Read by QxMD icon Read

Lignocellulosic fermentation

Amandine Gales, Lucile Chatellard, Maider Abadie, Anaïs Bonnafous, Lucas Auer, Hélène Carrère, Jean-Jacques Godon, Guillermina Hernandez-Raquet, Claire Dumas
Microbial consortia producing specific enzymatic cocktails are present in the gut of phytophagous and xylophagous insects; they are known to be the most efficient ecosystems to degrade lignocellulose. Here, the ability of these consortia to degrade ex vivo lignocellulosic biomass in anaerobic bioreactors was characterized in term of bioprocess performances, enzymatic activities and bacterial community structure. In a preliminary screening, guts of Ergates faber (beetle), Potosia cuprea (chafer), Gromphadorrhina portentosa (cockroach), Locusta migratoria (locust), and Gryllus bimaculatus (cricket) were inoculated in anaerobic batch reactors, in presence of grounded wheat straw at neutral pH...
2018: Frontiers in Microbiology
Sugitha Thankappan, Sujatha Kandasamy, Beslin Joshi, Ksenia N Sorokina, Oxana P Taran, Sivakumar Uthandi
The harnessing of biocatalysts from extreme environment hot spring niche for biomass conversion is significant and promising owing to the special characteristics of extremozymes attributed by intriguing biogeochemistry and extreme conditions of these environments. Hence, in the present study 38 bacterial isolates obtained from hot springs of Manikaran (~ 95 °C), Kalath (~ 50 °C) and Vasist (~ 65 °C) of Himachal Pradesh were screened for glycosyl hydrolases by in situ enrichment technique using lignocellulosic biomass (LCB)...
October 15, 2018: AMB Express
Yuejiao Xie, Qing Hu, Guodong Feng, Xu Jiang, Jinlong Hu, Mingxiong He, Guoquan Hu, Shumiao Zhao, Yunxiang Liang, Zhiyong Ruan, Nan Peng
Phenolic inhibitors generated during alkaline pretreatment of lignocellulosic biomasses significantly hinder bacterial growth and subsequent biofuel and biochemical production. Water rinsing is an efficient method for removing these compounds. Nevertheless, this method often generates a great amount of wastewater, and leads to the loss of solid fiber particles and fermentable sugars. Kurthia huakuii LAM0618T , a recently identified microorganism, was herein shown to be able to efficiently transform phenolic compounds (syringaldehyde, hydroxybenzaldehyde, and vanillin) into less toxic acids...
October 12, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Shalley Sharma, Eldho Varghese, Anju Arora, K N Singh, Surender Singh, Lata Nain, Debarati Paul
Economics of ethanol production from lignocellulosic biomass depends on complete utilization of constituent carbohydrates and efficient fermentation of mixed sugars present in biomass hydrolysates. Saccharomyces cerevisiae , the commercial strain for ethanol production uses only glucose while pentoses remain unused. Recombinant strains capable of utilizing pentoses have been engineered but with limited success. Recently, presence of endogenous pentose assimilation pathway in S. cerevisiae was reported. On the contrary, evolutionary engineering of native xylose assimilating strains is promising approach...
2018: Frontiers in Bioengineering and Biotechnology
Elvira Sgobba, Luisa Blöbaum, Volker F Wendisch
Corynebacterium glutamicum is used for the million-ton-scale production of food and feed amino acids such as L-glutamate and L-lysine and has been engineered for production of carotenoids such as lycopene. These fermentation processes are based on sugars present in molasses and starch hydrolysates. Due to competing uses of starch and sugars in human nutrition, this bacterium has been engineered for utilization of alternative feedstocks, for example, pentose sugars present in lignocellulosic and hexosamines such as glucosamine (GlcN) and N -acetyl-D-glucosamine (GlcNAc)...
2018: Frontiers in Microbiology
Benoit J Kunath, Francesco Delogu, Adrian E Naas, Magnus Ø Arntzen, Vincent G H Eijsink, Bernard Henrissat, Torgeir R Hvidsten, Phillip B Pope
Microbial communities that degrade lignocellulosic biomass are typified by high levels of species- and strain-level complexity, as well as synergistic interactions between both cellulolytic and non-cellulolytic microorganisms. Coprothermobacter proteolyticus frequently dominates thermophilic, lignocellulose-degrading communities with wide geographical distribution, which is in contrast to reports that it ferments proteinaceous substrates and is incapable of polysaccharide hydrolysis. Here we deconvolute a highly efficient cellulose-degrading consortium (SEM1b) that is co-dominated by Clostridium (Ruminiclostridium) thermocellum and multiple heterogenic strains affiliated to C...
