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Yu Ding, Yamei Yan, Yujia Peng, Dan Chen, Jia Mi, Lu Lu, Qing Luo, Xiaoying Li, Xiaoxiong Zeng, Youlong Cao
In the present study, the digestion under simulated saliva, gastric and small intestinal conditions and in vitro fermentation by human gut microbiota of Lycium barbarum polysaccharides (LBPS) were investigated. As a result, LBPS contained carbohydrates, uronic acids, protein and polyphenols of 60.96 ± 5.58, 20.98 ± 2.49, 5.30 ± 0.12 and 1.04 ± 0.06%, respectively. Furthermore, LBPS was mainly consisted of glucuronic acid (5.12 mol%), galacturonic acid (12.40 mol%), glucose (2.15 mol%), galactose (39...
December 12, 2018: International Journal of Biological Macromolecules
Janike Ehret, Martina Zimmermann, Thomas Eichhorn, Aline Zimmer
Glycosylation is a key critical quality attribute for monoclonal antibodies and other recombinant proteins because of its impact on effector mechanisms and half-life. In this study, a variety of compounds were evaluated for their ability to modulate glycosylation profiles of recombinant monoclonal antibodies produced in Chinese hamster ovary cells. Compounds were supplemented into the cell culture feed of fed-batch experiments performed with a CHO K1 and a CHO DG44 cell line expressing a recombinant IgG1. Experiments were performed in spin tubes or the ambr® 15 controlled bioreactor system, and the impact of the compounds at various concentrations was determined by monitoring the glycosylation profile of the IgG and cell culture parameters such as viable cell density, viability and titer...
December 15, 2018: Biotechnology and Bioengineering
Zachary D Moye, Courtney M Gormley, Mary E Davey
The asaccharolytic anaerobe Porphyromonas gingivalis metabolizes proteins it encounters in the periodontal pocket, including host-derived glycoproteins such as mucins and immunoglobulins. Often, these proteins are protected by a diverse array of carbohydrates tethered to the polypeptide chain via glycolytic bonds, and P. gingivalis produces enzymes capable of liberating these carbohydrates, exposing the proteinaceous core. In this study, we investigated the effect of individual monosaccharides, including galactose, L-fucose, mannose and glucose, on the growth and physiology of P...
December 14, 2018: Applied and Environmental Microbiology
Samaila Musa Chiroma, Mohamad Taufik Hidayat Baharuldin, Che Norma Mat Taib, Zulkhairi Amom, Saravanan Jagadeesan, Mohd Ilham Adenan, Mohamad Aris Mohd Moklas
BACKGROUND: Alzheimer's disease (AD) is a neurodegenerative disorder and the commonest cause of dementia among the aged people. D -galactose (D-gal) is a senescence agent, while aluminium is a known neurotoxin linked to pathogenesis of AD. The combined administration of rats with d-gal and aluminium chloride (AlCl3 ) is considered to be an easy and a cheap method to obtain an animal model of AD. The plant Centella asiatica (CA) is reported to exert neuroprotective effects both in vitro and in vivo...
January 2019: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Nicholas C Lazzara, Robert J Rosano, Purav P Vagadia, Matthew T Giovine, Mark W Bezpalko, Nicholas A Piro, Wm Scott Kassel, Walter J Boyko, Deanna Lynn Zubris, Kevin K Schrader, David E Wedge, Stephen O Duke, Robert M Giuliano
The phytotoxin diplopyrone is considered to be the main phytotoxin in a fungus that is responsible for cork oak decline. A carbohydrate-based synthesis of the enantiomer of the structure proposed for diplopyrone has been developed from 1,2:3,4-di-O-isopropylidene-α-D-galactopyranose, a commercially available derivative of D-galactose. Key steps in the synthesis are a highly stereoselective pyranose chain-extension based on methyltitanium, preparation of a vinyl glycoside via Isobe C-alkynylation-rearrangment/reduction, and RCM (ring-closing methathesis)-based pyranopyran construction...
December 14, 2018: Journal of Organic Chemistry
Scott A Cameron, Olga V Zubkova, Steven Toms, Richard H Furneaux, Phillip M Rendle
Morquio A syndrome is an autosomal mucopolysaccharide storage disorder that leads to accumulation of keratan sulfate. Diagnosis of this disease can be aided by measuring the levels of keratan sulfate in the urine. This requires the liquid chromatography tandem mass spectrometry (LCMS/MS) measurement of sulfated N-acetyl-d-lactosamines in the urine after cleavage of the keratan sulfate with keratanase II. Quantification requires isotopically-labelled internal standards. The synthesis of these 13 C6 -labelled standards from 13 C6 -galactose and N-acetylglucosamine is described...
