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https://www.readbyqxmd.com/read/28927010/the-role-of-oocyte-organelles-in-determining-developmental-competence
#1
REVIEW
Karen L Reader, Jo-Ann L Stanton, Jennifer L Juengel
The ability of an oocyte to undergo successful cytoplasmic and nuclear maturation, fertilization and embryo development is referred to as the oocyte's quality or developmental competence. Quality is dependent on the accumulation of organelles, metabolites and maternal RNAs during the growth and maturation of the oocyte. Various models of good and poor oocyte quality have been used to understand the essential contributors to developmental success. This review covers the current knowledge of how oocyte organelle quantity, distribution and morphology differ between good and poor quality oocytes...
September 18, 2017: Biology
https://www.readbyqxmd.com/read/28925797/transcription-driven-dna-methylation-setting-on-the-mouse-peg3-locus
#2
Corey L Bretz, Joomyeong Kim
The imprinting of the mouse Peg3 domain is controlled through the Peg3-DMR, which obtains its maternal-specific DNA methylation during oogenesis. In the current study, we deleted an oocyte-specific alternative promoter, termed U1, which is localized 20 kb upstream of the Peg3-DMR. Deletion of this alternative promoter resulted in complete removal of the maternal-specific DNA methylation on the Peg3-DMR. Consequently, the imprinted genes in the Peg3 domain become biallelic in the mutants with maternal transmission of the deletion...
September 19, 2017: Epigenetics: Official Journal of the DNA Methylation Society
https://www.readbyqxmd.com/read/28925272/-fertility-preserving-methods-in-women-with-breast-cancer-before-gonadotoxic-therapy
#3
N Halászová, I Crha, M Huser, V Weinberger, J Žáková, M Ješeta, E Lousová, E Filipinská, P Ventruba
OBJECTIVE: To present current options and own experiences with ovarian stimulation in young women with breast cancer before gonadotoxic therapy. DESIGN: Review article with own experiencies. METHODS: Literary search, analysis of own experiences with complex therapy in women with breast cancer planning pregnancy, definitions of stimulating protocols, using methods of assisted reproduction. SETTING: Department of Obstetrics and Gynecology, Faculty of Medicine, Masaryk University and University Hospital Brno...
2017: Ceská Gynekologie
https://www.readbyqxmd.com/read/28923708/enhanced-in%C3%A2-vitro-maturation-of-canine-oocytes-by-oviduct-epithelial-cell-co-culture
#4
Jingu No, Minghui Zhao, Seunghoon Lee, Sun A Ock, Yoonseok Nam, Tai-Young Hur
Canine-assisted reproductive techniques have been successful for several years; however, the lack of an oocyte in vitro maturation system has limited their application. The aim of this study was to evaluate the effect of canine oviduct epithelial cells (cOECs) on canine oocyte maturation in vitro. Specifically, the method used for isolation of cOECs did not affect the expression of epithelial markers, E-cadherin and cytokeratin, on fresh, cultured and cryopreserved cells. Moreover, BrdU analysis showed that cOECs cultured in Medium 171 supplemented with mammary epithelial growth supplement were more proliferative than counterparts in advanced Dulbecco's modified Eagle medium or Medium 199...
September 6, 2017: Theriogenology
https://www.readbyqxmd.com/read/28923703/transcriptome-analysis-of-three-critical-periods-of-ovarian-development-in-yellow-river-carp-cyprinus-carpio
#5
Yongfang Jia, Ping Nan, Wanwan Zhang, Fang Wang, Ruihua Zhang, Tingting Liang, Xiaolin Ji, Qiyan Du, Zhongjie Chang
Ovary development is a complex process involving numerous genes; the molecular mechanism underlying the ovary development of carp is still unknown. Here we used Illumina HiSeq™ 2500 to explore the transcriptome of undifferentiated gland (PG), juvenile ovary (OJ) and adult ovary (OA) of Yellow River carp (Cyprinus carpio). A total of 58,749 unigenes were obtained, comprising 45,707 known genes and 13,042 new genes. We identified differentially-expressed genes (DEGs) during development and characterized the functional properties of DEGs by comparison with the gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes databases...
