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https://www.readbyqxmd.com/read/28743163/alk-and-ros1-testing-on-lung-cancer-cytologic-samples-perspectives
#1
REVIEW
Pasquale Pisapia, Maria D Lozano, Elena Vigliar, Claudio Bellevicine, Francesco Pepe, Umberto Malapelle, Giancarlo Troncone
Cytologic sampling is the mainstay of diagnosing advanced lung cancer. Moreover, to select patients for personalized first-line or second-line treatment, epidermal growth factor receptor (EGFR) mutations and anaplastic lymphoma kinase (ALK) and c-ros oncogene 1 (ROS1) rearrangements are tested on cytologic preparations. Commercially available fluorescence in situ hybridization (FISH) and immunocytochemistry (ICC) assays have primarily been used for the identification of cells harboring ALK or ROS1 gene fusions on histologic rather than cytologic preparations...
July 25, 2017: Cancer
https://www.readbyqxmd.com/read/28742980/disease-relevant-modifications-of-the-methylome-and-transcriptome-by-particulate-matter-pm2-5-from-biomass-combustion
#2
Katharina Heßelbach, Gwang-Jin Kim, Stephan Flemming, Thomas Häupl, Marc Bonin, Regina Dornhof, Stefan Günther, Irmgard Merfort, Matjaz Humar
Exposure to particulate matter (PM) is recognized as a major health hazard, but molecular responses are still insufficiently described. We analyzed the epigenetic impact of ambient PM2.5 from biomass combustion on the methylome of primary human bronchial epithelial BEAS-2B cells using the Illumina HumanMethylation450 BeadChip. The transcriptome was determined by the Affymetrix HG-U133 Plus 2.0 Array. PM2.5 induced genome wide alterations of the DNA methylation pattern, including differentially methylated CpGs in the promoter region associated with CpG islands...
July 25, 2017: Epigenetics: Official Journal of the DNA Methylation Society
https://www.readbyqxmd.com/read/28742836/the-impact-of-met-igf-1-igf1r-expression-and-egfr-mutations-on-survival-of-patients-with-non-small-cell-lung-cancer
#3
Samer Al-Saad, Elin Richardsen, Thomas K Kilvaer, Tom Donnem, Sigve Andersen, Mehrdad Khanehkenari, Roy M Bremnes, Lill-Tove Busund
INTRODUCTION: To compare the efficacy of silver in situ hybridization (SISH) and immunohistochemistry (IHC) in detecting MET and IGF1R alterations and to investigate their prevalence and prognostic significance. A possible correlation between MET receptor expression, MET gene alterations and the two most frequent occurring EGFR gene mutations was also investigated. MATERIALS AND METHODS: Stage I to IIIA tumors from 326 patients with NSCLC were immunohistochemically tested for protein expression of MET and IGF-1...
2017: PloS One
https://www.readbyqxmd.com/read/28742247/ventana-immunohistochemistry-assay-for-anaplastic-lymphoma-kinase-gene-rearrangement-detection-in-patients-with-non-small-cell-lung-cancer-a-meta-analysis
#4
Wang Zhiwei, Jiang Yuan, Yao Yihui, Hou Xin, Chen Jingtao, Shi Lei, Duan Yongjian
BACKGROUND: The aim of this study was to evaluate the diagnostic value of Ventana immunohistochemistry (IHC) assay for anaplastic lymphoma kinase (ALK) gene rearrangement screening in patients with non-small cell lung cancer (NSCLC). METHODS: Open published studies that reported the diagnostic performance of Ventana IHC assay for ALK gene rearrangement detection in NSCLC patients were extracted from PubMed, Embase, Google scholar, Wanfang, and China National Knowledge Infrastructure...
July 25, 2017: Thoracic Cancer
https://www.readbyqxmd.com/read/28742165/system-analysis-identifies-distinct-and-common-functional-networks-governed-by-transcription-factor-ascl1-in-glioma-and-small-cell-lung-cancer
#5
Sainitin Donakonda, Swati Sinha, Shrinivas Nivrutti Dighe, Manchanahalli R Satyanarayana Rao
ASCL1 is a basic Helix-Loop-Helix transcription factor (TF), which is involved in various cellular processes like neuronal development and signaling pathways. Transcriptome profiling has shown that ASCL1 overexpression plays an important role in the development of glioma and Small Cell Lung Carcinoma (SCLC), but distinct and common molecular mechanisms regulated by ASCL1 in these cancers are unknown. In order to understand how it drives the cellular functional network in these two tumors, we generated a gene expression profile in a glioma cell line (U87MG) to identify ASCL1 gene targets by an si RNA silencing approach and then compared this with a publicly available dataset of similarly silenced SCLC (NCI-H1618 cells)...
