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Tanja N Fehm, Franziska Meier-Stiegen, Christiane Driemel, Bernadette Jäger, Florian Reinhardt, Johanna Naskou, André Franken, Hans Neubauer, Rui P L Neves, Guus van Dalum, Eugen Ruckhäberle, Dieter Niederacher, Jutta M Rox, Johannes C Fischer, Nikolas H Stoecklein
Diagnostic leukapheresis (DLA) is based on continuous centrifugation that collects mononuclear cells from peripheral blood with a density of 1.055-1.08 g/ml. As epithelial cells have a similar density, DLA cocollects circulating tumor cell (CTCs) along with the targeted mononuclear cells. Here, we report on our single center experience applying DLA in 40 nonmetastatic and metastatic breast cancer patients and its impact on CTC detection. We found that the use of just 5% of the DLA product (corresponding to a median peripheral blood volume of around 60 ml) in the CellSearch® assay already leads to a significant increase in CTC detection frequency and yield...
December 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
Mariano Di Trapani, Nicolò Manaresi, Gianni Medoro
Circulating tumor cells (CTCs) are rare cells shed into the bloodstream by invasive tumors and their analysis offers a promising noninvasive tool to predict and monitor therapeutic responses. CTCs can be isolated from patient blood and their characterization at single-cell level can inform on the genomic landscape of a tumor. All CTC enrichment methods bear a burden of contaminating normal cells, which mandate a further step of purification to enable reliable downstream genetic analysis. Here, we describe the DEPArray™ technology, a microchip-based digital sorter, which combines precise microfluidic and microelectronic enabling precise, image-based isolation of single CTCs, which can then be analyzed by Next Generation Sequencing (NGS) methods...
December 2018: Cytometry. Part A: the Journal of the International Society for Analytical Cytology
Ayana Okamoto, Kosuke Minaga, Mamoru Takenaka, Tomoe Yoshikawa, Toshimitsu Iwasaki, Masakatsu Tsurusaki, Masatoshi Kudo
No abstract text is available yet for this article.
December 14, 2018: Endoscopy
Martin Freesmeyer, Wolfgang Weigand, Thomas Weisheit
AIM: In this contribution we investigated the potential transfer of an established method for the synthesis of 223 Ra-labelled calixarene complexes to a hospital radiopharmaceutical environment. For this purpose, commercially available [223 Ra]RaCl2 solution in pharmaceutical grade (Xofigo® ) was reacted with three calixarene derivatives. METHODS: A wellestablished two-phase extraction method using a two-phase solvent system was performed for complexation of the radium ions with calixarenes under slightly basic conditions...
December 2018: Nuklearmedizin. Nuclear Medicine
Yurika Fujita, Hiroshi Honda, Masayuki Yamane, Takeshi Morita, Tomonari Matsuda, Osamu Morita
Genotoxicity evaluation has been widely used to estimate the carcinogenicity of test substances during safety evaluation. However, the latest strategies using genotoxicity tests give more weight to sensitivity; therefore, their accuracy has been very low. For precise carcinogenicity evaluation, we attempted to establish an integrated testing strategy for the tailor-made carcinogenicity evaluation of test materials, considering the relationships among genotoxicity test results (Ames, in vitro mammalian genotoxicity and in vivo micronucleus), carcinogenicity test results and chemical properties (molecular weight, logKow and 179 organic functional groups)...
December 14, 2018: Mutagenesis
Jérôme Lavoué, Lawrence Joseph, Peter Knott, Hugh Davies, France Labrèche, Frédéric Clerc, Gautier Mater, Tracy Kirkham
Introduction: Interpretation of exposure measurements has evolved into a framework based on the lognormal distribution. Most available practical tools are based on traditional frequentist statistical procedures that do not satisfactorily account for censored data and are not amenable to simple probabilistic risk statements. Bayesian methods offer promising solutions to these challenges. Such methods have been proposed in the literature but are not widely and freely available to practitioners...
December 14, 2018: Annals of Work Exposures and Health
Donatella Paoli, Giulia Pecora, Francesco Pallotti, Fabiana Faja, Marianna Pelloni, Andrea Lenzi, Francesco Lombardo
STUDY QUESTION: Is ageing associated with a decline in semen quality and molecular changes to human sperm? SUMMARY ANSWER: Semen quality declines with advancing age and characteristic molecular changes take place during the ageing process, including increased sperm DNA damage, altered sperm protamination and altered seminal plasma miRNA profile. WHAT IS KNOWN ALREADY: During ageing, the reproductive system is exposed to physiological changes and potentially damaging factors that may impair testicular function...
