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https://www.readbyqxmd.com/read/29029246/the-epidermal-growth-factor-network-role-in-oocyte-growth-maturation-and-developmental-competence
#1
Dulama Richani, Robert B Gilchrist
BACKGROUND: The LH surge induces great physiological changes within the preovulatory follicle, which culminate in the ovulation of a mature oocyte that is capable of supporting embryo and foetal development. However, unlike mural granulosa cells, the oocyte and its surrounding cumulus cells are not directly responsive to LH, indicating that the LH signal is mediated by secondary factors produced by the granulosa cells. The mechanisms by which the oocyte senses the ovulatory LH signal and hence prepares for ovulation has been a subject of considerable controversy for the past four decades...
September 20, 2017: Human Reproduction Update
https://www.readbyqxmd.com/read/29029022/functional-characterization-of-microrna-27a-3p-expression-in-human-polycystic-ovary-syndrome
#2
Mingming Wang, Jing Sun, Bo Xu, Marcin Chrusciel, Jun Gao, Maciei Bazert, Joanna Stelmaszewska, Yunyun Xu, Hongwen Zhang, Leszek Pawelczyk, Fei Sun, Nafis Rahman, Slawomir Wolczynski, Suk Ying Tsang, Xiangdong Li
The goal of this study was to characterize the function of microRNA-27a-3p (miR-27a-3p) in polycystic ovary syndrome (PCOS). MiR-27a-3p expression was analyzed in excised granulosa cells (GCs) from 21 PCOS and 12 normal patients undergoing IVF cycle treatments, and in 17 non-treated cuneiform ovarian resection PCOS samples and 13 control ovarian samples-from non-PCOS patients. We found that the expression of miR-27a-3p was significantly increased in both excised GCs and the ovaries of PCOS patients compared to the controls...
September 29, 2017: Endocrinology
https://www.readbyqxmd.com/read/29028570/bovine-ovarian-follicular-growth-and-development-correlate-with-lysophosphatidic-acid-expression
#3
Emilia Sinderewicz, Katarzyna Grycmacher, Dorota Boruszewska, Ilona Kowalczyk-Zięba, Joanna Staszkiewicz, Tomasz Ślężak, Izabela Woclawek-Potocka
The basis of successful reproduction is proper ovarian follicular growth and development. In addition to prostaglandins and vascular endothelial growth factor, a number of novel factors are suggested as important regulators of follicular growth and development: PGES, TFG, CD36, RABGAP1, DBI and BTC. This study focuses on examining the expression of these factors in granulosa and thecal cells that originate from different ovarian follicle types and their link with the expression of lysophosphatidic acid (LPA), known local regulator of reproductive functions in the cow...
September 25, 2017: Theriogenology
https://www.readbyqxmd.com/read/29026721/expression-of-the-genes-for-peroxisome-proliferator-activated-receptor-%C3%AE-cyclooxygenase-2-and-proinflammatory-cytokines-in-granulosa-cells-from-women-with-polycystic-ovary-syndrome
#4
Joong Yeup Lee, Jin Cheol Tae, Chung Hyon Kim, Doyeong Hwang, Ki Chul Kim, Chang Suk Suh, Seok Hyun Kim
OBJECTIVE: To identify differences in the expression of the genes for peroxisome proliferator-activated receptor (PPAR)-γ, cyclooxygenase (COX)-2, and the proinflammatory cytokines interleukin (IL)-6 and tumor necrosis factor (TNF)-α in granulosa cells (GCs) from polycystic ovary syndrome (PCOS) patients and controls undergoing controlled ovarian stimulation. METHODS: Nine patients with PCOS and six controls were enrolled in this study. On the day of oocyte retrieval, GCs were collected from pooled follicular fluid...
September 2017: Clinical and Experimental Reproductive Medicine
https://www.readbyqxmd.com/read/29025092/heat-stress-induces-autophagy-in-pig-ovaries-during-follicular-development
#5
Benjamin J Hale, Candice L Hager, Jacob T Seibert, Joshua T Selsby, Lance H Baumgard, Aileen F Keating, Jason W Ross
Hyperthermia or heat stress (HS) occurs when heat dissipation mechanisms are overwhelmed by external and internal heat production. Hyperthermia negatively affects reproduction and potentially compromises oocyte integrity and reduces developmental competence of ensuing embryos. Autophagy is the process by which cells recycle energy through the reutilization of cellular components and is activated by a variety of stressors. Study objectives were to characterize autophagy-related proteins in the ovary following cyclical HS during the follicular phase...
