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high mobility group box-1 protein 1

Zhifei Xu, Ying Jin, Hao Yan, Zizheng Gao, Bo Xu, Bo Yang, Qiaojun He, Qiang Shi, Peihua Luo
Dasatinib shows remarkable activity against imatinib-refractory chronic myelogenous leukemia (CML) and Philadelphia chromosome positive acute lymphoblastic leukemia (Ph+ ALL). However, severe cardiovascular toxicity limits the clinical applications of dasatinib. Since the underlying mechanism of dasatinib-induced cardiotoxicity is still elusive, we aim to clarify this. Recent studies have shown that necroptosis and apoptosis participate in multiple toxicity development. Here, we first report that dasatinib could directly induce cardiomyocytes death, as analyzed by the Sulforhodamine B (SRB) assay...
August 4, 2018: Toxicology Letters
Wei Zhang, Jian Song, Yue Zhang, Yingxue Ma, Jing Yang, Guanghui He, Song Chen
BACKGROUND: In this study, we evaluated the effects of intermittent high glucose on oxidative stress production in retinal pigmented epithelial (RPE) cells and explored whether the mechanisms of autophagy and apoptosis in oxidative stress are associated with high-mobility group box 1 (HMGB1) protein. METHODS: Cultured human RPE cell line ARPE-19 cells were exposed to intermittent high glucose-induced oxidative stress. Reactive oxygen species (ROS) was determined by 2', 7'-dichlorofluorescin diacetate (DCFH-DA); and malonyldialdehyde (MDA), superoxide dismutase (SOD) by commercial kits...
August 6, 2018: BMC Ophthalmology
Ewa Pasquereau-Kotula, Justine Habault, Guido Kroemer, Jean-Luc Poyet
In recent years, immunogenic cell death (ICD) has emerged as a revolutionary concept in the development of novel anticancer therapies. This particular form of cell death is able, through the spatiotemporally defined emission of danger signals by the dying cell, to induce an effective antitumor immune response, allowing the immune system to recognize and eradicate malignant cells. To date, only a restricted number of chemotherapeutics can trigger ICD of cancer cells. We previously reported that a peptide, called RT53, spanning the heptad leucine repeat region of the survival protein AAC-11 fused to a penetrating sequence, selectively induces cancer cell death in vitro and in vivo...
2018: PloS One
Kunlin Wu, Huihao Zhang, Yajuan Fu, Youzhi Zhu, Lingjun Kong, Ling Chen, Feng Zhao, Liangfei Yu, Xiangjin Chen
The influence of Toll‑like receptor (TLR)4/myeloid differentiation factor (MyD)88 signaling on the invasion and metastasis of cancer cells has been previously reported. The purpose of the present study was to determine the role of TLR4/MyD88 in breast cancer cell migration and invasion, and to discover novel therapeutic targets for breast cancer treatment. TLR4, MyD88 and high mobility group box 1 (HMGB1) mRNA expression levels were assessed in highly invasive human MDA‑MB‑231 breast cancer cells, breast cancer cells with a low rate of invasion (MCF‑7) and normal human MDA‑Kb2 mammary gland cells by reverse transcription‑quantitative polymerase chain reaction...
July 26, 2018: Molecular Medicine Reports
Maoliang Huang, Yan Geng, Qiaoting Deng, Ru Li, Xiangyang Shao, Zhigao Zhang, Weiwen Xu, Yingsong Wu, Qiang Ma
Recently, accumulating evidence from clinical and experimental researches have suggested that translationally controlled tumor protein (TCTP) and high mobility group box 1 (HMGB1) are implicated in colorectal cancer (CRC) metastasis. However, whether there is an interconnection between these two tumor-promoting proteins and how they affect CRC metastasis remain to be fully elucidated. In the present study, the expression level of TCTP in CRC tissues was assessed by immunohistochemical staining and immunoblotting, and the serum concentration of HMGB1 in patients with CRC was detected by enzyme-linked immunosorbent assay...