December 14, 2018: Human Reproduction
W Mengesha, R Steege, A Z Kea, S Theobald, D G Datiko
Background: Health extension workers (HEWs) are the key cadre within the Ethiopian Health Extension Programme extending health care to rural communities. National policy guidance supports the use of mHealth to improve data quality and use. We report on a mobile Health Management Information system (HMIS) with HEWs and assess its impact on data use, community health service provision and HEWs' experiences. Methodology: We used a mixed methods approach, including an iterative process of intervention development for 2 out of 16 essential packages of health services, quantitative analysis of new registrations, and qualitative research with HEWs and their supervisors...
December 1, 2018: Journal of Public Health
Lin-Xia Tang, Bin Wang, Zhi-Kun Wu
BACKGROUND Diabetic nephropathy was one of the most serious and harmful diabetic complications, characterized by progressive loss of renal function and renal fibrosis. Aerobic exercise training is an important non-pharmacologic method to prevent and treat diabetes mellitus and diabetic complications. MATERIAL AND METHODS Intraperitoneal (i.p.) injection of streptozocin (STZ) was used to construct a type 1 diabetic mouse model. Renal function and mitochondrial function were measured by urinary protein level, Masson staining and ATP, superoxide production, and membrane potential, respectively...
December 14, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Andrea Hams, Kerrie Evans, Roger Adams, Gordon Waddington, Jeremy Witchalls
OBJECTIVE: Investigate the patterns and circumstances of shoulder injury, in both male and female sub-elite water polo players, through evaluating the injury incidence, mechanism and subsequent training time lost. DESIGN: Retrospective cohort. SETTING: Sports institute. PARTICIPANTS: 80 sub-elite water polo players. MAIN OUTCOME MEASURES: Total injury number and incidence, mechanism of injury, lost training time and time from injury onset to seeking treatment...
December 5, 2018: Physical Therapy in Sport
Joanna Nizioł, Maria Misiorek, Tomasz Ruman
Strawberry (Fragaria x ananassa Duch., Rosaceae) is the subject of many research studies due to its numerous features such as unique taste, aroma and health qualities. The distribution of low molecular weight metabolites belonging to aldehydes, ketones, alcohols, esters, organic acids, phenolics, amino acids and sugars classes within strawberry fruit cross-section was studied using mass spectrometry imaging (MSI) method with 109 Ag nanoparticle enhanced target (109 AgNPET). Correlation of distribution of over thirty compounds found in cross-section of strawberry with their biological function is also included...
December 11, 2018: Phytochemistry
Kai He, Ming Yan, Zhenzhen Huang, Guangming Zeng, Anwei Chen, Tiantian Huang, Hui Li, Xiaoya Ren, Guiqiu Chen
In this work, silver nanoparticles supported on polydopamine-kaolin composite (PDA-kaolin-Ag) was fabricated by an in-situ reduction method with PDA as both reductant and stabilizer. The morphology, composition, and structure of PDA-kaolin-Ag composite were characterized by transmission electron microscopy (TEM), X-ray powder diffraction (XRD), and X-ray photoelectron spectroscopy (XPS). The catalytic reduction tests confirmed the importance of PDA modification and high catalytic activities of this as-prepared PDA-kaolin-Ag composite towards a model dye Rhodamine B...
December 7, 2018: Chemosphere
Jianqing Chen, Yanmin Xu, Qi Han, Yuchang Yao, Houjuan Xing, Xiaohua Teng
Cadmium (Cd), a hazardous environmental contaminant with irreversible toxicity to fish, has been detected in aquatic environment of many countries. The common carp is one of the most widely distributed fish in the world, so we used common carp to assess environmental contaminant risk. In present study, we investigated effects of Cd on immune function, oxidative defense, and glycometabolism in the spleens of common carp by transcriptome analysis. Obtained 3794 differentially expressed genes (including 1848 up-regulated and 1946 down-regulated genes) were enriched using databases of Kyoto Encyclopedia of Genes and Genomes, and Gene Ontology in David bioinformatics software (version 6...
