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Recovery from stem cell treatment

Toshimitsu Otani, Daigo Ochiai, Hirotaka Masuda, Yushi Abe, Marie Fukutake, Tadashi Matsumoto, Kei Miyakoshi, Mamoru Tanaka
BACKGROUND: Hypoxic-ischemic encephalopathy (HIE) remains a major cause of cerebral palsy. Increasing evidence has suggested that mesenchymal stem cells have a favorable effect on HIE. However, the efficacy of human amniotic fluid stem cells (hAFS) for HIE, especially in the chronic phase, remains unclear. The aim of this study was to determine the neurorestorative effect of hAFS on the chronic phase of HIE. METHODS: hAFS were isolated from AF cells as CD117-positive cells...
August 10, 2018: Pediatric Research
Lijian Shao, Wuping Yang, Rui Xu, Shuqin Zhu, Yanqiu Huang, Huan Li, Xincheng Wu, Mengzhen Yue, Xiaoliang Xiong, Xiaowen Chen, Bohai Kuang, Guangqin Fan, Qingxian Zhu, Huihong Zeng
BACKGROUND: Irradiation-induced kidney damage is inevitable during radiotherapeutic practice, which limits effective radiotherapy doses on tumor treatment. In the present study, the role of mTOR complex 1 (mTORC1) signaling was investigated in irradiation-induced renal injuries. METHODS: Mice were exposed to 8.0-Gy X-ray of total body irradiation and subsequently treated with rapamycin. Changes of renal morphology were assessed by hematoxylin and eosin staining...
August 14, 2018: Stem Cell Research & Therapy
Laura Otero-Ortega, Fernando Laso-García, MariCarmen Gómez-de Frutos, Blanca Fuentes, Luke Diekhorst, Exuperio Díez-Tejedor, María Gutiérrez-Fernández
Approximately, 16 million strokes occur worldwide each year, causing 6 million deaths and considerable disability, implying an enormous social, individual health, and economic burden. Due to this high incidence, strategies to promote stroke recovery are urgently needed. Research into new therapeutic approaches for stroke has determined that intravenous administration of mesenchymal stem cells (MSCs) is a good strategy to improve recovery by amplifying mechanisms implicated in brain plasticity. Recent studies have demonstrated the efficacy of MSCs in stroke, with no need for them to reach the area of brain injury...
August 13, 2018: Translational Stroke Research
Chong Wang, Haibing Yue, Qian Feng, Bingzhe Xu, Liming Bian, Peng Shi
Traumatic injury in the central nervous system can lead to loss of functional neurons. Transplantation of neural progenitors is a promising therapeutic strategy. However, infusion of dissociated cells often suffers from low viability, uneven cell distribution, and poor in vivo engraftment that could be reinforced by a better cell delivery system. Here, we develop an injectable composite-hydrogel-system for use as a minimally invasive treatment of spinal cord injury (SCI) using motor neurons derived from embryonic stem cells (ESCs)...
August 9, 2018: ACS Applied Materials & Interfaces
Xi Chen, Hao Wu, Hansen Chen, Qi Wang, Xue-Jiao Xie, Jiangang Shen
Radix Astragali (AR) is a commonly used medicinal herb for post-stroke disability in Traditional Chinese Medicine but its active compounds for promoting neurogenic effects are largely unknown. In the present study, we tested the hypothesis that Astragaloside VI could be a promising active compound from AR for adult neurogenesis and brain repair via targeting epidermal growth factor (EGF)-mediated MAPK signaling pathway in post-stroke treatment. By using cultured neural stem cells (NSCs) and experimental stroke rat model, we investigated the effects of Astragaloside VI on inducing NSCs proliferation and self-renewal in vitro, and enhancing neurogenesis for the recovery of the neurological functions in post-ischemic brains in vivo...