July 25, 2018: International Journal of Oncology
Jun Xu, Jin-Woo Lee, Soo-Kyoung Park, Sung-Bok Lee, Young-Hoon Yoon, Sun-Hee Yeon, Ki-Sang Rha, Ji-Ae Choi, Chang-Hwa Song, Yong Min Kim
B-cell activating factor (BAFF) has been proposed to play a crucial role in the pathogenesis of chronic rhinosinusitis with nasal polyp (CRSwNP). The aim of this study was to evaluate the role of toll-like receptor (TLR) 9-mediated BAFF activation on the pathogenesis of CRSwNP. NP and uncinate tissue (UT) were obtained from patients with CRSwNP or CRS without NP, and control subjects. The expression of TLR9, high mobility group box-1 protein (HMGB1), type I interferon (IFN), BAFF, and anti-double stranded DNA (dsDNA) antibody were examined in the tissues and the cultured dispersed NP cells (DNPCs)...
July 26, 2018: Clinical Immunology: the Official Journal of the Clinical Immunology Society
Xue Zhang, Guanghua Zhang, Yan Ren, Tianchi Lan, Defang Li, Jingwei Tian, Wanglin Jiang
Irinotecan (CPT-11) is used to treat various cancers but side effects such as delayed diarrhea restrict its use. Darunavir (DRV) is an antiretroviral drug used to treat and prevent human immunodeficiency virus/acquired immune deficiency syndrome (HIV/AIDS), but whether DRV is protective against CPT-11-induced intestinal toxicity is unclear. An CPT-11-induced intestinal toxicity model was produced using uninterrupted CPT-11 (ip) for 4 d in mice. Enzyme-linked immuno sorbent assay (ELISA), fecal occult blood test (FOBT), Western blot, histopathological evaluation, and immunohistochemistry staining assays were used to document toxicity...
July 24, 2018: European Journal of Pharmacology
Huawei Gu, Ji Liu, Shuyi Chen, Haonan Qi, Kan Shi, Shan Li, Yi Ma, Jufang Wang
Clostridium difficile TcdB is a key virulence factor that causes C. difficile-associated diseases. Our previous studies have shown that recombinant full-length TcdB (rTcdB) induces cell death in CT26 cells, and rTcdB-treated CT26 cells with high immunogenicity could stimulate dendritic cell (DC) activation and T cell activation in vitro. The rTcdB-treated CT26 cells also induce antitumor immunity in mice and protect mice from CT26 cells. High-mobility group box 1 protein (HMGB1) is a non-histone nuclear protein, which has various biological functions within the nucleus and also acts as an extracellular signal molecule involving in inflammatory diseases, cancers or autoimmune diseases...
July 20, 2018: Acta Biochimica et Biophysica Sinica
Xiangsheng Zhang, Yue Lu, Qi Wu, Haibin Dai, Wei Li, Shengyin Lv, Xiaoming Zhou, Xin Zhang, Chunhua Hang, Jian Wang
Inflammation plays a key role in the progression of subarachnoid hemorrhage (SAH). Here, we examined the effects of astaxanthin (ATX) on the inflammatory response and secondary damage after SAH and the underlying mechanisms of action. In vivo, a prechiasmatic cistern injection model was established in rats and mice. In addition, neuron-microglia cocultures were exposed to oxyhemoglobin to mimic SAH in vitro. Western blotting revealed that protein expression of TLR4 was markedly increased in microglia at 24 h after SAH, with consequent increases in the downstream molecules myeloid differentiation factor 88 and NF-кB...
July 26, 2018: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
Eusebio Chiefari, Daniela P Foti, Riccardo Sgarra, Silvia Pegoraro, Biagio Arcidiacono, Francesco S Brunetti, Manfredi Greco, Guidalberto Manfioletti, Antonio Brunetti
HMGA1 (high mobility group A1) is a nonhistone architectural chromosomal protein that functions mainly as a dynamic regulator of chromatin structure and gene transcription. As such, HMGA1 is involved in a variety of fundamental cellular processes, including gene expression, epigenetic regulation, cell differentiation and proliferation, as well as DNA repair. In the last years, many reports have demonstrated a role of HMGA1 in the transcriptional regulation of several genes implicated in glucose homeostasis...