December 5, 2018: Journal of Hazardous Materials
F Ruatta, L Derosa, B Escudier, E Colomba, A Guida, G Baciarello, Y Loriot, K Fizazi, L Albiges
BACKGROUND: Bone metastases (BMs) are associated with significant morbidity and shorter survival in renal cell carcinoma (RCC). Our purpose was to identify prognostic factors for overall survival (OS) in RCC patients with BMs. METHODS: Data from patients with BMs from RCC treated at Gustave Roussy between April 1992 and March 2016 were retrospectively collected. Age, sex, Eastern Cooperative Oncology Group-Performance Status, Memorial Sloan-Kettering Cancer Center (MSKCC) risk groups, histology, number and site of bone lesions, concomitant metastases (presence and sites), therapy for BMs (radical resection or palliative surgery, radiotherapy and other local and systemic treatments) and time from diagnosis to BMs were analysed...
December 11, 2018: European Journal of Cancer
Lara Saftić, Željka Peršurić, Emilia Fornal, Tomislav Pavlešić, Sandra Kraljević Pavelić
Propolis is a complex biological matrix consisting mostly of plant resins and waxes, and in a small proportion of the herbal secondary metabolites, phenols. Phenols are components that are responsible for biological activities of propolis, however, their qualitative and quantitative composition is strongly influenced by climate and vegetation. Although studies on profiling of propolis samples from different countries have been carried out for some time propolis from Croatia is still not characterized till now...
December 4, 2018: Journal of Pharmaceutical and Biomedical Analysis
Orlando Marin, Maria Alesker, Shani Guttman, Gregory Gershinsky, Eitan Edri, Hagay Shpaisman, Rodrigo E Guerra, David Zitoun, Moshe Deutsch, Eli Sloutskin
HYPOTHESIS: Temperature-controlled self-faceting of liquid droplets has been recently discovered in surfactant-stabilized alkane-in-water emulsions. We hypothesize that similar self-faceting may occur in emulsion droplets of UV-polymerizable linear hydrocarbons. We further hypothesize that the faceted droplet shapes can be fixed by UV-initiated polymerization, thus providing a new route towards the production of solid polyhedra. EXPERIMENTS: Temperature-induced shape variations were studied by optical microscopy in micron-size emulsion droplets of UV-polymerizable alkyl acrylate...
December 2, 2018: Journal of Colloid and Interface Science
Khaled O Mohamed, Islam Zaki, Ibrahim M El-Deen, Mohammed K Abdelhameid
A new series of diamide functional compounds has been designed, synthesized and confirmed by spectroscopic methods and elemental analyses. All the synthesized compounds were evaluated for their antiproliferative activity on HepG2 cell line. Compounds 3k and 3l were proved to have potent anticancer activity equipotent or more potent than reference compound Combretastatin A-4. The results of DNA flow cytometry analysis demonstrated cell cycle arrest at G2/M phase. The extent of apoptosis induced by 3k and 3l was also determined...
December 5, 2018: Bioorganic Chemistry
Na Sai, Zhong Sun, Yuntang Wu, Guowei Huang
In this study, a easy-to-prepare biosensor for the sensitive detection of the antibody (Ab) protein was developed using a novel microgel photonic crystal (MPC). The MPC was fabricated by the spin-coated self-assembly method with the monodisperse Ab-sensitive poly (methyl methacrylate-acrylamide-glutaraldehyde-hapten) (P(MMA-AM-GA-HP)) microgels. Morphology characterization showed that the P(MMA-AM-GA-HP) microgels possessed round shapes and the large specific surface area, and the formed MPC had a highly ordered three dimensional (3D) periodically-ordered structure with the desired structural color...
December 5, 2018: Bioorganic Chemistry
C S Pundir, Parveen Kumar, Ranjana Jaiwal
Creatinine is a metabolic product of creatine phosphate in muscles, which provides energy to muscle tissues. Creatinine has been considered as indicator of renal function specifically after dialysis, thyroid malfunction and muscle damage. The normal level of creatinine in the serum and its excretion through urine in apparently healthy individuals is 45-140 μM and 0.8-2.0 gm/day respectively. The level of creatinine reaches >1000 μM in serum during renal, thyroid and kidney dysfunction or muscle disorder...
November 25, 2018: Biosensors & Bioelectronics
Margaret Fisher, Heather Bower, Samantha Chenery-Morris, Frances Galloway, Judith Jackson, Susan Way, Michael M Fisher
This paper presents the final phase of a national project exploring grading of practice in programmes leading to registration as a midwife in the United Kingdom. The aim was to develop a generic framework for grading practice, enhancing standardisation while enabling flexibility in application of current and new educational standards. A mixed method on-line survey considered existing practice assessment tools, factors contributing to robust and reliable assessment and perceptions of two assessment tools developed by the research team: a 'Lexicon Framework' and 'Rubric', which were tested through scenarios...
November 26, 2018: Nurse Education in Practice
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