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barcoding Diptera

Gunnar Mikalsen Kvifte, Marija IvkoviĆ
Pericoma miljenkoi sp. nov. is described based on adult males from the Plitvička jezera National Park, Croatia. Pericoma trifasciata is recorded from Croatia for the first time, and COI DNA barcodes are given from specimens collected in Germany. An emended key to adult Pericoma of the trifasciata group is presented. The Croatian Psychodidae fauna now stands at 35 species.
September 26, 2018: Zootaxa
Jelena PreradoviĆ, Andrijana AndriĆ, SneŽana RadenkoviĆ, Ljiljana ŠaŠiĆ ZoriĆ, Celeste PÉrez-baÑÓn, AndrÉs Campoy, Ante VujiĆ
plants, are mostly unknown. All known immature stages of Merodon feed on underground storage organs (bulbs, rhizomes and corms) of geophytes of the families Asparagaceae, Iridaceae and Amaryllidaceae. Of 160 known Merodon species, to date, the pupal stages have been described for only four: M. equestris (Fabricius), M. bombiformis Hull, M. luteihumerus Marcos-García, Vujić Mengual, and M. geniculatus Strobl. During field investigations in Đerdap National Park, Serbia, Merodon puparia were found in the ground near the bulbs of Ornithogalum umbellatum L...
May 16, 2018: Zootaxa
Atsuya Kodama, Koichiro Kawai, Hidetoshi Saito
A new species of the genus Tanytarsus van der Wulp, 1874 was described and illustrated based on an adult male. Tanytarsus trichovalis sp. nov. belongs to the eminulus species group, and is closely related to T. tamaundecimus Sasa, 1980, T. okuboi Sasa, 1986, and T. tonebeceus Sasa Tanaka, 2000. However, the species is distinguished from these species by the morphology of the hypopygium. We also present the first record of T. ovatus Johannsen, 1932 in Japan. In addition, DNA barcoding of 5 species including T...
May 25, 2018: Zootaxa
Wojciech GiŁka, Lauri Paasivirta, Piotr Gadawski, MichaŁ Grabowski
Tanytarsus latens sp. nov. is described from Finland (Ostrobothnia borealis, Satakunta). Both morphological and             molecular analyses indicate that T. latens belongs to the mendax species group. The adult male hypopygium of the new species resembles that of Tanytarsus occultus Brundin and of T. desertor Giłka et Paasivirta, while the molecular analysis based on the mitochondrial cytochrome oxidase (COI) gene fragment evidences that T. latens is a sister species to most of European Tanytarsus of the mendax group's core, for which the COI barcodes are known...
September 6, 2018: Zootaxa
Tenielle Cooke, Kyle Sieghard Kulenkampff, Marise Heyns, Laura Jane Heathfield
Forensic entomology aids the determination of post mortem interval based on arthropods associated with a deceased body. This relies on the accurate identification of insects that visit the body, particularly first colonisers such as Calliphoridae (Diptera). Traditional species identification though morphological keys can be challenging as immature or closely related specimens can look similar. Some of these challenges have been overcome through 'DNA barcoding', which involves the sequencing of informative regions within a species' DNA and comparison to a database of reference sequences...
October 2, 2018: Genome Génome / Conseil National de Recherches Canada
Takehiro K Katoh, Guang Zhang, Masanori J Toda, Awit Suwito, Jian-Jun Gao
The subgenus Dudaica Strand of the genus Drosophila Fallén has been known to comprise only two species: Drosophila (Dudaica) senilis Duda, 1926 (recorded from Indonesia, Philippines, Vietnam, Bhutan, and India) and D.malayana (Takada, 1976) (recorded from Malaysia). In the present study, this subgenus is revised, with D.malayana redescribed and six new species discovered and described from China, Malaysia, and Indonesia: gracilipalpis Katoh & Gao, sp. n. , puberula Katoh & Gao, sp. n. , albipalpis Katoh, Toda & Gao, sp...
2018: ZooKeys
Bilgenur Baloğlu, Esther Clews, Rudolf Meier
Background: Macroinvertebrates such as non-biting midges (Chironomidae: Diptera) are important components of freshwater ecosystems. However, they are often neglected in biodiversity and conservation research because invertebrate species richness is difficult and expensive to quantify with traditional methods. We here demonstrate that Next Generation Sequencing barcodes ("NGS barcodes") can provide relief because they allow for fast and large-scale species-level sorting of large samples at low cost...
