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Egg allergens

Ondulla T Toomer, Amanda M Hulse-Kemp, Lisa L Dean, Deborah L Boykin, Ramon Malheiros, Kenneth E Anderson
Previous studies have identified normal-oleic peanuts as a suitable and economical broiler feed ingredient. However, no studies to date have examined the use of high-oleic (HO) peanut cultivars as a feed ingredient for laying hens and determined the impact of feeding HO peanuts on performance and egg nutritive qualities. This project aimed to examine the use of HO peanuts as a feed ingredient for layer hens to determine the effect on performance, egg lipid chemistry, and quality of the eggs produced. Forty-eight 40-wk-old layer hens were fed a conventional soybean meal + corn control diet or a HO peanut + corn diet for 10 wk in conventional battery cages...
December 11, 2018: Poultry Science
Junpu Li, Shaoshen Li, Lunhui Huang, Yaqiong Cui, Tiantian She, Ying Bian, Huiqiang Li
BACKGROUND: An increase in allergen-specific IgG4 (sIgG4 ), which serves as a blocking antibody, is associated with acquisition of immune tolerance after immunotherapy. In this study, we developed a rapid, sensitive, and homogeneous immunoassay based on the light-initiated chemiluminescent assay (LICA) technology for quantifying allergen sIgG4 in serum samples. METHODS: Allergen sIgG4 was measured in vitro by incubating the sample with biotinylated allergens and chemiluminescent beads coated with anti-human IgG4 antibody, followed by the addition of streptavidin-coated sensitizer beads...
December 2, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
William L Nowatzke, Kerry G Oliver, Chung Y Cho, Prasad Rallabhandi, Eric A E Garber
An xMAP Food Allergen Detection Assay (xMAP FADA) was developed to meet analytical needs when responding to complaints by individuals with multiple food allergies and to address potential ambiguities associated with cross-reactive proteins. A Single Laboratory Validation (SLV) was conducted to examine the reliability of the xMAP FADA to detect 15 analytes individually, or as part of a mixture, at ≥6 concentrations, in four foods. The xMAP FADA reliably detected the analytes despite the incurred dark chocolate and incurred baked muffins displaying recoveries of 10-20% and <60%, respectively...
November 28, 2018: Journal of Agricultural and Food Chemistry
Kiwako Yamamoto-Hanada, Tohru Kobayashi, Hywel C Williams, Masashi Mikami, Mayako Saito-Abe, Kumiko Morita, Osamu Natsume, Miori Sato, Motoko Iwama, Yumiko Miyaji, Makiko Miyata, Shinichiro Inagaki, Fukuie Tatsuki, Narita Masami, Shoji F Nakayama, Hiroshi Kido, Hirohisa Saito, Yukihiro Ohya
Background: Atopic dermatitis is the first clinical manifestation of the atopic march, with the highest incidence in the first year of life. Those affected often go on to develop other allergic diseases including food allergy, asthma, and allergic rhinitis. Recent evidence suggests that sensitization to foods may occur through a defective skin barrier which is common in atopic dermatitis in early life. We hypothesize that therapeutic aggressive intervention to treat new onset atopic dermatitis may prevent the development of later allergen sensitization, and associated food allergy, asthma, and allergic rhinitis...
2018: Clinical and Translational Allergy
M Gavage, K Van Vlierberghe, C Van Poucke, M De Loose, K Gevaert, M Dieu, P Renard, T Arnould, N Gillard
Food allergy is a growing health problem worldwide; thus, there is an urgent need for robust, specific, and sensitive analytical methods for detecting allergens. Mass spectrometry is an alternative to the existing methods, and it can overcome their limitations. One of the first steps in the development of any analytical method is the identification of the analytes to be further studied. In the case of allergen detection by mass spectrometry, the analytes are peptides. In this study, a strategy was developed for identifying potential peptide biomarkers in processed food products...
November 20, 2018: Journal of Chromatography. A
Małgorzata Bulanda, Magdalena Leśniak, Judyta Szałkowska, Weronika Madej, Agata Wojtyna, Maria Czarnobilska, Daniel Bulanda, Ewa Czarnobilska
Anaphylaxis is an increasing problem in public health. The food allergens (mainly milk, eggs, and peanuts) are the most frequent cause of anaphylaxis in children and youth. In order to define the cause of anaphylaxis, skin tests, the determination of the concentration of specific IgE in the blood and basophil activation test are conducted. In vitro tests are preferred due to the risk of allergic response during in vivo tests. Component-resolved diagnosis (CRD) is an additional tool in allergology, recommended in the third level of diagnostics when there are diagnostic doubts after the above mentioned tests have been carried out...
