer...
2018: Cancer Treatment and Research Communications
https://www.readbyqxmd.com/read/30103863/automated-techniques-in-pk-a-determination-low-medium-and-high-throughput-screening-methods
#8
REVIEW
Christophe Dardonville
Drug discovery programs that generate hundreds of new molecular entities need efficient methodologies for physicochemical profiling. Several high-throughput methods for pKa screening have been developed in the last 15 years to determine this key physicochemical parameter. Separation techniques such as HPLC-MS or capillary electrophoresis are particularly well-suited due to their high throughput and capacity to deal with impure or complex samples. In addition, potentiometric and (mostly) UV-metric-based methods (plate-based and automated systems), find their place as very precise methodologies for pKa determination despite of somewhat lower throughput...
July 2018: Drug Discovery Today. Technologies
https://www.readbyqxmd.com/read/30103779/comparative-1d-blue-native-electrophoresis-analysis-of-plasmodium-falciparum-and-human-proteins-associated-with-cytoadherence
#9
Yang Wu, Simon C Wagstaff, Saeed A Al-Harthi, Alister G Craig
BACKGROUND: To understand more about changes to the molecular components that occur when host endothelium interacts with Plasmodium falciparum-infected erythrocytes, a combined technique of protein separation (1D Blue-Native electrophoresis) and mass spectrometry of infected erythrocytes with endothelial cells (EC) in a co-culture system has been used. METHODS: Native proteins were extracted from co-cultures and identified by mass spectrometry. Proteomic data from different parasite strains, either adhesion proficient (to endothelial cells) or non-adherent, were analysed in parallel to reveal protein associations linked to cytoadherence...
August 13, 2018: Malaria Journal
https://www.readbyqxmd.com/read/30103694/leukotoxin-and-pyrogenic-toxin-superantigen-gene-backgrounds-in-bloodstream-and-wound-staphylococcus-aureus-isolates-from-eastern-region-of-china
#10
Chunyan He, Su Xu, Huanqiang Zhao, Fupin Hu, Xiaogang Xu, Shu Jin, Han Yang, Fang Gong, Qingzhong Liu
BACKGROUND: The bicomponent leukotoxins and the pyrogenic toxin superantigens (PTSAgs) are important virulence factors of Staphylococcus aureus. It is necessary to survey the prevalence and expression of these toxin-encoding genes for understanding the possible pathogenic capacity of S. aureus to cause disease. METHODS: Five leukotoxin genes and thirteen PTSAg determinants were detected for 177 S. aureus isolates from blood (n = 88) and wound (n = 89) infections by Polymerase Chain Reaction (PCR)...
August 13, 2018: BMC Infectious Diseases
https://www.readbyqxmd.com/read/30103671/bifunctionally-engineered-polyethylenimines-as-efficient-dna-carriers-and-antibacterials-against-resistant-pathogens
#11
Z Ahmadi, D Jha, B Kumar, H K Gautam, Pradeep Kumar
In this study, we have designed and developed two series of bifunctional conjugates by tethering polyethylenimine with streptomycin. By varying the amount of streptomycin, conjugates, polyethylenimine-streptomycin, have been synthesized and characterized spectroscopically. Gel electrophoresis assay revealed a slight decrease in the cationic charge density on the conjugates as these retarded the mobility of pDNA at higher w/w ratios. Further, transfection studies showed that both the series of conjugates transfected the mammalian cells efficiently with low-molecular weight polyethylenimine-streptomycin conjugates were more competent (∼9-fold enhancement with respect to native bPEI) exhibiting high cell viability too...
