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John J Kim, Peggy P Y Chan, Julea Vlassakis, Alisha Geldert, Amy E Herr
Immunoblotting confers protein identification specificity beyond that of immunoassays by prepending protein electrophoresis (sizing) to immunoprobing. To accurately size protein targets, sample analysis includes concurrent analysis of protein markers with known molecular masses. To incorporate protein markers in single-cell western blotting, microwells are used to isolate individual cells and protein marker-coated microparticles. A magnetic field directs protein-coated microparticles to >75% of microwells, so as to 1) deliver a quantum of protein marker to each cell-laden microwell and 2) synchronize protein marker solubilization with cell lysis...
October 17, 2018: Small
Asghar Farajzadeh, S Zahra Bathaie, Jalil Arabkheradmand, Seyed Mohammad Ghodsi, Soghrat Faghihzadeh
Trigeminal neuralgia (TN) with chronic and severe neuropathic pain leads to remarkable interference in daily living activities of patients. Unknown molecular mechanisms involved in TN pathophysiology are a challenge for complete treatment of the disease. The present study was conducted to investigate changes in the plasma proteome beside biochemical parameters, including calcitonin gene-related peptide (CGRP), nitric oxide (NO), amino acids, and vitamin D (Vit D) in different pain states in TN patients. Plasma samples were obtained from the control group (#13) and patients with purely paroxysmal type of classical TN (#13) before and after microvascular decompression (MVD)...
October 17, 2018: Journal of Molecular Neuroscience: MN
Bikash Kumar, Nisha Bhardwaj, Ansar Alam, Komal Agrawal, Himanshu Prasad, Pradeep Verma
A cellulase producing fungus Schizophyllum commune NAIMCC-F-03379 was isolated from decomposed leaf sample of Lantana camera. The nutritional components (wheat bran, magnesium sulphate and calcium chloride concentrations) and physical parameters (temperature and pH) were optimised by response surface methodology for enhanced cellulase production by S. commune NAIMCC-F-03379. The optimized medium contained: 1% (w/v) wheat bran, 0.3 g/L MgSO4, 0.8-1.0 g/L CaCl2 , optimum temperature and pH were 25 °C and 5 respectively...
October 17, 2018: AMB Express
Ali Mohammed Yousef, Sherry Kamel Abdelrahman, Ibrahim Elsayed Helmi, Fathy Mohammed Eltaweel, Walaa Elsayed Hamed
BACKGROUND: Autoantibodies are useful diagnostic tools for a variety of rheumatic autoimmune disorders. OBJECTIVE: The aim of the present study was to investigate autoantibodies against the 70-kD polypeptide of U1 ribonucleoprotein antibodies (RNP) in patients with rheumatoid arthritis (RA) and to investigate the relationship between anti-RNP and HLARD4 in RA patients. PATIENTS AND METHODS: In this prospective study, we studied 80 RA patients...
October 17, 2018: Journal of Immunoassay & Immunochemistry
Catherine H-W Hsu, Jonathan Langdown, Roderick Lynn, Chris Fisher, Anna Rose, Melanie Proven, Jennifer Eglinton, Martin W Besser
We report a novel hemoglobin (Hb) variant with a β chain amino acid substitution at codon 78 (CTG>CCG) (HBB: c.236T>C), detected through prenatal screening via capillary electrophoresis (CE) in an otherwise healthy and asymptomatic 38-year-old female of Southeast Asian ancestry. The variant, named Hb Penang after the proband's Malaysian city of origin, underwent further characterization through high performance liquid chromatography (HPLC), reversed phase HPLC, Sanger sequencing, isopropanol stability testing and isoelectric focusing (IEF)...
October 17, 2018: Hemoglobin
Gabriela Hernández-Silva, Marta Durand, Fernando Larrea, Mayel Chirinos
When levonorgestrel (LNG) is given for emergency contraception during the follicular phase, it not only inhibits or delays ovulation, but also induces changes in endometrial secretions that modulate sperm functionality. In order to characterize the female reproductive tract secreted molecules that may affect human spermatozoa, we analyzed changes in the protein content of uterine flushings obtained from women during the periovulatory phase of a control and a LNG-treated menstrual cycle. Lectin affinity analysis and 2D gel electrophoresis of uterine samples showed changes in protein glycosylation patterns and the presence of 31 differentially expressed proteins (8 upregulated and 23 downregulated)...
September 12, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
Muhammad Usman Akbar
Schiff base ligands are biologically active compounds in having antimicrobial, antiviral and antitumor activities etc. In this study, we have synthesized a Schiff base ligand namely 4-hydroxy-3-(2-hydroxy-3-methoxybenzylideneamino) benzoic acid, by reacting 3-amino-4-hydroxybenzoic acid and 2-hydroxy-3-methoxy benzaldehyde in the presence of acetic acid and refluxing it. The resulting base ligand was characterized on HPLC and used for radiolabelling with technetium-99m. The ligand 4-hydroxy-3-(2-hydroxy-3-methoxybenzylideneamino) benzoic acid was labelled with 99m Tc at pH 7, while reacting 230 µg of ligand with 15 mCi of 99m TcO- 4 for 10 min at room temperature...
