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https://www.readbyqxmd.com/read/29455258/effects-of-capsaicin-on-ovarian-granulosa-cell-proliferation-and-apoptosis
#1
Sabire Güler, Berrin Zik
Capsaicin is the pungent ingredient in red peppers. Due to the effects on the sensory nerve fibers, capsaicin has been used to treat pain and inflammation associated with a variety of diseases including rheumatoid arthritis and diabetic neuropathy, obesity, and cardiovascular and gastrointestinal conditions. Despite the extensive publications on different systems, the studies of the effects on the ovary are very limited. The present study was conducted to examine the possible proliferative and/or apoptotic effects of various doses of capsaicin on primarily derived granulosa cells...
February 17, 2018: Cell and Tissue Research
https://www.readbyqxmd.com/read/29450927/histological-and-immunohistochemical-evaluation-of-granulosa-cells-during-different-stages-of-folliculogenesis-in-bovine-ovaries
#2
App Teh, U Z Izzati, K Mori, N Fuke, T Hirai, G Kitahara, R Yamaguchi
Bovine granulosa cells (GC) vary in their morphological aspect during different stages of folliculogenesis. In this study, 10 morphologically normal bovine ovaries were collected to study the structural aspects of different stages of GC using intermediate filament protein antibodies including cytokeratin AE1/AE3 (AE1/AE3), vimentin, nectin-4 and desmin. Hormonal immunolocalization was assessed using the immunomarkers anti-Müllerian hormone (AMH) and inhibin alpha. In addition, tumour markers and proliferation markers using c-erbB-2 oncoprotein and proliferating cell nuclear antigen, respectively, were investigated...
February 15, 2018: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/29449679/tert-promoter-mutation-in-adult-granulosa-cell-tumor-of-the-ovary
#3
Jessica A Pilsworth, Dawn R Cochrane, Zhouchunyang Xia, Geraldine Aubert, Anniina E M Färkkilä, Hugo M Horlings, Satoshi Yanagida, Winnie Yang, Jamie L P Lim, Yi Kan Wang, Ali Bashashati, Jacqueline Keul, Adele Wong, Kevin Norris, Sara Y Brucker, Florin-Andrei Taran, Bernhard Krämer, Annette Staebler, Esther Oliva, Sohrab P Shah, Stefan Kommoss, Friedrich Kommoss, C Blake Gilks, Duncan M Baird, David G Huntsman
The telomerase reverse transcriptase (TERT) gene is highly expressed in stem cells and silenced upon differentiation. Cancer cells can attain immortality by activating TERT to maintain telomere length and telomerase activity, which is a crucial step of tumorigenesis. Two somatic mutations in the TERT promoter (C228T; C250T) have been identified as gain-of-function mutations that promote transcriptional activation of TERT in multiple cancers, such as melanoma and glioblastoma. A recent study investigating TERT promoter mutations in ovarian carcinomas found C228T and C250T mutations in 15...
February 15, 2018: Modern Pathology: An Official Journal of the United States and Canadian Academy of Pathology, Inc
https://www.readbyqxmd.com/read/29448973/camp-response-element-binding-protein-1-controls-porcine-ovarian-cell-proliferation-apoptosis-and-fsh-and-insulin-like-growth-factor-1-response
#4
A V Sirotkin, A Benčo, A Tandlmajerová, M Lauková, D Vašíček, J Laurinčik, J Kornhauser, S Alwasel, A H Harrath
The aim of the present study was to examine the role of cAMP response element-binding protein (CREB) and its phosphorylation in the regulation of ovarian cell proliferation and apoptosis, and of the response of proliferation and apoptosis to the upstream hormonal stimulators FSH and insulin-like growth factor (IGF) 1. In the first series of experiments, porcine ovarian granulosa cells, transfected or not with a gene construct encoding wild-type CREB1 (CREB1WT), were cultured with and without FSH (0, 1, 10 or 100ngmL-1)...
February 16, 2018: Reproduction, Fertility, and Development
https://www.readbyqxmd.com/read/29447380/sirtuins-in-gamete-biology-and-reproductive-physiology-emerging-roles-and-therapeutic-potential-in-female-and-male-infertility
#5
Carla Tatone, Giovanna Di Emidio, Arcangelo Barbonetti, Gaspare Carta, Alberto M Luciano, Stefano Falone, Fernanda Amicarelli
BACKGROUND: Sirtuins (SIRT1-7) are a family of NAD+-dependent deacetylases that catalyze post-translational modifications of proteins. Together, they respond to metabolic challenges, inflammatory signals or hypoxic/oxidative stress, and are associated with aging and longevity. The role of Sirtuins in the regulation of fertility emerged in 2003 when a defective reproductive phenotype was observed in SIRT1-null mice. Although studies on Sirtuins in reproductive biology have been increasing in the last years, a recent comprehensive update on this issue is still lacking...
