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https://www.readbyqxmd.com/read/30013186/more-expansive-gene-transfer-to-the-rat-cns-aav-php-eb-vector-dose-response-and-comparison-to-aav-php-b
#1
Robert D Dayton, Mychal S Grames, Ronald L Klein
Engineered recombinant adeno-associated virus (AAV) vectors have advanced the transduction of neurons in the CNS on an expansive, wide-scale basis since the papers first using AAV9 for this purpose. Wide-scale CNS expression is relevant to gene therapy as well as indispensable for basic studies such as disease modeling. For example, the wide-scale gene transfer approach could expedite hypothesis testing in vivo relative to the generation of germ-line transgenic mice for all of the genes of interest. Wide-scale gene transfer is more efficient in neonates than in adults, so improving gene transfer efficiency in adults is an important goal...
July 16, 2018: Gene Therapy
https://www.readbyqxmd.com/read/29985867/hip-power-and-stiff-knee-gait-a-tool-for-identifying-appropriate-candidates-for-rectus-transfer
#2
Brunno L Moreira, Tasos Karakostas, Luciano S Dias
BACKGROUND: Rectus femoris transfer (RFT) is performed in children with cerebral palsy to treat stiff-knee gait. However, the results are variable in part because there is no gold standard to identify appropriate candidates. Hip flexion kinematics and kinetics are important factors in the determination of peak knee flexion during gait. This study aimed to evaluate whether a kinetic preoperative analysis of hip power can be a predictor of RFT outcomes and determine its usability for identifying appropriate candidates for the procedure...
July 6, 2018: Journal of Pediatric Orthopedics
https://www.readbyqxmd.com/read/29954828/comparative-effectiveness-of-treatment-options-for-plantar-heel-pain-a-systematic-review-with-network-meta-analysis
#3
REVIEW
Opeyemi O Babatunde, Amardeep Legha, Chris Littlewood, Linda S Chesterton, Martin J Thomas, Hylton B Menz, Danielle van der Windt, Edward Roddy
OBJECTIVE: To evaluate the comparative effectiveness of current treatment options for plantar heel pain (PHP). DESIGN: Systematic review and network meta-analysis (NMA). DATA SOURCES: Medline, EMBASE, CINAHL, AMED, PEDro, Cochrane Database, Web of Science and WHO Clinical Trials Platform were searched from their inception until January 2018. STUDY SELECTION: Randomised controlled trials (RCTs) of adults with PHP investigating common treatments (ie, corticosteroid injection, non-steroidal anti-inflammatory drugs, therapeutic exercise, orthoses and/or extracorporeal shockwave therapy (ESWT)) compared with each other or a no treatment, placebo/sham control...
June 28, 2018: British Journal of Sports Medicine
https://www.readbyqxmd.com/read/29523880/widespread-transduction-of-astrocytes-and-neurons-in-the-mouse-central-nervous-system-after-systemic-delivery-of-a-self-complementary-aav-php-b-vector
#4
Melvin Y Rincon, Filip de Vin, Sandra I Duqué, Shelly Fripont, Stephanie A Castaldo, Jessica Bouhuijzen-Wenger, Matthew G Holt
Until recently, adeno-associated virus 9 (AAV9) was considered the AAV serotype most effective in crossing the blood-brain barrier (BBB) and transducing cells of the central nervous system (CNS), following systemic injection. However, a newly engineered capsid, AAV-PHP.B, is reported to cross the BBB at even higher efficiency. We investigated how much we could boost CNS transgene expression by using AAV-PHP.B carrying a self-complementary (sc) genome. To allow comparison, 6 weeks old C57BL/6 mice received intravenous injections of scAAV2/9-GFP or scAAV2/PHP...
April 2018: Gene Therapy
https://www.readbyqxmd.com/read/29522153/nessie-a-tool-for-the-identification-of-approximate-dna-sequence-symmetries
#5
Michele Berselli, Enrico Lavezzo, Stefano Toppo
Motivation: Non-B DNA conformations play an important role in genomic rearrangements, structural three-dimensional organization and gene regulation. Many non-B DNA structures show symmetrical properties as palindromes and mirrors that can form hairpins, cruciform structures or triplexes. A comprehensive tool, capable to perform a fast genome wide search for exact and degenerate symmetrical patterns, is needed for further investigating nucleotide tracts potentially forming non-B DNA structures...
