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Recovery from stem cell treatment

Nicholas White, Shelly Sakiyama-Elbert
Due to the nature of the biological response to traumatic spinal cord injury, there are very limited therapeutic options available to patients. Recent advances in cell transplantation have demonstrated the therapeutic potential of transplanting supportive cell types following spinal cord injury. In particular, pluripotent stem cell derived neural cells are of interest for the future investigation. Use of pluripotent stem cells as the source allows many cell types to be produced from a population that can be expanded in vitro...
October 15, 2018: Developmental Dynamics: An Official Publication of the American Association of Anatomists
Tatsuya Osaki, Sebastien G M Uzel, Roger D Kamm
Amyotrophic lateral sclerosis (ALS), a progressive neurodegenerative disease involving loss of motor neurons (MNs) and muscle atrophy, still has no effective treatment, despite much research effort. To provide a platform for testing drug candidates and investigating the pathogenesis of ALS, we developed an ALS-on-a-chip technology (i.e., an ALS motor unit) using three-dimensional skeletal muscle bundles along with induced pluripotent stem cell (iPSC)-derived and light-sensitive channelrhodopsin-2-induced MN spheroids from a patient with sporadic ALS...
October 2018: Science Advances
Gabriel Tremblay, Mike Dolph, Sachin Patel, Patricia Brandt, Anna Forsythe
Aims: Midostaurin (MIDO) has been proposed for the treatment of newly-diagnosed adult patients with FMS-like tyrosine kinase 3 mutation-positive (FLT3+) acute myeloid leukemia (AML) in combination with standard chemotherapy. The cost-effectiveness of MIDO and standard of care (SOC) followed by MIDO monotherapy was compared to SOC alone for newly-diagnosed FLT3+ AML in the UK. Methods: A partitioned survival model was developed from a UK public healthcare system perspective to compare the cost-effectiveness of MIDO plus SOC and SOC over a lifetime horizon...
2018: Cost Effectiveness and Resource Allocation: C/E
Sima T Bhatt, Jeffrey J Bednarski, Julia Berg, Kathryn Trinkaus, Lisa Murray, Robert Hayashi, Ginny Schulz, Monica Hente, Michael Grimley, Ka Wah Chan, Naynesh Kamani, David Jacobsohn, Michael Nieder, Gregory Hale, Lolie Yu, Roberta Adams, Jignesh Dalal, Michael A Pulsipher, Paul Haut, Sonali Chaudhury, Jeffrey Davis, Jennifer Jaroscak, Martin Andreansky, Jennifer Willert, Shalini Shenoy
Hematopoietic stem cell transplantation (HSCT) is a therapeutic option for many nonmalignant disorders (NMD) and is curative or prevents disease progression. Reduced intensity conditioning (RIC) in HSCT for NMD may reduce regimen-related acute toxicities and late complications. Myeloablation is often replaced by immune suppression in RIC regimens to support donor engraftment. The pace of immune reconstitution following immune suppression by RIC regimens is influenced by agents used, donor source, and graft-versus-host disease (GVHD) prophylaxis/treatment...
October 12, 2018: Biology of Blood and Marrow Transplantation
Hojjat-Allah Abbaszadeh, Somayeh Niknazar, Shahram Darabi, Navid Ahmady Roozbahany, Ali Noori-Zadeh, Seyed Kamran Ghoreishi, Maryam Sadat Khoramgah, Yousef Sadeghi
Spinal cord injury is a significant cause of motor dysfunctions. There is no definite cure for it, and most of the therapeutic modalities are only symptomatic treatment. In this systematic review and meta-analysis, the effectiveness of stem cell therapy in the treatment of the spinal cord injuries in animal models was studied and evaluated. A systematic search through medical databases by using appropriate keywords was conducted. The relevant reports were reviewed in order to find out cases in which inclusion and exclusion criteria had been fulfilled...
