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https://www.readbyqxmd.com/read/28437177/periodontal-derived-mesenchymal-cell-sheets-promote-periodontal-regeneration-in-inflammatory-microenvironment
#1
Shujuan Guo, Jian Kang, Baohui Ji, Weihua Guo, Yi Ding, Yafei Wu, Weidong Tian
In this study, we investigated the periodontal regenerative potential of dental follicle cell (DFC) sheets and periodontal ligament cell (PDLC) sheets in the simulating inflammatory microenvironment of periodontitis, in order to confirm their regenerative potential for clinical application and explain the possible mechanism. The biological characteristics of DFC sheets and PDLC sheets were explored in lipopolysaccharid from Porphyromonas gingivalis (P. gingivalis LPS) induced inflammation microenvironment in vitro, then cell sheets were transplanted into canine periodontal defects with experimental periodontitis in situ for three months...
February 14, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28435478/disseminated-extrafacial-rosacea-with-papulonecrotic-lesions
#2
Toshio Demitsu, Rieko Tsukahara, Naoka Umemoto, Satoshi Nakamura, Kazutaka Nagashima, Tomoko Yamada, Maki Kakurai, Yoshiaki Tanaka, Akihiro Kakehashi, Toshiko Miyata
BACKGROUND: Rosacea is a common skin disease and predominantly affects on the face of middle-aged women. It exceptionally occurs on the extrafacial areas such as ear, neck, axilla, and upper extremities, and has been reported as disseminated rosacea. MAIN OBSERVATION: A 40-year-old Japanese female presented with one-month history of erythematous skin eruption with burning sensation on the face, neck, and upper limbs. Physical examination showed rosacea-like eruption on the face as well as multiple papules disseminated on the neck, forearms, and hands...
December 31, 2016: Journal of Dermatological Case Reports
https://www.readbyqxmd.com/read/28435200/mycotoxin-binders-potential-on-histological-of-ovary-mice-exposed-by-zearalenone
#3
Abdul Samik, Erma Safitri
AIM: This study was conducted to examine the potential of mycotoxin binder in ceasing zearalenone (ZEN) effect on mice reproduction. ZEN mycotoxin can induce reactive oxygen species that may cause damage and cell death. ZEN is estrogenic so that it may affect the reproductive organs failure. MATERIALS AND METHODS: Mycotoxin binder administration to female mice exposed to ZEN was aimed to count the number of primary follicles, secondary follicles, tertiary follicles, de Graaf's follicles, and the corpus luteum (CL)...
March 2017: Veterinary World
https://www.readbyqxmd.com/read/28435188/effect-of-kisspeptin-on-in-vitro-maturation-of-sheep-oocytes
#4
Priyanka Byri, Arunakumari Gangineni, K Ramachandra Reddy, K B P Raghavender
AIM: The aim of this study was to investigate the effect of kisspeptin (KP) on in vitro maturation (IVM) of sheep oocytes aspirated from the ovaries collected from slaughterhouse. MATERIALS AND METHODS: Two different experiments were conducted to investigate the effect of KP (5, 10 and 15 µg/ml) alone (experiment 1) or in combination with follicle-stimulating hormone (FSH), luteinizing hormone (LH), and Estradiol (E2) (experiment 2) on IVM of sheep oocytes. Tissue culture medium 199 supplemented with Gentamicin was used as control medium...
March 2017: Veterinary World
https://www.readbyqxmd.com/read/28435048/morella-serrata-lam-killick-stabilizes-biomembrane-and-rejuvenates-sexual-competence-in-male-wistar-rats
#5
S Sabiu, Aot Ashafa
ETHNOPHARMACOLOGICAL RELEVANCE: Morella serrata L. is an indigenous medicinal plant to South and southern Africa with folkloric applications as aphrodisiac, laxative, antimicrobial, anti-inflammatory, analgesic, anti-hypertensive, antitussive and antidiabetic agent. AIM OF THE STUDY: This study evaluated the membrane stabilization and aphrodisiac potentials of its aqueous root extract in male Wistar rats. MATERIALS AND METHODS: While the membrane stabilization of the extract was investigated against bovine erythrocytes (BE), the male rats for the aphrodisiac study were randomized into five groups with animals in group 1 given sterile placebo and served as control...