October 16, 2018: Molecular Biology Reports
Jianming Zhang, Yanyan Yang, Hui Yang, Yushan Bu, Huaxi Yi, Lanwei Zhang, Xue Han, Lianzhong Ai
The exploration and evaluation of bacteriocin-producing lactic acid bacteria (LAB) have been one of the powerful means to food preservation. A total of 300 strains were isolated from Chinese traditional fermented milk products. A bacteriocin-producing LAB, named Lactobacillus plantarum J23, was screened and identified. Bacteriocin Lac-B23 from L. plantarum J23 was purified by 80% ammonium sulfate precipitation, cation-exchange chromatography, and reverse-phase high-performance liquid chromatography. Molecular weight of bacteriocin Lac-B23 was determined to be approximately 6...
2018: Frontiers in Microbiology
Corentin Berardet, Julia Kaffy, Sandrine Ongeri, Myriam Taverna
Type 2 diabetes is characterized by the aggregation of human Islet Amyloid Polypeptide (hIAPP) from monomer to large and insoluble fibrils. According to several recent studies, small soluble oligomers are now considered as potential toxic species. No monitoring tool has been to date reported to mimic in vitro the oligomerization process of hIAPP over time, although this would allow selecting candidate compounds that slow down or stop this pathological process. Considering the poor stability of those species and the necessity to monitor in real time, a compatible with the monitoring of hIAPP oligomerization CE method coupled to UV detection was developed...
October 9, 2018: Journal of Chromatography. A
Sutima Preeprem, Kamonnut Singkhamanan, Mitsuaki Nishibuchi, Varaporn Vuddhakul, Pimonsri Mittraparp-Arthorn
Pandemic O3:K6 Vibrio parahaemolyticus emerged in 1996. Since then, this strain of pathogen and its serovariants (predominantly O1:KUT [untypable], O1:K25 and O4:K68) have caused gastroenteritis worldwide. Owing to the limitation in established K antisera, tracking the sources of KUT for epidemiological investigation is difficult. Therefore, the effective molecular typing is required to discriminate the strains. The aim of this study was to develop a multiplex multilocus variable-number tandem-repeat analysis (MLVA) assay for typing pandemic V...
October 16, 2018: Foodborne Pathogens and Disease
Shigeyuki Mukudai, Nao Hiwatashi, Renjie Bing, Michael Garabedian, Ryan C Branski
OBJECTIVES/HYPOTHESIS: Direct glucocorticoid (GC) injection for vocal fold (VF) scarring has evolved as a therapeutic strategy, but the mechanisms underlying the antifibrotic effects remain unclear. GCs act via the glucocorticoid receptor (GR), which is phosphorylated at multiple serine residues in a hormone-dependent manner to affect bioactivity. We hypothesize that GCs regulate SMAD signaling via GR phosphorylation in vocal fold fibroblasts (VFFs). STUDY DESIGN: In vitro...
October 16, 2018: Laryngoscope
G A Raviraj, Kishore G Bhat, Manohar S Kugaji, Vijay M Kumbar, Amruta Hooli
Background: Recent investigations have shown the possible involvement of bacteria other than mutans group and Lactobacilli in the etiology of caries. Molecular methods have been used to study the microbial diversity in caries-active (CA) and caries-free (CF) children. Among them, denaturing gradient gel electrophoresis (DGGE) is more popular and has been used in the present study. Aims: The aim of the present study was to investigate the difference in bacterial diversity in saliva and plaque samples from CF and CA children using DGGE...
October 2018: Journal of the Indian Society of Pedodontics and Preventive Dentistry
Maryam Ranjpour, Saima Wajid, Swatantra Kumar Jain
Identification of events leading to HCC progression is essential for understanding its pathophysiology. Present study was designed to identify differentially expressed proteins in serum of HCC-bearing rats vis-a-vis controls during cancer initiation, progression and tumorigenesis. HCC was chemically induced by administering N-Nitrosodiethylamine and 2-aminoacetylfluorine to male Wistar rats. Carcinogen administration caused inflammation leading to liver injury and HCC development. Liver inflammation was confirmed by increase in levels of IL-6 and TNF-α in carcinogen treated rats...
October 15, 2018: Anti-cancer Agents in Medicinal Chemistry
Farzad Zehsaz, Hamed Abbasi Soltani, Roya Hazrati, Negin Farhangi, Amir Monfaredan, Mehri Ghahramani
The purpose of the research was to examine if some genetic variations are associated with some endurance, power and speed performances (multi-stage 20-m shuttle run, standing broad jump, 20 m sprint test and Abalakov jump) in a group of 586 non-trained male adolescents (mean ± SD age: 13.20 ± 0.25 years). Polymorphisms in PPARa and PPARGC1A implicated in physical performance traits were analyzed. DNA was extracted and the samples were genotyped for PPARa and PPARGC1A polymorphisms by a PCR based method followed by gel electrophoresis...
