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barcoding Diptera

Chufei Tang, Ding Yang, Patrick Grootaert
In the present paper the Oriental species of the genus Lichtwardtia Enderlein, 1912 are revised based on the type material of known species and new material from Singapore and Cambodia. A re-description and illustration of the holotype female of Lichtwardtiaziczac (Wiedemann, 1824) is given but since it has been described on the basis of a female only and its provenance India Orientalis is only a vague indication of its type locality, it is considered as a nomen dubium. All the species put as junior synonyms by Becker (1922) of L...
2018: ZooKeys
Mame Thierno Bakhoum, Mamadou Sarr, Assane Gueye Fall, Karine Huber, Moussa Fall, Mbacké Sembène, Momar Talla Seck, Karien Labuschagne, Laetitia Gardès, Mamadou Ciss, Geoffrey Gimonneau, Jérémy Bouyer, Thierry Baldet, Claire Garros
BACKGROUND: Biting midge species of the genus Culicoides Latreille (Diptera: Ceratopogonidae) comprise more than 1300 species distributed worldwide. Several species of Culicoides are vectors of various viruses that can affect animals, like the African horse sickness virus (AHSV), known to be endemic in sub-Saharan Africa. The ecological and veterinary interest of Culicoides emphasizes the need for rapid and reliable identification of vector species. However, morphology-based identification has limitations and warrants integration of molecular data...
December 3, 2018: Parasites & Vectors
Hiroyuki Takaoka, VAN Lun Low, Tiong Kai Tan, Mohd Sofian-Azirun, Chee Dhang Chen, Koon Weng Lau, Xuan DA Pham
Simulium pumatense sp. nov. is described from Vietnam, and is placed in the Simulium feuerborni species-group of the subgenus Simulium (Nevermannia) Enderlein. Its morphological characteristics include the relatively smaller numbers of the following three numerical features: inner teeth of the female mandible (15-18), minute conical processes (16) on the female cibarium, and male upper-eye facets (in 15 vertical columns and 16 horizontal rows). Keys are constructed to distinguish this species from four species of the same group in Vietnam...
November 26, 2018: Acta Tropica
John Smit, Ivo Raemakers, Kevin Beentjes
Thick-headed flies of the genus Myopa Fabricius are a common sight in early spring in Europe. Several species can be found flying among Salix catkins together with their supposed Andrena Fabricius hosts (Hymenoptera: Apidae) (John Smit observations). Despite the fact that some of these Myopa species are very common, little is known about their actual host-parasitoid associations. Only a few scattered host records can be found in the literature, most of which refer to M. testacea s.l.. Whether or not these records actually concern M...
November 15, 2018: Zootaxa
Mikko Tiusanen, Tea Huotari, Paul D N Hebert, Tommi Andersson, Ashley Asmus, Joël Bêty, Emma Davis, Jennifer Gale, Bess Hardwick, David Hik, Christian Körner, Richard B Lanctot, Maarten J J E Loonen, Rauni Partanen, Karissa Reischke, Sarah T Saalfeld, Fanny Senez-Gagnon, Paul A Smith, Ján Šulavík, Ilkka Syvänperä, Christine Urbanowicz, Sian Williams, Paul Woodard, Yulia Zaika, Tomas Roslin
Pollination is an ecosystem function of global importance. Yet, who visits the flower of specific plants, how the composition of these visitors varies in space and time, and how such variation translates into pollination services is hard to establish. The use of DNA barcodes allows us to address ecological patterns involving thousands of taxa that are difficult to identify. To clarify the regional variation in the visitor community of a wide-spread flower resource, we compared the composition of the arthropod community visiting species in the genus Dryas (mountain avens, family Rosaceae), throughout Arctic and high-alpine areas...
November 12, 2018: Molecular Ecology
Gunnar Mikalsen Kvifte, Marija IvkoviĆ
Pericoma miljenkoi sp. nov. is described based on adult males from the Plitvička jezera National Park, Croatia. Pericoma trifasciata is recorded from Croatia for the first time, and COI DNA barcodes are given from specimens collected in Germany. An emended key to adult Pericoma of the trifasciata group is presented. The Croatian Psychodidae fauna now stands at 35 species.
September 26, 2018: Zootaxa
Jelena PreradoviĆ, Andrijana AndriĆ, SneŽana RadenkoviĆ, Ljiljana ŠaŠiĆ ZoriĆ, Celeste PÉrez-baÑÓn, AndrÉs Campoy, Ante VujiĆ
plants, are mostly unknown. All known immature stages of Merodon feed on underground storage organs (bulbs, rhizomes and corms) of geophytes of the families Asparagaceae, Iridaceae and Amaryllidaceae. Of 160 known Merodon species, to date, the pupal stages have been described for only four: M. equestris (Fabricius), M. bombiformis Hull, M. luteihumerus Marcos-García, Vujić Mengual, and M. geniculatus Strobl. During field investigations in Đerdap National Park, Serbia, Merodon puparia were found in the ground near the bulbs of Ornithogalum umbellatum L...