July 25, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28740441/detection-of-ros1-rearrangement-in-non-small-cell-lung-cancer-current-and-future-perspectives
#6
REVIEW
Giulio Rossi, Genny Jocollé, Antonia Conti, Marcello Tiseo, Federica Zito Marino, Giovanni Donati, Renato Franco, Francesca Bono, Francesca Barbisan, Francesco Facchinetti
ROS1 rearrangement characterizes a small subset (1%-2%) of non-small cell lung cancer and is associated with slight/never smoking patients and adenocarcinoma histology. Identification of ROS1 rearrangement is mandatory to permit targeted therapy with specific inhibitors, demonstrating a significantly better survival when compared with conventional chemotherapy. Detection of ROS1 rearrangement is based on in situ (immunohistochemistry, fluorescence in situ hybridization) and extractive non-in situ assays. While fluorescence in situ hybridization still represents the gold standard in clinical trials, this technique may fail to recognize rearrangements of ROS1 with some gene fusion partner...
2017: Lung Cancer: Targets and Therapy
https://www.readbyqxmd.com/read/28740406/conventional-real-time-pcr-based-detection-of-t790m-using-tumor-tissue-or-blood-in-patients-with-egfr-tki-resistant-nsclc
#7
Ya-Lan Wu, Rui-Zhan Tong, Yan Zhang, Bin-Bin Hu, Ke Zheng, Zhen-Yu Ding, Feng Peng, You-Ling Gong, Yong-Mei Liu, You Lu
Blood biopsy has many advantages over tissue biopsy for diagnosing acquired T790M mutation in patients with non-small-cell lung cancer, such as being less risky and painful. New techniques with high sensitivity (eg, droplet digital PCR) show promising results during blood biopsy, but the positive rates of identification are still quite unclear. Whether there are other factors, except technology, affecting the results of blood biopsy is unclear. In this study, we used conventional amplification refractory mutation system to detect tumor tissue or blood for T790M mutation in patients clinically resistant to tyrosine kinase inhibitors...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28740365/spotlight-on-ceritinib-in-the-treatment-of-alk-nsclc-design-development-and-place-in-therapy
#8
REVIEW
Mariacarmela Santarpia, Maria Grazia Daffinà, Alessandro D'Aveni, Grazia Marabello, Alessia Liguori, Elisa Giovannetti, Niki Karachaliou, Maria Gonzalez Cao, Rafael Rosell, Giuseppe Altavilla
The identification of echinoderm microtubule-associated protein-like 4 (EML4) and anaplastic lymphoma kinase (ALK) fusion gene in non-small cell lung cancer (NSCLC) has radically changed the treatment of a subset of patients harboring this oncogenic driver. Crizotinib was the first ALK tyrosine kinase inhibitor to receive fast approval and is currently indicated as the first-line therapy for advanced, ALK-positive NSCLC patients. However, despite crizotinib's efficacy, patients almost invariably progress, with the central nervous system being one of the most common sites of relapse...
2017: Drug Design, Development and Therapy
https://www.readbyqxmd.com/read/28739734/nherf1-suppresses-lung-cancer-cell-migration-by-regulation-of-epithelial-mesenchymal-transition
#9
Fenglian Yang, Yanan Gu, Zehang Zhao, Jing Huang, Wen G Jiang, Shan Cheng
BACKGROUND/AIM: Na(+)/H(+) exchanger regulatory factor 1 (NHERF1) has been reported to interact with many cancer-related proteins and plays an important role in cancer progression. However, little is known about the biological functions of NHERF1 in lung cancer cells. The aim of the current study was to explore whether NHERF1 is involved in transforming growth factor (TGF)-β1-induced epithelial-mesenchymal transition (EMT) in non-small-cell lung cancer cells. MATERIALS AND METHODS: The expression of NHERF1 and EMT-associated markers including E-cadherin, N-cadherin, snail family transcriptional repressor 1 (SNAI1) and snail family transcriptional repressor 2 (SLUG) were analyzed by reverse transcription polymerase chain reaction (RT-PCR) and western blotting...
August 2017: Anticancer Research
https://www.readbyqxmd.com/read/28739724/therapeutic-role-of-mir-140-5p-for-the-treatment-of-non-small-cell-lung-cancer
#10
Valentina Flamini, Wen G Jiang, Yuxin Cui
BACKGROUND/AIM: Lung cancer is the second most common cancer in both men and women, after prostate and breast cancer, respectively. There are two main types of primary lung cancer, non-small cell lung cancer (NSCLC), which accounts for approximately 85-90% of all lung cancer cases, and small cell lung cancer (SCLC), which accounts for the other 10-15% of lung cancers. MiRNAs are small molecules that post-transcriptionally regulate many genes and contribute to many disease aetiologies, including tumours...