October 12, 2018: ISME Journal
E Hosseini Koupaie, S Dahadha, A A Bazyar Lakeh, A Azizi, E Elbeshbishy
The rapid depletion of natural resources and the environmental concerns associated with the use of fossil fuels as the main source of global energy is leading to an increased interest in alternative and renewable energy sources. Particular interest has been given to the lignocellulosic biomass as the most abundant source of organic matter with a potential of being utilized for energy recovery. Different approaches have been applied to convert the lignocellulosic biomass to energy products including anaerobic digestion (AD), fermentation, combustion, pyrolysis, and gasification...
October 9, 2018: Journal of Environmental Management
Hanyu Wang, Qian Li, Xiaolin Kuang, Difan Xiao, Xuebing Han, Xiangdong Hu, Xi Li, Menggen Ma
Bioconversion of lignocellulosic biomass to high-value bioproducts by fermentative microorganisms has drawn extensive attentions worldwide. Lignocellulosic biomass cannot be efficiently utilized by microorganisms, such as Saccharomyces cerevisiae, but has to be pretreated prior to fermentation. Aldehyde compounds, as the by-products generated in the pretreatment process of lignocellulosic biomass, are considered as the most important toxic inhibitors to S. cerevisiae cells for their growth and fermentation...
October 10, 2018: Applied Microbiology and Biotechnology
Alaa E Rabee, Robert J Forster, Chijioke O Elekwachi, Kkaled Z Kewan, Ebrahim A Sabra, Safinaze M Shawket, Hoda A Mahrous, Omaima A Khamiss
Anaerobic fungi colonize the rumen and degrade cellulose and hemicellulose, which enable them to be key players in the lignocellulose fermentation. Consequently, an expansion of knowledge about rumen fungi could increase animal productivity, utilization of lignified forages like alfalfa hay, and enhance fibrolytic enzymes production. Here, we used an Internal Transcribed Spacer 1 (ITS1) clone library to investigate the anaerobic rumen fungi in camel and to investigate their ability to produce cellulase and xylanase in vitro...
October 10, 2018: Journal of Basic Microbiology
Chirom Aarti, Ameer Khusro, Paul Agastian
In this context, carboxymethyl cellulase (CMCase) production from Glutamicibacter arilaitensis strain ALA4 was initially optimized by one factor at a time (OFAT) method using goat dung as proficient feedstock. Two-level full factorial design (25 factorial matrix) using first-order polynomial model revealed the significant (p < 0.05) influence of pH, moisture, and peptone on CMCase activity. Central composite design at N = 20 was further taken into account using a second-order polynomial equation, and thereby liberated maximum CMCase activity of 4925...
October 10, 2018: Preparative Biochemistry & Biotechnology
Yuanyuan Zhu, Lu Wu, Junjun Zhu, Yong Xu, Shiyuan Yu
To obtain a global insight into the dynamic protein expression pattern in Pichia stipitis during xylose fermentation in the presence of three representative inhibitors (acetic acid, vanillin, and 5-hydroxymethylfurfural), proteins were extracted for quantitative proteomic analysis using 8-plex isobaric tag for relative and absolute quantitation (iTRAQ) on a liquid chromatography-mass/mass spectrometry (LC-MS/MS) instrument. Interestingly, aconitase (Aco1p) and NAD-isocitrate dehydrogenase (Idh1p), were upregulated during the middle exponential phase in the presence of the three inhibitors during tricarboxylic acid (TCA) cycle...
October 8, 2018: FEMS Microbiology Letters
Pardeep Kumar Sadh, Suresh Kumar, Prince Chawla, Joginder Singh Duhan
A large number of by-products or wastes are produced worldwide through various food industries. These wastes cause a serious disposable problem with the environment. So, now a day's different approaches are used for alternative use of these wastes because these by-products are an excellent source of various bioactive components such as polyphenols, flavonoids, caffeine, carotenoids, creatine, and polysaccharides etc. which are beneficial for human health. Furthermore, the composition of these wastes depends on the source or type of waste...
October 8, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
Daiana Wischral, Johanna Méndez Arias, Luiz Felipe Modesto, Douglas de França Passos, Nei Pereira
Lactic acid, traditionally obtained through fermentation process, presents numerous applications in different industrial segments, including production of biodegradable polylactic acid (PLA). Development of low cost substrate fermentations could improve economic viability of lactic acid production, through the use of agricultural residues as lignocellulosic biomass. Studies regarding the use of sugarcane bagasse hydrolysates for lactic acid production by Lactobacillus spp. are reported. First, five strains of Lactobacillus spp...
