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https://www.readbyqxmd.com/read/30103825/changes-in-malaria-vector-bionomics-and-transmission-patterns-in-the-equatorial-forest-region-of-cameroon-between-2000-and-2017
#1
Roland Bamou, Lili Ranaise Mbakop, Edmond Kopya, Cyrille Ndo, Parfait Awono-Ambene, Timoleon Tchuinkam, Martin Kibet Rono, Joseph Mwangangi, Christophe Antonio-Nkondjio
BACKGROUND: Increased use of long-lasting insecticidal nets (LLINs) over the last decade has considerably improved the control of malaria in sub-Saharan Africa. However, there is still a paucity of data on the influence of LLIN use and other factors on mosquito bionomics in different epidemiological foci. The objective of this study was to provide updated data on the evolution of vector bionomics and malaria transmission patterns in the equatorial forest region of Cameroon over the period 2000-2017, during which LLIN coverage has increased substantially...
August 13, 2018: Parasites & Vectors
https://www.readbyqxmd.com/read/30086757/imported-and-autochthonous-malaria-in-west-saudi-arabia-results-from-a-reference-hospital
#2
Rasha Hassan Soliman, Patricia Garcia-Aranda, Sherine Mohamed Elzagawy, Boshra El-Sayed Hussein, Wael Wahid Mayah, Alexandra Martin Ramirez, Thuy-Huong Ta-Tang, José Miguel Rubio
BACKGROUND: The Kingdom of Saudi Arabia is seeking malaria eradication. Malaria transmission has been very low over the last few years. Discovered cases of Plasmodium falciparum infection are assigned a treatment protocol of artemisinin-based combination therapy, which consists of artesunate in addition to sulfadoxine-pyrimethamine rather than the traditional chloroquine, which has high resistance rates worldwide. This study aims to investigate the presence of different gene mutations concerning anti-malarial drug resistance (pfdhfr, pfdhps, pfmdr1, pfcrt, pfcytb, pfketch13) to identify whether drug-resistant alleles are present in this area of the Kingdom and whether the country's treatment protocol is still suitable for Plasmodium bearing a resistance mutation...
August 7, 2018: Malaria Journal
https://www.readbyqxmd.com/read/30080904/the-usefulness-of-c-reactive-protein-in-predicting-malaria-parasitemia-in-a-sub-saharan-african-region
#3
Bismark Osei Sarfo, Andreas Hahn, Norbert Georg Schwarz, Anna Jaeger, Nimako Sarpong, Florian Marks, Yaw Adu-Sarkodie, Thalea Tamminga, Juergen May
BACKGROUND: Malaria remains a leading cause of childhood mortality in sub-Saharan Africa. Identifying patients who are at risk for severe manifestations at presentation still remains challenging. This study examines whether a semi-quantitative test on C-Reactive Protein (CRP) could be useful for rapidly predicting the presence or absence of malarial parasitemia in febrile children. METHOD: Data were collected from children with fever or a history of fever at the Agogo Presbyterian Hospital in the Ashanti Region of Ghana...
2018: PloS One
https://www.readbyqxmd.com/read/30066720/serological-and-molecular-techniques-applied-for-identification-of-plasmodium-spp-in-blood-samples-from-nonhuman-primates
#4
Mayra Araguaia Pereira Figueiredo, Silvia Maria Di Santi, Wilson Gómez Manrique, Marcos Rogério André, Rosangela Zacarias Machado
The aim of this study was to identify Plasmodium spp. in blood samples from nonhuman primates (NHPs) in the state of Maranhão, using classical and alternative techniques for examination of human malaria. A total of 161 blood samples from NHPs were analyzed: 141 from captive animals at a Wildlife Screening Center (CETAS) and 20 from free-living animals in a private reserve. The techniques used were microscopy, rapid diagnostic test (RDT), Indirect fluorescent antibody test (IFAT) and molecular techniques (semi-nested PCR, quantitative real-time PCR and LAMP)...
July 30, 2018: Revista Brasileira de Parasitologia Veterinária, Brazilian Journal of Veterinary Parasitology
https://www.readbyqxmd.com/read/30062988/who-stays-and-who-goes-predictors-of-admission-among-patients-presenting-with-febrile-illness-and-a-positive-malaria-rapid-diagnostic-test-in-a-rural-ugandan-health-center
#5
Jonathan L Chang, Raquel Reyes, Michael Matte, Moses Ntaro, Edgar Mulogo, Matthew O Wiens, Steven R Meshnick, Mark J Siedner, Ross Boyce
Not much is known about clinical decision-making in rural, low-resource settings regarding fever, a common reason for presentation to care. In this prospective cohort study of patients presenting with febrile illness to a rural Ugandan health center, we examined demographic and clinical factors predictive of an initial disposition of inpatient admission after clinical evaluation, but before laboratory testing. We then assessed the association of laboratory results and system factors with a change between initial and final disposition plans...
