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human adenovirus infection

R De, L Y Liu, Y Qian, L P Jia, H J Dong, R N Zhu, Y H Liu, Y Q Lyu, L Q Zhao
Objective: To investigate the relationship between human bocavirus 2 (HBoV2) infection and acute diarrhea in children younger than 5 years of age in a case-control study. Methods: This was a prospective case-control study. During May 2016 to December 2016, fecal specimens were collected from children ≤5 years of age with acute diarrhea who visited the Affiliated Children's Hospital of Capital Institute of Pediatrics (case group), or from children ≤5 years of age without diarrhea from Longtan Community Medical Service Center, Beijing (control group)...
December 2, 2018: Zhonghua Er Ke za Zhi. Chinese Journal of Pediatrics
Busen Wang, Jianhua Li, Shipo Wu, Yi Chen, Zhe Zhang, Yanfang Zhai, Qiang Guo, Jinlong Zhang, Xiaohong Song, Zhenghao Zhao, Lihua Hou, Wei Chen
An apparent increase in the frequency of human adenovirus type 4 (HAdV-4) infections among general populations has been observed over the past 10 years. However, available epidemiological data that may reflect previous viral circulation and assist in predicting potential outbreaks are sparse, particularly in mainland China and Africa. In this study, a convenient neutralization assay for use in the surveillance of historical HAdV-4 infections was established based on a recombinant luciferase-expressing virus...
December 5, 2018: Emerging Microbes & Infections
Seyyed Mehdy Elahi, Chun Fang Shen, Rénald Gilbert
During the last decade, oncolytic viruses such as vesicular stomatitis virus (VSV) have gained tremendous popularity as efficient vaccines for infectious diseases as well as for the treatment of cancer. Our laboratory has developed two stable cell lines, 293SF-3F6 (derived from HEK293 A cells) and SF-BMAdR cells (a variant of A549 that expresses the E1 region of human adenovirus). These two cell lines were adapted to grow efficiently in suspension culture and in serum-free medium. In this report we evaluated the production of a recombinant VSV expressing the green fluorescent protein (VSV-GFP) in these two stable cell lines...
November 30, 2018: Journal of Biotechnology
Arthur Dyer, Benjamin Schoeps, Sally Frost, Philip G Jakeman, Eleanor M Scott, Joshua Freedman, Leonard W Seymour
Tumour cells exhibiting the Warburg effect rely on aerobic glycolysis for ATP production and have a notable addiction to anaplerotic use of glutamine for macromolecular synthesis. This strategy maximises cellular biosynthetic potential while avoiding excessive depletion of NAD+, and provides an attractive anabolic environment for viral infection. Here we evaluate infection of highly permissive and poorly permissive cancer cells with wild type adenoviruses and the oncolytic chimeric adenovirus enadenotucirev (EnAd)...
November 28, 2018: Cancer Research
Isabelle Chareyron, Christopher Wall, Jonathan Thevenet, Jaime Santo-Domingo, Andreas Wiederkehr
Changes in mitochondrial and cytosolic pH alter the chemical gradient across the inner mitochondrial membrane. The proton chemical gradient contributes to mitochondrial ATP synthesis as well as the uptake and release of metabolites and ions from the organelle. Here mitochondrial pH and ΔpH were studied for the first time in human pancreatic β-cells. Adenoviruses were used for rat insulin promoter dependent expression of the pH sensor SypHer targeted to either the mitochondrial matrix or the cytosol. The matrix pH in resting human β-cells is low (pH = 7...
November 23, 2018: Molecular and Cellular Endocrinology
K-A Hwang, S Park, J H Ahn, J-H Nam
It has been previously reported that adenovirus 36 (Ad36) infection is associated with obesity in humans and other animals. However, there is no clinically available standard protocol to detect Ad36 DNA. In this study, we developed a method for quantitative and rapid detection of Ad36 DNA. Using a TaqMan probe quantitative polymerase chain reaction (qPCR), we identified that the E3 and E4orf1 regions specifically detect Ad36 DNA, because these regions did not show cross reactivity with other types of adenoviruses...
2018: Acta Virologica
Ali Zhang, Tanner M Tessier, Kristianne J C Galpin, Cason R King, Steven F Gameiro, Wyatt W Anderson, Ahmed F Yousef, Wen T Qin, Shawn S C Li, Joe S Mymryk
Early region 1A (E1A) is the first viral protein produced upon human adenovirus (HAdV) infection. This multifunctional protein transcriptionally activates other HAdV early genes and reprograms gene expression in host cells to support productive infection. E1A functions by interacting with key cellular regulatory proteins through short linear motifs (SLiMs). In this study, the molecular determinants of interaction between E1A and BS69, a cellular repressor that negatively regulates E1A transactivation, were systematically defined by mutagenesis experiments...
