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Collagen and gut

Takuou Takimoto, Misaki Hatanaka, Tomohiro Hoshino, Tsuyoshi Takara, Ko Tanaka, Atsushi Shimizu, Hiroto Morita, Teppei Nakamura
Gut microbiota influence the host immune system and are associated with various diseases. In recent years, postmenopausal bone loss has been suggested to be related to gut microbiota. In the present study, we investigated the treatment effect of the probiotic Bacillus subtilis C-3102 (C-3102) on bone mineral density (BMD) and its influence on gut microbiota in healthy postmenopausal Japanese women. Seventy-six healthy postmenopausal Japanese women were treated with a placebo or C-3102 spore-containing tablets for 24 weeks...
2018: Bioscience of Microbiota, Food and Health
Ashutosh Kumar, Sanjib K Ghosh, Muneeb A Faiq, Vishwajit R Deshmukh, Chiman Kumari, Vikas Pareek
In the last few years, a cluster of anatomical discoveries has been reported which overturned the long existing dogmas about the structure and function of human body. First to come was the discovery that established the existence of a lymphatic system pertaining to the central nervous system (CNS). CNS was believed to be anatomically immune privileged owing to the absence of any lymphatics and presence of the blood-brain barrier around it, but latest research has established beyond any reasonable doubt that true lymphatic channels carry immune cells in meninges thus challenging the existing theory...
October 18, 2018: QJM: Monthly Journal of the Association of Physicians
Pauline Rivière, Andreas Münch, Pierre Michetti, Nilesh Chande, Gert de Hertogh, Patrick Schoeters, Marc Ferrante, Séverine Vermeire, Gert Van Assche
Background: Evidence for second line therapy in patients with microscopic colitis (MC) failing budesonide is scarce, although anti-tumor necrosing factors (anti-TNFs), methotrexate and azathioprine have been reported to be effective in small cohort studies. Vedolizumab, a monoclonal antibody targeting α4β7-integrin, prevents homing of T-cells to the gut. We evaluated clinical remission with vedolizumab in budesonide-refractory MC patients. Methods: We solicited gastroenterologists in Europe and Canada for cases of MC treated with vedolizumab...
October 17, 2018: Journal of Crohn's & Colitis
Zhiyong Du, Ran Wen, Qian Liu, Jinlong Wang, Yingyuan Lu, Mingbo Zhao, Xiaoyu Guo, Pengfei Tu, Yong Jiang
Cardiac hypertrophy (CH) is a major risk factor for many serious heart diseases. Sustained CH is catastrophic, resulting in cardiac dysfunction that eventually leads to heart failure (HF). Baoyuan decoction (BYD) is a famous traditional Chinese medicine (TCM) formula for supplementing and reinforcing Qi, clinically used for the treatment of cardiovascular diseases (CVDs). However, the therapeutic effects of BYD on CH remain unidentified. We herein investigated the effect of BYD on isoproterenol (ISO)-induced CH in rats and the underlying mechanisms by comprehensive pharmacodynamics and 1 H NMR-based dynamic metabolomics analysis of the plasma and urine samples...
September 29, 2018: Journal of Pharmaceutical and Biomedical Analysis
Huiyong Liu, Honglei Kang, Chao Song, Zuowei Lei, Li Li, Jianfeng Guo, Yong Xu, Hanfeng Guan, Zhong Fang, Feng Li
Low back pain (LBP) is a common worldwide disease that causes an enormous social economic burden. Intervertebral disc degeneration (IDD) is considered as a major cause of LBP. The process of IDD is complicated and involves both inflammation and senescence. The production of pro-inflammatory cytokines, including tumor necrosis factor (TNF)α and interleukin (IL)-1β, is increased in the degenerating intervertebral disc, inducing extracellular matrix degradation. Urolithin A (UA) is a metabolite compound resulting from the degradation of ellagitannins by gut bacteria...
2018: Frontiers in Pharmacology
Vincenza De Gregorio, Giorgia Imparato, Francesco Urciuolo, Paolo A Netti
The small intestine is the major site for digestion, drug and nutrient absorption, as well as a primary site for many diseases. Current in vitro gut models fail in reproducing the complex intestinal extracellular matrix (ECM) network of the lamina propria and the peculiar architecture of the crypt-villus axis. Here we proposed a novel in vitro human intestine model that mimics the intestinal stromal topography and composition and strictly reproduces the tissue polarity with the crypt-villus architecture. First we developed a 3D human intestinal stromal equivalent (3D-ISE) composed of human intestinal subepithelial myofibroblasts (ISEMFs) embedded in their own extracellular matrix...
September 30, 2018: Acta Biomaterialia
Marcel Benadiba, Raphael Serruya, Yehoshua Maor
Hydrolyzed collagen consists of peptides, which exert important biological functions in different body systems. This study aimed at testing the biological effects of a low molecular weight collagen (LMWC), namely Shore Magic® Collagen (SMC), in a series of in vitro assays and three different in vitro barrier models with translational significance. We also compared SMC's biological activities with its trypsinized form (TSMC). SMC enhanced migration in both epithelial and endothelial cells; and increased the adhesion of epithelial cells, but surprisingly not of endothelial cells...