August 28, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29025042/stage-specific-follicular-extracellular-vesicle-uptake-and-regulation-of-bovine-granulosa-cell-proliferation
#6
Wei-Ting Hung, Raphatphorn Navakanitworakul, Tarique Khan, Pan Zhang John S Davis, Lynda K McGinnis, Lane K Christenson
Follicular fluid within ovarian antral follicles contains numerous factors, which influence the development of a healthy oocyte including nucleic acids, steroids, proteins, and extracellular vesicles (EVs). Current evidence indicates that follicular EVs promote changes in cellular gene expression and support cumulus-oocyte-complex expansion in vitro. In this study, we found EVs from different sized follicles differentially stimulate granulosa cell proliferation and this could be explained by both the differential contents associated, on or within the vesicles and by the preferential uptake of EVs dependent on follicle size from which they were isolated...
August 28, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29025011/significance-of-epidermal-growth-factor-receptor-signaling-for-acquisition-of-meiotic-and-developmental-competence-in-mammalian-oocytes
#7
Radek Prochazka, Milan Blaha, Lucie Nemcová
The surge in luteinizing hormone (LH) in preovulatory ovarian follicles triggers the resumption of oocyte meiosis accompanied by expansion of surrounding cumulus cells and ovulation of cumulus-oocyte complexes (COCs) into the oviduct. Over the last 15 years, substantial progress has been made in elucidating the key pathways by which the LH signal spreads within the preovulatory follicle and in identifying the molecules responsible for maintaining oocyte arrest and meiosis resumption. It is now clear that the adenylcyclase-mediated rise in intracellular cAMP leads to activation of the epidermal growth factor receptor (EGFR) network in granulosa and cumulus cells...
August 31, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29024988/low-dose-metronomic-delivery-of-cyclophosphamide-is-less-detrimental-to-granulosa-cell-viability-and-ovarian-function-than-maximum-tolerated-dose-delivery-in-the-mouse
#8
Jacqueline Dynes, Kata Osz, Allyssa Hooper, Jim Petrik
Chemotherapy can cause early menopause or infertility in women and have a profound negative impact on the quality of life of young female cancer survivors. Various factors are known to influence the risk of chemotherapy-induced ovarian failure, including the drug dose and treatment duration; however, the scheduling of dose administration has not yet been evaluated as an independent risk factor. We hypothesized that Low-Dose Metronomic (LDM) chemotherapy scheduling would be less detrimental to ovarian function than the traditional Maximum-Tolerated Dose (MTD) strategy...
August 30, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29024968/iron-suppresses-ovarian-granulosa-cell-proliferation-and-arrests-cell-cycle-through-regulating-p38-mapk-p53-p21-pathway
#9
Mei-Jou Chen, Chia-Hong Chou, Chia-Tung Shun, Tsui-Lien Mao, Wen-Fen Wen, Chin-Der Chen, Shee-Uan Chen, Yu-Shih Yang, Hong-Nerng Ho
Iron is an essential nutrient that may exert toxic effects when it accumulates in tissues. Little is known regarding its effects on gonadal function. Both Fe2+ and Fe3+ could be released from iron deposition. We employed mouse non-luteinized granulosa cell for in vitro studies and human ovarian tissues for Prussian blue and immunohistochemical staining to identify the iron deposition and effect in vivo. After treatment with FeSO4-7H2O or FeCl3 in granulosa cell cultured with follicle stimulating hormone (FSH) for 48 hours, we found that Fe2+ significantly suppressed FSH-induced granulosa cell proliferation and arrested the cell cycle at the G2/M phase by cell proliferation assay and flow cytometry...
August 28, 2017: Biology of Reproduction
https://www.readbyqxmd.com/read/29024053/direct-effect-of-pholyphenol-rich-plants-rooibos-and-ginkgo-on-porcine-ovarian-cell-functions
#10
A Štochmaľová, A Kádasi, R Alexa, M Bauer, A H Harrath, A V Sirotkin
The polyphenol-rich plants rooibos and ginkgo are widely used in folk medicine and in preparation of foods and drinks, but their effect on reproduction has not been properly studied yet. The aim of our in vitro experiments was to examine the possible direct effect of rooibos and ginkgo on the basic ovarian cell functions-proliferation, apoptosis and release of hormones progesterone (P4) and leptin (L). Porcine ovarian granulosa cells were cultured in the presence of rooibos or ginkgo extract (0, 1, 10 and 100 μg/ml of medium)...
