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high mobility group box-1 protein 1

Shinkichi Takamori, Fumihiro Shoji, Tatsuro Okamoto, Yuka Kozuma, Taichi Matsubara, Naoki Haratake, Takaki Akamine, Masakazu Katsura, Kazuki Takada, Gouji Toyokawa, Tetsuzo Tagawa, Yoshihiko Maehara
BACKGROUND: Although high-mobility group box-1 (HMGB1), which is a nuclear protein, was reported to enhance the allogeneic responses in transplantation, the effect of HMGB1 on bronchiolitis obliterans syndrome (BOS) is unknown. METHODS: A murine heterotopic tracheal transplantation model was used. Protein concentrations of HMGB1, interferon-γ (IFN-γ), interleukin (IL)-10, and IL-17 were analyzed in the isografts, allografts, controls, and HMGB1-neutralizing antibody administered allografts (n = 6; Days 1, 3, 5, 7, 14, 21, and 28)...
November 30, 2018: Transplant Immunology
Yin Chaoyang, Bai Qingfeng, Feng Jinxing
Asthma is a complex, chronic inflammatory disorder of the bronchial tree, and can affect patients of all ages including children. High mobility group box 1 (HMGB1) has been proved as a therapeutic target in children with asthma, and was predicted to be the target gene of microRNA-216a-5p (miR-216a-5p). The present study aimed to investigate the function of miR-216a-5p in asthma by creating a human bronchial epithelial cell (16HBE) injury model using H₂O₂. A significantly elevation of HMGB1 protein expression and a reduction of miR-216a-5p expression were observed in children with asthma as well as in H₂O₂ stimulated 16HBE cells...
November 27, 2018: Biochemical and Biophysical Research Communications
Line H Tangerås, Gabriela B Silva, Guro S Stødle, Lobke M Gierman, Bente Skei, Karin Collett, Anne-Lise Beversmark, Ragnhild B Skråstad, Liv Cecilie V Thomsen, Line Bjørge, Ann-Charlotte Iversen
INTRODUCTION: Normal pregnancy is characterized by an elevated inflammatory state involving the placenta. The placental inflammation is further increased in preeclampsia, resulting in release of harmful danger signals to the maternal circulation. Activation of toll-like receptors (TLR)2 and TLR4 by endogenous danger signals plays a role in inflammatory diseases. Placental TLR2 and TLR4 expression has been reported, and high mobility group box 1 (HMGB1) is a likely endogenous activator of these receptors...
December 2018: Placenta
Kyria M Webster, Mujun Sun, Peter J Crack, Terence J O'Brien, Sandy R Shultz, Bridgette D Semple
Accumulating research suggests that children may be more vulnerable to poor long-term outcomes after traumatic brain injury (TBI) compared to adults. The neuroinflammatory response, known to contribute to neuropathology after TBI, appears to differ depending upon age-at-insult, though this response has not been well-characterized. Elevated levels of a key initiator of inflammation, high mobility group box protein 1 (HMGB1), have been associated with worsened outcomes after TBI in young patients. This study therefore aimed to characterize the acute time course of key mediators of the inflammatory cascade, including HMGB1, after pediatric and adult TBI...
November 30, 2018: Journal of Comparative Neurology
Jan-Frieder Harmsen, Alexander Franz, Constantin Mayer, Christoph Zilkens, Bettina Alexandra Buhren, Holger Schrumpf, Rüdiger Krauspe, Michael Behringer
PURPOSE: The tensiomyography (TMG) technique is increasingly used to determine muscle contractile properties in exercise and injury management. The present study investigated the informative value of TMG parameters in correlation with commonly used (creatine kinase, CK; myoglobin, Mb) and novel candidate biomarkers of muscle damage (heart-type fatty acid-binding protein, h-FABP; high-mobility group box 1, HMGB1). METHODS: Ten untrained men performed 6 × 10 eccentric contractions of the elbow flexors at 110% of the concentric one repetition maximum...
November 29, 2018: European Journal of Applied Physiology
Won Seok Yang, Jin Ju Kim, Mee Jeong Lee, Eun Kyoung Lee, Su-Kil Park
BACKGROUND/AIMS: High-mobility group box 1 (HMGB1) elicits inflammatory responses through interactions with the receptor for advanced glycation end products (RAGE) and toll-like receptor 4 (TLR4). We investigated how RAGE and TLR4 expressions are regulated after HMGB1 stimulation in cultured human aortic endothelial cells (HAECs). METHODS: RAGE and TLR4 expressions were analyzed by Western blot analysis and immunofluorescence staining. A disintegrin and metalloprotease 17 (ADAM17) activity was measured using a fluorogenic substrate...
November 29, 2018: Cellular Physiology and Biochemistry
Marie-Eve Brien, Bernadette Baker, Cyntia Duval, Virginie Gaudreault, Rebecca Lee Jones, Sylvie Girard
Inflammation is known to be associated with placental dysfunction and pregnancy complications. Infections are well known to be a cause of inflammation but they are frequently undetectable in pregnancy complications. More recently, the focus has been extended to inflammation of non-infectious origin, namely caused by endogenous mediators known as "damage-associated molecular patterns - DAMPs" or alarmins. In this manuscript we review the mechanism by which inflammation; sterile or infectious can alter the placenta and its function...
