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Lignocellulosic fermentation

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https://www.readbyqxmd.com/read/30553083/enhanced-volatile-fatty-acids-production-from-waste-activated-sludge-anaerobic-fermentation-by-adding-tofu-residue
#1
Xiaoding Huang, Jianwei Zhao, Qiuxiang Xu, Xiaoming Li, Dongbo Wang, Qi Yang, Yang Liu, Ziletao Tao
In this study, an economical and eco-friendly strategy (i.e., adding tofu residue (TR) into waste activated sludge (WAS)) to enhance volatile fatty acid (VFA) production was reported. Experimental results indicated that the maximal VFA yield at T/W ratio (TR/WAS, the ratio of the volatile suspended solids (VSS)) of 0.64 on 5 d was 240.8 mg COD/g VSS, which was 10.2 and 1.1-fold of that in sole WAS and sole TR, respectively. The feasible fermentation time was shortened by 2 days, as compared with sole WAS or sole TR...
December 7, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/30552624/adsorption-study-of-acid-soluble-lignin-removal-from-sugarcane-bagasse-hydrolysate-by-a-self-synthesized-resin-for-lipid-production
#2
Xue-Fang Chen, Li-Quan Zhang, Chao Huang, Lian Xiong, Hai-Long Li, Can Wang, Cheng Zhao, Qian-Lin Huang, Xin-de Chen
An adsorption resin CX-6 was synthesized and used for acid soluble lignin (ASL) removal from sugarcane bagasse hydrolysate (SCBH). The adsorption conditions of pH value, amount of adsorbent, initial ASL concentration, and temperature on ASL adsorption were discussed. The results showed the adsorption capacity of ASL was negatively affected by increasing temperature, solution pH, and adsorbent dose, and was positively affected by increasing initial concentration. The maximum adsorption capacity of ASL was 135...
December 15, 2018: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/30541780/the-phylogenomic-diversity-of-herbivore-associated-fibrobacter-spp-is-correlated-to-lignocellulose-degrading-potential
#3
Anthony P Neumann, Garret Suen
Members of the genus Fibrobacter are cellulose-degrading bacteria and common constituents of the gastrointestinal microbiota of herbivores. Although considerable phylogenetic diversity is observed among members of this group, few functional differences explaining the distinct ecological distributions of specific phylotypes have been described. In this study, we sequenced and performed a comparative analysis of whole genomes from 38 novel Fibrobacter strains against the type strains for the two formally described Fibrobacter species F...
December 12, 2018: MSphere
https://www.readbyqxmd.com/read/30541521/simultaneous-enzymatic-saccharification-and-comminution-for-the-valorization-of-lignocellulosic-biomass-toward-natural-products
#4
Ronald R Navarro, Yuichiro Otsuka, Masanobu Nojiri, Shigehiro Ishizuka, Masaya Nakamura, Kazuhiro Shikinaka, Kenji Matsuo, Kei Sasaki, Ken Sasaki, Kazuhide Kimbara, Yutaka Nakashimada, Junichi Kato
BACKGROUND: Large-scale processing of lignocellulosics for glucose production generally relies on high temperature and acidic or alkaline conditions. However, extreme conditions produce chemical contaminants that complicate downstream processing. A method that mainly rely on mechanical and enzymatic reaction completely averts such problem and generates unmodified lignin. Products from this process could find novel applications in the chemicals, feed and food industry. But a large-scale system suitable for this purpose is yet to be developed...
December 12, 2018: BMC Biotechnology
https://www.readbyqxmd.com/read/30534201/metabolic-engineering-of-the-thermophilic-filamentous-fungus-myceliophthora-thermophila-to-produce-fumaric-acid
#5
Shuying Gu, Jingen Li, Bingchen Chen, Tao Sun, Qian Liu, Dongguang Xiao, Chaoguang Tian
Background: Fumaric acid is widely used in food and pharmaceutical industries and is recognized as a versatile industrial chemical feedstock. Increasing concerns about energy and environmental problems have resulted in a focus on fumaric acid production by microbial fermentation via bioconversion of renewable feedstocks. Filamentous fungi are the predominant microorganisms used to produce organic acids, including fumaric acid, and most studies to date have focused on Rhizopus species...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/30529238/tailor-made-trees-engineering-lignin-for-ease-of-processing-and-tomorrow-s-bioeconomy
#6
REVIEW
Elizabeth L Mahon, Shawn D Mansfield
Lignocellulosic biomass represents an abundant source of cellulosic fibres and fermentable sugars. However, lignin, a polyphenolic constituent of secondary-thickened plant cell walls significantly contributes to biomass recalcitrance during industrial processing. Efforts to reduce plant total lignin content through genetic engineering have improved processing efficiency, but often incur an agronomic penalty. Alternatively, modifications that alter the composition of lignin and/or its interaction with other cell wall polymers display improved processing efficiency without compromising biomass yield...