November 22, 2018: Viruses
Walter Doerfler, Stefanie Weber, Anja Naumann
Apart from its well-documented role in long-term promoter silencing, the genome-wide distribution patterns of ~28 million methylated or unmethylated CpG dinucleotides, e. g. in the human genome, is in search of genetic functions. We have set out to study changes in the cellular CpG methylation profile upon introducing foreign DNA into mammalian cells. As stress factors served the genomic integration of foreign (viral or bacterial plasmid) DNA, virus infections or the immortalization of cells with Epstein Barr Virus (EBV)...
November 21, 2018: Epigenetics: Official Journal of the DNA Methylation Society
Kwangchol Mun, Tanel Punga
Human adenovirus (HAdV) encodes a multifunctional DNA-binding protein pVII, which is involved in virus DNA packaging and extracellular immune signaling regulation. Although the pVII is an essential viral protein, its exact role in the virus life cycle and interplay with cellular proteins have remained to a large extent unclear. We have recently identified the cellular zinc finger protein 622 (ZNF622) as a potential pVII-interacting protein. In this study, we describe the functional consequences of the ZNF622-pVII interplay and the role of ZNF622 in the HAdV life cycle...
November 14, 2018: Journal of Virology
Laetitia Kaas, Leslie Ogorzaly, Gaël Lecellier, Véronique Berteaux-Lecellier, Henry-Michel Cauchie, Jérémie Langlet
Lack of wastewater treatment efficiency causes receiving seawaters and bivalve molluscan shellfish to become contaminated, which can lead to public health issues. Six wastewater samples, five seawater samples and three batches of giant clams from Tahiti (French Polynesia) were investigated for the presence of enteric viruses, but also if present, for the diversity, infectivity and integrity of human adenoviruses (HAdV). Enteroviruses (EV), sapoviruses (SaV) and human polyomaviruses (HPyV) were detected in all wastewater samples...
November 13, 2018: Food and Environmental Virology
Parvaiz A Koul, Hyder Mir, Siddhartha Saha, Mandeep S Chadha, Varsha Potdar, Marc-Alain Widdowson, Renu B Lal, Anand Krishnan
Background & objectives: Respiratory tract infections are common among Hajj and Umrah pilgrims which pose a public health risk of spread of respiratory infections. Influenza has been reported from Indian Hajj and Umrah returning pilgrims, but data on other respiratory pathogens are sparse in India. Here we report the presence of common respiratory viral pathogens in returning Hajj and Umrah pilgrims suffering from acute respiratory illness (ARI) in 2014-2015. Methods: Respiratory specimens (nasopharyngeal and throat swabs) were collected from 300 consenting pilgrims with ARI in the past one week and tested for influenza and Middle East Respiratory Syndrome coronavirus (MERS-CoV) and other respiratory viruses using in-house standardized quantitative real-time reverse-transcription polymerase chain reaction...
September 2018: Indian Journal of Medical Research
Hongdou Jia, Xiaobing Li, Guowen Liu, Juan J Loor, Ryan Buctrout, Xudong Sun, Guojin Li, Xin Shu, Jihong Dong, Yazhe Wang, Rankun Zuo, Zhe Wang, Xinwei Li
Fatty liver is a common metabolic disorder in dairy cows during the transition period. Perilipin 5 (PLIN5), a lipid droplet coat protein, plays important roles in the development of hepatic steatosis in mice and humans. Whether PLIN5 plays a role in the development of fatty liver in dairy cows is unknown. An in vivo study consisting of 10 healthy and 10 cows with fatty liver was performed to harvest liver tissue and blood samples. In addition, hepatocytes isolated from calves were infected with PLIN5 overexpression adenovirus for 48 h; treated with 0, 0...
November 8, 2018: Journal of Dairy Science
Feng Huang, Junsong Zhang, Diyuan Yang, Yuelan Zhang, Jinxiang Huang, Yaochang Yuan, Xuefeng Li, Gen Lu
Human adenovirus (Adv) infection is responsible for most community-acquired pneumonia in infants and children, which results in significant morbidity and mortality in children every year. MicroRNAs (miRNAs) are associated with viral replication and host immune response. Knowing the miRNA expression profile will help understand the role of miRNAs in modulating the host response to adenovirus infection and possibly improve the diagnosis of adenovirus-infected pneumonia. In our study, total RNA extracted from whole blood of adenovirus-infected pneumonia children and healthy controls were analyzed by small RNA deep sequencing...
2018: Mediators of Inflammation
Fangfang Li, Yange Lang, Zhenglin Ji, Zhiqiang Xia, Yuewen Han, Yuting Cheng, Gaomin Liu, Fang Sun, Yonghui Zhao, Minjun Gao, Zongyun Chen, Yingliang Wu, Wenxin Li, Zhijian Cao
Viral infection still threatens human health all over the world, and many people die from viral diseases every year. However, there are no effective vaccines or drugs for preventing or managing most viral diseases. Thus, the discovery and development of broad-spectrum antiviral agents remain urgent. Here, we expressed and purified a scorpion venom peptide, Ev37, from the scorpion Euscorpiops validus in a prokaryotic system. We found that rEv37 can inhibit dengue virus type 2 (DENV-2), hepatitis C virus (HCV), Zika virus (ZIKV) and herpes simplex virus type 1 (HSV-1) infections in a dose-dependent manner at noncytotoxic concentrations, but that it has no effect on Sendai virus (SeV) and Adenovirus (AdV) infections in vitro...