July 30, 2018: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/30061051/safety-and-immunogenicity-of-pfs25h-epa-alhydrogel-a-transmission-blocking-vaccine-against-plasmodium-falciparum-a-randomised-double-blind-comparator-controlled-dose-escalation-study-in-healthy-malian-adults
#6
Issaka Sagara, Sara A Healy, Mahamadoun H Assadou, Erin E Gabriel, Mamady Kone, Kourane Sissoko, Intimbeye Tembine, Merepen A Guindo, M'Bouye Doucoure, Karamoko Niaré, Amagana Dolo, Kelly M Rausch, David L Narum, David L Jones, Nicholas J MacDonald, Daming Zhu, Rathy Mohan, Olga Muratova, Ibrahima Baber, Mamadou B Coulibaly, Michael P Fay, Charles Anderson, Yimin Wu, Sekou F Traore, Ogobara K Doumbo, Patrick E Duffy
BACKGROUND: Pfs25H-EPA is a protein-protein conjugate transmission-blocking vaccine against Plasmodium falciparum that is safe and induces functional antibodies in malaria-naive individuals. In this field trial, we assessed Pfs25H-EPA/Alhydrogel for safety and functional immunogenicity in Malian adults. METHODS: This double-blind, randomised, comparator-controlled, dose-escalation trial in Bancoumana, Mali, was done in two staggered phases, an initial pilot safety assessment and a subsequent main phase...
July 27, 2018: Lancet Infectious Diseases
https://www.readbyqxmd.com/read/30057630/low-frequency-of-asymptomatic-and-submicroscopic-plasmodial-infections-in-urab%C3%A3-region-in-colombia
#7
Carolina Rodríguez Vásquez, Sebastián Barrera Escobar, Alberto Tobón-Castaño
Background: A screening for malaria parasites was conducted with asymptomatic residents in Colombia. Methods: A descriptive study was carried out in December 2012 in four municipalities of Urabá region in Colombia. A convenience sample of 400 subjects was selected. Participants responded to a survey regarding epidemiological data and blood samples were taken from capillary blood obtained by finger prick for thick smear, rapid diagnostic test (RDT), and polymerase chain reaction (PCR)...
2018: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/30053187/a-diagnostic-performance-evaluation-of-rapid-diagnostic-tests-and-microscopy-for-malaria-diagnosis-using-nested-polymerase-chain-reaction-as-reference-standard-in-a-tertiary-hospital-in-jos-nigeria
#8
Okokon I Ita, Akaninyene A Otu, Kenneth Onyedibe, Anthony A Iwuafor, Edmund Banwat, Daniel Z Egah
Background: Rapid diagnostic tests are frequently used in healthcare settings across Nigeria for diagnosis of Plasmodium falciparum malaria, which is the commonest form of malaria in the country. In this study, the performance of a rapid diagnostic test (RDT) was compared with expert microscopy using the polymerase chain reaction (PCR) as the reference standard in a tertiary hospital in Jos, Nigeria. Methods: This study was a prospective, cross-sectional, hospital-based study...
July 25, 2018: Transactions of the Royal Society of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/30043723/retrospective-clinical-case-series-study-in-2017-identifies-plasmodium-knowlesi-as-most-frequent-plasmodium-species-in-returning-travellers-from-thailand-to-germany
#9
Guenter Froeschl, Hans Dieter Nothdurft, Frank von Sonnenburg, Gisela Bretzel, Roman Polanetz, Inge Kroidl, Michael Seilmaier, Hans Martin Orth, Sabine Jordan, Peter Kremsner, Sabine Vygen-Bonnet, Michael Pritsch, Michael Hoelscher, Camilla Rothe
Febrile illnesses are common in travellers returning from south-east Asia. However, malaria is a rare diagnosis in this population. A series of Plasmodium knowlesi infections was noted in German travellers returning from Thailand since 2012. Infectious disease and tropical medicine facilities registered by the German Society for Tropical Medicine and International Health were contacted in March 2017, and asked to report previous P. knowlesi cases. In addition, surveillance data from the Robert Koch-Institute were analysed...
July 2018: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/30019556/-epidemic-situation-of-imported-malaria-and-diagnostic-capabilities-of-medical-institutions-in-wuhan-city-from-2008-to-2017
#10
Wu Kai, Chen Fang, Xu Ming-Xing, Yang Yan
OBJECTIVE: To understand the epidemiological characteristics of imported malaria and the control and diagnostic capacities of the medical institutions in Wuhan City, so as to offer the evidence for formulating the surveillance and control strategies. METHODS: From 2008 to 2017, the epidemiological data of imported malaria were collected. The information including gender, age, distribution, vocational background, positive rate of fever patients, and time of final diagnosis was analyzed with the descriptive statistic method...