August 7, 2018: Molecular Neurobiology
Tanongsak Laowanitwattana, Sirinda Aungsuchawan, Suteera Narakornsak, Runchana Markmee, Waleephan Tancharoen, Junjira Keawdee, Nonglak Boonma, Witoon Tasuya, Lamaiporn Peerapapong, Nathaporn Pangjaidee, Peeraphan Pothacharoen
Osteoporosis is a bone degenerative disease characterized by a decrease in bone strength and an alteration in the osseous micro-architecture causing an increase in the risk of fractures. These diseases usually happen in post-menopausal women and elderly men. The most common treatment involves anti-resorptive agent drugs. However, the inhibition of bone resorption alone is not adequate for recovery in patients at the severe stage of osteoporosis who already have a fracture. Therefore, the combination of utilizing osteoblast micro mimetic scaffold in cultivation with the stimulation of osteoblastic differentiations to regain bone formation is a treatment strategy of considerable interest...
August 2, 2018: Acta Histochemica
Liana Monteiro da Fonseca Cardoso, Lucio Filgueiras Pacheco Moreira, Marcelo Alves Pinto, Andrea Henriques-Pons, Luiz Anastácio Alves
Background and Aims: Acute liver failure (ALF) is a severe syndrome with an elevated mortality rate, ranging from 40 to 80 %. Currently, liver transplantation is the only definitive treatment for these patients and new therapies aiming to treat ALF include artificial organs implant and stem cells therapy, for example. However, a major limitation of liver donors exists. Living donor liver transplantation (LDLT), split liver transplantation (SLT), and domino liver transplantation (DLT) are some of the available alternatives to treat ALF patients, but these do not reduce the number of patients on waiting lists...
2018: Canadian Journal of Gastroenterology & Hepatology
Gerhard Bauer, Magdi Elsallab, Mohamed Abou-El-Enein
The promise of stem cell (SC) therapies to restore functions of damaged tissues and organs brings enormous hope to patients, their families, loved ones and caregivers. However, limits may exist for which indications SC therapies might be useful, efficacious, and safe. Applications of innovative therapies within regulatory boundaries and within the framework of controlled clinical trials are the norm in the scientific and medical community; such a system minimizes patient risk by setting a clear and acceptable safety and efficacy profile for new therapeutics before marketing authorization...
July 31, 2018: Stem Cells Translational Medicine
Daisuke Mori, Shigeru Miyagawa, Shin Yajima, Shunsuke Saito, Satsuki Fukushima, Takayoshi Ueno, Koichi Toda, Kotoe Kawai, Hayato Kurata, Hiroyuki Nishida, Kayako Isohashi, Jun Hatazawa, Yoshiki Sawa
BACKGROUND: Allogeneic adipose-derived mesenchymal stem cells (ADSC) are promising cell sources for cell therapy to treat ischemic cardiomyopathy (ICM).We hypothesized that ADSC transplantation via the new cell spray method may be a feasible, safe, and effective treatment for ICM. METHODS: Human ADSCs were acquired from white adipose tissue. Porcine ICM models were established by constriction of the left anterior descending coronary artery. ADSCs were spread over the surface of the heart via cell spray in fibrinogen and thrombin solutions...
July 25, 2018: Transplantation
Kee-Hang Lee, Hee-Jang Pyeon, Hyun Nam, Jeong-Seob Won, Ji-Yoon Hwang, Kyung-A Lee, Je Young Yeon, Seung-Chyul Hong, Do-Hyun Nam, Kyunghoon Lee, Sun-Ho Lee, Kyeung-Min Joo
Neural stem cells are emerging as a regenerative therapy for spinal cord injury (SCI), since they differentiate into functional neural cells and secrete beneficial paracrine factors into the damaged microenvironment. Previously, we successfully isolated and cultured adult human multipotent neural cells (ahMNCs) from the temporal lobes of epileptic patients. In this study, we investigated the therapeutic efficacy and treatment mechanism of ahMNCs for SCI using rodent models. When 1 × 106 ahMNCs were transplanted into injured spinal cords at 7 days after contusion, the injection group showed significantly better functional recovery than the control group (media injection after contusion), which was determined by the Basso, Beattie and Bresnahan (BBB) score...