September 2018: Heliyon
Yaqiong Guo, Zhongyu Li, Wentao Su, Li Wang, Yujuan Zhu, Jianhua Qin
Drug metabolism in the intestine is considered to substantially contribute to the overall first-pass metabolism, which has been neglected for a long time. It is highly desirable to develop a reliable model to evaluate drug metabolism in the intestine in vitro. In this work, we made the first attempt to develop a biomimetic human gut-on-a-chip for modeling drug metabolism in intestine. In this chip, constant flow, together with porous nitrocellulose membrane and collagen I, mimics an in vivo-like intestinal microenvironment...
September 5, 2018: Artificial Organs
Waleska Dornas, Denise Glaise, Aude Bodin, Ahmad Sharanek, Audrey Burban, Dounia Le Guillou, Sacha Robert, Stephanie Dutertre, Caroline Aninat, Anne Corlu, Vincent Lagente
Previous studies have shown that gut-derived bacterial endotoxins contribute in the progression of simple steatosis to steatohepatitis, although the mechanism(s) remains inaccurate to date. As hepatic stellate cells (HSC) play a pivotal role in the accumulation of excessive extracellular matrix (ECM), leading to collagen deposition, fibrosis, and perpetuation of inflammatory response, an in vitro model was developed to investigate the crosstalk between HSC and hepatocytes (human hepatoma cell) pretreated with palmitate...
January 2018: Journal of Cellular Physiology
Nina Kimer, Natasja Stæhr Gudmann, Julie Steen Pedersen, Søren Møller, Mette Juul Nielsen, Diana Julie Leeming, Morten Asser Karsdal, Holger Jon Møller, Flemming Bendtsen, Henning Grønbæk
BACKGROUND AND AIMS: Macrophages play a significant role in chronic liver disease as reflected by elevated soluble (s)CD163 and mannose receptor (sMR) levels and associated with liver disease severity and prognosis. Extracellular matrix remodelling associated with fibrogenesis may be affected by systemic inflammation induced by bacterial translocation. Therefore, we aimed to investigate the effect of rifaximin-α, an antibiotic with effect on gut bacteria, on sCD163, sMR, and collagen metabolites...
2018: PloS One
Kristian H Mikkelsen, Tina Vilsbøll, Jens J Holst, Bolette Hartmann, Filip K Knop, Morten Frost
BACKGROUND: Intestinal bacteria influence bone remodeling in rodents, and antibiotic manipulation of the rodent gut microbiota increases bone formation and prevents ovariectomy-induced bone loss. In theory, these effects may be mediated by changes in sex hormone biotransformation in the gut, gut serotonin secretion or nutrition-induced secretion of glucagon-like peptide 2 (GLP-2) and glucose-dependent insulinotropic hormone (GIP). Antibiotics change the human gut microbiota, but the effect of antibiotic treatment on human bone turnover is unknown...
September 4, 2018: BMC Endocrine Disorders
Qian Wang, Xin Sui, Rui Chen, Pei-Yong Ma, Yong-Liang Teng, Tao Ding, Dian-Jun Sui, Ping Yang
Angiotensin (Ang) II contributes to the formation and development of myocardial fibrosis. Ghrelin, a gut peptide, has demonstrated beneficial effects against cardiovascular disease. In the present study, we explored the effect and related mechanism of Ghrelin on myocardial fibrosis in Ang II-infused rats. Adult Sprague-Dawley (SD) rats were divided into 6 groups: Control, Ang II (200ng/kg/min, microinfusion), Ang II+Ghrelin (100 μ g/kg, subcutaneously twice daily), Ang II+Ghrelin+GW9662 (a specific PPAR- γ inhibitor, 1 mg/kg/d, orally), Ang II+GW9662, and Ghrelin for 4 wks...
2018: BioMed Research International
David Feder, Mariana Ierardi, Ana Laura Covre, Giuliana Petri, Alzira Alves de Siqueira Carvalho, Fernando Luiz Affonso Fonseca, Bruno Machado Bertassoli
This study describes the functional and morphological alterations in the intestines of mdx mice (n = 4) compared with the intestinal features of C57BL/10 mice (n = 7) at 2 months of age. The whole gut transit time (carmine red) and the upper gut transit time (activated charcoal) were measured, and light microscopy was utilized to view stained sections (H&E and picrosirius red) for histological analysis. No significant difference in mean evacuation time for the whole gut was observed between the two groups, but a significant delay in activated charcoal passage was observed in the mdx mice...
August 2018: APMIS: Acta Pathologica, Microbiologica, et Immunologica Scandinavica
Man Xiao, Xiaopeng Fu, Yali Ni, Jifeng Chen, Shaoqing Jian, Liankai Wang, Li Li, Guankui Du
ETHNOPHARMACOLOGICAL RELEVANCE: Paederia scandens (Lour.) Merr. (P. scandens) has been traditionally used to treat the pain of rheumatism. AIM OF THE STUDY: The purpose of this study was to investigate the possible influences of P. scandens on the progression of rheumatoid arthritis (RA), inflammatory responses and gut bacterial communities in RA mouse model. MATERIALS AND METHODS: collagen-induced arthritis (CIA) mice were orally administered with P...