November 28, 2018: Canadian Journal of Physiology and Pharmacology
Sheng Liu, Hong-Ze Chen, Zheng-Dong Xu, Fei Wang, Haoshu Fang, Ophelia Bellanfante, Xu-Lin Chen
BACKGROUND: Inflammatory response triggered by high mobility group box-1 (HMGB1) protein and oxidative stress play critical roles in the intestinal injury after severe burn. Sodium butyrate, a histone deacetylase inhibitor, has potential anti-inflammatory properties, inhibiting the expression of inflammatory mediators such as HMGB1 in diverse diseases. This study was designed to investigate the effects of sodium butyrate on severe burn plus delayed resuscitation-induced intestine injury, intestinal expressions of HMGB1 and intracellular adhesion molecule-1 (ICAM-1), oxidative stress, and signal transduction pathway changes in rats...
October 25, 2018: Burns: Journal of the International Society for Burn Injuries
Junying Qiao, Lixia Chen, Xianjie Huang, Feifei Guo
OBJECTIVE: To investigate the role of high mobility group box 1 (HMGB1) and receptor for advanced glycation end product (RAGE) in the lungs of hyperoxia-induced rats and the effect of N--acetlycystein (NAC). METHODS: A model of hyperoxic lung injury was established, rats in the NAC intervention, and control, hyperoxia group were given nebulized NAC aerosol, nebulized same volume of saline once a day for 7 consecutive days, respectively. Wet/dry ( W/ D) ratio of the lungs was determined to evaluate the edema of the lung tissues...
November 27, 2018: Journal of Cellular Physiology
Dong Eun Kim, Albert R Davalos
Senescent cells secrete diverse array of proteins. One group of proteins, damage-associated molecular pattern (DAMP) proteins exhibit relocalization from inside to outside the cell. High Mobility Group Box 1 protein (HMGB1) is the founding DAMP member. HMGB1 relocalization from the nucleus provides a molecular signature during senescence. We provide distinct molecular techniques (immunofluorescence, immunohistochemistry, and Western blot assays) to assess HMGB1 relocalization during the initial stages of senescence...
2019: Methods in Molecular Biology
Yi Liu, Guo-Bin Zhuang, Xue-Zhi Zhou
High-mobility group box 1 (HMGB1) is a nuclear protein that can also act as an extracellular trigger of inflammation, proliferation, and migration in eye diseases. It induces signaling pathways by binding to the receptor for advanced glycation end products (RAGE) and Toll-like receptors (TLRs) 2, 4, and 9. This proinflammatory activity is considered to be important in the pathogenesis of a wide range of ocular diseases resulting from hemodynamic changes, presence of neovascular endothelial cells, secretion of intraocular immune factors or inflammation, and apoptosis of retinal cell layers...
2018: Journal of Ophthalmology
Ji Li, Yuting Han, Yue Lu, Baohui Song, Ming Zhao, Haiyang Hu, Dawei Chen
Background: Polyamidoamine (PAMAM) dendrimers modified by polyethylene glycol (PEG) have frequently been investigated as a delivery carrier for gene therapy. However, modification of PAMAM with PEG using covalent linkage significantly reduces the cellular uptake rate and the transfection efficiency. How to conquer these barriers becomes a burning question in gene delivery. Materials and methods: The present study constructed an effective disulfide bond-mediated cleavable RGD modified gene delivery system to overcome the aforementioned limitations...
2018: International Journal of Nanomedicine
Yu Wang, Xiaofeng Wang, Wenping Yang, Xin Zhao, Rong Zhang
BACKGROUND: Simvastatin may alleviate the intestinal barrier dysfunction induced by sepsis. This study aimed to investigate the role of the Ras homolog (Rho)/Rho-associated coiled-coil forming protein kinase (ROCK) signaling pathway in the intestinal barrier of simvastatin-treated rats with sepsis. MATERIALS AND METHODS: Male Wistar rats were pretreated with simvastatin (0.2 μg/g of body weight) for 1 week before cecal ligation and puncture. Twenty-four hours after cecal ligation and puncture, the condition of bacterial translocation was evaluated...
December 2018: Journal of Surgical Research
Tejinder Pal Khaket, Sun Chul Kang, Tapan Kumar Mukherjee
The receptor for advanced glycation end products (RAGE) is a multi-ligand pattern recognition receptor that is highly expressed in lung epithelial cells. It helps alveolar epithelial cells to maintain their morphology and specific architecture. However, in various pathophysiological conditions, pulmonary tissues express supraphysiological level of RAGE and its ligands including advanced glycation end products, high mobility group box 1 proteins and S100 proteins. These ligands on interaction with RAGE stimulate various downstream signaling pathways that generate inflammation and oxidative stress leading to asthma, chronic obstructive pulmonary disease, lung cancers, idiopathic pulmonary fibrosis, acute lung injury, pneumonia, bronchopulmonary dysplasia, cystic fibrosis and sepsis...