August 13, 2018: Journal of Biomaterials Applications
https://www.readbyqxmd.com/read/30103319/postsynaptic-proteome-of-non-demented-individuals-with-alzheimer-s-disease-neuropathology
#12
Olga Zolochevska, Nicole Bjorklund, Randall Woltjer, John E Wiktorowicz, Giulio Taglialatela
Some individuals, here referred to as Non-Demented with Alzheimer's Neuropathology (NDAN), retain their cognitive function despite the presence of amyloid plaques and tau tangles typical of symptomatic Alzheimer's disease (AD). In NDAN, unlike AD, toxic amyloid-β oligomers do not localize to the postsynaptic densities (PSDs). Synaptic resistance to amyloid-β in NDAN may thus enable these individuals to remain cognitively intact despite the AD-like pathology. The mechanism(s) responsible for this resistance remains unresolved and understanding such protective biological processes could reveal novel targets for the development of effective treatments for AD...
July 30, 2018: Journal of Alzheimer's Disease: JAD
https://www.readbyqxmd.com/read/30103295/modification-of-erythropoietin-structure-by-n-homocysteinylation-affects-its-antiapoptotic-and-proliferative-functions
#13
Agustina Schiappacasse, Romina Eugenia Maltaneri, María Eugenia Chamorro, Alcira Beatriz Nesse, Diana Elena Wetzler, Daniela Cecilia Vittori
Many patients under therapy with recombinant human erythropoietin (rhuEPO) show resistance to the treatment, an effect likely associated with the accumulation of tissue factors, especially in renal and cardiovascular diseases. Hyperhomocysteinemia due to high serum levels of homocysteine has been suggested among the risk factors in those pathologies. Its main effect is the N-homocysteinylation of proteins due to the interaction between the highly reactive homocysteine thiolactone (HTL) and lysine residues. The aim of this study was to evaluate the effect of N-homocysteinylation on the erythropoietic and antiapoptotic abilities of EPO, which can be a consequence of structural changes in the modified protein...
August 13, 2018: FEBS Journal
https://www.readbyqxmd.com/read/30102926/proteomic-analysis-of-2-monochloropropanediol-2-mcpd-and-2-mcpd-dipalmitate-toxicity-in-rat-kidney-and-liver-in-a-28-days-study
#14
Falko Frenzel, Axel Oberemm, Albert Braeuning, Alfonso Lampen
2-monochloropropanediol (2-MCPD), 3-monochloropropanediol (3-MCPD) and their fatty acid esters have recently been identified as heat-induced contaminants in fat- and salt-containing foodstuff. Toxicity of 3-MCPD has been studied previously in some detail. Disturbance of glycolysis and cellular redox functions appear to be involved in 3-MCPD toxicity. By contrast, only very few toxicological data are available for 2-MCPD or 2-MCPD esters, especially at the molecular level. This study was therefore aimed to provide a comprehensive overview of proteomic alterations induced in rat kidney and liver by 2-MCPD and 2-MCPD dipalmitate, a representative 2-MCPD fatty acid ester...
August 10, 2018: Food and Chemical Toxicology
https://www.readbyqxmd.com/read/30102709/electrical-discharges-in-water-induce-spores-dna-damage
#15
Camille Lamarche, Charlotte Da Silva, Gauthier Demol, Etienne Dague, Marie-Pierre Rols, Flavien Pillet
Bacterial spores are one of the most resilient life forms on earth and are involved in many human diseases, such as infectious diarrhea, fatal paralytic illnesses and respiratory infections. Here, we investigated the mechanisms involved in the death of Bacillus pumilus spores after exposure to electric arcs in water. Cutting-edge microscopies at the nanoscale did not reveal any structural disorganization of spores exposed to electric arcs. This result suggested the absence of physical destruction by a propagating shock wave or an exposure to an electric field...