November 15, 2018: Journal of Ethnopharmacology
MengFan Yue, XinYu Zhang, YanNong Dou, ZhiFeng Wei, Yu Tao, YuFeng Xia, Yue Dai
Sinomenine has long been used for the treatment of rheumatoid arthritis in China. However, its anti-inflammatory mechanism is still debatable because the in vitro minimal effective concentration (≥250 μM) is hardly reached in either synovium or serum after oral administration at a therapeutic dose. Recent findings suggest that the α7 nicotinic acetylcholine receptor (α7nAChR) might mediate the inhibitory effect of sinomenine on macrophage activation, which attracts us to explore the anti-arthritis mechanism of sinomenine by taking neuroendocrine-inflammation axis into consideration...
2018: Frontiers in Pharmacology
Noam Jacob, Jonathan P Jacobs, Kotaro Kumagai, Connie W Y Ha, Yoshitake Kanazawa, Venu Lagishetty, Katherine Altmayer, Ariel M Hamill, Aimee Von Arx, R Balfour Sartor, Suzanne Devkota, Jonathan Braun, Kathrin S Michelsen, Stephan R Targan, David Q Shih
Tumor necrosis factor-like cytokine 1A (TL1A, TNFSF15) is implicated in inflammatory bowel disease (IBD), modulating the location and severity of intestinal inflammation and fibrosis. TL1A expression is increased in inflamed gut mucosa and associated with fibrostenosing Crohn's disease. Tl1a-overexpression in mice lead to spontaneous ileitis, and exacerbated induced proximal colitis and fibrosis. IBD is associated with shifts in the gut microbiome, but the effect of differing microbial populations and their interaction with TL1A on fibrosis has not been investigated...
September 2018: Mucosal Immunology
Shukai Yang, Huijuan Chen, Bo Wei, Min Xiang, Zibing Hu, Zhiheng Peng, Hao Lin, Jiecong Sun
The profound influence of gut flora on host immune system and its link with autoimmune disorders have been established. However, the role of certain antibiotic in progression of autoimmune disorder is still confusing. Here, we employed a collagen-induced arthritis (CIA) model to explore the role of clindamycin administration in different scenarios. In the first scenario, mice treated with antibiotics for 4 weeks were performed with the induction of CIA immediately. The results showed that clindamycin administration promoted the incidence and severity of CIA, while the recipients of vancomycin showed completed tolerance...
October 2018: Inflammation
Matthew R Gemmell, Susan Berry, Indrani Mukhopadhya, Richard Hansen, Hans L Nielsen, Mona Bajaj-Elliott, Henrik Nielsen, Georgina L Hold
In recent years, an increasing number of Campylobacter species have been associated with human gastrointestinal (GI) diseases including gastroenteritis, inflammatory bowel disease, and colorectal cancer. Campylobacter concisus, an oral commensal historically linked to gingivitis and periodontitis, has been increasingly detected in the lower GI tract. In the present study, we generated robust genome sequence data from C. concisus strains and undertook a comprehensive pangenome assessment to identify C. concisus virulence properties and to explain potential adaptations acquired while residing in specific ecological niche(s) of the GI tract...
June 26, 2018: Emerging Microbes & Infections
Poonam Giri, Prashant Delvadia, Lakshmikant Gupta, Priyal Trivedi, Nirmal Patel, Manoranjan Sharma, Jaideep Singh, Abhijit Chatterjee, Shekhar Kadam, Nuggehally R Srinivas
Pharmacokinetics of voriconazole, an anti-fungal agent, was determined in collagen-induced arthritic (CIA) and healthy DBA/1J mice. CIA was confirmed in DBA/1J mice by clinical scoring and histological analysis. In vivo oral pharmacokinetic study (3 mg/kg) and in vitro stability assessment in liver microsomes were performed in CIA vs. healthy DBA/1J mice. Additionally, hepatic portal vein cannulated (HPVC) CIA and healthy mice were used to clarify the role of gut first-pass effect. Voriconazole/N-oxide metabolite was measured in plasma and in vitro samples using liquid chromatography tandem-mass spectrometry method...
July 30, 2018: Xenobiotica; the Fate of Foreign Compounds in Biological Systems
Jian Xu, Jinlong Liu, Guolin Yue, Mingqiang Sun, Jinliang Li, Xia Xiu, Zhenzhong Gao
A series of natural compounds have been implicated to be useful in regulating the pathogenesis of various autoimmune diseases. The present study demonstrated that the Scutellariae radix compounds baicalein and baicalin may serve as drugs for the treatment of autoimmune diseases, including rheumatoid arthritis and inflammatory bowel disease. Following the administration of baicalein and baicalin in vivo, T cell‑mediated autoimmune diseases in the mouse model were profoundly ameliorated: In the collagen‑induced arthritis model (CIA), the severity of the disease was reduced by baicalein and, consistently, baicalein was demonstrated to suppress T cell proliferation in CIA mice...
July 2018: Molecular Medicine Reports
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