November 7, 2018: Journal of Biological Chemistry
Liubov Biliavska, Yuliia Pankivska, Olga Povnitsa, Svitlana Zagorodnya, Ganna Gudz, Yuriy Shermolovich
Background and objectives: A considerable increase in the levels of adenoviral diseases among both adults and children necessitate the development of effective methods for its prevention and treatment. The synthesis of the new fluorinated 1,2,3-triazoles, and the study of the mechanisms of their action, are promising for the development of efficient antiviral drugs of our time. Materials and Methods: Antiviral activity and cell cytotoxic effect of 2-(3-chlorotetrahydrofuran-2-yl)-4-tosyl-5-(perfluoropropyl)-1,2,3-triazole (G29) were determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assay...
November 5, 2018: Medicina
Dieli Primo, Gabriela Tayna Pacheco, Maria do Carmo Sampaio Tavares Timenetsky, Adriana Luchs
BACKGROUND: In Brazil, limited data are available regarding the incidence and molecular epidemiology of Human Adenovirus (HAdV) in diarrheic disease, especially in the post rotavirus (RVA) vaccine era. OBJECTIVE: The aims of the study were to investigate the frequency of HAdV infections in patients with gastroenteritis during a 6-year period (2012-2017); conduct molecular typing of positive strains, and obtain further information on the HAdV seasonality. STUDY DESIGN: A total of 3003 fecal samples negative for both, RVA and Norovirus, were selected and tested for HAdV by PCR...
November 1, 2018: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
Kattareeya Kumthip, Pattara Khamrin, Aphisek Kongkaew, Ratchaya Vachirachewin, Rungnapa Malasao, Hiroshi Ushijima, Niwat Maneekarn
Pigs have been assumed as a source of human viral infections. Surveillance of viruses in animals is essential to evaluate the risk to human and animal health and to determine economic impact. A number of studies focused mainly on well- known enteritis viruses such as porcine epidemic diarrhea virus (PEDV), transmissible gastroenteritis virus (TGEV), and porcine group A rotavirus (PRVA), however, little data is available for porcine adenovirus (PAdV). In this study, the presence of PAdV was investigated in fecal samples collected from piglets with and without diarrhea from 31 commercial pig farms in northern Thailand...
November 2, 2018: Infection, Genetics and Evolution
Luciana Nascimento Pinto Canela, Maria Clara de Magalhães-Barbosa, Carlos Eduardo Raymundo, Sharon Carney, Marilda Mendonca Siqueira, Arnaldo Prata-Barbosa, Antonio José Ledo Alves da Cunha
OBJECTIVES: The role of viral co-detection in children with severe acute respiratory infection is not clear. We described the viral detection profile and its association with clinical characteristics in children admitted to the Pediatric Intensive Care Unit (PICU) during the 2009 influenza A(H1N1) pandemic. METHOD: Longitudinal observational retrospective study, with patients aged 0-18 years, admitted to 11 PICUs in Rio de Janeiro, with suspected H1N1 infection, from June to November, 2009...
October 23, 2018: Brazilian Journal of Infectious Diseases
Oskar Bunz, Kemal Mese, Wenli Zhang, Andree Piwowarczyk, Anja Ehrhardt
More than 70 human adenovirus types were identified divided into 7 different species (A-G). Diseases caused by human adenoviruses are type-dependent and can range from mild to severe respiratory infections, gastrointestinal infections or eye infections such as epidemic keratoconjunctivitis. Unfortunately there is no specific anti-adenovirus therapy available. Here we addressed the question whether treatment with cold atmospheric plasma (CAP) for anti-adenoviral therapy such as virus-mediated ulcerations may be feasible...
2018: PloS One
Marco Verani, Ileana Federigi, Gabriele Donzelli, Lorenzo Cioni, Annalaura Carducci
The current microbial water quality standards are based on the monitoring of fecal indicator organisms, which are mainly bacterial indicators (i.e., Escherichia coli, intestinal enterococci), however epidemiological data indicate that viruses are important etiological agents of waterborne illnesses. Among waterborne viruses, human adenovirus can be considered as an index pathogen, owing to its abundance in sewage and persistence in the environment, as well as its potential infectivity. In this study, data on human adenoviruses from different water matrices (the entrance and exit of a water treatment plant, rivers and seawaters) were analyzed, in parallel with traditional fecal bacterial indicators and somatic coliphages...
February 15, 2019: Science of the Total Environment
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