October 11, 2017: Journal of Animal Physiology and Animal Nutrition
https://www.readbyqxmd.com/read/29022861/molecular-characterization-prevalence-and-clinical-relevance-of-phodopus-sungorus-papillomavirus-type-1-psupv1-naturally-infecting-siberian-hamsters-phodopus-sungorus
#11
Boštjan J Kocjan, Lea Hošnjak, Joško Račnik, Marko Zadravec, Nejc Bakovnik, Blaž Cigler, Monique Ummelen, Anton H N Hopman, Nina Gale, Tanja Švara, Mitja Gombač, Mario Poljak
Phodopus sungorus papillomavirus type 1 (PsuPV1), naturally infecting Siberian hamsters (Phodopus sungorus) and clustering in the genus Pipapillomavirus (Pi-PV), is only the second PV type isolated from the subfamily of hamsters. In silico analysis of three independent complete viral genomes obtained from cervical adenocarcinoma, oral squamous cell carcinoma and normal oral mucosa revealed that PsuPV1 encodes characteristic viral proteins (E1, E2, E4, E6, E7, L1 and L2) with conserved functional domains and a highly conserved non-coding region...
October 12, 2017: Journal of General Virology
https://www.readbyqxmd.com/read/29018356/xiao-yao-san-a-chinese-medicine-formula-ameliorates-chronic-unpredictable-mild-stress-induced-polycystic-ovary-in-rat
#12
Hao-Yu Sun, Quan Li, Yu-Ying Liu, Xiao-Hong Wei, Chun-Shui Pan, Jing-Yu Fan, Jing-Yan Han
Chronic stress induces endocrine disturbance, which contributes to the development of polycystic ovary syndrome (PCOS), a condition that remains a challenge for clinicians to cope with. The present study investigated the effect of Xiao-Yao-San (XYS), a traditional Chinese medicine formula used for treatment of gynecological disease, on the chronic stress-induced polycystic ovary and its underlying mechanism. Female Sprague-Dwaley rats underwent a 3 weeks chronic unpredictable mild stress (CUMS) procedure to establish the PCOS model, followed by 4 weeks treatment with XYS (0...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/29017851/seasonal-ovarian-immunolocalization-of-neuropeptide-y-and-its-role-in-steriodogenesis-in-asian-catfish-clarias-batrachus
#13
Priyadarshini, Bechan Lal
The present study was undertaken to examine the cellular localization and potential steroidogenic role of neuropeptide Y (NPY) in the ovary of the freshwater catfish, Clarias batrachus. NPY-immunoreaction was observed in the follicular cells (granulosa and thecal cells) in the growing ovarian follicles, and the intensity of staining increased steadily from the initiation of follicular development until follicles were fully grown. Thereafter as follicles matured the stain intensity decreased. Positive correlations were found between NPY expression and the ovarian levels of 17β-estradiol, testosterone, and activities of 3β-hydroxysteroid dehydrogenase (3β-HSD) and 17β-hydroxysteroid dehydrogenase (17β-HSD) in the ovary...
October 7, 2017: General and Comparative Endocrinology
https://www.readbyqxmd.com/read/28994896/adult-granulosa-cell-tumor-of-the-testis-a-case-report
#14
Mohamed Amine Bani, Alia Zehani, Ines Chelly, Atayaoui Fethi, Slim Haouet, Nidhamedine Kchir
No abstract text is available yet for this article.
December 2016: La Tunisie Médicale
https://www.readbyqxmd.com/read/28992815/correction-to-expression-of-factors-involved-in-apoptosis-and-cell-survival-is-correlated-with-enzymes-synthesizing-lysophosphatidic-acid-and-its-receptors-in-granulosa-cells-originating-from-different-types-of-bovine-ovarian-follicles
#15
Emilia Sinderewicz, Katarzyna Grycmacher, Dorota Boruszewska, Ilona Kowalczyk-Zięba, Joanna Staszkiewicz, Tomasz Ślężak, Izabela Woclawek-Potocka
No abstract text is available yet for this article.
October 9, 2017: Reproductive Biology and Endocrinology: RB&E
https://www.readbyqxmd.com/read/28988114/regulatory-effects-of-the-ampk%C3%AE-sirt1-molecular-pathway-on-insulin-resistance-in-pcos-mice-an-in-vitro-and-in-vivo-study
#16
Xin Tao, Lei Chen, Lisi Cai, Shuqi Ge, Xuanying Deng
In order to preliminarily explore the correlation between the AMPKα-SIRT1 pathway and insulin resistance and reproductive function in PCOS mice and find the pathogenesis molecular mechanism and potential therapeutic target of PCOS, we carried out in vitro study of human granulosa KGN cells and in vivo study of PCOS mouse model which was constructed with DHEA, and AICAR and Compound C were applied. We have found that SIRT1 and AMPKα expression in KGN cells gradually decreased as DHEA concentration increased; Mice of the PCOS model were in an obvious status of IR (P < 0...