July 15, 2018: Bioinformatics
https://www.readbyqxmd.com/read/29422013/first-and-second-trimester-urinary-metabolic-profiles-and-fetal-growth-restriction-an-exploratory-nested-case-control-study-within-the-infant-development-and-environment-study
#6
Gauri Luthra, Ivan Vuckovic, A Bangdiwala, H Gray, J B Redmon, E S Barrett, S Sathyanarayana, R H N Nguyen, S H Swan, S Zhang, P Dzeja, S I Macura, K S Nair
BACKGROUND: Routine prenatal care fails to identify a large proportion of women at risk of fetal growth restriction (FGR). Metabolomics, the comprehensive analysis of low molecular weight molecules (metabolites) in biological samples, can provide new and earlier biomarkers of prenatal health. Recent research has suggested possible predictive first trimester urine metabolites correlating to fetal growth restriction in the third trimester. Our objective in this current study was to examine urinary metabolic profiles in the first and second trimester of pregnancy in relation to third trimester FGR in a US population from a large, multi-center cohort study of healthy pregnant women...
February 8, 2018: BMC Pregnancy and Childbirth
https://www.readbyqxmd.com/read/29379892/-epi-genotype-phenotype-analysis-in-69-japanese-patients-with-pseudohypoparathyroidism-type-i
#7
Shinichiro Sano, Akie Nakamura, Keiko Matsubara, Keisuke Nagasaki, Maki Fukami, Masayo Kagami, Tsutomu Ogata
Context: Pseudohypoparathyroidism type I (PHP-I) is divided into PHP-Ia with Albright hereditary osteodystrophy and PHP-Ib, which usually shows no Albright hereditary osteodystrophy features. Although PHP-Ia and PHP-Ib are typically caused by genetic defects involving α subunit of the stimulatory G protein (Gs α )-coding GNAS exons and methylation defects of the GNAS differentially methylated regions (DMRs) on the maternal allele, respectively, detailed phenotypic characteristics still remains to be examined...
January 1, 2018: Journal of the Endocrine Society
https://www.readbyqxmd.com/read/29374650/concurrent-pneumocystis-jirovecii-and-pulmonary-histoplasmosis-in-an-undiagnosed-hiv-patient
#8
Ahsan Wahab, Siddique Chaudhary, Mahin Khan, Susan Jane Smith
Concurrent Pneumocystis jirovecii (PJ) and pulmonary histoplasmosis (PHP) are rare in a single HIV individual. We present a challenging case of concomitant PJ and PHP in a young HIV individual. A 44-year-old man presented to the emergency department with progressive pulmonary symptoms. He was hypoxic with bilateral pulmonary opacities on chest radiograph. CT of the chest showed a geographical pattern of ground-glass attenuation. He started receiving intravenous antibiotics in addition to oral Bactrim for suspected PJ...
January 26, 2018: BMJ Case Reports
https://www.readbyqxmd.com/read/29312567/b-can-a-resource-sharing-platform-to-improve-the-operation-visualization-and-integrated-analysis-of-tcga-breast-cancer-data
#9
Can-Hong Wen, Shao-Min Ou, Xiao-Bo Guo, Chen-Feng Liu, Yan-Bo Shen, Na You, Wei-Hong Cai, Wen-Jun Shen, Xue-Qin Wang, Hai-Zhu Tan
Breast cancer is a high-risk heterogeneous disease with myriad subtypes and complicated biological features. The Cancer Genome Atlas (TCGA) breast cancer database provides researchers with the large-scale genome and clinical data via web portals and FTP services. Researchers are able to gain new insights into their related fields, and evaluate experimental discoveries with TCGA. However, it is difficult for researchers who have little experience with database and bioinformatics to access and operate on because of TCGA's complex data format and diverse files...
December 12, 2017: Oncotarget
https://www.readbyqxmd.com/read/29303947/outcomes-of-acute-partial-hospital-treatment-comparison-of-two-programs-and-a-waiting-list-control
#10
Paul B Lieberman, Rendueles Villalba, Samantha G Farris
OBJECTIVE: To investigate the effectiveness of acute, very brief partial hospital treatment, and the comparative benefits of different types of partial hospitalization programs (PHPs), this exploratory study examined changes in psychiatric symptoms and elements of cognitive functioning during an acute PHP compared with a waiting list control, and also compared such changes in 2 PHPs that treated similar patient groups but used different treatment approaches. METHODS: Patients were admitted to 2 PHPs, one utilizing cognitive-behavioral therapy and the other interpersonal and existential psychotherapy...
