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Shahid Hussain, Sonal Saxena, Sameer Shrivastava, Ashok Kumar Mohanty, Sudarshan Kumar, Rajkumar James Singh, Abhinav Kumar, Sajad Ahmad Wani, Ravi Kumar Gandham, Naveen Kumar, Anil Kumar Sharma, Ashok Kumar Tiwari, Raj Kumar Singh
Spontaneously occurring canine mammary tumours (CMTs) are the most common neoplasms of unspayed female dogs leading to thrice higher mortality rates than human breast cancer. These are also attractive models for human breast cancer studies owing to clinical and molecular similarities. Thus, they are important candidates for biomarker studies and understanding cancer pathobiology. The study was designed to explore underlying molecular networks and pathways in CMTs for deciphering new prognostic factors and therapeutic targets...
2018: PloS One
Cuiping Jiang, Zitong Qi, Hengbo Jia, Yilei Huang, Yunbo Wang, Wenli Zhang, Zimei Wu, Hu Yang, Jianping Liu
In this work, we report on an ATP-responsive low-molecular-weight polyethylenimine (LMW-PEI)-based supramolecular assembly. It formed via host-guest interaction between PEI (MW=1.8 kDa)-α-cyclodextrin (α-CD) conjugates and PEI1.8k-phenylboronic acid (PBA) conjugates. The host-guest interaction between PEI1.8k-α-CD and PEI1.8k-PBA was confirmed by the 2D-NOESY chromatogram experiment and competition test. The ATP-responsive property of the supramolecular assembly was evaluated by a series of ATP-triggered degradation and siRNA release studies in terms of fluorescence resonance energy transfer, agarose gel electrophoresis assay and the time course monitoring of the particle size and morphology...
December 5, 2018: Biomacromolecules
Yilan Ouyang, Xiaorui Han, Qiangwei Xia, Jianle Chen, Sheila Velagapudi, Ke Xia, Zhenqing Zhang, Robert J Linhardt
Glycosaminoglycans (GAGs) are biologically and pharmacologically important linear, anionic polysaccharides containing various repeating disaccharides sequences. The analysis of these polysaccharides generally relies on their chemical or enzymatic breakdown to disaccharide units that are separated, by chromatography or electrophoresis, and detected, by UV, fluorescence or mass spectrometry (MS). Isoelectric focusing (IEF) is an important analytical technique with high resolving power for the separation of analytes exhibiting differences in isoelectric points...
December 5, 2018: Analytical Chemistry
Jillian Rumore, Lorelee Tschetter, Ashley Kearney, Rima Kandar, Rachel McCormick, Matthew Walker, Christy-Lynn Peterson, Aleisha Reimer, Celine Nadon
BACKGROUND: Rapid and accurate identification of Verotoxigenic Escherichia coli (VTEC) O157:H7 is dependent on well-established, standardized and highly discriminatory typing methods. Currently, conventional subtyping tests for foodborne bacterial pathogen surveillance are rapidly being replaced with whole-genome sequencing (WGS) in public health laboratories. The capacity of WGS to revolutionize global foodborne disease surveillance has positioned this tool to become the new gold standard; however, to ensure evidence standards for public health decision making can still be achieved, the performance of WGS must be thoroughly validated against current gold standard methods prior to implementation...
December 4, 2018: BMC Genomics
Yi-Hsuan Tang, Sodio C N Hsu, Po-Yu Chen, Si-Ting Liou, Hui-Ting Chen, Carol Hsin-Yi Wu, Chai-Lin Kao
A family of bis (2-pyridyl)amino-modified poly(amidoamine) dendrimer Cu complexes was prepared, and their chemical nuclease activities and binding affinity ( Kb ) levels for DNA plasmid were investigated. The K b values of the G2 to G6 apodendrimers for DNA plasmid were found to be 7.4, 23, 48, 70, and 280 µM-1 , respectively, using ethidium bromide (EtBr) displacement experiments. The chemical nuclease activities of the corresponding complexes were determined by gel electrophoresis, and a clear positive dendritic effect was observed...