2018: Frontiers in Zoology
Ignacio Ruiz-Arrondo, Luis M Hernández-Triana, Aleksandra Ignjatović-Ćupina, Nadya Nikolova, Javier Alfonso Garza-Hernández, Mario Alberto Rodríguez-Pérez, José A Oteo, Anthony R Fooks, Javier Lucientes Curdi
BACKGROUND: Blackflies have negative impact on public and animal health due to the haematophagous habit of females. In recent times, in some regions in Spain, blackfly outbreaks are becoming more and more frequent, threatening the public health. However, there is still a paucity of data concerning the Spanish blackfly fauna. Correct identification of species is of paramount importance in order to provide correct information on species distribution, biology and behaviour, so that control measures could be implemented appropriately...
August 13, 2018: Parasites & Vectors
Tanasak Changbunjong, Benjaporn Bhusri, Poonyapat Sedwisai, Thekhawet Weluwanarak, Eakanan Nitiyamatawat, Theeraphap Chareonviriyaphap, Jiraporn Ruangsittichai
Horse flies (Diptera: Tabanidae) are of medical and veterinary importance because they are known to transmit pathogens. Approximately 80 species of horse flies have been reported in Thailand. Monitoring the distribution of horse fly species is important to control the spread of diseases transmitted by them. Currently, the species identification of horse flies is based on their morphology; this requires considerable skills and taxonomic expertise, and it may be difficult to identify morphologically similar species...
August 15, 2018: Veterinary Parasitology
Wei Chen, Yanjie Shang, Lipin Ren, Kai Xie, Xiangyan Zhang, Changquan Zhang, Shule Sun, Yong Wang, Lagabaiyila Zha, Yadong Guo
Some representatives of flesh flies visiting/colonizing the decomposed remains demonstrated their values in estimating the minimal postmortem interval (PMImin) since death. However, the utility of sarcophagid flies has been seriously hampered by limited ecological, biological and taxonomic knowledge of them. Although mitochondrial genes have been proposed as a potential DNA barcode for the species-level identification of sarcophagids, some defects still remain such as the substantial memory and processing time taken for homologous comparisons online...
September 2018: Forensic Science International
Van Lun Low, Hiroyuki Takaoka, Peter H Adler, Tiong Kai Tan, Francis Cheng-Hsuan Weng, Cheng-Yu Chen, Yvonne Ai Lian Lim, Zubaidah Ya'cob, Chee Dhang Chen, Mohd Sofian-Azirun, Daryi Wang
The Simulium rufibasis subgroup is one of three subgroups of the Simulium (Simulium) tuberosum species-group; it is characterized by a pair of clustered stout hairs on the ventral surface of female abdominal segment 7. A member of the S. rufibasis subgroup in Taiwan was investigated morphologically and genetically using the universal cytochrome c oxidase subunit I (COI) barcoding gene and polytene chromosomal banding pattern. The Taiwanese material is morphologically similar to S. rosliramlii Takaoka & Chen from Vietnam and represents the second species of the S...
July 14, 2018: Parasitology Research
Muhammad Ashfaq, Jamal S M Sabir, Hosam O El-Ansary, Kate Perez, Valerie Levesque-Beaudin, Arif M Khan, Akhtar Rasool, Carlene Gallant, Joseph Addesi, Paul D N Hebert
Although insects dominate the terrestrial fauna, sampling constraints and the poor taxonomic knowledge of many groups have limited assessments of their diversity. Passive sampling techniques and DNA-based species assignments now make it possible to overcome these barriers. For example, Malaise traps collect specimens with minimal intervention while the Barcode Index Number (BIN) system automates taxonomic assignments. The present study employs Malaise traps and DNA barcoding to extend understanding of insect diversity in one of the least known zoogeographic regions, the Saharo-Arabian...
2018: PloS One
Matt N Krosch, Francesca Strutt, Mark J Blacket, Jana Batovska, Melissa Starkie, Anthony R Clarke, Stephen L Cameron, Mark K Schutze
Accurate species-level identifications underpin many aspects of basic and applied biology; however, identifications can be hampered by a lack of discriminating morphological characters, taxonomic expertise or time. Molecular approaches, such as DNA "barcoding" of the cytochrome c oxidase (COI) gene, are argued to overcome these issues. However, nuclear encoding of mitochondrial genes (numts) and poor amplification success of suboptimally preserved specimens can lead to erroneous identifications. One insect group for which these molecular and morphological problems are significant are the dacine fruit flies (Diptera: Tephritidae: Dacini)...