2018: Folia Medica Cracoviensia
Carlo Caffarelli, Dora Di Mauro, Carla Mastrorilli, Paolo Bottau, Francesca Cipriani, Giampaolo Ricci
The rise of food allergy in childhood, particularly among developed countries, has a significant weight on public health and involves serious implications for patients' quality of life. Even if the mechanisms of food tolerance and the complex interactions between the immune system and environmental factors are still mainly unknown, pediatricians have worldwide implemented preventive measures against allergic diseases. In the last few decades, the prevention of food allergy has tracked various strategies of complementary feeding with a modification of international guidelines from delayed introduction to early weaning...
November 17, 2018: Nutrients
Shinsuke Hidese, Shun Nogawa, Kenji Saito, Hiroshi Kunugi
BACKGROUND: Although some studies suggest a possible association between food allergy (FA) and depression/psychological distress, empirical data are still scarce. We aimed to examine whether this association exists in a large sample of the Japanese population. METHODS: This web-based cross-sectional survey included 1000 individuals with self-reported lifetime history of clinical depression (mean age: 41.4 years, 501 men), and the remaining 10,876 subjects served as controls (mean age: 45...
October 26, 2018: Journal of Affective Disorders
Mahshid Sirjani, Raheleh Shokouhi Shoormasti, Nastaran Sabetkish, Maryam Ayazi, Nazanin Khodayari Namini, Behnoosh Tayebi, Zahra Pourpak
We aimed to assess the possible relationship between food allergy and two key adipokines - leptin and adiponectin - in children with food allergy. A total of forty patients with definite diagnosis of food allergy according to clinical history and specific IgE (sIgE) for food allergens (group I) were enrolled in this pilot study. The control group (group II) included thirty children with no evidence of allergic symptoms. Serum levels of leptin and adiponectin were measured by ELISA. Meanwhile, sIgE was measured for the eight most common food allergens by the immunoblot method in all participants...
November 2018: British Journal of Nutrition
H Y Wang, Z W Cai
Objective: To explore the distribution and characteristics of allergens in children aged 0-12 years old in liaoning province. Method: 46 620 children with suspected allergic diseases were grouped according to age, sex and detection time. Then allergen detections were conducted. The distribution of allergen in different groups was analyzed. Result: Most children were sensitized to single allergen, followed by a double positive. 98.08% children showed sensitivities to up to five kinds of allergen. The allergen spectrum of patients in different age groups was slightly different...
October 2018: Journal of Clinical Otorhinolaryngology, Head, and Neck Surgery
Jinyoung Yang, Hyeyoung Lee, Ae Ran Choi, Ki Hyun Park, Ji Hyeong Ryu, Eun-Jee Oh
In vitro allergen-specific immunoglobulin E (sIgE) measurement has been used as an important diagnostic tool for allergic diseases. Currently, quantitative sIgE levels are detected mainly by using ImmunoCAP and Immulite 2000 assay system. These two systems have the same calibration scale at 0-100 kUA/L, but they differ in used allergens, detection methods and automation systems. We compared 1600 paired sIgE results for 204 allergic patients, including 100 paired sIgE assay results for each of 16 allergens (Alternaria alternata, birch-alder mix, cat dander, D...
October 30, 2018: Scandinavian Journal of Clinical and Laboratory Investigation
Magdalena Montowska, Emilia Fornal
We present an implementation of the absolute quantification (AQUA) method for monitoring of peptide abundance in complex mixtures of processed proteins. Specific peptide markers from meats (chicken, duck, goose, pork and beef) and common protein allergenic additives (soy, milk and egg white preparations) were chosen and synthesised with stable isotopes (13 C and 15 N) for use as internal standards. A wide range of food samples, from cooked or raw meat to sterilised pâté, was analysed by a triggered multiple reaction monitoring mode experiment and triple quadrupole mass spectrometry for the direct measure of tryptic peptides representing the amounts of specific proteins...
February 15, 2019: Food Chemistry
M Planque, T Arnould, P Delahaut, P Renard, M Dieu, N Gillard
Worldwide, mass spectrometry is widely used to detect and quantify food allergens, especially in complex and processed food products. Yet, the absence of a regulatory framework for the developed methods has led to a lack of harmonization between laboratories. In this study, ten allergens were analyzed in eight food products by UHPLC-MS/MS, in order to establish criteria for the retention time, variation tolerance, the ion ratio deviation, and the signal-to-noise ratio for allergen detection. The set of criteria should help laboratories to compare results and avoid false positives and negatives...