May 25, 2018: Zhongguo Xue Xi Chong Bing Fang Zhi za Zhi, Chinese Journal of Schistosomiasis Control
https://www.readbyqxmd.com/read/30016316/improving-rational-use-of-acts-through-diagnosis-dependent-subsidies-evidence-from-a-cluster-randomized-controlled-trial-in-western-kenya
#11
Wendy Prudhomme O'Meara, Diana Menya, Jeremiah Laktabai, Alyssa Platt, Indrani Saran, Elisa Maffioli, Joseph Kipkoech, Manoj Mohanan, Elizabeth L Turner
BACKGROUND: More than half of artemisinin combination therapies (ACTs) consumed globally are dispensed in the retail sector, where diagnostic testing is uncommon, leading to overconsumption and poor targeting. In many malaria-endemic countries, ACTs sold over the counter are available at heavily subsidized prices, further contributing to their misuse. Inappropriate use of ACTs can have serious implications for the spread of drug resistance and leads to poor outcomes for nonmalaria patients treated with incorrect drugs...
July 2018: PLoS Medicine
https://www.readbyqxmd.com/read/30012214/point-of-care-umbilical-arterial-lactate-and-newborn-outcomes-in-a-low-resource-setting-cohort-study
#12
George Kassim Chilinda, Luis Aaron Gadama, William Stones
OBJECTIVE: Birth asphyxia contributes substantially to the burden of intrapartum stillbirth and neonatal mortality in resource limited countries. We investigated clinical correlates and neonatal outcomes of lactate analysis of umbilical arterial cord blood in a large referral maternity unit in Malawi using a point-of-care test (Lactate Xpress, Nova Biomedical, Runcorn, UK) and examined maternal and neonatal characteristics and outcomes. RESULTS: There were 389 live births and 12 intrapartum stillbirths during the study...
July 16, 2018: BMC Research Notes
https://www.readbyqxmd.com/read/30011274/study-on-causes-of-fever-in-primary-healthcare-center-uncovers-pathogens-of-public-health-concern-in-madagascar
#13
Julia Guillebaud, Barivola Bernardson, Tsiry Hasina Randriambolamanantsoa, Laurence Randrianasolo, Jane Léa Randriamampionona, Cesare Augusto Marino, Voahangy Rasolofo, Milijaona Randrianarivelojosia, Ines Vigan-Womas, Voula Stivaktas, Marietjie Venter, Patrice Piola, Jean-Michel Héraud
BACKGROUND: The increasing use of malaria diagnostic tests reveals a growing proportion of patients with fever but no malaria. Clinicians and health care workers in low-income countries have few tests to diagnose causes of fever other than malaria although several diseases share common symptoms. We propose here to assess etiologies of fever in Madagascar to ultimately improve management of febrile cases. METHODOLOGY: Consenting febrile outpatients aged 6 months and older were recruited in 21 selected sentinel sites throughout Madagascar from April 2014 to September 2015...
July 2018: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/30005616/diagnostic-accuracy-of-loop-mediated-isothermal-amplification-lamp-for-screening-malaria-in-peripheral-and-placental-blood-samples-from-pregnant-women-in-colombia
#14
Ana María Vásquez, Lina Zuluaga, Alberto Tobón, Maritza Posada, Gabriel Vélez, Iveth J González, Ana Campillo, Xavier Ding
BACKGROUND: Pregnant women frequently show low-density Plasmodium infections that require more sensitive methods for accurate diagnosis and early treatment of malaria. This is particularly relevant in low-malaria transmission areas, where intermittent preventive treatment is not recommended. Molecular methods, such as polymerase chain reaction (PCR) are highly sensitive, but require sophisticated equipment and advanced training. Instead, loop mediated isothermal amplification (LAMP) provides an opportunity for molecular detection of malaria infections in remote endemic areas, outside a reference laboratory...
July 13, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29996831/malaria-and-the-last-parasite-how-can-technology-help
#15
REVIEW
Ngoc Minh Pham, Walter Karlen, Hans-Peter Beck, Emmanuel Delamarche
Malaria, together with HIV/AIDS, tuberculosis and hepatitis are the four most deadly infectious diseases globally. Progress in eliminating malaria has saved millions of lives, but also creates new challenges in detecting the 'last parasite'. Effective and accurate detection of malaria infections, both in symptomatic and asymptomatic individuals are needed. In this review, the current progress in developing new diagnostic tools to fight malaria is presented. An ideal rapid test for malaria elimination is envisioned with examples to demonstrate how innovative technologies can assist the global defeat against this disease...
