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Epithelial to mesenchymal transition lung

Bornita Das, Dona Sinha
Globally, non-small cell lung cancer is a leading cause of cancer-related mortality and about 40% of these cancers are detected in the metastatic stage. Epithelial mesenchymal transition (EMT) plays a critical role during malignant transformation, and the extracellular matrix component, fibronectin (FN), is a known inducer of invasion and metastasis. Diallyl disulphide (DADS), a bioactive component of garlic, exhibits a wide spectrum of biological activities including the inhibition of cancer cell migration and invasion...
December 5, 2018: Food & Function
Biao Geng, Manman Liang, Lilong Qin, Wenying Zhao, Hanli Wang, Lijing Wang, Xianhui Pan, Xingwu Chen
Lung cancer (LC) is a devastating malignancy with no effective treatments, due to its complex genomic profile. Using bioinformatics analysis and immunohistochemical of lung carcinoma tissues, we show that TRIM59 as a critical oncoprotein relating to LC proliferation and metastasis. In this study, high TRIM59 expression was significantly correlated with lymph node metastasis, distant metastasis, and tumour stage. Furthermore, up-regulation of TRIM59 expression correlated with poorer outcomes in LC patients. Mechanistically, TRIM59 play a key role in promoting LC growth and metastasis through regulation of extracellular-signal regulated protein kinase (ERK) signalling pathway and epithelial-to-mesenchymal transition (EMT)-markers, as validated by loss-of-function studies...
December 4, 2018: Journal of Cellular and Molecular Medicine
Deli Hong, Andrew J Fritz, Jonathan A Gordon, Coralee E Tye, Joseph R Boyd, Kirsten M Tracy, Seth E Frietze, Frances E Carr, Jeffrey A Nickerson, Andre J Van Wijnen, Anthony N Imbalzano, Sayyed K Zaidi, Jane B Lian, Janet L Stein, Gary S Stein
The RUNX1 transcription factor has recently been shown to be obligatory for normal development. RUNX1 controls the expression of genes essential for proper development in many cell lineages and tissues including blood, bone, cartilage, hair follicles, and mammary glands. Compromised RUNX1 regulation is associated with many cancers. In this review, we highlight evidence for RUNX1 control in both invertebrate and mammalian development and recent novel findings of perturbed RUNX1 control in breast cancer that has implications for other solid tumors...
December 4, 2018: Journal of Cellular Physiology
Xiao-Ming Hou, Shu-Qiao Yuan, Da Zhao, Xiao-Jun Liu, Xin-An Wu
Lactate dehydrogenase A (LDH-A) is a key enzyme during glycolysis, which increases the synthesis of related proteins and has elevated activity in cancer cells. The role of LDH-A in lung adenocarcinoma (LUAD) progression was investigated in this study. Expression levels of LDH-A were assessed in LUAD samples,and the relationship between LDH-A expression status and the prognosis of LUAD patients was confirmed. The effect of LDH-A on proliferation, invasion, migration, and colony formation of cancer cells was assessed...
December 3, 2018: Bioscience Reports
Cheng-Che Lee, Yun-Ching Cheng, Chi-Yen Chang, Chi-Min Lin, Jang-Yang Chang
MEC-17, a newly identified alpha-tubulin-N-acetyltransferase 1, serves as the major α-tubulin acetyltransferase to promote α-tubulin acetylation in vitro and in vivo. Alteration of α-tubulin acetylation may be involved in morphology regulation, cell migration, and tumour metastasis. However, MEC-17's role in cell physiology and its effect on epithelial-mesenchymal transition (EMT) and cell polarity remain elusive. In the present study, we characterized the overexpressed or downregulated cell models through gene targeting as MEC-17 gain- or loss-of-function...