February 13, 2018: Human Reproduction Update
https://www.readbyqxmd.com/read/29439093/dysregulated-genes-and-their-functional-pathways-in-luteinized-granulosa-cells-from-pcos-patients-after-cabergoline-treatment
#6
Hortensia Ferrero, Patricia Diaz-Gimeno, Patricia Sebastian-Leon, Amparo Faus, Raul Gómez, Antonio Pellicer
Polycystic ovarian syndrome (PCOS) is a common reproductive disorder frequently associated with a substantial risk factor for ovarian hyperstimulation syndrome (OHSS). Dopamine receptor 2 (D2) agonists, like Cabergoline (Cb2), have been used to reduce the OHSS risk. However, lutein granulosa cells (LGCs) from PCOS patients treated with Cb2 still show a deregulated dopaminergic tone (decreased D2 expression and low dopamine production) and increased vascularization compared to non-PCOS LGCs. Therefore, to understand the PCOS ovarian physiology, it is important to explore the mechanisms that underlie syndrome based on the therapeutic effects of Cb2...
February 9, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29438534/both-in-vivo-fsh-depletion-and-follicular-exposure-to-gonadotrophin-releasing-hormone-analogues-in-vitro-are-not-effective-to-prevent-follicular-depletion-during-chemotherapy-in-mice
#7
F Horicks, G Van Den Steen, C Gervy, H J Clarke, I Demeestere
STUDY QUESTION: Does fertility preservation using gonadotrophin-releasing hormone (GnRH) analogues during chemotherapy act through a direct effect on the ovary or through inhibition of FSH secretion? SUMMARY ANSWER: The absence of FSH in vivo and the direct exposition of ovarian follicles to GnRH analogues in vitro did not prevent chemotherapy-induced ovarian damage. WHAT IS KNOWN ALREADY: The potential mechanisms of action of GnRH analogues in protecting ovaries against chemotherapy damage remain poorly understood...
February 9, 2018: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29438034/c1qtnf3-in-the-murine-ovary-and-its-function-in-folliculogenesis
#8
Zhoufei Mao, Liuhong Yang, Xiaosheng Lu, Anni Tan, Yuxia Wang, Fei Ding, Luanjuan Xiao, Xufeng Qi, Yanhong Yu
C1q/tumor necrosis factor-related protein 3 (C1QTNF3) is a novel adipokine with modulating effects on metabolism, inflammation, and the cardiovascular system. C1QTNF3 expression levels in the sera and omental adipose tissues of women with PCOS are low compared to control subjects. However, the expression and function of C1QTNF3 in the ovary has not previously been examined. Here, we assessed the expression patterns of C1qtnf3 in the ovary and explored its role in folliculogenesis. The C1qtnf3 transcript abundance was higher in large follicles than in small follicles and was under the influence of gonadotropin...
February 1, 2018: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/29437881/curcumin-exerts-a-protective-effect-against-premature-ovarian-failure-in-mice
#9
Zhengjie Yan, Youjin Dai, Heling Fu, Yuan Zheng, Dan Bao, Yuan Yin, Qin Chen, Xiaowei Nie, Qingting Hao, Daorong Hou, Yugui Cui
This study was designed to investigate the protective effect of curcumin against D-galactose (D-gal)-induced premature ovarian failure (POF) in mice. A mouse POF model was induced by subcutaneous injection of D-gal (200 mg.kg-1.d-1) daily for 42 days. Mice in the curcumin group received both D-gal treatment and intraperitoneal injection of curcumin (100 mg.kg-1.d-1) for 42 days. Ovarian function, oxidative stress and apoptosis were evaluated. The P, E2 and SOD levels were higher, and the FSH, LH and MDA levels were significantly lower in the curcumin group than in the D-gal group...
February 7, 2018: Journal of Molecular Endocrinology
https://www.readbyqxmd.com/read/29435998/environmental-mixture-with-estrogenic-activity-increases-hsd3b1-expression-through-estrogen-receptors-in-immature-rat-granulosa-cells
#10
Dragana Samardzija Nenadov, Kristina Pogrmic-Majkic, Svetlana Fa, Bojana Stanic, Aleksandra Tubic, Nebojsa Andric
Humans are exposed not only to single endocrine disruptors, but also to chemical mixtures that can adversely affect their reproductive health. Steroidogenesis in reproductive tissues is emerging as the key target of endocrine disruptor action. Here, we analyzed the effect of environmental chemical mixtures with estrogenic activity on steroidogenic processes in immature rat granulosa cells and whether the observed steroidogenic effects were mediated through estrogen receptors. Extracts from untreated wastewater were prepared by solid-phase extraction and silica gel fractionation...