July 11, 2018: Malaria Journal
https://www.readbyqxmd.com/read/29996301/-current-status-of-malaria-rapid-diagnostic-testing-strips-application-in-primary-level-healthcare-providers-in-jiangsu-province
#16
J J Jin, W M Wang, G D Zhu, H Y Zhou, J Cao, J Y Huang
Objective: To investigate the current status of malaria rapid diagnostic test (RDT) strips application and malaria laboratory technicians' evaluation about them at primary healthcare provider level in Jiangsu Province. Methods: From November to December 2016, 878 medical institutions and 118 CDCs of city, county and township/community level in Jiangsu Province were selected as study samples using stratified random sampling method. Self-designed questionnaire was distributed to investigate the institution's malaria work task, RDT strips application and evaluation status in 2015...
July 6, 2018: Zhonghua Yu Fang Yi Xue za Zhi [Chinese Journal of Preventive Medicine]
https://www.readbyqxmd.com/read/29995953/field-evaluation-of-a-real-time-loop-mediated-isothermal-amplification-assay-realamp-for-malaria-diagnosis-in-cruzeiro-do-sul-acre-brazil
#17
Giselle Maria Rachid Viana, Luciana Silva-Flannery, Danielle Regina Lima Barbosa, Naomi Lucchi, Suiane Costa Negreiros do Valle, Samela Farias, Nayara Barbalho, Paola Marchesini, Juliana Chedid Nogaredi Rossi, Venkatachalam Udhayakumar, Marinete Marins Póvoa, Alexandre Macedo de Oliveira
Conventional molecular methods, such as nested polymerase chain reaction (PCR), are very sensitive for detection of malaria parasites, but require advanced laboratory equipment and trained personnel. Real-time loop-mediated isothermal amplification (RealAmp), a loop-mediated isothermal amplification-based molecular tool (LAMP), facilitates rapid target amplification at a single temperature setting, reducing the need for sophisticated equipment. We evaluated the performance of a field-adapted RealAmp assay for malaria diagnosis in Cruzeiro do Sul, Acre State, Brazil, a remote area in Brazil with limited laboratory capabilities...
2018: PloS One
https://www.readbyqxmd.com/read/29988323/-plasmodium-falciparum-malaria-cases-detected-for-prompt-treatment-by-rapid-diagnostic-tests-in-the-ho-teaching-hospital-of-the-volta-region-of-ghana
#18
Bismarck Dinko, Eric Amakpa, Margaret Kweku, Paul Amoah, John Tampuori, Martin Adjuik, Gordon A Awandare, Kirk W Deitsch
Background: Prompt diagnosis and effective treatment of malaria cases with efficacious drugs is an important strategy in the management and control of malaria in endemic populations. As part of a study investigating the factors modulating the development of Plasmodium falciparum gametocytes in the human host, we assessed the rate of RDT positivity of patients in different departments of the Ho Teaching Hospital and the relation with age and anaemia. Materials and methods: Eight-hundred and ten individuals attending clinic at various departments within the Ho Teaching Hospital were screened for malaria antigenaemia using RDT as a point-of-entry investigation...
August 2018: Parasite Epidemiology and Control
https://www.readbyqxmd.com/read/29988270/school-aged-children-based-seasonal-malaria-chemoprevention-using-artesunate-amodiaquine-in-mali
#19
Mahamadou A Thera, Abdoulaye K Kone, Bourama Tangara, Elizabeth Diarra, Sirama Niare, Abdramane Dembele, Mahamadou S Sissoko, Ogobara K Doumbo
Introduction: Malaria is still a public health problem in Africa. Seasonal Malaria Chemoprevention (SMC) is an efficient control strategy recommended by WHO that targets children under five year old living in areas of seasonal malaria transmission. SMC uses the combination amodiaquine (AQ) - sulfadoxine-pyrimethamine (SP). However SP selects rapidly drug resistant parasites. And malaria burden may increase in older children where SMC is implemented. We initiated a pilot study to assess an alternative approach to SMC in older children in Mali...
May 2018: Parasite Epidemiology and Control
https://www.readbyqxmd.com/read/29986725/evidence-for-histidine-rich-protein-2-immune-complex-formation-in-symptomatic-patients-in-southern-zambia
#20
Christine F Markwalter, Lwiindi Mudenda, Mindy Leelawong, Danielle W Kimmel, Armin Nourani, Saidon Mbambara, Philip E Thuma, David W Wright
BACKGROUND: Rapid diagnostic tests based on histidine-rich protein 2 (HRP2) detection are the primary tools used to detect Plasmodium falciparum malaria infections. Recent conflicting reports call into question whether α-HRP2 antibodies are present in human host circulation and if resulting immune complexes could interfere with HRP2 detection on malaria RDTs. This study sought to determine the prevalence of immune-complexed HRP2 in a low-transmission region of Southern Zambia. METHODS: An ELISA was used to quantify HRP2 in patient sample DBS extracts before and after heat-based immune complex dissociation...
July 9, 2018: Malaria Journal
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