2018: PloS One
https://www.readbyqxmd.com/read/30102516/microscale-reversed-phase-liquid-chromatography-capillary-zone-electrophoresis-tandem-mass-spectrometry-for-deep-and-highly-sensitive-bottom-up-proteomics-identification-of-7500-proteins-with-five-micrograms-of-an-mcf7-proteome-digest
#16
Zhichang Yang, Xiaojing Shen, Daoyang Chen, Liangliang Sun
Capillary zone electrophoresis-tandem mass spectrometry (CZE-MS/MS) has been well recognized for bottom-up proteomics. It has approached 4000-8000 protein identifications (IDs) from a human cell line, mouse brains or Xenopus embryos via coupling with liquid chromatography (LC) prefractionation. However, at least five hundred micrograms of complex proteome digests were required for the LC-CZE-MS/MS studies. This requirement of a large amount of initial peptide material impedes the application of CZE-MS/MS for deep bottom-up proteomics of mass-limited samples...
August 13, 2018: Analytical Chemistry
https://www.readbyqxmd.com/read/30102287/studying-ribonucleotide-incorporation-strand-specific-detection-of-ribonucleotides-in-the-yeast-genome-and-measuring-ribonucleotide-induced-mutagenesis
#17
Zhi-Xiong Zhou, Jessica S Williams, Thomas A Kunkel
The presence of ribonucleotides in nuclear DNA has been shown to be a source of genomic instability. The extent of ribonucleotide incorporation can be assessed by alkaline hydrolysis and gel electrophoresis as RNA is highly susceptible to hydrolysis in alkaline conditions. This, in combination with Southern blot analysis can be used to determine the location and strand into which the ribonucleotides have been incorporated. However, this procedure is only semi-quantitative and may not be sensitive enough to detect small changes in ribonucleotide content, although strand-specific Southern blot probing improves the sensitivity...
July 26, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/30101988/authors-reply-to-the-commentary-in-the-journal-of-electrophoresis-regarding-the-effect-of-simulated-space-radiation-on-the-n-glycosylation-of-human-immunoglobulin-g1-by-j-j-bevelacqua-and-s-m-j-mortazavi
#18
Andras Guttman, Szabolcs Szilasi, Boglarka Donczo, Mate Szarka
No abstract text is available yet for this article.
August 13, 2018: Electrophoresis
https://www.readbyqxmd.com/read/30101939/a-case-of-lambda-light-chain-non-crystalline-proximal-tubulopathy-with-igd-lambda-myeloma
#19
Seiji Kishi, Fumiaki Obata, Hirokazu Miki, Motokazu Matsuura, Kenji Nishimura, Masanori Tamaki, Fumi Kishi, Taichi Murakami, Hideharu Abe, Kojiro Nagai, Masahiro Abe, Toshio Doi
Light Chain Proximal Tubulopathy (LCPT) is a rare form of paraprotein-related kidney disease in which monoclonal free light chains damage the proximal renal tubular epithelial cells. We herein report the case of a 78-year-old woman who presented with anemia and kidney dysfunction. Serum and urine protein electrophoresis analyses revealed a monoclonal IgD and λ free light chains. Proximal tubular injury and the accumulation of λ light chains were found by kidney biopsy. Electron microscopy revealed no organized structure suggestive of crystals...
August 10, 2018: Internal Medicine
https://www.readbyqxmd.com/read/30101884/evaluation-of-type-a-endonucleases-for-the-quantitative-analysis-of-dna-damage-due-to-exposure-to-acetaldehyde-using-capillary-electrophoresis
#20
Yukihiro Esaka, Kenji Hisato, Takuhei Yamamoto, Hiroya Murakami, Bunji Uno
The substrate selectivities of three endonucleases were studied quantitatively using capillary zone electrophoresis to find one giving N2 -ethyl(Et)-2'-deoxyguanosine-5'-monophosphate (5'-dGMP) and cyclic 1,N2 -propano(CPr)-5'-dGMP from DNAs damaged by acetaldehyde (AA). Six 2'-deoxyribonucleoside-5'-monophosphates to be quantified in the hydrolysis solutions of DNAs, namely, Et-5'-dGMP, CPr-5'-dGMP, and four authentic ones, were completely separated using a 100 mM borate running buffer solution having an optimized pH of 9...
2018: Analytical Sciences: the International Journal of the Japan Society for Analytical Chemistry
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