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Marialuigia Spinelli, Lavinia Di Meglio, Beatrice Mosimann, Edoardo Di Naro, Daniel Surbek, Luigi Raio
BACKGROUND: Normal morphometry of the vermis and its relation to the posterior fossa (PF) rule out most major anomalies of the cerebellum. However, accurate categorization of the position and size of the fetal vermis remains a challenge. OBJECTIVE: Our aim was to test a new method to assess the position and size of fetal vermis on 3-dimensional ultrasound (3D-US). METHODS: We measured the vermian-crest angle (VCA) in normal fetuses using multiplanar 3D-US...
December 5, 2018: Fetal Diagnosis and Therapy
Faith Dickerson, Lorraine Jones-Brando, Glen Ford, Giulio Genovese, Cassie Stallings, Andrea Origoni, Colm O'Dushlaine, Emily Katsafanas, Kevin Sweeney, Sunil Khushalani, Robert Yolken
Background: Epstein-Barr virus (EBV) is a highly prevalent human herpesvirus capable of infecting the central nervous system and establishing persistent infection. Methods: We employed solid phase immunoassay techniques to measure immunoglobulin G (IgG) class antibodies to EBV virions and defined proteins in 432 individuals with schizophrenia and 311 individuals without a history of a psychiatric disorder. Western blot testing was performed to document reactivity to specific EBV proteins...
November 20, 2018: Schizophrenia Bulletin
Jingchen Tian, Shusen Sun, Zhigang Zhao, Xingang Li
1. This study aimed to evaluate the pharmacokinetic interaction of shuanghuanglian (SHL) and azithromycin in rats, and to provide experimental support for rational drug use in clinics. 2. HPLC-UV and HPLC-MS/MS approaches were respectively developed to detect the forsythiaside (active component of SHL) and azithromycin concentrations. Both non-compartmental and compartmental analyses were employed to calculate pharmacokinetic parameters. A nonlinear mixed-effects modelling method was applied to fit the drug concentration-time data...
November 20, 2018: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
Sohei Yamamoto, Yu Sakai
Herein, we describe an extremely rare case of gastritis due to concurrent infection with Epstein-Barr virus (EBV) and cytomegalovirus (CMV) occurring in an immunocompetent adult. The patient was a 35-year-old man who presented with slight fever, nausea, anorexia, weight loss for 3 weeks, mild transaminitis, and leukocytosis with atypical lymphocytes in peripheral blood. The clinical presentation and elevated IgM titers to both EBV-VCA and CMV strongly suggested infectious mononucleosis syndrome caused by co-infection with EBV and CMV...
November 16, 2018: Clinical Journal of Gastroenterology
Emilia Sforza, David Hupin, Frédéric Roche
Idiopathic hypersomnia (IH) is a rare central hypersomnia of unknown physiopathology. In this study, we determine if the presence of infectious mononucleosis evaluated by serological markers of Epstein Barr virus infection plays a role in this hypersomnia. Ten patients with a suspicion of IH underwent to clinical assessment, 24 h polysomnography, and serologic testing for mononucleosis including Viral Capside Antigen (VCA) IgG, the VCA IgM, and the EBV nuclear antigen (EBNA). None of the patients reported neurological inflammatory disease and viral infection prior the onset of the disease...
2018: Frontiers in Neurology
Anamaria Balić, Lucija Bartolić, Ivana Ilić, Jaka Radoš
Dear Editor,We present the case of a 40-year old male patient with lymphomatoid papulosis of a waxing and waning course on whom three biopsies were performed during a 14-year period with no change in histopathological or immunophenotypical characteristics. Lymphomatoid papulosis (LP) is a chronic, recurrent, self-healing papulonodular skin eruption with the histopathologic features of a cutaneous T-cell lymphoma but an often benign and indolent clinical course (1). It is designated as a primary, cutaneous, CD30+ lymphoproliferative disorder...
October 2018: Acta Dermatovenerologica Croatica: ADC
Yinmin Wang, Xiuxia Wang, Xianyu Zhou, Zhu Zhu, Jun Yang, Fei Liu
Adipose‑derived stem cells (ADSCs) have an immunomodulatory role in vascularized composite tissue allo‑transplantation (VCA). However, the specific effects of ADSCs on lymphocytes remain to be fully elucidated. The present study examined the changes in T cells co‑cultured with ADSCs in terms of the proliferation by Cell Counting Kit‑8 assay, cell cycle profile and apoptosis by flow cytometry, inflammatory cytokine production by polymerase chain reaction and ELISA, in addition to the expression of survival proteins by western blotting...
October 24, 2018: International Journal of Molecular Medicine
Zhao-Xia Li, Shan Zeng, Hui-Xia Wu, Yi Zhou
Previous systematic reviews have found a higher sero-prevalence of EBV antibodies in SLE patients compared with controls. Because many studies have been published, there is a need to apply more precise systematic review methods. We examined the association between EBV and SLE patients by conducting a systematic review and meta-analysis of case-control studies that examined the prevalence of EBV antibodies and the DNA-positive rate. We searched the MEDLINE and EMBASE databases from 1966 to 2018 with no language restrictions...