November 30, 2018: Scientific Reports
U-Ging Lo, Rey-Chen Pong, Diane Yang, Leah Gandee, Elizabeth Hernandez, Andrew Dang, Chun-Jung Lin, John Santoyo, Shihong Ma, Rajni Sonavane, Jun Huang, Shu-Fen Tseng, Loredana Moro, Arnaldo A Arbini, Payal Kapur, Ganesh V Raj, Dalin He, Chih-Ho Lai, Ho Lin, Jer-Tsong Hsieh
Interferon-γ (IFNγ), a potent cytokine known to modulate tumor immunity and tumoricidal effects, is highly elevated in prostate cancer (PCa) patients after radiation. In this study, we demonstrate that IFNγ can induce epithelial-to-mesenchymal transition (EMT) in PCa cells via the JAK-STAT signaling pathway, leading to the transcription of IFN-stimulated genes (ISG) such as interferon-induced tetratricopeptide repeat 5 (IFIT5). We unveil a new function of IFIT5 complex in degrading precursor microRNAs (pre-miRNA) that include pre-miR-363 from the miR-106a-363 cluster as well as pre-miR-101 and pre-miR-128, who share a similar 5'-end structure with pre-miR-363...
November 30, 2018: Cancer Research
Nien-Tsu Hsieh, Chun-Yin Huang, Chien-Cheng Li, I-Ching Wang, Ming-Fen Lee
Non-small-cell lung cancer (NSCLC) accounts for the majority of the lung cancer cases that have become a leading cause of cancer deaths worldwide. Overexpression of transcription factor forkhead box M1 (FOXM1) is involved in the inauspicious development of several types of cancer, including lung tumor aggressiveness. Our laboratory has previously found that MED28, a Mediator subunit for transcriptional activation, modulates cell growth, epithelial-mesenchymal transition, migration, and invasion in human breast cancer cells...
November 29, 2018: Journal of Cellular Physiology
Xiaoqing Zheng, Yifan Chen, Man Bai, Yuxi Liu, Binyuan Xu, Ruoxuan Sun, Huihui Zeng
Treating cancer metastasis is of vital importance to prolong patients' survival. Thioredoxin reductase (TrxR) is overexpressed in many cancer types and has been recognized as an anti-cancer target. The organoselenium compound ethaselen (BBSKE) has been proved to be a TrxR inhibitor and inhibit various types of tumor growth. However, whether BBSKE could inhibit tumor metastasis remains unclear. In this study, we aim to explore the antimetastatic effect of BBSKE and underlying mechanisms. BBSKE was found to dose-dependently suppress migration and invasion of MCF-7 and LoVo cells in vitro...
November 26, 2018: Free Radical Biology & Medicine
Yixuan Wang, Junjie Piao, Qianrong Wang, Xuelian Cui, Ziqi Meng, Tiefeng Jin, Zhenhua Lin
The expression and biological function of Paip1 remains poorly understood in most human cancers. The objective of this research is to investigate its clinical significance and roles in lung adenocarcinoma (LADC). Immunohistochemistry was used to determine Paip1 expression in 58 cases of LADC patients with strict follow-up and 60 cases of adjacent normal lung tissues. Paip1 protein was upregulated in 77.6% (45/58) LADC tissues compared with adjacent normal lung tissues. The overexpression of Paip1 was significantly correlated with histological grade, clinical stage and poor prognosis...
November 26, 2018: Human Pathology
Xinqiang Huang, Li Li, Ron Ammar, Yan Zhang, Yihe Wang, Kandasamy Ravi, John Ryan Thompson, Gabor Jarai
The translation of novel pulmonary fibrosis therapies from preclinical models into the clinic represents a major challenge demonstrated by the high attrition rate of compounds that showed efficacy in preclinical models but demonstrated no significant beneficial effects in clinical trials. Precision-cut lung tissue slice (PCLS) contains all major cell types of the lung and preserves the original cell-cell and cell-matrix contacts. It represents a promising ex vivo model to study pulmonary fibrosis. In this study, using RNA sequencing, we demonstrated that TGFβ1 induced robust fibrotic responses in the rat PCLS model as it changed the expression of genes functionally related to extracellular matrix remodeling, cell adhesion, epithelial-to-mesenchymal transition and various immune responses...