September 2018: Anatomy & Cell Biology
Lingling Yang, Songmei Zhang, Haoyun Duan, Muchen Dong, Xiaoli Hu, Zhaohua Zhang, Yao Wang, Xiaoping Zhang, Weiyun Shi, Qingjun Zhou
Chronic inflammation and severe dry eye are two important adverse factors for the successful transplant of cultured limbal stem cells. The aim of this study was to investigate the effects of inflammation and hyperosmotic stress (a key pathological factor in dry eye) on corneal epithelial stem cells (CESCs) and corneal epithelial wound healing. We observed that the CESCs exhibited significant morphological changes when treated with either interleukin-1 beta (IL-1β), tumor necrosis factor alpha (TNF-α), or hyperosmotic stress...
October 9, 2018: Stem Cells Translational Medicine
Yaqiong Tang, Xinyou Zhang, Shiyu Han, Tiantian Chu, Jiaqian Qi, Hong Wang, Xiaowen Tang, Huiying Qiu, Chengcheng Fu, Changgeng Ruan, Depei Wu, Yue Han
Severe thrombocytopenia is a serious condition that frequently arises in patients with myelodysplastic syndrome (MDS) and is associated with poor prognosis. Few studies have investigated the prognostic significance of platelet recovery in patients with MDS having thrombocytopenia. We retrospectively analyzed 117 patients with de novo MDS complicated with thrombocytopenia (platelet count [PLT] < 100 × 109 /L). Patients received decitabine treatment (schedule A) or decitabine followed by allogeneic hematopoietic stem cell transplantation (allo-HSCT; schedule B)...
October 8, 2018: Clinical and Applied Thrombosis/hemostasis
Anna S Świerzko, Mateusz Michalski, Anna Sokołowska, Mateusz Nowicki, Łukasz Eppa, Agnieszka Szala-Poździej, Iwona Mitrus, Anna Szmigielska-Kapłon, Małgorzata Sobczyk-Kruszelnicka, Katarzyna Michalak, Aleksandra Gołos, Agnieszka Wierzbowska, Sebastian Giebel, Krzysztof Jamroziak, Marek L Kowalski, Olga Brzezińska, Steffen Thiel, Jens C Jensenius, Katarzyna Kasperkiewicz, Maciej Cedzyński
We conducted a prospective study of 312 patients (194 with multiple myeloma, 118 with lymphomas) receiving high-dose conditioning chemotherapy and autologous hematopoietic stem cell transplantation (auto-HSCT). Polymorphisms of MBL2 and MASP2 genes were investigated and serial measurements of serum concentrations of mannose-binding lectin (MBL), CL-LK collectin and MASP-2 as well as activities of MBL-MASP-1 and MBL-MASP-2 complex were made. Serum samples were taken before conditioning chemotherapy, before HSCT and once weekly after (totally 4-5 samples); in minority of subjects also 1 and/or 3 months post transplantation...
2018: Frontiers in Immunology
Bohao Liu, Benjamin W Lee, Koki Nakanishi, Aranzazu Villasante, Rebecca Williamson, Jordan Metz, Jinho Kim, Mariko Kanai, Lynn Bi, Kristy Brown, Gilbert Di Paolo, Shunichi Homma, Peter A Sims, Veli K Topkara, Gordana Vunjak-Novakovic
The ability of extracellular vesicles (EVs) to regulate a broad range of cellular processes has recently been exploited for the treatment of diseases. For example, EVs secreted by stem cells injected into infarcted hearts can induce recovery through the delivery of stem-cell-specific miRNAs. However, the retention of the EVs and the therapeutic effects are short-lived. Here, we show that an engineered hydrogel patch capable of slowly releasing EVs secreted from cardiomyocytes derived from induced pluripotent stem (iPS) cells reduced arrhythmic burden, promoted ejection-fraction recovery, decreased cardiomyocyte apoptosis 24 hours after infarction, and reduced infarct size and cell hypertrophy 4 weeks post-infarction when implanted onto infarcted rat hearts...