September 9, 2017: Theriogenology
https://www.readbyqxmd.com/read/28923420/high-doses-of-lipid-core-nanocapsules-do-not-affect-bovine-embryonic-development-in-vitro
#6
Caroline Lucas, Mariana Remião, Franciele Bruinsmann, Isadora Lopes, Morgana Borges, Ana Laura Feijó, Andrea Cristina Basso, Adriana Pohlmann, Silvia Guterres, Vinicius Campos, Fabiana Seixas, Tiago Collares
The improvement of in vitro embryo production by culture media supplementation has been a potential tool to increase blastocyst quality and development. Recently, lipid-core nanocapsules (LNC), which were developed for biomedical applications as a drug-delivery system, have demonstrated beneficial effects on in vitro embryo production studies. LNCs have a core composed of sorbitan monostearate dispersed in capric/caprylic triglyceride. Based on that, we firstly investigated if LNCs supplemented during in vitro oocyte maturation had affinity to the mineral oil placed over the top of the IVM media...
September 15, 2017: Toxicology in Vitro: An International Journal Published in Association with BIBRA
https://www.readbyqxmd.com/read/28923286/abnormally-fertilized-oocytes-can-result-in-healthy-live-births-improved-genetic-technologies-for-preimplantation-genetic-testing-can-be-used-to-rescue-viable-embryos-in-in-vitro-fertilization-cycles
#7
Antonio Capalbo, Nathan Treff, Danilo Cimadomo, Xin Tao, Susanna Ferrero, Alberto Vaiarelli, Silvia Colamaria, Roberta Maggiulli, Giovanna Orlando, Catello Scarica, Richard Scott, Filippo Maria Ubaldi, Laura Rienzi
OBJECTIVE: To test whether abnormally fertilized oocyte (AFO)-derived blastocysts are diploid and can be rescued for clinical use. DESIGN: Longitudinal-cohort study from January 2015 to September 2016 involving IVF cycles with preimplantation genetic testing for aneuploidy (PGT-A). Ploidy assessment was incorporated whenever a blastocyst from a monopronuclear (1PN) or tripronuclear zygote (2PN + 1 smaller PN; 2.1 PN) was obtained. SETTING: Private IVF clinics and genetics laboratories...
September 15, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28923285/patient-and-cycle-characteristics-predicting-high-pregnancy-rates-with-single-embryo-transfer-an-analysis-of-the-society-for-assisted-reproductive-technology-outcomes-between-2004-and-2013
#8
Jennifer Mersereau, Jamie Stanhiser, Charles Coddington, Tiffany Jones, Barbara Luke, Morton B Brown
OBJECTIVE: To analyze factors associated with high live birth rate and low multiple birth rate in fresh and frozen-thawed assisted reproductive technology (ART) cycles. DESIGN: Retrospective cohort analysis. SETTING: Not applicable. PATIENT(S): The study population included 181,523 women undergoing in vitro fertilization with autologous fresh first cycles, 27,033 with fresh first oocyte donor cycles, 37,658 with fresh second cycles, and 35,446 with frozen-thawed second cycles...
September 15, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28922750/identification-of-iridoid-glucoside-transporters-in-catharanthus-roseus
#9
Bo Larsen, Victoria L Fuller, Jacob Pollier, Alex Van Moerkercke, Fabian Schweizer, Richard Payne, Maite Colinas, Sarah E O'Connor, Alain Goossens, Barbara A Halkier
Monoterpenoid indole alkaloids (MIAs) are plant defense compounds and high-value pharmaceuticals. Biosynthesis of the universal MIA precursor, secologanin, is organized between internal phloem-associated parenchyma (IPAP) and epidermis cells. Transporters for intercellular transport of proposed mobile pathway intermediates have remained elusive. Screening of an Arabidopsis thaliana transporter library expressed in Xenopus oocytes identified AtNPF2.9 as a putative iridoid glucoside importer. Eight orthologs were identified in Catharanthus roseus, of which three, CrNPF2...
September 1, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28922408/the-role-of-the-pi3k-akt-signaling-pathway-in-the-developmental-competence-of-bovine-oocytes
#10
Gabriella Mamede Andrade, Juliano Coelho da Silveira, Claudia Perrini, Maite Del Collado, Samuel Gebremedhn, Dawit Tesfaye, Flávio Vieira Meirelles, Felipe Perecin
The ovarian follicle encloses oocytes in a microenvironment throughout their growth and acquisition of competence. Evidence suggests a dynamic interplay among follicular cells and oocytes, since they are constantly exchanging "messages". We dissected bovine ovarian follicles and recovered follicular cells (FCs-granulosa and cumulus cells) and cumulus-oocyte complexes (COCs) to investigate whether the PI3K-Akt signaling pathway impacted oocyte quality. Following follicle rupture, COCs were individually selected for in vitro cultures to track the follicular cells based on oocyte competence to reach the blastocyst stage after parthenogenetic activation...
