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human adenovirus infection

Thomas Hiller, Johanna Berg, Laura Elomaa, Viola Röhrs, Imran Ullah, Katrin Schaar, Ann-Christin Dietrich, Munir A Al-Zeer, Andreas Kurtz, Andreas C Hocke, Stefan Hippenstiel, Henry Fechner, Marie Weinhart, Jens Kurreck
Bioprinting is a novel technology that may help to overcome limitations associated with two-dimensional (2D) cell cultures and animal experiments, as it allows the production of three-dimensional (3D) tissue models composed of human cells. The present study describes the optimization of a bioink composed of alginate, gelatin and human extracellular matrix (hECM) to print human HepaRG liver cells with a pneumatic extrusion printer. The resulting tissue model was tested for its suitability for the study of transduction by an adeno-associated virus (AAV) vector and infection with human adenovirus 5 (hAdV5)...
October 12, 2018: International Journal of Molecular Sciences
Li Min Lim, Yen Yen Woo, Jessie Anne de Bruyne, Anna Marie Nathan, Sze Ying Kee, Yoke Fun Chan, Chun Wei Chiam, Kah Peng Eg, Surendran Thavagnanam, I-Ching Sam
OBJECTIVES: To describe the severity, human adenovirus (HAdV) type and respiratory morbidity following adenovirus pneumonia in children. METHODOLOGY: Retrospective review of children under 12 years of age, admitted with HAdV pneumonia, between January 2011 and July 2013, in a single centre in Malaysia. HAdV isolated from nasopharyngeal secretions were typed by sequencing hypervariable regions 1-6 of the hexon gene. Patients were reviewed for respiratory complications...
2018: PloS One
Yongyun Li, Jie He, Chun Qiu, Qingfeng Shang, Guanxiang Qian, Xianqun Fan, Shengfang Ge, Renbing Jia
BACKGROUND: Uveal melanoma (UM) is a severe human malignancy with a high mortality rate, as well as high metastasis and recurrence potential. The active mutation of G protein subunit alpha q (GNAQ) or G protein subunit alpha 11 (GNA11) is a major trigger for UM. Oncolytic adenovirus H101 (H101) is the first oncolytic virus approved for clinical applications in cancer therapy by the China Food and Drug Administration. We investigated whether combining H101 with the downregulation of GNAQ expression would act synergistically in UM therapy...
October 15, 2018: Journal of Cellular Biochemistry
Stien Vandendriessche, Elizaveta Padalko, Elke Wollants, Charlotte Verfaillie, Bruno Verhasselt, Liselotte Coorevits
OBJECTIVES: The Seegene AllplexTM Respiratory panel was retrospectively challenged using a collection of quality control samples (QCMD) and clinical samples previously analysed with validated routine methods. METHODS: A collection of 111 samples [43 QCMD samples, 13 bronchoalveolar lavage fluids and 55 nasopharyngeal aspirates/swabs] was tested with Seegene AllplexTM . The clinical samples were tested previously using either FTD® Respiratory Pathogens 21 qPCR assay (Fast Track Diagnostics), an in-house multiplex PCR for Bordetella, or BioGX Sample-ReadyTM Atypical pneumo panel (Becton Dickinson)...
October 11, 2018: Acta Clinica Belgica
Kathy A Keith, Caroll B Hartline, Terry L Bowlin, Mark N Prichard
The search for new compounds with a broad spectrum of antiviral activity is important and requires the evaluation of many compounds against several distinct viruses. Researchers attempting to develop new antiviral therapies for DNA virus infections currently use a variety of cell lines, assay conditions and measurement methods to determine in vitro drug efficacy, making it difficult to compare results from within the same laboratory as well as between laboratories. In this paper, we describe the assessment of antiviral activity of a set of nucleoside analogs against BK polyomavirus, JC polyomavirus, Epstein-Barr virus, human herpesvirus 6B, and human herpesvirus 8 in an automated 384-well format and utilize qPCR assays to measure the accumulation of viral DNA...