April 20, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28434825/rna-binding-proteins-in-human-oogenesis-balancing-differentiation-and-self-renewal-in-the-female-fetal-germline
#6
Roseanne Rosario, Andrew J Childs, Richard A Anderson
Primordial germ cells undergo three significant processes on their path to becoming primary oocytes: the initiation of meiosis, the formation and breakdown of germ cell nests, and the assembly of single oocytes into primordial follicles. However at the onset of meiosis, the germ cell becomes transcriptionally silenced. Consequently translational control of pre-stored mRNAs plays a central role in coordinating gene expression throughout the remainder of oogenesis; RNA binding proteins are key to this regulation...
April 18, 2017: Stem Cell Research
https://www.readbyqxmd.com/read/28434779/a-comparative-study-of-cutibacterium-propionibacterium-acnes-clones-from-acne-patients-and-healthy-controls
#7
H B Lomholt, C F P Scholz, H Brüggemann, H Tettelin, M Kilian
BACKGROUND: Cutibacterium (Propionibacterium) acnes is assumed to play an important role in the pathogenesis of acne. OBJECTIVES: To examine if clones with distinct virulence properties are associated with acne. METHODS: Multiple C. acnes isolates from follicles and surface skin of patients with moderate to severe acne and healthy controls were characterized by multilocus sequence typing. To determine if CC18 isolates from acne patients differ from those of controls in the possession of virulence genes or lack of genes conducive to a harmonious coexistence the full genomes of dominating CC18 follicular clones from six patients and five controls were sequenced...
April 18, 2017: Anaerobe
https://www.readbyqxmd.com/read/28434724/the-effect-of-presynchronization-with-prostaglandin-f2%C3%AE-and-gonadotropin-releasing-hormone-simultaneously-7-d-before-ovsynch-compared-with-presynch-10-ovsynch-on-luteal-function-and-first-service-pregnancies-per-artificial-insemination
#8
J P N Martins, M J T Acevedo, T O Cunha, C Piterini, J R Pursley
Pregnancy per artificial insemination (P/AI) following Ovsynch is optimized when cows ovulate to the first GnRH of Ovsynch. Fertility programs are designed to presynchronize cows to d 6 or 7 of the estrous cycle to increase the chances of ovulation of a first-wave dominant follicle to the first GnRH of Ovsynch. The hypothesis of this experiment was that simplification of a presynchronization program through the combination of PGF2α and GnRH on the same day, 7 d before Ovsynch, would allow for similar P/AI compared with Presynch-10...
April 20, 2017: Journal of Dairy Science
https://www.readbyqxmd.com/read/28434617/stem-cell-lineage-infidelity-drives-wound-repair-and-cancer
#9
Yejing Ge, Nicholas C Gomez, Rene C Adam, Maria Nikolova, Hanseul Yang, Akanksha Verma, Catherine Pei-Ju Lu, Lisa Polak, Shaopeng Yuan, Olivier Elemento, Elaine Fuchs
Tissue stem cells contribute to tissue regeneration and wound repair through cellular programs that can be hijacked by cancer cells. Here, we investigate such a phenomenon in skin, where during homeostasis, stem cells of the epidermis and hair follicle fuel their respective tissues. We find that breakdown of stem cell lineage confinement-granting privileges associated with both fates-is not only hallmark but also functional in cancer development. We show that lineage plasticity is critical in wound repair, where it operates transiently to redirect fates...
April 19, 2017: Cell
https://www.readbyqxmd.com/read/28433748/the-repertoire-of-epithelial-morphogenesis-on-display-progressive-elaboration-of-drosophila-egg-structure
#10
REVIEW
Juan Carlos Duhart, Travis T Parsons, Laurel A Raftery
Epithelial structures are foundational for tissue organization in all metazoans. Sheets of epithelial cells form lateral adhesive junctions and acquire apico-basal polarity perpendicular to the surface of the sheet. Genetic analyses in the insect model, Drosophila melanogaster, have greatly advanced our understanding of how epithelial organization is established, and how it is modulated during tissue morphogenesis. Major insights into collective cell migrations have come from analyses of morphogenetic movements within the adult follicular epithelium that cooperates with female germ cells to build a mature egg...
April 19, 2017: Mechanisms of Development
https://www.readbyqxmd.com/read/28433506/heifers-express-g-protein-coupled-receptor-61-in-anterior-pituitary-gonadotrophs-in-stage-dependent-manner
#11
Kiran Pandey, Onalenna Kereilwe, Vitaliano Borromeo, Hiroya Kadokawa
Gonadotropin-releasing hormone receptors (GnRHRs) colocalize with insulin and glucocorticoid receptors in lipid rafts of the gonadotroph plasma membrane, where they facilitate downstream signaling. We recently found that orphan G-protein-coupled receptor (GPR)61 is expressed in the anterior pituitary (AP) of heifers, leading us to speculate that GPR61 colocalizes with GnRHR in the plasma membrane of gonadotroph and is expressed at specific times of the reproductive cycle. To test this hypothesis, we examined the coexpression of GnRHR, GPR61, and either luteinizing hormone (LH) β subunit or follicle-stimulating hormone (FSH) β subunit in AP tissue and cultured AP cells by immunofluorescence microscopy...