November 29, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
Lili Guo, Cencen Sun, Shilei Xu, Yue Xu, Qiuping Dong, Linlin Zhang, Wei Li, Xingyu Wang, Guoguang Ying, Fengjie Guo
BACKGROUND: The main cause of death in patients with non-small cell lung cancer (NSCLC) is the progression of cancer metastasis, which can be attributed to multiple factors, such as cancer stem cells (CSCs) and epithelial-mesenchymal transition (EMT). Long non-coding RNAs (lncRNAs) play important roles in the regulation of the cell cycle, cell proliferation, immune responses, and metastasis in cancers, but the potential roles and mechanisms of lincRNAs in CSC-like properties of cancer have not yet been elucidated...
November 28, 2018: Thoracic Cancer
Han Wu, Hai Yan Liu, Wen Jie Liu, Yong Li Shi, Dawei Bao
Lung carcinoma is the most common type of malignant tumors globally, and its molecular mechanisms remained unclear. With the aim to investigate the effects of microRNA (miR)-377-5p on the cell development, invasion, metastasis, and cycle of lung carcinoma, this study was performed. We evaluated miR-377-5p expression levels in lung cancer tissues and cell models. Cell viability, proliferation, migration, invasion abilities, and cell cycle distribution were measured using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, crystal violet, transwell, and flow cytometry assay...
November 28, 2018: Journal of Cellular Biochemistry
Xiaochen Zhang, Liyuan Wei, Jun Li, Jing Zheng, Shirong Zhang, Jianying Zhou
Circulating tumor cells (CTCs) are closely associated with cancer metastasis in preclinical models and patients with cancer. However, to the best of the authors knowledge, it remains unknown which type of CTCs may serve the key role in cancer metastasis. The present study investigated the association between the epithelial‑mesenchymal transition (EMT) phenotype of CTCs from the peripheral blood and distant metastasis in patients with non‑small cell lung cancer (NSCLC). Expression of EMT markers in CTCs from a cohort of patients was detected using Canpatrol™ CTC assays...
November 21, 2018: Molecular Medicine Reports
Wenlin Yang, Ying Su, Chenjian Hou, Linlin Chen, Danmei Zhou, Kehan Ren, Zhaoping Zhou, Renya Zhang, Xiuping Liu
SET domain bifurcated 1 (SETDB1) is a histone H3 lysine 9 methyltransferase that is highly expressed in various tumor types, including breast cancer. However, how SETDB1 functions in breast cancer is unclear. In the present study, proliferation, migration and invasion assays were performed to explore the role of SETDB1 in breast cancer cells. SETDB1 downregulation in BT549 and MDA‑MB‑231 cells reduced cell proliferation, whereas upregulation in MCF7 and T47D cells enhanced proliferation. Depletion of SETDB1 suppressed cell migration and invasion in vitro and reduced lung metastasis in vivo...
November 19, 2018: Oncology Reports
Jin-Feng Zhu, Yi Liu, He Huang, Li Shan, Zhi-Gang Han, Jun-Yuan Liu, Ying-Long Li, Xiang Dong, Wei Zeng
Background: Anoikis resistance has been demonstrated to facilitate distant metastases of cancers. MicroRNA-133b (miR-133b) is found to be down-regulated in various tumors, including esophageal squamous cell carcinoma (ESCC), and closely correlates with the malignant phenotype of ESCC. This study aimed to evaluate the roles of miR-133b in metastases of ESCC via regulating anoikis. Methods: The expression of miR-133b and related molecules were detected in ESCC tissues and cells...