November 2017: Journal of Psychiatric Practice
https://www.readbyqxmd.com/read/29281027/cas9-sgrna-selective-targeting-of-the-p23h-rhodopsin-mutant-allele-for-treating-retinitis-pigmentosa-by-intravitreal-aav9-php-b-based-delivery
#11
Serena G Giannelli, Mirko Luoni, Valerio Castoldi, Luca Massimino, Tommaso Cabassi, Debora Angeloni, Gian Carlo Demontis, Letizia Leocani, Massimiliano Andreazzoli, Vania Broccoli
P23H is the most common mutation in the RHODOPSIN (RHO) gene leading to a dominant form of retinitis pigmentosa (RP), a rod photoreceptor degeneration that invariably causes vision loss. Specific disruption of the disease P23H RHO mutant while preserving the wild-type (WT) functional allele would be an invaluable therapy for this disease. However, various technologies tested in the past failed to achieve effective changes and consequently therapeutic benefits. We validated a CRISPR/Cas9 strategy to specifically inactivate the P23H RHO mutant, while preserving the WT allele in vitro...
March 1, 2018: Human Molecular Genetics
https://www.readbyqxmd.com/read/29240265/bone-mineral-density-and-its-serial-changes-are-associated-with-pth-levels-in-pseudohypoparathyroidism-type-1b-patients
#12
Xueying Chu, Yan Zhu, Ou Wang, Min Nie, Tingting Quan, Yu Xue, Wenbo Wang, Yan Jiang, Mei Li, Weibo Xia, Xiaoping Xing
Bone responsiveness to serum parathyroid hormone (PTH) in pseudohypoparathyroidism 1B (PHP1B) is controversial. Forty-eight PHP1B patients diagnosed by molecular analysis were recruited from 2000 to 2016 from the Peking Union Medical College Hospital. Fifty-five sex-matched nonsurgical hypoparathyroidism (NS-HP) patients were selected and included for comparison. Basic information, laboratory test, and dual-energy X-ray absorptiometry (DXA) results were collected. Linear regression was performed to identify independent predictors of lumbar spine (LS), femoral neck (FN), and total hip (TH) bone mineral density (BMD) Z-scores in PHP1B patients...
April 2018: Journal of Bone and Mineral Research: the Official Journal of the American Society for Bone and Mineral Research
https://www.readbyqxmd.com/read/29234333/putative-microsatellite-dna-marker-based-wheat-genomic-resource-for-varietal-improvement-and-management
#13
Sarika Jaiswal, Sonia Sheoran, Vasu Arora, Ulavappa B Angadi, Mir A Iquebal, Nishu Raghav, Bharti Aneja, Deepender Kumar, Rajender Singh, Pradeep Sharma, G P Singh, Anil Rai, Ratan Tiwari, Dinesh Kumar
Wheat fulfills 20% of global caloric requirement. World needs 60% more wheat for 9 billion population by 2050 but climate change with increasing temperature is projected to affect wheat productivity adversely. Trait improvement and management of wheat germplasm requires genomic resource. Simple Sequence Repeats (SSRs) being highly polymorphic and ubiquitously distributed in the genome, can be a marker of choice but there is no structured marker database with options to generate primer pairs for genotyping on desired chromosome/physical location...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/29175632/intravenous-administration-of-the-adeno-associated-virus-php-b-capsid-fails-to-upregulate-transduction-efficiency-in-the-marmoset-brain
#14
Yasunori Matsuzaki, Ayumu Konno, Ryuta Mochizuki, Yoichiro Shinohara, Keisuke Nitta, Yukihiro Okada, Hirokazu Hirai
Intravenous administration of adeno-associated virus (AAV)-PHP.B, a capsid variant of AAV9 containing seven amino acid insertions, results in a greater permeability of the blood brain barrier (BBB) than standard AAV9 in mice, leading to highly efficient and global transduction of the central nervous system (CNS). The present study aimed to examine whether the enhanced BBB penetrance of AAV-PHP.B observed in mice also occurs in non-human primates. Thus, a young adult (age, 1.6 years) and an old adult (age, 7...
February 5, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29118982/uneven-distribution-of-professors-and-instructors-in-medical-disciplines-dealing-with-the-four-main-chronic-non-communicable-diseases-the-case-of-the-italian-universities
#15
Giovanni Viegi, Stefano Centanni, Francesco Blasi
Background: Chronic (non-communicable) diseases (NCD) -- principally cardiovascular diseases, cancer, chronic respiratory diseases, and diabetes -- are leading causes of death and disability. There is the need to adopt a core University curriculum which let students be taught by teachers who are experts of the four main NCD, for reaching the public health goals proposed by the UN and the WHO. Methods: Our aim was to assess whether all medical students, regardless of the Italian university of enrolment, have an equal opportunity to be educated by an expert teacher in each of the four NCD...