May 16, 2018: Zootaxa
Atsuya Kodama, Koichiro Kawai, Hidetoshi Saito
A new species of the genus Tanytarsus van der Wulp, 1874 was described and illustrated based on an adult male. Tanytarsus trichovalis sp. nov. belongs to the eminulus species group, and is closely related to T. tamaundecimus Sasa, 1980, T. okuboi Sasa, 1986, and T. tonebeceus Sasa Tanaka, 2000. However, the species is distinguished from these species by the morphology of the hypopygium. We also present the first record of T. ovatus Johannsen, 1932 in Japan. In addition, DNA barcoding of 5 species including T...
May 25, 2018: Zootaxa
Wojciech GiŁka, Lauri Paasivirta, Piotr Gadawski, MichaŁ Grabowski
Tanytarsus latens sp. nov. is described from Finland (Ostrobothnia borealis, Satakunta). Both morphological and             molecular analyses indicate that T. latens belongs to the mendax species group. The adult male hypopygium of the new species resembles that of Tanytarsus occultus Brundin and of T. desertor Giłka et Paasivirta, while the molecular analysis based on the mitochondrial cytochrome oxidase (COI) gene fragment evidences that T. latens is a sister species to most of European Tanytarsus of the mendax group's core, for which the COI barcodes are known...
September 6, 2018: Zootaxa
Tenielle Cooke, Kyle Sieghard Kulenkampff, Marise Heyns, Laura Jane Heathfield
Forensic entomology aids the determination of post mortem interval based on arthropods associated with a deceased body. This relies on the accurate identification of insects that visit the body, particularly first colonisers such as Calliphoridae (Diptera). Traditional species identification though morphological keys can be challenging as immature or closely related specimens can look similar. Some of these challenges have been overcome through 'DNA barcoding', which involves the sequencing of informative regions within a species' DNA and comparison to a database of reference sequences...
October 2, 2018: Genome Génome / Conseil National de Recherches Canada
Takehiro K Katoh, Guang Zhang, Masanori J Toda, Awit Suwito, Jian-Jun Gao
The subgenus Dudaica Strand of the genus Drosophila Fallén has been known to comprise only two species: Drosophila (Dudaica) senilis Duda, 1926 (recorded from Indonesia, Philippines, Vietnam, Bhutan, and India) and D.malayana (Takada, 1976) (recorded from Malaysia). In the present study, this subgenus is revised, with D.malayana redescribed and six new species discovered and described from China, Malaysia, and Indonesia: gracilipalpis Katoh & Gao, sp. n. , puberula Katoh & Gao, sp. n. , albipalpis Katoh, Toda & Gao, sp...
2018: ZooKeys
Bilgenur Baloğlu, Esther Clews, Rudolf Meier
Background: Macroinvertebrates such as non-biting midges (Chironomidae: Diptera) are important components of freshwater ecosystems. However, they are often neglected in biodiversity and conservation research because invertebrate species richness is difficult and expensive to quantify with traditional methods. We here demonstrate that Next Generation Sequencing barcodes ("NGS barcodes") can provide relief because they allow for fast and large-scale species-level sorting of large samples at low cost...
2018: Frontiers in Zoology
Ignacio Ruiz-Arrondo, Luis M Hernández-Triana, Aleksandra Ignjatović-Ćupina, Nadya Nikolova, Javier Alfonso Garza-Hernández, Mario Alberto Rodríguez-Pérez, José A Oteo, Anthony R Fooks, Javier Lucientes Curdi
BACKGROUND: Blackflies have negative impact on public and animal health due to the haematophagous habit of females. In recent times, in some regions in Spain, blackfly outbreaks are becoming more and more frequent, threatening the public health. However, there is still a paucity of data concerning the Spanish blackfly fauna. Correct identification of species is of paramount importance in order to provide correct information on species distribution, biology and behaviour, so that control measures could be implemented appropriately...
August 13, 2018: Parasites & Vectors
Tanasak Changbunjong, Benjaporn Bhusri, Poonyapat Sedwisai, Thekhawet Weluwanarak, Eakanan Nitiyamatawat, Theeraphap Chareonviriyaphap, Jiraporn Ruangsittichai
Horse flies (Diptera: Tabanidae) are of medical and veterinary importance because they are known to transmit pathogens. Approximately 80 species of horse flies have been reported in Thailand. Monitoring the distribution of horse fly species is important to control the spread of diseases transmitted by them. Currently, the species identification of horse flies is based on their morphology; this requires considerable skills and taxonomic expertise, and it may be difficult to identify morphologically similar species...