December 4, 2018: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/30510474/bioethanol-production-from-renewable-raw-materials-and-its-separation-and-purification-a-review
#7
REVIEW
Arijana Bušić, Nenad Marđetko, Semjon Kundas, Galina Morzak, Halina Belskaya, Mirela Ivančić Šantek, Draženka Komes, Srđan Novak, Božidar Šantek
Production of biofuels from renewable feedstocks has captured considerable scientific attention since they could be used to supply energy and alternative fuels. Bioethanol is one of the most interesting biofuels due to its positive impact on the environment. Currently, it is mostly produced from sugar- and starch-containing raw materials. However, various available types of lignocellulosic biomass such as agricultural and forestry residues, and herbaceous energy crops could serve as feedstocks for the production of bioethanol, energy, heat and value-added chemicals...
September 2018: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/30505946/metatranscriptomic-and-thermodynamic-insights-into-medium-chain-fatty-acid-production-using-an-anaerobic-microbiome
#8
Matthew J Scarborough, Christopher E Lawson, Joshua J Hamilton, Timothy J Donohue, Daniel R Noguera
Biomanufacturing from renewable feedstocks can offset fossil fuel-based chemical production. One potential biomanufacturing strategy is production of medium-chain fatty acids (MCFA) from organic feedstocks using either pure cultures or microbiomes. While the set of microbes in a microbiome can often metabolize organic materials of greater diversity than a single species can and while the role of specific species may be known, knowledge of the carbon and energy flow within and between organisms in MCFA-producing microbiomes is only now starting to emerge...
November 2018: MSystems
https://www.readbyqxmd.com/read/30502643/ethanol-fermentation-from-non-detoxified-lignocellulose-hydrolysate-by-a-multi-stress-tolerant-yeast-candida-glycerinogenes-mutant
#9
Meilin Zhao, Dingchang Shi, Xinyao Lu, Hong Zong, Bin Zhuge, Hao Ji
The aim of this work was to study ethanol fermentation properties of the robust mutant Candida glycerinogenes UG21 from non-detoxified lignocellulose hydrolysate. C. glycerinogenes UG21 with high tolerance to elevated temperature, acetic acid, and furfural was obtained and applied in lignocellulose-based ethanol production. C. glycerinogenes UG21 exhibited highly-efficient degradation ability to furfural. High levels of acetic acid and furfural inhibited cell growths and ethanol production of Saccharomyces cerevisiae ZWA46 and industrial Angel yeast but had a slight impact on biomass and ethanol titer of C...
November 14, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/30502607/benefit-of-solid-liquid-separation-on-volatile-fatty-acid-production-from-grass-clipping-with-ultrasound-calcium-hydroxide-pretreatment
#10
Siqi Wang, Xue Tao, Guangming Zhang, Panyue Zhang, Hongjie Wang, Junpei Ye, Fan Li, Qian Zhang, Mohammad Nabi
Ultrasound-calcium hydroxide (US-Ca(OH)2 ) pretreatment effectively enhanced volatile fatty acid (VFA) production from lignocellulosic biomass. In this paper, solid and liquid fraction of pretreated grass clipping was for the first time separately fermented in order to improve organic recovery from liquid fraction and reduce inhibition due to alkaline pretreatment. The total VFA yield and VS removal reached 515 mg/g TS and 59.7% after solid-liquid separation, exhibiting an increase of 116.7% and 91.9% comparing to that of mixture sample...
November 22, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/30498977/evolutionary-engineered-candida-intermedia-exhibits-improved-xylose-utilization-and-robustness-to-lignocellulose-derived-inhibitors-and-ethanol
#11
Antonio D Moreno, Antonella Carbone, Rosita Pavone, Lisbeth Olsson, Cecilia Geijer
The development of robust microorganisms that can efficiently ferment both glucose and xylose represents one of the major challenges in achieving a cost-effective lignocellulosic bioethanol production. Candida intermedia is a non-conventional, xylose-utilizing yeast species with a high-capacity xylose transport system. The natural ability of C. intermedia to produce ethanol from xylose makes it attractive as a non-GMO alternative for lignocellulosic biomass conversion in biorefineries. We have evaluated the fermentation capacity and the tolerance to lignocellulose-derived inhibitors and the end product, ethanol, of the C...