2017: Multidisciplinary Respiratory Medicine
https://www.readbyqxmd.com/read/29017357/automated-hexahedral-meshing-of-knee-cartilage-structures-application-to-data-from-the-osteoarthritis-initiative
#16
MULTICENTER STUDY
B Rodriguez-Vila, P Sánchez-González, I Oropesa, E J Gomez, D M Pierce
We propose a fully automated methodology for hexahedral meshing of patient-specific structures of the human knee obtained from magnetic resonance images, i.e. femoral/tibial cartilages and menisci. We select eight patients from the Osteoarthritis Initiative and validate our methodology using MATLAB on a laptop computer. We obtain the patient-specific meshes in an average of three minutes, while faithfully representing the geometries with well-shaped elements. We hope to provide a fundamentally different means to test hypotheses on the mechanisms of disease progression by integrating our patient-specific FE meshes with data from individual patients...
November 2017: Computer Methods in Biomechanics and Biomedical Engineering
https://www.readbyqxmd.com/read/28981237/-hypo-and-hypercalcemia-from-diagnosis-to-treatment
#17
B Sirault
The hypercalcemia is defined as a blood calcium range above 2.6 mmol/l. Its prevalence is variable in different studies and it is comprised between 2 and 8/1000 in the general population. The primary hyperparathyroidism (PHP) and cancers are both responsible for about 80 per cent to 90 per cent of causes of hypercalcemia1. The PHP is the first ambulatory cause of hypercalcemia. Contrarily to this, the neoplastic etiology is mainly seen in the hospital settings. The other causes of the hypercalcemia are vitamin D overdose, secondary hypercalcemia due to medications intake and familial hypocalciuric hypercalcemia which will be discussed in this article...
2016: Revue Médicale de Bruxelles
https://www.readbyqxmd.com/read/28933120/-explore-anti-atherosclerotic-mechanism-of-component-compatibility-of-danshen-and-shanzha-based-on-network-pharmacology-and-cell-level
#18
Jian-Yong Zhang, Lan Wang, Ri-Xin Liang, Bin Yang
To explore the anti-atherosclerotic mechanism of active component compatibility of Danshen and Shanzha (SC121) based on network pharmacology and in vitro research validation with cell model. On one hand, according to the chemical structures and pharmacological activities of the compounds reported in Danshen and Shanzha, 5 compounds, i.e., salvianolic acid B, tanshinone ⅡA, tanshinol, epicatechin and procyanidin B2 were chosen and used for network pharmacology analysis. Then the TCMSP(http://lsp.nwsuaf.edu...
December 2016: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/28882452/aav-php-b-mediated-global-scale-expression-in-the-mouse-nervous-system-enables-gba1-gene-therapy-for-wide-protection-from-synucleinopathy
#19
Giuseppe Morabito, Serena G Giannelli, Gabriele Ordazzo, Simone Bido, Valerio Castoldi, Marzia Indrigo, Tommaso Cabassi, Stefano Cattaneo, Mirko Luoni, Cinzia Cancellieri, Alessandro Sessa, Marco Bacigaluppi, Stefano Taverna, Letizia Leocani, José L Lanciego, Vania Broccoli
The lack of technology for direct global-scale targeting of the adult mouse nervous system has hindered research on brain processing and dysfunctions. Currently, gene transfer is normally achieved by intraparenchymal viral injections, but these injections target a restricted brain area. Herein, we demonstrated that intravenous delivery of adeno-associated virus (AAV)-PHP.B viral particles permeated and diffused throughout the neural parenchyma, targeting both the central and the peripheral nervous system in a global pattern...
December 6, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/28790886/molecular-bases-of-brain-preconditioning
#20
Oleg G Deryagin, Svetlana A Gavrilova, Khalil L Gainutdinov, Anna V Golubeva, Vyatcheslav V Andrianov, Guzel G Yafarova, Sergey V Buravkov, Vladimir B Koshelev
Preconditioning of the brain induces tolerance to the damaging effects of ischemia and prevents cell death in ischemic penumbra. The development of this phenomenon is mediated by mitochondrial adenosine triphosphate-sensitive potassium ([Formula: see text]) channels and nitric oxide signaling (NO). The aim of this study was to investigate the dynamics of molecular changes in mitochondria after ischemic preconditioning (IP) and the effect of pharmacological preconditioning (PhP) with the [Formula: see text]-channels opener diazoxide on NO levels after ischemic stroke in rats...
2017: Frontiers in Neuroscience
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