May 2018: Nature Biomedical Engineering
Bo Hou, Meiqin Cai, Chuan Chen, Wanqing Ji, Zhuopeng Ye, Cong Ling, Zhuopeng Chen, Ying Guo
Autologous nerves, artificial scaffolds or acellular nerve scaffolds are commonly used in bridging treatment for peripheral nerve defects. Xenogeneic acellular nerve scaffolds and allogeneic cellular nerve scaffolds have the same structural characteristics. Due to the wider source of raw materials, these latter scaffolds have high-potential value for applications. However, whether their heterogeneity will affect nerve regeneration is unknown. The current study evaluated the efficiency of xenogeneic acellular nerve scaffolds (XANs) combined with 5-ethynyl-2'-deoxyuridine (EdU)-labeling of autologous bone marrow-derived stem cells (BMSCs) for repair of a 1...
September 7, 2018: Journal of Biomedical Materials Research. Part A
Marta S Carvalho, Atharva A Poundarik, Joaquim M S Cabral, Cláudia L da Silva, Deepak Vashishth
Bone regeneration, following fracture, relies on autologous and allogenic bone grafts. However, majority of fracture population consists of older individuals with poor quality bone associated with loss and/or modification of matrix proteins critical for bone formation and mineralization. Allografts suffer from same limitations and carry the risk of delayed healing, infection, immune rejection and eventual fracture. In this work, we apply a synergistic biomimetic strategy to develop matrices that rapidly form bone tissue - a critical aspect of fracture healing of weight bearing bones...
September 26, 2018: Scientific Reports
Eduardo Farias Sanches, Lauren Valentim, Felipe de Almeida Sassi, Lisiane Bernardi, Nice Arteni, Simone Nardin Weis, Felipe Kawa Odorcyk, Patricia Pranke, Carlos Alexandre Netto
Neonatal hypoxia-ischemia (HI) is associated to cognitive and motor impairments and until the moment there is no proven treatment. The underlying neuroprotective mechanisms of stem cells are partially understood and include decrease in excitotoxicity, apoptosis and inflammation suppression. This study was conducted in order to test the effects of intracardiac transplantation of human dental pulp stem cells (hDPSCs) for treating HI damage. Seven-day-old Wistar rats were divided into four groups: sham-saline, sham-hDPSCs, HI-saline, and HI-hDPSCs...
September 25, 2018: Neurochemical Research
Eytan M Stein, Min Yang, Annie Guerin, Wei Gao, Philip Galebach, Cheryl Q Xiang, Subrata Bhattacharyya, Gaetano Bonifacio, George J Joseph
BACKGROUND: Preference valuations of health status are essential in health technology and economic appraisal. This study estimated utilities for treatment-related health states of acute myeloid leukemia (AML) and disutilities of severe adverse events (SAEs) using a representative sample of adults from the general population in the United States (US). METHODS: Treatment-related AML health states, defined based on literature and interviews with clinicians, included complete remission (CR), no CR, relapse, stem cell transplant (SCT), and post SCT short-term recovery...
September 21, 2018: Health and Quality of Life Outcomes
Lifeng Qi, Xinhong Xue, Jijun Sun, Qingjian Wu, Hongru Wang, Yan Guo, Baoliang Sun
Many studies have reported the recovery ability of umbilical cord-derived mesenchymal stem cells (UC-MSCs) for neural diseases. In this study, the authors explored the roles of UC-MSCs to treat the traumatic brain injury. Umbilical cord-derived mesenchymal stem cells were isolated from healthy neonatal rat umbilical cord immediately after delivery. The traumatic brain injury (TBI) model was formed by the classical gravity method. The authors detected the behavior changes and measured the levels of inflammatory factors, such as interleukin-lβ and tumor necrosis factor-α by enzyme linked immunosorbent assay (ELISA) at 1, 2, 3, 4 weeks after transplantation between TBI treated and untreated with UC-MSCs...
October 2018: Journal of Craniofacial Surgery
Fernando Laso-García, Jaime Ramos-Cejudo, Francisco Javier Carrillo-Salinas, Laura Otero-Ortega, Ana Feliú, MariCarmen Gómez-de Frutos, Miriam Mecha, Exuperio Díez-Tejedor, Carmen Guaza, María Gutiérrez-Fernández
Extracellular vesicles (EVs) have emerged as important mediators of intercellular communication and as possible therapeutic agents in inflammation-mediated demyelinating diseases, including multiple sclerosis (MS). In the present study, we investigated whether intravenously administered EVs derived from mesenchymal stem cells (MSCs) from human adipose tissue might mediate recovery in Theiler's murine encephalomyelitis virus (TMEV)-induced demyelinating disease, a progressive model of MS. SJL/J mice were subjected to EV treatment once the disease was established...