November 19, 2018: Current Drug Targets
Sherehan M Ibrahim, Muhammad Y Al-Shorbagy, Dalaal M Abdallah, Hanan S El-Abhar
Zymosan, a natural compound, provokes acute peritonitis and multiple organ dysfunction that affects the kidney, beside other organs via exaggerated inflammatory response. The aim of the present study is to test the role of cholinergic anti-inflammatory pathway (CAP) in alleviating acute kidney injury (AKI) induced by zymosan in BALB/c mice, using galantamine, a cholinesterase inhibitor, known to act via α7 nicotinic acetylcholine receptor (α7 nAChR) to stimulate CAP. Galantamine verified its anti-inflammatory effect by elevating acetylcholine (ACh) level, while abating the interleukin-6/ janus kinase 2 (Y1007/1008)/ signal transducer and activator of transcription 3 (Y705) (IL-6/ pY(1007/1008)-JAK2/ pY705-STAT3) inflammatory axis, with a consequent inhibition in suppressor of cytokine signaling 3 (SOCS3)...
November 14, 2018: Scientific Reports
Pingfeng Qiu, Licheng Wang, Jianming Ni, Yunlong Zhang
OBJECTIVES: High mobility group box 1 protein (HMGB1) is an important late inflammatory mediator in the body. In recent years, studies have found that it plays an important pathogenic role in various diseases such as sepsis. However, it is unclear whether the genetic variation of the HMGB1 gene is related to the susceptibility to sepsis. This study investigated the relationship between susceptibility and outcome of the HMGB1 gene rs2249825, rs1045411, and rs1360485 single nucleotide polymorphisms (SNPs) in Chinese Han patients with sepsis...
November 10, 2018: Gene
Changzhi Li, Dong Wang, Xin Guan, Shuang Liu, Peng Su, Qingwei Li, Yue Pang
High mobility group box protein 1 (HMGB1) acts as a potent proinflammatory cytokine that involves in the pathogenesis of diverse inflammatory and infectious disorders. In previous study, we identified a homolog of HMGB1 in the Lampetra japonica(L-HMGB1), and further revealed that L-HMGB1 was able to induce the production of tumor necrosis factor-α (TNF-α) in activated human acute monocytic leukemia cells. However, the role of L-HMGB1 played in lamprey was unknown. Here, we found that L-HMGB1 was located in the cytoplasm of lamprey leukocytes and supraneural body (SB) cells...
November 10, 2018: Developmental and Comparative Immunology
Meegan Justice, Autumn Ferrugia, Joshua Beidler, Jerrold C Penprase, Patricia Cintora, Danielle Erwin, Octavio Medrano, Susan M Brasser, Mee Young Hong
Aims: Epidemiological studies and experimental data from rodent models have reported a non-linear relationship between consumption of alcohol and cardiovascular disease (CVD) risk that suggests that light-to-moderate drinking as opposed to excessive consumption may provide some cardiovascular benefits. The present study examined potential mechanisms by which moderate alcohol consumption may provide a protective effect against CVD. Short summary: Wistar rats exposed for 3 months to a 20% ethanol intermittent-access voluntary drinking paradigm displayed a reduction in epididymal fat, blood glucose and non-HDL and total cholesterol...
November 13, 2018: Alcohol and Alcoholism: International Journal of the Medical Council on Alcoholism
Asmaa A Alsousi, Orisa J Igwe
Reactive oxygen species (ROS) are implicated to play a role in initiating rheumatoid arthritis (RA) pathogenesis. We have investigated the mechanism(s) by which essential redox-active trace metals (RATM) may induce cell proliferation and cell death in rabbit synovial fibroblasts. These fibroblast-like synovial (FLS) cells, which express Toll-like receptor 4 (TLR4), were used as a model system that plays a role in potentially initiating RA through oxidative stress. Potassium peroxychromate (PPC, [Cr5+ ]), ferrous chloride (FeCl2 , [Fe2+ ]), and cuprous chloride (CuCl, [Cu+ ]) in the indicated valency states were used as exogenous pro-oxidants that can induce oxidative stress through TLR4 coupled activation that also causes HMGB1 release...
November 6, 2018: Experimental Cell Research
Yu Guo, Zheng Xiao, Yanan Wang, Weihua Yao, Shun Liao, Bo Yu, Jianqiang Zhang, Yanxiang Zhang, Bing Zheng, Boxu Ren, Quan Gong
Type 1 diabetes (T1D) is an autoimmune disease characterized by the immune cell-mediated progressive destruction of pancreatic β-cells. High-mobility group box 1 protein (HMGB1) has been recognized as a potential immune mediator to enhance the development of T1D. So we speculated that HMGB1 inhibitors could have anti-diabetic effect. Sodium butyrate is a short fatty acid derivative possessing anti-inflammatory activity by inhibiting HMGB1. In the current study, we evaluated the effects of sodium butyrate in streptozotocin (STZ)-induced T1D mice model...
2018: Frontiers in Endocrinology
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