December 3, 2018: Pharmaceutics
Yuki Hanadate, Yumiko Saito-Nakano, Kumiko Nakada-Tsukui, Tomoyoshi Nozaki
Membrane traffic plays a pivotal role in virulence in the enteric protozoan parasite Entamoeba histolytica . EhRab8A small GTPase is a key regulator of membrane traffic at the endoplasmic reticulum (ER) of this protist and is involved in the transport of plasma membrane proteins. Here we identified the binding proteins of EhRab8A. The Cdc50 homolog, a non-catalytic subunit of lipid flippase, was identified as an EhRab8A binding protein candidate by affinity coimmunoprecipitation. Binding of EhRab8A to EhCdc50 was also confirmed by reciprocal immunoprecipitation and blue-native polyacrylamide gel electrophoresis, the latter of which revealed an 87 kDa complex...
November 30, 2018: International Journal of Molecular Sciences
Farideh Tabatabaei Yazdi, Abbas Tanhaeian, Marjan Azghandi, Alireza Vasiee, Behrooz Alizadeh Behbahani, Seyed Ali Mortazavi, Sahar Roshanak
The antimicrobial peptide Thrombocidin-1 (TC-1) isolated from human blood that derived from NAP-2 by deleting of two amino acids from C-terminal region. In this study, a C-terminal 6 _ His tagged recombinant TC-1 was expressed as a secreted peptide in Pichia pastoris, for the first time. The recombinant P. pastoris was inoculated in to BMMY culture medium, incubation with 5 μl/ml absolute methanol for 72 h at 30 °C. The TC-1 peptide was concentrated with nickel affinity chromatography and electrophoresis on 16% acrylamide gels...
December 1, 2018: Microbial Pathogenesis
Jin Zhao, Haixiong Xia, Yujie Liu, Pal Ravinder, Anran Fan, Zhixu He, Lingwen Zeng
OBJECTIVE: To investigate the rapid and accurate method for the detection of dengue virus (DENV) by using nicking enzyme assisted strand-displacement amplification (SDA) combined with gold nanoparticles-based lateral flow strip. METHODS: Total RNA of the virus was extracted by using magnetic beads method and transcribed to cDNA for SDA detection system. Nicking enzyme-assisted method was used for detecting DENV, and agarose gel electrophoresis was used for analyzing the sensitivity of SDA amplification products...
February 25, 2018: Zhejiang da Xue Xue Bao. Yi Xue Ban, Journal of Zhejiang University. Medical Sciences
Mohammad Asaduzzaman Miah, Mohammed Esmail Abdalla Elzaki, Asmaul Husna, Zhaojun Han
Deltamethrin resistance in Laodelphax striatellus had been associated with its oxidative detoxification by overexpression of four cytochrome P450 monooxygenases like CYP353D1v2, CYP6FU1, CYP6AY3v2, and CYP439A1v3. The first three P450s have been validated for insecticide-metabolizing capability and only CYP6FU1 was found to degrade deltamethrin. In this study, an investigation was conducted to confirm the capability of CYP439A1v3 to degrade deltamethrin. The CYP439A1v3 was first expressed in Sf9 cell line and its recombinant enzyme was tested for metabolic activity against different insecticides using substrate depletion assay combined with metabolite identification...
December 4, 2018: Archives of Insect Biochemistry and Physiology
Puthiya Veettil Vipindas, Thajudeen Jabir, Chekidhenkuzhiyil Jasmin, Tharakan Balu, Thekkendavida Velloth Rehitha, Balakrishnan Meenakshikutty Adarsh, Shanta Nair, Mohamed Hatha Abdulla, Anas Abdulaziz
Diversity and distribution pattern of ammonia-oxidizing archaea (AOA) were studied across a salinity gradient in the water column of Cochin Estuary (CE), a tropical monsoonal estuary along the southeast Arabian Sea. The water column of CE was found to be nutrient rich with high bacterial (3.7-6.7 × 108  cells L-1 ) and archaeal abundance (1.9-4.5 × 108  cells L-1 ). Diversity and seasonal variation in the distribution pattern of AOA were studied using clone library analysis and Denaturing gradient gel electrophoresis (DGGE)...