2017: PloS One
https://www.readbyqxmd.com/read/28921586/down-syndrome-maternal-serum-markers-in-oocyte-donation-and-other-assisted-reproductive-technologies
#11
Aurore Bonnin, Françoise Muller, Marie-Victoire Senat, Corinne Sault, Armelle Galland, Joëlle Taieb, Sophie Dreux, Jean Bouyer, Alexandra Benachi
OBJECTIVE: Because maternal serum markers (PAPP-A, hCGβ, and AFP) used for Down syndrome (DS) screening have been described as predictors of obstetrical complications and because assisted reproductive technology (ART) pregnancies are known to be at increased risk for obstetrical complications, it is unclear whether or not correction factors should be applied to the calculated risk of DS. The purpose of this study was to evaluate DS maternal serum markers in oocyte donation (OD) and ART pregnancies in comparison with natural pregnancies...
September 16, 2017: Prenatal Diagnosis
https://www.readbyqxmd.com/read/28920560/indirect-evidence-of-pathogen-associated-altered-oocyte-production-in-queens-of-the-invasive-yellow-crazy-ant-anoplolepis-gracilipes-in-arnhem-land-australia
#12
M D Cooling, B D Hoffmann, M A M Gruber, P J Lester
Anoplolepis gracilipes is one of the six most widespread and pestiferous invasive ant species. Populations of this invader in Arnhem Land, Australia have been observed to decline, but the reasons behind these declines are not known. We investigated if there is evidence of a pathogen that could be responsible for killing ant queens or affecting their reproductive output. We measured queen number per nest, fecundity and fat content of queens from A. gracilipes populations in various stages of decline or expansion...
September 18, 2017: Bulletin of Entomological Research
https://www.readbyqxmd.com/read/28920413/functional-role-of-forskolin-and-pd166285-in-the-development-of-denuded-mouse-oocytes
#13
Hongguo Cao, Yani Bian, Fei Zhang, Yunshu Tang, Caixia Li, Jiemei Chen, Xiaorong Zhang
Objective: cAMP and MPF play critical roles during the maturation of mammalian oocytes. The aim of this study was to produce the offspring from denuded oocytes in mice by regulating cAMP and MPF. Methods: In this study, we used DOs at the germinal vesicle (GV) stage in mice and regulated levels of cAMP and MPF in DOs by adding Forskolin and PD166285 during in vitro maturation (IVM) without FSH and LH respectively. Results: Combined use of 50 μM Forskolin for 3 h and 2...
September 18, 2017: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/28919864/%C3%AE-subunits-control-the-effects-of-human-kv4-3-potassium-channel-phosphorylation
#14
Geoffrey W Abbott
The transient outward K(+) current, Ito, activates early in the cardiac myocyte action potential, to begin repolarization. Human Ito is generated primarily by two Kv4.3 potassium channel α subunit splice variants (Kv4.3L and Kv4.3S) that diverge only by a C-terminal, membrane-proximal, 19-residue stretch unique to Kv4.3L. Protein kinase C (PKC) phosphorylation of threonine 504 within the Kv4.3L-specific 19-residues mediates α-adrenergic inhibition of Ito in human heart. Kv4.3 is regulated in human heart by various β subunits, including cytosolic KChIP2b and transmembrane KCNEs, yet their impact on the functional effects of human Kv4...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28919301/prostaglandins-in-teleost-ovulation-a-review-of-the-roles-with-a-view-to-comparison-with-prostaglandins-in-mammalian-ovulation
#15
REVIEW
Takayuki Takahashi, Akane Hagiwara, Katsueki Ogiwara
Prostaglandins are well known to be central regulators of vertebrate ovulation. Studies addressing the role of prostaglandins in mammalian ovulation have established that they are involved in the processes of oocyte maturation and cumulus oocyte complex expansion. In contrast, despite the first indication of the role of prostaglandins in teleost ovulation appearing 40 years ago, the mechanistic background of their role has long been unknown. However, studies conducted on medaka over the past decade have provided valuable information...