August 2017: Anticancer Research
https://www.readbyqxmd.com/read/28739589/association-of-calcium-calmodulin-dependent-protein-kinase-kinase1-rs7214723-polymorphism-with-lung-cancer-risk-in-a-chinese-population
#11
Da Chen, Fangming Zhong, Ye Chen
Calcium/calmodulin-dependent protein kinase kinase1 (CAMKK1) could specially recognize and activate Calcium/calmodulin-dependent protein kinase (CAMK) I and IV. Furthermore, The activation of CAMK showed positively correlation with proliferation of lung cancer (LC). In addition, A genome-wide association study has identified rs7214723 (E375G) in the CAMKK1 gene as a susceptibility locus for LC in the UK population. Therefore, we conducted a case-control study involving 320 LC patients and 320 controls to validate this conclusion in a Chinese population...
July 24, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28739440/genetic-variation-in-gstp1-lung-function-risk-of-lung-cancer-and-mortality-in-66-000-individuals
#12
Marianne S Nørskov, Morten Dahl, Anne Tybjærg-Hansen
INTRODUCTION: Glutathione-S-transferase (GST) P1 metabolises carcinogens from tobacco smoke in the lung. We tested whether genetically altered GSTP1 activity affects lung function and risk of tobacco-related cancer and mortality in the general population. METHODS: We genotyped 66,069 individuals from the Caucasian general population for two common, functional variants in the GSTP1 gene (Ile105Val and Ala114Val), and recorded lung function, lung cancer, tobacco-related cancer, and death as outcomes...
July 21, 2017: Journal of Thoracic Oncology
https://www.readbyqxmd.com/read/28739316/validation-of-the-disease-specific-gpa-for-patients-with-1-to-3-synchronous-brain-metastases-in-newly-diagnosed-nsclc
#13
Neil M Woody, Matthew D Greer, Chandana A Reddy, Gregory M M Videtic, Samuel T Chao, Erin S Murphy, John H Suh, Liliana Angelov, Gene H Barnett, Michael A Vogelbaum, Kevin L Stephans
BACKGROUND: The disease-specific graded prognostic assessment (DS-GPA) for brain metastases is a powerful prognostic tool but has not been validated for patients with synchronous brain metastases (SBM) in newly diagnosed non-small-cell lung cancer (NSCLC). PATIENTS AND METHODS: We identified patients with newly diagnosed NSCLC with 1 to 3 SBM treated with stereotactic radiosurgery (SRS) between 1997 and 2012. We included patients whose brain metastases were treated with SRS alone or combined SRS and whole-brain radiotherapy (WBRT)...
July 6, 2017: Clinical Lung Cancer
https://www.readbyqxmd.com/read/28738961/-circular-rna-circhipk3-promotes-nci-h1299-and-nci-h2170-cell-proliferation-through-mir-379-and-its-target-igf1
#14
Fang Tian, Yun Wang, Zhe Xiao, Xuejun Zhu
BACKGROUND: It has been proven that the circular RNA, possessing a stable covalently closed continuous loop, is a type of RNA molecule which is expressed widespread in mammals. The circular RNA circHIPK3 is abundantly expressed in hepatocellular carcinoma (HCC) and promotes tumourgenesis. However, a role for circHIPK3 has not been systematically examined in non-small cell lung cancer (NSCLC). In this study, we investigated whether circHIPK3 has an effect on cell proliferation in the NSCLC cell lines NCI-H1299 and NCI-H2170 and the underlying molecular mechanisms...
July 20, 2017: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
https://www.readbyqxmd.com/read/28738960/-mir-218-inhibits-migration-and-invasion-of-lung-cancer-cell-%C3%A2-by-regulating-robo1-expression
#15
Ping Chen, Yunlong Zhao, Yingjie Li
BACKGROUND: To explore the function and the potential molecular mechanism of miR-218 in lung cancer cell. METHODS: The expression of miR-218 mRNA was determined by real-time PCR in lung cancer tissues, adjacent tissues and lung cancer cells. Transwell assay was used to detect the migration and invasion of A549 cell after transfected with Anti-miR-218 or negative control and HC4006 cell after transfected with miR-218 mimics and miR-218 negative control. Targetscan and MiRanda were used to calculate the potential targets of miR-218 and Luciferase reporter assay was performed to identify that the Robo1 was one target genes of miR-218...