February 13, 2018: Journal of Applied Toxicology: JAT
https://www.readbyqxmd.com/read/29435926/how-to-explore-the-function-and-importance-of-micrornas-micrornas-expression-profile-and-their-target-pathway-prediction-in-bovine-ovarian-cells
#11
Anna E Zielak-Steciwko, John A Browne
Micro RNAs (miRNA) are integral components of genetic regulatory networks and act by binding to the transcripts of their corresponding target genes, leading to a decrease in protein production levels either by mRNA degradation or by translational repression. While the role of miRNAs is ubiquitous, they have a particular importance with regard to cell differentiation. The miRNA-target mRNA interaction has a significant impact on many signaling pathways and the cross-talk between them; playing a regulatory role in a variety of different physiological processes within the cells...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29435922/isolation-and-analysis-of-exosomal-micrornas-from-ovarian-follicular-fluid
#12
Juliano Da Silveira, Gabriella M Andrade, Felipe Perecin, Flávio Vieira Meireles, Quinton A Winger, Gerrit J Bouma
Mammalian ovarian follicular growth is characterized by development of a large fluid filled antrum that separates mural granulosa cells and cumulus cells. Extensive communication between the different cell types is necessary for maturation of a developmentally competent oocyte. Here, we describe an approach for the isolation of cell-secreted exosomes from ovarian follicular fluid, identification of small RNAs (i.e., microRNAs) in exosomes, labeling of exosomes, and examining cell uptake of exosomes by follicular cells...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29432996/lncrna-let-inhibits-cell-viability-migration-and-emt-while-induces-apoptosis-by-up-regulation-of-timp2-in-human-granulosa-like-tumor-cell-line-kgn
#13
Qingfang Han, Wenke Zhang, Jinlai Meng, Li Ma, Aihua Li
BACKGROUND: Polycystic ovary syndrome (PCOS) is a common endocrine disease characterized by hyperandrogenism, irregular menses, and polycystic ovaries. Several long non-coding RNAs (lncRNAs) are aberrantly expressed in PCOS patients; however, little is known about the effects of the lncRNA-low expression in tumor (lncRNA-LET) on PCOS. We aimed to explore the effects of lncRNA-LET on human granulosa-like tumor cell line, KGN. METHODS: Expression of lncRNA-LET in normal IOSE80 cells and granulosa cells was determined by qRT-PCR...
February 9, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29428848/molecular-and-endocrine-factors-involved-in-future-dominant-follicle-dynamics-during-the-induction-of-luteolysis-in-bos-indicus-cows
#14
I Y H Martinez, A P C Santos, M P Bottino, R E Orlandi, G Santos, L M S Simões, J C Souza, A M G Díaza, M Binelli, J N S Sales
The growth profiles of the future dominant follicle (DF) and subordinate follicle (SF) and the gene expression of the granulosa cells during luteolysis induction in Bos indicus cows were evaluated. Forty cows were synchronized with a progesterone and estradiol based protocol. After synchronization, cows with a corpus luteum (CL) were evaluated by ultrasonography every 12 h, beginning at eight days post ovulation. Cows identified with a follicle of at least 6.0 mm in diameter in the second wave were split into two groups (BD-before follicular deviation and AD-after follicular deviation...
February 2, 2018: Theriogenology
https://www.readbyqxmd.com/read/29428847/cryopreservation-of-domestic-cat-felis-catus-ovarian-tissue-comparison-of-two-vitrification-methods
#15
D C C Brito, S F S Domingues, A P R Rodrigues, C Maside, F O Lunardi, X Wu, J R Figueiredo, J C Pieczarka, R R Santos
We aimed to evaluate the effect of two vitrification methods on the morphology and functionality of vitrified feline preantral follicles. Feline ovarian tissue was vitrified with EG + trehalose combined or not with dimethyl sulphoxide (DMSO), using two different techniques (open or closed systems). Morphology, developmental capacity and mRNA expression of markers for follicle survival and quality were assessed before and after in vitro culture (IVC). Both vitrification and culture media were serum-free. Vitrification of feline ovarian tissue from five adult domestic cats was performed with EG + trehalose combined or not with DMSO...