April 4, 2017: Animal Reproduction Science
https://www.readbyqxmd.com/read/28433369/fertility-preservation-and-refreezing-of-transplanted-ovarian-tissue-a-potential-new-way-of-managing-patients-with-low-risk-of-malignant-cell-recurrence
#12
Stine Gry Kristensen, Veronica Giorgione, Peter Humaidan, Birgit Alsbjerg, Anne-Mette Bay Bjørn, Erik Ernst, Claus Yding Andersen
OBJECTIVE: To report the first successful refreezing of ovarian tissue recovered more than 3 years after transplantation in a woman previously treated for early-stage ovarian cancer. DESIGN: Evaluation of cryopreserved and grafted ovarian tissue. SETTING: University hospital. PATIENT(S): A 23-year-old woman diagnosed with stage 1C ovarian mucinous cystadenocarcinoma. INTERVENTION(S): The patient underwent ovarian tissue cryopreservation for fertility preservation and subsequent heterotopic transplantation for fertility restoration 9 years after freezing...
April 19, 2017: Fertility and Sterility
https://www.readbyqxmd.com/read/28432872/human-effector-memory-t-helper-cells-engage-with-mouse-macrophages-and-cause-graft-versus-host-like-pathology-in-skin-of-humanized-mice-used-in-a-nonclinical-immunization-study
#13
Bala S Sundarasetty, Valery Volk, Sebastian J Theobald, Susanne Rittinghausen, Dirk Schaudien, Vanessa Neuhaus, Constanca Figueiredo, Andreas Schneider, Laura Gerasch, Adele Mucci, Thomas Moritz, Constantin von Kaisenberg, Loukia M Spineli, Katherina Sewald, Armin Braun, Henning Weigt, Arnold Ganser, Renata Stripecke
Humanized mice engrafted with human hematopoietic stem cells and developing functional human T-cell adaptive responses are in critical demand to test human-specific therapeutics. We previously showed that humanized mice immunized with long-lived induced-dendritic cells loaded with the pp65 viral antigen (iDCpp65) exhibited a faster development and maturation of T cells. Herein, we evaluated these effects in a long-term (36 weeks) nonclinical model using two stem cell donors to assess efficacy and safety. Relative to baseline, iDCpp65 immunization boosted the output of effector memory CD4(+) T cells in peripheral blood and lymph nodes...
April 19, 2017: American Journal of Pathology
https://www.readbyqxmd.com/read/28432860/high-ki-67-expression-is-associated-with-prolactin-secreting-pituitary-adenomas
#14
Bogdan Bălinişteanu, Anca Maria Cîmpean, Amalia Raluca Ceauşu, Ana Silvia Corlan, Eugen Melnic, Marius Raica
Pituitary adenomas represent the third most common primary intracranial tumor in neurosurgical practice. To understand the biological behaviour of the pituitary adenomas previous studies have determined the tumor proliferation rate using monoclonal antibodies targeted against the Ki-67 antigen. The aim of this study was to correlate the Ki-67 index with hormonal profiles of pituitary adenomas. The study included 50 pituitary adenoma cases. For histopathologic evaluation, the sections were stained with routine hematoxylin and eosin method...
April 22, 2017: Bosnian Journal of Basic Medical Sciences
https://www.readbyqxmd.com/read/28432801/weight-loss-in-obese-girls-with-polycystic-ovarian-syndrome-is-associated-with-a-decrease-of-anti-muellerian-hormone-concentrations
#15
Thomas Reinehr, Alexandra Kulle, Juliane Rothermel, Caroline Knop, Nina Lass, Christina Bosse, Paul-Martin Holterhus
OBJECTIVE: The Anti- Muellerian Hormone (AMH) has been reported as surrogate marker of antral follicles, which are the origins of hyperandrogenism in polycystic ovarian syndrome (PCOS). Therefore, AMH may be useful for the diagnosis of PCOS. The objective was to study the longitudinal changes in AMH concentrations in girls with and without PCOS. DESIGN: This is a longitudinal study of obese girls participating in a 1-year lifestyle intervention. PATIENTS: Forty obese girls aged 13-16 years (50% with PCOS) were included in the study...