November 29, 2018: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/30498944/cow-manure-as-a-lignocellulosic-substrate-for-fungal-cellulase-expression-and-bioethanol-production
#12
Qin Yan, Xinli Liu, Yanan Wang, Hongxing Li, Zhigang Li, Lin Zhou, Yinbo Qu, Zhonghai Li, Xiaoming Bao
Conversion of various lignocellulosic materials into bioethanol is growing in demand but greatly depends on feedstock availability. Dairy cow manure is an agricultural waste widely distributed worldwide. This study investigated the induction of cellulases by cow manure and the conversion of cow manure materials into lignocellulosic ethanol. Alkaline NaOH pretreatment improved the accessibility of cow manure lignocellulose to enzymes followed by enzymatic hydrolysis using Penicillium oxalicum cellulases. The ethanol yields from pretreated cow manure and anaerobically digested cow manure were 0...
November 29, 2018: AMB Express
https://www.readbyqxmd.com/read/30479661/developing-fast-enzyme-recycling-strategy-through-elucidating-enzyme-adsorption-kinetics-on-alkali-and-acid-pretreated-corn-stover
#13
Ye Yuan, Rui Zhai, Ying Li, Xiangxue Chen, Mingjie Jin
Background: Although various pre-treatment methods have been developed to disrupt the structure of lignocellulosic biomass, high dosage of cellulases is still required to hydrolyze lignocellulose to fermentable sugars. Enzyme recycling via recycling unhydrolyzed solids after enzymatic hydrolysis is a promising strategy to reduce enzyme loading for production of cellulosic ethanol. Results: To develop effective enzyme recycling method via recycling unhydrolyzed solids, this work investigated both enzymatic hydrolysis kinetics and enzyme adsorption kinetics on dilute acid and dilute alkali pre-treated corn stover (CS)...
2018: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/30478751/solid-state-fermentation-of-distilled-dried-grain-with-solubles-with-probiotics-for-degrading-lignocellulose-and-upgrading-nutrient-utilization
#14
Cheng Wang, Weifa Su, Yu Zhang, Lihong Hao, Fengqin Wang, Zeqing Lu, Jian Zhao, Xuelian Liu, Yizhen Wang
Solid-state fermentation (SSF) was carried out in this study to improve the nutritional digestibility of two types of distilled dried grain with solubles (DDGS) by inoculating probiotic combinations. The fermented DDGS (FDDGS) contained more crude protein, small peptides and total amino acids than did unfermented DDGS. The concentrations of fiber indexes significantly declined after fermentation. The amounts of probiotics, enzymes and organic acids were significantly improved after fermentation. Microscopy revealed that SSF disrupted the surface structure and increased small fragments of DDGS substrate, thereby facilitating in vitro digestibility of FDDGS...
November 26, 2018: AMB Express
https://www.readbyqxmd.com/read/30478728/transcriptome-and-metabolome-analysis-of-pichia-stipitis-to-three-representative-lignocellulosic-inhibitors
#15
Yuanyuan Zhu, Lu Wu, Junjun Zhu, Yong Xu, Shiyuan Yu
During the bioconversion of xylose to ethanol, Pichia stipitis cells are often inhibited by substances generated in the lignocellulosic hydrolysate. However, the response mechanism of P. stipitis to inhibitors has not been completely understood till date. With this aim, integrated transcriptomic and metabolomic analyses were performed on P. stipitis to investigate the interactive effects of three representative inhibitors [vanillin, 5-hydroxymethylfurfural (5-HMF), and acetic acid] present in lignocellulosic hydrolysates...