October 5, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28986745/nutritional-and-metabolic-stressors-on-ovine-oocyte-development-and-granulosa-cell-functions-in-vitro
#17
S Nandi, S K Tripathi, P S P Gupta, S Mondal
The present study was undertaken to study the effect of ammonia, urea, non-esterified fatty acid (NEFA), and β-hydroxybutyric acid (β-OHB) on oocyte development and granulosa cell (GC) growth parameter of ovine (Ovis aries). Ovine oocytes were matured in vitro in the presence of different concentration of ammonia, urea, NEFA, and β-OHB for 24 h, in vitro inseminated and evaluated for cleavage and blastocyst yield. Same concentrations of ammonia, urea, NEFA, and β-OHB were examined on growth parameters and hormone secretion activity of granulosa cells in vitro...
October 6, 2017: Cell Stress & Chaperones
https://www.readbyqxmd.com/read/28986734/antral-follicle-responsiveness-to-fsh-assessed-by-the-follicular-output-rate-fort-is-altered-in-hodgkin-s-lymphoma-when-compared-with-breast-cancer-candidates-for-fertility-preservation
#18
Charlotte Sonigo, Marjorie Comtet, Solene Duros, Christophe Sifer, Nathalie Sermondade, Michaël Grynberg
PURPOSE: Oocyte and/or embryo cryopreservation after controlled ovarian hyperstimulation (COH) represents the most established method for female fertility preservation (FP) before cancer treatment. Whether patients suffering from malignancies, candidates for FP, have a normal ovarian capacity to respond to stimulation is controversial. Reduced responsiveness of antral follicle to exogenous FSH might be at play. The percentage of antral follicles that successfully respond to FSH administration may be estimated by the follicular output rate (FORT), which presumably reflects the health of granulosa cells...
October 6, 2017: Journal of Assisted Reproduction and Genetics
https://www.readbyqxmd.com/read/28986302/activation-of-nrf2-might-reduce-oxidative-stress-in-human-granulosa-cells
#19
Nana Akino, Osamu Wada-Hiraike, Hiromi Terao, Harunori Honjoh, Wataru Isono, Houju Fu, Mana Hirano, Yuichiro Miyamoto, Michihiro Tanikawa, Miyuki Harada, Tetsuya Hirata, Yasushi Hirota, Kaori Koga, Katsutoshi Oda, Kei Kawana, Tomoyuki Fujii, Yutaka Osuga
Nuclear factor-E2-related factor 2 (Nrf2)/Kelch-like ECH-associated protein 1 (Keap1)-antioxidant response element (ARE) signaling pathway is one of the most important defense mechanisms against oxidative stress (OS). It is well documented that equilibration status of OS plays fundamental roles in human reproductive medicine, and the physiological role of Nrf2 in ovarian granulosa cells (GCs) has not been determined yet. Herein we aimed to study the function of Nrf2 in GCs. Human ovarian tissues were subjected to immunohistochemistry to localize Nrf2 and Keap1 and we detected the expression of Nrf2 and Keap1 in the human GCs...
October 4, 2017: Molecular and Cellular Endocrinology
https://www.readbyqxmd.com/read/28983616/abnormal-expression-levels-of-bmp15-smad1-are-associated-with-granulosa-cell-apoptosis-in-patients-with-polycystic-ovary-syndrome
#20
Xiangrong Cui, Xuan Jing, Xueqing Wu, Xingyu Bi, Junfen Liu, Zhijing Long, Xiuping Zhang, Dongdong Zhang, Hongxiang Jia, Dan Su, Kai Huo
Polycystic ovary syndrome (PCOS) is a common endocrine disorder that affects reproductive dysfunction and metabolism in women of childbearing age. An increasing number of studies have suggested that the bone morphogenetic protein 15 (BMP15) signalling pathway serves an important role in the pathogenesis of PCOS; however, the full mechanism remains unknown. The present study revealed that intrinsic follicular dysplasia may be associated with regulation disorders of ovarian granulosa cell apoptosis. Compared with the control group, body mass index, luteinising hormone and testosterone levels were significantly increased (P<0...
September 28, 2017: Molecular Medicine Reports
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