October 25, 2018: Clinical and Experimental Medicine
Bardia Nourbakhsh, Alice Rutatangwa, Michael Waltz, Mary Rensel, Manikum Moodley, Jennifer Graves, Theron Charles Casper, Amy Waldman, Anita Belman, Benjamin Greenberg, Manu Goyal, Yolanda Harris, Ilana Kahn, Timothy Lotze, Soe Mar, Teri Schreiner, Gregory Aaen, Janace Hart, Jayne Ness, Jennifer Rubin, Jan-Mendelt Tillema, Lauren Krupp, Mark Gorman, Leslie Benson, Moses Rodriguez, Tanuja Chitnis, John Rose, Meghan Candee, Bianca Weinstock-Guttman, Xiaorong Shao, Lisa Barcellos, Judith James, Emmanuelle Waubant
Objective: While prior Epstein-Barr virus (EBV) infection has been consistently associated with subsequent risk of developing multiple sclerosis (MS), the association with other common herpesviruses has been more controversial. Our objectives were to determine whether remote infection with EBV and other common herpesviruses affect the susceptibility to pediatric MS and if there are interactions between genetic and demographic factors and viral infections. Methods: Cases with pediatric-onset MS or clinically isolated syndrome within 4 years of disease onset, and controls were recruited from 16 American pediatric MS centers...
October 2018: Annals of Clinical and Translational Neurology
Nicholas A Smith, Paul C Baresel, Conner L Jackson, Sidney Ogolla, Eunice N Toko, Sara Heit, Erwan Piriou, Odada P Sumba, Jaap M Middeldorp, Kathryn L Colborn, Rosemary Rochford
Background: The EBV viral glycoprotein gp350 has been proposed as a candidate antigen for an EBV vaccine. However, the proposed formulations of these vaccines have not taken into account the presence of two unique EBV strains (EBV-1 and EBV-2) present in areas of high incidence of the EBV associated cancer, Burkitt's lymphoma. Methods: In this study, we analyze the kinetics of EBV-1 and EBV-2 infection in an asymptomatic infant cohort from Kisumu, Kenya. We also analyzed the kinetics of the antibody response against five EBV antigens, gp350 (IgG and IgA), VCA (IgG), EBNA-1 (IgG), EAd (IgG), and Zta (IgG)...
October 12, 2018: Journal of Infectious Diseases
Hui Lv, Lei Ye, Qian Liu, Song-Guo Li, Tao Li, Na-Li Huang, Yi Gao, Li-Bin Fan, Wei-Dong Du
PURPOSE: Epstein-Barr virus (EBV) is a ubiquitous human gamma herpes virus that infects human epithelial cells and B lymphocytes. It would be potentially valuable to develop novel combined assays to benefit screening for large panels of samples of EBV infectious diseases. EXPERIMENTAL DESIGN: A simple antigen-probed biochip that is modified with S-S-PEG-COOH and is used as a label-free high-throughput screening method for a combined detection of EBV capsid antigen IgM antibody, capsid antigen IgG antibody, and nuclear antigen IgG antibody...
October 11, 2018: Proteomics. Clinical Applications
Zeyang Wang, Zhi Lv, Hanxi Ding, Qian Xu, Liping Sun, Jingjing Jing, Yuan Yuan
The study aimed to investigate the role of serum EBV-VCA IgG in assessing gastric cancer (GC) risk and prognosis. A total of 1790 Northern Chinese participants with pathologically confirmed disease underwent EBV-VCA IgG serologic testing using enzyme-linked immunosorbent assay (ELISA), including 821 controls, 410 atrophic gastritis (AG) patients, and 559 GC patients. We found that positive EBV-VCA IgG was significantly associated with GC and its precursor, conferring a 1.55- and 1.36-fold increased risk of GC and AG, respectively (P = 0...
November 2018: Cancer Medicine
Arthur L Caplan, Brendan Parent, Jeffrey Kahn, Wendy Dean, Laura L Kimberly, W P Andrew Lee, Eduardo D Rodriguez
BACKGROUND: Despite early skepticism, the field of vascularized composite allotransplantation (VCA) has demonstrated feasibility. The ethics of VCA have moved past doubts about the morality of attempting such transplant to how to conduct them ethically. METHODS: Leaders of each program performing and/or evaluating VCA in the United States were invited to participate in a working group to assess the state and future of VCA ethics and policy. Four meetings were held over the course of 1 year to describe key challenges and potential solutions...