October 1, 2018: Antiviral Research
Caroll B Hartline, Kathy A Keith, Jessica Eagar, Emma A Harden, Terry L Bowlin, Mark N Prichard
The search for new compounds with a broad spectrum of antiviral activity is important and requires the evaluation of many compounds against several distinct viruses. Researchers attempting to develop new antiviral therapies for DNA virus infections currently use a variety of cell lines, assay conditions and measurement methods to determine in vitro drug efficacy, making it difficult to compare results from within the same laboratory as well as between laboratories. In this paper we describe a common assay platform designed to facilitate the parallel evaluation of antiviral activity against herpes simplex virus type 1, herpes simplex virus type 2, varicella-zoster virus, cytomegalovirus, vaccinia virus, cowpox virus, and adenovirus...
October 1, 2018: Antiviral Research
Leyun Xie, Bing Zhang, Niguang Xiao, Fei Zhang, Xin Zhao, Qin Liu, Zhiping Xie, Hanchun Gao, Zhaojun Duan, Lili Zhong
To investigate the current genotypes of circulating HAdV strains, we molecularly genotyped HAdV in the nasopharyngeal aspirates (NPAs) of patients with acute lower respiratory tract infections (ALRTIs) and attempted to determine their associations with clinical symptoms. A total of 4751 nasopharyngeal aspirate (NPA) samples were collected from 4751 patients admitted to Hunan Provincial People's Hospital from September 2007 to March 2014, of which 447 (9.4%) samples were HAdV-positive. Fourteen different HAdV types were identified; HAdV types 1 to 7 (HAdV 1-7) were identified in 95...
October 4, 2018: Journal of Medical Virology
Sebastien Kenmoe, Marie-Astrid Vernet, Jerôme Le Goff, Véronique Beng Penlap, Astrid Vabret, Richard Njouom
BACKGROUND: Human adenoviruses (HAdVs) cause a wide range of diseases worldwide, including respiratory infections. Studies on HAdV molecular epidemiology are limited in Cameroon. The purpose of this study is to document the different types HAdV circulating in Cameroon in children with acute respiratory infections. METHODS: Nasopharyngeal swabs were collected from 811 children under 15 years from 2011 to 2014. The HAdV detection was assessed by semi-quantitative generic PCR r-gene®...
October 3, 2018: Virology Journal
Abhimanyu K Singh, Thanh H Nguyen, Márton Z Vidovszky, Balázs Harrach, Mária Benkő, Alan Kirwan, Lokesh Joshi, Michelle Kilcoyne, M Álvaro Berbis, F Javier Cañada, Jesús Jiménez-Barbero, Margarita Menéndez, Sarah S Wilson, Beth A Bromme, Jason G Smith, Mark J van Raaij
Murine adenovirus 2 (MAdV-2) infects cells of the mouse gastrointestinal tract. Like human adenoviruses, it is a member of the genus Mastadenovirus, family Adenoviridae. The MAdV-2 genome has a single fibre gene that expresses a 787 residue-long protein. Through analogy to other adenovirus fibre proteins, it is expected that the carboxy-terminal virus-distal head domain of the fibre is responsible for binding to the host cell, although the natural receptor is unknown. The putative head domain has little sequence identity to adenovirus fibres of known structure...
October 2, 2018: Journal of General Virology
Pierre M Jean Beltran, Katelyn C Cook, Yutaka Hashimoto, Cyril Galitzine, Laura A Murray, Olga Vitek, Ileana M Cristea
Viral proteins have evolved to target cellular organelles and usurp their functions for virus replication. Despite the knowledge of these critical functions for several organelles, little is known about peroxisomes during infection. Peroxisomes are primarily metabolic organelles with important functions in lipid metabolism. Here, we discovered that the enveloped viruses human cytomegalovirus (HCMV) and herpes simplex virus type 1 (HSV-1) induce the biogenesis of and unique morphological changes to peroxisomes to support their replication...