October 8, 2018: Biotechnology Progress
Phuong Tran Nguyen Hoang, Ja Kyong Ko, Gyeongtaek Gong, Youngsoon Um, Sun-Mi Lee
Background: Engineered strains of Saccharomyces cerevisiae have significantly improved the prospects of biorefinery by improving the bioconversion yields in lignocellulosic bioethanol production and expanding the product profiles to include advanced biofuels and chemicals. However, the lignocellulosic biorefinery concept has not been fully applied using engineered strains in which either xylose utilization or advanced biofuel/chemical production pathways have been upgraded separately...
2018: Biotechnology for Biofuels
Jungang Zhou, Peixia Zhu, Xiaoyue Hu, Hong Lu, Yao Yu
Background: Taking into account its thermotolerance, high growth rate, and broad substrate spectrum, Kluyveromyces marxianus can be considered an ideal consolidated bioprocessing (CBP). A major obstacle to ethanol production using K. marxianus is the low production of lignocellulolytic enzymes, which reduces the cellulose hydrolysis and ethanol production. Thus, further improvement of enzyme expression and secretion is essential. Results: To improve the expression of lignocellulolytic enzymes, the inulinase promoter and signal sequence from K...
2018: Biotechnology for Biofuels
Yukai Suo, Zhengping Liao, Chunyun Qu, Hongxin Fu, Jufang Wang
Resistance to furan derivatives and phenolic compounds plays an important role in the use of lignocellulosic biomass for biological production of chemicals and fuels. This study confirmed that expression of short-chain dehydrogenase/reductase (SDR) from Clostridium beijerinckii NCIMB 8052 significantly improved the tolerance of C. tyrobutyricum to furfural due to the enhanced activity for furfural reduction. And on this basis, co-expression of SDR and heat shock chaperones GroESL could simultaneously enhance the tolerance of C...
September 19, 2018: Bioresource Technology
Vinoth Kumar Ponnusamy, Dinh Duc Nguyen, Jeyaprakash Dharmaraja, Sutha Shobana, J Rajesh Banu, Rijuta Ganesh Saratale, Soon Woong Chang, Gopalakrishnan Kumar
In recent years, lignin valorization is commercially an important and advanced sustainable process for lignocellulosic biomass-based industries, primarily through the depolymerization path. The conversion of the lignin moieties into biofuels and other high value-added products are still challenging to the researchers due to the heterogeneity and complex structure of lignin-containing biomass. Besides, the involvement of different microorganisms that carries varying metabolic and enzymatic complex systems towards degradation and conversion of the lignin moieties also discussed...
September 18, 2018: Bioresource Technology
Zeyu Chen, Gang Liu, Jian Zhang, Jie Bao
l-Lysine is a commodity amino acid produced from starch feedstock. Various alternative feedstocks had been used for l-lysine production, but the yield was very low. This study took the first preliminary investigation on l-lysine production from lignocellulose for the replacement of food-crop starch. Corn stover was dry acid pretreated and biodetoxified, then used for enzymatic hydrolysis and l-lysine fermentation by an industrial Corynebacterium glutamicum strain. Various fermentation parameters, nutrient additions, and operation variables were applied and finally 33...
September 20, 2018: Bioresource Technology
María Hijosa-Valsero, Jerson Garita-Cambronero, Ana I Paniagua-García, Rebeca Díez-Antolínez
BACKGROUND: Coffee silverskin, a by-product from coffee roasting industries, was evaluated as a feedstock for biobutanol production by acetone-butanol-ethanol fermentation. This lignocellulosic biomass contained approximately 30% total carbohydrates and 30% lignin. Coffee silverskin was subjected to autohydrolysis at 170 °C during 20 min, with a biomass-to-solvent ratio of 20%, and a subsequent enzymatic hydrolysis with commercial enzymes in order to release simple sugars. The fermentability of the hydrolysate was assessed with four solventogenic strains from the genus Clostridium...
September 27, 2018: Microbial Cell Factories
Pornkamol Unrean, Jochem Gätgens, Bianca Klein, Stephan Noack, Verawat Champreda
A robust cell factory that can tolerate combined inhibitory lignocellulosic compounds is essential for the cost-effective lignocellulose-based production of second-generation bioethanol and other bulk chemicals. Following high-throughput phenotyping of a yeast genomic overexpression library, we identified a Saccharomyces cerevisiae mutant (denoted AFb.01) with improved growth and fermentation performance under combined toxicity of acetic acid and furfural. AFb.01 carries overexpression of TRX1, which encodes for thioredoxin, a cellular redox machinery...
September 25, 2018: FEMS Yeast Research
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"