July 20, 2017: Zhongguo Fei Ai za Zhi, Chinese Journal of Lung Cancer
https://www.readbyqxmd.com/read/28738500/mir-101-3p-inhibits-the-growth-and-metastasis-of-non-small-cell-lung-cancer-through-blocking-pi3k-akt-signal-pathway-by-targeting-malat-1
#16
Xiaoqiang Zhang, Xianju He, Yunbing Liu, Huiqing Zhang, He Chen, Shanxian Guo, Yonggang Liang
BACKGROUND: MiR-101-3p is an important tumor suppressor miRNA in many human cancers. This study was to investigate the role of miR-101-3p in the progression of non-small cell lung cancer (NSCLC) and its potential underlying mechanism. METHODS: In this study, the endogenous expression of miR-101-3p or MALAT-1 in cells was modulated by cell transfection assays. The regulatory interaction of miR-101-3p and MALAT-1 was examined by Luciferase reporter gene and RNA pull-down assays...
July 18, 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28734892/correlation-between-rna-seq-and-microarrays-results-using-tcga-data
#17
Li Chen, Fenghao Sun, Xiaodong Yang, Yulin Jin, Mengkun Shi, Lin Wang, Yu Shi, Cheng Zhan, Qun Wang
RNA sequencing (RNA-Seq) and microarray are two of the most commonly used high-throughput technologies for transcriptome profiling; however, they both have their own inherent strengths and limitations. This research aims to analyze the correlation between microarrays and RNA-Seq detection of transcripts in the same tissue sample to explore the reproducibility between the techniques. Using data of RNA-Seq v2 and three different microarrays provided by The Cancer Genome Atlas, 11,120 genes of 111 lung squamous cell carcinoma samples were simultaneously detected by the four methods...
July 19, 2017: Gene
https://www.readbyqxmd.com/read/28734822/icotinib-versus-whole-brain-irradiation-in-patients-with-egfr-mutant-non-small-cell-lung-cancer-and-multiple-brain-metastases-brain-a-multicentre-phase-3-open-label-parallel-randomised-controlled-trial
#18
Jin-Ji Yang, Caicun Zhou, Yisheng Huang, Jifeng Feng, Sun Lu, Yong Song, Cheng Huang, Gang Wu, Li Zhang, Ying Cheng, Chengping Hu, Gongyan Chen, Li Zhang, Xiaoqing Liu, Hong Hong Yan, Fen Lai Tan, Wenzhao Zhong, Yi-Long Wu
BACKGROUND: For patients with non-small-cell lung cancer (NSCLC) and multiple brain metastases, whole-brain irradiation (WBI) is a standard-of-care treatment, but its effects on neurocognition are complex and concerning. We compared the efficacy of an epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitor (TKI), icotinib, versus WBI with or without chemotherapy in a phase 3 trial of patients with EGFR-mutant NSCLC and multiple brain metastases. METHODS: We did a multicentre, open-label, parallel randomised controlled trial (BRAIN) at 17 hospitals in China...
July 19, 2017: Lancet Respiratory Medicine
https://www.readbyqxmd.com/read/28734041/mir-103-functions-as-a-tumor-suppressor-by-directly-targeting-programmed-cell-death-10-in-nsclc
#19
Dong Yang, Jian-Jun Wang, Jin-Song Li, Qian-Yu Xu
NSCLC accounts for about 85% of all lung cancer cases. Absence of miR-103 has recently identified to be associated withmetastatic capacity of primary lung tumors. However, the exact role of miR-103 in NSCLC and the molecular mechanism is unclear. In the present study, we showed that miR-103 expression was reduced in NSCLC tissues and cells. miR-103 expression was negatively correlated with tumor size and stage. The overall survival was longer in patients with higher miR-103 level than in those with lower miR-103 expression...
July 21, 2017: Oncology Research
https://www.readbyqxmd.com/read/28732213/comparison-of-blood-collection-tubes-from-three-different-manufacturers-for-the-collection-of-cell-free-dna-for-liquid-biopsy-mutation-testing
#20
Christina Alidousty, Danielle Brandes, Carina Heydt, Svenja Wagener, Maike Wittersheim, Stephan Christian Schäfer, Barbara Holz, Sabine Merkelbach-Bruse, Reinhard Büttner, Jana Fassunke, Anne Maria Schultheis
The improvement in sensitive techniques has allowed the detection of tumor-specific aberrations in circulating tumor (ct) DNA. Amplification-refractory mutation system PCR has been used for the analysis of ctDNA to detect resistance-causing mutations in the epidermal growth factor receptor gene (eg, EGFR T790M) in lung cancer patients. So far, Streck tubes have been widely used as standard blood collection tubes. Here, we compared blood collection tubes manufactured by Streck with tubes from Roche and Qiagen regarding their utility in stabilizing ctDNA in plasma samples...
July 18, 2017: Journal of Molecular Diagnostics: JMD
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