July 19, 2018: Stem Cell Research
Guido Lancman, Alexander Coltoff, Amir Steinberg
Thrombocytopenia is a relatively common complication following allogeneic hematopoietic stem cell transplantation and is associated with increased bleeding, transfusion requirements, chronic graft-versus-host disease, and all-cause mortality. There are currently no approved treatments outside of supportive transfusions. We report on the outcomes of five patients at our institution who received romiplostim for either primary engraftment failure or secondary failure of platelet recovery following stem cell transplantation...
July 17, 2018: Hematology/oncology and Stem Cell Therapy
Francesco De Francesco, Silvia Mannucci, Giamaica Conti, Elena Dai Prè, Andrea Sbarbati, Michele Riccio
Adipose tissue possesses phenotypic gene expression characteristics that are similar to human mesenchymal stem cells (hMSCs). Nevertheless, the multilineage potential may be inhibited, and cells may not expand adequately to satisfy the requirements of Good Manufacturing Practice (cGMP). An autologous hMSC-enriched fat product would fulfil the void from a biomedical and clinical perspective. In this study, we suggest a novel mechanism using a closed system without enzymes, additives or other modifications, which will produce non-expanded, accessible material...
July 15, 2018: International Journal of Molecular Sciences
Xue-Mei Zhang, Jiao Ma, Yang Sun, Bing-Qian Yu, Zhuo-Min Jiao, Duo Wang, Mei-Yu Yu, Jin-Yue Li, Jin Fu
BACKGROUND: Spinal cord injury (SCI) is one of the most severe central nervous system injuries. Currently, transplanting bone marrow mesenchymal stem cells (BMSCs) is considered a therapeutic option for SCI. Tanshinone IIA (TIIA) is one of the extracts obtained from Salvia miltiorrhiza Bunge, which has been shown to have some protective effects against SCI. The present research was aimed to explore whether TIIA would influence the fate of transplanted BMSCs in a rat model of SCI, especially with regard to their differentiation into neuronal cells...
July 13, 2018: Journal of Translational Medicine
Niketa A Patel, Lauren Daly Moss, Jea-Young Lee, Naoki Tajiri, Sandra Acosta, Charles Hudson, Sajan Parag, Denise R Cooper, Cesario V Borlongan, Paula C Bickford
BACKGROUND: Neuroinflammation is a common therapeutic target for traumatic brain injury (TBI) due to its contribution to delayed secondary cell death and has the potential to occur for years after the initial insult. Exosomes from adipose-derived stem cells (hASCs) containing the long noncoding RNA MALAT1 are a novel, cell-free regenerative approach to long-term recovery after traumatic brain injury (TBI) that have the potential to modulate inflammation at the genomic level. The long noncoding RNA MALAT1 has been shown to be an important component of the secretome of hASCs...
July 12, 2018: Journal of Neuroinflammation
Maurizio D Baroni, Sonia Colombo, Enzo Martegani
Aspirin and its main metabolite salicylate are promising molecules in preventing cancer and metabolic diseases. S. cerevisiae cells have been used to study some of their effects: (i) salicylate induces the reversible inhibition of both glucose transport and the biosyntheses of glucose-derived sugar phosphates, (ii) Aspirin/salicylate causes apoptosis associated with superoxide radical accumulation or early cell necrosis in MnSOD-deficient cells growing in ethanol or in glucose, respectively. So, treatment with (acetyl)-salicylic acid can alter the yeast metabolism and is associated with cell death...