November 26, 2018: Archives of Microbiology
https://www.readbyqxmd.com/read/30478228/-butyrivibrio-hungatei-mb2003-competes-effectively-for-soluble-sugars-released-by-butyrivibrio-proteoclasticus-b316-t-from-growth-on-xylan-or-pectin
#16
Nikola Palevich, William J Kelly, Siva Ganesh, Jasna Rakonjac, Graeme T Attwood
Rumen bacterial species belonging to the genus Butyrivibrio are important degraders of plant polysaccharides, particularly hemicelluloses (arabinoxylans) and pectin. Currently, four species are recognized which have very similar substrate utilization profiles, but little is known about how these microorganisms are able to co-exist in the rumen. To investigate this question, Butyrivibrio hungatei MB2003 and Butyrivibrio proteoclasticus B316T were grown alone or in co-culture on xylan or pectin, and their growth, release of sugars, fermentation end products and transcriptomes were examined...
November 26, 2018: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/30477292/effects-of-lactic-acid-bacteria-and-molasses-on-fermentation-dynamics-structural-and-nonstructural-carbohydrate-composition-and-in-vitro-ruminal-fermentation-of-rice-straw-silage
#17
Jie Zhao, Zhihao Dong, Junfeng Li, Lei Chen, Yunfeng Bai, Yushan Jia, Tao Shao
Objectve: This study was to evaluate the fermentation dynamics, structural and nonstructural carbohydrate composition and in vitro gas production of rice straw ensiled with lactic acid bacteria (LAB) and molasses. Methods: Fresh rice straw was ensiled in 1-L laboratory silos with no additive control (C), Lactobacillus plantarum (L), molasses (M) and molasses + L. plantarum (ML) for 6, 15, 30 and 60 days. After storage, the silages were subjected to microbial and chemical analyses as well as the further in vitro fermentation trial...
November 27, 2018: Asian-Australasian Journal of Animal Sciences
https://www.readbyqxmd.com/read/30471644/lignin-first-biomass-fractionation-using-a-hybrid-organosolv-steam-explosion-pretreatment-technology-improves-the-saccharification-and-fermentability-of-spruce-biomass
#18
Leonidas Matsakas, Vijayendran Raghavendran, Olga Yakimenko, Gustav Persson, Eva Olsson, Ulrika Rova, Lisbeth Olsson, Paul Christakopoulos
For a transition to a sustainable society, fuels, chemicals, and materials should be produced from renewable resources. Lignocellulosic biomass constitutes an abundant and renewable feedstock; however, its successful application in a biorefinery requires efficient fractionation into its components; cellulose, hemicellulose and lignin. Here, we demonstrate that a newly established hybrid organosolv - steam explosion pretreatment can effectively fractionate spruce biomass to yield pretreated solids with high cellulose (72% w/w) and low lignin (delignification up to 79...
November 15, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/30471359/yeast-chemogenomic-screen-identifies-distinct-metabolic-pathways-required-to-tolerate-exposure-to-phenolic-fermentation-inhibitors-ferulic-acid-4-hydroxybenzoic-acid-and-coniferyl-aldehyde
#19
Eugene Fletcher, Kai Gao, Kevin Mercurio, Mariam Ali, Kristin Baetz
The conversion of plant material into biofuels and high value products is a two-step process of hydrolysing plant lignocellulose and next fermenting the sugars produced. However, lignocellulosic hydrolysis not only frees sugars for fermentation it simultaneously generates toxic chemicals, including phenolic compounds which severely inhibit yeast fermentation. To understand the molecular basis of phenolic compound toxicity, we performed genome-wide chemogenomic screens in Saccharomyces cerevisiae to identify deletion mutants that were either hypersensitive or resistant to three common phenolic compounds found in hydrolysates: coniferyl aldehyde, ferulic acid and 4-hydroxybenzoic acid...
November 21, 2018: Metabolic Engineering
https://www.readbyqxmd.com/read/30469134/enhanced-butyric-acid-production-using-mixed-biomass-of-brown-algae-and-rice-straw-by-clostridium-tyrobutyricum-atcc25755
#20
Hyun Ju Oh, Ki-Yeon Kim, Kyung Min Lee, Sun-Mi Lee, Gyeongtaek Gong, Min-Kyu Oh, Youngsoon Um
A brown alga Saccharina japonica and rice straw are attractive feedstock for microbial butyric acid production. However, inefficient fermentation of mannitol (a dominant component in S. japonica) and toxicity of inhibitors in lignocellulosic hydrolysate are limitations. This study demonstrated that mixed biomass with S. japonica and rice straw was effective in butyric acid production over those restrictions. Mannitol was consumed only when acetic acid was present. Notably, acetic acid was not produced but consumed along with mannitol...
November 10, 2018: Bioresource Technology
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