2018: Frontiers in Endocrinology
Qian Li, Zhuo-Ming Li, Shu-Ya Sun, Lu-Ping Wang, Pan-Xia Wang, Zhen Guo, Han-Wei Yang, Jian-Tao Ye, Jing Lu, Pei-Qing Liu
High-mobility group box 1 (HMGB1) exhibits various functions according to its subcellular location, which is finely conditioned by diverse post-translational modifications, such as acetylation. The nuclear HMGB1 may prevent from cardiac hypertrophy, whereas its exogenous protein is proven to induce hypertrophic response. This present study sought to investigate the regulatory relationships between poly(ADP-ribose) polymerase 1 (PARP1) and HMGB1 in the process of pathological myocardial hypertrophy. Primary-cultured neonatal rat cardiomyocytes (NRCMs) were respectively incubated with three cardiac hypertrophic stimulants, including angiotensin II (Ang II), phenylephrine (PE), and isoproterenol (ISO), and cell surface area and the mRNA expression of hypertrophic biomarkers were measured...
July 20, 2018: Acta Pharmacologica Sinica
Vengadeshprabhu Karuppagounder, Anamika Bajpai, Shu Meng, Somasundaram Arumugam, Remya Sreedhar, Vijayasree V Giridharan, Ashrith Guha, Arvind Bhimaraj, Keith A Youker, Suresh S Palaniyandi, Harry Karmouty-Quintana, Fadia Kamal, Kara L Spiller, Kenichi Watanabe, Rajarajan A Thandavarayan
The purpose of this study was to determine whether blocking of G protein βγ (Gβγ) signaling halts heart failure (HF) progression by macrophage phenotype manipulation. Cardiac Gβγ signaling plays a crucial role in HF pathogenesis. Previous data suggested that inhibiting Gβγ signaling reprograms T helper cell 1 (Th1) and Th2 cytokines, suggesting that Gβγ might be a useful drug target for treating HF. We investigated the efficacy of a small molecule Gβγ inhibitor, gallein, in a clinically relevant, experimental autoimmune myocarditis (EAM) model of HF as well as in human macrophage phenotypes in vitro...
2018: PloS One
Yan Zou, Wenting Dai, Wenbo Lei, Shengmei Su, Qiulin Huang, Zhou Zhou, Chaoqun Chen, Zhongyu Li
OBJECTIVE: This study is to identify and investigate the proteins interacting with pORF5 implicated in the pathogenesis of C. trachomatis . METHODS: The isobaric tags for relative and absolute quantitation (iTRAQ) approach combined with nano liquid chromatography-tandem mass spectrometry (NanoLC-MS/MS) analysis was applied to identify and quantify the differentially expressed proteins in the pORF5-transfected HeLa (pORF5-HeLa) cells and the control vector-transfected HeLa (vector-HeLa) cells...
2018: American Journal of Translational Research
Zilin Cui, Shipeng Li, Zirong Liu, Yamin Zhang, Haiming Zhang
BACKGROUND/AIMS: Interferon regulatory factor 1(IRF-1) and high mobility group box 1(HMGB1) have been independently identified as being key players in hepatic ischemia-reperfusion injury (IRI). We attempted to determine whether IRF-1 activates autophagy to aggravate hepatic IRI by increasing HMGB1 release. METHODS: The hepatic IRI model was generated in C57BL/6 mice, euthanized at 2, 6, 12 or 24 h after reperfusion. To examine the effects of HMGB1 release inhibition, Glycyrrhiza acid (GA) was administered to the mice and at six hours after injectiont...
July 17, 2018: Cellular Physiology and Biochemistry
Yuanyuan Wang, Changli Wang, Dan Zhang, Huihui Wang, Lulong Bo, Xiaoming Deng
BACKGROUND Traumatic brain injury (TBI) leads to acute lung injury (ALI), in which the inflammatory response plays an important role in its pathophysiology. Recent studies suggest that dexmedetomidine (Dex) plays a protective role in acute inflammatory diseases. However, whether Dex has a protective effect on TBI-induced ALI is not clear. The aim of this study was to investigate the effect of Dex on TBI-induced ALI in mice. MATERIAL AND METHODS Mice were randomly divided into 5 groups: 1) sham group; 2) TBI group; 3) TBI+Dex group; 4) TBI+atipamezole (Atip) group; and 5) TBI+Dex+Atip group...