March 26, 2018: Microbial Cell
Daisuke Tanokashira, Eiko Kurata, Wataru Fukuokaya, Kenshiro Kawabe, Mana Kashiwada, Hideyuki Takeuchi, Masamitsu Nakazato, Akiko Taguchi
Age-related reduction in adult hippocampal neurogenesis is correlated with cognitive impairment. Diabetes is a chronic systemic disease that negatively affects adult neural stem cells and memory functions in the hippocampus. Despite growing concern regarding the potential role of diabetic drugs in neural abnormalities, their effects on progressive deterioration of neurogenesis and cognitive functions remain unknown. Here, we show that the combination of aging and diabetes in mice causes a marked decrease in hippocampal neurogenesis along with memory impairment and elevated neuroinflammation...
July 2018: FEBS Open Bio
Qian Liu, Yaxi Zhu, Peter C Amadio, Steven L Moran, Anne Gingery, Chunfeng Zhao
Tendon injuries are among the most common and severe hand injuries with a high demand for functional recovery. Stem cells have been identified and isolated from different species and a variety of tissues for the sake of regenerative medicine. Recently, turkey has been suggested as a potential new large animal model for flexor tendon-related research. However, turkey tissue-specific stem cells have not been investigated. Here, we presented the isolation and verification of tendon-derived stem cells (TDSCs) from 6- to 8-month-old heritage-breed turkey...
2018: Stem Cells International
Hiroo Kimura, Takehito Ouchi, Shinsuke Shibata, Tsuyoshi Amemiya, Narihito Nagoshi, Taneaki Nakagawa, Morio Matsumoto, Hideyuki Okano, Masaya Nakamura, Kazuki Sato
Strategies for therapeutic cell transplantation have been assessed for use in the treatment of massive peripheral nerve defects. To support safe and efficient cell transplantation, we have focused on the purification of cells using cell surface markers. Our group previously reported low-affinity nerve growth factor receptor (LNGFR)- and thymocyte antigen-1 (THY-1)-positive neural crest-like cells (LT-NCLCs), generated from human induced pluripotent stem cells (hiPSCs). In the present study, we investigated the efficacy of transplantation of hiPSC-derived LT-NCLCs in a murine massive peripheral nerve defect model...
July 3, 2018: Scientific Reports
Bing Zheng, Shang-Fan Yu, Geoffrey Del Rosario, Steven R Leong, Genee Y Lee, Rajesh Vij, Cecilia P C Chiu, Wei-Ching Liang, Yan Wu, Cecile Chalouni, Jack Sadowsky, Vanessa M Clark, Angela Hendricks, Kirsten Achilles Poon, Yu-Waye Chu, Thomas H Pillow, M M Schutten, John A Flygare, Andrew G Polson
PURPOSE: The treatment of acute myeloid leukemia (AML) has not significantly changed in 40 years. Cytarabine and anthracycline‑based chemotherapy induction regimens (7 + 3) remain the standard of care, and most patients have poor long-term survival. The re-approval of Mylotarg, an anti-CD33-calicheamicin antibody-drug conjugate (ADC), has demonstrated ADCs as a clinically validated option to enhance the effectiveness of induction therapy. We are interested in developing a next generation ADC for AML to improve upon the initial success of Mylotarg...
June 29, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Anna Bosch-Vilaseca, Irene García-Cadenas, Elisa Roldán, Silvana Novelli, Pere Barba, Albert Esquirol, David Valcárcel, Rodrigo Martino, Jorge Sierra
OBJECTIVE AND METHODS: Severe postengraftment thrombocytopenia is a common complication after allogeneic stem cell transplantation (alloSCT). A few studies have suggested that the use of thrombopoietin agonists (TPOa) may be useful in this setting. Our retrospective study is the largest series published to date; we retrospectively evaluated TPOa efficacy and safety in 20 adult alloSCT recipients who received TPOa as a compassionate use for clinically relevant thrombocytopenia. RESULTS: Twelve of 20 patients (60%) responded, with a 180-day cumulative incidence of successful platelet recovery to ≥30 and ≥50 × 109 /L of 57% (95% CI: 44%-71%) and 32% (95% CI: 18%-46%), respectively, which were reached at a median of 28 and 34 days from the start of therapy...
June 29, 2018: European Journal of Haematology
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