December 3, 2018: World Journal of Microbiology & Biotechnology
Pasquale Picone, Maria Antonietta Sabatino, Alessia Ajovalasit, Ornella Roberta Brancato, Daniela Giacomazza, Clelia Dispenza, Marta Di Carlo
In wound dressing applications, exudate absorption and retention are important properties. The data presented here assess the ability of the crosslinked xyloglucan-poly(vinyl alcohol) hydrogel films (XG-PVA), described in "Xyloglucan-based hydrogel films for wound dressing: Structure-property relationships" (Ajovalasit et al., 2018) [1] and "Biocompatibility, hemocompatibility and antimicrobial properties of xyloglucan-based hydrogel film for wound healing application" (Picone et al., 2019), to absorb and retain proteins...
December 2018: Data in Brief
Noha Attia, Mohamed Mashal, Cristina Soto-Sánchez, Gema Martínez-Navarrete, Eduardo Fernández, Santiago Grijalvo, Ramón Eritja, Gustavo Puras, Jose Luis Pedraz
Background: Gene therapy can be an intriguing therapeutic option in wide-ranging neurological disorders. Though nonviral gene carriers represent a safer delivery system to their viral counterparts, a thorough design of such vehicles is crucial to enhance their transfection properties. Purpose: This study evaluated the effects of combined use of two nonionic surfactants, poloxamer 188 (P) and polysorbate 80 (P80) into nanovesicles - based on 2,3-di(tetradecyloxy)propan-1-amine cationic lipid (D) - destined for gene delivery to central nervous system cells...
2018: Drug Design, Development and Therapy
Nelson Leung, Frank Bridoux, Vecihi Batuman, Aristeidis Chaidos, Paul Cockwell, Vivette D D'Agati, Angela Dispenzieri, Fernando C Fervenza, Jean-Paul Fermand, Simon Gibbs, Julian D Gillmore, Guillermo A Herrera, Arnaud Jaccard, Dragan Jevremovic, Efstathios Kastritis, Vishal Kukreti, Robert A Kyle, Helen J Lachmann, Christopher P Larsen, Heinz Ludwig, Glen S Markowitz, Giampaolo Merlini, Peter Mollee, Maria M Picken, Vincent S Rajkumar, Virginie Royal, Paul W Sanders, Sanjeev Sethi, Christopher P Venner, Peter M Voorhees, Ashutosh D Wechalekar, Brendan M Weiss, Samih H Nasr
The term monoclonal gammopathy of renal significance (MGRS) was introduced by the International Kidney and Monoclonal Gammopathy Research Group (IKMG) in 2012. The IKMG met in April 2017 to refine the definition of MGRS and to update the diagnostic criteria for MGRS-related diseases. Accordingly, in this Expert Consensus Document, the IKMG redefines MGRS as a clonal proliferative disorder that produces a nephrotoxic monoclonal immunoglobulin and does not meet previously defined haematological criteria for treatment of a specific malignancy...
December 3, 2018: Nature Reviews. Nephrology
Chen Wang, Chun Liang
Microsatellite instability (MSI) is characterized by high degree of polymorphism in microsatellite lengths due to deficiency in mismatch repair (MMR) system. MSI is associated with several tumor types and its status can be considered as an important indicator for patient prognosis. Conventional clinical diagnosis of MSI examines PCR products of a panel of microsatellite markers using electrophoresis (MSI-PCR), which is laborious, costly, and time consuming. We developed MSIpred, a python package for automatic MSI classification using a machine learning technology - support vector machine (SVM)...
December 3, 2018: Scientific Reports
Jennifer C Darnell, Aldo Mele, Ka Ying Sharon Hung, Robert B Darnell
This protocol describes the purification by denaturing polyacrylamide gel electrophoresis of RNA linkers for cross-linking immunoprecipitation (CLIP). Purification is necessary because if the 3' linker loses the puromycin blocking group, concatemerization of the 3' linker will occur during the 3' linker ligation reaction. In addition, truncated linkers make bioinformatic processing of the sequencing results more difficult than it need be. Additionally, this protocol describes the treatment of coimmunoprecipitated RNA tags for CLIP with alkaline phosphatase to remove the 3' phosphate remaining after RNase digestion...