October 15, 2018: Molecular Biology Reports
Daisuke Todokoro, Hiroshi Eguchi, Takashi Suzuki, Motoo Suzuki, Haruyuki Nakayama-Imaohji, Tomomi Kuwahara, Takahiro Nomura, Haruyoshi Tomita, Hideo Akiyama
PURPOSE: Enterococcus faecalis causes severe acute endophthalmitis and often leads to poor visual outcomes. Conjunctival bacterial cultures occasionally grow atypical bacteria including E. faecalis, which can potentially contribute to the development of postoperative endophthalmitis. However, the characteristics of these ocular E. faecalis strains are unknown. This study is the first attempt to determine the population characteristics of E. faecalis clinical isolates from eye infections and ocular commensals...
October 15, 2018: Japanese Journal of Ophthalmology
Nasiri Sayed Mahdi, Farideh Azarbani, Afshin Pirnia, Abolfazl Abbaszadeh, Mohammadreza Gholami
Background: Cancer treatment methods can lead to male infertility .in this regard, cryopreservation of spermatogonial stem cells (SSC) and cell-to-person transplantation after the course of treatment to resolve the problem of infertility is a good one. The cryopreservation of SSC is an important process as it can help on the return of spermatogenesis. However, during this process, the stem cells often become damaged which degrades their value for experiments and treatments. Caffeic acid (CA) is an antioxidant that has been shown to increase the viability of cells under stress...
October 2018: Reports of Biochemistry & Molecular Biology
Louise Ladefoged Poulsen, Magne Bisgaard, Steffen Lynge Jørgensen, Tommy Dideriksen, Jacob Roland Pedersen, Henrik Christensen
The aim of the study was to investigate cellulitis caused by Escherichia coli which has been responsible for economic and welfare problems in Danish broiler production between 2014 and 2016. The study included 13 flocks with unusually high condemnation rates due to cellulitis during a period of approximately one year. From six flocks, 126 condemned carcasses were collected at a Danish slaughterhouse. Further 272 broilers dead on their own were collected on nine broiler farms from flocks with increased mortality and cellulitis (2 farms included both birds from the rearing period and broilers subsequently condemned)...
November 2018: Veterinary Microbiology
Huixian Tong, Juxiang Liu, Xiaohui Yao, Haiyan Jia, Jianchao Wei, Donghua Shao, Ke Liu, Yafeng Qiu, Zhiyong Ma, Beibei Li
The mcr-1 gene is a plasmid-borne and globally distributed colistin-resistance gene, which threatens the last-line role of colistin in treatment of infections caused by multi-drug resistant(MDR) Gram-negative bacteria in humans. The aim of this study was to obtain a comprehensive understanding of mcr-1 prevalence in the pig industry in China. A total of 600 faecal samples were collected from 60 swine farms in 18 provinces of China. Faecal DNA was extracted and subjected to PCR screening to detect the presence of mcr-1...
November 2018: Veterinary Microbiology
Marianne Dor, Simone Eperon, Patrice H Lalive, Yan Guex-Crosier, Mehrad Hamedani, Cindy Salvisberg, Natacha Turck
Considering absence of invasiveness and side effects, tears emerge as a particularly attractive fluid for biomarker discovery and therefore for daily clinical use. However, to date this fluid remains poorly studied in healthy condition. Here, we present an updated in-depth characterisation of the human healthy tear protein composition using proteomics approach. Both eyes of 8 healthy controls (4 men and 4 women, average age: 38 ± 18) were collected using the Schirmer's strip method. After liquid digestion and off-gel electrophoresis fractionation, three independent proteomics analyses were performed on a LTQ-Orbitrap Velos Pro...
October 12, 2018: Experimental Eye Research
Marcus Vinícius Cardoso Trento, Mateus William de Faria Eleutério, Tatiane Silva Abreu, Gustavo Henrique Andrade Machado, Pedro Henrique Souza Cesar, Anderson Assaid Simão, Silvana Marcussi
The objective of this study was to evaluate the genotoxic and mutagenic effects of the toxins present in Lachesis muta muta's venom on human peripheral blood leukocytes and the protective potential of ascorbic acid on DNA fragmentation. The venom of L. muta muta was incubated in different concentrations (1, 2.5, 5, 7.5, 10, 15, 20, 30, 40, 50, 60, and 120 µg/mL) with human blood to evaluate DNA fragmentation using the comet, agarose gel electrophoresis, and micronucleus assays. In these concentrations evaluated, the venom of L...
October 15, 2018: Journal of Cellular Biochemistry
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