2018: Cancer Cell International
Toni Celià-Terrassa, Caleb Bastian, Daniel Liu, Brian Ell, Nicole M Aiello, Yong Wei, Jose Zamalloa, Andres M Blanco, Xiang Hang, Dmitriy Kunisky, Wenyang Li, Elizabeth D Williams, Herschel Rabitz, Yibin Kang
Epithelial-mesenchymal transition (EMT) have been extensively characterized in development and cancer, and its dynamics have been modeled as a non-linear process. However, less is known about how such dynamics may affect its biological impact. Here, we use mathematical modeling and experimental analysis of the TGF-β-induced EMT to reveal a non-linear hysteretic response of E-cadherin repression tightly controlled by the strength of the miR-200s/ZEBs negative feedback loop. Hysteretic EMT conveys memory state, ensures rapid and robust cellular response and enables EMT to persist long after withdrawal of stimuli...
November 27, 2018: Nature Communications
Kai Liu, Shao-Hua Xu, Zhao Chen, Qing-Xin Zeng, Zhi-Jun Li, Zhou-Miao Chen
BACKGROUND: Waixenicin A, a bioactive extract of soft coral Sarcothelia edmondsoni, has been shown to be anti-neoplastic. However, its mechanisms of action remain unclear. Cancer stem cells (CSCs) and associated stemness factors are implicated in lung cancer. Here, we investigated the role of Waixenicin A on CSCs-like and metastatic lung cancer cells. METHODS: We demonstrated and compared TRPM7 expression in the non-tumor lung tissues or bronchial epithelial 16-HBE cell line...
November 26, 2018: BMC Cancer
Rui-Lian Chen, Jun Zhao, Xu-Chao Zhang, Na-Na Lou, Hua-Jun Chen, Xue Yang, Jian Su, Zhi Xie, Qing Zhou, Hai-Yan Tu, Wen-Zhao Zhong, Hong-Hong Yan, Wei-Bang Guo, Yi-Long Wu, Jin-Ji Yang
OBJECTIVE: Crizotinib can target against mesenchymal-epithelial transition (MET) and anaplastic lymphoma kinase (ALK), which has been considered as a multi-targeted tyrosine kinase inhibitor (TKI). The objective of this study was to explore the efficacy of crizotinib in advanced non-small-cell lung cancer (NSCLC) with concomitant ALK rearrangement and c-Met overexpression. METHODS: Totally, 4622 advanced NSCLC patients from two institutes (3762 patients at the Guangdong Lung Cancer Institute from January 2011 to December 2016 and 860 cases at the Perking Cancer Hospital from January 2015 to December 2016) were screened for ALK rearrangement with any method of IHC, RACE-coupled PCR or FISH...
November 26, 2018: BMC Cancer
Wei Yang, Zhiming Shan, Xinfang Zhou, Liangqun Peng, Chongyang Zhi, Junhui Chai, Hongxing Liu, Junmei Yang, Zhandong Zhang
OBJECTIVE: Osteosarcoma is the most common primary malignant skeleton tumor that derives from mesenchymal cells. Emerging evidences have identified the vital role of long non-coding RNAs (lncRNAs) in the development of osteosarcoma. In this study, we aimed to investigate the role of lncRNA gastric carcinoma highly expressed transcript 1 (GHET1) in osteosarcoma progression. METHODS: The expression levels of relevant genes in clinical samples and cell lines were determined by quantitative real-time PCR...
November 8, 2018: Cancer Biomarkers: Section A of Disease Markers
Cao Bin, He Xiaofeng, Xu Wanzi
PURPOSE: To investigate the effect of microRNA-129 on the migration and invasion in (Non small cell lung cancer) NSCLC cells and involved mechanism. MATERIALS AND METHODS: A549 cells were cultured and transfected with microRNA-129 (miR-129) mimic. Wound-healing assay and transwell assay were used to analyses the migration and invasion of A549 cells, respectively. The SOX4 expression changes were detected by qRT-PCR and Western blot. Dual-luciferase reporter assay was used to determine the binding site between miR-129 and the 3'-UTR of SOX4...
November 26, 2018: Experimental Lung Research
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