December 3, 2018: Cold Spring Harbor Protocols
Shu Zhao, Licai Ma, Yuan Wang, Guihong Fu, Junfang Zhou, Xincang Li, Wenhong Fang
Environmental antimicrobial resistance (AMR) has drawn increasing attention due to its great risk to human health. The aim of this study was to investigate AMR and genotyping of Vibrio parahaemolyticus isolates (n = 114) recovered from shrimp mariculture environment in China. The isolates exhibited a high rate of resistance to streptomycin (78.9%), ampicillin (64.9%) and gentamicin (53.5%). Furthermore, multi-drug resistance was highly prevalent (61.4%), in which 95.9% of these ampicillin-resistant isolates were primarily mediated by blaCARB-17 ...
November 2018: Marine Pollution Bulletin
Zuzana Cieslarova, Mariana Magaldi, Lucélia Alcantara Barros, Claudimir Lucio do Lago, Daniel Rossado Oliveira, Francisco Antonio Helfenstein Fonseca, Maria Cristina Izar, Aline Soriano Lopes, Marina Franco Maggi Tavares, Aline Klassen
A capillary electrophoresis with diode array and tandem mass spectrometry detection (CE-UV-MS/MS) method has been developed for the targeted assessment of cardiovascular biomarkers candidates, trimethylamine-N-Oxide (TMAO) and l-carnitine, and creatinine in human urine samples. The dual detection was applied due to the high concentration of creatinine (monitored by UV detection at 200 nm) in relation to TMAO and l-carnitine (quantified by selected reaction monitoring (SRM) mass spectrometry), in human urine...
November 2, 2018: Journal of Chromatography. A
Hoang T Kim Hong, Truong T Bich Phuong, Nguyen T Thu Thuy, Matthew D Wheatley, John C Cushman
This paper presents a simultaneous isolation of pure, intact chloroplasts and mitochondria from mature leaves of Ice plant (Mesembryanthemum crystallinum) and mitochondrial protein preparation for two-dimensional electrophoresis (2DE) analysis under well watered and water -deficit stressed treatments. The washed chloroplasts and mitochondria were purified with Percoll gradients prepared using a Master flex R pump. The chloroplast and mitochondrial proteins were extracted in lysis buffer containing a protease inhibitor mix supplemented with 1 μM Leupeptin and 1 μM E64, followed by precipitation with ice-cold acetone...
November 30, 2018: Protein Expression and Purification
Alicia DiBattista, Nathan McIntosh, Monica Lamoureux, Osama Y Al-Dirbashi, Pranesh Chakraborty, Philip Britz-McKibbin
Cystic fibrosis (CF) is a complex multi-organ disorder that is among the most common fatal genetic diseases benefiting from therapeutic interventions early in life. Newborn screening (NBS) for pre-symptomatic detection of CF currently relies on a two-stage immunoreactive trypsinogen (IRT) and cystic fibrosis transmembrane conductance regulator (CFTR) mutation panel algorithm that is sensitive, but not specific for identifying affected neonates with a low positive predictive value. For the first time, we report the discovery of a panel of CF-specific metabolites from a single 3...
December 3, 2018: Journal of Proteome Research
Gotravalli V Rudresha, Amog P Urs, Vaddarahally N Manjuprasanna, Kanve N Suvilesh, Puttappa Sharanappa, Bannikuppe S Vishwanath
Echis carinatus envenomation leads to severe tissue necrosis at the bitten site by releasing DNA from immune cells that blocks the blood flow. An earlier report has shown that exogenous DNase 1 offers protection against such severe local tissue necrosis. Tricosanthus tricuspidata is a medicinal plant and the paste prepared from its leaves has been used extensively for the treatment of snakebite-induced tissue necrosis. Most studies including reports from our laboratory focused on plant secondary metabolite as therapeutic molecules against snakebite envenomation...
December 2, 2018: Journal of Cellular Biochemistry
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