June 6, 2018: Insect Science
Oumama Benabdelkrim Filali, Mostafa Kabine, Adil El Hamouchi, Meryem Lemrani, Mustapha Debboun, M'hammed Sarih
Anopheles sergentii known as the "oasis vector" or the "desert malaria vector" is considered the main vector of malaria in the southern parts of Morocco. Its presence in Morocco is confirmed for the first time through sequencing of mitochondrial DNA (mDNA) cytochrome c oxidase subunit I (COI) barcodes and nuclear ribosomal DNA (rDNA) second internal transcribed spacer (ITS2) sequences and direct comparison with specimens of A. sergentii of other countries. The DNA barcodes (n = 39) obtained from A...
September 2018: Vector Borne and Zoonotic Diseases
Edward Pfeiler
Genetic diversity in mitochondrial DNA barcodes, comprising a segment of the cytochrome c oxidase subunit I (COI) gene, was used to infer demographic histories in selected taxa of the cactophilic Drosophila repleta species group in North America. Haplotype and nucleotide diversities were determined in 16 taxa based on both previously published and new sequences. Haplotype diversity (h) differed dramatically in different taxa, varying from h = 0 in D. eremophila, D. hexastigma and D. bifurca to h = 0.99 in D...
May 21, 2018: Journal of Heredity
Lipin Ren, Wei Chen, Yanjie Shang, Fanming Meng, Lagabaiyila Zha, Yong Wang, Yadong Guo
Muscid Flies (Diptera: Muscidae) are of great forensic importance due to their wide distribution, ubiquitous and synanthropic nature. They are frequently neglected as they tend to arrive at the corpses later than the flesh flies and blow flies. Moreover, the lack of species-level identification also hinders investigation of medicolegal purposes. To overcome the difficulty of morphological identification, molecular method has gained relevance. Cytochrome c oxidase subunit I (COI) gene has been widely utilized...
August 29, 2018: Journal of Medical Entomology
Salman Shayya, Régis Debruyne, André Nel, Dany Azar
Calliphoridae are among the first insects associated to decomposing animal remains. We have collected 1,841 specimens of three calliphorid genera: Calliphora, Lucilia, and Chrysomya, from different Lebanese localities as a first step in implementing a database of insects of forensic relevance for the country. Blow-flies are crucial for the estimation of the postmortem interval. DNA-based identification is a rapid and accurate method, often used for morphologically similar species, especially for immatures or incomplete specimens...
August 29, 2018: Journal of Medical Entomology
Burçin Morçiçek, Belgin Gocmen Taskin, Ersin Doğaç, Taylan Doğaroğlu, Vatan Taskin
Establishing reliable risk projection information about the distribution pattern of members of the Culex pipiens complex is of particular interest, as these mosquitoes are competent vectors for certain disease-causing pathogens. Wolbachia, a maternally inherited bacterial symbiont, are distributed in various arthropod species and can induce cytoplasmic incompatibility, i.e., reduced egg hatch, in certain crosses. It is being considered as a tool for population control of mosquito disease vectors. The Aegean region is characterized by highly populated, rural, and agricultural areas and is also on the route of the migratory birds...
June 2018: Journal of Vector Ecology: Journal of the Society for Vector Ecology
Sarah Susanne Übleis, Claudia Cuk, Michaela Nawratil, Julia Butter, Ellen Schoener, Adelheid G Obwaller, Thomas Zechmeister, Georg G Duscher, Franz Rubel, Karin Lebl, Carina Zittra, Hans-Peter Fuehrer
Information on mosquito-borne filarioid helminths in Austria is scarce, but recent discoveries of Dirofilaria repens indicate autochthonous distribution of this parasite in Eastern Austria. In the current xenomonitoring study, more than 48,000 mosquitoes were collected in Eastern Austria between 2013 and 2015, using different sampling techniques and storage conditions, and were analysed in pools with molecular tools for the presence of filarioid helminth DNA. Overall, DNA of D. repens , Setaria tundra , and two unknown filarioid helminths were documented in twenty mosquito pools within the mitochondrial cox1 gene (barcode region)...
2018: Canadian Journal of Infectious Diseases & Medical Microbiology
D Li, D W Waite, D N Gunawardana, B McCarthy, D Anderson, A Flynn, S George
Immature fruit fly stages of the family Tephritidae are commonly intercepted on breadfruit from Pacific countries at the New Zealand border but are unable to be identified to the species level using morphological characters. Subsequent molecular identification showed that they belong to Bactrocera xanthodes, which is part of a species complex that includes Bactrocera paraxanthodes, Bactrocera neoxanthodes and an undescribed species. To establish a more reliable molecular identification system for B. xanthodes, a reference database of DNA barcode sequences for the 5'-fragment of COI gene region was constructed for B...
May 6, 2018: Bulletin of Entomological Research
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