April 22, 2017: Clinical Endocrinology
https://www.readbyqxmd.com/read/28432701/ovarian-follicle-development-and-genital-tract-characteristics-in-different-birthweight-gilts-at-150%C3%A2-days-of-age
#16
Frcl Almeida, Aln Alvarenga Dias, L P Moreira, Atl Fiúza, H Chiarini-Garcia
In the last decades, selection for improved prolificacy has resulted in higher litter sizes and has thereby increased the proportion of low birthweight (LW) piglets. It is well documented that LW piglets have lower growth performance, muscle accretion and poor carcass quality. However, little is known about the relations of birthweight with subsequent reproductive performance in gilts. This study investigated the effects of birthweight on reproductive tract and ovarian follicle development in 150-day-old gilts...
April 22, 2017: Reproduction in Domestic Animals, Zuchthygiene
https://www.readbyqxmd.com/read/28432496/reproductive-effects-of-life-cycle-exposure-to-difenoconazole-on-female-marine-medaka-oryzias-melastigma
#17
Xiaocui Dong, Zhenghong Zuo, Jiaojiao Guo, Hongbin Li, Lemeng Zhang, Meng Chen, Zhibin Yang, Chonggang Wang
Difenoconazole (DFZ) is a widely used triazole fungicide which has been detected in some estuaries and embayments. This study was conducted to investigate the effects of DFZ on ovarian development in female marine medaka (Oryzias melastigma). After 180 days exposure of the embryo to DFZ (0, 1, 10, 100 and 1000 ng/L), the gonadosomatic index and percentage of mature oocytes produced were significantly reduced in the 1, 10 and 100 ng/L treatments but not the 1000 ng/L treatment compared to the control, thus exhibiting a U-shaped dose response curve...
April 21, 2017: Ecotoxicology
https://www.readbyqxmd.com/read/28432272/establishment-of-an-in-vitro-culture-model-of-theca-cells-from-hierarchical-follicles-in-ducks
#18
Xiang Gan, da Chen, Yan Deng, Junsong Yuan, Bo Kang, Jiamin Qiu, Wenqiang Sun, Chunchun Han, Jiwei Hu, Liang Li, Jiwen Wang
Theca cells, including theca interna cells and theca externa cells, are vital components of ovarian follicles. The aim of this study is to identify a reliable method for the in vitro culture of theca cells from duck ovarian hierarchical (F4-F2) follicles. We improved the method for cell separation by using trypsin to further remove granular cells, and we increased the concentration of fetal bovine serum used in in vitro culture to improve cytoactivity. Cell antibody immunofluorescence showed that all inoculated cells could be stained by the CYP17A1/19A1 antibody but not by the FSHR antibody, which could stain granulosa cells...
April 21, 2017: Bioscience Reports
https://www.readbyqxmd.com/read/28432091/suppressive-effects-of-tnf%C3%AE-il6-and-macrophages-on-thecal-androgen-production
#19
Moafaq S Samir, Claire Glister, Dareen Mattar, Mhairi Laird, Philip G Knight
Pro-inflammatory cytokines secreted by macrophages and other cell-types are implicated as intra-ovarian factors affecting different aspects of ovarian function including follicle and corpus luteum 'turnover', steroidogenesis and angiogenesis. Here, we compared granulosal (GC) and thecal (TC) expression of TNF, IL6 and their receptors (TNFR1, TNFRSF1B, IL6R) during bovine antral follicle development; all five mRNA transcripts were detected in both GC and TC and statistically significant cell-type and follicle stage-related differences were evident...
April 21, 2017: Reproduction: the Official Journal of the Society for the Study of Fertility
https://www.readbyqxmd.com/read/28431490/new-challenges-in-therapeutic-vaccines-against-hiv-infection
#20
Lorna Leal, Constanza Lucero, Josep M Gatell, Teresa Gallart, Montserrat Plana, Felipe García
There is a growing interest in developing curative strategies for HIV infection. Therapeutic vaccines are one of the most promising approaches. We will review the current knowledge and the new challenges in this research field. Areas covered: PubMed and ClinicalTrial.gov databases were searched to review the progress and prospects for clinical development of immunotherapies aimed to cure HIV infection. Dendritic cells (DC)-based vaccines have yielded the best results in the field. However, major immune-virologic barriers may hamper current vaccine strategies...
April 21, 2017: Expert Review of Vaccines
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