August 15, 2018: Veterinary Parasitology
Wei Chen, Yanjie Shang, Lipin Ren, Kai Xie, Xiangyan Zhang, Changquan Zhang, Shule Sun, Yong Wang, Lagabaiyila Zha, Yadong Guo
Some representatives of flesh flies visiting/colonizing the decomposed remains demonstrated their values in estimating the minimal postmortem interval (PMImin) since death. However, the utility of sarcophagid flies has been seriously hampered by limited ecological, biological and taxonomic knowledge of them. Although mitochondrial genes have been proposed as a potential DNA barcode for the species-level identification of sarcophagids, some defects still remain such as the substantial memory and processing time taken for homologous comparisons online...
September 2018: Forensic Science International
Van Lun Low, Hiroyuki Takaoka, Peter H Adler, Tiong Kai Tan, Francis Cheng-Hsuan Weng, Cheng-Yu Chen, Yvonne Ai Lian Lim, Zubaidah Ya'cob, Chee Dhang Chen, Mohd Sofian-Azirun, Daryi Wang
The Simulium rufibasis subgroup is one of three subgroups of the Simulium (Simulium) tuberosum species-group; it is characterized by a pair of clustered stout hairs on the ventral surface of female abdominal segment 7. A member of the S. rufibasis subgroup in Taiwan was investigated morphologically and genetically using the universal cytochrome c oxidase subunit I (COI) barcoding gene and polytene chromosomal banding pattern. The Taiwanese material is morphologically similar to S. rosliramlii Takaoka & Chen from Vietnam and represents the second species of the S...
July 14, 2018: Parasitology Research
Muhammad Ashfaq, Jamal S M Sabir, Hosam O El-Ansary, Kate Perez, Valerie Levesque-Beaudin, Arif M Khan, Akhtar Rasool, Carlene Gallant, Joseph Addesi, Paul D N Hebert
Although insects dominate the terrestrial fauna, sampling constraints and the poor taxonomic knowledge of many groups have limited assessments of their diversity. Passive sampling techniques and DNA-based species assignments now make it possible to overcome these barriers. For example, Malaise traps collect specimens with minimal intervention while the Barcode Index Number (BIN) system automates taxonomic assignments. The present study employs Malaise traps and DNA barcoding to extend understanding of insect diversity in one of the least known zoogeographic regions, the Saharo-Arabian...
2018: PloS One
Matt N Krosch, Francesca Strutt, Mark J Blacket, Jana Batovska, Melissa Starkie, Anthony R Clarke, Stephen L Cameron, Mark K Schutze
Accurate species-level identifications underpin many aspects of basic and applied biology; however, identifications can be hampered by a lack of discriminating morphological characters, taxonomic expertise or time. Molecular approaches, such as DNA "barcoding" of the cytochrome c oxidase (COI) gene, are argued to overcome these issues. However, nuclear encoding of mitochondrial genes (numts) and poor amplification success of suboptimally preserved specimens can lead to erroneous identifications. One insect group for which these molecular and morphological problems are significant are the dacine fruit flies (Diptera: Tephritidae: Dacini)...
June 6, 2018: Insect Science
Oumama Benabdelkrim Filali, Mostafa Kabine, Adil El Hamouchi, Meryem Lemrani, Mustapha Debboun, M'hammed Sarih
Anopheles sergentii known as the "oasis vector" or the "desert malaria vector" is considered the main vector of malaria in the southern parts of Morocco. Its presence in Morocco is confirmed for the first time through sequencing of mitochondrial DNA (mDNA) cytochrome c oxidase subunit I (COI) barcodes and nuclear ribosomal DNA (rDNA) second internal transcribed spacer (ITS2) sequences and direct comparison with specimens of A. sergentii of other countries. The DNA barcodes (n = 39) obtained from A...
September 2018: Vector Borne and Zoonotic Diseases
Edward Pfeiler
Genetic diversity in mitochondrial DNA barcodes, comprising a segment of the cytochrome c oxidase subunit I (COI) gene, was used to infer demographic histories in selected taxa of the cactophilic Drosophila repleta species group in North America. Haplotype and nucleotide diversities were determined in 16 taxa based on both previously published and new sequences. Haplotype diversity (h) differed dramatically in different taxa, varying from h = 0 in D. eremophila, D. hexastigma and D. bifurca to h = 0.99 in D...
May 21, 2018: Journal of Heredity
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