2018: PloS One
Ahmed Al Bayati, Thomas Plate, Mahmood Al Bayati, Yaohong Yan, Efrat Saraf Lavi, Joseph D Rosenblatt
Erdheim-Chester disease (ECD) is a rare form of non-Langerhans cell histiocytosis characterized by infiltration of organs by CD68+ and CD1a- lipid-laden histiocytes, including the central nervous system in more than a third of patients. Molecular analysis of ECD samples has demonstrated the prevalence of BRAF V600E mutations as high as 54%. Recently, vemurafenib became the only Food and Drug Administration-approved treatment for patients with ECD who carry the BRAF V600E mutation. However, dabrafenib has been suggested to have greater brain distribution...
September 2018: Mayo Clinic proceedings. Innovations, quality & outcomes
Liming Qing, Huanwen Chen, Juyu Tang, Xiaofeng Jia
Peripheral nerve injury is a major clinical problem and often results in a poor functional recovery. Despite obvious clinical need, treatment strategies have been largely suboptimal. In the nervous system, exosomes, which are nanosized extracellular vesicles, play a critical role in mediating intercellular communication. More specifically, microRNA carried by exosomes are involved in various key processes such as nerve and vascular regeneration, and exosomes originating from Schwann cells, macrophages, and mesenchymal stem cells can promote peripheral nerve regeneration...
September 2018: Neurorehabilitation and Neural Repair
Jianbang Han, Zhiming Feng, Yu Xie, Feng Li, Bingke Lv, Tian Hua, Zhongfei Zhang, Chengmei Sun, Dazhuang Su, Qian Ouyang, Yingqian Cai, Yuxi Zou, Yanping Tang, Haitao Sun, Xiaodan Jiang
Bone marrow-derived mesenchymal stem cells (BMSCs) exhibit potential regenerative effects on the injured brain. However, these effects are constrained by their limited ability to migrate to the injured site. Oncostatin M (OSM) has been shown to affect the proliferation and migration of mesenchymal stem cells. Therefore, in the present study, we explored whether OSM improves BMSC migration and secretion of growth factors and cytokines in a rat middle cerebral artery occlusion (MCAO) stroke model. The effect of OSM on the proliferation and apoptosis of rat BMSCs was first assessed in vitro, and the gene and secretion levels of factors related to cell nutrition and migration, such as SDF-1 and VEGF, were detected...
September 10, 2018: Experimental Neurology
Chaitra Venugopal, K Shobha, Kiranmai S Rai, Venkata Bharatkumar Pinnelli, Bindu M Kutty, Anandh Dhanushkodi
Mesenchymal stem cell (MSC) therapy in recent years have gained significant attention. Though the functional outcomes following MSC therapy for neurodegenerative diseases are convincing, various mechanisms for the functional recovery are being debated. Nevertheless, recent studies convincingly demonstrated that recovery following MSC therapy could be reiterated with MSC secretome per se thereby shifting the dogma from cell therapy to cell "based" therapy. In addition to various functional proteins, stem cell secretome also includes extracellular membrane vesicles like exosomes...
September 13, 2018: Current Gene Therapy
Zaal Kokaia, Vladimer Darsalia
Ischemic stroke is the second most common cause of death worldwide and a major cause of disability. It takes place when the brain does not receive sufficient blood supply due to the blood clot in the vessels or narrowing of vessels' inner space due to accumulation of fat products. Apart from thrombolysis (dissolving of blood clot) and thrombectomy (surgical removal of blood clot or widening of vessel inner area) during the first hours after an ischemic stroke, no effective treatment to improve functional recovery exists in the post-ischemic phase...
2018: Results and Problems in Cell Differentiation
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