January 31, 2018: Theriogenology
https://www.readbyqxmd.com/read/29428312/reproductive-and-metabolic-determinants-of-granulosa-cell-dysfunction-in-normal-weight-women-with-polycystic-ovary-syndrome
#16
Annie A Guedikian, Alexandria Y Lee, Tristan R Grogan, David H Abbott, Karla Largaespada, Gregorio D Chazenbalk, Daniel A Dumesic
OBJECTIVE: To determine the degree to which E2 hyperresponsiveness to FSH and antimüllerian hormone (AMH) overproduction in normal-weight women with polycystic ovary syndrome (PCOS) correlate with increased antral follicle number (AFN), hyperandrogenism, and/or metabolic dysfunction. DESIGN: Prospective cohort study. SETTING: Academic medical center. PATIENT(S): Seven normal-weight women with PCOS (1990 National Institutes of Health criteria) ages 20-34 years and 13 age- and body mass index- (BMI-; 18...
February 7, 2018: Fertility and Sterility
https://www.readbyqxmd.com/read/29427807/isolation-and-culture-of-chicken-growing-follicles-in-2-and-3-dimensional-models
#17
Dan Zhao, Imdad Hussain Leghari, Jian Li, Yuling Mi, Caiqiao Zhang
The isolation and culture of ovarian follicles is essential for the studies of follicular development and function. In contrast to the relative ease of culture for mammalian follicles, developing in vitro cultures of high viability for the much larger avian follicles has always proven to be more challenging. In this study, the growing follicles from domestic hens (Gallus domesticus) were isolated using enzymatic and mechanical methods and then investigated for the optimized conditions for culture. Assessments of viability and hormonal responsiveness were also considered...
February 2, 2018: Theriogenology
https://www.readbyqxmd.com/read/29425943/chlorinated-insecticides-toxaphene-and-endrin-affect-oxytocin-testosterone-oestradiol-and-prostaglandin-secretion-from-ovarian-and-uterine-cells-as-well-as-myometrial-contractions-in-cow-in%C3%A2-vitro
#18
Michael Hubert Wrobel, Jaroslaw Mlynarczuk
We examined the direct effects of toxaphene and endrin, chlorinated insecticides that are widespread in the environment, on myometrial contractions and on the secretion of hormones involved in regulating these contractions. Granulosa, luteal, endometrial and myometrial cells, and myometrial strips from non-pregnant cows were incubated with both insecticides at environmentally relevant doses. Toxaphene inhibited and endrin stimulated the secretion of testosterone and oestradiol from granulosa cells. Toxaphene also inhibited and endrin stimulated the expression of the mRNA encoding the precursor of oxytocin (OT), as well OT secretion in luteal cell cultures...
February 3, 2018: Chemosphere
https://www.readbyqxmd.com/read/29425314/trio-a-novel-bovine-high-fecundity-allele-iii-acquisition-of-dominance-and-ovulatory-capacity-at-a-smaller-follicle-size
#19
Alvaro García-Guerra, Aurea M O Canavessi, Pedro L J Monteiro Jr, Megan A Mezera, Roberto Sartori, Brian W Kirkpatrick, Milo C Wiltbank
The acquisition of dominance and ovulatory capacity was evaluated in follicles from cows that were carriers or half-sibling non-carriers of the Trio allele. Follicle size at acquisition of follicular dominance was determined by evaluating whether follicles ovulate after GnRH challenge (ovulatory capacity-experiment 1) and by determination of intra-follicular concentrations of estradiol and free IGF1 and relative mRNA expression of CYP19A1, LHCGR, and PAPPA in granulosa cells from follicles of different sizes (experiment 2)...
February 7, 2018: Biology of Reproduction
https://www.readbyqxmd.com/read/29422312/altered-expression-of-anti-m%C3%A3-llerian-hormone-during-the-early-stage-of-bovine-persistent-ovarian-follicles
#20
P U Díaz, F Rey, N C Gareis, U S Notaro, V Matiller, E M Belotti, A F Stassi, N R Salvetti, H H Ortega
Anti-Müllerian hormone (AMH) is a homodimeric glycoprotein expressed exclusively in the gonads. This hormone is an important regulator of the early growth of follicles through inhibitory effects on the recruitment of primordial follicles into the pool of growing follicles and on granulosa cell proliferation. Cystic ovarian disease (COD) is an important disorder affecting the fertility of dairy cattle. In the present study, we evaluated the expression of AMH in granulosa cells and AMH secretion into follicular fluid in pre-ovulatory follicles from control cows, animals with spontaneously arising COD and during the development of the disease, at 5, 10 and 15 days of follicular persistence...
January 2018: Journal of Comparative Pathology
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