February 15, 2019: Food Chemistry
A A Eapen, K M Kloepfer, F E Leickly, J E Slaven, G Vitalpur
BACKGROUND: Recent studies have suggested that removing foods from the diet to manage atopic dermatitis (AD), based on positive allergy tests, may lead to immediate allergic reactions on reintroduction of that food. OBJECTIVE: The purpose of this study was to examine the frequency of oral food challenge (OFC) failures among foods removed from the diet as suspected AD triggers, focusing on the five major food allergens in the US. METHODS: OFCs to egg, milk, peanut, soy, and wheat, performed from 2008-14, at a children's hospital's allergy clinics, were reviewed...
October 13, 2018: Annals of Allergy, Asthma & Immunology
Natasha Purington, R Sharon Chinthrajah, Andrew Long, Sayantani Sindher, Sandra Andorf, Katherine O'Laughlin, Margaret A Woch, Alexandra Scheiber, Amal Assa'ad, Jacqueline Pongracic, Jonathan M Spergel, Jonathan Tam, Stephen Tilles, Julie Wang, Stephen J Galli, Manisha Desai, Kari C Nadeau
Background: Food allergy prevalence has continued to rise over the past decade. While studies have reported threshold doses for multiple foods, large-scale multi-food allergen studies are lacking. Our goal was to identify threshold dose distributions and predictors of severe reactions during blinded oral food challenges (OFCs) in multi-food allergic patients. Methods: A retrospective chart review was performed on all Stanford-initiated clinical protocols involving standardized screening OFCs to any of 11 food allergens at 7 sites...
2018: Frontiers in Immunology
Manuel Jorge Rial, Marcela Valverde-Monge, Aida Gómez-Cardeñosa, Rosialzhira Vera-Berríos, Carlos Pastor-Vargas, Javier Cuesta-Herranz
No abstract text is available yet for this article.
October 5, 2018: Journal of Allergy and Clinical Immunology in Practice
Michael S Benninger, Thomas Daly, Kevin Graffmiller
Rates of allergy-test positivity vary by country and by regions within countries. Several studies have looked at allergy test results to determine the most common allergens. Many of these studies have been based on surveys or on studies of small numbers of tests. Positivity rates for allergy tests are poorly defined in the northern midwestern region of the United States. We conducted a study to identify the rates of positive allergy tests for both inhalant/respiratory allergens and food allergens in the upper Midwest...
September 2018: Ear, Nose, & Throat Journal
Runar Almaas, Svanhildur Haflidadottir, Runa Helen Kaldestad, Iren Lindbak Matthews
OBJECTIVES: To describe the prevalence and the relationship between asthma, eczema, food allergy, and rhinitis in children after liver transplantation. STUDY DESIGN: Children who were liver transplant recipients were investigated to assess whether the high prevalence of food allergies was accompanied by eczema, rhinitis, and asthma. Furthermore, we included 56 children with chronic liver disease to explore the risk of allergy, eczema, and asthma in this group. RESULTS: After liver transplantation, children had higher prevalence of allergic reactions to food as compared with children with chronic liver disease (P < ...
September 27, 2018: Journal of Pediatrics
Susan Waserman, Philippe Bégin, Wade Watson
Food allergy is defined as an adverse immunologic response to a food protein. Food-related reactions are associated with a broad range of signs and symptoms that may involve any body system, including the skin, gastrointestinal and respiratory tracts, and cardiovascular system. Immunoglobulin E (IgE)-mediated food allergy is a leading cause of anaphylaxis and, therefore, referral to an allergist for timely and appropriate diagnosis and treatment is imperative. Diagnosis entails a careful history and diagnostic tests, such as skin prick tests, serum-specific IgE and, if indicated, an oral food challenge...
2018: Allergy, Asthma, and Clinical Immunology
Zi-Wei Liao, Yun-Hua Ye, Hui Wang, Yang Chen, Xiao-Mei Sha, Lu Zhang, Tao Huang, Yue-Ming Hu, Zong-Cai Tu
Ovalbumin is one of the most important sensitizing ingredients in allergens of egg albumin, which restricts the application of egg in the field of food processing. Previous research has indicated that glycation could cause the protein to partially expand, which may bring about the destruction of the structural IgG and IgE epitopes and induce the decline of the IgG- and IgE-binding ability of ovalbumin. In this research, the effect of a preheating treatment integrated with glycation on the IgG- and IgE-binding capability and the conformation changes of ovalbumin was studied by detecting the glycated sites and the values of degree of substitution per peptide (DSP) by liquid chromatography and high-resolution mass spectrometry (LC-HRMS)...
October 5, 2018: Journal of Agricultural and Food Chemistry
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