October 10, 2018: Cell Host & Microbe
Friedrich Hahn, Corina Hutterer, Christophe Henry, Stuart T Hamilton, Hanife Strojan, Alexandra Kraut, Ulrike Schulte, Martin Schütz, Stephan Kohrt, Christina Wangen, José Pfizer, Yohann Couté, William D Rawlinson, Stefan Strobl, Manfred Marschall
Human cytomegalovirus (HCMV) is a major human pathogen with seropositivity rates in the adult population ranging between 40% and 95%. HCMV infection is associated with severe pathology, such as life-threatening courses of infection in immunocompromised individuals and neonates. Current standard therapy with valganciclovir has the disadvantage of adverse side effects and viral drug resistance. A novel anti-HCMV drug, letermovir, has been approved recently, so that improved therapy options are available. Nevertheless, even more so far unexploited classes of compounds and molecular modes of action will be required for a next generation of antiherpesviral treatment strategies...
September 27, 2018: Antiviral Research
Ekramy E Sayedahmed, Rashmi Kumari, Shruti Shukla, Ahmed O Hassan, Sulma I Mohammed, Ian A York, Shivaprakash Gangappa, Suryaprakash Sambhara, Suresh K Mittal
There is a high incidence of adenovirus (AdV) infection in humans due to the presence of more than 60 types of human adenoviruses (HAdVs). The majority of individuals are exposed to one or more HAdV types early in their lives, leading to the development of AdV type-specific neutralizing antibodies. Similarly, immunization or gene therapy with AdV vectors leads to immune responses to the AdV vector. This 'vector immunity' is a concern for AdV vector-based applications for vaccines or gene therapy, especially when the repeated administration of a vector is required...
September 25, 2018: Vaccine
Samson S Y Wong, Cyril C Y Yip, Siddharth Sridhar, Kit-Hang Leung, Andrew K W Cheng, Ami M Y Fung, Ho-Yin Lam, Kwok-Hung Chan, Jasper F W Chan, Vincent C C Cheng, Bone S F Tang, Kwok-Yung Yuen
BACKGROUND: Human adenoviruses are common causes of community-acquired respiratory tract and enteric infections. Severe disseminated infections with high mortality rates may be seen in immunocompromised individuals. An accurate and cost-effective quantitative assay is essential not only for laboratory diagnosis of adenoviral infections, but also for monitoring of response to antiviral treatment. The diagnostic performance of an in-house quantitative polymerase chain reaction assay was compared to a commercial system...
September 27, 2018: Virology Journal
Anita Gola, Daniel Silman, Adam A Walters, Saranya Sridhar, Stefan Uderhardt, Ahmed M Salman, Benedict R Halbroth, Duncan Bellamy, Georgina Bowyer, Jonathan Powlson, Megan Baker, Navin Venkatraman, Ian Poulton, Eleanor Berrie, Rachel Roberts, Alison M Lawrie, Brian Angus, Shahid M Khan, Chris J Janse, Katie J Ewer, Ronald N Germain, Alexandra J Spencer, Adrian V S Hill
Despite recent advances in treatment and vector control, malaria is still a leading cause of death, emphasizing the need for an effective vaccine. The malaria life cycle can be subdivided into three stages: the invasion and growth within liver hepatocytes (pre-erythrocytic stage), the blood stage (erythrocytic stage), and, finally, the sexual stage (occurring within the mosquito vector). Antigen (Ag)-specific CD8+ T cells are effectively induced by heterologous prime-boost viral vector immunization and known to correlate with liver-stage protection...
September 26, 2018: Science Translational Medicine
Xin-Xin Shen, Fang-Zhou Qiu, Gui-Xia Li, Meng-Chuan Zhao, Ji Wang, Chen Chen, Li Zhao, Ju-Ju Qi, Hong Liu, Yi Zhang, Xiao-Zhou He, Le Wang, Zhi-Shan Feng, Xue-Jun Ma
Some serotypes of enterovirus (EV) may lead to transient and symptomatic gastrointestinal infections while others are commensal residents of the human gut. To determine whether certain EV types are more often associated with diarrhea, we conducted a preliminary study on the prevalence of EV serotypes and common diarrhea viruses in fecal samples of diarrhea children and healthy controls. EV was tested with one step nest polymerase chain reaction and typed by direct sequencing while common causative diarrhea viruses rotavirus (RV), norovirus (NoV), adenovirus (AdV), bocavirus (HBoV), and astrovirus (AstV) were screened with multiplex PCR assays...