October 8, 2018: Transplantation
Andrew J Lindford, Heikki Mäkisalo, Hannu Jalanko, Jouni Lauronen, Veli-Jukka Anttila, Susanna Juteau, Antti-Jussi Ämmälä, Anna Eskola, Samuli Saarni, Helena Isoniemi, Antti Mäkitie, Patrik Lassus
AIM: We herein describe the establishment of the Helsinki Vascularized Composite Allotransplantation (VCA) program and its execution in the first two face transplant cases. METHODS & PATIENTS: The Helsinki VCA program initially required the fulfillment of legal, hospital, financial, and ethical requirements. Thereafter, the assembling of a multidisciplinary team commenced. A team of Plastic, maxillofacial and ENT surgeons comprise the facial VCA team. The protocol involves collaboration with the Solid Organ Transplant (SOT) team, transplant immunology, immunosuppression, microbiology, psychiatric evaluation, well-defined VCA indications and informed consent...
September 22, 2018: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
Shaun D Mendenhall, Michael T Ginnetti, Justin D Sawyer, Steven J Verhulst, Bradford L West, L Scott Levin, Michael W Neumeister
Background: Vascularized composite allotransplantation (VCA) is an emerging and growing field. Little is known about the prevalence and distribution of the adult potential donor population in the United States now that it falls under the oversight of the United Network for Organ Sharing (UNOS). Methods: We assessed the UNOS database from 2008 to 2015 to estimate the prevalence and distribution of adult potential vascularized composite allograft donors. Donor inclusion and exclusion criteria were developed in a way to minimize risk to recipients and were applied to the dataset...
June 2018: Plastic and Reconstructive Surgery. Global Open
Jessica B Chang, William J Rifkin, Marc A Soares, April Duckworth, Nakul Rao, Yee Cheng Low, Jonathan P Massie, Piul S Rabbani, Pierre B Saadeh, Daniel J Ceradini
Background: Widespread application of vascularized composite allotransplantation (VCA) is currently limited by the required lifelong systemic immunosuppression and its associated morbidity and mortality. This study evaluated the efficacy of ex vivo (after procurement but before transplantation) engineering of allografts using small interfering RNA to knockdown major histocompatibility complex I (MHC-I) and prolong rejection-free survival. Methods: Endothelial cells (ECs) were transfected with small interfering RNA targeted against MHC-I (siMHC-I) for all in vitro experiments...
June 2018: Plastic and Reconstructive Surgery. Global Open
Min Ji Sohn, Jin Min Cho, Jin Soo Moon, Jae Sung Ko, Hye Ran Yang
Primary Epstein-Barr virus (EBV) infection is common in childhood, and dual positivity of serum EBV IgM and cytomegalovirus (CMV) IgM antibodies occur in some cases. This study aimed to evaluate the cause of EBV and CMV IgM dual positivity to determine whether it represents a false-positive finding or a true coinfection.A total of 494 children diagnosed with primary EBV infection, manifesting as infectious mononucleosis, were recruited. The diagnosis was based on positive EBV viral capsid antigen (VCA) IgM antibodies, and serum CMV IgM antibodies and liver enzymes were also evaluated in 149 subjects...
September 2018: Medicine (Baltimore)
Jennifer L Wainright, Christopher L Wholley, John Rosendale, Wida S Cherikh, Darren Di Battista, David K Klassen
BACKGROUND: Vascularized composite allograft (VCA) transplants include diverse organ types and are made possible primarily by deceased donors. METHODS: We used Organ Procurement and Transplantation Network (OPTN) data to characterize VCA deceased donors (n=66 of 70) in the United States 1998-2017 and compare their characteristics with those of kidney donors in 2017. RESULTS: Through December 31, 2017, 20 transplant programs performed 72 deceased donor VCA transplants, with organs donated by 70 donors, including 30 upper limb (17 unilateral and 13 bilateral) and 11 face donors...
September 17, 2018: Transplantation
Hui-Yun Cheng, Sheri K L Tay, Chih-Jen Wen, Chih-Fan Lin, Aline Yen-Ling Wang, Ling-Yi Shih, Shiao-Chin Liu, Eiji Kobayashi, Cheng-Hung Lin, Fu-Chan Wei
BACKGROUND: Tipping the balance toward regulatory T cells (Tregs) through adoptive cell therapy has shown promise to induce transplantation tolerance. Although such strategy has been explored in many mice organ transplantation studies, less knowledge was available in rat systems. Furthermore, the behaviors of the transferred cells have not been well studied in real-time fashion. METHODS: Tregs from naïve LEW rats were purified in two steps with the autoMACS system...
2018: PloS One
Hamidreza Yazdi, Ara Nazarian, Jim S Wu, Arash Amiri, Poopak Hafezi, Margaret Babikian, Amin Mohamadi, Reza Pakdaman, Mohammad Ghorbanhoseini
Background: The valgus cut angle (VCA) of the distal femur in Total Knee Arthroplasty (TKA) is measured preoperatively on three-joint alignment radiographs. The anatomical axis of the femur can be described as the anatomical axis of the full length of the femur or as the anatomical axis of the distal half of the femur, which may result in different angles in some cases. During TKA, the anatomical axis of the femur is determined by intramedullary femoral guides, which may follow the distal half or near full anatomical axis, based on the length of the femoral guide...
July 2018: Archives of Bone and Joint Surgery
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