September 15, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28919178/the-presence-of-abalone-egg-laying-hormone-like-peptide-in-the-central-nervous-system-and-ovary-of-the-spotted-babylon-babylonia-areolata
#16
Jirawat Saetan, Piyakorn Boonyoung, Uraporn Vongvatcharanon, Thanapong Kruangkum, Kanjana Khornchatri, Pinij Thaweethamsewee, Prasert Sobhon, Prapee Sretarugsa
Recently, the neuronal classification of the ivory shell Spotted Babylon, Babylonia areolata, was readily demonstrated. Regarding its importance as marine economic molluscan species, the attempt to understand the neuroendocrine regulation is necessary. This study firstly demonstrated the neurosecretory cells as well as the existence and distribution of the egg-laying hormone (ELH)-like peptide in the central nervous system (CNS) and ovary of the B. areolata. The neurosecretory cell was characterized by the cytoplasmic purple dot-like structure as stained by the Gomori's paraldehyde fuchsin...
September 14, 2017: Acta Histochemica
https://www.readbyqxmd.com/read/28917942/in-vivo-and-in-vitro-testing-of-native-%C3%AE-conotoxins-from-the-injected-venom-of-conus-purpurascens
#17
REVIEW
Mickelene F Hoggard, Alena M Rodriguez, Herminsul Cano, Evan Clark, Han-Shen Tae, David J Adams, Tanja A Godenschwege, Frank Marí
α-Conotoxins inhibit nicotinic acetylcholine receptors (nAChRs) and are used as probes to study cholinergic pathways in vertebrates. Model organisms, such as Drosophila melanogaster, express nAChRs in their CNS that are suitable to investigate the neuropharmacology of α-conotoxins in vivo. Here we report the paired nanoinjection of native α-conotoxin PIA and two novel α-conotoxins, PIC and PIC[O7], from the injected venom of Conus purpurascens and electrophysiological recordings of their effects on the giant fiber system (GFS) of D...
September 13, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28917093/molecular-cloning-and-functional-expression-of-the-k-channel-kv7-1-and-the-regulatory-subunit-kcne1-from-equine-myocardium
#18
Philip J Pedersen, Kirsten B Thomsen, Jon B Flak, Maria A Tejada, Frank Hauser, Dagmar Trachsel, Rikke Buhl, Theodore Kalbfleisch, Michael Scott DePriest, James N MacLeod, Kirstine Calloe, Dan A Klaerke
BACKGROUND: The voltage-gated K(+)-channel KV7.1 and the subunit KCNE1, encoded by the KCNQ1 and KCNE1 genes, respectively, are responsible for termination of the cardiac action potential. In humans, mutations in these genes can predispose patients to arrhythmias and sudden cardiac death (SCD). AIM: To characterize equine KV7.1/KCNE1 currents and compare them to human KV7.1/KCNE1 currents to determine whether KV7.1/KCNE1 plays a similar role in equine and human hearts...
September 11, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28916873/effects-of-methotrexate-on-the-quality-of-oocyte-maturation-in-vitro
#19
N Tian, J Yu, Sh Zhang, W Y Ma, T Wang, Y M Wang
Methotrexate (MTX), an antifolate drug, is widely used for clinical treatment of malignancies and ectopic pregnancy. Many studies have documented that MTX has strong side-effects on rapidly dividing somatic cells. However, its side-effects on female reproductive cells have not been widely reported. Combined with in vitro culture, two-photon fluorescence imaging and three-dimensional reconstruction, this study analyzed the effects of MTX on oocyte maturation time, chromosome arrangement, karyotype, spindle morphology, and the localization of microtubule organizing centers (MTOCs)...
September 15, 2017: European Biophysics Journal: EBJ
https://www.readbyqxmd.com/read/28916758/role-of-the-venus-kinase-receptor-in-the-female-reproductive-physiology-of-the-desert-locust-schistocerca-gregaria
#20
Cynthia Lenaerts, Jolien Palmans, Elisabeth Marchal, Rik Verdonck, Jozef Vanden Broeck
Venus kinase receptors (VKR) are a subfamily of invertebrate receptor tyrosine kinases, which have only recently been discovered. They contain an intracellular tyrosine kinase domain and an extracellular Venus FlyTrap domain. VKRs have been functionally and pharmacologically characterized in only two invertebrate species, namely the human parasite Schistosoma mansoni and the mosquito Aedes aegypti, where they play a crucial role in oogenesis. Here, we report the characterization of a VKR in the desert locust, Schistocerca gregaria...
September 15, 2017: Scientific Reports
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