November 22, 2018: Journal of Labelled Compounds & Radiopharmaceuticals
Zhi-Nan Zhu, Yan-Ru Li, Yu-Qian Li, Min Xiao, Ming-Xian Han, Mohammed A M Wadaan, Wael N Hozzein, Deng-Di An, Wen-Jun Li
Two bacterial strains, YZYP 306T and YZGP 509, were isolated from the halophyte Suaeda aralocaspica collected from the southern edge of the Gurbantunggut desert, north-west China. Cells were Gram-stain-positive, aerobic, non-motile, short rods. Strain YZYP 306T grew at 4-40 °C, while strain YZGP 509 grew at 4-42 °C, with optimum growth at 28 °C, and they both grew at pH 6.0-12.0 and 0-15 % (w/v) NaCl. Phylogenetic analyses of the 16S rRNA gene sequences placed the two strains within the genus Microbacterium with the highest similarities to Microbacterium indicum BBH6T (97...
December 14, 2018: International Journal of Systematic and Evolutionary Microbiology
Seyed Saeid Hosseini, Faramarz Khodaiyan, Milad Kazemi, Zahra Najari
In this work, a Box-Behnken design (BBD) with three variables (ultrasound power, irradiation time and pH) in three levels was applied for pectin extraction optimization. The optimization process showed that the maximum extraction yield was 28.07 ± 0.67% in ultrasound power of 150 W, irradiation time of 10 min and pH of 1.5 (as optimum conditions). In these conditions, ash, moisture and protein contents of SOPP were 1.89 ± 0.51, 8.81 ± 0.68 and 1.45 ± 0.23%, respectively. HPLC analysis indicated that 65...
December 10, 2018: International Journal of Biological Macromolecules
Rui-Qi Yang, Bing-Lin Zhang, Hai-Li Sun, Gao-Sen Zhang, Shi-Weng Li, Guang-Xiu Liu, Tuo Chen, Yun-Shi Li, Yong-Na Wu, Li-Zhe An, Wei Zhang, Xiu-Kun Wu
A Gram-stain-positive, aerobic, non-motile and mycolic-acid-containing strain, designated Y48T , was isolated from soil contaminated by crude oil located in the northern margin of the Qaidam Basin. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain Y48T belongs to the genus Nocardia and is closely related to N. cummidelens DSM 44490T (99.0 % similarity), N. soli DSM 44488T (99.0 %), N. lasii 3C-HV12T (98.9 %), N. salmonicida NBRC 13393T (98.6 %), N. ignorata NBRC 108230T (98...
December 13, 2018: International Journal of Systematic and Evolutionary Microbiology
Zhang-Gui Ding, Yang Ji, Min Yin, Yu-Rong Zhao, Yu-Zhou Feng, Wei-Xun Chunyu, Shu-Kun Tang
A novel halophilic actinobacterial strain, designated YIM 96934T , was isolated from a soil sample collected from the edge of a saline lake in Xinjiang, north-west China. The taxonomic position of the strain was investigated using a polyphasic approach. Cells of the strain were aerobic and Gram-stain-positive. On the basis of 16S rRNA gene sequence analysis, strain YIM 96934T was related most closely to type strains of the genus Phytoactinopolyspora, and shared highest sequence similarity with Phytoactinopolyspora endophytica EGI 60009T (94...
December 13, 2018: International Journal of Systematic and Evolutionary Microbiology
Lei Li, Yu-Han Gui, Qi-Hang Xu, Hou-Wen Lin, Yan-Hua Lu
A novel aerobic, spore-forming, marine actinomycete, designated strain LHW63015T , was isolated from a Craniella marine sponge collected in the South China Sea. The strain formed extensively branched substrate and aerial mycelia which carried long and crooked spore chains composed of ridged spores and spherical pseudosporangia. Strain LHW63015T contained meso-diaminopimelic acid as the diagnostic diamino acid. Glucose, ribose, mannose, galactose and madurose occured in whole-cell hydrolysates. The predominant polar lipids were hydroxyl-phosphatidylethanolamine, phosphoglycolipid and ninhydrin-positive phosphoglycolipid...
December 13, 2018: International Journal of Systematic and Evolutionary Microbiology
Goutam Banerjee, Atish Ray, Kazi Nurul Hasan
β-Galactosidase is a metal-activated enzyme, which breaks down the glucosidic bond of lactose and produces glucose and galactose. Among several commercial applications, preparation of lactose-free milk has gained special attention. The present objective is to demonstrate the activity kinetics of β-galactosidase purified from a non-pathogenic bacterium Arthrobacter oxydans SB. The enzyme was purified by DEAE-cellulose and Sephadex G-100 column chromatography. The purity of the protein was checked by high-performance liquid chromatography (HPLC)...
December 2018: Journal of Biosciences
Zhen Zhang, Hanlin Yang, Jingyu Yang, Jun Xie, Jiaoyan Xu, Chen Liu, Chunfu Wu
Oxidative stress and neuroinflammation are thought to be the two key early events during the process of mild cognitive impairment (MCI). Therefore, effective regulation of oxidative stress and neuroinflammation is an important aspect of preventing and improving MCI. We previously found that pseudoginsenoside-F11 (PF11), an ocotillol-type saponin, markedly reduced cognitive impairment in APP/PS1 mice and oAβ1-42 -injected mice. In the present study, we further evaluate the effect of PF11 on learning and memory dysfunction in d‑galactose (d‑gal)-treated mice model of MCI...