July 17, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
Philipp Houben, Ralph Hohenberger, Kenya Yamanaka, Markus W Büchler, Peter Schemmer
BACKGROUND Pre-transplant assessment of the graft for liver transplantation is crucial. Based on experimental data, this study was designed to assess both nuclear high mobility group box-1 (HMGB-1) protein and arginine-specific proteolytic activity (ASPA) in the graft effluent. MATERIAL AND METHODS In a non-interventional trial, both HMGB-1 and ASPA were measured in the effluent of 30 liver grafts after cold storage before transplantation. Values of HMGB-1 and ASPA levels were compared with established prognostic parameters such as the donor risk index, balance of risk score, and Donor-Model for End-Stage Liver Disease...
July 13, 2018: Annals of Transplantation: Quarterly of the Polish Transplantation Society
Esther Bandala-Sanchez, Naiara G Bediaga, Ethan D Goddard-Borger, Katrina Ngui, Gaetano Naselli, Natalie L Stone, Alana M Neale, Lesley A Pearce, Ahmad Wardak, Peter Czabotar, Thomas Haselhorst, Andrea Maggioni, Lauren A Hartley-Tassell, Timothy E Adams, Leonard C Harrison
CD52, a glycophosphatidylinositol (GPI)-anchored glycoprotein, is released in a soluble form following T cell activation and binds to the Siglec (sialic acid-binding Ig-like lectin)-10 receptor on T cells to suppress their function. We show that binding of CD52-Fc to Siglec-10 and T cell suppression requires the damage-associated molecular pattern (DAMP) protein, high-mobility group box 1 (HMGB1). CD52-Fc bound specifically to the proinflammatory Box B domain of HMGB1, and this in turn promoted binding of the CD52 N-linked glycan, in α-2,3 sialic acid linkage with galactose, to Siglec-10...
July 24, 2018: Proceedings of the National Academy of Sciences of the United States of America
L Wei, Z J Han, L Xu, J W Li
Objective: To establish the ischemia reperfusion injury model in rat after lung transplantation(LT) and explore the expression of high mobility group box 1 protein(HMGB1) after intravenous injection with bone marrow mesenchymal stem cells(MSCs). Methods: Forty healthy 8-10 weeks male SD rats were randomly divided into four groups including the sham-operated group, ischemia-reperfusion (IR), Saline-IR and MSC-IR group. The sham-operated rats were only conducted thoracotomy without lung transplantation and the rest groups were respectively conducted with the left LT, left LT followed by 1 ml saline and left LT followed by 1 ml MSCs (1...
July 3, 2018: Zhonghua Yi Xue za Zhi [Chinese medical journal]
Karen Ingrid Tasca, Camila Renata Correa, Juliana Trindade Caleffi, Monica Banwart Mendes, Mariana Gatto, Vanessa Martinez Manfio, Caio Cavassan de Camargo, Francilene Capel Tavares, Mara Biasin, Lenice do Rosário de Souza
We aimed to analyze markers of immune activation, inflammation, and oxidative stress in 92 asymptomatic HIV-infected patients according to the adequate (AR, >500 cells/mm3 ) or inadequate (IR, <500 cells/mm3 ) CD4+ T recovery and the presence or absence of antiretroviral treatment (cART). In relation to those newly diagnosed, they were divided into two groups, cART-naïve IR (nIR) and cART-naïve AR (nAR). Among those diagnosed more than five years ago, the following division was made: the cART-naïve long-term nonprogressors (LTNP); patient under cART and AR (tAR); and patients under cART and IR (tIR)...
2018: Journal of Immunology Research
Gowri Palanissami, Solomon F D Paul
Risk of cancer especially of colon, breast, and pancreas is high in diabetic and obese patients, with potential involvement of augmented expression of RAGE (receptor for advanced glycation end products) and its ligands, namely AGEs (advanced glycation end products), HMGB1 (high-mobility group box 1 protein), and S100 group of proteins. Studies have reported the involvement of RAGE activation by its ligands in growth and survival of cancers, including metastasis and poor prognosis. We propose that this receptor-ligand axis provides the molecular link between certain pre-existing states as hypoxia, hyperglycemia, glycation, inflammation, oxidative stress, and onset of cancers...
July 9, 2018: Hormones & Cancer
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