September 25, 2018: Archives of Virology
Ashrafali M Ismail, Ji Sun Lee, Jeong Yoon Lee, Gurdeep Singh, David W Dyer, Donald Seto, James Chodosh, Jaya Rajaiya
Human adenovirus (HAdV) infections cause disease world-wide. Whole genome sequencing has now distinguished 90 distinct genotypes in 7 species (A-G). Over half of these 90 HAdVs fall within species D, with essentially all of the HAdV-D whole genome sequences generated in the last decade. Herein, we describe recent new findings made possible by mining of this expanded genome database, and propose future directions to elucidate new functional elements and new functions for previously known viral components.
2018: Frontiers in Microbiology
Alberto Valdés, Hongxing Zhao, Ulf Pettersson, Sara Bergström Lind
Viral infections cause large problems in the world and deeper understanding of the disease mechanisms is needed. Here we present an analytical strategy to investigate the host cell protein changes during human adenovirus type 2 (HAdV-C2 or Ad2) infection of lung fibroblasts by stable isotope labelling of amino acids in cell culture (SILAC) and nanoLC-MS/MS. This work focuses on early phase of infection (6 and 12 h post-infection (hpi)) but the data is combined with previously published late phase (24 and 36 hpi) proteomics data to produce a time series covering the complete infection...
2018: PloS One
Flávia Andressa Pidone Ribeiro, Camila Pontes, Alexandre De M V Machado, Oscar Bruna-Romero, Hananiah T Quintana, Flávia De Oliveira, José Ronnie Carvalho De Vasconcelos, Daniel Araki Ribeiro
The aim of this study was to evaluate the efficacy of vaccine using replication-deficient human recombinant Type 5 replication-defective adenoviruses (AdHu5) carrying sequences of the amastigote surface protein 2 (ASP2) (AdASP2) in mice infected with the Trypanosoma cruzi ( T cruzi) Y strain. A total of 16 A/Sn mice female were distributed into four groups, as follows (n = 4 per group): Group 1 - Control Group (CTRL); Group 2 - Infected Group (TC): animals were infected by subcutaneous route with 150 bloodstream trypomastigotes of T cruzi Y strain; Group 3 - Immunized Group (AdASP-2): animals were immunized by intramuscular injection (im) route with 50 µL of AdSP-2 (2 × 10 8 plaque forming units [pfu]/cam) at day 0; Group 4-Immunized and Infected Group (AdASP-2+TC): animals were immunized by im route with 50 µL of ASP-2 (2 × 10 8  pfu/cam) and infected by T cruzi at the same day (day 0)...
September 23, 2018: Journal of Cellular Biochemistry
Nicholas Svitek, Rosemary Saya, Elias Awino, Stephen Munyao, Robert Muriuki, Thomas Njoroge, Roger Pellé, Nicholas Ndiwa, Jane Poole, Sarah Gilbert, Vishvanath Nene, Lucilla Steinaa
The parasite Theileria parva is the causative agent of East Coast fever (ECF), one of the most serious cattle diseases in sub-Saharan Africa, and directly impacts smallholder farmers' livelihoods. There is an efficient live-parasite vaccine, but issues with transmission of vaccine strains, need of a cold chain, and antibiotics limit its utilization. This has fostered research towards subunit vaccination. Cytotoxic T lymphocytes (CTL) are crucial in combating the infection by lysing T. parva -infected cells...
2018: NPJ Vaccines
Xinwei Wu, Xiaoyan Lu, Eileen Schneider, Jamal A Ahmed, M Kariuki Njenga, Robert F Breiman, Rachel B Eidex, Dean D Erdman
Human adenoviruses (HAdVs) were previously detected at high prevalence by real-time RT-PCR (rRT-PCR) in the upper respiratory tract of residents of two Kenyan refugee camps under surveillance for acute respiratory illness (ARI) between October 2006 and April 2008. We sought to confirm this finding and characterize the HAdVs detected. Of 2148 respiratory specimens originally tested, 511 (23.8%) screened positive for HAdV and 510 were available for retesting. Of these, 421 (82.4%) were confirmed positive by repeat rRT-PCR or PCR and sequencing...
September 22, 2018: Journal of Medical Virology
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