December 8, 2018: International Immunopharmacology
Kai Fan, Qunfeng Zhang, Meiya Liu, Lifeng Ma, Yuanzhi Shi, Jianyun Ruan
Tea flowering in late autumn competes for a large amount of nitrogen and carbohydrates, potentially undermines the storage of these resources in vegetative organs, and negatively influences the subsequent spring tea yield and quality. The mechanism underlying the re-allocation N and carbohydrate from source leaf to flower in tea plant has not been clearly understood. In this study, 15 N allocation, changes in metabolomics, and gene expression in flower buds, flowers, and adjacent leaves were characterized. Total N content of the adjacent leaves significantly decreased during flowering while such a decrease could be reversed by flower bud removal...
November 10, 2018: Journal of Plant Physiology
Lulu Chu, Licong Yang, Lezhen Lin, Jing Wei, Na Wang, Meng Xu, Gaoxiang Qiao, Guodong Zheng
The aim of this study is to investigate hypolipidemic and antioxidant effects of Pine needle polysaccharide (PNP) from Pinus massoniana in high-fat diet (HFD)-induced mice. PNP could significantly improve the serum lipid levels (total cholesterol, triacylglycerols, high-density lipoprotein cholesterol, low-density lipoprotein cholesterol), enhance the antioxidant enzymes levels (total antioxidant capability, superoxide dismutase, glutathione peroxidase, catalase), and decrease malondialdehyde (MDA) content in HFD-induced mice...
December 8, 2018: International Journal of Biological Macromolecules
Jia Song, Shiwen Qiu, Junwei Zhao, Chuanyu Han, Ying Wang, Xiujun Sun, Shanwen Jiang, Xiangjing Wang, Wensheng Xiang
An aerobic, non-motile, Gram-stain positive actinomycete, designated strain NEAU-YY211T , was isolated from the rhizosphere soil of wheat (Triticum aestivum L.) collected from Zhumadian, Henan Province, mid-eastern China, and characterised taxonomically using a polyphasic approach. Comparative analysis of the 16S rRNA gene sequence indicated that strain NEAU-YY211T belongs to the genus Pseudonocardia, showing high similarities with respect to Pseudonocardia ammonioxydans H9T (99.1%) and Pseudonocardia antitumoralis SCSIO 01299T (99...
December 7, 2018: Antonie Van Leeuwenhoek
Sonia Chamizo, Alessandra Adessi, Gianmarco Mugnai, Andrea Simiani, Roberto De Philippis
Inoculation of soils with cyanobacteria is proposed as a sustainable biotechnological technique for restoration of degraded areas in drylands due to the important role that cyanobacteria and their exopolysaccharides (EPS) play in the environment. So far, few studies have analyzed the macromolecular and chemical characteristics of the polysaccharidic matrix in induced cyanobacterial biocrusts and the scarce existing studies have mainly focused on sandy soil textures. However, the characteristics of the cyanobacterial polysaccharidic matrix may greatly depend on soil type...
December 8, 2018: Microbial Ecology
Feng Zhang, Weijiang Zheng, Yongqiang Xue, Wen Yao
Unabsorbed copper accumulates in the hindgut of pigs that consume high levels of dietary copper, which enhances the coselection of antibiotic-resistant bacteria and is considered detrimental to the environment and to porcine health. In our study, a combination of 16S rRNA pyrosequencing and nontargeted metabolomics was used to investigate the microbiome-metabolome responses to dietary copper levels in the hindgut of suckling piglets. The results showed that the dietary copper level affected the abundance of several Clostridia genera and that the relative abundance of butyrate-producing bacteria, such as Coprococcus, Roseburia, and Acidaminococcus, was reduced in the 300 mg kg-1 (high) Cu group...
December 7, 2018: Applied Microbiology and Biotechnology
Dan Wang, François Couderc, Chang Fu Tian, Wenjie Gu, Li Xue Liu, Verena Poinsot
The structural variation of symbiotic signals released by rhizobia determines the specificity of their interaction with legume plants. Previous studies showed that Sinorhizobium strains from different phylogenetic lineages had different symbiotic performance on certain cultivated soybeans. Whether they released similar or different symbiotic signals remained unclear. In this study, we compared their nod and exo gene clusters and made a detailed structural analysis of Nod factors and EPS by ESI-MS/MS and two dimensions NMR...
2018: Frontiers in Microbiology
Yujiro Higuchi, Hitomi Matsufuji, Kazuki Mori, Emiko Matsunaga, Kosuke Tashiro, Kaoru Takegawa
The genome sequence of the Bacillus sp. strain HMA207, the culture supernatant of which exhibited β-d-galactosidase activity to release pyruvylated galactose (PvGal), was examined to identify a PvGal-ase-encoding gene. We report here the result of whole-genome shotgun sequencing, which revealed putative PvGal-ase genes.
September 2018: Microbiology resource announcements
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